Rmu_sc0001282.1_g000005

Responsible for synthesis of pseudouridine from uracil

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001282.1
OG0009164
Physical Location & Seq
Reverse (-)
31054 .. 34349
3296 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1242 bp
atgccaacgacgtcgtttgcgtctagggctctgcaaatttgcaaaaatctgatcccaaatcggaataaaagaggaaaaatgaagaggaagatagaagagacggacatggagatcgtctggcaatcgccggcgaaccctccggaacggaaagattacatctttcgcaacgggaggcgccatgtgagaccctactactttgagttcattgctcatgttaagaagcggtgggcggggaagaccgtcgttgacttgttcactgacgagttcaaaggccggaatcgtgactactatgtaggagtttctattagtgcggttaaatgtgggaggatacaagttgatgggcaacttgtgccggtttccttcatagttaaaccgtcgcaaaagataagccacttcctgcacaggcatgaaccgcccgtgatgacttgggatgtgaaaattcttgaaacagaacccgatgtggtcaccatttgtaaacctgcatctgttccagtgcatccatgtggtcagtatcgtaagaacactgttgttggcatcctacaggcagagcatggcttggcacctctatttccgattcatcgattagatcgtctggtctcaggactccttatcttggctagaagcgcagcacaagctgacattttcaggcaacagattgagggagggatggttcataaacagtacattgcaagagtcattggagagtttcctgaggaggagcaagttgtagatgctaacataaatcataatgctcgagaaggaaggagctctaccgaggtcggtgattcttgtggtgatgctcccacaaaggggaagactgcttgtacaaagtttactagaatcagtacgaatggaattcagagcattgtcttgtgtgaaccagtcactggccgcactcatcagatacgcgtccatttgcaacatgcaggccatccaatagcgaatgacatgctttacctgtccaaacatgtaagtgatcgttcagctgaaggaatgggtgctgatagagctgctgctaatccagaccactctccagcttctggtgttggcgaaaataaatccgcttcgcatgaagattctggtgaggacttcagcattgatccaatgtgtacaaattgtccaagtttagctccaaaaggatacgattatcatgaagaggccttgtggctacactgttttagatattctggacctggatgggtttatgagtgcccacatccggattgggcatcacttagctag

Protein Analysis

413

Amino Acids

46.25

Weight (kDa)

8.41

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 153 - 308 1.4e-28 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 487
AccB1I GGYRCC 2 cut(s) 174, 559
AccB7I CCANNNNNTGG 2 cut(s) 887, 1040
AccII CGCG 1 cut(s) 909
AccIII TCCGGA 2 cut(s) 139, 1219
AciI CCGC 6 cut(s) 223, 230, 311, 413, 892, 1062
AclWI GGATC 2 cut(s) 46, 1094
AcoI YGGCCR 1 cut(s) 889
AcsI RAATTY 3 cut(s) 36, 438, 855
AcuI CTGAAG 2 cut(s) 1008, 1075
AcyI GRCGYC 2 cut(s) 11, 175
AfaI GTAC 4 cut(s) 683, 826, 847, 1111
AfiI CCNNNNNNNGG 6 cut(s) 613, 809, 810, 887, 1040, 1219
AflIII ACRYGT 2 cut(s) 907, 967
AgsI TTSAA 2 cut(s) 268, 446
AjnI CCWGG 1 cut(s) 1192
AloI GAACNNNNNNTCC 2 cut(s) 654, 686
AluBI AGCT 7 cut(s) 635, 768, 986, 1010, 1037, 1130, 1239
AluI AGCT 7 cut(s) 635, 768, 986, 1010, 1037, 1130, 1239
Alw21I GWGCWC 1 cut(s) 770
Alw26I GTCTC 3 cut(s) 92, 178, 601
AlwI GGATC 2 cut(s) 46, 1094
AlwNI CAGNNNCTG 2 cut(s) 887, 1040
Ama87I CYCGRG 1 cut(s) 753
Aor13HI TCCGGA 2 cut(s) 139, 1219
AoxI GGCC 4 cut(s) 271, 889, 928, 1158
ApeKI GCWGC 3 cut(s) 626, 1010, 1013
ApoI RAATTY 3 cut(s) 36, 438, 855
AspLEI GCGC 2 cut(s) 177, 626
AspS9I GGNCC 1 cut(s) 1190
AsuHPI GGTGA 4 cut(s) 457, 794, 806, 1094
AvaI CYCGRG 1 cut(s) 753
AvaII GGWCC 1 cut(s) 1190
AxyI CCTNAGG 1 cut(s) 711
BaeGI GKGCMC 1 cut(s) 1214
BanI GGYRCC 2 cut(s) 174, 559
BanII GRGCYC 2 cut(s) 31, 770
BarI GAAGNNNNNNTAC 2 cut(s) 754, 786
BbsI GAAGAC 2 cut(s) 242, 821
Bbv12I GWGCWC 1 cut(s) 770
BbvI GCAGC 3 cut(s) 638, 997, 1000
BccI CCATC 4 cut(s) 332, 661, 939, 1191
BcgI CGANNNNNNTGC 2 cut(s) 931, 965
BciT130I CCWGG 1 cut(s) 1194
BciVI GTATCC 2 cut(s) 321, 1133
BcoDI GTCTC 3 cut(s) 92, 178, 601
BfaI CTAG 4 cut(s) 24, 618, 837, 1240
BfmI CTRYAG 1 cut(s) 539
BfoI RGCGCY 1 cut(s) 178
BfuAI ACCTGC 1 cut(s) 487
BfuI GTATCC 2 cut(s) 321, 1133
BisI GCNGC 4 cut(s) 627, 892, 1011, 1014
BlsI GCNGC 4 cut(s) 628, 893, 1012, 1015
Bme1390I CCNGG 1 cut(s) 1194
Bme18I GGWCC 1 cut(s) 1190
BmeT110I CYCGRG 1 cut(s) 753
BmgT120I GGNCC 1 cut(s) 1190
BmiI GGNNCC 2 cut(s) 176, 561
BmrFI CCNGG 1 cut(s) 1194
BmsI GCATC 6 cut(s) 491, 505, 543, 721, 787, 1238
BpiI GAAGAC 2 cut(s) 242, 821
BpmI CTGGAG 1 cut(s) 1017
Bsa29I ATCGAT 1 cut(s) 580
BsaHI GRCGYC 2 cut(s) 11, 175
BsaI GGTCTC 2 cut(s) 178, 601
BsaJI CCNNGG 1 cut(s) 774
BsaWI WCCGGW 2 cut(s) 139, 1219
BsaXI ACNNNNNCTCC 2 cut(s) 654, 684
Bsc4I CCNNNNNNNGG 6 cut(s) 613, 809, 810, 887, 1040, 1219
Bse118I RCCGGY 2 cut(s) 127, 352
Bse1I ACTGG 3 cut(s) 491, 881, 892
Bse21I CCTNAGG 1 cut(s) 711
Bse3DI GCAATG 2 cut(s) 204, 684
BseAI TCCGGA 2 cut(s) 139, 1219
BseBI CCWGG 1 cut(s) 1194
BseCI ATCGAT 1 cut(s) 580
BseDI CCNNGG 1 cut(s) 774
BseGI GGATG 7 cut(s) 436, 496, 534, 672, 931, 1202, 1216
BseLI CCNNNNNNNGG 6 cut(s) 613, 809, 810, 887, 1040, 1219
BseMI GCAATG 2 cut(s) 204, 684
