Rmu_sc0003296.1_g000006

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003296.1
OG0005240
Physical Location & Seq
Forward (+)
20853 .. 35067
14215 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5520 bp
atgatgacgtggaacggcgcccgcgacatagtgctcgactcggatgacgttccgttcgggaccggtgagtggtacctctacgccggaatctcttgcgtgctggttttgttcgccggaatcatgtccggccttacgctcggattgatgtcgctcaatctcgtcgacctcgagattctccagcggagcggttccaccgatcagaaaaagcaagccgctgcgattctacctgtggtgaaaaagcagcaccagcttttggtcactttgcttctctgcaatgcttgtgccatggaggcccttcctttataccttgatgaaatctttcaccctgttgttgccgttttgctgtctgtaacatttgttctaattttcggagagataattccacaagctatatgctcaagatatggactgtctgttggttcaaacttcgtttggcttgttcgcattctgatgatcatctgctatccaatcgcgtacccaattggaaaggttctggatcttgttctcggacacggggatgatttgttcaggcgagcacagttgaaagctctcgtctctattcacggccttgaggctggaaaaggaggtgaactaacacatgatgagacaacgatcatcagtggagcgctagatttaactgaaaagactgctgaggaggctatgacacctattgaatcaacattttccttggatgttaattcaaagttagactgggaagcaattgggaaaattcttgcacgtggtcatagtagagtccctgtctattctggaaatcccaaaaacataatcggcctcttactggtgaaaagtctcctcaccgtacgagcagaaacagagactccagttagtgcagtttccattcgtagaatgcctagggttccagccgatatgcctttgtatgatatacttaatgagtttcaaaaaggaagcagtcacctggctgctgtagtgaagatcaaaggaaaaaccaagaatcctctactttttgctgatagagagaaactcgaggaggccagactaaccaatggatgtgttgccattgatgtcgataaacgtccaaggtctctgacaaacatgccaactgttcaagaaaatgatgctactacaaatggcatttcaaattcatcagatgatattgaagatggggaggtgattggaatcataaccctggaggatgtttttgaagaacttctgcaagaagaaattgtagatgagacagatgtatatattgatgtacataagaggatacgtgtggctgctgctgcagctgcttcatccatgtccagagctcagtcaagtcagaagttgattagtcaaaagcatgcaggagctacaaataagcacggacaaaccccaaagaagtctggggaggatgatgcacattcgacgaggttttcagggagttcacgggagcctcttctaggtattagtgtgttggatatggggtttaatgcggtttttagctccttgaaggaggtttttcctcaggttgattttcgtgttctgaaagctgttgctatagaaaattctaatgatcttgacgcagctgtcaatgatgtgctgaatgaggttctccctttcttgaccaaacatcctgagagtcctgcaaaggttcagactcctagcagccaaccaactgcagttgaacctgaggagcagagtaaagaattaaatcacccgcaggtagttaaggaagtagaggttgagtcttttccagcagcaagatccactgatgttgacaatgccaacactactgatcaaacagaatttacaagcggtgcctcccatgaggtttgtacactggtagatgacactaattcatcacagaatctccttgcctcttcagatggagatcaagcaactggccatgatgaaaaagaatttgcgaacactggaagtgaagaactggagcgacgtggaaagaagattaatgaggatgatgacactttcatattgccagaggggattagtgtgttggatatggggtttaatgcggtttttagctccttgaaggaggtttttcctcaggttgattttcgtgttctgaaagctgttgctatagaaaattctaatgatcttgacgcagctgtcaatgatgtgctgaatgaggttctccccttcttgaccaaacatcctgagagtcctgcaaaggttcagactcctagcagccaaccaactgcagttgaacctgaggagcagagtaaagaattaaatcacccgcaggtagttgaggaagtagaggttgagtcttttccagcagcaagatccactgatgttgacaatgccaacactactgatcaaacagaatttacaagtggtgcctcccatgaggtttgtacactggtagatgacactaattcatcacagaatctccttgcctcttcagatggagatcaagcaactggccatgatgaaaaagaatttgcgaacactggaagtgaagaactggagcgacgtggaaagaagattaatgaggatgatgacactttcatattgccagagggggtagaaacatttctgagtgatactcggctttataccgaaacaacagcagctggtaatgttgaagctgggcttcagcagtcttcatctggaatggcaaccagttcagtacatgaaaaagagggggttcatgacggcttggtcaatgtgccctatgaatggaaggattttgatttttctcagactgatgatctgtctctgttggagggtgaaagttctattgttcagctggatcctccttttccagaacatgcaacagaaactgtcctttccaaggaagacgagtctttctgtaatatggctgatatgaaggagtccaccacgaatagcatatgcaatattgatgttctcgaagagagcattgaagatgctaagcataacaagattagtgtgttggatatggggtttaatgcggtttttagctccttgaaggaggtttttcctcaggttgattttcgtgttctgaaagctgttgctatagaaaattctaatgatcttgacgcagctgtcaatgatgtgctgaatgaggttctccctttcttgaccaaacatcctgagagtcctgcaaaggttcagactcctagcagccaaccaactgcagttgaacctgaggagcagagtaaagaattaaatcacccgcaggtagttgaggaagtagaggttgagtcttttccagcagcaagatccactgatgttgacaatgccaacactactgatcaaacagaatttacaagtggtgcctcccatgaggtttgtacactggtagatgacactaattcatcacagaatctccttgcctcttcagatggagatcaagcaactggccatgatgaaaaagaatttgcgaacactggaagtgaagaactggagcgacgtggaaagaagattaatgaggatgatgacactttcatattgccagagggggtagaaacatttctgagtgatactcggctttataccgaaacaacagcagctggtaatgtcgaagctgggcttcagcagtcttcatctggaatggcaaccagttcagtacatgaaaaagagggggttcatgacggcttggtcaatgtgccctatgaatggaaggattttgatttttctcagactgatgatctgtctctgttggagggtgaaagttctattgttcagctggatcctccttttccagaacatgcaacagaaactgtcctttccaaggaagacgagtctttctgtaatatggctgatatgaaggagtccaccacgaatagcatatgcaatattgatgttctcgaagagagcattgaagatgctaagcataacaaggttgattttcgtgttctgaaagctgttgctatagaaaattctaatgatcttgacgcagctgtcaatgatgtgctgaatgaggttctccccttcttgaccaaacatcctgatagtcctgcaaaggttcagactcctagcagccaaccaactgcagttgaacctgaggagcagagtaaagaattaaatcacccgcaggtagttgaggaagtagaggttgagtcttttccagcagcaagatccactgatgttgacaatgccaacactactgatcaaacagaatttacaagtggtgcctcccatgaggtttgtacactggtagatgacacaaattcatcacagaatctccttgcctcttcagatggagatcaagcaactggccatgatgaaaaagaatttttgaacactggaagtgaagaactggagcgacgtggaaagaagattaatgaggatgatgacactttcatattgccagagggggtagaaacatttctgagtgatactcggctttataccgaaacaacagcagctggtaatgtcgaagctgggcttcagcagtcttcatctggaatggcaaccagttcagtacatgaaaaagagggggttcatgacggcttggtcaatgtgccctatgaatggaaggattttgatttttctcagactgatgatctgtctctgttggagggtgaaagttctattgttcagctggatcctccttttccagaacatgcaacagaaactgtcctttccaaggaagacgagtctttctgtaatatggctgatatgaaggagtccaccacgaatagcatatgcaatattgatgttctcgaagagagcattgaagatgctaagcataacaagagaacattgttttcagccatggagtctgttatcaccatgatgagagaagtggaactggaagagaaagctgtggatcgtgtcacagaggaagccgcaaggggaggtcaggatattatggttaaggtggaggaactaaaactgatgttggcacatgcgaaggatgcaaatgacatgcatgctggtgaagtttatggggagaaggcaattttagcaactgaagttagagagcttcaatgtcgattgctcagtttgtcagatgaaagggataagtcacttgcaatccttgacgagatgcgtgaaacacttgaagcacgactaactggagttgcagagttgagaaaaatggccgaggatgaaaagctagaaaaggaagaatctgcacggaaagctcttgctgaacaagaagccatcatggagaaagtggttgaggagtccaagacactgcaacaggcagcggaggaaaattccaagttgcgggagtttctgatggaccgtggccacattgtagatatgctacaaggagaaatctctgtaatttgtcaggatgtcaagttgctgaaagagaagtttgatgatcgtgttcctctaagccagtctgtttcatctagccagacaagttgcatcttagcttcttcaggctcatccttgaaaagcgcggcatctgacttgcttctagaacgagttgggtcagttgggtcatccgagtcaccaaagaccgtagaaagggcaagtcctgttgcttcagttgacggtctctcaccaaaatacagactagaagataaaacagtcaaggtcgataaacaagctcttatggccgatgggtgggatgtctttgacgaagatctcttggagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
DUF21

