Rmu_sc0001171.1_g000012

Translation initiation factor eIF-2B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001171.1
OG0005877
Physical Location & Seq
Forward (+)
41950 .. 51537
9588 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1815 bp
atgggggcacaaaagaaaggcgcgtctagggcttcagaggaccctgaggaactggcccgtctcccactccaagccatcctcttagccgatagtttcaccaccaaattccgacccatcacccttgaacgccccaaagtgctgttgccgttagtaaatgccccgatgatcagttacacattagcatggcttgagtctgctggcgttgaggaggtttttgttgtctgctgtgctcactccaagcaagtgatcagttatttggagaattctgagtggttttctcaaccaaacttcacggtcaccacaattgagtcacataattctgttagtgccggagatgctctgcgcgtgatatatgagcgcaatgtggattgctacatcgacatatgctctcctgaagtcctcagccttttcacagacaattttgactatcaccatttacgctgtcattttttaaagggattgcttgttgatgatattatgggttacaaaatattcacacatgaaattcactcgacttatgcggctaggattgacaacttcagaagctatgacacaatcagtagggacataattcagagatgggcttacccattggtgccagatgttaagtttttcagagattcttcaacgaagctggaaagacaggggatgtatctagtcacagaactagggcaatcgcgctctgcacagattggtccttttacagtcattgggaatggcaccaagattggggacaacaccaaaatttcaaattctgttattggagatgggtgttctattggaccaaacgtttccatagaaggttcctatatatgggataatgtcaccattgaagatggctgcaagataaggcatgctatagtatgtgatggagtgataatgaagtctggggcagttttggaaccgggtgtagttttgtctttcaaggtagtgataggacaacaatttattgctccttcatacttaatggtatctttactccaacaaccaataaagcaaaatagtgatgagaagctagagtatgttgacaataccactgctcttacacatgcactggatatatcgaatggggaaatagcgaaccaacaacaggagacacagtgttgggcgacatctgagcttggtattggtggggctggttatgtttggtcaatttgtgaaggaggccatgatgaaggatggaggcattcagttgctcccattcccacagagaaacttgctgaggttatacaagatgcagaggatgatcatgatggcagtctcccaacttcagattctgattctgatgaagagcctagagacgactcctactttgaaaatgaggttgaagcaaccttcctaagagctgtggatgaaaatattaaagtagagaatgtcgttttagaagtgagctcactgagattgtcatacaacaagcaaactgcaaactgtgctggagctatattttattcaatgatgaaattggcattagaatctccacataattcagcaggtgaattggtccaagctactatgaaggtaattacaaaatggaaaggactgctgaagtattacctatcagacatcgatgaagagatagaagtaataatgaaatttgaagatatgtgtttggagtccgctaaggagttctcccctgtgtttgataagatactctatcaattgtatgaccacacaattatacgggaagaggctattctaaggtgggatgatgaaaagaaagatgctgatgcatcagatagaatttttgttaggcaggctgaagattttatccaatggttgagagaggcatctgaagaagaggatgaggaagagtag

Protein Analysis

604

Amino Acids

67.94

Weight (kDa)

4.59

Isoelectric Point (pI)

