Rmu_sc0002435.1_g000012

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002435.1
OG0009745
Physical Location & Seq
Forward (+)
47879 .. 48814
936 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 936 bp
atggccgccagattggtatctcgctcctctcttcacaaccaatccaatcccttctcccttttcttcaccaagcttcacacaaccacagctccaatcaaccacacccacaaactcatctctttctcttcttcttctccttcatccacaacaacccataacagcaggtctcttcttcctccgccaccattctcttcttttcgcctcttcagctcctccgaacccaacaccaagcgttccaccctcgtcaatttctctctcccctccgacgacgaagacgacgacgccgatcagaaaccaaacgacaagtcgtcatcgtcgtcgtcgtccctccctcctccctacgacccattcaacaagaagccggcgatcgaagacccagaagacccaaaagacttgcaggaagtgttccacaagatgcgcacagatgggctccaaaacaatgcagtcaagatgttcgacgctttgtccaaggacggcctcacccacgaggccttagagctcttcgcccagatcaaagacaagggccacatgcccgacgtggtggcccacaccgccgtcatcgaggcctacgccgccgccggaaagcccaaggacgctctcaaggtgtacatgcggatgttggcgtgtggggttgcaccaaatgcctacacttatagcgtgctcatcaaggcactcgctgccgacgccagtttcgttggggatgcgaagaagtatctgatagagatgatggggaagggaatgcggcccaatgccgggacgtgcgtggcggtgtttgaggcgtttgcggccgaggagagggtggaggatgggagggagttgctggaggagatgaaagggaaagggtttgtggcggacgagcaggcggtgagggaggtgctcaagggtagaagaggagctgtggttagaagtgtcatcaaccttctctttggaaagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 Ribosomal_L15e

Protein Analysis

311

Amino Acids

34.01

Weight (kDa)

6.76

Isoelectric Point (pI)

