Rmu_sc0002745.1_g000012

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002745.1
OG0017798
Physical Location & Seq
Reverse (-)
49910 .. 51501
1592 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002745.1_g000012.1.cds

Sequence Viewer

Length: 549 bp
atgaaggatacagccgccaactgcgctgccgtatcttctccactacagcgtaggaggaccatgtctgctctcaaagcccgaagctccctcgtcgccgctctcgccacccaagcctccttcgtctcccaggaacataaatttttactcgatttggcgaagtacgtcttccctaagcagttcgaggctcggactctcgaggaagctctcatgtcagggtctcctcctttgagttctagtggtagtagtggtggcggtggcggccctgtttctctggatatccggcgggctgcagagagtgtggggttcttttatgtggttaaccatgggatacccaagacagtgttggaggagatgcttgaggtgacgcgtgggtttcatgagctgccaagggaggtgaaggctgagtactatactagggatcccaagaggaaggtcaaatactttagtagctttaatttgtacgagtcaaagtttgctgcttggagggacagcttgttttgtgacatggctccggagcccttggatccccaagaattgcctctggtttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

182

Amino Acids

20.16

Weight (kDa)

8.52

Isoelectric Point (pI)

62.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 98
AccII CGCG 1 cut(s) 367
AccIII TCCGGA 1 cut(s) 511
AciI CCGC 5 cut(s) 15, 96, 252, 258, 283
AclWI GGATC 4 cut(s) 413, 426, 518, 531
AcsI RAATTY 1 cut(s) 137
AfaI GTAC 3 cut(s) 161, 407, 461
AflIII ACRYGT 1 cut(s) 365
AjnI CCWGG 1 cut(s) 126
AluBI AGCT 5 cut(s) 84, 203, 382, 450, 492
AluI AGCT 5 cut(s) 84, 203, 382, 450, 492
Alw26I GTCTC 2 cut(s) 127, 222
AlwI GGATC 4 cut(s) 413, 426, 518, 531
Ama87I CYCGRG 1 cut(s) 194
Aor13HI TCCGGA 1 cut(s) 511
AoxI GGCC 1 cut(s) 259
ApeKI GCWGC 4 cut(s) 26, 287, 382, 476
ApoI RAATTY 1 cut(s) 137
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 2 cut(s) 57, 260
AsuHPI GGTGA 2 cut(s) 373, 406
AvaI CYCGRG 1 cut(s) 194
AvaII GGWCC 1 cut(s) 57
BamHI GGATCC 2 cut(s) 418, 523
BanII GRGCYC 1 cut(s) 519
BarI GAAGNNNNNNTAC 4 cut(s) 389, 421, 422, 454
BbsI GAAGAC 1 cut(s) 157
BbvI GCAGC 4 cut(s) 13, 274, 369, 463
BceAI ACGGC 1 cut(s) 14
BciT130I CCWGG 1 cut(s) 128
BciVI GTATCC 1 cut(s) 321
BcoDI GTCTC 2 cut(s) 127, 222
BfaI CTAG 2 cut(s) 234, 414
BfmI CTRYAG 2 cut(s) 44, 288
BfuI GTATCC 1 cut(s) 321
BisI GCNGC 7 cut(s) 15, 27, 96, 259, 288, 383, 477
BlsI GCNGC 7 cut(s) 16, 28, 97, 260, 289, 384, 478
BmcAI AGTACT 1 cut(s) 407
Bme1390I CCNGG 1 cut(s) 128
Bme18I GGWCC 1 cut(s) 57
BmeT110I CYCGRG 1 cut(s) 194
BmgT120I GGNCC 2 cut(s) 57, 260
