Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0002275.1

Orthogroup ID

OG0005801

Physical Location & Seq

Genomic Coordinates Forward (+)

23180 .. 30860

Gene Structure 7681 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2487 bp

atgtcacagttctggaaaccaggggcggagaagcctcgcctcctcgacgacgaagaaggcggcgtcgttttcttccccacctcctccggaggaggctatgcgaatctcgagaggcagaggcagagactaccggtgttcaggtaccgcaccgccattctctactccgtcgagactcgcgccaccaccgtcgtcgtcggcgaaaccggcagcggcaaaaccactcagattcctcagtatttgaaagaagcaggttgggctgatggtggccgtataattgcttgtactcagccgagaagattggctgttcagaggtcggatcgggtccgggtcatccctgcgcgcctaatacgcacactgctaaaagctctaaaccaaaatcaaagagtagcagagttgggcagaagaatgtcacagttctggaaaccaggggcggagaagcctcgcctcctcgacgacgaagaaggcggcgtcgttttcttccccacctcctccggaggaggctatgcgaacctcgagaggcagaggcagagactaccggtgttcaggtaccgcaccgccattctctactccgtcgagactcgcgccaccaccatcgtcgtcggcgaaaccggcagcggcaaaaccactcagattcctcagtatttgaaagaagcaggttgggctgatggtggccgtgtaattgcttgtactcagccgagaagattggctgttcagtcggttgcagcacgggtggctgaagaaatgggggtcaagcttggggaggaagttggctacactattcgatttgaagatgctacaacacagggtgtgactatgataaagtttttgactgatggtgtgttactcagggaaatgatggacgatcctcttttgactaaatatagtgttgtaatggtagatgaagctcatgaaaggtctatctccacggacattttattgggtctcctgaaaaagatccaaaaacgccggcctgagctgcgcctgattatctcatctgctacaattcaagcaaagtcaatggctgctttctttcagtccagcagcaaaaggcgccgcagaccagaaggagaagagcttggaccaattatggagcctgcagtcctgtcagttgagggtagagggtttaatgtacaaattcattacgtggaggagcctgttgccgactatcttcgggctgcagtctcaactgtattattgatacatgaccaggaacccatgggagatattctggtatttcttactggtcaagatgatatcgatgctgctgcccaacttattacggaagagacccaaaaccgtagaaagaactcatcaggactgatggttttgcctctctattcgggacttccacgtgctgaccaggagctggtgttcagtcctactcctagagggaaaaggaaagtagttatatcaaccaacatagccgagacatcaataactttagagggcgtggtctatgttgttgacagtgggttctcaaagcaacggttctataacccgatatcagatattgaaaatctggtagtggcaccaatatccaaggcatctgctaggcaaaggaccggtagagctggaagagttcggcctgggaaatgttacaggctgtatacagaagagtattttgtaaaggaaatgtctgctgaggggattccagagatacaaagatcaaatcttgtttcctgtgtgatccagttaaaagccttgggaatagataatatattgggctttgattggccagcatctccatcttctgaagcaatggttcgagcacttgaagtgctttattcacttggggtcctggatgatgatgctaaacttacttcaccaactggatttcaagttgctgagtttccattggatccaatgatctcaaagatgatcttatcatctggtgaactggggtgttcagaagagattgttactattgcggctgttctctctgttcaatctatctgggcttctggttggggatcacaaaaggaacttgatgaagccaagttgaattttgctgcagctgagggtgaccatgtgagtttcctaaatgtctacaaagcatttcttcagtctggtaaatcttcacagtggtgtcataagaactttgtaaactaccaagccatgaaaaaggttcttgaaataagggaacaactgagaagaacagctcgaagattaggcattgacttgaaatcttgtgaaagagatacagtggtagtgagaaaggccattacttatggatttttcgctaatgcgtgtgtttcagaagcatcaagtcacgatggaaagtacaaaactatcagaggttctcaagaagtttatattcacccctcatctgtgctattcagagttaatcccaagtgggtggtttaccattctcttgtctcaactgaaaagcaatacatgcggaatgtcatttcgatagatccttcttggctcacagaggcagctccacatttttaccaaaagcagcgacccaatcctatgcttcactag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009506 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030054 GO:0032991 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0055044 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_22 HA2 Helicase_C OB_NTP_bind

