Rh5BG244300

Heme-binding-like protein At3g10130

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0017798
Physical Location & Seq
Forward (+)
29564742 .. 29573886
9145 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG244300.1

Sequence Viewer

Length: 288 bp
ATGAAGGATACGGCCGCCAACCGCGCTGCCGTATCTTCTCCACTGCAGCGAAGGAGGACCATGTCTGCTCTCAAAGCCCGAAGCTCCCTCGTCGCTGCTCTTGCCACCCAAGCCTCCTCCGTCTCCCAGGAACATAAATTTTTACTTGATTTGGCCAAGTACGTCTTCCCCAAGCAGTTCGAGGCTCAGACTCTCGAGGAAGCTCTCATGTCAGGTATATGGATTGAAGGGTCTAAGACCGAGATACCCAAGAGCCTGGAAAGAAATAAGGAGATTGGGACATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

95

Amino Acids

10.51

Weight (kDa)

9.69

Isoelectric Point (pI)

73.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 24
AciI CCGC 2 cut(s) 15, 22
AcoI YGGCCR 2 cut(s) 12, 153
AcsI RAATTY 1 cut(s) 137
AfaI GTAC 1 cut(s) 161
AgsI TTSAA 1 cut(s) 227
AjnI CCWGG 2 cut(s) 126, 255
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AluBI AGCT 2 cut(s) 84, 203
AluI AGCT 2 cut(s) 84, 203
Alw26I GTCTC 1 cut(s) 127
Ama87I CYCGRG 1 cut(s) 194
AoxI GGCC 2 cut(s) 12, 153
ApeKI GCWGC 3 cut(s) 26, 46, 95
ApoI RAATTY 1 cut(s) 137
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 1 cut(s) 57
AvaI CYCGRG 1 cut(s) 194
AvaII GGWCC 1 cut(s) 57
BalI TGGCCA 1 cut(s) 155
BbsI GAAGAC 1 cut(s) 157
BbvI GCAGC 3 cut(s) 13, 58, 82
BceAI ACGGC 2 cut(s) 14, 27
BciT130I CCWGG 2 cut(s) 128, 257
BcoDI GTCTC 1 cut(s) 127
BfmI CTRYAG 1 cut(s) 44
BisI GCNGC 4 cut(s) 15, 27, 47, 96
BlsI GCNGC 4 cut(s) 16, 28, 48, 97
Bme1390I CCNGG 2 cut(s) 128, 257
Bme18I GGWCC 1 cut(s) 57
BmeT110I CYCGRG 1 cut(s) 194
BmgT120I GGNCC 1 cut(s) 57
BmrFI CCNGG 2 cut(s) 128, 257
BpiI GAAGAC 1 cut(s) 157
BsaJI CCNNGG 1 cut(s) 126
BseBI CCWGG 2 cut(s) 128, 257
BseDI CCNNGG 1 cut(s) 126
BseMII CTCAG 1 cut(s) 200
BseRI GAGGAG 1 cut(s) 106
BseX3I CGGCCG 1 cut(s) 12
BseXI GCAGC 3 cut(s) 13, 58, 82
Bsh1236I CGCG 1 cut(s) 24
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 2 cut(s) 14, 155
BsiEI CGRYCG 1 cut(s) 15
BsiHKCI CYCGRG 1 cut(s) 194
BsmAI GTCTC 1 cut(s) 127
BsmBI CGTCTC 1 cut(s) 127
BsnI GGCC 2 cut(s) 14, 155
BsoBI CYCGRG 1 cut(s) 194
BspACI CCGC 2 cut(s) 15, 22
BspANI GGCC 2 cut(s) 14, 155
BspCNI CTCAG 1 cut(s) 199
BspFNI CGCG 1 cut(s) 24
BspMAI CTGCAG 1 cut(s) 48
BssECI CCNNGG 1 cut(s) 126
Bst2UI CCWGG 2 cut(s) 128, 257
BstDEI CTNAG 2 cut(s) 186, 234
BstFNI CGCG 1 cut(s) 24
BstHHI GCGC 1 cut(s) 26
BstMAI GTCTC 1 cut(s) 127
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 4 cut(s) 23, 74, 101, 110
BstNI CCWGG 2 cut(s) 128, 257
BstSCI CCNGG 2 cut(s) 126, 255
BstSFI CTRYAG 1 cut(s) 44
BstUI CGCG 1 cut(s) 24
BstV1I GCAGC 3 cut(s) 13, 58, 82
BstV2I GAAGAC 1 cut(s) 157
BstXI CCANNNNNNTGG 1 cut(s) 256
BstZI CGGCCG 1 cut(s) 12
BsuRI GGCC 2 cut(s) 14, 155
BtsI GCAGTG 1 cut(s) 41
BtsIMutI CAGTG 1 cut(s) 41
CfoI GCGC 1 cut(s) 26
Cfr13I GGNCC 1 cut(s) 57
Csp6I GTAC 1 cut(s) 160
CviAII CATG 2 cut(s) 61, 208