44.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTP_transferase PF00483 25 - 100 8.4e-07 Nucleotidyl transferase
LbH_EIF2B PF25084 214 - 316 9.6e-39 EIF2B subunit epsilon LbH domain
GMPPB_C PF25087 227 - 311 2.2e-10 GMPPB C-terminal domain
Hexapep PF00132 235 - 268 7.5e-08 Bacterial transferase hexapeptide (six repeats)
Hexapep_GlmU PF24894 236 - 299 5.4e-06 GlmU-like, C-terminal hexapeptide
W2 PF02020 525 - 603 5.2e-17 eIF4-gamma/eIF5/eIF2-epsilon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1481
Acc36I ACCTGC 1 cut(s) 1481
AccB1I GGYRCC 2 cut(s) 595, 719
AccII CGCG 3 cut(s) 23, 345, 679
AciI CCGC 2 cut(s) 521, 1617
AclI AACGTT 1 cut(s) 789
AcsI RAATTY 7 cut(s) 104, 262, 504, 744, 751, 1592, 1740
AcuI CTGAAG 7 cut(s) 18, 414, 523, 1254, 1565, 1779, 1812
AfiI CCNNNNNNNGG 3 cut(s) 729, 813, 1090
AgsI TTSAA 9 cut(s) 125, 627, 750, 833, 925, 1316, 1328, 1452, 1598
AjuI GAANNNNNNNTTGG 2 cut(s) 1258, 1290
AluBI AGCT 8 cut(s) 546, 634, 1015, 1120, 1346, 1392, 1439, 1508
AluI AGCT 8 cut(s) 546, 634, 1015, 1120, 1346, 1392, 1439, 1508
Alw21I GWGCWC 2 cut(s) 232, 1394
Alw26I GTCTC 4 cut(s) 65, 1088, 1265, 1293
AlwNI CAGNNNCTG 1 cut(s) 1277
AoxI GGCC 2 cut(s) 54, 1165
ApeKI GCWGC 1 cut(s) 840
ApoI RAATTY 7 cut(s) 104, 262, 504, 744, 751, 1592, 1740
ArsI GACNNNNNNTTYG 2 cut(s) 1295, 1327
AspLEI GCGC 4 cut(s) 23, 345, 360, 681
AspS9I GGNCC 5 cut(s) 40, 55, 695, 782, 1501
AsuC2I CCSGG 1 cut(s) 906
AsuHPI GGTGA 6 cut(s) 88, 109, 289, 422, 817, 1505
AvaII GGWCC 4 cut(s) 40, 695, 782, 1501
AxyI CCTNAGG 1 cut(s) 45
BaeGI GKGCMC 1 cut(s) 10
BanI GGYRCC 2 cut(s) 595, 719
BanII GRGCYC 1 cut(s) 1394
Bbv12I GWGCWC 2 cut(s) 232, 1394
BbvCI CCTCAGC 2 cut(s) 401, 1221
BbvI GCAGC 1 cut(s) 827
BccI CCATC 8 cut(s) 83, 122, 573, 761, 830, 863, 1173, 1247
BceAI ACGGC 1 cut(s) 130
BclI TGATCA 3 cut(s) 165, 246, 1246
BcnI CCSGG 1 cut(s) 906
BcoDI GTCTC 4 cut(s) 65, 1088, 1265, 1293
BfaI CTAG 6 cut(s) 27, 525, 656, 668, 1016, 1296
BfmI CTRYAG 1 cut(s) 858
BfuAI ACCTGC 1 cut(s) 1481
BisI GCNGC 2 cut(s) 522, 841
BlsI GCNGC 2 cut(s) 523, 842
Bme1390I CCNGG 1 cut(s) 906
Bme18I GGWCC 4 cut(s) 40, 695, 782, 1501
BmgT120I GGNCC 5 cut(s) 40, 55, 695, 782, 1501
BmiI GGNNCC 5 cut(s) 42, 597, 721, 805, 903
BmrFI CCNGG 1 cut(s) 906
BmsI GCATC 6 cut(s) 325, 1225, 1711, 1717, 1739, 1796
BpmI CTGGAG 1 cut(s) 1455
Bpu10I CCTNAGC 3 cut(s) 401, 1221, 1620
BpuEI CTTGAG 1 cut(s) 209
BpuMI CCSGG 1 cut(s) 906
Bsa29I ATCGAT 1 cut(s) 1566
BsaXI ACNNNNNCTCC 2 cut(s) 1085, 1115
Bsc4I CCNNNNNNNGG 3 cut(s) 729, 813, 1090
Bse1I ACTGG 2 cut(s) 57, 1059