BseMII CTCAG 2 cut(s) 612, 702
BseNI ACTGG 3 cut(s) 491, 881, 892
BseRI GAGGAG 2 cut(s) 728, 731
BseSI GKGCMC 1 cut(s) 1214
BseXI GCAGC 3 cut(s) 638, 997, 1000
BsgI GTGCAG 1 cut(s) 383
Bsh1236I CGCG 1 cut(s) 909
BshFI GGCC 4 cut(s) 273, 891, 930, 1160
BshNI GGYRCC 2 cut(s) 174, 559
BshVI ATCGAT 1 cut(s) 580
BsiHKAI GWGCWC 1 cut(s) 770
BsiHKCI CYCGRG 1 cut(s) 753
BsiSI CCGG 5 cut(s) 128, 140, 274, 353, 1220
BslI CCNNNNNNNGG 6 cut(s) 613, 809, 810, 887, 1040, 1219
BsmAI GTCTC 3 cut(s) 92, 178, 601
BsmBI CGTCTC 1 cut(s) 92
BsnI GGCC 4 cut(s) 273, 891, 930, 1160
Bso31I GGTCTC 2 cut(s) 178, 601
BsoBI CYCGRG 1 cut(s) 753
Bsp1286I GDGCHC 3 cut(s) 31, 770, 1214
Bsp13I TCCGGA 2 cut(s) 139, 1219
Bsp1407I TGTACA 2 cut(s) 824, 1109
Bsp143I GATC 5 cut(s) 51, 111, 586, 976, 1099
BspACI CCGC 6 cut(s) 223, 230, 311, 413, 892, 1062
BspANI GGCC 4 cut(s) 273, 891, 930, 1160
BspCNI CTCAG 2 cut(s) 611, 703
BspDI ATCGAT 1 cut(s) 580
BspEI TCCGGA 2 cut(s) 139, 1219
BspFNI CGCG 1 cut(s) 909
BspHI TCATGA 1 cut(s) 1150
BspLI GGNNCC 2 cut(s) 176, 561
BspMI ACCTGC 1 cut(s) 487
BspPI GGATC 2 cut(s) 46, 1094
BspT107I GGYRCC 2 cut(s) 174, 559
BspTNI GGTCTC 2 cut(s) 178, 601
BsrDI GCAATG 2 cut(s) 204, 684
BsrFI RCCGGY 2 cut(s) 127, 352
BsrGI TGTACA 2 cut(s) 824, 1109
BsrI ACTGG 3 cut(s) 491, 881, 892
BssAI RCCGGY 2 cut(s) 127, 352
BssECI CCNNGG 1 cut(s) 774
BssMI GATC 5 cut(s) 51, 111, 586, 976, 1099
BssNI GRCGYC 2 cut(s) 11, 175
Bst2UI CCWGG 1 cut(s) 1194
Bst4CI ACNGT 5 cut(s) 241, 375, 526, 681, 1175
Bst6I CTCTTC 3 cut(s) 77, 90, 1149
BstACI GRCGYC 2 cut(s) 11, 175
BstAPI GCANNNNNTGC 1 cut(s) 349
BstAUI TGTACA 2 cut(s) 824, 1109
BstC8I GCNNGC 2 cut(s) 129, 928
BstDEI CTNAG 3 cut(s) 598, 711, 1235
BstEII GGTNACC 1 cut(s) 463
BstF5I GGATG 7 cut(s) 436, 496, 534, 672, 931, 1202, 1216
BstFNI CGCG 1 cut(s) 909
BstH2I RGCGCY 1 cut(s) 178
BstHHI GCGC 2 cut(s) 177, 626
BstKTI GATC 5 cut(s) 54, 114, 589, 979, 1102
BstMAI GTCTC 3 cut(s) 92, 178, 601
BstMBI GATC 5 cut(s) 51, 111, 586, 976, 1099
BstMWI GCNNNNNNNGC 6 cut(s) 26, 349, 412, 623, 632, 1007
BstNI CCWGG 1 cut(s) 1194
BstNSI RCATGY 3 cut(s) 926, 952, 971
BstPI GGTNACC 1 cut(s) 463
BstSCI CCNGG 1 cut(s) 1192
BstSFI CTRYAG 1 cut(s) 539
