Rmu_sc0001527.1_g000012

Zeta toxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001527.1
OG0007046
Physical Location & Seq
Forward (+)
53531 .. 56921
3391 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1713 bp
atgttaccagacttacagtttgcttggaggaacttctcaagtggggtttggattggaactctaggaattcggttttttggtgaagttttgggatttgaagaagtgggtatagcggtattactactagcttggtttgtgaagaaagatgaaagcttggttgagctgtgggaatttgaagatgtgggtctttctgggttcgtggataaaggtggtaacttgcattttgaagcagcagttcttgcagaggatgacatggtagtagcacctacaaggaccaaactcagtttttgcctgtttatgccgctacaacttttccttttggtttcgccttcatgcaaaaagctatgtaatttgtcattcctcacctctcaattttcttcagaagaaaacaacggcaaaaccaccttcgcacatatcctatcagccgctgccatcggcatcatcattgccgcggcggtgcattatcgtcaacgaaagacaaagtatgagaagatcatcccacgcttaagggtctcggactccagccgcgttcataagatcgaaaggttccctcattatgtagctaggcagctgggatttaaagatagaagagaatgtccacacctctgcaaattggccaccgaatacatgagaaaatgcgacgggtttgaggatgatatctacagtttcttttctagtgaaccggatgcggactcgctgtttgtgaagcttctggaggagtttgagagatgcatcctcagttatttcgcatttcattggaacgaagctgatctgatgattagtcaggtcttgagttgtgatactgagccgaggaagaagctcaagaacatcgtcatggcagcgaccagggaacagagatttgaaagggtgaccaaaaatttgaaggtggctagagttttcacaacgttactggaggagatgaaagcaatgggactggccacagcggatgactcaacatgcacagaggtgatggctccggtggctcatgctgatagaagtccagtcctcctctttatgggaggtggaatgggagcagggaagagcaccgtgctaaaggacattctcaaagagccattctgggcaggagcagcagggaatgcagtagtgattgaagcagatgccttcaaagaatcggatgtcatatacagagctctcagctccagagggcatcacgacatgcttcaaacagctgaactggtacaccaatcatctacagacgcagcatcatcccttctggtcacagcacttaacgaagggagagatgtgatcatggatggcacactctcttgggtaccatttgttgttcagactgtaacaatggccagaaatgtccaccgccgccgttatcgcatgggagctggttacagggtgaatgacgatggaactgtcactgaaaactattgggaacgaattgaagatgaagaaccagaacaagtagaagggggcagaaagagaaaaccatacaggatagagcttgttggagttgtgtgtgatgcttatctggctgttataagaggaataaggcgagctatcatgtgtagaagagctgttcgagtaaacgcacaattgaaatcccacaagagattttcagaggcatttaaaacatattgccaactagttgacaatgctagactgtatagcactaatacgttggaaggaccacctaaggtacacctgctatcccttctcttagtttatcttcctttccggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zeta_toxin

Protein Analysis

570

Amino Acids

64.5

Weight (kDa)

7.57

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zeta_toxin PF06414 329 - 432 2.9e-14 Zeta toxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1511
AarI CACCTGC 1 cut(s) 1683
Acc36I ACCTGC 1 cut(s) 1683
Acc65I GGTACC 1 cut(s) 1291
AccB1I GGYRCC 1 cut(s) 1291
AccII CGCG 2 cut(s) 452, 528
AclI AACGTT 1 cut(s) 905
AcoI YGGCCR 3 cut(s) 615, 936, 1320
AcsI RAATTY 3 cut(s) 66, 170, 877
AcuI CTGAAG 1 cut(s) 363