Bse21I CCTNAGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 367
BseCI ATCGAT 1 cut(s) 1566
BseGI GGATG 7 cut(s) 75, 654, 1184, 1249, 1357, 1711, 1807
BseLI CCNNNNNNNGG 3 cut(s) 729, 813, 1090
BseMI GCAATG 1 cut(s) 367
BseMII CTCAG 6 cut(s) 36, 258, 415, 1107, 1212, 1388
BseNI ACTGG 2 cut(s) 57, 1059
BseRI GAGGAG 1 cut(s) 221
BseSI GKGCMC 1 cut(s) 10
BseXI GCAGC 1 cut(s) 827
BsgI GTGCAG 1 cut(s) 669
Bsh1236I CGCG 3 cut(s) 23, 345, 679
BshFI GGCC 2 cut(s) 56, 1167
BshNI GGYRCC 2 cut(s) 595, 719
BshVI ATCGAT 1 cut(s) 1566
BsiHKAI GWGCWC 2 cut(s) 232, 1394
BsiSI CCGG 2 cut(s) 330, 905
BslFI GGGAC 2 cut(s) 578, 746
BslI CCNNNNNNNGG 3 cut(s) 729, 813, 1090
BsmAI GTCTC 4 cut(s) 65, 1088, 1265, 1293
BsmBI CGTCTC 2 cut(s) 65, 1293
BsmFI GGGAC 2 cut(s) 578, 746
BsmI GAATGC 1 cut(s) 1186
BsnI GGCC 2 cut(s) 56, 1167
Bsp1286I GDGCHC 3 cut(s) 10, 232, 1394
Bsp143I GATC 3 cut(s) 165, 246, 1246
BspACI CCGC 2 cut(s) 521, 1617
BspANI GGCC 2 cut(s) 56, 1167
BspCNI CTCAG 6 cut(s) 37, 259, 414, 1108, 1213, 1389
BspDI ATCGAT 1 cut(s) 1566
BspFNI CGCG 3 cut(s) 23, 345, 679
BspHI TCATGA 1 cut(s) 1249
BspLI GGNNCC 5 cut(s) 42, 597, 721, 805, 903
BspMI ACCTGC 1 cut(s) 1481
BspQI GCTCTTC 1 cut(s) 1284
BspT107I GGYRCC 2 cut(s) 595, 719
BsrDI GCAATG 1 cut(s) 367
BsrI ACTGG 2 cut(s) 57, 1059
BssMI GATC 3 cut(s) 165, 246, 1246
Bst4CI ACNGT 4 cut(s) 295, 706, 1101, 1430
Bst6I CTCTTC 5 cut(s) 1284, 1566, 1680, 1791, 1803
BstAPI GCANNNNNTGC 1 cut(s) 1430
BstC8I GCNNGC 3 cut(s) 199, 855, 1755
BstEII GGTNACC 1 cut(s) 295
BstF5I GGATG 7 cut(s) 75, 654, 1184, 1249, 1357, 1711, 1807
BstFNI CGCG 3 cut(s) 23, 345, 679
BstHHI GCGC 4 cut(s) 23, 345, 360, 681
BstKTI GATC 3 cut(s) 168, 249, 1249
BstMAI GTCTC 4 cut(s) 65, 1088, 1265, 1293
BstMBI GATC 3 cut(s) 165, 246, 1246
BstMWI GCNNNNNNNGC 3 cut(s) 29, 335, 1430
BstNSI RCATGY 2 cut(s) 857, 1052
BstPI GGTNACC 1 cut(s) 295
BstSCI CCNGG 1 cut(s) 904
BstSFI CTRYAG 1 cut(s) 858
BstSLI GKGCMC 1 cut(s) 10
BstUI CGCG 3 cut(s) 23, 345, 679
BstV1I GCAGC 1 cut(s) 827
Bsu15I ATCGAT 1 cut(s) 1566
Bsu36I CCTNAGG 1 cut(s) 45
BsuRI GGCC 2 cut(s) 56, 1167
BsuTUI ATCGAT 1 cut(s) 1566
BtsCI GGATG 7 cut(s) 75, 654, 1184, 1249, 1357, 1711, 1807
BtsI GCAGTG 1 cut(s) 1035
BtsIMutI CAGTG 4 cut(s) 1035, 1052, 1106, 1394
BveI ACCTGC 1 cut(s) 1481
Cac8I GCNNGC 3 cut(s) 199, 855, 1755
CaiI CAGNNNCTG 1 cut(s) 1277
CciI TCATGA 1 cut(s) 1249
CfoI GCGC 4 cut(s) 23, 345, 360, 681
Cfr13I GGNCC 5 cut(s) 40, 55, 695, 782, 1501
ClaI ATCGAT 1 cut(s) 1566
CseI GACGC 1 cut(s) 12
CviAII CATG 6 cut(s) 183, 500, 854, 1049, 1169, 1250
DpnI GATC 3 cut(s) 167, 248, 1248
DpnII GATC 3 cut(s) 165, 246, 1246
