Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0001637.1

Orthogroup ID

OG0005331

Physical Location & Seq

Genomic Coordinates Forward (+)

2483 .. 10553

Gene Structure 8071 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2664 bp

atggcgtcgctctcgtcgtcggcgatttccaatcacagcctctcccccaatgcctttgtgcctaagaagatctgcttcttctctaatcaatgccgcgcattacttctccctcagcgctctcctatttgcaggatgggctttgtctctgggctggaaaggaaaaagacttctaaaaccaacatctttgcatcagtcacagtagacacttcagtaggaacgccccctgagaaagtccagcttccaaaaggtgacagttggtctgttcataaatttgggggcacttgcgtgggaagttcggaaagaattaataatgttgcaaagataattcttagtgatgagtcggagaggaagtttgtagtggtctctgcaatgtctaaggtaacagatatgatgtacgatctcatcttaaaggctcaatcacgagacaaaacctatttggctgcattagatgctgttttagtaaaacacagatcaacagctgtcgatttacttgatgggaatgaacttggtaacttcttatcagcattaaatcaggacatcagtaacctgaaagctatgcttcgtgcaatatatatagctggtcatgcaacagaatctttcacagattttgtggtaggacatggagagttatggtctgctcagttactggcatctgttgttaggaagaatggagtggattgcaagtggatggatacaagggaagtccttattgtcaaccctactggctctgatcaagtcgatcctgatttcgagaaatctgaaaagagacttgagaaatggtattcaagaaatccatcaaaaacaatcatagctactggttttattgctagtacaccccaagacattcctactactttgaagagagatggaagtgacttctctgcagctatcatgggtgctctattcaaggctggtcaagttacaatttggacagatgttgatggtgtctatagtgcggaccccaggaaagttagtgaagcagtgattctgaagacattgtcttatcaagaggcctgggaaatgtcttattttggggcaaatgttctgcatccacgtaccatcattccagtaatgcgatatgacatcccaattatcataaggaatgttttcaacctctctgcacctggaacaaaaatttgccgctcttctactactgaggatgaagtggaccagagtttggaatcctacgtcagagggtttgcgacaatagataacttggcccttgtaaatgttgaaggaactggaatggctggtgttcctggtacagctagtgctatattttctgccgttaaagatgtgggggctaatgttattatgatatctcaggctagtagcgaacattctgtatgctttgctgtcccggagaaggaagtaaaagctgtttctaaggcactgcaatctaggttccgtgaagctttgaatgctgggcgtctttctcaggttcaagtcattccaaactgtagtatattggcagcggttggccagaaaatggcaagtactcctggagttagtgcaactcttttcaacgccctggcaaaggcaaatattaacgtgcgtgctatagctcaaggttgttctgagtacaacattacagtagtgttaaagcgggaggattgtattagggcccttagagcggttcactctagattttatctttcaagaaccactatagcaatgggcattgttggaccaggacttattggtgctacattacttgatcaactgagagatcaggcagcaactcttaaggaagagtttaacattgacttgcgtgtaatgggaattactggctcaaggacaatgcttttgagtgagacgggtgttgacttatctagatggagagaacttcaacaagaaaagggagaacgggcagatatggaaaaatttgtgcagcatgtacatgggaatcattttattccaaatactgttctagttgactgcacagctgactctgtcattgcaagccattactatggctggttgcggaaaggaatacatgtagtcacccccaacaaaaaggcgaattcagggcctcttgatcaggtaataatcctttggctcctagaaacaggggacaaaatattgcgtattgagggcatcttcagtgggactttgagttatatcttcaacaacttagtaggaagacgaacctttagtgaggtggtggctgaggcaaagcaggcagggtttacggagccagacccaagggatgatctatctggaacagatgtttgtaggaaggtaataattcttgcaagggaatctggtcttaagctggaactctctgatatccctgttgaaagcctcgtgccagaaccgttgaaggatagtgcatcagctgaggaattcatgcagaaacttccacaattcgaccatgacttggctaagaaaagacagatcgccgaggatgcaggggaagtcttgagatacgttggggtggttgatgtagtcaaccagaagggggtagtgaagctgcaagcatacaaaaacgatcacccatttgctcaattatcaggggcagataatatcattgctttcacaacaactaggtacaaagatcagccgctaatagtccgcggaccaggagctggtgctgaagttacagctggtggagtcttcagtgacatactgaggcttgcttcatatcttggtgccccatcatag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004072 GO:0004412 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009069 GO:0009070 GO:0009090 GO:0009092 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016301 GO:0016310 GO:0016491 GO:0016614 GO:0016616 GO:0016740 GO:0016772 GO:0016774 GO:0019202 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0055114 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00260 ko00261 ko00270 ko00300 ko01100 ko01110 ko01120 ko01130 ko01230 map00260 map00261 map00270 map00300 map01100 map01110 map01120 map01130 map01230