AfaI GTAC 3 cut(s) 1200, 1293, 1671
AfiI CCNNNNNNNGG 1 cut(s) 1015
AflII CTTAAG 1 cut(s) 505
AhlI ACTAGT 1 cut(s) 1615
AjnI CCWGG 1 cut(s) 845
AleI CACNNNNGTG 1 cut(s) 965
Alw21I GWGCWC 2 cut(s) 1046, 1153
Alw26I GTCTC 1 cut(s) 517
AlwNI CAGNNNCTG 1 cut(s) 428
AoxI GGCC 3 cut(s) 615, 936, 1320
ApeKI GCWGC 6 cut(s) 230, 428, 568, 839, 1088, 1220
ApoI RAATTY 3 cut(s) 66, 170, 877
ArsI GACNNNNNNTTYG 5 cut(s) 34, 683, 715, 862, 894
Asp718I GGTACC 1 cut(s) 1291
AspS9I GGNCC 2 cut(s) 273, 1658
AsuHPI GGTGA 5 cut(s) 92, 355, 880, 979, 1381
AvaII GGWCC 2 cut(s) 273, 1658
AxyI CCTNAGG 1 cut(s) 1665
BalI TGGCCA 3 cut(s) 617, 938, 1322
BanI GGYRCC 1 cut(s) 1291
BanII GRGCYC 1 cut(s) 1153
Bbv12I GWGCWC 2 cut(s) 1046, 1153
BbvI GCAGC 6 cut(s) 242, 415, 580, 851, 1100, 1232
BccI CCATC 4 cut(s) 440, 964, 1268, 1373
BceAI ACGGC 2 cut(s) 409, 1326
BciT130I CCWGG 1 cut(s) 847
BclI TGATCA 1 cut(s) 1266
BcoDI GTCTC 1 cut(s) 517
BcuI ACTAGT 1 cut(s) 1615
BfaI CTAG 7 cut(s) 62, 125, 564, 675, 891, 1616, 1629
BfmI CTRYAG 2 cut(s) 661, 1212
BfrI CTTAAG 1 cut(s) 505
BfuAI ACCTGC 1 cut(s) 1683
Bme1390I CCNGG 1 cut(s) 847
Bme18I GGWCC 2 cut(s) 273, 1658
BmgT120I GGNCC 2 cut(s) 273, 1658
BmiI GGNNCC 3 cut(s) 548, 975, 1293
BmrFI CCNGG 1 cut(s) 847
BmsI GCATC 8 cut(s) 447, 676, 719, 741, 1108, 1177, 1232, 1483
BpmI CTGGAG 4 cut(s) 505, 734, 932, 1144
BpuEI CTTGAG 3 cut(s) 22, 806, 811
BsaBI GATNNNNATC 1 cut(s) 1497
BsaI GGTCTC 1 cut(s) 517
BsaJI CCNNGG 3 cut(s) 450, 809, 846
BsaWI WCCGGW 3 cut(s) 682, 976, 1707
Bsc4I CCNNNNNNNGG 1 cut(s) 1015
Bse1I ACTGG 4 cut(s) 915, 939, 1001, 1200
Bse21I CCTNAGG 1 cut(s) 1665
Bse3DI GCAATG 2 cut(s) 444, 933
Bse8I GATNNNNATC 1 cut(s) 1497
BseBI CCWGG 1 cut(s) 847
BseDI CCNNGG 3 cut(s) 450, 809, 846
BseGI GGATG 9 cut(s) 253, 495, 658, 691, 732, 952, 1141, 1226, 1279
BseJI GATNNNNATC 1 cut(s) 1497
BseLI CCNNNNNNNGG 1 cut(s) 1015
BseMI GCAATG 2 cut(s) 444, 933
BseMII CTCAG 4 cut(s) 295, 751, 795, 1168
BseNI ACTGG 4 cut(s) 915, 939, 1001, 1200
BseRI GAGGAG 3 cut(s) 731, 929, 998
BseXI GCAGC 6 cut(s) 242, 415, 580, 851, 1100, 1232
BseYI CCCAGC 1 cut(s) 571
Bsh1236I CGCG 2 cut(s) 452, 528
BshFI GGCC 3 cut(s) 617, 938, 1322
BshNI GGYRCC 1 cut(s) 1291
BsiHKAI GWGCWC 2 cut(s) 1046, 1153
BsiSI CCGG 3 cut(s) 683, 977, 1708
BslFI GGGAC 1 cut(s) 945
BslI CCNNNNNNNGG 1 cut(s) 1015
BsmAI GTCTC 1 cut(s) 517
BsmFI GGGAC 1 cut(s) 945
BsmI GAATGC 1 cut(s) 1102
BsnI GGCC 3 cut(s) 617, 938, 1322
Bso31I GGTCTC 1 cut(s) 517
Bsp1286I GDGCHC 2 cut(s) 1046, 1153
