Rmu_sc0002040.1_g000074

Kinetochore protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002040.1
OG0007257
Physical Location & Seq
Reverse (-)
316975 .. 321306
4332 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1389 bp
atggacaccaaaccagaagaatccgtacggtcgaaaatggagtctctaaagctggcctgcgacagagagattccggctcacctgcagaaggcggacgctgccgcggcggcgcttcgcgactccctggcttcgatccgcgccagagtacaagaaaccgtccactgccaagggttttcggatccggcgatggacaccaaaccagaagaatccgtacggtcgaaaatggagtctctaaagctggcctgcgacagagagattccggctcacctgcagaaggcggacgctgccgcggcggcgcttcgcgactccctggcttcgatccgcgccagagtacaagaaaccgtccactgccaagggaaactcggacaggttaaagctaaactgagggaagccgaggaggaattgtttaaagccctagcagcaaagactcgtaaagagaccaagcggatggcaatgacggatgcgattgctgccaggaaagctagaatagacgaacttaagagagtggtgcaagatcgaagggctaagagggatgagtatggtgcaattctttctcagcaattttctgaatctgaagataggtctatcgaagacagtaaagaagaaatagaagaggttatatcatggtataataaggtccttggtttctatattgaaggcggacatggggtgagattcacatttaagaacatcaacttgaaaaatccagatgaggaattctgcttcactgttaggcatgcaaatgatgtgtacaccttgatagattgtgatccacacttgaatgagatcaaagagttgttagacgaattgaacagaaccaatggattatttaaatttgtcagagatatgagacaaaggtttcaagaagctgcctctcgagggttaccaatggttatgcatcaagagtattccacaatttctgggtctgctccagctttgtcaatgtcaactgtcagaagtgaatcttttgccaagagtcatgagcatcaccaagttcaacatgaaaaacctgatagacaagtcaagaaacttagccctggaaagtggaataaatcacaaagtctatctcctgggtcggcattgtctcttcgtcgctctcctcgttttaagtgcctgcaagctctgcttgccttctgccctcgcacccgctcgcctgcccttgcaaccgcgactcaccagcgacaccgcgaccctgctaccccagtgtcaccgcaactcgcccgtgacaccgcaatatcgcactcctacgaccctagcgacaccgtgactctgcgaccctcgcgactccgcagtcccacgacccctgcgcctcctcattcctgtgactcgccctcgaccgcgccactattgcgactccgcgaccgccatctcattagaatgctcgcctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spindle_Spc25

Protein Analysis

462

Amino Acids

52.03

Weight (kDa)

9.22

Isoelectric Point (pI)