Protein Analysis

1839

Amino Acids

201.13

Weight (kDa)

4.4

Isoelectric Point (pI)

46.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 30 - 191 3.2e-33 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 7 cut(s) 1547, 2087, 2642, 3008, 3563, 3866, 4421
Acc36I ACCTGC 4 cut(s) 1672, 2212, 3133, 3991
Acc65I GGTACC 1 cut(s) 72
AccB1I GGYRCC 6 cut(s) 17, 72, 1778, 2318, 3239, 4097
AccB7I CCANNNNNTGG 1 cut(s) 253
AccBSI CCGCTC 1 cut(s) 186
AccI GTMKAC 1 cut(s) 162
AccII CGCG 3 cut(s) 24, 473, 5321
AcoI YGGCCR 7 cut(s) 1864, 2404, 3325, 4183, 5010, 5161, 5478
AcvI CACGTG 1 cut(s) 740
AcyI GRCGYC 1 cut(s) 18
AfeI AGCGCT 1 cut(s) 627
AflIII ACRYGT 1 cut(s) 1249
AgeI ACCGGT 1 cut(s) 62
AjiI CACGTC 5 cut(s) 9, 1916, 2456, 3377, 4235
AjnI CCWGG 2 cut(s) 936, 1167
AloI GAACNNNNNNTCC 2 cut(s) 509, 541
Alw21I GWGCWC 3 cut(s) 36, 538, 1291
AlwNI CAGNNNCTG 6 cut(s) 2555, 2765, 3476, 3686, 4334, 4544
Ama87I CYCGRG 2 cut(s) 167, 1004
Aor51HI AGCGCT 1 cut(s) 627
ArsI GACNNNNNNTTYG 2 cut(s) 38, 70
AseI ATTAAT 4 cut(s) 1929, 2469, 3390, 4248
AsiGI ACCGGT 1 cut(s) 62
Asp700I GAANNNNTTC 5 cut(s) 318, 1188, 2514, 3435, 4293
Asp718I GGTACC 1 cut(s) 72
AspA2I CCTAGG 1 cut(s) 872
AspLEI GCGC 3 cut(s) 20, 628, 5321
AspS9I GGNCC 3 cut(s) 60, 292, 5155
AvaI CYCGRG 2 cut(s) 167, 1004
AvaII GGWCC 2 cut(s) 60, 5155
AvrII CCTAGG 1 cut(s) 872
AxyI CCTNAGG 7 cut(s) 1485, 1650, 2025, 2190, 2946, 3111, 3969
BaeGI GKGCMC 3 cut(s) 2655, 3576, 4434
BalI TGGCCA 5 cut(s) 1866, 2406, 3327, 4185, 5163
BamHI GGATCC 3 cut(s) 2734, 3655, 4513
BanI GGYRCC 6 cut(s) 17, 72, 1778, 2318, 3239, 4097
BanII GRGCYC 1 cut(s) 1291
BbrPI CACGTG 1 cut(s) 740
BbsI GAAGAC 6 cut(s) 2577, 2787, 3498, 3708, 4356, 4566
Bbv12I GWGCWC 3 cut(s) 36, 538, 1291
BbvCI CCTCAGC 1 cut(s) 651
BccI CCATC 8 cut(s) 1136, 1841, 2381, 3302, 4160, 5081, 5146, 5477
BceAI ACGGC 6 cut(s) 31, 320, 580, 2653, 3574, 4432
BciT130I CCWGG 2 cut(s) 938, 1169
BciVI GTATCC 1 cut(s) 1239
BclI TGATCA 5 cut(s) 453, 1756, 2296, 3217, 4075
BfoI RGCGCY 2 cut(s) 21, 629
BfuAI ACCTGC 4 cut(s) 1672, 2212, 3133, 3991
BfuI GTATCC 1 cut(s) 1239
BglI GCCNNNNNGGC 1 cut(s) 290
BglII AGATCT 1 cut(s) 5506
BlnI CCTAGG 1 cut(s) 872
BlpI GCTNAGC 3 cut(s) 2874, 3795, 4653
