Rmu_sc0001863.1_g000016

E3 ubiquitin-protein ligase RNF170-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001863.1
OG0007874
Physical Location & Seq
Forward (+)
60875 .. 65895
5021 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 597 bp
atgcttgtttgggatcatggatcaacacttcagccttgtaaatgccctctctgccgtcgccagataaccttgctggttccaactgaggcttcattgagccaacgtgatatgcctgaggtggctgagattctaggcaaggttgaaacatataatcgctatttcggaggtcaatccagaggtcttattcagagaatgcaagaccttccttttcttttgcggaggctgttacgagaactgttggatccccatagatcgcttccccttgttataagggctcgtgtcattattgcaatgatcctaagcggtgcctatgtactcagtcctgttgactttattccagaagcaatatttggtatagtcggtctatttgatgatctcctcatagtactcgtggtcttcctccacgttgctaccatttatcggtcggttccaaaggacctgctttctcttctctttcttagcgctccgccccctgaattgactgtcaaggaggcagagatcgatcaggctctccaaaagttggtcgttgttaaggagaatggagataaccccaactctgagaagaattcctttcaaatcaataagagattgatctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1232 zf-C3HC4 zf-C3HC4_3

Protein Analysis

198

Amino Acids

22.35

Weight (kDa)

7.73

Isoelectric Point (pI)