DraI TTTAAA 1 cut(s) 453
Eam1104I CTCTTC 5 cut(s) 1284, 1566, 1680, 1791, 1803
EarI CTCTTC 5 cut(s) 1284, 1566, 1680, 1791, 1803
Ecl136II GAGCTC 1 cut(s) 1392
Eco24I GRGCYC 1 cut(s) 1394
Eco47I GGWCC 4 cut(s) 40, 695, 782, 1501
Eco53kI GAGCTC 1 cut(s) 1392
Eco57I CTGAAG 7 cut(s) 18, 414, 523, 1254, 1565, 1779, 1812
Eco81I CCTNAGG 1 cut(s) 45
Eco91I GGTNACC 1 cut(s) 295
EcoICRI GAGCTC 1 cut(s) 1392
EcoO109I RGGNCCY 1 cut(s) 40
EcoO65I GGTNACC 1 cut(s) 295
EcoRI GAATTC 1 cut(s) 262
EcoT22I ATGCAT 1 cut(s) 1732
EcoT38I GRGCYC 1 cut(s) 1394
Esp3I CGTCTC 2 cut(s) 65, 1293
FaeI CATG 6 cut(s) 186, 503, 857, 1052, 1172, 1253
FaqI GGGAC 2 cut(s) 578, 746
FatI CATG 6 cut(s) 182, 499, 853, 1048, 1168, 1249
FauNDI CATATG 1 cut(s) 383
FbaI TGATCA 3 cut(s) 165, 246, 1246
Fnu4HI GCNGC 2 cut(s) 522, 841
FokI GGATG 6 cut(s) 62, 661, 1191, 1256, 1364, 1718
FriOI GRGCYC 1 cut(s) 1394
Fsp4HI GCNGC 2 cut(s) 522, 841
FspBI CTAG 6 cut(s) 27, 525, 656, 668, 1016, 1296
GlaI GCGC 4 cut(s) 22, 344, 359, 680
GluI GCNGC 2 cut(s) 522, 841
GsuI CTGGAG 1 cut(s) 1455
HaeIII GGCC 2 cut(s) 56, 1167
HapII CCGG 2 cut(s) 330, 905
HgaI GACGC 1 cut(s) 12
HhaI GCGC 4 cut(s) 23, 345, 360, 681
Hin1II CATG 6 cut(s) 186, 503, 857, 1052, 1172, 1253
Hin6I GCGC 4 cut(s) 21, 343, 358, 679
HinP1I GCGC 4 cut(s) 21, 343, 358, 679
HincII GTYRAC 1 cut(s) 1027
HindII GTYRAC 1 cut(s) 1027
HinfI GANTC 8 cut(s) 191, 308, 620, 1274, 1280, 1304, 1472, 1613
HpaII CCGG 2 cut(s) 330, 905
HphI GGTGA 6 cut(s) 88, 109, 289, 422, 817, 1505
Hpy166II GTNNAC 1 cut(s) 1027
Hpy188III TCNNGA 2 cut(s) 392, 1250
Hpy8I GTNNAC 1 cut(s) 1027
HpyAV CCTTC 6 cut(s) 794, 966, 1154, 1169, 1345, 1510
HpyCH4III ACNGT 4 cut(s) 295, 706, 1101, 1430
HpyCH4IV ACGT 1 cut(s) 789
HpyCH4V TGCA 6 cut(s) 686, 843, 1052, 1238, 1424, 1730
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 335, 1430
HpySE526I ACGT 1 cut(s) 789
Hsp92II CATG 6 cut(s) 186, 503, 857, 1052, 1172, 1253
HspAI GCGC 4 cut(s) 21, 343, 358, 679
Ksp22I TGATCA 3 cut(s) 165, 246, 1246
Kzo9I GATC 3 cut(s) 165, 246, 1246
LguI GCTCTTC 1 cut(s) 1284
LmnI GCTCC 3 cut(s) 958, 1201, 1436
Lsp1109I GCAGC 1 cut(s) 827
LweI GCATC 6 cut(s) 325, 1225, 1711, 1717, 1739, 1796
MaeI CTAG 6 cut(s) 27, 525, 656, 668, 1016, 1296
MaeII ACGT 1 cut(s) 789
MaeIII GTNAC 6 cut(s) 170, 295, 309, 482, 658, 823
MalI GATC 3 cut(s) 167, 248, 1248
MboI GATC 3 cut(s) 165, 246, 1246
MboII GAAGA 9 cut(s) 615, 845, 1301, 1583, 1610, 1697, 1772, 1805, 1808
MfeI CAATTG 2 cut(s) 303, 1658
MhlI GDGCHC 3 cut(s) 10, 232, 1394
MlyI GAGTC 4 cut(s) 200, 317, 1298, 1622
MmeI TCCRAC 2 cut(s) 133, 1006