56.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 156 - 294 1.3e-06 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 157 - 267 3e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 167 - 225 8e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 178 - 226 5.6e-14 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 419
Acc36I ACCTGC 1 cut(s) 153
AcoI YGGCCR 2 cut(s) 3, 786
AcuI CTGAAG 1 cut(s) 190
AcyI GRCGYC 2 cut(s) 282, 684
AfaI GTAC 1 cut(s) 608
AfiI CCNNNNNNNGG 3 cut(s) 752, 753, 795
AgsI TTSAA 1 cut(s) 352
AhdI GACNNNNNGTC 2 cut(s) 307, 463
AjiI CACGTC 2 cut(s) 538, 759
AluBI AGCT 5 cut(s) 73, 89, 210, 499, 896
AluI AGCT 5 cut(s) 73, 89, 210, 499, 896
Alw21I GWGCWC 3 cut(s) 501, 663, 879
Alw26I GTCTC 1 cut(s) 171
AoxI GGCC 8 cut(s) 3, 475, 489, 523, 543, 564, 743, 786
ApeKI GCWGC 1 cut(s) 677
ArsI GACNNNNNNTTYG 4 cut(s) 290, 322, 674, 706
AspLEI GCGC 1 cut(s) 420
AspS9I GGNCC 3 cut(s) 523, 544, 744
AsuC2I CCSGG 1 cut(s) 754
AsuHPI GGTGA 3 cut(s) 58, 472, 877
BanII GRGCYC 2 cut(s) 432, 501
BauI CACGAG 1 cut(s) 485
BbsI GAAGAC 3 cut(s) 279, 378, 387
Bbv12I GWGCWC 3 cut(s) 501, 663, 879
BbvI GCAGC 1 cut(s) 664
BccI CCATC 3 cut(s) 419, 721, 800
BceAI ACGGC 2 cut(s) 490, 539
BcnI CCSGG 1 cut(s) 754
BcoDI GTCTC 1 cut(s) 171
BfuAI ACCTGC 1 cut(s) 153
BisI GCNGC 6 cut(s) 6, 573, 576, 678, 743, 786
BlsI GCNGC 6 cut(s) 7, 574, 577, 679, 744, 787
Bme1390I CCNGG 1 cut(s) 754
BmeRI GACNNNNNGTC 2 cut(s) 307, 463
BmgBI CACGTC 2 cut(s) 538, 759
BmgT120I GGNCC 3 cut(s) 523, 544, 744
BmiI GGNNCC 1 cut(s) 431
BmrFI CCNGG 1 cut(s) 754
BmsI GCATC 2 cut(s) 405, 691
BpiI GAAGAC 3 cut(s) 279, 378, 387
BpmI CTGGAG 1 cut(s) 842
BpuEI CTTGAG 2 cut(s) 584, 863
BpuMI CCSGG 1 cut(s) 754
BsaBI GATNNNNATC 1 cut(s) 16
BsaHI GRCGYC 2 cut(s) 282, 684
BsaI GGTCTC 1 cut(s) 171
BsaJI CCNNGG 3 cut(s) 468, 588, 789
BsaXI ACNNNNNCTCC 4 cut(s) 73, 103, 885, 915
Bsc4I CCNNNNNNNGG 3 cut(s) 752, 753, 795
Bse118I RCCGGY 1 cut(s) 361
Bse1I ACTGG 1 cut(s) 687
Bse8I GATNNNNATC 1 cut(s) 16
BseDI CCNNGG 3 cut(s) 468, 588, 789
BseGI GGATG 4 cut(s) 140, 621, 706, 811
BseJI GATNNNNATC 1 cut(s) 16
BseLI CCNNNNNNNGG 3 cut(s) 752, 753, 795
BseNI ACTGG 1 cut(s) 687
BseRI GAGGAG 6 cut(s) 16, 202, 324, 806, 839, 906
BseX3I CGGCCG 1 cut(s) 786
BseXI GCAGC 1 cut(s) 664
Bsh1285I CGRYCG 2 cut(s) 369, 789
BshFI GGCC 8 cut(s) 5, 477, 491, 525, 545, 566, 745, 788
BsiEI CGRYCG 2 cut(s) 369, 789
BsiHKAI GWGCWC 3 cut(s) 501, 663, 879
BsiSI CCGG 3 cut(s) 362, 579, 753
BslFI GGGAC 2 cut(s) 310, 769
BslI CCNNNNNNNGG 3 cut(s) 752, 753, 795
BsmAI GTCTC 1 cut(s) 171
BsmFI GGGAC 2 cut(s) 310, 769
BsmI GAATGC 1 cut(s) 744
BsnI GGCC 8 cut(s) 5, 477, 491, 525, 545, 566, 745, 788
Bso31I GGTCTC 1 cut(s) 171
Bsp1286I GDGCHC 4 cut(s) 432, 501, 663, 879
Bsp1407I TGTACA 1 cut(s) 606