50.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1232 PF06803 95 - 130 4.3e-08 Protein of unknown function (DUF1232)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 269
Acc36I ACCTGC 1 cut(s) 447
AccB1I GGYRCC 1 cut(s) 305
AccB7I CCANNNNNTGG 1 cut(s) 520
AciI CCGC 3 cut(s) 217, 303, 467
AclWI GGATC 5 cut(s) 21, 28, 236, 249, 289
AcsI RAATTY 1 cut(s) 565
AcuI CTGAAG 1 cut(s) 14
AfaI GTAC 2 cut(s) 315, 387
AfeI AGCGCT 1 cut(s) 463
AfiI CCNNNNNNNGG 2 cut(s) 420, 520
AgsI TTSAA 2 cut(s) 143, 575
AjuI GAANNNNNNNTTGG 4 cut(s) 73, 105, 333, 365
AlwI GGATC 5 cut(s) 21, 28, 236, 249, 289
Aor51HI AGCGCT 1 cut(s) 463
ApoI RAATTY 1 cut(s) 565
AspLEI GCGC 1 cut(s) 464
AspS9I GGNCC 1 cut(s) 436
AvaII GGWCC 1 cut(s) 436
AxyI CCTNAGG 1 cut(s) 114
BamHI GGATCC 1 cut(s) 241
BanI GGYRCC 1 cut(s) 305
BanII GRGCYC 1 cut(s) 277
BauI CACGAG 2 cut(s) 276, 389
BbsI GAAGAC 1 cut(s) 388
BceAI ACGGC 1 cut(s) 39
BfaI CTAG 1 cut(s) 131
BfoI RGCGCY 1 cut(s) 465
BfuAI ACCTGC 1 cut(s) 447
BmcAI AGTACT 1 cut(s) 387
Bme18I GGWCC 1 cut(s) 436
BmgT120I GGNCC 1 cut(s) 436
BmiI GGNNCC 4 cut(s) 78, 243, 307, 429
BpiI GAAGAC 1 cut(s) 388
Bpu10I CCTNAGC 1 cut(s) 299
Bsa29I ATCGAT 1 cut(s) 501
Bsc4I CCNNNNNNNGG 2 cut(s) 420, 520
Bse21I CCTNAGG 1 cut(s) 114
Bse3DI GCAATG 1 cut(s) 297
BseCI ATCGAT 1 cut(s) 501
BseLI CCNNNNNNNGG 2 cut(s) 420, 520
BseMI GCAATG 1 cut(s) 297
BseMII CTCAG 5 cut(s) 75, 105, 114, 331, 549
BseRI GAGGAG 1 cut(s) 368
Bsh1285I CGRYCG 1 cut(s) 425
BshNI GGYRCC 1 cut(s) 305
BshVI ATCGAT 1 cut(s) 501
BsiEI CGRYCG 1 cut(s) 425
BslI CCNNNNNNNGG 2 cut(s) 420, 520
BsmI GAATGC 1 cut(s) 198
Bsp1286I GDGCHC 1 cut(s) 277
Bsp143I GATC 9 cut(s) 13, 20, 241, 251, 294, 373, 498, 502, 591
BspACI CCGC 3 cut(s) 217, 303, 467
BspCNI CTCAG 5 cut(s) 76, 106, 115, 330, 550
BspDI ATCGAT 1 cut(s) 501
BspLI GGNNCC 4 cut(s) 78, 243, 307, 429
BspMI ACCTGC 1 cut(s) 447
BspPI GGATC 5 cut(s) 21, 28, 236, 249, 289
BspT107I GGYRCC 1 cut(s) 305
BsrDI GCAATG 1 cut(s) 297
BssMI GATC 9 cut(s) 13, 20, 241, 251, 294, 373, 498, 502, 591
BssSI CACGAG 2 cut(s) 276, 389
Bst2BI CACGAG 2 cut(s) 276, 389
Bst4CI ACNGT 2 cut(s) 237, 484
Bst6I CTCTTC 1 cut(s) 453
BstDEI CTNAG 7 cut(s) 84, 114, 123, 299, 317, 458, 558
BstH2I RGCGCY 1 cut(s) 465
BstHHI GCGC 1 cut(s) 464
BstKTI GATC 9 cut(s) 16, 23, 244, 254, 297, 376, 501, 505, 594
BstMBI GATC 9 cut(s) 13, 20, 241, 251, 294, 373, 498, 502, 591
BstMCI CGRYCG 1 cut(s) 425
BstMWI GCNNNNNNNGC 1 cut(s) 51
BstV2I GAAGAC 1 cut(s) 388
BstX2I RGATCY 1 cut(s) 241
BstYI RGATCY 1 cut(s) 241
Bsu15I ATCGAT 1 cut(s) 501
Bsu36I CCTNAGG 1 cut(s) 114
BsuTUI ATCGAT 1 cut(s) 501
BveI ACCTGC 1 cut(s) 447
CfoI GCGC 1 cut(s) 464
Cfr13I GGNCC 1 cut(s) 436
ClaI ATCGAT 1 cut(s) 501
Csp6I GTAC 2 cut(s) 314, 386
CviAII CATG 1 cut(s) 17
CviJI RGCY 7 cut(s) 34, 89, 99, 122, 223, 275, 509
CviKI_1 RGCY 7 cut(s) 34, 89, 99, 122, 223, 275, 509
CviQI GTAC 2 cut(s) 314, 386
DdeI CTNAG 7 cut(s) 84, 114, 123, 299, 317, 458, 558
DpnI GATC 9 cut(s) 15, 22, 243, 253, 296, 375, 500, 504, 593
DpnII GATC 9 cut(s) 13, 20, 241, 251, 294, 373, 498, 502, 591
