Rmu_sc0001354.1_g000029

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001354.1
OG0011526
Physical Location & Seq
Reverse (-)
117278 .. 118420
1143 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1143 bp
atgggtctgaaatcgatgaacattttggcaatacttttgctctcatttttagcatccaccgctgtagctgatcaatccaatgtcttcgatgtgacaagctcaaaatatggtggaaagcccaactcggatatttcaaaggcattgacaatggcatggacagatgcgtgtgcatcaacgtcaacgagtactgtccttgttccgaggggaacatgcaagtttttacaagcaaaatttgaaggtccttgcaaggctcctattcagtttcagctccaaggaacattgcaggctccgaaagaccggagccaattcgatgactcttggatttcctttaacaacatcgacatgctcaccttgtccggcggtggaactatcgacagacaaggagccctttcttggaaggagaatcatgactctactcccgttgatatttggttccaatctgtcaacaattccacaattagcgacatagcttcaatcgacagcgcaaagttccacattagtgttcatggctgcaacaacgttacgttccaacggctcaccatcacagcacccgaagacagtcctaacaccgacggaatccacatctctagttcgaccaagatcacagtctccgactcctacattgcaaccggtgacgattgtgtttcaattggcgatggctccaaccaaatcctagtgtccaacattacttgcggaccaggtcatggaataagcataggcagtctaggaaagtatccagacgaacagccggtggtcggcgtcacggtcaagaactgcacgcttaccggcacaagcaacggcgcgagaatcaagacatggcccgattctccggcaacaagcgttgcttgtaatatactcttccaagacatggtcatggtgaatgtgaagaaccctatccttatcgaccagaactactgtgacaaaggcaagtcgtgtaagcaaataattccatccaaagtccagatcagtaacgtgacgttcaaggatatcaggggctcgtcttcgtctccggttgcagtcaagctcgactgtagtcacagcgcgccgtgccggaatgtggagctgtccgatattgatctcacgtacagtggagacaaaaaagacgaaatcacgtctgagtgtaataatgtcaaacctgtttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

380

Amino Acids

40.61

Weight (kDa)

5.51

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 56 - 378 1.6e-97 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 704, 868
AccII CGCG 2 cut(s) 803, 1043
AciI CCGC 3 cut(s) 60, 360, 693
AclI AACGTT 1 cut(s) 519
AcsI RAATTY 1 cut(s) 230
AcyI GRCGYC 1 cut(s) 759
AfaI GTAC 2 cut(s) 187, 1085
AfiI CCNNNNNNNGG 5 cut(s) 393, 704, 755, 868, 1057
AgeI ACCGGT 1 cut(s) 629
AgsI TTSAA 5 cut(s) 135, 236, 474, 648, 982
AjiI CACGTC 1 cut(s) 1113
AjnI CCWGG 1 cut(s) 697
AleI CACNNNNGTG 1 cut(s) 498
AluBI AGCT 6 cut(s) 68, 99, 268, 470, 1024, 1063
AluI AGCT 6 cut(s) 68, 99, 268, 470, 1024, 1063
Alw26I GTCTC 3 cut(s) 613, 1011, 1086
AoxI GGCC 1 cut(s) 818
ApeKI GCWGC 1 cut(s) 510
ApoI RAATTY 1 cut(s) 230
AsiGI ACCGGT 1 cut(s) 629
AspLEI GCGC 4 cut(s) 485, 803, 1043, 1045
AspS9I GGNCC 3 cut(s) 239, 695, 819