Pfam Domains

Protein Families

AA_kinase ACT ACT_7 Homoserine_dh NAD_binding_3

Protein Analysis

887

Amino Acids

96.72

Weight (kDa)

7.55

Isoelectric Point (pI)

32.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_kinase	PF00696	87 - 368	2.3e-43	Amino acid kinase family
ACT_7	PF13840	404 - 466	5.4e-06	ACT domain
ACT_9	PF22468	409 - 469	4.5e-18	ACT domain
ACT_7	PF13840	484 - 548	2.3e-09	ACT domain
ACT_9	PF22468	491 - 550	1.4e-21	ACT domain
ACT	PF01842	497 - 537	3.9e-06	ACT domain
NAD_binding_3	PF03447	563 - 676	6e-17	Homoserine dehydrogenase, NAD binding domain
Homoserine_dh	PF00742	688 - 877	7.5e-50	Homoserine dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005331)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31230 AT4G19710 AT4G19710
fragaria_vesca	FvH4_2g00800 FvH4_2g00800 FvH4_2g00800 FvH4_2g00800
malus_domestica	MD05G1003800.v1.1 MD10G1004700.v1.1
prunus_persica	Prupe.5G007500_v2.0.a1 Prupe.5G007500_v2.0.a1 Prupe.5G007500_v2.0.a1 Prupe.8G007000_v2.0.a1 Prupe.8G007000_v2.0.a1
pyrus_communis	pycom05g00330 pycom10g00370 pycom10g00380
rosa_chinensis	RchiOBHm_Chr6g0251231
rosa_laevigata	RLG00000015472
rosa_multiflora	Rmu_sc0001637.1_g000003 Rmu_sc0015396.1_g000003
rosa_roxburghii	Rroxscaffold_7G00216900
rosa_rugosa	Rorug05G0500900.1 Rorug05G0501000
rosa_samantha	Rh6BG001700 Rh6CG007400 Rh6DG007800
rosa_wichuraiana	Rw6G001010 Rw6G001120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2651
AccB7I	CCANNNNNTGG	4 cut(s)	1177, 1489, 2380, 2588
AccBSI	CCGCTC	2 cut(s)	1143, 1634
AccI	GTMKAC	1 cut(s)	201
AccII	CGCG	2 cut(s)	96, 2577
AclWI	GGATC	1 cut(s)	734
AcoI	YGGCCR	1 cut(s)	1480
AcsI	RAATTY	5 cut(s)	269, 1134, 1883, 2023, 2345
AcuI	CTGAAG	5 cut(s)	192, 1010, 2086, 2602, 2616
AcyI	GRCGYC	2 cut(s)	5, 1429
AfaI	GTAC	8 cut(s)	395, 832, 1057, 1264, 1498, 1583, 1899, 2552
AfeI	AGCGCT	1 cut(s)	116
AfiI	CCNNNNNNNGG	8 cut(s)	1177, 1366, 1489, 1537, 1633, 2380, 2461, 2588
AflII	CTTAAG	2 cut(s)	1745, 2270
AflIII	ACRYGT	1 cut(s)	1996
AhdI	GACNNNNNGTC	1 cut(s)	256
AjnI	CCWGG	8 cut(s)	962, 1013, 1123, 1258, 1501, 1530, 1690, 2581
AleI	CACNNNNGTG	1 cut(s)	284
Alw21I	GWGCWC	1 cut(s)	901
Alw26I	GTCTC	5 cut(s)	148, 367, 417, 760, 1808
AlwI	GGATC	1 cut(s)	734
AlwNI	CAGNNNCTG	2 cut(s)	646, 2588
Aor51HI	AGCGCT	1 cut(s)	116
