Rmu_sc0001571.1_g000003

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001571.1
OG0016003
Physical Location & Seq
Reverse (-)
6069 .. 22393
16325 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5517 bp
atgtctgagattgtggttgcagagcccatctatgatgatcgagcagaggaaaaaagacccattacaaggtatctcaccatgggcagtgggagtgatcataatcatcctcctgagccatttgagagcgagagattgccttccactttggcctcaaaaatccggagctttcttcgggttgcaaacttgatcgaaagcgaggagcctcggattgcttatctgtgccgctttcatgcctttgaaatagcacataacatggaccgtcgttcgaatgggaggggtgttcgacaattcaaaactacccttctgcagcgacttgaacaggatgaagaaactacaattaggaaacggaaggaaaagagtgatatacgcgagttaagacgcatttatcatgcttacaaggagtacatcatcaaacatgaaggagcatttagcatagaaaatagtcacagagagaagttgataaatgcgcgtataattggttctgtattgtttgaagtcttaaagacagtttcaaatacagccggtcctcaggccctggctaatagaggtggtatccagacaaaaccttatgatatatataatattctaccacttgatcaaggaggtattcagcaaccaatcatgcagctacctgagattaaggcggcagtagcagcaatacggtatattcgtggcataccttccaatgaagatcttcagaaacatgggactttcattgatttgtttgattttcttcagtactgctttggatttcaggaagggaatgtagccaatcagagagagcatctgcttctactccttgctaatattcacaaacggaaaactcataaacagacatctgtctcaaaattaggagatgctgcagtggacgagttaatgcggagaatttttaaaaattatacaaactggtgcaagtttttgggaaggaaaagcaacatccggttaccttatgtgaaacaagaagcccaacaatataaacttctatttctggggctttaccttcttatatggggcgaggctgcaaacttgcgattcatgccagagtgtctctgttatatttttcaccatatggcatatgagctacatggcatgttaactggtgctgttagcttgacaacatgggagaaggtcatgccagcatatggagggcagtctgagtctttccttaacaatgttgttgcccccatatatggggttataagagaggaagccgagaaaagcaagtgtgggacagctgaccattccacatggagaaactatgacgatctgaatgagtatttttggtcttctgattgttttgaaatcggttggcccatgcgtctagatcacgatttcttctttacagactctccaacaaaatccaatgctaagaatgcttcagcatccactgctcctgtagaagaaagaaggaaggaagatgaggaggaagatgaagttggggttactaaagaagaagtccgtgaacccaaatggttgggcaaaacaaatttcgtagaagtacgttcgttttggcaaatttttagaagctttgacagaatgtggagcttttttattgtttcacttcaggcattaataattatggcatgccatgaattggaatctccacttcagttgtttgacaaagttatatttgaggatatcatgagcatcttcattacatctgcatttcttaaattcatacaagcaatactcgacattgcctttacatggaaagtgagacaaacactggacttctctgcaaaagtaaaacatgttatgaagctcggtgttgcaatgatatggactatagttcttcctataaaaccccttatagagccaacaaagcagatcatgaaaattggtgtgccaatgtatgattggcatgagctttttccgaaagttaagagtaatgctggtgcgattgctgctatatgggctccaattatagttgtgtactttatggacactcagatatggtattctgttttttgcacaatctttggtggagtctatggcattttgcatcaccttggtgagattcgaactttgggaatgttgaggagtagatttcactccttgccttccgcattcaatatttctcttattccaccctcattgaggaatgaccaaaagagaagaagaggtttcttttacaataaattcagaaaggtatcaaagagtgaaaaaaatggtcttgccaagtttgtcttggtttggaaccaaattatcaatagttttcgattggaggacttgataaacaacagggagctggatttaatgacaatgccaatgtcttcagaattattttccggcatagttcgttggccagtttttcttcttgcaaataagttttcaactgccctgagtattgcaaaggattttgtggggagggatgaaagtcttgtcagaagacttaaaaaagatgagtacatgtactgtgctgtgaaggagtgctacgaatcattgaagtacatccttgaaatcctcattattggggatctcgaaaagaggattgtatctgccgtactgactgaaattgaagaaagcatcacaagatcaagtcttcttgagaactttaggatgattaaagttccagatcttttggctaaatgtatcgagctaattgaacttttggttgagggaaatgaagaccatcgtggcaaagttgtgaaagttctccaagatatatttgagcttgtaatcgatgatatgatgacgactggcttcaggatattagagttgctggattcatcccaacagactgaaacagatgctgcttatttttctggaagcattgaatccccattattcggatctgctggtggcaagaattctatccatttccctttgccagacagtgctgctctgaatgagcagatcaagcgttttcttctgttgcttactgtccaagatacagcaatggacattccaactaacttagaggctcggagacgcatatcattctttgccacttctctctttatgaatatgccacatgcacccacagtgtccaatatggtgccattcagtgttatgacaccacattacctggaggatattaatttttcaatggaggagctgcattcaagccagcaagaagtttctatcatcttttacatgcaaaagatatttccagatgagtggaaaaacttcctggagcgcatgggttatgaaaatttggatgggttggagaaggacaaaaaggaagagcttagaaattgggcttctttccggggccaaacactgagcagaacagttagaggaatgatgtactatagagaagccttaaaactacaagcatttcttgacatggctgaagatgaagatattcttgaaggttatgatgctgttgaaagtagaaatcatgcattatctgctcagctagatgcattagcagacatgaaattcacatatgttgttacttgccaattatttggatcacaaaaagctgctggagacccacatgcacaggacctaattgacttgatgaaaagatacccaactctccgtgttgcttatgtcgaggagaaagaagagatagtggagaacaagccttgcaaagtttattcttcagtattggttaaagctatccccaagtatggtgatcaggaaatatatcgcataaagcttcctggtgcaccaactattggagaagggaagcccgaaaaccagaaccatggaataatcttcactcgtggtgaagctctccaaaccattgatatgaaccaagacagttatttggaggaagccttcaaaatgagaaatcttctgcaagaatttcttcagaaccgaggacggcgacctcctatactgcttggtttgagggaacacatatttacaggaagtgtttcttctcttgcatggttcatgtcttatcaagagaccagctttgtcaccattggtcaaaggcttcttgccaatcctctcagggtacgattccactatggacatccagatgtatttgacaggttattccacatcacaagaggtggattaagcaaagcatcaaaaacaattaacctgagcgaggatgtttttgctgggtataattctaccttacgacggggatggatcacgtatcatgaatacatgcaagttggcaagggtcgtgatgtaggtctaaaccagatctcaaagtttgaagctaaggttgctaacggtaacagtgaacaaactctaagccgtgacatataccgccttggacgtcagtttgatttcttcaggatgctgtcttgttatttcacaaccattgggttttactttagcagcctgatatcagttattggaatctatgtgttcctttacggacagctatacctagttctcagtggcttggaaaaagccctcataattgaggctagactgcagaacatacaatccttggaaacagctcttgcctcccagtcatttatacagcttgggctattaacaggaatgccaatggtgatggaaattggacttgagaaagggttcctcaatgcactaaaagattttgtcctgatgcaattgcagctggcttctgtttttttcacgttctcatttggaacaaaaatccattattacggccgaacaattctgcatggtggtgctaaatacagacccactgggcgtaaggttgtggtgtttcatactagcttcactgaaaactacaggttgtattcaagaagccactttgtcaaaggatttgagttactgctcctgttgattgtttataacttgtttagacggtcctatgagagcgacatggcgtatgtattagtcacttattcgatttggttcatgtcaatcacttggttgttcgctccatttctttttaatccgtctggattcagttgggacaagatagtagatgactggaaagattggaataagtggatcaggcagcagggtggtattggagttcaccaagataaaagttggcagtcttggtggatagatgagcaggaccatcttcgtcattctgggatgagttctaggttgtttgaaatactcctttctgttcggtttttcctgtatcagtatggtctggtctaccaccttgacatttcccagaatagcaggaattttctggtttatttgctttcatggatagtgatcctcgttgttttcttgttagtaaaggcagtgaacctcggaaggcaaaagttcagtgctagatatcaccttgtgtttaggctattcaaagcaatccttttcttgggtgttctttccatcataataactctttctcatgtgtgtcatctatcttggatggacttggttgtgtgctgcctagcatttttgccaacagggtggggtttgatattgtttgcacaagctgtgaggcccctgatagagaatactggattgtgggaattcaccagagtacttgctaaaacatatgactacggaatgggggttgttctctttacacctattgctgtcttggcatggcttccgattatatcagcattccagactcgattcctgttcaatgaggccttcaataggcacttgcagatccaaccaattcttcaggggaagaagaagaacagatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1838

Amino Acids

212.61

Weight (kDa)

8.42

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 52 - 112 1.8e-07 Vta1 like
FKS1_dom1 PF14288 326 - 437 1.5e-41 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 769 - 962 1.1e-47 Callose synthase 1 domain
Glucan_synthase PF02364 969 - 1656 1.7e-216 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016003)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14570 AT3G14570 AT3G14570
fragaria_vesca FvH4_3g16960
malus_domestica MD11G1276200.v1.1
prunus_persica Prupe.4G152000_v2.0.a1
pyrus_communis pycom11g24450
rosa_chinensis RchiOBHm_Chr5g0028271
rosa_laevigata RLG00000033078
rosa_multiflora Rmu_sc0001571.1_g000003
rosa_roxburghii Rroxscaffold_1G00051450
rosa_rugosa Rorug05G0105400
rosa_samantha Rh5AG198900 Rh5DG199100
rosa_wichuraiana Rw5G018140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1199, 4692
AatII GACGTC 1 cut(s) 4186
AccB1I GGYRCC 1 cut(s) 2998
AccB7I CCANNNNNTGG 3 cut(s) 1142, 1594, 3626
AccI GTMKAC 1 cut(s) 5010
AccII CGCG 2 cut(s) 369, 469
AccIII TCCGGA 1 cut(s) 159
AciI CCGC 5 cut(s) 223, 644, 880, 2064, 4174
AclWI GGATC 7 cut(s) 2484, 2800, 3424, 4058, 4862, 5068, 5473
AcoI YGGCCR 2 cut(s) 2303, 4543
AcyI GRCGYC 1 cut(s) 4183
AdeI CACNNNGTG 1 cut(s) 5146
AflIII ACRYGT 2 cut(s) 1750, 2409
AjnI CCWGG 4 cut(s) 534, 3027, 3132, 3610
AjuI GAANNNNNNNTTGG 4 cut(s) 1419, 1451, 5475, 5507
AleI CACNNNNGTG 1 cut(s) 2010
AloI GAACNNNNNNTCC 2 cut(s) 4340, 4372
Alw21I GWGCWC 1 cut(s) 3619
Alw26I GTCTC 6 cut(s) 847, 1052, 1711, 2923, 3429, 3854
Alw44I GTGCAC 1 cut(s) 3615
AlwI GGATC 7 cut(s) 2484, 2800, 3424, 4058, 4862, 5068, 5473
AlwNI CAGNNNCTG 2 cut(s) 535, 2753
Aor13HI TCCGGA 1 cut(s) 159