BmiI GGNNCC 4 cut(s) 420, 510, 516, 525
BmrFI CCNGG 1 cut(s) 128
BmsI GCATC 1 cut(s) 342
BpiI GAAGAC 1 cut(s) 157
Bpu10I CCTNAGC 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 377
BsaI GGTCTC 1 cut(s) 222
BsaJI CCNNGG 4 cut(s) 126, 322, 386, 519
BsaWI WCCGGW 1 cut(s) 511
BseAI TCCGGA 1 cut(s) 511
BseBI CCWGG 1 cut(s) 128
BseDI CCNNGG 4 cut(s) 126, 322, 386, 519
BseMII CTCAG 1 cut(s) 393
BseRI GAGGAG 2 cut(s) 210, 362
BseXI GCAGC 4 cut(s) 13, 274, 369, 463
Bsh1236I CGCG 1 cut(s) 367
BshFI GGCC 1 cut(s) 261
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 2 cut(s) 280, 512
BslFI GGGAC 1 cut(s) 500
BsmAI GTCTC 2 cut(s) 127, 222
BsmBI CGTCTC 1 cut(s) 127
BsmFI GGGAC 1 cut(s) 500
BsnI GGCC 1 cut(s) 261
Bso31I GGTCTC 1 cut(s) 222
BsoBI CYCGRG 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 519
Bsp13I TCCGGA 1 cut(s) 511
Bsp143I GATC 2 cut(s) 418, 523
Bsp19I CCATGG 1 cut(s) 322
BspACI CCGC 5 cut(s) 15, 96, 252, 258, 283
BspANI GGCC 1 cut(s) 261
BspCNI CTCAG 1 cut(s) 394
BspEI TCCGGA 1 cut(s) 511
BspFNI CGCG 1 cut(s) 367
BspHI TCATGA 1 cut(s) 376
BspLI GGNNCC 4 cut(s) 420, 510, 516, 525
BspMAI CTGCAG 1 cut(s) 292
BspPI GGATC 4 cut(s) 413, 426, 518, 531
BspTNI GGTCTC 1 cut(s) 222
BsrBI CCGCTC 1 cut(s) 98
BssECI CCNNGG 4 cut(s) 126, 322, 386, 519
BssMI GATC 2 cut(s) 418, 523
BssT1I CCWWGG 3 cut(s) 322, 386, 519
Bst2UI CCWGG 1 cut(s) 128
Bst4CI ACNGT 1 cut(s) 340
BstC8I GCNNGC 1 cut(s) 285
BstDEI CTNAG 2 cut(s) 171, 402
BstDSI CCRYGG 1 cut(s) 322
BstFNI CGCG 1 cut(s) 367
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 2 cut(s) 421, 526
BstMAI GTCTC 2 cut(s) 127, 222
BstMBI GATC 2 cut(s) 418, 523
BstMWI GCNNNNNNNGC 5 cut(s) 23, 74, 101, 110, 258
BstNI CCWGG 1 cut(s) 128
BstSCI CCNGG 1 cut(s) 126
BstSFI CTRYAG 2 cut(s) 44, 288
BstUI CGCG 1 cut(s) 367
BstV1I GCAGC 4 cut(s) 13, 274, 369, 463
BstV2I GAAGAC 1 cut(s) 157
BstX2I RGATCY 2 cut(s) 418, 523
BstYI RGATCY 2 cut(s) 418, 523
BsuI GTATCC 1 cut(s) 321
BsuRI GGCC 1 cut(s) 261
BtgI CCRYGG 1 cut(s) 322
BtsIMutI CAGTG 1 cut(s) 345
Cac8I GCNNGC 1 cut(s) 285
CciI TCATGA 1 cut(s) 376
CfoI GCGC 1 cut(s) 26
Cfr13I GGNCC 2 cut(s) 57, 260
CseI GACGC 1 cut(s) 373
Csp6I GTAC 3 cut(s) 160, 406, 460
CviAII CATG 5 cut(s) 61, 208, 323, 377, 505
CviQI GTAC 3 cut(s) 160, 406, 460
