Rorug06G0055000

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004259
Physical Location & Seq
Reverse (-)
7550607 .. 7552813
2207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0055000.1

Sequence Viewer

Length: 705 bp
ATGGAAGAGAATGAAGACGGCGGCGTCGACAATGACTGCTACAAGGTGCGACCGTCCCTGACATATTCTGTGGACATCTGTAAGGATGTTCTCTGCAATGTGGATAAACTGAAATTACTGAATCTCGAACTTACTTCAGTGTTGCAACCGGGTTTTGCTGTTCACGGCCTTGGATGCAAATTGAAAGGGGAGCAACTGGAAGTTTCAATCGCTCTAAATACAAAGCCTCCGCAGTATTTTGTCTTCCGAAGGGCTGCGAATGCTCTTGTTGCTGTCTCAAATGGTTTCCCTGCAGACTTGGCATTGAAAGTACTGTCAGGCGATATGGAGATAGCCGCCCACCACATGAAACACAGTCGGGTTAATCAGGAAGTATTGCATAAACGGCACATCAATTTTATTCGCCTACATCGTGAGTATGAACTTGGTCGCAAGTTGCCCTGTGCTGTACTTAAACATTATGAACACTGTATTTTTCTTGTGGCAAATCCACTAGTACGTCGACTAATGGGCCAACTCATAATGCGATACATTTTTGGCGGCACTGATTTTGAACCCTGGGCCAATGTGGTGGTGCAATCTTATGAGCGGTTCAAGGATCGCCCATGTCCCTTCTTGAGTATGACCCCGCTTTACACGTGCGGAGATGATGCTACGATGGAAGCACCAAATGATGAAATGCGCAATCTTAATCTGAAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

234

Amino Acids

26.59

Weight (kDa)

7.1

Isoelectric Point (pI)

