Rmu_sc0002187.1_g000065

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002187.1
OG0015749
Physical Location & Seq
Forward (+)
272518 .. 278785
6268 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002187.1_g000065.1.cds

Sequence Viewer

Length: 1224 bp
atggcacattcaaaggctgtctcaaccccaactaagaatactacaaagtcactcaaagtaaggaagtcaaagtcgtcaaagtccaagaaggtatgtacatctgctgcaagcgtgagtaaaggaaagaatcgtggaaagaagatagaggaggatgaaggaagcactggaggattgaagctgctcaagcgtcaccctgttacaatgtcaaagatgaccaagggtagggttctaaagaagaggcttagagtccagtttgataggaatggctctccggtggggaaaaatgcagaaaagatgcaatcctacattggtgttttggcaagaacaaagatcccaatttcaattgcatcctggcttggtgttgacaagaaaaagaaaaacacgctttgggagactgttttggaaagacctgaacacaggaaaatggtgttacaatctgctggagccaaatggagggaatttaagagcagactaaccaccgtctatgtgttgccatatgtggatgatccggagatgttacaatttccaccagatgactataggtttattactgttgatgagtggactgcttttgtaacagaaagaacaaagcctgcatttttgggtattccaccacagccaaacgacaaagagtgcggctattttgtaatgagatacatgagggatatcatccatgatacaactttgtcatttgcagataagtggggtaggagagccaaccatacttatggtcaagtggaacttgatgaagtgcgtaatgagatttcaaattatgtgatgaagaacctattgaagctatgtagtgttggtcttttttggcttgtcgacagcatgtcggcagatctgccggtccatcagacgacgaattcagctgaaaaccgtcgacccaatgatcgcttacgaatgtggtcaccacatatctttatgggatctagatacggaatatacatgaaggttcctgatggacttcatttacccaagtcaagtggctctagatcacagagtgcgtctgcaatgaggttgaaacgcttactaagtgactacttgattgggaatggatatgtcagtgatgaattatgcttatgcatttccgtgacaagtttcggatttattatcgtcacggatcgacttcgtcccagggatgatgacaaagacatgttagaggtagaagtgccctacctaagtgcaataggcgcattactgtatttcgcgcaatgcacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

407

Amino Acids

46.29

Weight (kDa)

9.75

Isoelectric Point (pI)