Mph1103I ATGCAT 1 cut(s) 1732
MseI TTAA 4 cut(s) 452, 606, 965, 1362
MslI CAYNNNNRTG 1 cut(s) 181
MspI CCGG 2 cut(s) 330, 905
MspR9I CCNGG 1 cut(s) 906
MunI CAATTG 2 cut(s) 303, 1658
Mva1269I GAATGC 1 cut(s) 1186
MvnI CGCG 3 cut(s) 23, 345, 679
MwoI GCNNNNNNNGC 3 cut(s) 29, 335, 1430
NciI CCSGG 1 cut(s) 906
NdeI CATATG 1 cut(s) 383
NdeII GATC 3 cut(s) 165, 246, 1246
NlaIII CATG 6 cut(s) 186, 503, 857, 1052, 1172, 1253
NlaIV GGNNCC 5 cut(s) 42, 597, 721, 805, 903
NmuCI GTSAC 4 cut(s) 295, 309, 658, 823
NsiI ATGCAT 1 cut(s) 1732
NspI RCATGY 2 cut(s) 857, 1052
PaeI GCATGC 1 cut(s) 857
PagI TCATGA 1 cut(s) 1249
PaqCI CACCTGC 1 cut(s) 1481
PciSI GCTCTTC 1 cut(s) 1284
PctI GAATGC 1 cut(s) 1186
PfeI GAWTC 4 cut(s) 620, 1274, 1280, 1472
PkrI GCNGC 2 cut(s) 523, 842
PleI GAGTC 4 cut(s) 199, 316, 1298, 1621
PpsI GAGTC 4 cut(s) 199, 316, 1298, 1621
PpuMI RGGWCCY 1 cut(s) 40
Psp124BI GAGCTC 1 cut(s) 1394
Psp1406I AACGTT 1 cut(s) 789
Psp5II RGGWCCY 1 cut(s) 40
PspEI GGTNACC 1 cut(s) 295
PspN4I GGNNCC 5 cut(s) 42, 597, 721, 805, 903
PspPI GGNCC 5 cut(s) 40, 55, 695, 782, 1501
PspPPI RGGWCCY 1 cut(s) 40
PstNI CAGNNNCTG 1 cut(s) 1277
RseI CAYNNNNRTG 1 cut(s) 181
SacI GAGCTC 1 cut(s) 1394
SapI GCTCTTC 1 cut(s) 1284
SaqAI TTAA 4 cut(s) 452, 606, 965, 1362
SatI GCNGC 2 cut(s) 522, 841
Sau3AI GATC 3 cut(s) 165, 246, 1246
Sau96I GGNCC 5 cut(s) 40, 55, 695, 782, 1501
SchI GAGTC 4 cut(s) 200, 317, 1298, 1622
ScrFI CCNGG 1 cut(s) 906
SduI GDGCHC 3 cut(s) 10, 232, 1394
SfaNI GCATC 6 cut(s) 325, 1225, 1711, 1717, 1739, 1796
SfcI CTRYAG 1 cut(s) 858
SinI GGWCC 4 cut(s) 40, 695, 782, 1501
SmiMI CAYNNNNRTG 1 cut(s) 181
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
SphI GCATGC 1 cut(s) 857
SsiI CCGC 2 cut(s) 521, 1617
SspI AATATT 2 cut(s) 492, 1360
SspMI CTAG 6 cut(s) 27, 525, 656, 668, 1016, 1296
SstI GAGCTC 1 cut(s) 1394
StyD4I CCNGG 1 cut(s) 904
TaaI ACNGT 4 cut(s) 295, 706, 1101, 1430
TaiI ACGT 1 cut(s) 792
TaqI TCGA 4 cut(s) 378, 512, 1064, 1566
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 4 cut(s) 620, 1274, 1280, 1472
Tru1I TTAA 4 cut(s) 452, 606, 965, 1362
Tru9I TTAA 4 cut(s) 452, 606, 965, 1362
TscAI CASTG 4 cut(s) 1042, 1059, 1106, 1401
TseFI GTSAC 4 cut(s) 295, 309, 658, 823
TseI GCWGC 1 cut(s) 840
Tsp45I GTSAC 4 cut(s) 295, 309, 658, 823
TspRI CASTG 4 cut(s) 1042, 1059, 1106, 1401
VpaK11BI GGWCC 4 cut(s) 40, 695, 782, 1501
XapI RAATTY 7 cut(s) 104, 262, 504, 744, 751, 1592, 1740
XceI RCATGY 2 cut(s) 857, 1052
XspI CTAG 6 cut(s) 27, 525, 656, 668, 1016, 1296
Zsp2I ATGCAT 1 cut(s) 1732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.