DdeI CTNAG 2 cut(s) 171, 402
DpnI GATC 2 cut(s) 420, 525
DpnII GATC 2 cut(s) 418, 523
Eco130I CCWWGG 3 cut(s) 322, 386, 519
Eco24I GRGCYC 1 cut(s) 519
Eco31I GGTCTC 1 cut(s) 222
Eco32I GATATC 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 57
Eco88I CYCGRG 1 cut(s) 194
EcoRII CCWGG 1 cut(s) 126
EcoRV GATATC 1 cut(s) 277
EcoT14I CCWWGG 3 cut(s) 322, 386, 519
EcoT38I GRGCYC 1 cut(s) 519
ErhI CCWWGG 3 cut(s) 322, 386, 519
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 5 cut(s) 64, 211, 326, 380, 508
FaiI YATR 8 cut(s) 62, 135, 209, 312, 324, 378, 411, 506
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FaqI GGGAC 1 cut(s) 500
FatI CATG 5 cut(s) 60, 207, 322, 376, 504
FauI CCCGC 1 cut(s) 276
Fnu4HI GCNGC 7 cut(s) 15, 27, 96, 259, 288, 383, 477
FriOI GRGCYC 1 cut(s) 519
Fsp4HI GCNGC 7 cut(s) 15, 27, 96, 259, 288, 383, 477
FspBI CTAG 2 cut(s) 234, 414
GlaI GCGC 1 cut(s) 25
GluI GCNGC 7 cut(s) 15, 27, 96, 259, 288, 383, 477
HaeIII GGCC 1 cut(s) 261
HapII CCGG 2 cut(s) 280, 512
HgaI GACGC 1 cut(s) 373
HhaI GCGC 1 cut(s) 26
Hin1II CATG 5 cut(s) 64, 211, 326, 380, 508
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HinfI GANTC 2 cut(s) 190, 464
HpaI GTTAAC 1 cut(s) 319
HpaII CCGG 2 cut(s) 280, 512
HphI GGTGA 2 cut(s) 373, 406
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 1 cut(s) 189
Hpy188III TCNNGA 4 cut(s) 194, 272, 377, 512
Hpy8I GTNNAC 1 cut(s) 319
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 3 cut(s) 127, 391, 424
HpyCH4III ACNGT 1 cut(s) 340
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 1 cut(s) 290
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 74, 101, 110, 258
HpyF3I CTNAG 2 cut(s) 171, 402
HpySE526I ACGT 1 cut(s) 162
Hsp92II CATG 5 cut(s) 64, 211, 326, 380, 508
HspAI GCGC 1 cut(s) 24
Kpn2I TCCGGA 1 cut(s) 511
KspAI GTTAAC 1 cut(s) 319
Kzo9I GATC 2 cut(s) 418, 523
LmnI GCTCC 3 cut(s) 89, 514, 514
LpnPI CCDG 8 cut(s) 113, 140, 198, 257, 276, 293, 525, 527
Lsp1109I GCAGC 4 cut(s) 13, 274, 369, 463
LweI GCATC 1 cut(s) 342
MaeI CTAG 2 cut(s) 234, 414
MaeII ACGT 1 cut(s) 162
MaeIII GTNAC 2 cut(s) 361, 500
MalI GATC 2 cut(s) 420, 525
MbiI CCGCTC 1 cut(s) 98
MboI GATC 2 cut(s) 418, 523
MboII GAAGA 2 cut(s) 27, 157
MflI RGATCY 2 cut(s) 418, 523
MhlI GDGCHC 1 cut(s) 519
