Rh4BG182500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004259
Physical Location & Seq
Reverse (-)
32095720 .. 32096637
918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG182500.1

Sequence Viewer

Length: 399 bp
ATGTCGCGCACCGACGACAACTGCCCTCTTCAGAAATGGTGTCACTTGTGCGGTCAGATTCAGCCTTATGCGAGGGGACCTAAGGCCGAAGAATGCCCACCTCGTGTAGGTGAACACCCTCGTCAAGCACCACCAGATGAGAGTTCGAGATGCCCTTTATGTGAGGGCAACACGAGTATCATGGCACATTGGGTGCACCAAATAAAGAGCAAGCGGCCCTATTGGCAGTGTTGTCGAACTAATGGGTACCCTTATTGCAGGGGTTATCGGTGGGTAGATGAGGTGGCGGAAGGATCCACAGGGCCGACGGTCCACAGACCATCATTCATTTGGGGAGATGAGTGCAATATCCAGTTTGCGGGTTTCAACGGGTTCACTGACTATGAAGAACCTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

15.17

Weight (kDa)

5.93

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 246
AccB1I GGYRCC 1 cut(s) 246
AccII CGCG 1 cut(s) 7
AciI CCGC 4 cut(s) 51, 214, 287, 359
AclWI GGATC 2 cut(s) 288, 301
AcuI CTGAAG 1 cut(s) 14
AdeI CACNNNGTG 1 cut(s) 104
AfaI GTAC 1 cut(s) 248
AfiI CCNNNNNNNGG 2 cut(s) 107, 358
AgsI TTSAA 1 cut(s) 367
Alw21I GWGCWC 1 cut(s) 198
Alw44I GTGCAC 1 cut(s) 194
AlwI GGATC 2 cut(s) 288, 301
AoxI GGCC 3 cut(s) 84, 215, 302
ApaLI GTGCAC 1 cut(s) 194
Asp718I GGTACC 1 cut(s) 246
AspLEI GCGC 1 cut(s) 9
AspS9I GGNCC 4 cut(s) 77, 216, 302, 310
AsuHPI GGTGA 1 cut(s) 122
AvaII GGWCC 2 cut(s) 77, 310
AxyI CCTNAGG 1 cut(s) 81
BaeGI GKGCMC 1 cut(s) 198
BaeI ACNNNNGTAYC 2 cut(s) 160, 193
BamHI GGATCC 1 cut(s) 293
BanI GGYRCC 1 cut(s) 246
BauI CACGAG 2 cut(s) 102, 172
Bbv12I GWGCWC 1 cut(s) 198
BccI CCATC 1 cut(s) 328
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BglI GCCNNNNNGGC 1 cut(s) 223
BisI GCNGC 1 cut(s) 215
BlsI GCNGC 1 cut(s) 216
Bme18I GGWCC 2 cut(s) 77, 310
BmgT120I GGNCC 4 cut(s) 77, 216, 302, 310
BmiI GGNNCC 3 cut(s) 78, 248, 295
BmsI GCATC 1 cut(s) 140
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 358
Bse1I ACTGG 1 cut(s) 352
Bse21I CCTNAGG 1 cut(s) 81
BseLI CCNNNNNNNGG 2 cut(s) 107, 358
BseNI ACTGG 1 cut(s) 352
BseSI GKGCMC 1 cut(s) 198
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 3 cut(s) 86, 217, 304
BshNI GGYRCC 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 198
BslFI GGGAC 1 cut(s) 90
BslI CCNNNNNNNGG 2 cut(s) 107, 358
BsmFI GGGAC 1 cut(s) 90
BsmI GAATGC 1 cut(s) 98
BsnI GGCC 3 cut(s) 86, 217, 304
Bsp1286I GDGCHC 1 cut(s) 198
Bsp143I GATC 1 cut(s) 293
BspACI CCGC 4 cut(s) 51, 214, 287, 359
BspANI GGCC 3 cut(s) 86, 217, 304
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 3 cut(s) 78, 248, 295
BspPI GGATC 2 cut(s) 288, 301
BspT107I GGYRCC 1 cut(s) 246
BsrI ACTGG 1 cut(s) 352
BssMI GATC 1 cut(s) 293
BssSI CACGAG 2 cut(s) 102, 172
Bst2BI CACGAG 2 cut(s) 102, 172
Bst4CI ACNGT 1 cut(s) 310
Bst6I CTCTTC 1 cut(s) 33
BstC8I GCNNGC 1 cut(s) 212
BstDEI CTNAG 1 cut(s) 81
BstENI CCTNNNNNAGG 1 cut(s) 105
BstFNI CGCG 1 cut(s) 7
BstHHI GCGC 1 cut(s) 9
BstKTI GATC 1 cut(s) 296
BstMBI GATC 1 cut(s) 293
BstMWI GCNNNNNNNGC 1 cut(s) 223
BstSLI GKGCMC 1 cut(s) 198
BstUI CGCG 1 cut(s) 7
BstX2I RGATCY 1 cut(s) 293
BstYI RGATCY 1 cut(s) 293