Protein Analysis

828

Amino Acids

92.41

Weight (kDa)

9.14

Isoelectric Point (pI)

47.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_22	PF13401	193 - 321	1.1e-06	AAA domain
DEAD	PF00270	193 - 339	2.3e-06	DEAD/DEAH box helicase
Helicase_C	PF00271	399 - 527	1.2e-12	Helicase conserved C-terminal domain
WHD_HA2	PF04408	590 - 619	7.2e-06	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	620 - 678	1.5e-09	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	737 - 816	8.1e-23	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005801)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18465
fragaria_vesca	FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450
malus_domestica	MD05G1305600.v1.1
prunus_persica	Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1
pyrus_communis	pycom05g28290
rosa_chinensis	RchiOBHm_Chr2g0158101 RchiOBHm_Chr5g0009991
rosa_laevigata	RLG00000031717
rosa_multiflora	Rmu_sc0001648.1_g000001 Rmu_sc0002275.1_g000005
rosa_roxburghii	Rroxscaffold_1G00066170
rosa_rugosa	Rorug04G0448300 Rorug04G0448400 Rorug04G0448500
rosa_samantha	Rh5AG079200 Rh5BG074000 Rh5CG086600 Rh5DG074700
rosa_wichuraiana	Rw5G007330 Rw7G028410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	239, 644
Acc65I	GGTACC	2 cut(s)	141, 546
AccB1I	GGYRCC	4 cut(s)	141, 546, 1050, 1528
AccB7I	CCANNNNNTGG	1 cut(s)	1366
AccI	GTMKAC	2 cut(s)	1607, 2046
AccII	CGCG	3 cut(s)	177, 340, 582
AccIII	TCCGGA	2 cut(s)	86, 491
AclWI	GGATC	8 cut(s)	324, 857, 949, 1681, 1853, 1866, 1978, 2411
AcoI	YGGCCR	3 cut(s)	265, 670, 1733
AcsI	RAATTY	2 cut(s)	1134, 2002
AcuI	CTGAAG	3 cut(s)	756, 1773, 2045
AcvI	CACGTG	1 cut(s)	1352
AcyI	GRCGYC	3 cut(s)	63, 468, 1051
AdeI	CACNNNGTG	1 cut(s)	806
AfaI	GTAC	6 cut(s)	143, 283, 548, 688, 1131, 2282
AfiI	CCNNNNNNNGG	1 cut(s)	1366
AgeI	ACCGGT	3 cut(s)	130, 535, 1562
AjnI	CCWGG	6 cut(s)	19, 424, 1206, 1359, 1585, 1797
AleI	CACNNNNGTG	1 cut(s)	2083
AloI	GAACNNNNNNTCC	2 cut(s)	1355, 1387
Alw21I	GWGCWC	1 cut(s)	1771
Alw26I	GTCTC	9 cut(s)	118, 164, 523, 569, 947, 1186, 1280, 1421, 2380