AoxI GGCC 8 cut(s) 147, 531, 1310, 2303, 3214, 4543, 5303, 5457
ApaLI GTGCAC 1 cut(s) 3615
AseI ATTAAT 2 cut(s) 1571, 3039
Asp700I GAANNNNTTC 6 cut(s) 693, 3128, 3306, 3759, 3826, 4448
AspLEI GCGC 2 cut(s) 469, 3141
AspS9I GGNCC 9 cut(s) 256, 524, 532, 1311, 3214, 3451, 4707, 4924, 5304
AsuC2I CCSGG 1 cut(s) 3212
AsuII TTCGAA 2 cut(s) 266, 2020
AvaII GGWCC 5 cut(s) 256, 524, 3451, 4707, 4924
AxyI CCTNAGG 1 cut(s) 528
BaeGI GKGCMC 1 cut(s) 3619
BalI TGGCCA 1 cut(s) 2305
BanI GGYRCC 1 cut(s) 2998
BanII GRGCYC 2 cut(s) 27, 1918
BarI GAAGNNNNNNTAC 2 cut(s) 2417, 2449
BauI CACGAG 1 cut(s) 3672
BbsI GAAGAC 5 cut(s) 1278, 2265, 2395, 2534, 2634
Bbv12I GWGCWC 1 cut(s) 3619
BccI CCATC 8 cut(s) 35, 2640, 3155, 4041, 4420, 4935, 5198, 5224
BceAI ACGGC 4 cut(s) 2486, 3791, 4146, 4558
BciT130I CCWGG 4 cut(s) 536, 3029, 3134, 3612
BciVI GTATCC 1 cut(s) 563
BclI TGATCA 3 cut(s) 94, 595, 3583
BcnI CCSGG 1 cut(s) 3212
BcoDI GTCTC 6 cut(s) 847, 1052, 1711, 2923, 3429, 3854
BfaI CTAG 8 cut(s) 1322, 3362, 4298, 4338, 4611, 4953, 5133, 5252
BfmI CTRYAG 7 cut(s) 305, 861, 1395, 1785, 3253, 4343, 4627
BfuI GTATCC 1 cut(s) 563
BglII AGATCT 3 cut(s) 691, 2575, 4107
BlpI GCTNAGC 1 cut(s) 3357
BmcAI AGTACT 2 cut(s) 740, 5346
Bme1390I CCNGG 5 cut(s) 536, 3029, 3134, 3212, 3612
Bme18I GGWCC 5 cut(s) 256, 524, 3451, 4707, 4924
BmgT120I GGNCC 9 cut(s) 256, 524, 532, 1311, 3214, 3451, 4707, 4924, 5304
BmiI GGNNCC 7 cut(s) 201, 1917, 2198, 3000, 3215, 4451, 5306
BmrFI CCNGG 5 cut(s) 536, 3029, 3134, 3212, 3612
BmrI ACTGGG 2 cut(s) 4375, 4593
BmuI ACTGGG 2 cut(s) 4375, 4593
BoxI GACNNNNGTC 1 cut(s) 839
BpiI GAAGAC 5 cut(s) 1278, 2265, 2395, 2534, 2634
BpmI CTGGAG 3 cut(s) 3050, 3155, 3453
Bpu10I CCTNAGC 3 cut(s) 111, 4001, 4125
Bpu1102I GCTNAGC 1 cut(s) 3357
Bpu14I TTCGAA 2 cut(s) 266, 2020
BpuEI CTTGAG 2 cut(s) 2567, 4460
BpuMI CCSGG 1 cut(s) 3212
Bsa29I ATCGAT 1 cut(s) 2682
BsaAI YACGTR 1 cut(s) 4056
BsaBI GATNNNNATC 2 cut(s) 99, 5072
BsaHI GRCGYC 1 cut(s) 4183
BsaI GGTCTC 2 cut(s) 3429, 3854
BsaWI WCCGGW 2 cut(s) 159, 939
BsaXI ACNNNNNCTCC 2 cut(s) 1333, 1363
Bse118I RCCGGY 1 cut(s) 521
Bse1I ACTGG 9 cut(s) 911, 1102, 1731, 2306, 2704, 4381, 4588, 4838, 5326
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 3 cut(s) 1695, 1779, 2904
Bse8I GATNNNNATC 2 cut(s) 99, 5072
BseAI TCCGGA 1 cut(s) 159
BseBI CCWGG 4 cut(s) 536, 3029, 3134, 3612
BseCI ATCGAT 1 cut(s) 2682
BseJI GATNNNNATC 2 cut(s) 99, 5072
BseMI GCAATG 3 cut(s) 1695, 1779, 2904