26.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 23
Acc16I TGCGCA 1 cut(s) 683
AccBSI CCGCTC 1 cut(s) 589
AccI GTMKAC 2 cut(s) 27, 502
AciI CCGC 7 cut(s) 21, 230, 336, 540, 589, 629, 642
AclWI GGATC 1 cut(s) 606
AcuI CTGAAG 1 cut(s) 120
AcvI CACGTG 1 cut(s) 639
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 3 cut(s) 312, 450, 498
AflIII ACRYGT 1 cut(s) 636
AgsI TTSAA 5 cut(s) 184, 207, 307, 554, 595
AhlI ACTAGT 1 cut(s) 493
AjnI CCWGG 1 cut(s) 557
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 1 cut(s) 606
AoxI GGCC 3 cut(s) 166, 511, 561
ApeKI GCWGC 1 cut(s) 254
AspLEI GCGC 1 cut(s) 684
AspS9I GGNCC 2 cut(s) 511, 561
AsuC2I CCSGG 1 cut(s) 150
BarI GAAGNNNNNNTAC 2 cut(s) 227, 259
BbrPI CACGTG 1 cut(s) 639
BbsI GAAGAC 2 cut(s) 21, 235
BbvI GCAGC 1 cut(s) 241
BccI CCATC 1 cut(s) 652
BceAI ACGGC 3 cut(s) 34, 181, 401
BciT130I CCWGG 1 cut(s) 559
BcnI CCSGG 1 cut(s) 150
BcoDI GTCTC 1 cut(s) 280
BcuI ACTAGT 1 cut(s) 493
BfaI CTAG 1 cut(s) 494
BfmI CTRYAG 1 cut(s) 291
BisI GCNGC 4 cut(s) 22, 255, 336, 541
BlsI GCNGC 4 cut(s) 23, 256, 337, 542
BmcAI AGTACT 1 cut(s) 312
Bme1390I CCNGG 2 cut(s) 150, 559
BmgT120I GGNCC 2 cut(s) 511, 561
BmrFI CCNGG 2 cut(s) 150, 559
BmsI GCATC 2 cut(s) 164, 640
BpiI GAAGAC 2 cut(s) 21, 235
BpuEI CTTGAG 1 cut(s) 637
BpuMI CCSGG 1 cut(s) 150
BsaAI YACGTR 1 cut(s) 639
BsaHI GRCGYC 1 cut(s) 24
BsaJI CCNNGG 3 cut(s) 169, 557, 558
BsaXI ACNNNNNCTCC 2 cut(s) 211, 241
Bse1I ACTGG 1 cut(s) 201
Bse3DI GCAATG 1 cut(s) 103
BseBI CCWGG 1 cut(s) 559
BseDI CCNNGG 3 cut(s) 169, 557, 558
BseGI GGATG 2 cut(s) 91, 179
BseMI GCAATG 1 cut(s) 103
BseNI ACTGG 1 cut(s) 201
BseXI GCAGC 1 cut(s) 241
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 3 cut(s) 168, 513, 563
BsiEI CGRYCG 1 cut(s) 53
BsiSI CCGG 1 cut(s) 149
BslFI GGGAC 2 cut(s) 40, 594
BsmAI GTCTC 1 cut(s) 280
BsmFI GGGAC 2 cut(s) 40, 594
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 3 cut(s) 168, 513, 563
Bsp143I GATC 1 cut(s) 598
BspACI CCGC 7 cut(s) 21, 230, 336, 540, 589, 629, 642
BspANI GGCC 3 cut(s) 168, 513, 563
BspMAI CTGCAG 1 cut(s) 295
BspPI GGATC 1 cut(s) 606
BsrBI CCGCTC 1 cut(s) 589
BsrDI GCAATG 1 cut(s) 103
BsrI ACTGG 1 cut(s) 201
BssECI CCNNGG 3 cut(s) 169, 557, 558
BssMI GATC 1 cut(s) 598
BssNI GRCGYC 1 cut(s) 24
BssT1I CCWWGG 1 cut(s) 169
Bst2UI CCWGG 1 cut(s) 559
Bst4CI ACNGT 4 cut(s) 54, 315, 356, 470
Bst6I CTCTTC 1 cut(s) 692
BstACI GRCGYC 1 cut(s) 24
BstBAI YACGTR 1 cut(s) 639
BstF5I GGATG 2 cut(s) 91, 179
BstHHI GCGC 1 cut(s) 684
BstKTI GATC 1 cut(s) 601
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 1 cut(s) 598
BstMCI CGRYCG 1 cut(s) 53
BstMWI GCNNNNNNNGC 5 cut(s) 174, 260, 269, 299, 385
BstNI CCWGG 1 cut(s) 559
BstSCI CCNGG 2 cut(s) 148, 557
BstSFI CTRYAG 1 cut(s) 291
BstV1I GCAGC 1 cut(s) 241
BstV2I GAAGAC 2 cut(s) 21, 235
BstXI CCANNNNNNTGG 1 cut(s) 571
BsuRI GGCC 3 cut(s) 168, 513, 563
BtsCI GGATG 2 cut(s) 91, 179
BtsIMutI CAGTG 3 cut(s) 144, 466, 543
CfoI GCGC 1 cut(s) 684
Cfr13I GGNCC 2 cut(s) 511, 561
CseI GACGC 1 cut(s) 13
Csp6I GTAC 3 cut(s) 311, 449, 497
CviAII CATG 2 cut(s) 346, 606
CviJI RGCY 6 cut(s) 168, 226, 254, 335, 513, 563
CviKI_1 RGCY 6 cut(s) 168, 226, 254, 335, 513, 563
CviQI GTAC 3 cut(s) 311, 449, 497
DpnI GATC 1 cut(s) 600
DpnII GATC 1 cut(s) 598
DrdI GACNNNNNNGTC 1 cut(s) 23
DseDI GACNNNNNNGTC 1 cut(s) 23
Eam1104I CTCTTC 1 cut(s) 692
EarI CTCTTC 1 cut(s) 692
Eco130I CCWWGG 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 120
Eco72I CACGTG 1 cut(s) 639
EcoRII CCWGG 1 cut(s) 557