Bme1390I CCNGG 2 cut(s) 938, 1169
Bme18I GGWCC 2 cut(s) 60, 5155
BmeT110I CYCGRG 2 cut(s) 167, 1004
BmgBI CACGTC 5 cut(s) 9, 1916, 2456, 3377, 4235
BmgT120I GGNCC 3 cut(s) 60, 292, 5155
BmrFI CCNGG 2 cut(s) 938, 1169
BmrI ACTGGG 1 cut(s) 721
BmsI GCATC 9 cut(s) 1087, 1366, 2860, 3781, 4639, 4816, 4947, 5295, 5333
BmuI ACTGGG 1 cut(s) 721
BpiI GAAGAC 6 cut(s) 2577, 2787, 3498, 3708, 4356, 4566
BplI GAGNNNNNCTC 8 cut(s) 987, 1019, 2512, 2544, 3433, 3465, 4291, 4323
BpmI CTGGAG 8 cut(s) 161, 825, 1190, 1928, 2468, 3389, 4247, 5007
Bpu10I CCTNAGC 1 cut(s) 651
Bpu1102I GCTNAGC 3 cut(s) 2874, 3795, 4653
BpuEI CTTGAG 2 cut(s) 382, 590
BsaAI YACGTR 2 cut(s) 740, 1250
BsaBI GATNNNNATC 6 cut(s) 455, 1157, 1851, 2391, 3312, 4170
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 2 cut(s) 1068, 5423
BsaWI WCCGGW 1 cut(s) 62
Bse118I RCCGGY 1 cut(s) 62
Bse21I CCTNAGG 7 cut(s) 1485, 1650, 2025, 2190, 2946, 3111, 3969
Bse3DI GCAATG 1 cut(s) 280
Bse8I GATNNNNATC 6 cut(s) 455, 1157, 1851, 2391, 3312, 4170
BseBI CCWGG 2 cut(s) 938, 1169
BseJI GATNNNNATC 6 cut(s) 455, 1157, 1851, 2391, 3312, 4170
BseMI GCAATG 1 cut(s) 280
BseRI GAGGAG 8 cut(s) 668, 803, 1022, 1667, 2207, 3128, 3986, 5108
BseSI GKGCMC 3 cut(s) 2655, 3576, 4434
BseYI CCCAGC 3 cut(s) 2570, 3491, 4349
BsgI GTGCAG 2 cut(s) 870, 5028
Bsh1236I CGCG 3 cut(s) 24, 473, 5321
BshNI GGYRCC 6 cut(s) 17, 72, 1778, 2318, 3239, 4097
BshTI ACCGGT 1 cut(s) 62
BsiHKAI GWGCWC 3 cut(s) 36, 538, 1291
BsiHKCI CYCGRG 2 cut(s) 167, 1004
BsiSI CCGG 4 cut(s) 63, 84, 114, 126
BsiWI CGTACG 1 cut(s) 820
BslFI GGGAC 2 cut(s) 73, 740
BsmBI CGTCTC 1 cut(s) 559
BsmFI GGGAC 2 cut(s) 73, 740
BsmI GAATGC 2 cut(s) 444, 873
Bso31I GGTCTC 2 cut(s) 1068, 5423
BsoBI CYCGRG 2 cut(s) 167, 1004
Bsp1286I GDGCHC 6 cut(s) 36, 538, 1291, 2655, 3576, 4434
Bsp1407I TGTACA 5 cut(s) 1234, 1796, 2336, 3257, 4115
Bsp1720I GCTNAGC 3 cut(s) 2874, 3795, 4653
Bsp19I CCATGG 2 cut(s) 285, 4683
BspFNI CGCG 3 cut(s) 24, 473, 5321
BspHI TCATGA 3 cut(s) 2632, 3553, 4411
BspMAI CTGCAG 5 cut(s) 1267, 1642, 2182, 3103, 3961
BspMI ACCTGC 4 cut(s) 1672, 2212, 3133, 3991
BspT107I GGYRCC 6 cut(s) 17, 72, 1778, 2318, 3239, 4097
BspTNI GGTCTC 2 cut(s) 1068, 5423
BsrBI CCGCTC 1 cut(s) 186
BsrDI GCAATG 1 cut(s) 280