36.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 825, 883
AccII CGCG 1 cut(s) 1211
AccIII TCCGGA 1 cut(s) 508
AciI CCGC 1 cut(s) 636
AclWI GGATC 4 cut(s) 325, 500, 937, 1131
AcsI RAATTY 2 cut(s) 458, 865
AdeI CACNNNGTG 1 cut(s) 1004
AfaI GTAC 1 cut(s) 97
AfiI CCNNNNNNNGG 2 cut(s) 222, 453
AflIII ACRYGT 1 cut(s) 1155
AgsI TTSAA 6 cut(s) 12, 175, 342, 768, 793, 1024
AhdI GACNNNNNGTC 1 cut(s) 832
AjnI CCWGG 2 cut(s) 350, 1136
AluBI AGCT 3 cut(s) 178, 796, 872
AluI AGCT 3 cut(s) 178, 796, 872
Alw26I GTCTC 2 cut(s) 25, 386
AlwI GGATC 4 cut(s) 325, 500, 937, 1131
Aor13HI TCCGGA 1 cut(s) 508
ApeKI GCWGC 2 cut(s) 104, 178
ApoI RAATTY 2 cut(s) 458, 865
AspLEI GCGC 2 cut(s) 1196, 1213
AspS9I GGNCC 1 cut(s) 850
AsuHPI GGTGA 2 cut(s) 182, 903
AvaII GGWCC 1 cut(s) 850
BaeGI GKGCMC 1 cut(s) 1176
BbvI GCAGC 2 cut(s) 91, 165
BccI CCATC 2 cut(s) 861, 956
BciT130I CCWGG 2 cut(s) 352, 1138
BcoDI GTCTC 2 cut(s) 25, 386
BfaI CTAG 2 cut(s) 933, 993
BfmI CTRYAG 1 cut(s) 538
BglII AGATCT 1 cut(s) 841
BisI GCNGC 3 cut(s) 105, 179, 637
BlsI GCNGC 3 cut(s) 106, 180, 638
Bme1390I CCNGG 2 cut(s) 352, 1138
Bme18I GGWCC 1 cut(s) 850
BmeRI GACNNNNNGTC 1 cut(s) 832
BmgT120I GGNCC 1 cut(s) 850
BmiI GGNNCC 2 cut(s) 445, 957
BmrFI CCNGG 2 cut(s) 352, 1138
BmsI GCATC 2 cut(s) 285, 356
BpmI CTGGAG 2 cut(s) 186, 462
BpuEI CTTGAG 1 cut(s) 167
BsaJI CCNNGG 3 cut(s) 216, 1136, 1137
BsaWI WCCGGW 2 cut(s) 271, 508
Bsc4I CCNNNNNNNGG 2 cut(s) 222, 453
Bse118I RCCGGY 1 cut(s) 847
Bse1I ACTGG 2 cut(s) 169, 250
Bse3DI GCAATG 2 cut(s) 1020, 1220
BseAI TCCGGA 1 cut(s) 508
BseBI CCWGG 2 cut(s) 352, 1138
BseDI CCNNGG 3 cut(s) 216, 1136, 1137
BseGI GGATG 5 cut(s) 157, 347, 508, 669, 1147
BseLI CCNNNNNNNGG 2 cut(s) 222, 453
BseMI GCAATG 2 cut(s) 1020, 1220
BseNI ACTGG 2 cut(s) 169, 250
BseRI GAGGAG 1 cut(s) 161
BseSI GKGCMC 1 cut(s) 1176
BseXI GCAGC 2 cut(s) 91, 165
Bsh1236I CGCG 1 cut(s) 1211
BsiSI CCGG 3 cut(s) 272, 509, 848
BslFI GGGAC 1 cut(s) 1119
BslI CCNNNNNNNGG 2 cut(s) 222, 453
BsmAI GTCTC 2 cut(s) 25, 386
BsmFI GGGAC 1 cut(s) 1119
Bsp1286I GDGCHC 1 cut(s) 1176
Bsp13I TCCGGA 1 cut(s) 508
Bsp1407I TGTACA 1 cut(s) 95
Bsp143I GATC 7 cut(s) 330, 505, 841, 892, 929, 995, 1123
BspACI CCGC 1 cut(s) 636
BspEI TCCGGA 1 cut(s) 508
BspFNI CGCG 1 cut(s) 1211
BspLI GGNNCC 2 cut(s) 445, 957
BspPI GGATC 4 cut(s) 325, 500, 937, 1131
BsrDI GCAATG 2 cut(s) 1020, 1220
BsrFI RCCGGY 1 cut(s) 847
BsrGI TGTACA 1 cut(s) 95
BsrI ACTGG 2 cut(s) 169, 250
BssAI RCCGGY 1 cut(s) 847
BssECI CCNNGG 3 cut(s) 216, 1136, 1137
BssMI GATC 7 cut(s) 330, 505, 841, 892, 929, 995, 1123
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 2 cut(s) 352, 1138
Bst4CI ACNGT 5 cut(s) 397, 481, 553, 881, 1203
Bst6I CTCTTC 1 cut(s) 230
BstAUI TGTACA 1 cut(s) 95
BstC8I GCNNGC 2 cut(s) 109, 594
BstDEI CTNAG 4 cut(s) 33, 242, 1034, 1181
BstEII GGTNACC 1 cut(s) 909
BstF5I GGATG 5 cut(s) 157, 347, 508, 669, 1147
BstFNI CGCG 1 cut(s) 1211
BstHHI GCGC 2 cut(s) 1196, 1213