66.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spindle_Spc25 PF08234 220 - 288 6.4e-23 Chromosome segregation protein Spc25
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 90, 276
AasI GACNNNNNNGTC 1 cut(s) 1290
Acc36I ACCTGC 2 cut(s) 90, 276
AccBSI CCGCTC 1 cut(s) 1149
AclWI GGATC 5 cut(s) 127, 173, 186, 313, 764
AcsI RAATTY 2 cut(s) 716, 833
AcuI CTGAAG 1 cut(s) 594
AfaI GTAC 5 cut(s) 27, 147, 213, 333, 752
AfiI CCNNNNNNNGG 3 cut(s) 88, 274, 879
AflII CTTAAG 1 cut(s) 497
AgsI TTSAA 6 cut(s) 656, 700, 781, 811, 863, 998
AjnI CCWGG 5 cut(s) 123, 309, 473, 1036, 1069
AluBI AGCT 7 cut(s) 52, 238, 377, 482, 869, 935, 1121
AluI AGCT 7 cut(s) 52, 238, 377, 482, 869, 935, 1121
Alw26I GTCTC 5 cut(s) 48, 234, 431, 844, 1089
AlwI GGATC 5 cut(s) 127, 173, 186, 313, 764
Ama87I CYCGRG 1 cut(s) 876
AoxI GGCC 2 cut(s) 54, 240
ApeKI GCWGC 5 cut(s) 98, 284, 419, 470, 869
ApoI RAATTY 2 cut(s) 716, 833
ArsI GACNNNNNNTTYG 4 cut(s) 26, 58, 212, 244
AspLEI GCGC 6 cut(s) 112, 140, 298, 326, 1309, 1342
AspS9I GGNCC 1 cut(s) 637
AsuHPI GGTGA 6 cut(s) 71, 257, 682, 980, 1166, 1200
AvaI CYCGRG 1 cut(s) 876
AvaII GGWCC 1 cut(s) 637
BamHI GGATCC 1 cut(s) 178
BarI GAAGNNNNNNTAC 4 cut(s) 9, 41, 195, 227
BbsI GAAGAC 1 cut(s) 597
BbvI GCAGC 5 cut(s) 85, 271, 431, 457, 856
BccI CCATC 3 cut(s) 181, 442, 1374
BciT130I CCWGG 5 cut(s) 125, 311, 475, 1038, 1071
BcoDI GTCTC 5 cut(s) 48, 234, 431, 844, 1089
BfaI CTAG 4 cut(s) 416, 483, 1254, 1387
BfmI CTRYAG 2 cut(s) 83, 269
BfoI RGCGCY 2 cut(s) 113, 299
BfrI CTTAAG 1 cut(s) 497
BfuAI ACCTGC 2 cut(s) 90, 276
BglI GCCNNNNNGGC 2 cut(s) 107, 293
Bme1390I CCNGG 5 cut(s) 125, 311, 475, 1038, 1071
Bme18I GGWCC 1 cut(s) 637
BmeT110I CYCGRG 1 cut(s) 876
BmgT120I GGNCC 1 cut(s) 637
BmiI GGNNCC 1 cut(s) 180
BmrFI CCNGG 5 cut(s) 125, 311, 475, 1038, 1071
BmrI ACTGGG 1 cut(s) 1196
BmsI GCATC 3 cut(s) 451, 907, 994
BmuI ACTGGG 1 cut(s) 1196
BpiI GAAGAC 1 cut(s) 597
BpmI CTGGAG 1 cut(s) 915
BsaBI GATNNNNATC 1 cut(s) 768
BsaI GGTCTC 1 cut(s) 431
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 274, 879
Bse1I ACTGG 1 cut(s) 1202
Bse3DI GCAATG 1 cut(s) 459
Bse8I GATNNNNATC 1 cut(s) 768
BseBI CCWGG 5 cut(s) 125, 311, 475, 1038, 1071
BseGI GGATG 3 cut(s) 453, 466, 538
BseJI GATNNNNATC 1 cut(s) 768
BseLI CCNNNNNNNGG 3 cut(s) 88, 274, 879
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 2 cut(s) 374, 569
BseNI ACTGG 1 cut(s) 1202
BseRI GAGGAG 3 cut(s) 410, 1089, 1302
BseXI GCAGC 5 cut(s) 85, 271, 431, 457, 856
Bsh1285I CGRYCG 4 cut(s) 32, 218, 1338, 1363
BshFI GGCC 2 cut(s) 56, 242
BsiEI CGRYCG 4 cut(s) 32, 218, 1338, 1363
BsiHKCI CYCGRG 1 cut(s) 876
BsiSI CCGG 3 cut(s) 74, 182, 260
BsiWI CGTACG 2 cut(s) 25, 211
BslFI GGGAC 1 cut(s) 1278