AlwI	GGATC	8 cut(s)	324, 857, 949, 1681, 1853, 1866, 1978, 2411
AlwNI	CAGNNNCTG	1 cut(s)	1366
Ama87I	CYCGRG	2 cut(s)	107, 512
Aor13HI	TCCGGA	2 cut(s)	86, 491
AoxI	GGCC	6 cut(s)	265, 670, 968, 1583, 1733, 2217
ApoI	RAATTY	2 cut(s)	1134, 2002
AsiGI	ACCGGT	3 cut(s)	130, 535, 1562
Asp718I	GGTACC	2 cut(s)	141, 546
AspLEI	GCGC	6 cut(s)	179, 340, 342, 584, 981, 1053
AspS9I	GGNCC	4 cut(s)	322, 1079, 1560, 1795
AsuC2I	CCSGG	1 cut(s)	326
AsuHPI	GGTGA	4 cut(s)	1815, 1904, 2033, 2309
AvaI	CYCGRG	2 cut(s)	107, 512
AvaII	GGWCC	4 cut(s)	322, 1079, 1560, 1795
BaeI	ACNNNNGTAYC	2 cut(s)	2190, 2223
BalI	TGGCCA	1 cut(s)	1735
BamHI	GGATCC	1 cut(s)	1858
BanI	GGYRCC	4 cut(s)	141, 546, 1050, 1528
BbrPI	CACGTG	1 cut(s)	1352
Bbv12I	GWGCWC	1 cut(s)	1771
BbvCI	CCTCAGC	2 cut(s)	1641, 2016
BccI	CCATC	8 cut(s)	254, 599, 659, 827, 850, 1315, 1753, 2267
BceAI	ACGGC	2 cut(s)	252, 657
BcgI	CGANNNNNNTGC	2 cut(s)	1247, 1281
BciT130I	CCWGG	6 cut(s)	21, 426, 1208, 1361, 1587, 1799
BcnI	CCSGG	1 cut(s)	326
BcoDI	GTCTC	9 cut(s)	118, 164, 523, 569, 947, 1186, 1280, 1421, 2380
BfaI	CTAG	3 cut(s)	1386, 1551, 2485
BfmI	CTRYAG	3 cut(s)	1095, 1176, 2010
BfoI	RGCGCY	1 cut(s)	1054
BfuAI	ACCTGC	2 cut(s)	239, 644
Bme1390I	CCNGG	7 cut(s)	21, 326, 426, 1208, 1361, 1587, 1799
Bme18I	GGWCC	4 cut(s)	322, 1079, 1560, 1795
BmeT110I	CYCGRG	2 cut(s)	107, 512
BmgT120I	GGNCC	4 cut(s)	322, 1079, 1560, 1795
BmrFI	CCNGG	7 cut(s)	21, 326, 426, 1208, 1361, 1587, 1799
BmrI	ACTGGG	1 cut(s)	1907
BmsI	GCATC	6 cut(s)	781, 1249, 1553, 1748, 1798, 2270
BmuI	ACTGGG	1 cut(s)	1907
Bpu10I	CCTNAGC	3 cut(s)	972, 1641, 2016
BpuEI	CTTGAG	1 cut(s)	2286
BpuMI	CCSGG	1 cut(s)	326
Bsa29I	ATCGAT	1 cut(s)	1257
BsaAI	YACGTR	2 cut(s)	1144, 1352
BsaHI	GRCGYC	3 cut(s)	63, 468, 1051
BsaI	GGTCTC	2 cut(s)	947, 1280
BsaJI	CCNNGG	7 cut(s)	20, 425, 924, 1215, 1539, 1586, 1701
BsaWI	WCCGGW	5 cut(s)	86, 130, 491, 535, 1562
BsaXI	ACNNNNNCTCC	2 cut(s)	1355, 1385
Bsc4I	CCNNNNNNNGG	1 cut(s)	1366
Bse118I	RCCGGY	6 cut(s)	130, 203, 535, 608, 966, 1562
Bse1I	ACTGG	4 cut(s)	1246, 1690, 1834, 1902
Bse3DI	GCAATG	1 cut(s)	1764
BseAI	TCCGGA	2 cut(s)	86, 491