AoxI	GGCC	5 cut(s)	1011, 1218, 1480, 1623, 2030
ApaI	GGGCCC	1 cut(s)	1627
ApeKI	GCWGC	6 cut(s)	440, 884, 1472, 1736, 1891, 2473
ApoI	RAATTY	5 cut(s)	269, 1134, 1883, 2023, 2345
ArsI	GACNNNNNNTTYG	2 cut(s)	1789, 1821
AseI	ATTAAT	1 cut(s)	306
Asp700I	GAANNNNTTC	1 cut(s)	1106
AspLEI	GCGC	2 cut(s)	98, 117
AspS9I	GGNCC	8 cut(s)	958, 1168, 1219, 1623, 1624, 1688, 2030, 2579
AsuC2I	CCSGG	1 cut(s)	1361
AsuHPI	GGTGA	3 cut(s)	260, 1996, 2486
AvaII	GGWCC	4 cut(s)	958, 1168, 1688, 2579
BaeGI	GKGCMC	3 cut(s)	281, 1627, 2656
BalI	TGGCCA	1 cut(s)	1482
BanI	GGYRCC	1 cut(s)	2651
BanII	GRGCYC	1 cut(s)	1627
BauI	CACGAG	2 cut(s)	420, 2306
BbsI	GAAGAC	3 cut(s)	998, 2149, 2608
Bbv12I	GWGCWC	1 cut(s)	901
BbvCI	CCTCAGC	3 cut(s)	111, 2169, 2340
BbvI	GCAGC	6 cut(s)	427, 896, 1484, 1748, 1903, 2460
BccI	CCATC	8 cut(s)	127, 488, 682, 802, 860, 935, 1067, 1830
BceAI	ACGGC	1 cut(s)	1271
BciT130I	CCWGG	8 cut(s)	964, 1015, 1125, 1260, 1503, 1532, 1692, 2583
BciVI	GTATCC	1 cut(s)	685
BclI	TGATCA	3 cut(s)	730, 1717, 2038
BcnI	CCSGG	1 cut(s)	1361
BcoDI	GTCTC	5 cut(s)	148, 367, 417, 760, 1808
BfaI	CTAG	9 cut(s)	828, 1269, 1329, 1401, 1644, 1833, 1931, 2063, 2547
BfmI	CTRYAG	5 cut(s)	882, 949, 1459, 1560, 1668
BfoI	RGCGCY	1 cut(s)	118
BfrI	CTTAAG	2 cut(s)	1745, 2270
BfuI	GTATCC	1 cut(s)	685
BglII	AGATCT	1 cut(s)	69
BisI	GCNGC	9 cut(s)	94, 441, 885, 1141, 1473, 1737, 1892, 2474, 2564
BlsI	GCNGC	9 cut(s)	95, 442, 886, 1142, 1474, 1738, 1893, 2475, 2565
BmcAI	AGTACT	1 cut(s)	1498
Bme1390I	CCNGG	9 cut(s)	964, 1015, 1125, 1260, 1361, 1503, 1532, 1692, 2583
Bme18I	GGWCC	4 cut(s)	958, 1168, 1688, 2579
BmeRI	GACNNNNNGTC	1 cut(s)	256
BmgT120I	GGNCC	8 cut(s)	958, 1168, 1219, 1623, 1624, 1688, 2030, 2579
BmiI	GGNNCC	6 cut(s)	960, 1406, 1625, 2060, 2196, 2653
BmrFI	CCNGG	9 cut(s)	964, 1015, 1125, 1260, 1361, 1503, 1532, 1692, 2583
BmsI	GCATC	7 cut(s)	197, 439, 659, 1057, 2106, 2342, 2398
BpiI	GAAGAC	3 cut(s)	998, 2149, 2608
BpmI	CTGGAG	1 cut(s)	1524
Bpu10I	CCTNAGC	3 cut(s)	111, 2169, 2340
BpuEI	CTTGAG	4 cut(s)	791, 1551, 1777, 2443
BpuMI	CCSGG	1 cut(s)	1361
BsaAI	YACGTR	1 cut(s)	1055
BsaHI	GRCGYC	2 cut(s)	5, 1429
BsaI	GGTCTC	1 cut(s)	367
BsaJI	CCNNGG	6 cut(s)	962, 1014, 1530, 2204, 2403, 2575
BsaXI	ACNNNNNCTCC	4 cut(s)	26, 56, 335, 365
Bsc4I	CCNNNNNNNGG	8 cut(s)	1177, 1366, 1489, 1537, 1633, 2380, 2461, 2588
