Rmu_sc0002890.1_g000010

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002890.1
OG0005471
Physical Location & Seq
Reverse (-)
47364 .. 48755
1392 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1242 bp
atggttttcagacccgagtgcaaaccaattcagactcggttgcattttccaatttgcatgatggatgctgtagttccgcatcaagttgagcatgacttttcagatgtatttggtcctgtaccagtccagaccccagtggattcagctaattctgcctctctccaagatagtacggatcccgtattcattcacaaccggtcacactttttgattggtccttcattctatgtaagccaatcactgaacctcaacaaactgactatttataacacagaagattcagtggacctagcagagtactgtctccatgaagaaaccatcaaagaatctcaggaaccttttgttggtgaccccgtcattgggcatcctctagaaaatgttgaagagagtttcttgaagagtgagagtgtaggcatcgaagattttgaggttttgaagattgttgggcagggtggatttgcaaaagtatatcaggtgaggaagaagggaacctcagaaatatacgcgatgaaggtaatgcgaaaggacaagatcatgaagaataatcttgctgaatacatgaaatttgagagggatatattaacaaaagttgatcatccattcattgtcaagcttagatactcattccaaaccaaatacagactatatcttgtgctggattttgtcaacggagccgagattgtttctgcggtgtctcaccttcattcaaatggcataatgcatagagatcttaagcccgaaaatatcctattggatgcagatggccatgccattttaactgattttgggcttgcgaagagctttgacgaaaacacgaggtctaattctctatgtggaactgtagaatacatggcaccggaaatagttcagggaaatggccatgataaggcagcagattggtggagtgtgggagttgtattgtttcagatgcttactggcaagcttccttttactgggaaccgcttgttagcgaaggacacaagcaagcgcctaggttgtggacctctgggaggtgatgaagtcaagcagcacaaatggttcaagcaaattaattggaagaaattggatgctcgtgaaatcaaaccgagcttccgacctaacgttcccgggcagcactgcattgccaacattgagaattactggactgacatgccacttgtggactccccggctgctagtccgaaggcttcgtctggaaatcccttctccggctttagttatgttaggtctgcagcctcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008285 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009651 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0035556 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045727 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04350 ko04371 ko04666 ko04714 ko04910 ko04931 ko05165 ko05200 ko05205 ko05210 ko05212 ko05221 ko05224 ko05225 ko05226 ko05231 map01521 map01522 map04012 map04066 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04350 map04371 map04666 map04714 map04910 map04931 map05165 map05200 map05205 map05210 map05212 map05221 map05224 map05225 map05226 map05231
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

413

Amino Acids

46.4

Weight (kDa)

6.11

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 142 - 320 1e-53 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 144 - 226 9.7e-06 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 226 - 319 2.9e-18 Protein tyrosine and serine/threonine kinase
Pkinase_fungal PF17667 227 - 307 4.1e-08 Fungal protein kinase
Kinase-like PF14531 227 - 323 6.1e-07 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 267