BseBI	CCWGG	6 cut(s)	21, 426, 1208, 1361, 1587, 1799
BseCI	ATCGAT	1 cut(s)	1257
BseDI	CCNNGG	7 cut(s)	20, 425, 924, 1215, 1539, 1586, 1701
BseGI	GGATG	2 cut(s)	330, 1807
BseLI	CCNNNNNNNGG	1 cut(s)	1366
BseMI	GCAATG	1 cut(s)	1764
BseNI	ACTGG	4 cut(s)	1246, 1690, 1834, 1902
BsePI	GCGCGC	1 cut(s)	338
BseRI	GAGGAG	7 cut(s)	32, 73, 105, 437, 478, 510, 1163
Bsh1236I	CGCG	3 cut(s)	177, 340, 582
BshFI	GGCC	6 cut(s)	267, 672, 970, 1585, 1735, 2219
BshNI	GGYRCC	4 cut(s)	141, 546, 1050, 1528
BshTI	ACCGGT	3 cut(s)	130, 535, 1562
BshVI	ATCGAT	1 cut(s)	1257
BsiHKAI	GWGCWC	1 cut(s)	1771
BsiHKCI	CYCGRG	2 cut(s)	107, 512
BsiSI	CCGG	9 cut(s)	87, 131, 204, 325, 492, 536, 609, 967, 1563
BslFI	GGGAC	1 cut(s)	1356
BslI	CCNNNNNNNGG	1 cut(s)	1366
BsmAI	GTCTC	9 cut(s)	118, 164, 523, 569, 947, 1186, 1280, 1421, 2380
BsmFI	GGGAC	1 cut(s)	1356
BsnI	GGCC	6 cut(s)	267, 672, 970, 1585, 1735, 2219
Bso31I	GGTCTC	2 cut(s)	947, 1280
BsoBI	CYCGRG	2 cut(s)	107, 512
Bsp1286I	GDGCHC	1 cut(s)	1771
Bsp13I	TCCGGA	2 cut(s)	86, 491
Bsp1407I	TGTACA	1 cut(s)	1129
Bsp19I	CCATGG	1 cut(s)	1215
BspANI	GGCC	6 cut(s)	267, 672, 970, 1585, 1735, 2219
BspDI	ATCGAT	1 cut(s)	1257
BspEI	TCCGGA	2 cut(s)	86, 491
BspFNI	CGCG	3 cut(s)	177, 340, 582
BspHI	TCATGA	1 cut(s)	907
BspMAI	CTGCAG	3 cut(s)	1099, 1180, 2014
BspMI	ACCTGC	2 cut(s)	239, 644
BspPI	GGATC	8 cut(s)	324, 857, 949, 1681, 1853, 1866, 1978, 2411
BspQI	GCTCTTC	1 cut(s)	1065
BspT107I	GGYRCC	4 cut(s)	141, 546, 1050, 1528
BspTNI	GGTCTC	2 cut(s)	947, 1280
BsrDI	GCAATG	1 cut(s)	1764
BsrFI	RCCGGY	6 cut(s)	130, 203, 535, 608, 966, 1562
BsrGI	TGTACA	1 cut(s)	1129
BsrI	ACTGG	4 cut(s)	1246, 1690, 1834, 1902
BssAI	RCCGGY	6 cut(s)	130, 203, 535, 608, 966, 1562
BssECI	CCNNGG	7 cut(s)	20, 425, 924, 1215, 1539, 1586, 1701
BssHII	GCGCGC	1 cut(s)	338
BssNAI	GTATAC	1 cut(s)	1608
BssNI	GRCGYC	3 cut(s)	63, 468, 1051
BssT1I	CCWWGG	3 cut(s)	1215, 1539, 1701
Bst1107I	GTATAC	1 cut(s)	1608
Bst2UI	CCWGG	6 cut(s)	21, 426, 1208, 1361, 1587, 1799
Bst4CI	ACNGT	9 cut(s)	9, 187, 414, 1189, 1298, 1469, 1488, 2082, 2203
Bst6I	CTCTTC	5 cut(s)	1065, 1278, 1570, 1608, 1905