Bse1I	ACTGG	6 cut(s)	651, 727, 820, 1067, 1246, 1792
Bse3DI	GCAATG	4 cut(s)	375, 1680, 1956, 2529
BseBI	CCWGG	8 cut(s)	964, 1015, 1125, 1260, 1503, 1532, 1692, 2583
BseDI	CCNNGG	6 cut(s)	962, 1014, 1530, 2204, 2403, 2575
BseGI	GGATG	7 cut(s)	138, 693, 1048, 1083, 1165, 2215, 2413
BseLI	CCNNNNNNNGG	8 cut(s)	1177, 1366, 1489, 1537, 1633, 2380, 2461, 2588
BseMI	GCAATG	4 cut(s)	375, 1680, 1956, 2529
BseNI	ACTGG	6 cut(s)	651, 727, 820, 1067, 1246, 1792
BseSI	GKGCMC	3 cut(s)	281, 1627, 2656
BseXI	GCAGC	6 cut(s)	427, 896, 1484, 1748, 1903, 2460
BseYI	CCCAGC	1 cut(s)	1424
BsgI	GTGCAG	3 cut(s)	1104, 1910, 1924
Bsh1236I	CGCG	2 cut(s)	96, 2577
BshFI	GGCC	5 cut(s)	1013, 1220, 1482, 1625, 2032
BshNI	GGYRCC	1 cut(s)	2651
BsiHKAI	GWGCWC	1 cut(s)	901
BsiSI	CCGG	1 cut(s)	1361
BslFI	GGGAC	3 cut(s)	1343, 2087, 2122
BslI	CCNNNNNNNGG	8 cut(s)	1177, 1366, 1489, 1537, 1633, 2380, 2461, 2588
BsmAI	GTCTC	5 cut(s)	148, 367, 417, 760, 1808
BsmBI	CGTCTC	1 cut(s)	1808
BsmFI	GGGAC	3 cut(s)	1343, 2087, 2122
BsmI	GAATGC	1 cut(s)	1426
BsnI	GGCC	5 cut(s)	1013, 1220, 1482, 1625, 2032
Bso31I	GGTCTC	1 cut(s)	367
Bsp120I	GGGCCC	1 cut(s)	1623
Bsp1286I	GDGCHC	4 cut(s)	281, 901, 1627, 2656
Bsp1407I	TGTACA	1 cut(s)	1897
BspANI	GGCC	5 cut(s)	1013, 1220, 1482, 1625, 2032
BspFNI	CGCG	2 cut(s)	96, 2577
BspLI	GGNNCC	6 cut(s)	960, 1406, 1625, 2060, 2196, 2653
BspMAI	CTGCAG	1 cut(s)	886
BspPI	GGATC	1 cut(s)	734
BspQI	GCTCTTC	1 cut(s)	1150
BspT107I	GGYRCC	1 cut(s)	2651
BspTI	CTTAAG	2 cut(s)	1745, 2270
BspTNI	GGTCTC	1 cut(s)	367
BsrBI	CCGCTC	2 cut(s)	1143, 1634
BsrDI	GCAATG	4 cut(s)	375, 1680, 1956, 2529
BsrGI	TGTACA	1 cut(s)	1897
BsrI	ACTGG	6 cut(s)	651, 727, 820, 1067, 1246, 1792
BssECI	CCNNGG	6 cut(s)	962, 1014, 1530, 2204, 2403, 2575
BssNI	GRCGYC	2 cut(s)	5, 1429
BssSI	CACGAG	2 cut(s)	420, 2306
BssT1I	CCWWGG	1 cut(s)	2204
Bst2BI	CACGAG	2 cut(s)	420, 2306
Bst2UI	CCWGG	8 cut(s)	964, 1015, 1125, 1260, 1503, 1532, 1692, 2583
Bst4CI	ACNGT	6 cut(s)	199, 254, 1460, 1594, 1927, 2319
Bst6I	CTCTTC	3 cut(s)	854, 1150, 1746
BstACI	GRCGYC	2 cut(s)	5, 1429
BstAFI	CTTAAG	2 cut(s)	1745, 2270
BstAPI	GCANNNNNTGC	1 cut(s)	449
BstAUI	TGTACA	1 cut(s)	1897
BstBAI	YACGTR	1 cut(s)	1055
BstC8I	GCNNGC	5 cut(s)	1557, 1963, 2181, 2478, 2637
BstDSI	CCRYGG	1 cut(s)	2575
BstENI	CCTNNNNNAGG	1 cut(s)	1535
