Rh2AG353900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0004259
Physical Location & Seq
Reverse (-)
52283148 .. 52283637
490 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG353900.1

Sequence Viewer

Length: 432 bp
ATGAACATGAGGAATCCCCTTCCAGTTGGCAACTCTGGAACTCCCATGCAGTATCAGCCACCCGAGGTCCCTCCGGCTCCTCCGACCTCACCGACTGGTGCGGTGGAAGTCCATGCTCATCCTCCTTTTACCATTCATGCAGACGATCAGACAATTCCATTTCGAAATCGCAACGTCGAAGAAGAACCGTCTCGTGTTTGTATCGATAACTGCAGGAGGCTGTTGCCTATGGTGGATATTTTGACAATACTGTCTCCCCAAGTTGACATATGGAGTATGCTTTCTTGGATCAATATGGCACCTTCCTTGATAGTAATCATGAGACTGATTACTTTCGCCTGCAAGACTCCTTTGTTCACGATGACTCCTTTCGTCTTGCAGTCATCAACCGCCTTGTCCATTACCACTTCAACAGCGGTATCAATGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

143

Amino Acids

15.8

Weight (kDa)

5.82

Isoelectric Point (pI)

58.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 250
AccB1I GGYRCC 1 cut(s) 298
AciI CCGC 3 cut(s) 101, 390, 416
AclWI GGATC 1 cut(s) 296
AgsI TTSAA 1 cut(s) 411
Alw26I GTCTC 3 cut(s) 195, 258, 316
AlwI GGATC 1 cut(s) 296
Ama87I CYCGRG 1 cut(s) 62
AspS9I GGNCC 1 cut(s) 67
AsuHPI GGTGA 1 cut(s) 81
AsuII TTCGAA 1 cut(s) 163
AvaI CYCGRG 1 cut(s) 62
AvaII GGWCC 1 cut(s) 67
BaeI ACNNNNGTAYC 1 cut(s) 402
BanI GGYRCC 1 cut(s) 298
BauI CACGAG 1 cut(s) 192
BcoDI GTCTC 3 cut(s) 195, 258, 316
BfmI CTRYAG 1 cut(s) 211
Bme18I GGWCC 1 cut(s) 67
BmeT110I CYCGRG 1 cut(s) 62
BmgT120I GGNCC 1 cut(s) 67
BmiI GGNNCC 3 cut(s) 69, 78, 300
Bpu14I TTCGAA 1 cut(s) 163
Bsa29I ATCGAT 1 cut(s) 204
BsaBI GATNNNNATC 1 cut(s) 314
BsaJI CCNNGG 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 349, 379
Bse1I ACTGG 2 cut(s) 23, 100
Bse8I GATNNNNATC 1 cut(s) 314
BseCI ATCGAT 1 cut(s) 204
BseDI CCNNGG 1 cut(s) 63
BseGI GGATG 1 cut(s) 118
BseJI GATNNNNATC 1 cut(s) 314
BseNI ACTGG 2 cut(s) 23, 100
BseRI GAGGAG 1 cut(s) 69
BshNI GGYRCC 1 cut(s) 298
BshVI ATCGAT 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 62
BsiSI CCGG 1 cut(s) 74
BslFI GGGAC 1 cut(s) 53
BsmAI GTCTC 3 cut(s) 195, 258, 316
BsmBI CGTCTC 1 cut(s) 195
BsmFI GGGAC 1 cut(s) 53
BsoBI CYCGRG 1 cut(s) 62
Bsp119I TTCGAA 1 cut(s) 163
Bsp143I GATC 2 cut(s) 145, 288
BspACI CCGC 3 cut(s) 101, 390, 416
BspDI ATCGAT 1 cut(s) 204
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 3 cut(s) 69, 78, 300
BspMAI CTGCAG 1 cut(s) 215
BspPI GGATC 1 cut(s) 296
BspT104I TTCGAA 1 cut(s) 163
BspT107I GGYRCC 1 cut(s) 298
BsrI ACTGG 2 cut(s) 23, 100
BssECI CCNNGG 1 cut(s) 63
BssMI GATC 2 cut(s) 145, 288
BssSI CACGAG 1 cut(s) 192
Bst2BI CACGAG 1 cut(s) 192
Bst4CI ACNGT 2 cut(s) 189, 252
BstBI TTCGAA 1 cut(s) 163
BstC8I GCNNGC 1 cut(s) 340
BstF5I GGATG 1 cut(s) 118
BstKTI GATC 2 cut(s) 148, 291
BstMAI GTCTC 3 cut(s) 195, 258, 316
BstMBI GATC 2 cut(s) 145, 288