BslI CCNNNNNNNGG 3 cut(s) 88, 274, 879
BsmAI GTCTC 5 cut(s) 48, 234, 431, 844, 1089
BsmFI GGGAC 1 cut(s) 1278
BsmI GAATGC 1 cut(s) 1383
BsnI GGCC 2 cut(s) 56, 242
Bso31I GGTCTC 1 cut(s) 431
BsoBI CYCGRG 1 cut(s) 876
Bsp1407I TGTACA 1 cut(s) 750
Bsp143I GATC 6 cut(s) 132, 178, 318, 514, 769, 786
Bsp68I TCGCGA 3 cut(s) 117, 303, 1282
BspANI GGCC 2 cut(s) 56, 242
BspCNI CTCAG 2 cut(s) 375, 568
BspHI TCATGA 1 cut(s) 979
BspLI GGNNCC 1 cut(s) 180
BspMAI CTGCAG 2 cut(s) 87, 273
BspMI ACCTGC 2 cut(s) 90, 276
BspPI GGATC 5 cut(s) 127, 173, 186, 313, 764
BspTI CTTAAG 1 cut(s) 497
BspTNI GGTCTC 1 cut(s) 431
BsrBI CCGCTC 1 cut(s) 1149
BsrDI GCAATG 1 cut(s) 459
BsrGI TGTACA 1 cut(s) 750
BsrI ACTGG 1 cut(s) 1202
BssMI GATC 6 cut(s) 132, 178, 318, 514, 769, 786
BssT1I CCWWGG 3 cut(s) 166, 352, 640
Bst2UI CCWGG 5 cut(s) 125, 311, 475, 1038, 1071
Bst4CI ACNGT 8 cut(s) 30, 157, 216, 343, 596, 730, 952, 1264
Bst6I CTCTTC 2 cut(s) 606, 1092
BstAFI CTTAAG 1 cut(s) 497
BstAPI GCANNNNNTGC 1 cut(s) 1123
BstAUI TGTACA 1 cut(s) 750
BstDEI CTNAG 4 cut(s) 383, 525, 555, 1031
BstDSI CCRYGG 2 cut(s) 102, 288
BstEII GGTNACC 1 cut(s) 882
BstENI CCTNNNNNAGG 2 cut(s) 86, 272
BstF5I GGATG 3 cut(s) 453, 466, 538
BstH2I RGCGCY 2 cut(s) 113, 299
BstHHI GCGC 6 cut(s) 112, 140, 298, 326, 1309, 1342
BstKTI GATC 6 cut(s) 135, 181, 321, 517, 772, 789
BstMAI GTCTC 5 cut(s) 48, 234, 431, 844, 1089
BstMBI GATC 6 cut(s) 132, 178, 318, 514, 769, 786
BstMCI CGRYCG 4 cut(s) 32, 218, 1338, 1363
BstNI CCWGG 5 cut(s) 125, 311, 475, 1038, 1071
BstNSI RCATGY 1 cut(s) 740
BstPI GGTNACC 1 cut(s) 882
BstSCI CCNGG 5 cut(s) 123, 309, 473, 1036, 1069
BstSFI CTRYAG 2 cut(s) 83, 269
BstV1I GCAGC 5 cut(s) 85, 271, 431, 457, 856
BstV2I GAAGAC 1 cut(s) 597
BstX2I RGATCY 1 cut(s) 178
BstXI CCANNNNNNTGG 1 cut(s) 448
BstYI RGATCY 1 cut(s) 178
BsuRI GGCC 2 cut(s) 56, 242
BtgI CCRYGG 2 cut(s) 102, 288
BtgZI GCGATG 1 cut(s) 200
BtsCI GGATG 3 cut(s) 453, 466, 538
BtsI GCAGTG 2 cut(s) 160, 346
BtsIMutI CAGTG 4 cut(s) 160, 346, 726, 1209
BtuMI TCGCGA 3 cut(s) 117, 303, 1282
BveI ACCTGC 2 cut(s) 90, 276
CciI TCATGA 1 cut(s) 979
CfoI GCGC 6 cut(s) 112, 140, 298, 326, 1309, 1342
Cfr13I GGNCC 1 cut(s) 637
Cfr42I CCGCGG 2 cut(s) 105, 291
CseI GACGC 2 cut(s) 104, 290
Csp6I GTAC 5 cut(s) 26, 146, 212, 332, 751
CviAII CATG 5 cut(s) 624, 665, 737, 980, 1001
CviQI GTAC 5 cut(s) 26, 146, 212, 332, 751
DdeI CTNAG 4 cut(s) 383, 525, 555, 1031
DpnI GATC 6 cut(s) 134, 180, 320, 516, 771, 788
DpnII GATC 6 cut(s) 132, 178, 318, 514, 769, 786
DraI TTTAAA 2 cut(s) 409, 832
DrdI GACNNNNNNGTC 1 cut(s) 1290
DseDI GACNNNNNNGTC 1 cut(s) 1290
Eam1104I CTCTTC 2 cut(s) 606, 1092
EarI CTCTTC 2 cut(s) 606, 1092
EciI GGCGGA 3 cut(s) 107, 293, 675
Eco130I CCWWGG 3 cut(s) 166, 352, 640