BstF5I	GGATG	7 cut(s)	138, 693, 1048, 1083, 1165, 2215, 2413
BstFNI	CGCG	2 cut(s)	96, 2577
BstH2I	RGCGCY	1 cut(s)	118
BstHHI	GCGC	2 cut(s)	98, 117
BstMAI	GTCTC	5 cut(s)	148, 367, 417, 760, 1808
BstMWI	GCNNNNNNNGC	7 cut(s)	135, 449, 584, 1421, 1631, 2180, 2408
BstNI	CCWGG	8 cut(s)	964, 1015, 1125, 1260, 1503, 1532, 1692, 2583
BstNSI	RCATGY	2 cut(s)	1898, 2000
BstSCI	CCNGG	9 cut(s)	962, 1013, 1123, 1258, 1359, 1501, 1530, 1690, 2581
BstSFI	CTRYAG	5 cut(s)	882, 949, 1459, 1560, 1668
BstSLI	GKGCMC	3 cut(s)	281, 1627, 2656
BstUI	CGCG	2 cut(s)	96, 2577
BstV1I	GCAGC	6 cut(s)	427, 896, 1484, 1748, 1903, 2460
BstV2I	GAAGAC	3 cut(s)	998, 2149, 2608
BstX2I	RGATCY	1 cut(s)	69
BstXI	CCANNNNNNTGG	1 cut(s)	1973
BstYI	RGATCY	1 cut(s)	69
BsuI	GTATCC	1 cut(s)	685
BsuRI	GGCC	5 cut(s)	1013, 1220, 1482, 1625, 2032
BtgI	CCRYGG	1 cut(s)	2575
BtsCI	GGATG	7 cut(s)	138, 693, 1048, 1083, 1165, 2215, 2413
BtsI	GCAGTG	2 cut(s)	987, 1391
BtsIMutI	CAGTG	4 cut(s)	987, 1391, 2110, 2626
Cac8I	GCNNGC	5 cut(s)	1557, 1963, 2181, 2478, 2637
CaiI	CAGNNNCTG	2 cut(s)	646, 2588
CfoI	GCGC	2 cut(s)	98, 117
Cfr13I	GGNCC	8 cut(s)	958, 1168, 1219, 1623, 1624, 1688, 2030, 2579
Cfr42I	CCGCGG	1 cut(s)	2578
CseI	GACGC	1 cut(s)	1418
Csp6I	GTAC	8 cut(s)	394, 831, 1056, 1263, 1497, 1582, 1898, 2551
CviAII	CATG	8 cut(s)	584, 620, 892, 1895, 1901, 1997, 2350, 2375
CviQI	GTAC	8 cut(s)	394, 831, 1056, 1263, 1497, 1582, 1898, 2551
DriI	GACNNNNNGTC	1 cut(s)	256
EaeI	YGGCCR	1 cut(s)	1480
Eam1104I	CTCTTC	3 cut(s)	854, 1150, 1746
Eam1105I	GACNNNNNGTC	1 cut(s)	256
EarI	CTCTTC	3 cut(s)	854, 1150, 1746
Eco130I	CCWWGG	1 cut(s)	2204
Eco147I	AGGCCT	1 cut(s)	1013
Eco24I	GRGCYC	1 cut(s)	1627
Eco31I	GGTCTC	1 cut(s)	367
Eco32I	GATATC	2 cut(s)	1320, 2290
Eco47I	GGWCC	4 cut(s)	958, 1168, 1688, 2579
Eco47III	AGCGCT	1 cut(s)	116
Eco57I	CTGAAG	5 cut(s)	192, 1010, 2086, 2602, 2616
EcoNI	CCTNNNNNAGG	1 cut(s)	1535
EcoO109I	RGGNCCY	3 cut(s)	1623, 1624, 2030
EcoRI	GAATTC	2 cut(s)	2023, 2345
EcoRII	CCWGG	8 cut(s)	962, 1013, 1123, 1258, 1501, 1530, 1690, 2581
EcoRV	GATATC	2 cut(s)	1320, 2290
EcoT14I	CCWWGG	1 cut(s)	2204
EcoT38I	GRGCYC	1 cut(s)	1627
ErhI	CCWWGG	1 cut(s)	2204
Esp3I	CGTCTC	1 cut(s)	1808
FaeI	CATG	8 cut(s)	587, 623, 895, 1898, 1904, 2000, 2353, 2378
FalI	AAGNNNNNCTT	6 cut(s)	59, 91, 222, 254, 543, 575