AccB1I GGYRCC 1 cut(s) 853
AccII CGCG 1 cut(s) 506
AciI CCGC 3 cut(s) 77, 689, 961
AclI AACGTT 1 cut(s) 1101
AclWI GGATC 2 cut(s) 170, 183
AcoI YGGCCR 2 cut(s) 763, 877
AcsI RAATTY 1 cut(s) 563
AfaI GTAC 3 cut(s) 120, 172, 299
AfiI CCNNNNNNNGG 7 cut(s) 344, 359, 360, 886, 953, 1010, 1208
AflII CTTAAG 1 cut(s) 731
AgeI ACCGGT 1 cut(s) 195
AgsI TTSAA 5 cut(s) 383, 397, 436, 708, 1042
AjuI GAANNNNNNNTTGG 2 cut(s) 327, 359
AluBI AGCT 5 cut(s) 146, 613, 801, 943, 1089
AluI AGCT 5 cut(s) 146, 613, 801, 943, 1089
Alw26I GTCTC 2 cut(s) 308, 699
AlwI GGATC 2 cut(s) 170, 183
Ama87I CYCGRG 2 cut(s) 14, 1106
AoxI GGCC 2 cut(s) 763, 877
ApeKI GCWGC 5 cut(s) 890, 1027, 1111, 1172, 1232
ApoI RAATTY 1 cut(s) 563
ArsI GACNNNNNNTTYG 2 cut(s) 1175, 1207
AseI ATTAAT 1 cut(s) 1050
AsiGI ACCGGT 1 cut(s) 195
Asp700I GAANNNNTTC 1 cut(s) 864
AspA2I CCTAGG 1 cut(s) 991
AspLEI GCGC 1 cut(s) 990
AspS9I GGNCC 4 cut(s) 113, 215, 286, 1001
AsuC2I CCSGG 3 cut(s) 1107, 1108, 1169
AsuHPI GGTGA 4 cut(s) 359, 487, 689, 1025
AvaI CYCGRG 2 cut(s) 14, 1106
AvaII GGWCC 4 cut(s) 113, 215, 286, 1001
AvrII CCTAGG 1 cut(s) 991
BalI TGGCCA 2 cut(s) 765, 879
BamHI GGATCC 1 cut(s) 175
BanI GGYRCC 1 cut(s) 853
BauI CACGAG 2 cut(s) 814, 1071
BbvI GCAGC 4 cut(s) 902, 1039, 1123, 1159
BccI CCATC 3 cut(s) 55, 326, 755
BclI TGATCA 1 cut(s) 592
BcnI CCSGG 3 cut(s) 1107, 1108, 1169
BcoDI GTCTC 2 cut(s) 308, 699
BfaI CTAG 4 cut(s) 290, 371, 992, 1176
BfmI CTRYAG 3 cut(s) 69, 840, 1230
BfoI RGCGCY 1 cut(s) 991
BfrI CTTAAG 1 cut(s) 731
BglII AGATCT 1 cut(s) 727
BisI GCNGC 5 cut(s) 891, 1028, 1112, 1173, 1233
BlnI CCTAGG 1 cut(s) 991
BlsI GCNGC 5 cut(s) 892, 1029, 1113, 1174, 1234
BmcAI AGTACT 1 cut(s) 299
Bme1390I CCNGG 3 cut(s) 1107, 1108, 1169
Bme18I GGWCC 4 cut(s) 113, 215, 286, 1001
BmeT110I CYCGRG 2 cut(s) 14, 1106
BmgT120I GGNCC 4 cut(s) 113, 215, 286, 1001
BmiI GGNNCC 6 cut(s) 177, 336, 490, 673, 855, 959
BmrFI CCNGG 3 cut(s) 1107, 1108, 1169
BmrI ACTGGG 2 cut(s) 128, 963
BmsI GCATC 7 cut(s) 55, 88, 373, 423, 745, 918, 1057
BmuI ACTGGG 2 cut(s) 128, 963
BpuMI CCSGG 3 cut(s) 1107, 1108, 1169
BsaJI CCNNGG 3 cut(s) 991, 1106, 1167
BsaWI WCCGGW 2 cut(s) 195, 856
Bsc4I CCNNNNNNNGG 7 cut(s) 344, 359, 360, 886, 953, 1010, 1208
Bse118I RCCGGY 1 cut(s) 195
Bse1I ACTGG 5 cut(s) 122, 134, 940, 958, 1145
Bse3DI GCAATG 1 cut(s) 1119
BseDI CCNNGG 3 cut(s) 991, 1106, 1167
BseGI GGATG 5 cut(s) 70, 364, 595, 760, 1072
BseLI CCNNNNNNNGG 7 cut(s) 344, 359, 360, 886, 953, 1010, 1208
BseMI GCAATG 1 cut(s) 1119
BseMII CTCAG 2 cut(s) 344, 507
BseNI ACTGG 5 cut(s) 122, 134, 940, 958, 1145
BseXI GCAGC 4 cut(s) 902, 1039, 1123, 1159
Bsh1236I CGCG 1 cut(s) 506