Bsp143I GATC 3 cut(s) 286, 366, 510
BspANI GGCC 8 cut(s) 5, 477, 491, 525, 545, 566, 745, 788
BspLI GGNNCC 1 cut(s) 431
BspMI ACCTGC 1 cut(s) 153
BspQI GCTCTTC 1 cut(s) 506
BspTNI GGTCTC 1 cut(s) 171
BsrFI RCCGGY 1 cut(s) 361
BsrGI TGTACA 1 cut(s) 606
BsrI ACTGG 1 cut(s) 687
BssAI RCCGGY 1 cut(s) 361
BssECI CCNNGG 3 cut(s) 468, 588, 789
BssMI GATC 3 cut(s) 286, 366, 510
BssNI GRCGYC 2 cut(s) 282, 684
BssSI CACGAG 1 cut(s) 485
BssT1I CCWWGG 2 cut(s) 468, 588
Bst2BI CACGAG 1 cut(s) 485
Bst6I CTCTTC 7 cut(s) 36, 130, 174, 196, 209, 506, 883
BstACI GRCGYC 2 cut(s) 282, 684
BstAPI GCANNNNNTGC 2 cut(s) 641, 677
BstAUI TGTACA 1 cut(s) 606
BstC8I GCNNGC 3 cut(s) 363, 659, 861
BstDEI CTNAG 1 cut(s) 493
BstF5I GGATG 4 cut(s) 140, 621, 706, 811
BstHHI GCGC 1 cut(s) 420
BstKTI GATC 3 cut(s) 289, 369, 513
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 3 cut(s) 286, 366, 510
BstMCI CGRYCG 2 cut(s) 369, 789
BstMWI GCNNNNNNNGC 7 cut(s) 207, 551, 572, 641, 677, 683, 785
BstNSI RCATGY 2 cut(s) 532, 613
BstSCI CCNGG 1 cut(s) 752
BstV1I GCAGC 1 cut(s) 664
BstV2I GAAGAC 3 cut(s) 279, 378, 387
BstZI CGGCCG 1 cut(s) 786
BsuRI GGCC 8 cut(s) 5, 477, 491, 525, 545, 566, 745, 788
BtrI CACGTC 2 cut(s) 538, 759
BtsCI GGATG 4 cut(s) 140, 621, 706, 811
BveI ACCTGC 1 cut(s) 153
Cac8I GCNNGC 3 cut(s) 363, 659, 861
CfoI GCGC 1 cut(s) 420
Cfr10I RCCGGY 1 cut(s) 361
Cfr13I GGNCC 3 cut(s) 523, 544, 744
CseI GACGC 4 cut(s) 290, 467, 602, 692
Csp6I GTAC 1 cut(s) 607
CviAII CATG 2 cut(s) 529, 610
CviQI GTAC 1 cut(s) 607
DdeI CTNAG 1 cut(s) 493
DpnI GATC 3 cut(s) 288, 368, 512
DpnII GATC 3 cut(s) 286, 366, 510
DriI GACNNNNNGTC 2 cut(s) 307, 463
EaeI YGGCCR 2 cut(s) 3, 786
EagI CGGCCG 1 cut(s) 786
Eam1104I CTCTTC 7 cut(s) 36, 130, 174, 196, 209, 506, 883
Eam1105I GACNNNNNGTC 2 cut(s) 307, 463
EarI CTCTTC 7 cut(s) 36, 130, 174, 196, 209, 506, 883
EciI GGCGGA 2 cut(s) 168, 866
Ecl136II GAGCTC 1 cut(s) 499
EclXI CGGCCG 1 cut(s) 786
Eco130I CCWWGG 2 cut(s) 468, 588
Eco147I AGGCCT 2 cut(s) 491, 566
Eco24I GRGCYC 2 cut(s) 432, 501
Eco31I GGTCTC 1 cut(s) 171
Eco52I CGGCCG 1 cut(s) 786
Eco53kI GAGCTC 1 cut(s) 499
Eco57I CTGAAG 1 cut(s) 190
EcoICRI GAGCTC 1 cut(s) 499
EcoT14I CCWWGG 2 cut(s) 468, 588
EcoT38I GRGCYC 2 cut(s) 432, 501
ErhI CCWWGG 2 cut(s) 468, 588
FaeI CATG 2 cut(s) 532, 613
FaiI YATR 4 cut(s) 156, 530, 611, 654
FaqI GGGAC 2 cut(s) 310, 769
FatI CATG 2 cut(s) 528, 609
Fnu4HI GCNGC 6 cut(s) 6, 573, 576, 678, 743, 786
FokI GGATG 4 cut(s) 127, 628, 713, 818
FriOI GRGCYC 2 cut(s) 432, 501
Fsp4HI GCNGC 6 cut(s) 6, 573, 576, 678, 743, 786
FspAI RTGCGCAY 1 cut(s) 419
FspI TGCGCA 1 cut(s) 419
GlaI GCGC 1 cut(s) 419
GluI GCNGC 6 cut(s) 6, 573, 576, 678, 743, 786
GsuI CTGGAG 1 cut(s) 842
HaeIII GGCC 8 cut(s) 5, 477, 491, 525, 545, 566, 745, 788
HapII CCGG 3 cut(s) 362, 579, 753