EcoT14I CCWWGG 1 cut(s) 169
ErhI CCWWGG 1 cut(s) 169
FaeI CATG 2 cut(s) 349, 609
FaqI GGGAC 2 cut(s) 40, 594
FatI CATG 2 cut(s) 345, 605
FauI CCCGC 1 cut(s) 636
FblI GTMKAC 2 cut(s) 27, 502
Fnu4HI GCNGC 4 cut(s) 22, 255, 336, 541
FokI GGATG 2 cut(s) 98, 186
Fsp4HI GCNGC 4 cut(s) 22, 255, 336, 541
FspBI CTAG 1 cut(s) 494
FspI TGCGCA 1 cut(s) 683
GlaI GCGC 1 cut(s) 683
GluI GCNGC 4 cut(s) 22, 255, 336, 541
HaeIII GGCC 3 cut(s) 168, 513, 563
HapII CCGG 1 cut(s) 149
HgaI GACGC 1 cut(s) 13
HhaI GCGC 1 cut(s) 684
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 2 cut(s) 349, 609
Hin6I GCGC 1 cut(s) 682
HinP1I GCGC 1 cut(s) 682
HincII GTYRAC 2 cut(s) 28, 503
HindII GTYRAC 2 cut(s) 28, 503
HinfI GANTC 1 cut(s) 121
HpaII CCGG 1 cut(s) 149
Hpy166II GTNNAC 4 cut(s) 28, 73, 163, 503
Hpy188I TCNGA 2 cut(s) 248, 696
Hpy188III TCNNGA 4 cut(s) 125, 368, 413, 616
Hpy8I GTNNAC 4 cut(s) 28, 73, 163, 503
Hpy99I CGWCG 2 cut(s) 29, 504
HpyAV CCTTC 2 cut(s) 243, 622
HpyCH4III ACNGT 4 cut(s) 54, 315, 356, 470
HpyCH4IV ACGT 2 cut(s) 499, 638
HpyCH4V TGCA 6 cut(s) 96, 145, 177, 293, 379, 577
HpyF10VI GCNNNNNNNGC 5 cut(s) 174, 260, 269, 299, 385
HpySE526I ACGT 2 cut(s) 499, 638
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 2 cut(s) 349, 609
HspAI GCGC 1 cut(s) 682
Kzo9I GATC 1 cut(s) 598
LmnI GCTCC 1 cut(s) 190
LpnPI CCDG 9 cut(s) 71, 162, 182, 303, 303, 353, 454, 544, 571
Lsp1109I GCAGC 1 cut(s) 241
LweI GCATC 2 cut(s) 164, 640
MaeI CTAG 1 cut(s) 494
MaeII ACGT 2 cut(s) 499, 638
MalI GATC 1 cut(s) 600
MbiI CCGCTC 1 cut(s) 589
MboI GATC 1 cut(s) 598
MboII GAAGA 3 cut(s) 17, 26, 235
MluCI AATT 3 cut(s) 113, 179, 394
MnlI CCTC 1 cut(s) 237
MseI TTAA 3 cut(s) 363, 453, 690
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 2 cut(s) 150, 559
Mva1269I GAATGC 1 cut(s) 265
MvaI CCWGG 1 cut(s) 559
MwoI GCNNNNNNNGC 5 cut(s) 174, 260, 269, 299, 385
NciI CCSGG 1 cut(s) 150
NdeII GATC 1 cut(s) 598
NlaIII CATG 2 cut(s) 349, 609
NsbI TGCGCA 1 cut(s) 683
PasI CCCWGGG 1 cut(s) 558
PcsI WCGNNNNNNNCGW 2 cut(s) 24, 409
PctI GAATGC 1 cut(s) 265
PfeI GAWTC 1 cut(s) 121
PkrI GCNGC 4 cut(s) 23, 256, 337, 542
PmaCI CACGTG 1 cut(s) 639
PmlI CACGTG 1 cut(s) 639
Ppu21I YACGTR 1 cut(s) 639
Psp6I CCWGG 1 cut(s) 557
PspCI CACGTG 1 cut(s) 639
PspGI CCWGG 1 cut(s) 557
PspPI GGNCC 2 cut(s) 511, 561
PstI CTGCAG 1 cut(s) 295
RsaI GTAC 3 cut(s) 312, 450, 498
RsaNI GTAC 3 cut(s) 311, 449, 497
SalI GTCGAC 2 cut(s) 26, 501
SaqAI TTAA 3 cut(s) 363, 453, 690
SatI GCNGC 4 cut(s) 22, 255, 336, 541
Sau3AI GATC 1 cut(s) 598
Sau96I GGNCC 2 cut(s) 511, 561
ScaI AGTACT 1 cut(s) 312
ScrFI CCNGG 2 cut(s) 150, 559
SetI ASST 3 cut(s) 48, 502, 641
SfaNI GCATC 2 cut(s) 164, 640
SfcI CTRYAG 1 cut(s) 291
SmlI CTYRAG 1 cut(s) 616
SmoI CTYRAG 1 cut(s) 616
SpeI ACTAGT 1 cut(s) 493
Sse9I AATT 3 cut(s) 113, 179, 394
SsiI CCGC 7 cut(s) 21, 230, 336, 540, 589, 629, 642
SspMI CTAG 1 cut(s) 494
StyD4I CCNGG 2 cut(s) 148, 557
StyI CCWWGG 1 cut(s) 169
TaaI ACNGT 4 cut(s) 54, 315, 356, 470
TaiI ACGT 2 cut(s) 502, 641
TaqI TCGA 3 cut(s) 27, 126, 502
TasI AATT 3 cut(s) 113, 179, 394
TatI WGTACW 2 cut(s) 310, 448
TauI GCSGC 3 cut(s) 24, 338, 543
TfiI GAWTC 1 cut(s) 121
Tru1I TTAA 3 cut(s) 363, 453, 690
Tru9I TTAA 3 cut(s) 363, 453, 690
TscAI CASTG 3 cut(s) 144, 473, 550
TseI GCWGC 1 cut(s) 254
TspDTI ATGAA 5 cut(s) 27, 362, 435, 477, 690
TspRI CASTG 3 cut(s) 144, 473, 550
XmiI GTMKAC 2 cut(s) 27, 502
XspI CTAG 1 cut(s) 494
ZrmI AGTACT 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.