Eam1104I CTCTTC 1 cut(s) 453
EarI CTCTTC 1 cut(s) 453
EciI GGCGGA 1 cut(s) 456
Eco24I GRGCYC 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 436
Eco47III AGCGCT 1 cut(s) 463
Eco57I CTGAAG 1 cut(s) 14
Eco81I CCTNAGG 1 cut(s) 114
EcoO109I RGGNCCY 1 cut(s) 436
EcoRI GAATTC 1 cut(s) 565
EcoT38I GRGCYC 1 cut(s) 277
FaeI CATG 1 cut(s) 20
FaiI YATR 9 cut(s) 18, 110, 148, 150, 249, 269, 312, 356, 383
FalI AAGNNNNNCTT 2 cut(s) 554, 586
FatI CATG 1 cut(s) 16
FriOI GRGCYC 1 cut(s) 277
FspBI CTAG 1 cut(s) 131
GlaI GCGC 1 cut(s) 463
HaeII RGCGCY 1 cut(s) 465
HhaI GCGC 1 cut(s) 464
Hin1II CATG 1 cut(s) 20
Hin6I GCGC 1 cut(s) 462
HinP1I GCGC 1 cut(s) 462
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HinfI GANTC 1 cut(s) 127
Hpy166II GTNNAC 1 cut(s) 328
Hpy188I TCNGA 4 cut(s) 164, 189, 559, 596
Hpy188III TCNNGA 2 cut(s) 174, 338
Hpy8I GTNNAC 1 cut(s) 328
Hpy99I CGWCG 1 cut(s) 60
HpyAV CCTTC 1 cut(s) 212
HpyCH4III ACNGT 2 cut(s) 237, 484
HpyCH4IV ACGT 2 cut(s) 103, 405
HpyCH4V TGCA 2 cut(s) 196, 290
HpyF10VI GCNNNNNNNGC 1 cut(s) 51
HpyF3I CTNAG 7 cut(s) 84, 114, 123, 299, 317, 458, 558
HpySE526I ACGT 2 cut(s) 103, 405
Hsp92II CATG 1 cut(s) 20
HspAI GCGC 1 cut(s) 462
Kzo9I GATC 9 cut(s) 13, 20, 241, 251, 294, 373, 498, 502, 591
LmnI GCTCC 1 cut(s) 469
LpnPI CCDG 9 cut(s) 59, 74, 126, 187, 336, 351, 452, 486, 491
MaeI CTAG 1 cut(s) 131
MaeII ACGT 2 cut(s) 103, 405
MaeIII GTNAC 1 cut(s) 225
MalI GATC 9 cut(s) 15, 22, 243, 253, 296, 375, 500, 504, 593
MboI GATC 9 cut(s) 13, 20, 241, 251, 294, 373, 498, 502, 591
MboII GAAGA 3 cut(s) 388, 440, 574
MflI RGATCY 1 cut(s) 241
MhlI GDGCHC 1 cut(s) 277
MluCI AATT 2 cut(s) 476, 565
MmeI TCCRAC 2 cut(s) 104, 219
MnlI CCTC 9 cut(s) 57, 79, 109, 158, 170, 213, 389, 410, 484
MseI TTAA 1 cut(s) 531
Mva1269I GAATGC 1 cut(s) 198
MwoI GCNNNNNNNGC 1 cut(s) 51
NdeII GATC 9 cut(s) 13, 20, 241, 251, 294, 373, 498, 502, 591
NlaIII CATG 1 cut(s) 20
NlaIV GGNNCC 4 cut(s) 78, 243, 307, 429
PctI GAATGC 1 cut(s) 198
PfeI GAWTC 1 cut(s) 127
PflMI CCANNNNNTGG 1 cut(s) 520
PpuMI RGGWCCY 1 cut(s) 436
PsiI TTATAA 1 cut(s) 269
Psp5II RGGWCCY 1 cut(s) 436
PspN4I GGNNCC 4 cut(s) 78, 243, 307, 429
PspPI GGNCC 1 cut(s) 436
PspPPI RGGWCCY 1 cut(s) 436
PsuI RGATCY 1 cut(s) 241
RsaI GTAC 2 cut(s) 315, 387
RsaNI GTAC 2 cut(s) 314, 386
SaqAI TTAA 1 cut(s) 531
Sau3AI GATC 9 cut(s) 13, 20, 241, 251, 294, 373, 498, 502, 591
Sau96I GGNCC 1 cut(s) 436
ScaI AGTACT 1 cut(s) 387
SduI GDGCHC 1 cut(s) 277
SetI ASST 9 cut(s) 71, 106, 120, 141, 169, 181, 204, 408, 441
SinI GGWCC 1 cut(s) 436
Sse9I AATT 2 cut(s) 476, 565
SsiI CCGC 3 cut(s) 217, 303, 467
SspI AATATT 1 cut(s) 348
SspMI CTAG 1 cut(s) 131
TaaI ACNGT 2 cut(s) 237, 484
TaiI ACGT 2 cut(s) 106, 408
TaqI TCGA 1 cut(s) 501
TaqII GACCGA 2 cut(s) 350, 411
TasI AATT 2 cut(s) 476, 565
TatI WGTACW 2 cut(s) 313, 385
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TspDTI ATGAA 1 cut(s) 81
Van91I CCANNNNNTGG 1 cut(s) 520
VpaK11BI GGWCC 1 cut(s) 436
XapI RAATTY 1 cut(s) 565
XspI CTAG 1 cut(s) 131
ZrmI AGTACT 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.