BstKTI GATC 7 cut(s) 333, 508, 844, 895, 932, 998, 1126
BstMAI GTCTC 2 cut(s) 25, 386
BstMBI GATC 7 cut(s) 330, 505, 841, 892, 929, 995, 1123
BstMWI GCNNNNNNNGC 2 cut(s) 184, 1193
BstNI CCWGG 2 cut(s) 352, 1138
BstNSI RCATGY 2 cut(s) 835, 1159
BstPI GGTNACC 1 cut(s) 909
BstSCI CCNGG 2 cut(s) 350, 1136
BstSFI CTRYAG 1 cut(s) 538
BstSLI GKGCMC 1 cut(s) 1176
BstUI CGCG 1 cut(s) 1211
BstV1I GCAGC 2 cut(s) 91, 165
BstX2I RGATCY 3 cut(s) 330, 841, 929
BstXI CCANNNNNNTGG 1 cut(s) 728
BstYI RGATCY 3 cut(s) 330, 841, 929
BtsCI GGATG 5 cut(s) 157, 347, 508, 669, 1147
BtsIMutI CAGTG 2 cut(s) 162, 1072
Cac8I GCNNGC 2 cut(s) 109, 594
CfoI GCGC 2 cut(s) 1196, 1213
Cfr10I RCCGGY 1 cut(s) 847
Cfr13I GGNCC 1 cut(s) 850
CseI GACGC 2 cut(s) 176, 996
Csp6I GTAC 1 cut(s) 96
CspCI CAANNNNNGTGG 2 cut(s) 967, 1002
CviAII CATG 6 cut(s) 658, 674, 832, 949, 1156, 1221
CviQI GTAC 1 cut(s) 96
DdeI CTNAG 4 cut(s) 33, 242, 1034, 1181
DpnI GATC 7 cut(s) 332, 507, 843, 894, 931, 997, 1125
DpnII GATC 7 cut(s) 330, 505, 841, 892, 929, 995, 1123
DraIII CACNNNGTG 1 cut(s) 1004
DriI GACNNNNNGTC 1 cut(s) 832
Eam1104I CTCTTC 1 cut(s) 230
Eam1105I GACNNNNNGTC 1 cut(s) 832
EarI CTCTTC 1 cut(s) 230
Eco130I CCWWGG 1 cut(s) 216
Eco32I GATATC 1 cut(s) 667
Eco47I GGWCC 1 cut(s) 850
Eco91I GGTNACC 1 cut(s) 909
EcoO65I GGTNACC 1 cut(s) 909
EcoRI GAATTC 1 cut(s) 865
EcoRII CCWGG 2 cut(s) 350, 1136
EcoRV GATATC 1 cut(s) 667
EcoT14I CCWWGG 1 cut(s) 216
EcoT22I ATGCAT 1 cut(s) 1088
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 6 cut(s) 661, 677, 835, 952, 1159, 1224
FalI AAGNNNNNCTT 2 cut(s) 726, 758
FaqI GGGAC 1 cut(s) 1119
FatI CATG 6 cut(s) 657, 673, 831, 948, 1155, 1220
FauNDI CATATG 1 cut(s) 496
FblI GTMKAC 2 cut(s) 825, 883
Fnu4HI GCNGC 3 cut(s) 105, 179, 637
FokI GGATG 5 cut(s) 164, 334, 515, 656, 1154
Fsp4HI GCNGC 3 cut(s) 105, 179, 637
FspBI CTAG 2 cut(s) 933, 993
GlaI GCGC 2 cut(s) 1195, 1212
GluI GCNGC 3 cut(s) 105, 179, 637
GsuI CTGGAG 2 cut(s) 186, 462
HapII CCGG 3 cut(s) 272, 509, 848
HgaI GACGC 2 cut(s) 176, 996
HhaI GCGC 2 cut(s) 1196, 1213
Hin1II CATG 6 cut(s) 661, 677, 835, 952, 1159, 1224
Hin6I GCGC 2 cut(s) 1194, 1211
HinP1I GCGC 2 cut(s) 1194, 1211
HincII GTYRAC 3 cut(s) 364, 826, 884
HindII GTYRAC 3 cut(s) 364, 826, 884
HinfI GANTC 2 cut(s) 127, 246
HpaII CCGG 3 cut(s) 272, 509, 848
HphI GGTGA 2 cut(s) 182, 903
Hpy166II GTNNAC 4 cut(s) 364, 564, 826, 884
Hpy188I TCNGA 2 cut(s) 858, 1106
Hpy188III TCNNGA 4 cut(s) 509, 933, 959, 993
Hpy8I GTNNAC 4 cut(s) 364, 564, 826, 884
Hpy99I CGWCG 2 cut(s) 865, 885
HpyAV CCTTC 3 cut(s) 82, 149, 946
HpyCH4III ACNGT 5 cut(s) 397, 481, 553, 881, 1203
HpyF10VI GCNNNNNNNGC 2 cut(s) 184, 1193
HpyF3I CTNAG 4 cut(s) 33, 242, 1034, 1181
Hsp92II CATG 6 cut(s) 661, 677, 835, 952, 1159, 1224
HspAI GCGC 2 cut(s) 1194, 1211
Kpn2I TCCGGA 1 cut(s) 508
Kzo9I GATC 7 cut(s) 330, 505, 841, 892, 929, 995, 1123
LmnI GCTCC 1 cut(s) 443
Lsp1109I GCAGC 2 cut(s) 91, 165
LweI GCATC 2 cut(s) 285, 356
MaeI CTAG 2 cut(s) 933, 993