AsuHPI GGTGA 4 cut(s) 340, 529, 644, 889
AvaII GGWCC 2 cut(s) 239, 695
BanII GRGCYC 2 cut(s) 388, 998
BbsI GAAGAC 3 cut(s) 76, 561, 993
BbvI GCAGC 1 cut(s) 497
BccI CCATC 3 cut(s) 548, 650, 958
BceAI ACGGC 3 cut(s) 548, 814, 1030
BciT130I CCWGG 1 cut(s) 699
BciVI GTATCC 1 cut(s) 744
BclI TGATCA 1 cut(s) 70
BcoDI GTCTC 3 cut(s) 613, 1011, 1086
BfaI CTAG 3 cut(s) 588, 674, 725
BfmI CTRYAG 2 cut(s) 63, 1030
BfuI GTATCC 1 cut(s) 744
BisI GCNGC 1 cut(s) 511
BlsI GCNGC 1 cut(s) 512
BmcAI AGTACT 1 cut(s) 187
Bme1390I CCNGG 1 cut(s) 699
Bme18I GGWCC 2 cut(s) 239, 695
BmgBI CACGTC 1 cut(s) 1113
BmgT120I GGNCC 3 cut(s) 239, 695, 819
BmiI GGNNCC 6 cut(s) 252, 288, 302, 385, 434, 661
BmrFI CCNGG 1 cut(s) 699
BmsI GCATC 3 cut(s) 62, 151, 179
BoxI GACNNNNGTC 1 cut(s) 1032
BpiI GAAGAC 3 cut(s) 76, 561, 993
Bsa29I ATCGAT 1 cut(s) 14
BsaAI YACGTR 1 cut(s) 1083
BsaBI GATNNNNATC 1 cut(s) 1074
BsaHI GRCGYC 1 cut(s) 759
BsaJI CCNNGG 2 cut(s) 200, 271
BsaWI WCCGGW 3 cut(s) 297, 629, 1009
Bsc4I CCNNNNNNNGG 5 cut(s) 393, 704, 755, 868, 1057
Bse118I RCCGGY 3 cut(s) 629, 748, 785
Bse3DI GCAATG 2 cut(s) 278, 621
Bse8I GATNNNNATC 1 cut(s) 1074
BseBI CCWGG 1 cut(s) 699
BseCI ATCGAT 1 cut(s) 14
BseDI CCNNGG 2 cut(s) 200, 271
BseGI GGATG 2 cut(s) 53, 950
BseJI GATNNNNATC 1 cut(s) 1074
BseLI CCNNNNNNNGG 5 cut(s) 393, 704, 755, 868, 1057
BseMI GCAATG 2 cut(s) 278, 621
BseMII CTCAG 1 cut(s) 1107
BsePI GCGCGC 1 cut(s) 1041
BseXI GCAGC 1 cut(s) 497
BsgI GTGCAG 1 cut(s) 760
Bsh1236I CGCG 2 cut(s) 803, 1043
BshFI GGCC 1 cut(s) 820
BshTI ACCGGT 1 cut(s) 629
BshVI ATCGAT 1 cut(s) 14
BsiSI CCGG 8 cut(s) 298, 357, 630, 749, 786, 830, 1010, 1051
BslI CCNNNNNNNGG 5 cut(s) 393, 704, 755, 868, 1057
BsmAI GTCTC 3 cut(s) 613, 1011, 1086
BsmBI CGTCTC 1 cut(s) 1011
BsnI GGCC 1 cut(s) 820
Bsp1286I GDGCHC 2 cut(s) 388, 998
Bsp143I GATC 4 cut(s) 70, 600, 963, 1075
BspACI CCGC 3 cut(s) 60, 360, 693
BspANI GGCC 1 cut(s) 820
BspCNI CTCAG 1 cut(s) 1108
BspDI ATCGAT 1 cut(s) 14
BspFNI CGCG 2 cut(s) 803, 1043
BspHI TCATGA 1 cut(s) 406
BspLI GGNNCC 6 cut(s) 252, 288, 302, 385, 434, 661
BsrDI GCAATG 2 cut(s) 278, 621
BsrFI RCCGGY 3 cut(s) 629, 748, 785
BssAI RCCGGY 3 cut(s) 629, 748, 785
BssECI CCNNGG 2 cut(s) 200, 271
BssHII GCGCGC 1 cut(s) 1041
BssMI GATC 4 cut(s) 70, 600, 963, 1075
BssNI GRCGYC 1 cut(s) 759
BssT1I CCWWGG 1 cut(s) 271