MluCI AATT 3 cut(s) 137, 454, 533
MluI ACGCGT 1 cut(s) 365
MlyI GAGTC 2 cut(s) 184, 473
MmeI TCCRAC 1 cut(s) 324
MroI TCCGGA 1 cut(s) 511
MseI TTAA 2 cut(s) 318, 453
MspI CCGG 2 cut(s) 280, 512
MspR9I CCNGG 1 cut(s) 128
MvaI CCWGG 1 cut(s) 128
MvnI CGCG 1 cut(s) 367
MwoI GCNNNNNNNGC 5 cut(s) 23, 74, 101, 110, 258
NcoI CCATGG 1 cut(s) 322
NdeII GATC 2 cut(s) 418, 523
NlaIII CATG 5 cut(s) 64, 211, 326, 380, 508
NlaIV GGNNCC 4 cut(s) 420, 510, 516, 525
NmuCI GTSAC 2 cut(s) 361, 500
PaeR7I CTCGAG 1 cut(s) 194
PagI TCATGA 1 cut(s) 376
PflFI GACNNNGTC 1 cut(s) 61
PkrI GCNGC 7 cut(s) 16, 28, 97, 260, 289, 384, 478
PleI GAGTC 2 cut(s) 184, 472
PpsI GAGTC 2 cut(s) 184, 472
Psp6I CCWGG 1 cut(s) 126
PspGI CCWGG 1 cut(s) 126
PspN4I GGNNCC 4 cut(s) 420, 510, 516, 525
PspPI GGNCC 2 cut(s) 57, 260
PstI CTGCAG 1 cut(s) 292
PsuI RGATCY 2 cut(s) 418, 523
PsyI GACNNNGTC 1 cut(s) 61
RsaI GTAC 3 cut(s) 161, 407, 461
RsaNI GTAC 3 cut(s) 160, 406, 460
SaqAI TTAA 2 cut(s) 318, 453
SatI GCNGC 7 cut(s) 15, 27, 96, 259, 288, 383, 477
Sau3AI GATC 2 cut(s) 418, 523
Sau96I GGNCC 2 cut(s) 57, 260
ScaI AGTACT 1 cut(s) 407
SchI GAGTC 2 cut(s) 184, 473
ScrFI CCNGG 1 cut(s) 128
SduI GDGCHC 1 cut(s) 519
SetI ASST 9 cut(s) 86, 165, 205, 363, 384, 396, 435, 452, 494
SfaNI GCATC 1 cut(s) 342
SfcI CTRYAG 2 cut(s) 44, 288
Sfr274I CTCGAG 1 cut(s) 194
SinI GGWCC 1 cut(s) 57
SlaI CTCGAG 1 cut(s) 194
SmlI CTYRAG 2 cut(s) 194, 356
SmoI CTYRAG 2 cut(s) 194, 356
Sse9I AATT 3 cut(s) 137, 454, 533
SsiI CCGC 5 cut(s) 15, 96, 252, 258, 283
SspMI CTAG 2 cut(s) 234, 414
StyD4I CCNGG 1 cut(s) 126
StyI CCWWGG 3 cut(s) 322, 386, 519
TaaI ACNGT 1 cut(s) 340
TaiI ACGT 1 cut(s) 165
TaqI TCGA 3 cut(s) 147, 180, 195
TasI AATT 3 cut(s) 137, 454, 533
TatI WGTACW 1 cut(s) 405
TauI GCSGC 3 cut(s) 17, 98, 261
Tru1I TTAA 2 cut(s) 318, 453
Tru9I TTAA 2 cut(s) 318, 453
TscAI CASTG 1 cut(s) 345
TseFI GTSAC 2 cut(s) 361, 500
TseI GCWGC 4 cut(s) 26, 287, 382, 476
Tsp45I GTSAC 2 cut(s) 361, 500
TspDTI ATGAA 2 cut(s) 17, 365
TspRI CASTG 1 cut(s) 345
Tth111I GACNNNGTC 1 cut(s) 61
VpaK11BI GGWCC 1 cut(s) 57
XapI RAATTY 1 cut(s) 137
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XhoI CTCGAG 1 cut(s) 194
XspI CTAG 2 cut(s) 234, 414
ZrmI AGTACT 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.