CviJI RGCY 8 cut(s) 14, 77, 84, 113, 155, 185, 203, 255
CviKI_1 RGCY 8 cut(s) 14, 77, 84, 113, 155, 185, 203, 255
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 2 cut(s) 186, 234
EaeI YGGCCR 2 cut(s) 12, 153
EagI CGGCCG 1 cut(s) 12
EclXI CGGCCG 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 57
Eco52I CGGCCG 1 cut(s) 12
Eco88I CYCGRG 1 cut(s) 194
EcoRII CCWGG 2 cut(s) 126, 255
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 2 cut(s) 64, 211
FaiI YATR 5 cut(s) 62, 135, 209, 218, 220
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FatI CATG 2 cut(s) 60, 207
Fnu4HI GCNGC 4 cut(s) 15, 27, 47, 96
Fsp4HI GCNGC 4 cut(s) 15, 27, 47, 96
GlaI GCGC 1 cut(s) 25
GluI GCNGC 4 cut(s) 15, 27, 47, 96
HaeIII GGCC 2 cut(s) 14, 155
HhaI GCGC 1 cut(s) 26
Hin1II CATG 2 cut(s) 64, 211
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HinfI GANTC 1 cut(s) 190
Hpy188I TCNGA 1 cut(s) 189
Hpy188III TCNNGA 1 cut(s) 194
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 2 cut(s) 45, 221
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 1 cut(s) 46
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 74, 101, 110
HpyF3I CTNAG 2 cut(s) 186, 234
HpySE526I ACGT 1 cut(s) 162
Hsp92II CATG 2 cut(s) 64, 211
HspAI GCGC 1 cut(s) 24
LmnI GCTCC 1 cut(s) 89
LpnPI CCDG 5 cut(s) 113, 140, 198, 242, 269
Lsp1109I GCAGC 3 cut(s) 13, 58, 82
MaeII ACGT 1 cut(s) 162
MboII GAAGA 2 cut(s) 27, 157
MlsI TGGCCA 1 cut(s) 155
MluCI AATT 1 cut(s) 137
MluNI TGGCCA 1 cut(s) 155
MlyI GAGTC 1 cut(s) 184
MnlI CCTC 6 cut(s) 48, 98, 124, 127, 175, 190
Mox20I TGGCCA 1 cut(s) 155
MscI TGGCCA 1 cut(s) 155
Msp20I TGGCCA 1 cut(s) 155
MspR9I CCNGG 2 cut(s) 128, 257
MvaI CCWGG 2 cut(s) 128, 257
MvnI CGCG 1 cut(s) 24
MwoI GCNNNNNNNGC 4 cut(s) 23, 74, 101, 110
NlaIII CATG 2 cut(s) 64, 211
PaeR7I CTCGAG 1 cut(s) 194
PflFI GACNNNGTC 1 cut(s) 61
PkrI GCNGC 4 cut(s) 16, 28, 48, 97
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
Psp6I CCWGG 2 cut(s) 126, 255
PspGI CCWGG 2 cut(s) 126, 255
PspPI GGNCC 1 cut(s) 57
PstI CTGCAG 1 cut(s) 48
PsyI GACNNNGTC 1 cut(s) 61
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
SatI GCNGC 4 cut(s) 15, 27, 47, 96
Sau96I GGNCC 1 cut(s) 57
SchI GAGTC 1 cut(s) 184
ScrFI CCNGG 2 cut(s) 128, 257
SetI ASST 4 cut(s) 86, 165, 205, 217
SfcI CTRYAG 1 cut(s) 44
Sfr274I CTCGAG 1 cut(s) 194
SinI GGWCC 1 cut(s) 57
SlaI CTCGAG 1 cut(s) 194
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
Sse9I AATT 1 cut(s) 137
SsiI CCGC 2 cut(s) 15, 22
StyD4I CCNGG 2 cut(s) 126, 255
TaiI ACGT 1 cut(s) 165
TaqI TCGA 2 cut(s) 180, 195
TaqII GACCGA 1 cut(s) 254
TasI AATT 1 cut(s) 137
TauI GCSGC 1 cut(s) 17
TscAI CASTG 1 cut(s) 48
TseI GCWGC 3 cut(s) 26, 46, 95
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 109
TspRI CASTG 1 cut(s) 48
Tth111I GACNNNGTC 1 cut(s) 61
VpaK11BI GGWCC 1 cut(s) 57
XapI RAATTY 1 cut(s) 137
XhoI CTCGAG 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.