BseNI ACTGG 9 cut(s) 911, 1102, 1731, 2306, 2704, 4381, 4588, 4838, 5326
BseRI GAGGAG 5 cut(s) 212, 1436, 2053, 3068, 3518
BseSI GKGCMC 1 cut(s) 3619
BseX3I CGGCCG 1 cut(s) 4543
BseYI CCCAGC 1 cut(s) 4019
Bsh1236I CGCG 2 cut(s) 369, 469
Bsh1285I CGRYCG 1 cut(s) 4546
BshFI GGCC 8 cut(s) 149, 533, 1312, 2305, 3216, 4545, 5305, 5459
BshNI GGYRCC 1 cut(s) 2998
BshVI ATCGAT 1 cut(s) 2682
BsiEI CGRYCG 1 cut(s) 4546
BsiHKAI GWGCWC 1 cut(s) 3619
BsiSI CCGG 5 cut(s) 160, 522, 940, 2289, 3211
BslFI GGGAC 3 cut(s) 721, 1243, 4829
BsmAI GTCTC 6 cut(s) 847, 1052, 1711, 2923, 3429, 3854
BsmBI CGTCTC 1 cut(s) 2923
BsmFI GGGAC 3 cut(s) 721, 1243, 4829
BsmI GAATGC 5 cut(s) 1378, 2066, 3061, 4419, 5429
BsnI GGCC 8 cut(s) 149, 533, 1312, 2305, 3216, 4545, 5305, 5459
Bso31I GGTCTC 2 cut(s) 3429, 3854
Bsp119I TTCGAA 2 cut(s) 266, 2020
Bsp1286I GDGCHC 3 cut(s) 27, 1918, 3619
Bsp13I TCCGGA 1 cut(s) 159
Bsp1720I GCTNAGC 1 cut(s) 3357
Bsp19I CCATGG 2 cut(s) 78, 3655
BspACI CCGC 5 cut(s) 223, 644, 880, 2064, 4174
BspANI GGCC 8 cut(s) 149, 533, 1312, 2305, 3216, 4545, 5305, 5459
BspDI ATCGAT 1 cut(s) 2682
BspEI TCCGGA 1 cut(s) 159
BspFNI CGCG 2 cut(s) 369, 469
BspHI TCATGA 3 cut(s) 1641, 1830, 4060
BspLI GGNNCC 7 cut(s) 201, 1917, 2198, 3000, 3215, 4451, 5306
BspMAI CTGCAG 3 cut(s) 309, 865, 4347
BspPI GGATC 7 cut(s) 2484, 2800, 3424, 4058, 4862, 5068, 5473
BspQI GCTCTTC 1 cut(s) 3180
BspT104I TTCGAA 2 cut(s) 266, 2020
BspT107I GGYRCC 1 cut(s) 2998
BspTNI GGTCTC 2 cut(s) 3429, 3854
BsrDI GCAATG 3 cut(s) 1695, 1779, 2904
BsrFI RCCGGY 1 cut(s) 521
BsrI ACTGG 9 cut(s) 911, 1102, 1731, 2306, 2704, 4381, 4588, 4838, 5326
BssAI RCCGGY 1 cut(s) 521
BssNI GRCGYC 1 cut(s) 4183
BssSI CACGAG 1 cut(s) 3672
BssT1I CCWWGG 5 cut(s) 78, 2008, 3655, 4177, 4359
Bst2BI CACGAG 1 cut(s) 3672
Bst2UI CCWGG 4 cut(s) 536, 3029, 3134, 3612
Bst6I CTCTTC 3 cut(s) 2113, 3180, 3507
BstACI GRCGYC 1 cut(s) 4183
BstAPI GCANNNNNTGC 2 cut(s) 1388, 3353
BstBAI YACGTR 1 cut(s) 4056
BstBI TTCGAA 2 cut(s) 266, 2020
BstC8I GCNNGC 4 cut(s) 1137, 1585, 3071, 4494
BstDSI CCRYGG 2 cut(s) 78, 3655
BstEII GGTNACC 1 cut(s) 942
BstENI CCTNNNNNAGG 3 cut(s) 2095, 4004, 5464
BstFNI CGCG 2 cut(s) 369, 469
BstHHI GCGC 2 cut(s) 469, 3141
BstMAI GTCTC 6 cut(s) 847, 1052, 1711, 2923, 3429, 3854
BstMCI CGRYCG 1 cut(s) 4546
BstNI CCWGG 4 cut(s) 536, 3029, 3134, 3612
BstNSI RCATGY 8 cut(s) 1093, 1587, 1754, 2413, 2978, 3100, 3446, 4072
BstPAI GACNNNNGTC 1 cut(s) 839
BstPI GGTNACC 1 cut(s) 942
BstSCI CCNGG 5 cut(s) 534, 3027, 3132, 3210, 3610