Eco31I GGTCTC 1 cut(s) 431
Eco47I GGWCC 1 cut(s) 637
Eco57I CTGAAG 1 cut(s) 594
Eco88I CYCGRG 1 cut(s) 876
Eco91I GGTNACC 1 cut(s) 882
EcoNI CCTNNNNNAGG 2 cut(s) 86, 272
EcoO109I RGGNCCY 1 cut(s) 637
EcoO65I GGTNACC 1 cut(s) 882
EcoRI GAATTC 1 cut(s) 716
EcoRII CCWGG 5 cut(s) 123, 309, 473, 1036, 1069
EcoT14I CCWWGG 3 cut(s) 166, 352, 640
EcoT22I ATGCAT 1 cut(s) 900
ErhI CCWWGG 3 cut(s) 166, 352, 640
FaeI CATG 5 cut(s) 627, 668, 740, 983, 1004
FalI AAGNNNNNCTT 4 cut(s) 949, 981, 1110, 1142
FaqI GGGAC 1 cut(s) 1278
FatI CATG 5 cut(s) 623, 664, 736, 979, 1000
FauI CCCGC 1 cut(s) 1154
FokI GGATG 3 cut(s) 460, 473, 545
FspBI CTAG 4 cut(s) 416, 483, 1254, 1387
GlaI GCGC 6 cut(s) 111, 139, 297, 325, 1308, 1341
GsuI CTGGAG 1 cut(s) 915
HaeII RGCGCY 2 cut(s) 113, 299
HaeIII GGCC 2 cut(s) 56, 242
HapII CCGG 3 cut(s) 74, 182, 260
HgaI GACGC 2 cut(s) 104, 290
HhaI GCGC 6 cut(s) 112, 140, 298, 326, 1309, 1342
Hin1II CATG 5 cut(s) 627, 668, 740, 983, 1004
Hin6I GCGC 6 cut(s) 110, 138, 296, 324, 1307, 1340
HinP1I GCGC 6 cut(s) 110, 138, 296, 324, 1307, 1340
HincII GTYRAC 1 cut(s) 948
HindII GTYRAC 1 cut(s) 948
HpaII CCGG 3 cut(s) 74, 182, 260
HphI GGTGA 6 cut(s) 71, 257, 682, 980, 1166, 1200
Hpy166II GTNNAC 5 cut(s) 160, 346, 751, 753, 948
Hpy188I TCNGA 6 cut(s) 178, 365, 568, 574, 842, 956
Hpy188III TCNNGA 9 cut(s) 116, 302, 707, 863, 876, 902, 980, 1024, 1281
Hpy8I GTNNAC 5 cut(s) 160, 346, 751, 753, 948
Hpy99I CGWCG 1 cut(s) 1095
HpyAV CCTTC 5 cut(s) 82, 268, 513, 650, 1141
HpyCH4III ACNGT 8 cut(s) 30, 157, 216, 343, 596, 730, 952, 1264
HpyCH4V TGCA 8 cut(s) 85, 271, 511, 545, 740, 898, 1117, 1163
HpyF3I CTNAG 4 cut(s) 383, 525, 555, 1031
Hsp92II CATG 5 cut(s) 627, 668, 740, 983, 1004
HspAI GCGC 6 cut(s) 110, 138, 296, 324, 1307, 1340
KspI CCGCGG 2 cut(s) 105, 291
Kzo9I GATC 6 cut(s) 132, 178, 318, 514, 769, 786
LmnI GCTCC 1 cut(s) 934
Lsp1109I GCAGC 5 cut(s) 85, 271, 431, 457, 856
LweI GCATC 3 cut(s) 451, 907, 994
MaeI CTAG 4 cut(s) 416, 483, 1254, 1387
MaeIII GTNAC 5 cut(s) 882, 1206, 1223, 1264, 1322
MalI GATC 6 cut(s) 134, 180, 320, 516, 771, 788
MbiI CCGCTC 1 cut(s) 1149
MboI GATC 6 cut(s) 132, 178, 318, 514, 769, 786
MboII GAAGA 7 cut(s) 29, 215, 587, 602, 614, 623, 1079
MflI RGATCY 1 cut(s) 178
MluCI AATT 7 cut(s) 401, 546, 560, 716, 806, 833, 915
Mph1103I ATGCAT 1 cut(s) 900
MseI TTAA 6 cut(s) 372, 408, 498, 684, 831, 1107
MslI CAYNNNNRTG 4 cut(s) 741, 780, 1320, 1376
MspA1I CMGCKG 2 cut(s) 104, 290
MspCI CTTAAG 1 cut(s) 497
MspI CCGG 3 cut(s) 74, 182, 260
MspR9I CCNGG 5 cut(s) 125, 311, 475, 1038, 1071
Mva1269I GAATGC 1 cut(s) 1383
MvaI CCWGG 5 cut(s) 125, 311, 475, 1038, 1071
NdeII GATC 6 cut(s) 132, 178, 318, 514, 769, 786
NlaIII CATG 5 cut(s) 627, 668, 740, 983, 1004
