Rmu_sc0002986.1_g000039

Protein PHLOEM PROTEIN 2-LIKE A1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002986.1
OG0011580
Physical Location & Seq
Reverse (-)
177874 .. 180666
2793 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 522 bp
atgttcgaaattttcaatcatccggcatgcctgcagaaaatatggaaggagaagaaaactggctacaaatgcatgatgttgttcccaacgagtctcagtgttgttaatggtggtcctccatattgggtttgggattgttatgaggaaacaagtgatgagaacactgtggtagccaaactaaatgatgtatgttggctggaggtaacaggacacttcaacatgtcagagctctcagcaggaattgattatgagattgcgtacagagtcaaattaacgactgcagcaactggatgggaattccctgttactctcaatgtcagactcccgaatgattcaaaagtccgaaagcgccaatacagtctattcgagaagcccagagaagagtggatagacctggtcggtggccattttaaggtcaagaaaggagaaactggagaagcgcactttgatcttgctggaagtggcggacattggaagagtgggcttatcatccaaggtatcattatcaagccgaaaacttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

173

Amino Acids

19.72

Weight (kDa)

6.96

Isoelectric Point (pI)

23.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 23 - 171 1.5e-29 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 465
AcoI YGGCCR 1 cut(s) 403
AcsI RAATTY 2 cut(s) 9, 296
AfaI GTAC 1 cut(s) 260
AfiI CCNNNNNNNGG 1 cut(s) 412
AflIII ACRYGT 1 cut(s) 219
AgsI TTSAA 3 cut(s) 16, 217, 336
AjnI CCWGG 1 cut(s) 393
AluBI AGCT 1 cut(s) 229
AluI AGCT 1 cut(s) 229
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 98
AlwNI CAGNNNCTG 1 cut(s) 287
AoxI GGCC 1 cut(s) 403
ApeKI GCWGC 1 cut(s) 281
ApoI RAATTY 2 cut(s) 9, 296
AspLEI GCGC 2 cut(s) 351, 442
AspS9I GGNCC 1 cut(s) 113
AsuII TTCGAA 1 cut(s) 6
AvaII GGWCC 1 cut(s) 113
BalI TGGCCA 1 cut(s) 405
BanII GRGCYC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 1 cut(s) 293
BccI CCATC 1 cut(s) 285
BciT130I CCWGG 1 cut(s) 395
BcoDI GTCTC 1 cut(s) 98
BfmI CTRYAG 2 cut(s) 32, 279
BfoI RGCGCY 1 cut(s) 352
BisI GCNGC 1 cut(s) 282
BlsI GCNGC 1 cut(s) 283
Bme1390I CCNGG 1 cut(s) 395
Bme18I GGWCC 1 cut(s) 113
BmgT120I GGNCC 1 cut(s) 113
BmrFI CCNGG 1 cut(s) 395
BpmI CTGGAG 2 cut(s) 218, 453
Bpu14I TTCGAA 1 cut(s) 6
BsaJI CCNNGG 1 cut(s) 493
Bsc4I CCNNNNNNNGG 1 cut(s) 412
Bse1I ACTGG 3 cut(s) 64, 292, 436
BseBI CCWGG 1 cut(s) 395
BseDI CCNNGG 1 cut(s) 493
BseGI GGATG 3 cut(s) 19, 296, 489
BseLI CCNNNNNNNGG 1 cut(s) 412
BseMII CTCAG 2 cut(s) 109, 246
BseNI ACTGG 3 cut(s) 64, 292, 436
BseXI GCAGC 1 cut(s) 293
BshFI GGCC 1 cut(s) 405
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 1 cut(s) 23
BslI CCNNNNNNNGG 1 cut(s) 412
BsmAI GTCTC 1 cut(s) 98
BsnI GGCC 1 cut(s) 405
Bsp119I TTCGAA 1 cut(s) 6