MalI GATC 7 cut(s) 332, 507, 843, 894, 931, 997, 1125
MboI GATC 7 cut(s) 330, 505, 841, 892, 929, 995, 1123
MboII GAAGA 3 cut(s) 151, 247, 793
MfeI CAATTG 1 cut(s) 342
MflI RGATCY 3 cut(s) 330, 841, 929
MhlI GDGCHC 1 cut(s) 1176
MluCI AATT 7 cut(s) 336, 342, 458, 521, 769, 865, 1073
MlyI GAGTC 1 cut(s) 255
MnlI CCTC 8 cut(s) 139, 142, 161, 231, 447, 654, 1011, 1156
Mph1103I ATGCAT 1 cut(s) 1088
MroI TCCGGA 1 cut(s) 508
MseI TTAA 1 cut(s) 462
MslI CAYNNNNRTG 2 cut(s) 726, 1091
MspA1I CMGCKG 1 cut(s) 872
MspI CCGG 3 cut(s) 272, 509, 848
MspR9I CCNGG 2 cut(s) 352, 1138
MunI CAATTG 1 cut(s) 342
MvaI CCWGG 2 cut(s) 352, 1138
MvnI CGCG 1 cut(s) 1211
MwoI GCNNNNNNNGC 2 cut(s) 184, 1193
NdeI CATATG 1 cut(s) 496
NdeII GATC 7 cut(s) 330, 505, 841, 892, 929, 995, 1123
NlaIII CATG 6 cut(s) 661, 677, 835, 952, 1159, 1224
NlaIV GGNNCC 2 cut(s) 445, 957
NmuCI GTSAC 6 cut(s) 48, 188, 909, 1037, 1093, 1117
NsiI ATGCAT 1 cut(s) 1088
NspI RCATGY 2 cut(s) 835, 1159
PasI CCCWGGG 1 cut(s) 1137
PciI ACATGT 1 cut(s) 1155
PfeI GAWTC 1 cut(s) 127
PflFI GACNNNGTC 1 cut(s) 1131
PkrI GCNGC 3 cut(s) 106, 180, 638
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
PscI ACATGT 1 cut(s) 1155
Psp6I CCWGG 2 cut(s) 350, 1136
PspEI GGTNACC 1 cut(s) 909
PspGI CCWGG 2 cut(s) 350, 1136
PspN4I GGNNCC 2 cut(s) 445, 957
PspPI GGNCC 1 cut(s) 850
PsuI RGATCY 3 cut(s) 330, 841, 929
PsyI GACNNNGTC 1 cut(s) 1131
PvuII CAGCTG 1 cut(s) 872
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
RseI CAYNNNNRTG 2 cut(s) 726, 1091
SalI GTCGAC 2 cut(s) 824, 882
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 3 cut(s) 105, 179, 637
Sau3AI GATC 7 cut(s) 330, 505, 841, 892, 929, 995, 1123
Sau96I GGNCC 1 cut(s) 850
SchI GAGTC 1 cut(s) 255
ScrFI CCNGG 2 cut(s) 352, 1138
SduI GDGCHC 1 cut(s) 1176
SfaNI GCATC 2 cut(s) 285, 356
SfcI CTRYAG 1 cut(s) 538
SinI GGWCC 1 cut(s) 850
SmiMI CAYNNNNRTG 2 cut(s) 726, 1091
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
Sse9I AATT 7 cut(s) 336, 342, 458, 521, 769, 865, 1073
SsiI CCGC 1 cut(s) 636
SspMI CTAG 2 cut(s) 933, 993
StyD4I CCNGG 2 cut(s) 350, 1136
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 5 cut(s) 397, 481, 553, 881, 1203
TaqI TCGA 3 cut(s) 825, 883, 1126
TasI AATT 7 cut(s) 336, 342, 458, 521, 769, 865, 1073
TatI WGTACW 1 cut(s) 95
TauI GCSGC 1 cut(s) 639
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TscAI CASTG 2 cut(s) 169, 1072
TseFI GTSAC 6 cut(s) 48, 188, 909, 1037, 1093, 1117
TseI GCWGC 2 cut(s) 104, 178
Tsp45I GTSAC 6 cut(s) 48, 188, 909, 1037, 1093, 1117
TspDTI ATGAA 6 cut(s) 168, 762, 794, 959, 965, 1086
TspGWI ACGGA 3 cut(s) 954, 1081, 1136
TspRI CASTG 2 cut(s) 169, 1072
Tth111I GACNNNGTC 1 cut(s) 1131
VpaK11BI GGWCC 1 cut(s) 850
XapI RAATTY 2 cut(s) 458, 865
XbaI TCTAGA 2 cut(s) 932, 992
XceI RCATGY 2 cut(s) 835, 1159
XmiI GTMKAC 2 cut(s) 825, 883
XspI CTAG 2 cut(s) 933, 993
Zsp2I ATGCAT 1 cut(s) 1088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.