Bsp143I GATC 4 cut(s) 492, 537, 769, 1266
BspANI GGCC 3 cut(s) 617, 938, 1322
BspCNI CTCAG 4 cut(s) 294, 750, 796, 1167
BspFNI CGCG 2 cut(s) 452, 528
BspLI GGNNCC 3 cut(s) 548, 975, 1293
BspMI ACCTGC 1 cut(s) 1683
BspQI GCTCTTC 2 cut(s) 1034, 1537
BspT107I GGYRCC 1 cut(s) 1291
BspTI CTTAAG 1 cut(s) 505
BspTNI GGTCTC 1 cut(s) 517
BsrDI GCAATG 2 cut(s) 444, 933
BsrI ACTGG 4 cut(s) 915, 939, 1001, 1200
BssECI CCNNGG 3 cut(s) 450, 809, 846
BssMI GATC 4 cut(s) 492, 537, 769, 1266
Bst2UI CCWGG 1 cut(s) 847
Bst4CI ACNGT 6 cut(s) 18, 665, 1048, 1312, 1387, 1635
Bst6I CTCTTC 3 cut(s) 583, 1034, 1537
BstAFI CTTAAG 1 cut(s) 505
BstAPI GCANNNNNTGC 2 cut(s) 239, 1097
BstC8I GCNNGC 1 cut(s) 1527
BstDEI CTNAG 6 cut(s) 281, 737, 804, 1154, 1665, 1690
BstDSI CCRYGG 1 cut(s) 450
BstEII GGTNACC 1 cut(s) 868
BstF5I GGATG 9 cut(s) 253, 495, 658, 691, 732, 952, 1141, 1226, 1279
BstFNI CGCG 2 cut(s) 452, 528
BstKTI GATC 4 cut(s) 495, 540, 772, 1269
BstMAI GTCTC 1 cut(s) 517
BstMBI GATC 4 cut(s) 492, 537, 769, 1266
BstMWI GCNNNNNNNGC 6 cut(s) 239, 980, 1088, 1097, 1347, 1502
BstNI CCWGG 1 cut(s) 847
BstNSI RCATGY 2 cut(s) 960, 1180
BstPI GGTNACC 1 cut(s) 868
BstSCI CCNGG 1 cut(s) 845
BstSFI CTRYAG 2 cut(s) 661, 1212
BstUI CGCG 2 cut(s) 452, 528
BstV1I GCAGC 6 cut(s) 242, 415, 580, 851, 1100, 1232
Bsu36I CCTNAGG 1 cut(s) 1665
BsuRI GGCC 3 cut(s) 617, 938, 1322
BtgI CCRYGG 1 cut(s) 450
BtsCI GGATG 9 cut(s) 253, 495, 658, 691, 732, 952, 1141, 1226, 1279
BtsIMutI CAGTG 1 cut(s) 1389
BveI ACCTGC 1 cut(s) 1683
Cac8I GCNNGC 1 cut(s) 1527
CaiI CAGNNNCTG 1 cut(s) 428
Cfr13I GGNCC 2 cut(s) 273, 1658
Cfr42I CCGCGG 1 cut(s) 453
CseI GACGC 1 cut(s) 1226
Csp6I GTAC 3 cut(s) 1199, 1292, 1670
CviQI GTAC 3 cut(s) 1199, 1292, 1670
DdeI CTNAG 6 cut(s) 281, 737, 804, 1154, 1665, 1690
DpnI GATC 4 cut(s) 494, 539, 771, 1268
DpnII GATC 4 cut(s) 492, 537, 769, 1266
DraI TTTAAA 2 cut(s) 580, 1600
EaeI YGGCCR 3 cut(s) 615, 936, 1320
Eam1104I CTCTTC 3 cut(s) 583, 1034, 1537
EarI CTCTTC 3 cut(s) 583, 1034, 1537
Ecl136II GAGCTC 1 cut(s) 1151
Eco24I GRGCYC 1 cut(s) 1153
Eco31I GGTCTC 1 cut(s) 517
Eco32I GATATC 1 cut(s) 658
Eco47I GGWCC 2 cut(s) 273, 1658
Eco53kI GAGCTC 1 cut(s) 1151
Eco57I CTGAAG 1 cut(s) 363
Eco81I CCTNAGG 1 cut(s) 1665
Eco91I GGTNACC 1 cut(s) 868
EcoICRI GAGCTC 1 cut(s) 1151
EcoO65I GGTNACC 1 cut(s) 868
EcoRI GAATTC 1 cut(s) 66
EcoRII CCWGG 1 cut(s) 845
EcoRV GATATC 1 cut(s) 658
EcoT22I ATGCAT 1 cut(s) 734
EcoT38I GRGCYC 1 cut(s) 1153
FaqI GGGAC 1 cut(s) 945