Bst2UI CCWGG 1 cut(s) 699
Bst4CI ACNGT 7 cut(s) 190, 560, 607, 766, 917, 1031, 1088
Bst6I CTCTTC 1 cut(s) 863
BstACI GRCGYC 1 cut(s) 759
BstBAI YACGTR 1 cut(s) 1083
BstC8I GCNNGC 3 cut(s) 285, 779, 1043
BstDEI CTNAG 1 cut(s) 1116
BstF5I GGATG 2 cut(s) 53, 950
BstFNI CGCG 2 cut(s) 803, 1043
BstHHI GCGC 4 cut(s) 485, 803, 1043, 1045
BstKTI GATC 4 cut(s) 73, 603, 966, 1078
BstMAI GTCTC 3 cut(s) 613, 1011, 1086
BstMBI GATC 4 cut(s) 70, 600, 963, 1075
BstMWI GCNNNNNNNGC 2 cut(s) 59, 1047
BstNI CCWGG 1 cut(s) 699
BstNSI RCATGY 2 cut(s) 213, 346
BstPAI GACNNNNGTC 1 cut(s) 1032
BstSCI CCNGG 1 cut(s) 697
BstSFI CTRYAG 2 cut(s) 63, 1030
BstUI CGCG 2 cut(s) 803, 1043
BstV1I GCAGC 1 cut(s) 497
BstV2I GAAGAC 3 cut(s) 76, 561, 993
Bsu15I ATCGAT 1 cut(s) 14
BsuI GTATCC 1 cut(s) 744
BsuRI GGCC 1 cut(s) 820
BsuTUI ATCGAT 1 cut(s) 14
BtgZI GCGATG 1 cut(s) 669
BtrI CACGTC 1 cut(s) 1113
BtsCI GGATG 2 cut(s) 53, 950
BtsIMutI CAGTG 1 cut(s) 1093
Cac8I GCNNGC 3 cut(s) 285, 779, 1043
CciI TCATGA 1 cut(s) 406
CfoI GCGC 4 cut(s) 485, 803, 1043, 1045
Cfr10I RCCGGY 3 cut(s) 629, 748, 785
Cfr13I GGNCC 3 cut(s) 239, 695, 819
ClaI ATCGAT 1 cut(s) 14
CseI GACGC 1 cut(s) 748
CsiI ACCWGGT 1 cut(s) 697
Csp6I GTAC 2 cut(s) 186, 1084
CspAI ACCGGT 1 cut(s) 629
CviAII CATG 9 cut(s) 153, 210, 343, 407, 506, 704, 816, 868, 874
CviQI GTAC 2 cut(s) 186, 1084
DdeI CTNAG 1 cut(s) 1116
DpnI GATC 4 cut(s) 72, 602, 965, 1077
DpnII GATC 4 cut(s) 70, 600, 963, 1075
Eam1104I CTCTTC 1 cut(s) 863
EarI CTCTTC 1 cut(s) 863
Eco130I CCWWGG 1 cut(s) 271
Eco24I GRGCYC 2 cut(s) 388, 998
Eco32I GATATC 1 cut(s) 988
Eco47I GGWCC 2 cut(s) 239, 695
EcoO109I RGGNCCY 1 cut(s) 239
EcoRII CCWGG 1 cut(s) 697
EcoRV GATATC 1 cut(s) 988
EcoT14I CCWWGG 1 cut(s) 271
EcoT38I GRGCYC 2 cut(s) 388, 998
ErhI CCWWGG 1 cut(s) 271
Esp3I CGTCTC 1 cut(s) 1011
FaeI CATG 9 cut(s) 156, 213, 346, 410, 509, 707, 819, 871, 877
FalI AAGNNNNNCTT 4 cut(s) 372, 404, 829, 861
FatI CATG 9 cut(s) 152, 209, 342, 406, 505, 703, 815, 867, 873
FbaI TGATCA 1 cut(s) 70
Fnu4HI GCNGC 1 cut(s) 511
FokI GGATG 2 cut(s) 40, 937
FriOI GRGCYC 2 cut(s) 388, 998
Fsp4HI GCNGC 1 cut(s) 511
FspBI CTAG 3 cut(s) 588, 674, 725
GlaI GCGC 4 cut(s) 484, 802, 1042, 1044
GluI GCNGC 1 cut(s) 511
HaeIII GGCC 1 cut(s) 820
HapII CCGG 8 cut(s) 298, 357, 630, 749, 786, 830, 1010, 1051