FaqI	GGGAC	3 cut(s)	1343, 2087, 2122
FatI	CATG	8 cut(s)	583, 619, 891, 1894, 1900, 1996, 2349, 2374
FauI	CCCGC	1 cut(s)	1599
FbaI	TGATCA	3 cut(s)	730, 1717, 2038
FblI	GTMKAC	1 cut(s)	201
Fnu4HI	GCNGC	9 cut(s)	94, 441, 885, 1141, 1473, 1737, 1892, 2474, 2564
FokI	GGATG	7 cut(s)	145, 700, 1035, 1070, 1172, 2222, 2420
FriOI	GRGCYC	1 cut(s)	1627
Fsp4HI	GCNGC	9 cut(s)	94, 441, 885, 1141, 1473, 1737, 1892, 2474, 2564
FspBI	CTAG	9 cut(s)	828, 1269, 1329, 1401, 1644, 1833, 1931, 2063, 2547
GlaI	GCGC	2 cut(s)	97, 116
GluI	GCNGC	9 cut(s)	94, 441, 885, 1141, 1473, 1737, 1892, 2474, 2564
GsaI	CCCAGC	1 cut(s)	1428
GsuI	CTGGAG	1 cut(s)	1524
HaeII	RGCGCY	1 cut(s)	118
HaeIII	GGCC	5 cut(s)	1013, 1220, 1482, 1625, 2032
HapII	CCGG	1 cut(s)	1361
HgaI	GACGC	1 cut(s)	1418
HhaI	GCGC	2 cut(s)	98, 117
Hin1I	GRCGYC	2 cut(s)	5, 1429
Hin1II	CATG	8 cut(s)	587, 623, 895, 1898, 1904, 2000, 2353, 2378
Hin6I	GCGC	2 cut(s)	96, 115
HinP1I	GCGC	2 cut(s)	96, 115
HincII	GTYRAC	4 cut(s)	715, 1825, 1936, 2452
HindII	GTYRAC	4 cut(s)	715, 1825, 1936, 2452
HindIII	AAGCTT	1 cut(s)	1413
HinfI	GANTC	8 cut(s)	338, 593, 985, 1181, 1906, 1949, 2261, 2613
HpaII	CCGG	1 cut(s)	1361
HphI	GGTGA	3 cut(s)	260, 1996, 2486
Hpy166II	GTNNAC	9 cut(s)	202, 715, 833, 1168, 1639, 1825, 1936, 2190, 2452
Hpy188I	TCNGA	8 cut(s)	298, 343, 730, 760, 990, 1193, 1579, 2287
Hpy8I	GTNNAC	9 cut(s)	202, 715, 833, 1168, 1639, 1825, 1936, 2190, 2452
Hpy99I	CGWCG	3 cut(s)	10, 19, 22
HpyAV	CCTTC	5 cut(s)	1229, 1360, 2233, 2317, 2452
HpyCH4III	ACNGT	6 cut(s)	199, 254, 1460, 1594, 1927, 2319
HpyCH4IV	ACGT	4 cut(s)	1054, 1188, 1551, 2430
HpyF10VI	GCNNNNNNNGC	7 cut(s)	135, 449, 584, 1421, 1631, 2180, 2408
HpySE526I	ACGT	4 cut(s)	1054, 1188, 1551, 2430
Hsp92I	GRCGYC	2 cut(s)	5, 1429
Hsp92II	CATG	8 cut(s)	587, 623, 895, 1898, 1904, 2000, 2353, 2378
HspAI	GCGC	2 cut(s)	96, 115
Ksp22I	TGATCA	3 cut(s)	730, 1717, 2038
KspI	CCGCGG	1 cut(s)	2578
LguI	GCTCTTC	1 cut(s)	1150
LmnI	GCTCC	3 cut(s)	2064, 2194, 2585
Lsp1109I	GCAGC	6 cut(s)	427, 896, 1484, 1748, 1903, 2460
LweI	GCATC	7 cut(s)	197, 439, 659, 1057, 2106, 2342, 2398
MaeI	CTAG	9 cut(s)	828, 1269, 1329, 1401, 1644, 1833, 1931, 2063, 2547
MaeII	ACGT	4 cut(s)	1054, 1188, 1551, 2430
MbiI	CCGCTC	2 cut(s)	1143, 1634
MflI	RGATCY	1 cut(s)	69
MhlI	GDGCHC	4 cut(s)	281, 901, 1627, 2656
MlsI	TGGCCA	1 cut(s)	1482