Bsp1286I GDGCHC 1 cut(s) 231
Bsp143I GATC 1 cut(s) 448
BspACI CCGC 1 cut(s) 465
BspANI GGCC 1 cut(s) 405
BspCNI CTCAG 2 cut(s) 108, 245
BspMAI CTGCAG 2 cut(s) 36, 283
BspT104I TTCGAA 1 cut(s) 6
BsrI ACTGG 3 cut(s) 64, 292, 436
BssECI CCNNGG 1 cut(s) 493
BssMI GATC 1 cut(s) 448
BssT1I CCWWGG 1 cut(s) 493
Bst2UI CCWGG 1 cut(s) 395
Bst4CI ACNGT 2 cut(s) 166, 359
Bst6I CTCTTC 2 cut(s) 375, 470
BstBI TTCGAA 1 cut(s) 6
BstC8I GCNNGC 2 cut(s) 28, 32
BstDEI CTNAG 2 cut(s) 95, 232
BstF5I GGATG 3 cut(s) 19, 296, 489
BstH2I RGCGCY 1 cut(s) 352
BstHHI GCGC 2 cut(s) 351, 442
BstKTI GATC 1 cut(s) 451
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 1 cut(s) 448
BstMWI GCNNNNNNNGC 1 cut(s) 69
BstNI CCWGG 1 cut(s) 395
BstNSI RCATGY 2 cut(s) 30, 223
BstSCI CCNGG 1 cut(s) 393
BstSFI CTRYAG 2 cut(s) 32, 279
BstV1I GCAGC 1 cut(s) 293
BsuRI GGCC 1 cut(s) 405
BtsCI GGATG 3 cut(s) 19, 296, 489
BtsIMutI CAGTG 2 cut(s) 103, 162
Cac8I GCNNGC 2 cut(s) 28, 32
CaiI CAGNNNCTG 1 cut(s) 287
CfoI GCGC 2 cut(s) 351, 442
Cfr13I GGNCC 1 cut(s) 113
CsiI ACCWGGT 1 cut(s) 393
Csp6I GTAC 1 cut(s) 259
CviAII CATG 3 cut(s) 27, 73, 220
CviJI RGCY 8 cut(s) 63, 173, 196, 229, 373, 405, 484, 511
CviKI_1 RGCY 8 cut(s) 63, 173, 196, 229, 373, 405, 484, 511
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 2 cut(s) 95, 232
DpnI GATC 1 cut(s) 450
DpnII GATC 1 cut(s) 448
EaeI YGGCCR 1 cut(s) 403
Eam1104I CTCTTC 2 cut(s) 375, 470
EarI CTCTTC 2 cut(s) 375, 470
EciI GGCGGA 1 cut(s) 480
Ecl136II GAGCTC 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 493
Eco24I GRGCYC 1 cut(s) 231
Eco47I GGWCC 1 cut(s) 113
Eco53kI GAGCTC 1 cut(s) 229
EcoICRI GAGCTC 1 cut(s) 229
EcoRI GAATTC 1 cut(s) 296
EcoRII CCWGG 1 cut(s) 393
EcoT14I CCWWGG 1 cut(s) 493
EcoT22I ATGCAT 1 cut(s) 74
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 493
FaeI CATG 3 cut(s) 30, 76, 223
FaiI YATR 8 cut(s) 28, 43, 74, 121, 141, 190, 221, 249
FatI CATG 3 cut(s) 26, 72, 219
Fnu4HI GCNGC 1 cut(s) 282
FokI GGATG 3 cut(s) 6, 303, 476
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 1 cut(s) 282
GlaI GCGC 2 cut(s) 350, 441
GluI GCNGC 1 cut(s) 282
GsuI CTGGAG 2 cut(s) 218, 453
HaeII RGCGCY 1 cut(s) 352
HaeIII GGCC 1 cut(s) 405
HapII CCGG 1 cut(s) 23
HhaI GCGC 2 cut(s) 351, 442
Hin1II CATG 3 cut(s) 30, 76, 223
Hin6I GCGC 2 cut(s) 349, 440
HinP1I GCGC 2 cut(s) 349, 440
HinfI GANTC 4 cut(s) 91, 264, 321, 332
HpaII CCGG 1 cut(s) 23
Hpy188I TCNGA 3 cut(s) 226, 320, 344
Hpy188III TCNNGA 3 cut(s) 325, 367, 418