BstACI	GRCGYC	3 cut(s)	63, 468, 1051
BstAPI	GCANNNNNTGC	1 cut(s)	2395
BstAUI	TGTACA	1 cut(s)	1129
BstBAI	YACGTR	2 cut(s)	1144, 1352
BstC8I	GCNNGC	4 cut(s)	340, 968, 1095, 1737
BstDSI	CCRYGG	2 cut(s)	924, 1215
BstEII	GGTNACC	1 cut(s)	2021
BstF5I	GGATG	2 cut(s)	330, 1807
BstFNI	CGCG	3 cut(s)	177, 340, 582
BstH2I	RGCGCY	1 cut(s)	1054
BstHHI	GCGC	6 cut(s)	179, 340, 342, 584, 981, 1053
BstMAI	GTCTC	9 cut(s)	118, 164, 523, 569, 947, 1186, 1280, 1421, 2380
BstMWI	GCNNNNNNNGC	9 cut(s)	204, 254, 348, 609, 659, 731, 976, 1050, 2395
BstNI	CCWGG	6 cut(s)	21, 426, 1208, 1361, 1587, 1799
BstNSI	RCATGY	1 cut(s)	2398
BstPI	GGTNACC	1 cut(s)	2021
BstSCI	CCNGG	7 cut(s)	19, 324, 424, 1206, 1359, 1585, 1797
BstSFI	CTRYAG	3 cut(s)	1095, 1176, 2010
BstUI	CGCG	3 cut(s)	177, 340, 582
BstX2I	RGATCY	3 cut(s)	954, 1858, 2416
BstXI	CCANNNNNNTGG	1 cut(s)	2356
BstYI	RGATCY	3 cut(s)	954, 1858, 2416
BstZ17I	GTATAC	1 cut(s)	1608
Bsu15I	ATCGAT	1 cut(s)	1257
BsuRI	GGCC	6 cut(s)	267, 672, 970, 1585, 1735, 2219
BsuTUI	ATCGAT	1 cut(s)	1257
BtgI	CCRYGG	2 cut(s)	924, 1215
BtsCI	GGATG	2 cut(s)	330, 1807
BtsI	GCAGTG	1 cut(s)	353
BtsIMutI	CAGTG	4 cut(s)	353, 1474, 2087, 2208
BveI	ACCTGC	2 cut(s)	239, 644
Cac8I	GCNNGC	4 cut(s)	340, 968, 1095, 1737
CaiI	CAGNNNCTG	1 cut(s)	1366
CciI	TCATGA	1 cut(s)	907
CfoI	GCGC	6 cut(s)	179, 340, 342, 584, 981, 1053
Cfr10I	RCCGGY	6 cut(s)	130, 203, 535, 608, 966, 1562
Cfr13I	GGNCC	4 cut(s)	322, 1079, 1560, 1795
ClaI	ATCGAT	1 cut(s)	1257
CseI	GACGC	2 cut(s)	52, 457
Csp6I	GTAC	6 cut(s)	142, 282, 547, 687, 1130, 2281
CspAI	ACCGGT	3 cut(s)	130, 535, 1562
CviAII	CATG	6 cut(s)	908, 1202, 1216, 2027, 2116, 2395
CviQI	GTAC	6 cut(s)	142, 282, 547, 687, 1130, 2281
DinI	GGCGCC	1 cut(s)	1052
DraIII	CACNNNGTG	1 cut(s)	806
EaeI	YGGCCR	3 cut(s)	265, 670, 1733
Eam1104I	CTCTTC	5 cut(s)	1065, 1278, 1570, 1608, 1905
EarI	CTCTTC	5 cut(s)	1065, 1278, 1570, 1608, 1905
EciI	GGCGGA	2 cut(s)	41, 446
Eco130I	CCWWGG	3 cut(s)	1215, 1539, 1701
Eco31I	GGTCTC	2 cut(s)	947, 1280
Eco32I	GATATC	2 cut(s)	1255, 1503
Eco47I	GGWCC	4 cut(s)	322, 1079, 1560, 1795