BstSFI CTRYAG 7 cut(s) 305, 861, 1395, 1785, 3253, 4343, 4627
BstSLI GKGCMC 1 cut(s) 3619
BstUI CGCG 2 cut(s) 369, 469
BstV2I GAAGAC 5 cut(s) 1278, 2265, 2395, 2534, 2634
BstX2I RGATCY 6 cut(s) 691, 2476, 2575, 2792, 4107, 5478
BstXI CCANNNNNNTGG 5 cut(s) 1474, 3120, 3413, 3656, 5271
BstYI RGATCY 6 cut(s) 691, 2476, 2575, 2792, 4107, 5478
BstZI CGGCCG 1 cut(s) 4543
Bsu15I ATCGAT 1 cut(s) 2682
Bsu36I CCTNAGG 1 cut(s) 528
BsuI GTATCC 1 cut(s) 563
BsuRI GGCC 8 cut(s) 149, 533, 1312, 2305, 3216, 4545, 5305, 5459
BsuTUI ATCGAT 1 cut(s) 2682
BtgI CCRYGG 2 cut(s) 78, 3655
BtsI GCAGTG 4 cut(s) 91, 870, 1386, 5109
Cac8I GCNNGC 4 cut(s) 1137, 1585, 3071, 4494
CaiI CAGNNNCTG 2 cut(s) 535, 2753
CciI TCATGA 3 cut(s) 1641, 1830, 4060
CfoI GCGC 2 cut(s) 469, 3141
Cfr10I RCCGGY 1 cut(s) 521
Cfr13I GGNCC 9 cut(s) 256, 524, 532, 1311, 3214, 3451, 4707, 4924, 5304
ClaI ATCGAT 1 cut(s) 2682
CseI GACGC 3 cut(s) 387, 1307, 2940
CspCI CAANNNNNGTGG 4 cut(s) 1593, 1628, 2076, 2111
DraI TTTAAA 1 cut(s) 892
DraIII CACNNNGTG 1 cut(s) 5146
EaeI YGGCCR 2 cut(s) 2303, 4543
EagI CGGCCG 1 cut(s) 4543
Eam1104I CTCTTC 3 cut(s) 2113, 3180, 3507
EarI CTCTTC 3 cut(s) 2113, 3180, 3507
EclXI CGGCCG 1 cut(s) 4543
Eco130I CCWWGG 5 cut(s) 78, 2008, 3655, 4177, 4359
Eco147I AGGCCT 1 cut(s) 5459
Eco24I GRGCYC 2 cut(s) 27, 1918
Eco31I GGTCTC 2 cut(s) 3429, 3854
Eco32I GATATC 3 cut(s) 1639, 4254, 5138
Eco47I GGWCC 5 cut(s) 256, 524, 3451, 4707, 4924
Eco52I CGGCCG 1 cut(s) 4543
Eco81I CCTNAGG 1 cut(s) 528
Eco91I GGTNACC 1 cut(s) 942
EcoNI CCTNNNNNAGG 3 cut(s) 2095, 4004, 5464
EcoO109I RGGNCCY 3 cut(s) 532, 3451, 5304
EcoO65I GGTNACC 1 cut(s) 942
EcoRI GAATTC 2 cut(s) 2809, 5333
EcoRII CCWGG 4 cut(s) 534, 3027, 3132, 3610
EcoRV GATATC 3 cut(s) 1639, 4254, 5138
EcoT14I CCWWGG 5 cut(s) 78, 2008, 3655, 4177, 4359
EcoT22I ATGCAT 2 cut(s) 3349, 3370
EcoT38I GRGCYC 2 cut(s) 27, 1918
ErhI CCWWGG 5 cut(s) 78, 2008, 3655, 4177, 4359
Esp3I CGTCTC 1 cut(s) 2923
FaqI GGGAC 3 cut(s) 721, 1243, 4829
FauNDI CATATG 5 cut(s) 1068, 1075, 1141, 3391, 5359
FbaI TGATCA 3 cut(s) 94, 595, 3583
FblI GTMKAC 1 cut(s) 5010
FriOI GRGCYC 2 cut(s) 27, 1918
FspBI CTAG 8 cut(s) 1322, 3362, 4298, 4338, 4611, 4953, 5133, 5252
GlaI GCGC 2 cut(s) 468, 3140
GsaI CCCAGC 1 cut(s) 4023
GsuI CTGGAG 3 cut(s) 3050, 3155, 3453
HaeIII GGCC 8 cut(s) 149, 533, 1312, 2305, 3216, 4545, 5305, 5459
HapII CCGG 5 cut(s) 160, 522, 940, 2289, 3211
HgaI GACGC 3 cut(s) 387, 1307, 2940
HhaI GCGC 2 cut(s) 469, 3141
Hin1I GRCGYC 1 cut(s) 4183
Hin6I GCGC 2 cut(s) 467, 3139