BstSLI GKGCMC 1 cut(s) 1214
BstUI CGCG 1 cut(s) 909
BstV1I GCAGC 3 cut(s) 638, 997, 1000
BstV2I GAAGAC 2 cut(s) 242, 821
Bsu15I ATCGAT 1 cut(s) 580
Bsu36I CCTNAGG 1 cut(s) 711
BsuI GTATCC 2 cut(s) 321, 1133
BsuRI GGCC 4 cut(s) 273, 891, 930, 1160
BsuTUI ATCGAT 1 cut(s) 580
BtsCI GGATG 7 cut(s) 436, 496, 534, 672, 931, 1202, 1216
BtsIMutI CAGTG 5 cut(s) 255, 498, 522, 885, 1171
BveI ACCTGC 1 cut(s) 487
Cac8I GCNNGC 2 cut(s) 129, 928
CaiI CAGNNNCTG 2 cut(s) 887, 1040
CciI TCATGA 1 cut(s) 1150
CfoI GCGC 2 cut(s) 177, 626
Cfr10I RCCGGY 2 cut(s) 127, 352
Cfr13I GGNCC 1 cut(s) 1190
ClaI ATCGAT 1 cut(s) 580
CseI GACGC 2 cut(s) 9, 898
Csp6I GTAC 4 cut(s) 682, 825, 846, 1110
CviQI GTAC 4 cut(s) 682, 825, 846, 1110
DdeI CTNAG 3 cut(s) 598, 711, 1235
DinI GGCGCC 1 cut(s) 176
DpnI GATC 5 cut(s) 53, 113, 588, 978, 1101
DpnII GATC 5 cut(s) 51, 111, 586, 976, 1099
EaeI YGGCCR 1 cut(s) 889
Eam1104I CTCTTC 3 cut(s) 77, 90, 1149
EarI CTCTTC 3 cut(s) 77, 90, 1149
Ecl136II GAGCTC 1 cut(s) 768
Eco147I AGGCCT 1 cut(s) 1160
Eco24I GRGCYC 2 cut(s) 31, 770
Eco31I GGTCTC 2 cut(s) 178, 601
Eco47I GGWCC 1 cut(s) 1190
Eco53kI GAGCTC 1 cut(s) 768
Eco57I CTGAAG 2 cut(s) 1008, 1075
Eco81I CCTNAGG 1 cut(s) 711
Eco88I CYCGRG 1 cut(s) 753
Eco91I GGTNACC 1 cut(s) 463
EcoICRI GAGCTC 1 cut(s) 768
EcoO65I GGTNACC 1 cut(s) 463
EcoRI GAATTC 1 cut(s) 855
EcoRII CCWGG 1 cut(s) 1192
EcoT38I GRGCYC 2 cut(s) 31, 770
EgeI GGCGCC 1 cut(s) 176
EheI GGCGCC 1 cut(s) 176
Esp3I CGTCTC 1 cut(s) 92
FauI CCCGC 1 cut(s) 223
Fnu4HI GCNGC 4 cut(s) 627, 892, 1011, 1014
FokI GGATG 7 cut(s) 443, 483, 521, 679, 918, 1203, 1209
FriOI GRGCYC 2 cut(s) 31, 770
Fsp4HI GCNGC 4 cut(s) 627, 892, 1011, 1014
FspBI CTAG 4 cut(s) 24, 618, 837, 1240
GlaI GCGC 2 cut(s) 176, 625
GluI GCNGC 4 cut(s) 627, 892, 1011, 1014
GsuI CTGGAG 1 cut(s) 1017
HaeII RGCGCY 1 cut(s) 178
HaeIII GGCC 4 cut(s) 273, 891, 930, 1160
HapII CCGG 5 cut(s) 128, 140, 274, 353, 1220
HgaI GACGC 2 cut(s) 9, 898
HhaI GCGC 2 cut(s) 177, 626
Hin1I GRCGYC 2 cut(s) 11, 175
Hin6I GCGC 2 cut(s) 175, 624
HinP1I GCGC 2 cut(s) 175, 624
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 7 cut(s) 277, 574, 603, 693, 785, 840, 1076