Eco57I	CTGAAG	3 cut(s)	756, 1773, 2045
Eco72I	CACGTG	1 cut(s)	1352
Eco88I	CYCGRG	2 cut(s)	107, 512
Eco91I	GGTNACC	1 cut(s)	2021
EcoO109I	RGGNCCY	1 cut(s)	1795
EcoO65I	GGTNACC	1 cut(s)	2021
EcoRII	CCWGG	6 cut(s)	19, 424, 1206, 1359, 1585, 1797
EcoRV	GATATC	2 cut(s)	1255, 1503
EcoT14I	CCWWGG	3 cut(s)	1215, 1539, 1701
EgeI	GGCGCC	1 cut(s)	1052
EheI	GGCGCC	1 cut(s)	1052
ErhI	CCWWGG	3 cut(s)	1215, 1539, 1701
FaeI	CATG	6 cut(s)	911, 1205, 1219, 2030, 2119, 2398
FalI	AAGNNNNNCTT	4 cut(s)	1865, 1897, 2043, 2075
FaqI	GGGAC	1 cut(s)	1356
FatI	CATG	6 cut(s)	907, 1201, 1215, 2026, 2115, 2394
FblI	GTMKAC	2 cut(s)	1607, 2046
FokI	GGATG	2 cut(s)	317, 1814
FspBI	CTAG	3 cut(s)	1386, 1551, 2485
GlaI	GCGC	6 cut(s)	178, 339, 341, 583, 980, 1052
HaeII	RGCGCY	1 cut(s)	1054
HaeIII	GGCC	6 cut(s)	267, 672, 970, 1585, 1735, 2219
HapII	CCGG	9 cut(s)	87, 131, 204, 325, 492, 536, 609, 967, 1563
HgaI	GACGC	2 cut(s)	52, 457
HhaI	GCGC	6 cut(s)	179, 340, 342, 584, 981, 1053
Hin1I	GRCGYC	3 cut(s)	63, 468, 1051
Hin1II	CATG	6 cut(s)	911, 1205, 1219, 2030, 2119, 2398
Hin6I	GCGC	6 cut(s)	177, 338, 340, 582, 979, 1051
HinP1I	GCGC	6 cut(s)	177, 338, 340, 582, 979, 1051
HincII	GTYRAC	1 cut(s)	1465
HindII	GTYRAC	1 cut(s)	1465
HindIII	AAGCTT	1 cut(s)	752
HinfI	GANTC	6 cut(s)	103, 172, 226, 577, 631, 1648
HpaII	CCGG	9 cut(s)	87, 131, 204, 325, 492, 536, 609, 967, 1563
HphI	GGTGA	4 cut(s)	1815, 1904, 2033, 2309
Hpy166II	GTNNAC	6 cut(s)	1465, 1608, 1895, 2047, 2104, 2362
Hpy8I	GTNNAC	6 cut(s)	1465, 1608, 1895, 2047, 2104, 2362
HpyAV	CCTTC	4 cut(s)	50, 455, 1058, 2430
HpyCH4III	ACNGT	9 cut(s)	9, 187, 414, 1189, 1298, 1469, 1488, 2082, 2203
HpyCH4IV	ACGT	2 cut(s)	1143, 1351
HpyCH4V	TGCA	4 cut(s)	722, 1097, 1178, 2012
HpyF10VI	GCNNNNNNNGC	9 cut(s)	204, 254, 348, 609, 659, 731, 976, 1050, 2395
HpySE526I	ACGT	2 cut(s)	1143, 1351
Hsp92I	GRCGYC	3 cut(s)	63, 468, 1051
Hsp92II	CATG	6 cut(s)	911, 1205, 1219, 2030, 2119, 2398
HspAI	GCGC	6 cut(s)	177, 338, 340, 582, 979, 1051
KasI	GGCGCC	1 cut(s)	1050
Kpn2I	TCCGGA	2 cut(s)	86, 491
KpnI	GGTACC	2 cut(s)	145, 550
KroI	GCCGGC	1 cut(s)	966