BshFI GGCC 2 cut(s) 765, 879
BshNI GGYRCC 1 cut(s) 853
BshTI ACCGGT 1 cut(s) 195
BsiHKCI CYCGRG 2 cut(s) 14, 1106
BsiSI CCGG 5 cut(s) 196, 857, 1107, 1169, 1209
BslI CCNNNNNNNGG 7 cut(s) 344, 359, 360, 886, 953, 1010, 1208
BsmAI GTCTC 2 cut(s) 308, 699
BsnI GGCC 2 cut(s) 765, 879
BsoBI CYCGRG 2 cut(s) 14, 1106
Bsp143I GATC 4 cut(s) 175, 531, 592, 727
BspACI CCGC 3 cut(s) 77, 689, 961
BspANI GGCC 2 cut(s) 765, 879
BspCNI CTCAG 2 cut(s) 343, 506
BspFNI CGCG 1 cut(s) 506
BspHI TCATGA 1 cut(s) 534
BspLI GGNNCC 6 cut(s) 177, 336, 490, 673, 855, 959
BspMAI CTGCAG 1 cut(s) 1234
BspPI GGATC 2 cut(s) 170, 183
BspQI GCTCTTC 1 cut(s) 791
BspT107I GGYRCC 1 cut(s) 853
BspTI CTTAAG 1 cut(s) 731
BsrDI GCAATG 1 cut(s) 1119
BsrFI RCCGGY 1 cut(s) 195
BsrI ACTGG 5 cut(s) 122, 134, 940, 958, 1145
BssAI RCCGGY 1 cut(s) 195
BssECI CCNNGG 3 cut(s) 991, 1106, 1167
BssMI GATC 4 cut(s) 175, 531, 592, 727
BssSI CACGAG 2 cut(s) 814, 1071
BssT1I CCWWGG 1 cut(s) 991
Bst2BI CACGAG 2 cut(s) 814, 1071
Bst4CI ACNGT 2 cut(s) 302, 841
Bst6I CTCTTC 3 cut(s) 378, 392, 791
BstAFI CTTAAG 1 cut(s) 731
BstC8I GCNNGC 3 cut(s) 792, 941, 986
BstDEI CTNAG 3 cut(s) 330, 493, 614
BstEII GGTNACC 1 cut(s) 347
BstENI CCTNNNNNAGG 1 cut(s) 1008
BstF5I GGATG 5 cut(s) 70, 364, 595, 760, 1072
BstFNI CGCG 1 cut(s) 506
BstH2I RGCGCY 1 cut(s) 991
BstHHI GCGC 1 cut(s) 990
BstKTI GATC 4 cut(s) 178, 534, 595, 730
BstMAI GTCTC 2 cut(s) 308, 699
BstMBI GATC 4 cut(s) 175, 531, 592, 727
BstMWI GCNNNNNNNGC 1 cut(s) 152
BstNSI RCATGY 1 cut(s) 1153
BstPI GGTNACC 1 cut(s) 347
BstSCI CCNGG 3 cut(s) 1105, 1106, 1167
BstSFI CTRYAG 3 cut(s) 69, 840, 1230
BstUI CGCG 1 cut(s) 506
BstV1I GCAGC 4 cut(s) 902, 1039, 1123, 1159
BstX2I RGATCY 2 cut(s) 175, 727
BstYI RGATCY 2 cut(s) 175, 727
BsuRI GGCC 2 cut(s) 765, 879
BtgZI GCGATG 1 cut(s) 521
BtsCI GGATG 5 cut(s) 70, 364, 595, 760, 1072
BtsI GCAGTG 1 cut(s) 1114
BtsIMutI CAGTG 4 cut(s) 141, 239, 288, 1114
Cac8I GCNNGC 3 cut(s) 792, 941, 986
CciI TCATGA 1 cut(s) 534
CfoI GCGC 1 cut(s) 990
Cfr10I RCCGGY 1 cut(s) 195
Cfr13I GGNCC 4 cut(s) 113, 215, 286, 1001
Cfr9I CCCGGG 1 cut(s) 1106
Csp6I GTAC 3 cut(s) 119, 171, 298
CspAI ACCGGT 1 cut(s) 195
CviAII CATG 9 cut(s) 58, 92, 308, 535, 559, 767, 850, 881, 1150
CviQI GTAC 3 cut(s) 119, 171, 298
DdeI CTNAG 3 cut(s) 330, 493, 614
DpnI GATC 4 cut(s) 177, 533, 594, 729
DpnII GATC 4 cut(s) 175, 531, 592, 727
EaeI YGGCCR 2 cut(s) 763, 877
Eam1104I CTCTTC 3 cut(s) 378, 392, 791
EarI CTCTTC 3 cut(s) 378, 392, 791
Eco130I CCWWGG 1 cut(s) 991
Eco47I GGWCC 4 cut(s) 113, 215, 286, 1001
Eco88I CYCGRG 2 cut(s) 14, 1106
Eco91I GGTNACC 1 cut(s) 347
EcoNI CCTNNNNNAGG 1 cut(s) 1008
EcoO65I GGTNACC 1 cut(s) 347