MluNI	TGGCCA	1 cut(s)	1482
MlyI	GAGTC	3 cut(s)	347, 1943, 2622
MmeI	TCCRAC	2 cut(s)	321, 1666
Mox20I	TGGCCA	1 cut(s)	1482
MroXI	GAANNNNTTC	1 cut(s)	1106
MscI	TGGCCA	1 cut(s)	1482
MseI	TTAA	9 cut(s)	306, 407, 527, 1290, 1548, 1601, 1746, 1758, 2271
MslI	CAYNNNNRTG	3 cut(s)	284, 1899, 1971
Msp20I	TGGCCA	1 cut(s)	1482
MspA1I	CMGCKG	6 cut(s)	479, 1475, 1946, 2339, 2577, 2606
MspCI	CTTAAG	2 cut(s)	1745, 2270
MspI	CCGG	1 cut(s)	1361
MspR9I	CCNGG	9 cut(s)	964, 1015, 1125, 1260, 1361, 1503, 1532, 1692, 2583
Mva1269I	GAATGC	1 cut(s)	1426
MvaI	CCWGG	8 cut(s)	964, 1015, 1125, 1260, 1503, 1532, 1692, 2583
MvnI	CGCG	2 cut(s)	96, 2577
MwoI	GCNNNNNNNGC	7 cut(s)	135, 449, 584, 1421, 1631, 2180, 2408
NciI	CCSGG	1 cut(s)	1361
NlaIII	CATG	8 cut(s)	587, 623, 895, 1898, 1904, 2000, 2353, 2378
NlaIV	GGNNCC	6 cut(s)	960, 1406, 1625, 2060, 2196, 2653
NmeAIII	GCCGAG	1 cut(s)	2428
NmuCI	GTSAC	5 cut(s)	193, 248, 872, 2002, 2621
NspI	RCATGY	2 cut(s)	1898, 2000
OliI	CACNNNNGTG	1 cut(s)	284
PceI	AGGCCT	1 cut(s)	1013
PciI	ACATGT	1 cut(s)	1996
PciSI	GCTCTTC	1 cut(s)	1150
PcsI	WCGNNNNNNNCGW	2 cut(s)	14, 20
PctI	GAATGC	1 cut(s)	1426
PdmI	GAANNNNTTC	1 cut(s)	1106
PfeI	GAWTC	5 cut(s)	593, 985, 1181, 1906, 2261
PflFI	GACNNNGTC	2 cut(s)	997, 1952
PflMI	CCANNNNNTGG	4 cut(s)	1177, 1489, 2380, 2588
PfoI	TCCNGGA	2 cut(s)	1359, 1501
PkrI	GCNGC	9 cut(s)	95, 442, 886, 1142, 1474, 1738, 1893, 2475, 2565
PleI	GAGTC	3 cut(s)	346, 1943, 2621
PpsI	GAGTC	3 cut(s)	346, 1943, 2621
Ppu21I	YACGTR	1 cut(s)	1055
PscI	ACATGT	1 cut(s)	1996
PshBI	ATTAAT	1 cut(s)	306
Psp6I	CCWGG	8 cut(s)	962, 1013, 1123, 1258, 1501, 1530, 1690, 2581
PspFI	CCCAGC	1 cut(s)	1424
PspGI	CCWGG	8 cut(s)	962, 1013, 1123, 1258, 1501, 1530, 1690, 2581
PspN4I	GGNNCC	6 cut(s)	960, 1406, 1625, 2060, 2196, 2653
PspOMI	GGGCCC	1 cut(s)	1623
PspPI	GGNCC	8 cut(s)	958, 1168, 1219, 1623, 1624, 1688, 2030, 2579
PstI	CTGCAG	1 cut(s)	886
PstNI	CAGNNNCTG	2 cut(s)	646, 2588
PsuI	RGATCY	1 cut(s)	69
PsyI	GACNNNGTC	2 cut(s)	997, 1952
PvuII	CAGCTG	4 cut(s)	479, 1946, 2339, 2606
RsaI	GTAC	8 cut(s)	395, 832, 1057, 1264, 1498, 1583, 1899, 2552
RsaNI	GTAC	8 cut(s)	394, 831, 1056, 1263, 1497, 1582, 1898, 2551
RseI	CAYNNNNRTG	3 cut(s)	284, 1899, 1971
SacII	CCGCGG	1 cut(s)	2578
SapI	GCTCTTC	1 cut(s)	1150
SaqAI	TTAA	9 cut(s)	306, 407, 527, 1290, 1548, 1601, 1746, 1758, 2271