HgaI GACGC 1 cut(s) 748
HhaI GCGC 4 cut(s) 485, 803, 1043, 1045
Hin1I GRCGYC 1 cut(s) 759
Hin1II CATG 9 cut(s) 156, 213, 346, 410, 509, 707, 819, 871, 877
Hin6I GCGC 4 cut(s) 483, 801, 1041, 1043
HinP1I GCGC 4 cut(s) 483, 801, 1041, 1043
HincII GTYRAC 2 cut(s) 180, 445
HindII GTYRAC 2 cut(s) 180, 445
HinfI GANTC 7 cut(s) 314, 403, 410, 576, 614, 807, 824
HpaII CCGG 8 cut(s) 298, 357, 630, 749, 786, 830, 1010, 1051
HphI GGTGA 4 cut(s) 340, 529, 644, 889
Hpy166II GTNNAC 2 cut(s) 180, 445
Hpy188I TCNGA 7 cut(s) 9, 127, 201, 291, 613, 1069, 1117
Hpy188III TCNNGA 5 cut(s) 407, 737, 769, 811, 961
Hpy8I GTNNAC 2 cut(s) 180, 445
Hpy99I CGWCG 1 cut(s) 575
HpyAV CCTTC 2 cut(s) 230, 391
HpyCH4III ACNGT 7 cut(s) 190, 560, 607, 766, 917, 1031, 1088
HpyCH4IV ACGT 7 cut(s) 176, 519, 524, 972, 977, 1082, 1112
HpyCH4V TGCA 8 cut(s) 170, 213, 246, 283, 513, 626, 777, 1016
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 1047
HpyF3I CTNAG 1 cut(s) 1116
HpySE526I ACGT 7 cut(s) 176, 519, 524, 972, 977, 1082, 1112
Hsp92I GRCGYC 1 cut(s) 759
Hsp92II CATG 9 cut(s) 156, 213, 346, 410, 509, 707, 819, 871, 877
HspAI GCGC 4 cut(s) 483, 801, 1041, 1043
Ksp22I TGATCA 1 cut(s) 70
Kzo9I GATC 4 cut(s) 70, 600, 963, 1075
LmnI GCTCC 7 cut(s) 256, 273, 292, 300, 383, 665, 1060
Lsp1109I GCAGC 1 cut(s) 497
LweI GCATC 3 cut(s) 62, 151, 179
MabI ACCWGGT 1 cut(s) 697
MaeI CTAG 3 cut(s) 588, 674, 725
MaeII ACGT 7 cut(s) 176, 519, 524, 972, 977, 1082, 1112
MaeIII GTNAC 8 cut(s) 91, 520, 632, 760, 917, 968, 973, 1034
MalI GATC 4 cut(s) 72, 602, 965, 1077
MboI GATC 4 cut(s) 70, 600, 963, 1075
MboII GAAGA 5 cut(s) 76, 566, 850, 898, 993
MfeI CAATTG 1 cut(s) 648
MhlI GDGCHC 2 cut(s) 388, 998
MluCI AATT 6 cut(s) 230, 305, 448, 456, 648, 945
MlyI GAGTC 3 cut(s) 308, 404, 608
MmeI TCCRAC 4 cut(s) 553, 636, 687, 705
MnlI CCTC 1 cut(s) 195
MseI TTAA 2 cut(s) 330, 1141
MslI CAYNNNNRTG 3 cut(s) 341, 498, 872
MspA1I CMGCKG 1 cut(s) 62
MspI CCGG 8 cut(s) 298, 357, 630, 749, 786, 830, 1010, 1051
MspR9I CCNGG 1 cut(s) 699
MunI CAATTG 1 cut(s) 648
MvaI CCWGG 1 cut(s) 699
MvnI CGCG 2 cut(s) 803, 1043
MwoI GCNNNNNNNGC 2 cut(s) 59, 1047
NdeII GATC 4 cut(s) 70, 600, 963, 1075
NlaIII CATG 9 cut(s) 156, 213, 346, 410, 509, 707, 819, 871, 877
NlaIV GGNNCC 6 cut(s) 252, 288, 302, 385, 434, 661