EcoT14I CCWWGG 1 cut(s) 991
EcoT22I ATGCAT 1 cut(s) 723
ErhI CCWWGG 1 cut(s) 991
FaeI CATG 9 cut(s) 61, 95, 311, 538, 562, 770, 853, 884, 1153
FatI CATG 9 cut(s) 57, 91, 307, 534, 558, 766, 849, 880, 1149
FbaI TGATCA 1 cut(s) 592
Fnu4HI GCNGC 5 cut(s) 891, 1028, 1112, 1173, 1233
FokI GGATG 5 cut(s) 77, 351, 582, 767, 1079
Fsp4HI GCNGC 5 cut(s) 891, 1028, 1112, 1173, 1233
FspBI CTAG 4 cut(s) 290, 371, 992, 1176
GlaI GCGC 1 cut(s) 989
GluI GCNGC 5 cut(s) 891, 1028, 1112, 1173, 1233
HaeII RGCGCY 1 cut(s) 991
HaeIII GGCC 2 cut(s) 765, 879
HapII CCGG 5 cut(s) 196, 857, 1107, 1169, 1209
HhaI GCGC 1 cut(s) 990
Hin1II CATG 9 cut(s) 61, 95, 311, 538, 562, 770, 853, 884, 1153
Hin6I GCGC 1 cut(s) 988
HinP1I GCGC 1 cut(s) 988
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HindIII AAGCTT 2 cut(s) 611, 941
HinfI GANTC 5 cut(s) 34, 140, 278, 326, 1163
HpaII CCGG 5 cut(s) 196, 857, 1107, 1169, 1209
HphI GGTGA 4 cut(s) 359, 487, 689, 1025
Hpy166II GTNNAC 4 cut(s) 286, 667, 1001, 1162
Hpy188I TCNGA 7 cut(s) 11, 33, 103, 496, 927, 1094, 1182
Hpy188III TCNNGA 7 cut(s) 127, 332, 371, 394, 535, 1073, 1194
Hpy8I GTNNAC 4 cut(s) 286, 667, 1001, 1162
HpyAV CCTTC 7 cut(s) 228, 478, 505, 710, 967, 1177, 1213
HpyCH4III ACNGT 2 cut(s) 302, 841
HpyCH4IV ACGT 1 cut(s) 1101
HpyCH4V TGCA 8 cut(s) 21, 43, 57, 461, 721, 758, 1119, 1232
HpyF10VI GCNNNNNNNGC 1 cut(s) 152
HpyF3I CTNAG 3 cut(s) 330, 493, 614
HpySE526I ACGT 1 cut(s) 1101
Hsp92II CATG 9 cut(s) 61, 95, 311, 538, 562, 770, 853, 884, 1153
HspAI GCGC 1 cut(s) 988
Ksp22I TGATCA 1 cut(s) 592
Kzo9I GATC 4 cut(s) 175, 531, 592, 727
LguI GCTCTTC 1 cut(s) 791
LmnI GCTCC 1 cut(s) 671
Lsp1109I GCAGC 4 cut(s) 902, 1039, 1123, 1159
LweI GCATC 7 cut(s) 55, 88, 373, 423, 745, 918, 1057
MaeI CTAG 4 cut(s) 290, 371, 992, 1176
MaeII ACGT 1 cut(s) 1101
MaeIII GTNAC 2 cut(s) 198, 347
MalI GATC 4 cut(s) 177, 533, 594, 729
MboI GATC 4 cut(s) 175, 531, 592, 727
MflI RGATCY 2 cut(s) 175, 727
MlsI TGGCCA 2 cut(s) 765, 879
MluCI AATT 9 cut(s) 27, 51, 148, 563, 823, 1047, 1051, 1061, 1135
MluNI TGGCCA 2 cut(s) 765, 879
MlyI GAGTC 2 cut(s) 28, 1157
MmeI TCCRAC 1 cut(s) 1117
Mox20I TGGCCA 2 cut(s) 765, 879
Mph1103I ATGCAT 1 cut(s) 723
MroXI GAANNNNTTC 1 cut(s) 864
MscI TGGCCA 2 cut(s) 765, 879
MseI TTAA 5 cut(s) 581, 732, 776, 1050, 1240
MslI CAYNNNNRTG 1 cut(s) 708
Msp20I TGGCCA 2 cut(s) 765, 879
MspCI CTTAAG 1 cut(s) 731
MspI CCGG 5 cut(s) 196, 857, 1107, 1169, 1209
MspR9I CCNGG 3 cut(s) 1107, 1108, 1169
MvnI CGCG 1 cut(s) 506
MwoI GCNNNNNNNGC 1 cut(s) 152
NciI CCSGG 3 cut(s) 1107, 1108, 1169
NdeII GATC 4 cut(s) 175, 531, 592, 727
NlaIII CATG 9 cut(s) 61, 95, 311, 538, 562, 770, 853, 884, 1153