SatI	GCNGC	9 cut(s)	94, 441, 885, 1141, 1473, 1737, 1892, 2474, 2564
Sau96I	GGNCC	8 cut(s)	958, 1168, 1219, 1623, 1624, 1688, 2030, 2579
ScaI	AGTACT	1 cut(s)	1498
SchI	GAGTC	3 cut(s)	347, 1943, 2622
ScrFI	CCNGG	9 cut(s)	964, 1015, 1125, 1260, 1361, 1503, 1532, 1692, 2583
SduI	GDGCHC	4 cut(s)	281, 901, 1627, 2656
SfaNI	GCATC	7 cut(s)	197, 439, 659, 1057, 2106, 2342, 2398
SfcI	CTRYAG	5 cut(s)	882, 949, 1459, 1560, 1668
Sfr303I	CCGCGG	1 cut(s)	2578
SgrBI	CCGCGG	1 cut(s)	2578
SinI	GGWCC	4 cut(s)	958, 1168, 1688, 2579
SmiMI	CAYNNNNRTG	3 cut(s)	284, 1899, 1971
SmlI	CTYRAG	6 cut(s)	770, 1566, 1745, 1792, 2270, 2422
SmoI	CTYRAG	6 cut(s)	770, 1566, 1745, 1792, 2270, 2422
SseBI	AGGCCT	1 cut(s)	1013
SspI	AATATT	2 cut(s)	1546, 2082
SspMI	CTAG	9 cut(s)	828, 1269, 1329, 1401, 1644, 1833, 1931, 2063, 2547
StuI	AGGCCT	1 cut(s)	1013
StyD4I	CCNGG	9 cut(s)	962, 1013, 1123, 1258, 1359, 1501, 1530, 1690, 2581
StyI	CCWWGG	1 cut(s)	2204
TaaI	ACNGT	6 cut(s)	199, 254, 1460, 1594, 1927, 2319
TaiI	ACGT	4 cut(s)	1057, 1191, 1554, 2433
TaqI	TCGA	4 cut(s)	483, 738, 750, 2370
TatI	WGTACW	4 cut(s)	830, 1496, 1581, 1897
TauI	GCSGC	3 cut(s)	96, 1143, 2566
TfiI	GAWTC	5 cut(s)	593, 985, 1181, 1906, 2261
Tru1I	TTAA	9 cut(s)	306, 407, 527, 1290, 1548, 1601, 1746, 1758, 2271
Tru9I	TTAA	9 cut(s)	306, 407, 527, 1290, 1548, 1601, 1746, 1758, 2271
TscAI	CASTG	4 cut(s)	987, 1398, 2110, 2626
TseFI	GTSAC	5 cut(s)	193, 248, 872, 2002, 2621
TseI	GCWGC	6 cut(s)	440, 884, 1472, 1736, 1891, 2473
Tsp45I	GTSAC	5 cut(s)	193, 248, 872, 2002, 2621
TspDTI	ATGAA	5 cut(s)	254, 516, 1176, 2338, 2631
TspGWI	ACGGA	2 cut(s)	1397, 2207
TspRI	CASTG	4 cut(s)	987, 1398, 2110, 2626
Tth111I	GACNNNGTC	2 cut(s)	997, 1952
Van91I	CCANNNNNTGG	4 cut(s)	1177, 1489, 2380, 2588
Vha464I	CTTAAG	2 cut(s)	1745, 2270
VpaK11BI	GGWCC	4 cut(s)	958, 1168, 1688, 2579
VspI	ATTAAT	1 cut(s)	306
XagI	CCTNNNNNAGG	1 cut(s)	1535
XapI	RAATTY	5 cut(s)	269, 1134, 1883, 2023, 2345
XbaI	TCTAGA	2 cut(s)	1643, 1832
XceI	RCATGY	2 cut(s)	1898, 2000
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1672
XmiI	GTMKAC	1 cut(s)	201
XmnI	GAANNNNTTC	1 cut(s)	1106
XspI	CTAG	9 cut(s)	828, 1269, 1329, 1401, 1644, 1833, 1931, 2063, 2547
ZrmI	AGTACT	1 cut(s)	1498

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.