HinP1I GCGC 2 cut(s) 467, 3139
HincII GTYRAC 1 cut(s) 1095
HindII GTYRAC 1 cut(s) 1095
HindIII AAGCTT 2 cut(s) 1525, 3605
HpaI GTTAAC 1 cut(s) 1095
HpaII CCGG 5 cut(s) 160, 522, 940, 2289, 3211
Hpy166II GTNNAC 9 cut(s) 868, 1095, 1463, 1933, 3617, 4148, 4882, 5011, 5107
Hpy8I GTNNAC 9 cut(s) 868, 1095, 1463, 1933, 3617, 4148, 4882, 5011, 5107
Hpy99I CGWCG 2 cut(s) 264, 4044
HpyCH4IV ACGT 4 cut(s) 1501, 4055, 4183, 4511
HpySE526I ACGT 4 cut(s) 1501, 4055, 4183, 4511
Hsp92I GRCGYC 1 cut(s) 4183
HspAI GCGC 2 cut(s) 467, 3139
Kpn2I TCCGGA 1 cut(s) 159
Ksp22I TGATCA 3 cut(s) 94, 595, 3583
KspAI GTTAAC 1 cut(s) 1095
LguI GCTCTTC 1 cut(s) 3180
MaeI CTAG 8 cut(s) 1322, 3362, 4298, 4338, 4611, 4953, 5133, 5252
MaeII ACGT 4 cut(s) 1501, 4055, 4183, 4511
MaeIII GTNAC 9 cut(s) 443, 942, 1441, 3397, 3871, 4139, 4163, 4668, 4738
MfeI CAATTG 1 cut(s) 4484
MflI RGATCY 6 cut(s) 691, 2476, 2575, 2792, 4107, 5478
MhlI GDGCHC 3 cut(s) 27, 1918, 3619
MlsI TGGCCA 1 cut(s) 2305
MluNI TGGCCA 1 cut(s) 2305
MlyI GAGTC 4 cut(s) 1166, 1340, 1995, 5431
MmeI TCCRAC 4 cut(s) 1376, 2933, 3147, 5506
Mox20I TGGCCA 1 cut(s) 2305
Mph1103I ATGCAT 2 cut(s) 3349, 3370
MroI TCCGGA 1 cut(s) 159
MroXI GAANNNNTTC 6 cut(s) 693, 3128, 3306, 3759, 3826, 4448
MscI TGGCCA 1 cut(s) 2305
MslI CAYNNNNRTG 4 cut(s) 2010, 3698, 3933, 4563
Msp20I TGGCCA 1 cut(s) 2305
MspA1I CMGCKG 2 cut(s) 1235, 4492
MspI CCGG 5 cut(s) 160, 522, 940, 2289, 3211
MspR9I CCNGG 5 cut(s) 536, 3029, 3134, 3212, 3612
MunI CAATTG 1 cut(s) 4484
Mva1269I GAATGC 5 cut(s) 1378, 2066, 3061, 4419, 5429
MvaI CCWGG 4 cut(s) 536, 3029, 3134, 3612
MvnI CGCG 2 cut(s) 369, 469
NciI CCSGG 1 cut(s) 3212
NcoI CCATGG 2 cut(s) 78, 3655
NdeI CATATG 5 cut(s) 1068, 1075, 1141, 3391, 5359
NlaIV GGNNCC 7 cut(s) 201, 1917, 2198, 3000, 3215, 4451, 5306
NmeAIII GCCGAG 1 cut(s) 1237
NmuCI GTSAC 4 cut(s) 443, 3871, 4163, 4738
NsiI ATGCAT 2 cut(s) 3349, 3370
NspI RCATGY 8 cut(s) 1093, 1587, 1754, 2413, 2978, 3100, 3446, 4072
NspV TTCGAA 2 cut(s) 266, 2020
OliI CACNNNNGTG 1 cut(s) 2010
PaeI GCATGC 1 cut(s) 1587
PagI TCATGA 3 cut(s) 1641, 1830, 4060
PceI AGGCCT 1 cut(s) 5459
PciI ACATGT 2 cut(s) 1750, 2409
PciSI GCTCTTC 1 cut(s) 3180
PcsI WCGNNNNNNNCGW 1 cut(s) 667
PctI GAATGC 5 cut(s) 1378, 2066, 3061, 4419, 5429
PdmI GAANNNNTTC 6 cut(s) 693, 3128, 3306, 3759, 3826, 4448
PflMI CCANNNNNTGG 3 cut(s) 1142, 1594, 3626
PfoI TCCNGGA 1 cut(s) 3132
PleI GAGTC 4 cut(s) 1165, 1340, 1994, 5431
PpsI GAGTC 4 cut(s) 1165, 1340, 1994, 5431
Ppu21I YACGTR 1 cut(s) 4056