BstMWI GCNNNNNNNGC 1 cut(s) 55
BstSFI CTRYAG 1 cut(s) 211
Bsu15I ATCGAT 1 cut(s) 204
BsuTUI ATCGAT 1 cut(s) 204
BtsCI GGATG 1 cut(s) 118
Cac8I GCNNGC 1 cut(s) 340
CciI TCATGA 1 cut(s) 318
Cfr13I GGNCC 1 cut(s) 67
ClaI ATCGAT 1 cut(s) 204
CviAII CATG 5 cut(s) 7, 46, 113, 137, 319
CviJI RGCY 3 cut(s) 58, 77, 220
CviKI_1 RGCY 3 cut(s) 58, 77, 220
DpnI GATC 2 cut(s) 147, 290
DpnII GATC 2 cut(s) 145, 288
DrdI GACNNNNNNGTC 1 cut(s) 250
DseDI GACNNNNNNGTC 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 67
Eco88I CYCGRG 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 67
Esp3I CGTCTC 1 cut(s) 195
FaeI CATG 5 cut(s) 10, 49, 116, 140, 322
FaqI GGGAC 1 cut(s) 53
FatI CATG 5 cut(s) 6, 45, 112, 136, 318
FauNDI CATATG 1 cut(s) 269
FokI GGATG 1 cut(s) 105
HapII CCGG 1 cut(s) 74
Hin1II CATG 5 cut(s) 10, 49, 116, 140, 322
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 3 cut(s) 13, 346, 364
HpaII CCGG 1 cut(s) 74
HphI GGTGA 1 cut(s) 81
Hpy166II GTNNAC 2 cut(s) 265, 357
Hpy188I TCNGA 2 cut(s) 84, 150
Hpy188III TCNNGA 3 cut(s) 36, 319, 358
Hpy8I GTNNAC 2 cut(s) 265, 357
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 2 cut(s) 29, 312
HpyCH4III ACNGT 2 cut(s) 189, 252
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 5 cut(s) 49, 140, 213, 342, 379
HpyF10VI GCNNNNNNNGC 1 cut(s) 55
HpySE526I ACGT 1 cut(s) 174
Hsp92II CATG 5 cut(s) 10, 49, 116, 140, 322
Kzo9I GATC 2 cut(s) 145, 288
LmnI GCTCC 1 cut(s) 82
LpnPI CCDG 6 cut(s) 21, 36, 81, 87, 199, 352
MaeII ACGT 1 cut(s) 174
MalI GATC 2 cut(s) 147, 290
MboI GATC 2 cut(s) 145, 288
MboII GAAGA 2 cut(s) 191, 194
MluCI AATT 1 cut(s) 153
MlyI GAGTC 2 cut(s) 340, 358
MmeI TCCRAC 1 cut(s) 107
MnlI CCTC 7 cut(s) 3, 58, 81, 90, 97, 132, 210
MspA1I CMGCKG 1 cut(s) 416
MspI CCGG 1 cut(s) 74
MwoI GCNNNNNNNGC 1 cut(s) 55
NdeI CATATG 1 cut(s) 269
NdeII GATC 2 cut(s) 145, 288
NlaIII CATG 5 cut(s) 10, 49, 116, 140, 322
NlaIV GGNNCC 3 cut(s) 69, 78, 300
NspV TTCGAA 1 cut(s) 163
PagI TCATGA 1 cut(s) 318
PfeI GAWTC 1 cut(s) 13
PleI GAGTC 2 cut(s) 340, 358
PpsI GAGTC 2 cut(s) 340, 358
PpuMI RGGWCCY 1 cut(s) 67
Psp5II RGGWCCY 1 cut(s) 67
PspN4I GGNNCC 3 cut(s) 69, 78, 300
PspPI GGNCC 1 cut(s) 67
PspPPI RGGWCCY 1 cut(s) 67
PstI CTGCAG 1 cut(s) 215
Sau3AI GATC 2 cut(s) 145, 288
Sau96I GGNCC 1 cut(s) 67
SchI GAGTC 2 cut(s) 340, 358
SetI ASST 5 cut(s) 69, 89, 177, 304, 431
SfcI CTRYAG 1 cut(s) 211
SfuI TTCGAA 1 cut(s) 163
SinI GGWCC 1 cut(s) 67
Sse9I AATT 1 cut(s) 153
SsiI CCGC 3 cut(s) 101, 390, 416
TaaI ACNGT 2 cut(s) 189, 252
TaiI ACGT 1 cut(s) 177
TaqI TCGA 3 cut(s) 163, 177, 204
TasI AATT 1 cut(s) 153
TfiI GAWTC 1 cut(s) 13
TspDTI ATGAA 2 cut(s) 17, 125
VpaK11BI GGWCC 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.