FbaI TGATCA 1 cut(s) 1266
FokI GGATG 9 cut(s) 260, 482, 665, 698, 719, 959, 1148, 1213, 1286
FriOI GRGCYC 1 cut(s) 1153
FspBI CTAG 7 cut(s) 62, 125, 564, 675, 891, 1616, 1629
GsaI CCCAGC 1 cut(s) 575
GsuI CTGGAG 4 cut(s) 505, 734, 932, 1144
HaeIII GGCC 3 cut(s) 617, 938, 1322
HapII CCGG 3 cut(s) 683, 977, 1708
HgaI GACGC 1 cut(s) 1226
HincII GTYRAC 2 cut(s) 470, 1621
HindII GTYRAC 2 cut(s) 470, 1621
HindIII AAGCTT 2 cut(s) 151, 707
HinfI GANTC 4 cut(s) 518, 692, 950, 1130
HpaII CCGG 3 cut(s) 683, 977, 1708
HphI GGTGA 5 cut(s) 92, 355, 880, 979, 1381
Hpy166II GTNNAC 8 cut(s) 470, 599, 680, 1201, 1333, 1558, 1621, 1672
Hpy188I TCNGA 6 cut(s) 382, 517, 774, 1135, 1308, 1591
Hpy188III TCNNGA 5 cut(s) 713, 790, 823, 1161, 1172
Hpy8I GTNNAC 8 cut(s) 470, 599, 680, 1201, 1333, 1558, 1621, 1672
Hpy99I CGWCG 1 cut(s) 644
HpyAV CCTTC 9 cut(s) 339, 415, 877, 1132, 1241, 1247, 1433, 1649, 1694
HpyCH4III ACNGT 6 cut(s) 18, 665, 1048, 1312, 1387, 1635
HpyCH4IV ACGT 2 cut(s) 905, 1649
HpyCH4V TGCA 8 cut(s) 220, 242, 336, 460, 609, 732, 960, 1100
HpyF10VI GCNNNNNNNGC 6 cut(s) 239, 980, 1088, 1097, 1347, 1502
HpyF3I CTNAG 6 cut(s) 281, 737, 804, 1154, 1665, 1690
HpySE526I ACGT 2 cut(s) 905, 1649
KpnI GGTACC 1 cut(s) 1295
Ksp22I TGATCA 1 cut(s) 1266
KspI CCGCGG 1 cut(s) 453
Kzo9I GATC 4 cut(s) 492, 537, 769, 1266
LguI GCTCTTC 2 cut(s) 1034, 1537
LmnI GCTCC 5 cut(s) 979, 1031, 1085, 1163, 1355
Lsp1109I GCAGC 6 cut(s) 242, 415, 580, 851, 1100, 1232
LweI GCATC 8 cut(s) 447, 676, 719, 741, 1108, 1177, 1232, 1483
MaeI CTAG 7 cut(s) 62, 125, 564, 675, 891, 1616, 1629
MaeII ACGT 2 cut(s) 905, 1649
MaeIII GTNAC 8 cut(s) 3, 212, 868, 906, 1237, 1312, 1361, 1387
MalI GATC 4 cut(s) 494, 539, 771, 1268
MboI GATC 4 cut(s) 492, 537, 769, 1266
MfeI CAATTG 1 cut(s) 1565
MhlI GDGCHC 2 cut(s) 1046, 1153
MlsI TGGCCA 3 cut(s) 617, 938, 1322
MluCI AATT 8 cut(s) 66, 170, 349, 371, 611, 877, 1410, 1565
MluNI TGGCCA 3 cut(s) 617, 938, 1322
MlyI GAGTC 3 cut(s) 512, 686, 944
MmeI TCCRAC 2 cut(s) 1459, 1632
Mox20I TGGCCA 3 cut(s) 617, 938, 1322
Mph1103I ATGCAT 1 cut(s) 734
MscI TGGCCA 3 cut(s) 617, 938, 1322
MseI TTAA 4 cut(s) 506, 579, 1248, 1599
MslI CAYNNNNRTG 2 cut(s) 833, 965
Msp20I TGGCCA 3 cut(s) 617, 938, 1322
MspA1I CMGCKG 5 cut(s) 428, 452, 571, 944, 1190
MspCI CTTAAG 1 cut(s) 505
MspI CCGG 3 cut(s) 683, 977, 1708
MspR9I CCNGG 1 cut(s) 847
MunI CAATTG 1 cut(s) 1565
Mva1269I GAATGC 1 cut(s) 1102
MvaI CCWGG 1 cut(s) 847
MvnI CGCG 2 cut(s) 452, 528