PpuMI RGGWCCY 1 cut(s) 3451
PscI ACATGT 2 cut(s) 1750, 2409
PshAI GACNNNNGTC 1 cut(s) 839
PshBI ATTAAT 2 cut(s) 1571, 3039
PsiI TTATAA 2 cut(s) 1199, 4692
Psp5II RGGWCCY 1 cut(s) 3451
Psp6I CCWGG 4 cut(s) 534, 3027, 3132, 3610
PspEI GGTNACC 1 cut(s) 942
PspFI CCCAGC 1 cut(s) 4019
PspGI CCWGG 4 cut(s) 534, 3027, 3132, 3610
PspN4I GGNNCC 7 cut(s) 201, 1917, 2198, 3000, 3215, 4451, 5306
PspPI GGNCC 9 cut(s) 256, 524, 532, 1311, 3214, 3451, 4707, 4924, 5304
PspPPI RGGWCCY 1 cut(s) 3451
PsrI GAACNNNNNNTAC 2 cut(s) 463, 495
PstI CTGCAG 3 cut(s) 309, 865, 4347
PstNI CAGNNNCTG 2 cut(s) 535, 2753
PsuI RGATCY 6 cut(s) 691, 2476, 2575, 2792, 4107, 5478
PvuII CAGCTG 2 cut(s) 1235, 4492
RseI CAYNNNNRTG 4 cut(s) 2010, 3698, 3933, 4563
SapI GCTCTTC 1 cut(s) 3180
Sau96I GGNCC 9 cut(s) 256, 524, 532, 1311, 3214, 3451, 4707, 4924, 5304
ScaI AGTACT 2 cut(s) 740, 5346
SchI GAGTC 4 cut(s) 1166, 1340, 1995, 5431
ScrFI CCNGG 5 cut(s) 536, 3029, 3134, 3212, 3612
SduI GDGCHC 3 cut(s) 27, 1918, 3619
SfcI CTRYAG 7 cut(s) 305, 861, 1395, 1785, 3253, 4343, 4627
SfuI TTCGAA 2 cut(s) 266, 2020
SinI GGWCC 5 cut(s) 256, 524, 3451, 4707, 4924
SmiMI CAYNNNNRTG 4 cut(s) 2010, 3698, 3933, 4563
SmlI CTYRAG 2 cut(s) 2546, 4439
SmoI CTYRAG 2 cut(s) 2546, 4439
SphI GCATGC 1 cut(s) 1587
SseBI AGGCCT 1 cut(s) 5459
SsiI CCGC 5 cut(s) 223, 644, 880, 2064, 4174
SspI AATATT 3 cut(s) 583, 808, 2074
SspMI CTAG 8 cut(s) 1322, 3362, 4298, 4338, 4611, 4953, 5133, 5252
StuI AGGCCT 1 cut(s) 5459
StyD4I CCNGG 5 cut(s) 534, 3027, 3132, 3210, 3610
StyI CCWWGG 5 cut(s) 78, 2008, 3655, 4177, 4359
TaiI ACGT 4 cut(s) 1504, 4058, 4186, 4514
TatI WGTACW 8 cut(s) 402, 738, 1932, 2406, 2412, 2448, 3249, 5344
TauI GCSGC 2 cut(s) 225, 647
TseFI GTSAC 4 cut(s) 443, 3871, 4163, 4738
Tsp45I GTSAC 4 cut(s) 443, 3871, 4163, 4738
TspGWI ACGGA 7 cut(s) 361, 832, 1448, 3476, 4299, 4788, 5382
Van91I CCANNNNNTGG 3 cut(s) 1142, 1594, 3626
VneI GTGCAC 1 cut(s) 3615
VpaK11BI GGWCC 5 cut(s) 256, 524, 3451, 4707, 4924
VspI ATTAAT 2 cut(s) 1571, 3039
XagI CCTNNNNNAGG 3 cut(s) 2095, 4004, 5464
XbaI TCTAGA 1 cut(s) 1321
XceI RCATGY 8 cut(s) 1093, 1587, 1754, 2413, 2978, 3100, 3446, 4072
XcmI CCANNNNNNNNNTGG 2 cut(s) 1854, 2185
XmiI GTMKAC 1 cut(s) 5010
XmnI GAANNNNTTC 6 cut(s) 693, 3128, 3306, 3759, 3826, 4448
XspI CTAG 8 cut(s) 1322, 3362, 4298, 4338, 4611, 4953, 5133, 5252
ZraI GACGTC 1 cut(s) 4184
ZrmI AGTACT 2 cut(s) 740, 5346
Zsp2I ATGCAT 2 cut(s) 3349, 3370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.