HpyAV CCTTC 1 cut(s) 40
HpyCH4III ACNGT 2 cut(s) 166, 359
HpyCH4V TGCA 3 cut(s) 34, 72, 281
HpyF10VI GCNNNNNNNGC 1 cut(s) 69
HpyF3I CTNAG 2 cut(s) 95, 232
Hsp92II CATG 3 cut(s) 30, 76, 223
HspAI GCGC 2 cut(s) 349, 440
Kzo9I GATC 1 cut(s) 448
Lsp1109I GCAGC 1 cut(s) 293
MabI ACCWGGT 1 cut(s) 393
MaeIII GTNAC 2 cut(s) 202, 304
MalI GATC 1 cut(s) 450
MboI GATC 1 cut(s) 448
MboII GAAGA 3 cut(s) 64, 392, 487
MhlI GDGCHC 1 cut(s) 231
MlsI TGGCCA 1 cut(s) 405
MluCI AATT 4 cut(s) 9, 240, 269, 296
MluNI TGGCCA 1 cut(s) 405
MlyI GAGTC 3 cut(s) 100, 273, 315
MnlI CCTC 3 cut(s) 126, 136, 193
Mox20I TGGCCA 1 cut(s) 405
Mph1103I ATGCAT 1 cut(s) 74
MscI TGGCCA 1 cut(s) 405
MseI TTAA 4 cut(s) 105, 272, 411, 520
Msp20I TGGCCA 1 cut(s) 405
MspI CCGG 1 cut(s) 23
MspR9I CCNGG 1 cut(s) 395
MvaI CCWGG 1 cut(s) 395
MwoI GCNNNNNNNGC 1 cut(s) 69
NdeII GATC 1 cut(s) 448
NlaIII CATG 3 cut(s) 30, 76, 223
NsiI ATGCAT 1 cut(s) 74
NspI RCATGY 2 cut(s) 30, 223
NspV TTCGAA 1 cut(s) 6
PaeI GCATGC 1 cut(s) 30
PciI ACATGT 1 cut(s) 219
PfeI GAWTC 1 cut(s) 332
PflFI GACNNNGTC 1 cut(s) 395
PkrI GCNGC 1 cut(s) 283
PleI GAGTC 3 cut(s) 99, 272, 315
PpsI GAGTC 3 cut(s) 99, 272, 315
PscI ACATGT 1 cut(s) 219
Psp124BI GAGCTC 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 393
PspGI CCWGG 1 cut(s) 393
PspPI GGNCC 1 cut(s) 113
PstI CTGCAG 2 cut(s) 36, 283
PstNI CAGNNNCTG 1 cut(s) 287
PsyI GACNNNGTC 1 cut(s) 395
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
SacI GAGCTC 1 cut(s) 231
SaqAI TTAA 4 cut(s) 105, 272, 411, 520
SatI GCNGC 1 cut(s) 282
Sau3AI GATC 1 cut(s) 448
Sau96I GGNCC 1 cut(s) 113
SchI GAGTC 3 cut(s) 100, 273, 315
ScrFI CCNGG 1 cut(s) 395
SduI GDGCHC 1 cut(s) 231
SetI ASST 5 cut(s) 204, 231, 396, 417, 499
SexAI ACCWGGT 1 cut(s) 393
SfcI CTRYAG 2 cut(s) 32, 279
SfuI TTCGAA 1 cut(s) 6
SinI GGWCC 1 cut(s) 113
SphI GCATGC 1 cut(s) 30
Sse9I AATT 4 cut(s) 9, 240, 269, 296
SsiI CCGC 1 cut(s) 465
SstI GAGCTC 1 cut(s) 231
StyD4I CCNGG 1 cut(s) 393
StyI CCWWGG 1 cut(s) 493
TaaI ACNGT 2 cut(s) 166, 359
TaqI TCGA 2 cut(s) 6, 366
TasI AATT 4 cut(s) 9, 240, 269, 296
TfiI GAWTC 1 cut(s) 332
Tru1I TTAA 4 cut(s) 105, 272, 411, 520
Tru9I TTAA 4 cut(s) 105, 272, 411, 520
TscAI CASTG 2 cut(s) 103, 169
TseI GCWGC 1 cut(s) 281
TspRI CASTG 2 cut(s) 103, 169
Tth111I GACNNNGTC 1 cut(s) 395
VpaK11BI GGWCC 1 cut(s) 113
XapI RAATTY 2 cut(s) 9, 296
XceI RCATGY 2 cut(s) 30, 223
XcmI CCANNNNNNNNNTGG 1 cut(s) 126
Zsp2I ATGCAT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.