KroNI	GCCGGC	1 cut(s)	968
LguI	GCTCTTC	1 cut(s)	1065
LmnI	GCTCC	4 cut(s)	1090, 1150, 1363, 2446
LweI	GCATC	6 cut(s)	781, 1249, 1553, 1748, 1798, 2270
MaeI	CTAG	3 cut(s)	1386, 1551, 2485
MaeII	ACGT	2 cut(s)	1143, 1351
MaeIII	GTNAC	8 cut(s)	3, 408, 808, 840, 1595, 1918, 2021, 2267
MflI	RGATCY	3 cut(s)	954, 1858, 2416
MhlI	GDGCHC	1 cut(s)	1771
MlsI	TGGCCA	1 cut(s)	1735
MluCI	AATT	6 cut(s)	273, 678, 1002, 1083, 1134, 2002
MluNI	TGGCCA	1 cut(s)	1735
Mly113I	GGCGCC	1 cut(s)	1051
MlyI	GAGTC	2 cut(s)	166, 571
MmeI	TCCRAC	1 cut(s)	294
Mox20I	TGGCCA	1 cut(s)	1735
MroI	TCCGGA	2 cut(s)	86, 491
MroNI	GCCGGC	1 cut(s)	966
MscI	TGGCCA	1 cut(s)	1735
MseI	TTAA	3 cut(s)	1125, 1694, 2343
MslI	CAYNNNNRTG	1 cut(s)	2083
Msp20I	TGGCCA	1 cut(s)	1735
MspA1I	CMGCKG	3 cut(s)	210, 615, 2015
MspI	CCGG	9 cut(s)	87, 131, 204, 325, 492, 536, 609, 967, 1563
MspR9I	CCNGG	7 cut(s)	21, 326, 426, 1208, 1361, 1587, 1799
MvaI	CCWGG	6 cut(s)	21, 426, 1208, 1361, 1587, 1799
MvnI	CGCG	3 cut(s)	177, 340, 582
MwoI	GCNNNNNNNGC	9 cut(s)	204, 254, 348, 609, 659, 731, 976, 1050, 2395
NaeI	GCCGGC	1 cut(s)	968
NarI	GGCGCC	1 cut(s)	1051
NciI	CCSGG	1 cut(s)	326
NcoI	CCATGG	1 cut(s)	1215
NgoMIV	GCCGGC	1 cut(s)	966
NlaIII	CATG	6 cut(s)	911, 1205, 1219, 2030, 2119, 2398
NmeAIII	GCCGAG	3 cut(s)	315, 720, 1450
NmuCI	GTSAC	5 cut(s)	3, 408, 808, 2021, 2267
NspI	RCATGY	1 cut(s)	2398
OliI	CACNNNNGTG	1 cut(s)	2083
PaeR7I	CTCGAG	2 cut(s)	107, 512
PagI	TCATGA	1 cut(s)	907
PauI	GCGCGC	1 cut(s)	338
PciSI	GCTCTTC	1 cut(s)	1065
PcsI	WCGNNNNNNNCGW	2 cut(s)	195, 600
PdiI	GCCGGC	1 cut(s)	968
PfeI	GAWTC	4 cut(s)	103, 226, 631, 1648
PflMI	CCANNNNNTGG	1 cut(s)	1366
PfoI	TCCNGGA	1 cut(s)	1797
PinAI	ACCGGT	3 cut(s)	130, 535, 1562
PleI	GAGTC	2 cut(s)	166, 571
PluTI	GGCGCC	1 cut(s)	1054
PmaCI	CACGTG	1 cut(s)	1352
PmlI	CACGTG	1 cut(s)	1352
PpsI	GAGTC	2 cut(s)	166, 571
Ppu21I	YACGTR	2 cut(s)	1144, 1352
PpuMI	RGGWCCY	1 cut(s)	1795
Psp5II	RGGWCCY	1 cut(s)	1795
Psp6I	CCWGG	6 cut(s)	19, 424, 1206, 1359, 1585, 1797
PspCI	CACGTG	1 cut(s)	1352
PspEI	GGTNACC	1 cut(s)	2021
PspGI	CCWGG	6 cut(s)	19, 424, 1206, 1359, 1585, 1797