HpaII CCGG 5 cut(s) 128, 140, 274, 353, 1220
HphI GGTGA 4 cut(s) 457, 794, 806, 1094
Hpy166II GTNNAC 6 cut(s) 247, 255, 476, 834, 878, 1110
Hpy188I TCNGA 5 cut(s) 51, 63, 573, 861, 903
Hpy8I GTNNAC 6 cut(s) 247, 255, 476, 834, 878, 1110
Hpy99I CGWCG 4 cut(s) 13, 16, 245, 379
HpyAV CCTTC 4 cut(s) 370, 752, 756, 983
HpyCH4III ACNGT 5 cut(s) 241, 375, 526, 681, 1175
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 8 cut(s) 34, 42, 400, 482, 496, 689, 919, 926
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 349, 412, 623, 632, 1007
HpyF3I CTNAG 3 cut(s) 598, 711, 1235
HpySE526I ACGT 1 cut(s) 11
Hsp92I GRCGYC 2 cut(s) 11, 175
HspAI GCGC 2 cut(s) 175, 624
KasI GGCGCC 1 cut(s) 174
Kpn2I TCCGGA 2 cut(s) 139, 1219
KroI GCCGGC 1 cut(s) 127
KroNI GCCGGC 1 cut(s) 129
Kzo9I GATC 5 cut(s) 51, 111, 586, 976, 1099
LmnI GCTCC 4 cut(s) 718, 765, 805, 1135
Lsp1109I GCAGC 3 cut(s) 638, 997, 1000
LweI GCATC 6 cut(s) 491, 505, 543, 721, 787, 1238
MaeI CTAG 4 cut(s) 24, 618, 837, 1240
MaeII ACGT 1 cut(s) 11
MaeIII GTNAC 3 cut(s) 281, 463, 883
MalI GATC 5 cut(s) 53, 113, 588, 978, 1101
MboI GATC 5 cut(s) 51, 111, 586, 976, 1099
MboII GAAGA 7 cut(s) 94, 100, 107, 247, 826, 1085, 1166
MhlI GDGCHC 3 cut(s) 31, 770, 1214
MluCI AATT 4 cut(s) 36, 438, 855, 1114
MluI ACGCGT 1 cut(s) 907
Mly113I GGCGCC 1 cut(s) 175
MlyI GAGTC 2 cut(s) 597, 702
MreI CGCCGGCG 1 cut(s) 127
MroI TCCGGA 2 cut(s) 139, 1219
MroNI GCCGGC 1 cut(s) 127
MseI TTAA 3 cut(s) 216, 315, 369
MslI CAYNNNNRTG 3 cut(s) 405, 501, 972
MspA1I CMGCKG 1 cut(s) 986
MspI CCGG 5 cut(s) 128, 140, 274, 353, 1220
MspR9I CCNGG 1 cut(s) 1194
MvaI CCWGG 1 cut(s) 1194
MvnI CGCG 1 cut(s) 909
MwoI GCNNNNNNNGC 6 cut(s) 26, 349, 412, 623, 632, 1007
NaeI GCCGGC 1 cut(s) 129
NarI GGCGCC 1 cut(s) 175
NdeII GATC 5 cut(s) 51, 111, 586, 976, 1099
NgoMIV GCCGGC 1 cut(s) 127
NlaIV GGNNCC 2 cut(s) 176, 561
NmuCI GTSAC 3 cut(s) 281, 463, 883
NspI RCATGY 3 cut(s) 926, 952, 971
PaeR7I CTCGAG 1 cut(s) 753
PagI TCATGA 1 cut(s) 1150
PceI AGGCCT 1 cut(s) 1160
PciI ACATGT 1 cut(s) 967
PcsI WCGNNNNNNNCGW 2 cut(s) 17, 586
PdiI GCCGGC 1 cut(s) 129
PfeI GAWTC 5 cut(s) 277, 574, 785, 840, 1076
PflMI CCANNNNNTGG 2 cut(s) 887, 1040
PkrI GCNGC 4 cut(s) 628, 893, 1012, 1015