MwoI GCNNNNNNNGC 6 cut(s) 239, 980, 1088, 1097, 1347, 1502
NdeII GATC 4 cut(s) 492, 537, 769, 1266
NlaIV GGNNCC 3 cut(s) 548, 975, 1293
NmeAIII GCCGAG 1 cut(s) 834
NmuCI GTSAC 3 cut(s) 868, 1237, 1387
NsiI ATGCAT 1 cut(s) 734
NspI RCATGY 2 cut(s) 960, 1180
OliI CACNNNNGTG 1 cut(s) 965
PaqCI CACCTGC 1 cut(s) 1683
PciSI GCTCTTC 2 cut(s) 1034, 1537
PctI GAATGC 1 cut(s) 1102
PfeI GAWTC 1 cut(s) 1130
PleI GAGTC 3 cut(s) 512, 686, 944
PpsI GAGTC 3 cut(s) 512, 686, 944
PsiI TTATAA 1 cut(s) 1511
Psp124BI GAGCTC 1 cut(s) 1153
Psp1406I AACGTT 1 cut(s) 905
Psp6I CCWGG 1 cut(s) 845
PspEI GGTNACC 1 cut(s) 868
PspFI CCCAGC 1 cut(s) 571
PspGI CCWGG 1 cut(s) 845
PspN4I GGNNCC 3 cut(s) 548, 975, 1293
PspPI GGNCC 2 cut(s) 273, 1658
PstNI CAGNNNCTG 1 cut(s) 428
PvuII CAGCTG 2 cut(s) 571, 1190
RsaI GTAC 3 cut(s) 1200, 1293, 1671
RsaNI GTAC 3 cut(s) 1199, 1292, 1670
RseI CAYNNNNRTG 2 cut(s) 833, 965
SacI GAGCTC 1 cut(s) 1153
SacII CCGCGG 1 cut(s) 453
SapI GCTCTTC 2 cut(s) 1034, 1537
SaqAI TTAA 4 cut(s) 506, 579, 1248, 1599
Sau3AI GATC 4 cut(s) 492, 537, 769, 1266
Sau96I GGNCC 2 cut(s) 273, 1658
SchI GAGTC 3 cut(s) 512, 686, 944
ScrFI CCNGG 1 cut(s) 847
SduI GDGCHC 2 cut(s) 1046, 1153
SfaNI GCATC 8 cut(s) 447, 676, 719, 741, 1108, 1177, 1232, 1483
SfcI CTRYAG 2 cut(s) 661, 1212
Sfr303I CCGCGG 1 cut(s) 453
SgrBI CCGCGG 1 cut(s) 453
SinI GGWCC 2 cut(s) 273, 1658
SmiMI CAYNNNNRTG 2 cut(s) 833, 965
SmlI CTYRAG 4 cut(s) 37, 505, 790, 821
SmoI CTYRAG 4 cut(s) 37, 505, 790, 821
SpeI ACTAGT 1 cut(s) 1615
Sse9I AATT 8 cut(s) 66, 170, 349, 371, 611, 877, 1410, 1565
SspMI CTAG 7 cut(s) 62, 125, 564, 675, 891, 1616, 1629
SstI GAGCTC 1 cut(s) 1153
StyD4I CCNGG 1 cut(s) 845
TaaI ACNGT 6 cut(s) 18, 665, 1048, 1312, 1387, 1635
TaiI ACGT 2 cut(s) 908, 1652
TaqI TCGA 2 cut(s) 540, 1552
TasI AATT 8 cut(s) 66, 170, 349, 371, 611, 877, 1410, 1565
TauI GCSGC 6 cut(s) 304, 428, 452, 455, 528, 1341
TfiI GAWTC 1 cut(s) 1130
Tru1I TTAA 4 cut(s) 506, 579, 1248, 1599
Tru9I TTAA 4 cut(s) 506, 579, 1248, 1599
TscAI CASTG 1 cut(s) 1396
TseFI GTSAC 3 cut(s) 868, 1237, 1387
TseI GCWGC 6 cut(s) 230, 428, 568, 839, 1088, 1220
Tsp45I GTSAC 3 cut(s) 868, 1237, 1387
TspDTI ATGAA 6 cut(s) 162, 321, 521, 743, 935, 1434
TspRI CASTG 1 cut(s) 1396
Vha464I CTTAAG 1 cut(s) 505
VpaK11BI GGWCC 2 cut(s) 273, 1658
XapI RAATTY 3 cut(s) 66, 170, 877
XceI RCATGY 2 cut(s) 960, 1180
XspI CTAG 7 cut(s) 62, 125, 564, 675, 891, 1616, 1629
Zsp2I ATGCAT 1 cut(s) 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.