Bsu36I CCTNAGG 1 cut(s) 81
BsuRI GGCC 3 cut(s) 86, 217, 304
BtsI GCAGTG 1 cut(s) 233
BtsIMutI CAGTG 2 cut(s) 233, 375
Cac8I GCNNGC 1 cut(s) 212
CfoI GCGC 1 cut(s) 9
Cfr13I GGNCC 4 cut(s) 77, 216, 302, 310
Csp6I GTAC 1 cut(s) 247
CviAII CATG 1 cut(s) 181
CviJI RGCY 4 cut(s) 64, 86, 217, 304
CviKI_1 RGCY 4 cut(s) 64, 86, 217, 304
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 1 cut(s) 81
DpnI GATC 1 cut(s) 295
DpnII GATC 1 cut(s) 293
DraIII CACNNNGTG 1 cut(s) 104
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
EciI GGCGGA 1 cut(s) 302
Eco47I GGWCC 2 cut(s) 77, 310
Eco57I CTGAAG 1 cut(s) 14
Eco81I CCTNAGG 1 cut(s) 81
EcoNI CCTNNNNNAGG 1 cut(s) 105
EcoO109I RGGNCCY 1 cut(s) 77
FaeI CATG 1 cut(s) 184
FaiI YATR 4 cut(s) 69, 160, 182, 384
FaqI GGGAC 1 cut(s) 90
FatI CATG 1 cut(s) 180
FauI CCCGC 1 cut(s) 352
Fnu4HI GCNGC 1 cut(s) 215
Fsp4HI GCNGC 1 cut(s) 215
GlaI GCGC 1 cut(s) 8
GluI GCNGC 1 cut(s) 215
HaeIII GGCC 3 cut(s) 86, 217, 304
HhaI GCGC 1 cut(s) 9
Hin1II CATG 1 cut(s) 184
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HinfI GANTC 1 cut(s) 58
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 4 cut(s) 113, 196, 313, 375
Hpy188I TCNGA 2 cut(s) 33, 57
Hpy188III TCNNGA 1 cut(s) 147
Hpy8I GTNNAC 4 cut(s) 113, 196, 313, 375
Hpy99I CGWCG 2 cut(s) 17, 310
HpyAV CCTTC 1 cut(s) 284
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4V TGCA 3 cut(s) 196, 258, 345
HpyF10VI GCNNNNNNNGC 1 cut(s) 223
HpyF3I CTNAG 1 cut(s) 81
Hsp92II CATG 1 cut(s) 184
HspAI GCGC 1 cut(s) 7
KpnI GGTACC 1 cut(s) 250
Kzo9I GATC 1 cut(s) 293
LpnPI CCDG 4 cut(s) 147, 244, 285, 365
LweI GCATC 1 cut(s) 140
MaeIII GTNAC 1 cut(s) 41
MalI GATC 1 cut(s) 295
MboI GATC 1 cut(s) 293
MboII GAAGA 3 cut(s) 20, 101, 398
MflI RGATCY 1 cut(s) 293
MhlI GDGCHC 1 cut(s) 198
MnlI CCTC 6 cut(s) 36, 66, 111, 129, 157, 274
Mva1269I GAATGC 1 cut(s) 98
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 1 cut(s) 223
NdeII GATC 1 cut(s) 293
NlaIII CATG 1 cut(s) 184
NlaIV GGNNCC 3 cut(s) 78, 248, 295
NmuCI GTSAC 1 cut(s) 41
PctI GAATGC 1 cut(s) 98
PfeI GAWTC 1 cut(s) 58
PkrI GCNGC 1 cut(s) 216
PpuMI RGGWCCY 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 77
PspN4I GGNNCC 3 cut(s) 78, 248, 295
PspPI GGNCC 4 cut(s) 77, 216, 302, 310
PspPPI RGGWCCY 1 cut(s) 77
PsuI RGATCY 1 cut(s) 293
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
SatI GCNGC 1 cut(s) 215
Sau3AI GATC 1 cut(s) 293
Sau96I GGNCC 4 cut(s) 77, 216, 302, 310
SduI GDGCHC 1 cut(s) 198
SetI ASST 5 cut(s) 82, 103, 112, 285, 394
SfaNI GCATC 1 cut(s) 140
SinI GGWCC 2 cut(s) 77, 310
SsiI CCGC 4 cut(s) 51, 214, 287, 359
TaaI ACNGT 1 cut(s) 310
TaqI TCGA 2 cut(s) 146, 235
TauI GCSGC 1 cut(s) 217
TfiI GAWTC 1 cut(s) 58
TscAI CASTG 2 cut(s) 233, 382
TseFI GTSAC 1 cut(s) 41
Tsp45I GTSAC 1 cut(s) 41
TspDTI ATGAA 2 cut(s) 316, 399
TspRI CASTG 2 cut(s) 233, 382
VneI GTGCAC 1 cut(s) 194
VpaK11BI GGWCC 2 cut(s) 77, 310
XagI CCTNNNNNAGG 1 cut(s) 105
XcmI CCANNNNNNNNNTGG 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.