PleI GAGTC 2 cut(s) 597, 701
PluTI GGCGCC 1 cut(s) 178
PpsI GAGTC 2 cut(s) 597, 701
PscI ACATGT 1 cut(s) 967
Psp124BI GAGCTC 1 cut(s) 770
Psp6I CCWGG 1 cut(s) 1192
PspEI GGTNACC 1 cut(s) 463
PspGI CCWGG 1 cut(s) 1192
PspN4I GGNNCC 2 cut(s) 176, 561
PspPI GGNCC 1 cut(s) 1190
PstNI CAGNNNCTG 2 cut(s) 887, 1040
PvuII CAGCTG 1 cut(s) 986
RsaI GTAC 4 cut(s) 683, 826, 847, 1111
RsaNI GTAC 4 cut(s) 682, 825, 846, 1110
RseI CAYNNNNRTG 3 cut(s) 405, 501, 972
SacI GAGCTC 1 cut(s) 770
SaqAI TTAA 3 cut(s) 216, 315, 369
SatI GCNGC 4 cut(s) 627, 892, 1011, 1014
Sau3AI GATC 5 cut(s) 51, 111, 586, 976, 1099
Sau96I GGNCC 1 cut(s) 1190
SchI GAGTC 2 cut(s) 597, 702
ScrFI CCNGG 1 cut(s) 1194
SduI GDGCHC 3 cut(s) 31, 770, 1214
SfaNI GCATC 6 cut(s) 491, 505, 543, 721, 787, 1238
SfcI CTRYAG 1 cut(s) 539
SfoI GGCGCC 1 cut(s) 176
Sfr274I CTCGAG 1 cut(s) 753
SgrAI CRCCGGYG 1 cut(s) 127
SinI GGWCC 1 cut(s) 1190
SlaI CTCGAG 1 cut(s) 753
SmiMI CAYNNNNRTG 3 cut(s) 405, 501, 972
SmlI CTYRAG 1 cut(s) 753
SmoI CTYRAG 1 cut(s) 753
Sse9I AATT 4 cut(s) 36, 438, 855, 1114
SseBI AGGCCT 1 cut(s) 1160
SsiI CCGC 6 cut(s) 223, 230, 311, 413, 892, 1062
SspDI GGCGCC 1 cut(s) 174
SspMI CTAG 4 cut(s) 24, 618, 837, 1240
SstI GAGCTC 1 cut(s) 770
StuI AGGCCT 1 cut(s) 1160
StyD4I CCNGG 1 cut(s) 1192
TaaI ACNGT 5 cut(s) 241, 375, 526, 681, 1175
TaiI ACGT 1 cut(s) 14
TaqI TCGA 2 cut(s) 580, 754
TasI AATT 4 cut(s) 36, 438, 855, 1114
TatI WGTACW 3 cut(s) 681, 824, 1109
TauI GCSGC 1 cut(s) 894
TfiI GAWTC 5 cut(s) 277, 574, 785, 840, 1076
Tru1I TTAA 3 cut(s) 216, 315, 369
Tru9I TTAA 3 cut(s) 216, 315, 369
TscAI CASTG 5 cut(s) 262, 498, 529, 892, 1178
TseFI GTSAC 3 cut(s) 281, 463, 883
TseI GCWGC 3 cut(s) 626, 1010, 1013
Tsp45I GTSAC 3 cut(s) 281, 463, 883
TspDTI ATGAA 8 cut(s) 95, 193, 352, 423, 566, 662, 1086, 1167
TspGWI ACGGA 2 cut(s) 116, 160
TspRI CASTG 5 cut(s) 262, 498, 529, 892, 1178
Van91I CCANNNNNTGG 2 cut(s) 887, 1040
VpaK11BI GGWCC 1 cut(s) 1190
XapI RAATTY 3 cut(s) 36, 438, 855
XceI RCATGY 3 cut(s) 926, 952, 971
XcmI CCANNNNNNNNNTGG 1 cut(s) 1221
XhoI CTCGAG 1 cut(s) 753
XspI CTAG 4 cut(s) 24, 618, 837, 1240
ZraI GACGTC 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.