NlaIV GGNNCC 6 cut(s) 177, 336, 490, 673, 855, 959
NmeAIII GCCGAG 1 cut(s) 700
NmuCI GTSAC 2 cut(s) 198, 347
NsiI ATGCAT 1 cut(s) 723
NspI RCATGY 1 cut(s) 1153
PagI TCATGA 1 cut(s) 534
PciSI GCTCTTC 1 cut(s) 791
PdmI GAANNNNTTC 1 cut(s) 864
PfeI GAWTC 3 cut(s) 140, 278, 326
PflFI GACNNNGTC 1 cut(s) 353
PinAI ACCGGT 1 cut(s) 195
PkrI GCNGC 5 cut(s) 892, 1029, 1113, 1174, 1234
PleI GAGTC 2 cut(s) 28, 1157
PpsI GAGTC 2 cut(s) 28, 1157
PshBI ATTAAT 1 cut(s) 1050
PsiI TTATAA 1 cut(s) 267
Psp1406I AACGTT 1 cut(s) 1101
PspEI GGTNACC 1 cut(s) 347
PspN4I GGNNCC 6 cut(s) 177, 336, 490, 673, 855, 959
PspPI GGNCC 4 cut(s) 113, 215, 286, 1001
PstI CTGCAG 1 cut(s) 1234
PsuI RGATCY 2 cut(s) 175, 727
PsyI GACNNNGTC 1 cut(s) 353
RsaI GTAC 3 cut(s) 120, 172, 299
RsaNI GTAC 3 cut(s) 119, 171, 298
RseI CAYNNNNRTG 1 cut(s) 708
SapI GCTCTTC 1 cut(s) 791
SaqAI TTAA 5 cut(s) 581, 732, 776, 1050, 1240
SatI GCNGC 5 cut(s) 891, 1028, 1112, 1173, 1233
Sau3AI GATC 4 cut(s) 175, 531, 592, 727
Sau96I GGNCC 4 cut(s) 113, 215, 286, 1001
ScaI AGTACT 1 cut(s) 299
SchI GAGTC 2 cut(s) 28, 1157
ScrFI CCNGG 3 cut(s) 1107, 1108, 1169
SfaNI GCATC 7 cut(s) 55, 88, 373, 423, 745, 918, 1057
SfcI CTRYAG 3 cut(s) 69, 840, 1230
SinI GGWCC 4 cut(s) 113, 215, 286, 1001
SmaI CCCGGG 1 cut(s) 1108
SmiMI CAYNNNNRTG 1 cut(s) 708
SmlI CTYRAG 1 cut(s) 731
SmoI CTYRAG 1 cut(s) 731
Sse9I AATT 9 cut(s) 27, 51, 148, 563, 823, 1047, 1051, 1061, 1135
SsiI CCGC 3 cut(s) 77, 689, 961
SspMI CTAG 4 cut(s) 290, 371, 992, 1176
StyD4I CCNGG 3 cut(s) 1105, 1106, 1167
StyI CCWWGG 1 cut(s) 991
TaaI ACNGT 2 cut(s) 302, 841
TaiI ACGT 1 cut(s) 1104
TaqI TCGA 1 cut(s) 417
TasI AATT 9 cut(s) 27, 51, 148, 563, 823, 1047, 1051, 1061, 1135
TatI WGTACW 1 cut(s) 297
TfiI GAWTC 3 cut(s) 140, 278, 326
Tru1I TTAA 5 cut(s) 581, 732, 776, 1050, 1240
Tru9I TTAA 5 cut(s) 581, 732, 776, 1050, 1240
TscAI CASTG 4 cut(s) 141, 246, 288, 1121
TseFI GTSAC 2 cut(s) 198, 347
TseI GCWGC 5 cut(s) 890, 1027, 1111, 1172, 1232
Tsp45I GTSAC 2 cut(s) 198, 347
TspDTI ATGAA 9 cut(s) 175, 210, 324, 524, 551, 575, 592, 692, 1032
TspGWI ACGGA 2 cut(s) 188, 684
TspMI CCCGGG 1 cut(s) 1106
TspRI CASTG 4 cut(s) 141, 246, 288, 1121
Tth111I GACNNNGTC 1 cut(s) 353
Vha464I CTTAAG 1 cut(s) 731
VpaK11BI GGWCC 4 cut(s) 113, 215, 286, 1001
VspI ATTAAT 1 cut(s) 1050
XagI CCTNNNNNAGG 1 cut(s) 1008
XapI RAATTY 1 cut(s) 563
XbaI TCTAGA 1 cut(s) 370
XceI RCATGY 1 cut(s) 1153
XmaI CCCGGG 1 cut(s) 1106
XmaJI CCTAGG 1 cut(s) 991
XmnI GAANNNNTTC 1 cut(s) 864
XspI CTAG 4 cut(s) 290, 371, 992, 1176
ZrmI AGTACT 1 cut(s) 299
Zsp2I ATGCAT 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.