NlaIV GGNNCC 1 cut(s) 180
NmeAIII GCCGAG 1 cut(s) 418
NmuCI GTSAC 4 cut(s) 1206, 1223, 1264, 1322
NruI TCGCGA 3 cut(s) 117, 303, 1282
NsiI ATGCAT 1 cut(s) 900
NspI RCATGY 1 cut(s) 740
PaeI GCATGC 1 cut(s) 740
PaeR7I CTCGAG 1 cut(s) 876
PagI TCATGA 1 cut(s) 979
PaqCI CACCTGC 2 cut(s) 90, 276
PcsI WCGNNNNNNNCGW 3 cut(s) 182, 1099, 1254
PctI GAATGC 1 cut(s) 1383
PfeI GAWTC 7 cut(s) 20, 70, 206, 256, 569, 675, 962
Pfl23II CGTACG 2 cut(s) 25, 211
PpuMI RGGWCCY 1 cut(s) 637
Psp5II RGGWCCY 1 cut(s) 637
Psp6I CCWGG 5 cut(s) 123, 309, 473, 1036, 1069
PspEI GGTNACC 1 cut(s) 882
PspGI CCWGG 5 cut(s) 123, 309, 473, 1036, 1069
PspLI CGTACG 2 cut(s) 25, 211
PspN4I GGNNCC 1 cut(s) 180
PspPI GGNCC 1 cut(s) 637
PspPPI RGGWCCY 1 cut(s) 637
PstI CTGCAG 2 cut(s) 87, 273
PsuI RGATCY 1 cut(s) 178
RruI TCGCGA 3 cut(s) 117, 303, 1282
RsaI GTAC 5 cut(s) 27, 147, 213, 333, 752
RsaNI GTAC 5 cut(s) 26, 146, 212, 332, 751
RseI CAYNNNNRTG 4 cut(s) 741, 780, 1320, 1376
SacII CCGCGG 2 cut(s) 105, 291
SaqAI TTAA 6 cut(s) 372, 408, 498, 684, 831, 1107
Sau3AI GATC 6 cut(s) 132, 178, 318, 514, 769, 786
Sau96I GGNCC 1 cut(s) 637
ScrFI CCNGG 5 cut(s) 125, 311, 475, 1038, 1071
SfaNI GCATC 3 cut(s) 451, 907, 994
SfcI CTRYAG 2 cut(s) 83, 269
Sfr274I CTCGAG 1 cut(s) 876
Sfr303I CCGCGG 2 cut(s) 105, 291
SgrBI CCGCGG 2 cut(s) 105, 291
SinI GGWCC 1 cut(s) 637
SlaI CTCGAG 1 cut(s) 876
SmiI ATTTAAAT 1 cut(s) 832
SmiMI CAYNNNNRTG 4 cut(s) 741, 780, 1320, 1376
SmlI CTYRAG 2 cut(s) 497, 876
SmoI CTYRAG 2 cut(s) 497, 876
SphI GCATGC 1 cut(s) 740
Sse9I AATT 7 cut(s) 401, 546, 560, 716, 806, 833, 915
SspMI CTAG 4 cut(s) 416, 483, 1254, 1387
StyD4I CCNGG 5 cut(s) 123, 309, 473, 1036, 1069
StyI CCWWGG 3 cut(s) 166, 352, 640
SwaI ATTTAAAT 1 cut(s) 832
TaaI ACNGT 8 cut(s) 30, 157, 216, 343, 596, 730, 952, 1264
TaqI TCGA 8 cut(s) 32, 131, 218, 317, 517, 588, 877, 1334
TasI AATT 7 cut(s) 401, 546, 560, 716, 806, 833, 915
TatI WGTACW 3 cut(s) 145, 331, 750
TauI GCSGC 6 cut(s) 104, 107, 110, 290, 293, 296
TfiI GAWTC 7 cut(s) 20, 70, 206, 256, 569, 675, 962
Tru1I TTAA 6 cut(s) 372, 408, 498, 684, 831, 1107
Tru9I TTAA 6 cut(s) 372, 408, 498, 684, 831, 1107
TscAI CASTG 4 cut(s) 167, 353, 733, 1209
TseFI GTSAC 4 cut(s) 1206, 1223, 1264, 1322
TseI GCWGC 5 cut(s) 98, 284, 419, 470, 869
Tsp45I GTSAC 4 cut(s) 1206, 1223, 1264, 1322
TspDTI ATGAA 1 cut(s) 1017
TspGWI ACGGA 3 cut(s) 13, 199, 473
TspRI CASTG 4 cut(s) 167, 353, 733, 1209
Vha464I CTTAAG 1 cut(s) 497
VpaK11BI GGWCC 1 cut(s) 637
XagI CCTNNNNNAGG 2 cut(s) 86, 272
XapI RAATTY 2 cut(s) 716, 833
XceI RCATGY 1 cut(s) 740
XhoI CTCGAG 1 cut(s) 876
XspI CTAG 4 cut(s) 416, 483, 1254, 1387
Zsp2I ATGCAT 1 cut(s) 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.