PspPI	GGNCC	4 cut(s)	322, 1079, 1560, 1795
PspPPI	RGGWCCY	1 cut(s)	1795
PstI	CTGCAG	3 cut(s)	1099, 1180, 2014
PstNI	CAGNNNCTG	1 cut(s)	1366
PsuI	RGATCY	3 cut(s)	954, 1858, 2416
PteI	GCGCGC	1 cut(s)	338
PvuII	CAGCTG	1 cut(s)	2015
RsaI	GTAC	6 cut(s)	143, 283, 548, 688, 1131, 2282
RsaNI	GTAC	6 cut(s)	142, 282, 547, 687, 1130, 2281
RseI	CAYNNNNRTG	1 cut(s)	2083
SapI	GCTCTTC	1 cut(s)	1065
SaqAI	TTAA	3 cut(s)	1125, 1694, 2343
Sau96I	GGNCC	4 cut(s)	322, 1079, 1560, 1795
SchI	GAGTC	2 cut(s)	166, 571
ScrFI	CCNGG	7 cut(s)	21, 326, 426, 1208, 1361, 1587, 1799
SduI	GDGCHC	1 cut(s)	1771
SfaNI	GCATC	6 cut(s)	781, 1249, 1553, 1748, 1798, 2270
SfcI	CTRYAG	3 cut(s)	1095, 1176, 2010
SfoI	GGCGCC	1 cut(s)	1052
Sfr274I	CTCGAG	2 cut(s)	107, 512
SinI	GGWCC	4 cut(s)	322, 1079, 1560, 1795
SlaI	CTCGAG	2 cut(s)	107, 512
SmiMI	CAYNNNNRTG	1 cut(s)	2083
SmlI	CTYRAG	3 cut(s)	107, 512, 2301
SmoI	CTYRAG	3 cut(s)	107, 512, 2301
Sse9I	AATT	6 cut(s)	273, 678, 1002, 1083, 1134, 2002
SspDI	GGCGCC	1 cut(s)	1050
SspMI	CTAG	3 cut(s)	1386, 1551, 2485
StyD4I	CCNGG	7 cut(s)	19, 324, 424, 1206, 1359, 1585, 1797
StyI	CCWWGG	3 cut(s)	1215, 1539, 1701
TaaI	ACNGT	9 cut(s)	9, 187, 414, 1189, 1298, 1469, 1488, 2082, 2203
TaiI	ACGT	2 cut(s)	1146, 1354
TasI	AATT	6 cut(s)	273, 678, 1002, 1083, 1134, 2002
TatI	WGTACW	4 cut(s)	281, 686, 1129, 2280
TauI	GCSGC	6 cut(s)	63, 213, 468, 618, 1056, 1931
TfiI	GAWTC	4 cut(s)	103, 226, 631, 1648
Tru1I	TTAA	3 cut(s)	1125, 1694, 2343
Tru9I	TTAA	3 cut(s)	1125, 1694, 2343
TscAI	CASTG	4 cut(s)	360, 1474, 2087, 2208
TseFI	GTSAC	5 cut(s)	3, 408, 808, 2021, 2267
Tsp45I	GTSAC	5 cut(s)	3, 408, 808, 2021, 2267
TspDTI	ATGAA	5 cut(s)	915, 924, 1127, 2004, 2132
TspGWI	ACGGA	4 cut(s)	154, 559, 941, 1295
TspRI	CASTG	4 cut(s)	360, 1474, 2087, 2208
Van91I	CCANNNNNTGG	1 cut(s)	1366
VpaK11BI	GGWCC	4 cut(s)	322, 1079, 1560, 1795
XapI	RAATTY	2 cut(s)	1134, 2002
XceI	RCATGY	1 cut(s)	2398
XhoI	CTCGAG	2 cut(s)	107, 512
XmiI	GTMKAC	2 cut(s)	1607, 2046
XspI	CTAG	3 cut(s)	1386, 1551, 2485

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.