HgaI GACGC 4 cut(s) 290, 467, 602, 692
HhaI GCGC 1 cut(s) 420
Hin1I GRCGYC 2 cut(s) 282, 684
Hin1II CATG 2 cut(s) 532, 613
Hin6I GCGC 1 cut(s) 418
HinP1I GCGC 1 cut(s) 418
HindIII AAGCTT 1 cut(s) 71
HpaII CCGG 3 cut(s) 362, 579, 753
HphI GGTGA 3 cut(s) 58, 472, 877
Hpy166II GTNNAC 1 cut(s) 607
Hpy188I TCNGA 4 cut(s) 217, 265, 291, 717
Hpy188III TCNNGA 1 cut(s) 448
Hpy8I GTNNAC 1 cut(s) 607
HpyAV CCTTC 4 cut(s) 61, 147, 727, 929
HpyCH4IV ACGT 2 cut(s) 537, 758
HpyCH4V TGCA 3 cut(s) 397, 443, 635
HpyF10VI GCNNNNNNNGC 7 cut(s) 207, 551, 572, 641, 677, 683, 785
HpyF3I CTNAG 1 cut(s) 493
HpySE526I ACGT 2 cut(s) 537, 758
Hsp92I GRCGYC 2 cut(s) 282, 684
Hsp92II CATG 2 cut(s) 532, 613
HspAI GCGC 1 cut(s) 418
KroI GCCGGC 1 cut(s) 361
KroNI GCCGGC 1 cut(s) 363
Kzo9I GATC 3 cut(s) 286, 366, 510
LguI GCTCTTC 1 cut(s) 506
LmnI GCTCC 5 cut(s) 29, 94, 215, 435, 893
Lsp1109I GCAGC 1 cut(s) 664
LweI GCATC 2 cut(s) 405, 691
MaeII ACGT 2 cut(s) 537, 758
MalI GATC 3 cut(s) 288, 368, 512
MboI GATC 3 cut(s) 286, 366, 510
MhlI GDGCHC 4 cut(s) 432, 501, 663, 879
MluCI AATT 1 cut(s) 247
MmeI TCCRAC 1 cut(s) 288
MroNI GCCGGC 1 cut(s) 361
MslI CAYNNNNRTG 1 cut(s) 614
MspI CCGG 3 cut(s) 362, 579, 753
MspR9I CCNGG 1 cut(s) 754
Mva1269I GAATGC 1 cut(s) 744
MwoI GCNNNNNNNGC 7 cut(s) 207, 551, 572, 641, 677, 683, 785
NaeI GCCGGC 1 cut(s) 363
NciI CCSGG 1 cut(s) 754
NdeII GATC 3 cut(s) 286, 366, 510
NgoMIV GCCGGC 1 cut(s) 361
NlaIII CATG 2 cut(s) 532, 613
NlaIV GGNNCC 1 cut(s) 431
NmeAIII GCCGAG 1 cut(s) 814
NsbI TGCGCA 1 cut(s) 419
NspI RCATGY 2 cut(s) 532, 613
PceI AGGCCT 2 cut(s) 491, 566
PciSI GCTCTTC 1 cut(s) 506
PcsI WCGNNNNNNNCGW 6 cut(s) 273, 276, 282, 314, 320, 690
PctI GAATGC 1 cut(s) 744
PdiI GCCGGC 1 cut(s) 363
PkrI GCNGC 6 cut(s) 7, 574, 577, 679, 744, 787
Ple19I CGATCG 1 cut(s) 369
Psp124BI GAGCTC 1 cut(s) 501
PspN4I GGNNCC 1 cut(s) 431
PspPI GGNCC 3 cut(s) 523, 544, 744
PvuI CGATCG 1 cut(s) 369
RsaI GTAC 1 cut(s) 608
RsaNI GTAC 1 cut(s) 607
RseI CAYNNNNRTG 1 cut(s) 614
SacI GAGCTC 1 cut(s) 501
SapI GCTCTTC 1 cut(s) 506
SatI GCNGC 6 cut(s) 6, 573, 576, 678, 743, 786
Sau3AI GATC 3 cut(s) 286, 366, 510
Sau96I GGNCC 3 cut(s) 523, 544, 744
ScrFI CCNGG 1 cut(s) 754
SduI GDGCHC 4 cut(s) 432, 501, 663, 879
SfaNI GCATC 2 cut(s) 405, 691
SmiMI CAYNNNNRTG 1 cut(s) 614
SmlI CTYRAG 2 cut(s) 599, 878
SmoI CTYRAG 2 cut(s) 599, 878
Sse9I AATT 1 cut(s) 247
SseBI AGGCCT 2 cut(s) 491, 566
SstI GAGCTC 1 cut(s) 501
StuI AGGCCT 2 cut(s) 491, 566
StyD4I CCNGG 1 cut(s) 752
StyI CCWWGG 2 cut(s) 468, 588
TaiI ACGT 2 cut(s) 540, 761
TaqI TCGA 3 cut(s) 369, 456, 561
TasI AATT 1 cut(s) 247
TatI WGTACW 1 cut(s) 606
TauI GCSGC 5 cut(s) 8, 575, 578, 745, 788
TseI GCWGC 1 cut(s) 677
TspDTI ATGAA 2 cut(s) 129, 845
XceI RCATGY 2 cut(s) 532, 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.