NmuCI GTSAC 6 cut(s) 91, 632, 760, 917, 973, 1034
NspI RCATGY 2 cut(s) 213, 346
OliI CACNNNNGTG 1 cut(s) 498
PagI TCATGA 1 cut(s) 406
PauI GCGCGC 1 cut(s) 1041
PfeI GAWTC 4 cut(s) 403, 576, 807, 824
PflFI GACNNNGTC 2 cut(s) 699, 869
PflMI CCANNNNNTGG 2 cut(s) 704, 868
PinAI ACCGGT 1 cut(s) 629
PkrI GCNGC 1 cut(s) 512
PleI GAGTC 3 cut(s) 308, 404, 608
PpsI GAGTC 3 cut(s) 308, 404, 608
Ppu21I YACGTR 1 cut(s) 1083
PpuMI RGGWCCY 1 cut(s) 239
PshAI GACNNNNGTC 1 cut(s) 1032
Psp1406I AACGTT 1 cut(s) 519
Psp5II RGGWCCY 1 cut(s) 239
Psp6I CCWGG 1 cut(s) 697
PspGI CCWGG 1 cut(s) 697
PspN4I GGNNCC 6 cut(s) 252, 288, 302, 385, 434, 661
PspPI GGNCC 3 cut(s) 239, 695, 819
PspPPI RGGWCCY 1 cut(s) 239
PsyI GACNNNGTC 2 cut(s) 699, 869
PteI GCGCGC 1 cut(s) 1041
RsaI GTAC 2 cut(s) 187, 1085
RsaNI GTAC 2 cut(s) 186, 1084
RseI CAYNNNNRTG 3 cut(s) 341, 498, 872
SaqAI TTAA 2 cut(s) 330, 1141
SatI GCNGC 1 cut(s) 511
Sau3AI GATC 4 cut(s) 70, 600, 963, 1075
Sau96I GGNCC 3 cut(s) 239, 695, 819
ScaI AGTACT 1 cut(s) 187
SchI GAGTC 3 cut(s) 308, 404, 608
ScrFI CCNGG 1 cut(s) 699
SduI GDGCHC 2 cut(s) 388, 998
SexAI ACCWGGT 1 cut(s) 697
SfaNI GCATC 3 cut(s) 62, 151, 179
SfcI CTRYAG 2 cut(s) 63, 1030
SinI GGWCC 2 cut(s) 239, 695
SmiMI CAYNNNNRTG 3 cut(s) 341, 498, 872
Sse9I AATT 6 cut(s) 230, 305, 448, 456, 648, 945
SsiI CCGC 3 cut(s) 60, 360, 693
SspMI CTAG 3 cut(s) 588, 674, 725
StyD4I CCNGG 1 cut(s) 697
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 7 cut(s) 190, 560, 607, 766, 917, 1031, 1088
TaiI ACGT 7 cut(s) 179, 522, 527, 975, 980, 1085, 1115
TaqI TCGA 9 cut(s) 14, 87, 309, 339, 372, 477, 593, 903, 1026
TasI AATT 6 cut(s) 230, 305, 448, 456, 648, 945
TatI WGTACW 1 cut(s) 185
TfiI GAWTC 4 cut(s) 403, 576, 807, 824
Tru1I TTAA 2 cut(s) 330, 1141
Tru9I TTAA 2 cut(s) 330, 1141
TscAI CASTG 1 cut(s) 1093
TseFI GTSAC 6 cut(s) 91, 632, 760, 917, 973, 1034
TseI GCWGC 1 cut(s) 510
Tsp45I GTSAC 6 cut(s) 91, 632, 760, 917, 973, 1034
TspDTI ATGAA 2 cut(s) 32, 494
TspGWI ACGGA 1 cut(s) 588
TspRI CASTG 1 cut(s) 1093
Tth111I GACNNNGTC 2 cut(s) 699, 869
Van91I CCANNNNNTGG 2 cut(s) 704, 868
VpaK11BI GGWCC 2 cut(s) 239, 695
XapI RAATTY 1 cut(s) 230
XceI RCATGY 2 cut(s) 213, 346
XspI CTAG 3 cut(s) 588, 674, 725
ZrmI AGTACT 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.