BsrFI RCCGGY 1 cut(s) 62
BsrGI TGTACA 5 cut(s) 1234, 1796, 2336, 3257, 4115
BssAI RCCGGY 1 cut(s) 62
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 8 cut(s) 285, 687, 872, 1058, 2775, 3696, 4554, 4683
Bst2UI CCWGG 2 cut(s) 938, 1169
Bst6I CTCTTC 9 cut(s) 1422, 1846, 2386, 2850, 3307, 3771, 4165, 4629, 4719
BstACI GRCGYC 1 cut(s) 18
BstAUI TGTACA 5 cut(s) 1234, 1796, 2336, 3257, 4115
BstBAI YACGTR 2 cut(s) 740, 1250
BstC8I GCNNGC 6 cut(s) 22, 98, 210, 534, 1323, 4842
BstDSI CCRYGG 3 cut(s) 285, 4683, 5158
BstENI CCTNNNNNAGG 4 cut(s) 1419, 2774, 3695, 4553
BstFNI CGCG 3 cut(s) 24, 473, 5321
BstH2I RGCGCY 2 cut(s) 21, 629
BstHHI GCGC 3 cut(s) 20, 628, 5321
BstNI CCWGG 2 cut(s) 938, 1169
BstNSI RCATGY 8 cut(s) 1078, 1325, 2756, 3677, 4535, 4820, 4840, 4844
BstSCI CCNGG 2 cut(s) 936, 1167
BstSLI GKGCMC 3 cut(s) 2655, 3576, 4434
BstUI CGCG 3 cut(s) 24, 473, 5321
BstV2I GAAGAC 6 cut(s) 2577, 2787, 3498, 3708, 4356, 4566
BstX2I RGATCY 9 cut(s) 496, 1724, 2264, 2734, 3185, 3655, 4043, 4513, 5506
BstYI RGATCY 9 cut(s) 496, 1724, 2264, 2734, 3185, 3655, 4043, 4513, 5506
Bsu36I CCTNAGG 7 cut(s) 1485, 1650, 2025, 2190, 2946, 3111, 3969
BsuI GTATCC 1 cut(s) 1239
BtgI CCRYGG 3 cut(s) 285, 4683, 5158
BtrI CACGTC 5 cut(s) 9, 1916, 2456, 3377, 4235
BtsI GCAGTG 1 cut(s) 5105
BveI ACCTGC 4 cut(s) 1672, 2212, 3133, 3991
Cac8I GCNNGC 6 cut(s) 22, 98, 210, 534, 1323, 4842
CaiI CAGNNNCTG 6 cut(s) 2555, 2765, 3476, 3686, 4334, 4544
CciI TCATGA 3 cut(s) 2632, 3553, 4411
CfoI GCGC 3 cut(s) 20, 628, 5321
Cfr10I RCCGGY 1 cut(s) 62
Cfr13I GGNCC 3 cut(s) 60, 292, 5155
CseI GACGC 4 cut(s) 1550, 2090, 3011, 3869
CspAI ACCGGT 1 cut(s) 62
CspCI CAANNNNNGTGG 8 cut(s) 1717, 1752, 2257, 2292, 3178, 3213, 4036, 4071
DinI GGCGCC 1 cut(s) 19
DrdI GACNNNNNNGTC 7 cut(s) 1547, 2087, 2642, 3008, 3563, 3866, 4421
DseDI GACNNNNNNGTC 7 cut(s) 1547, 2087, 2642, 3008, 3563, 3866, 4421
EaeI YGGCCR 7 cut(s) 1864, 2404, 3325, 4183, 5010, 5161, 5478
Eam1104I CTCTTC 9 cut(s) 1422, 1846, 2386, 2850, 3307, 3771, 4165, 4629, 4719
EarI CTCTTC 9 cut(s) 1422, 1846, 2386, 2850, 3307, 3771, 4165, 4629, 4719
Ecl136II GAGCTC 1 cut(s) 1289
Eco130I CCWWGG 8 cut(s) 285, 687, 872, 1058, 2775, 3696, 4554, 4683
Eco24I GRGCYC 1 cut(s) 1291
Eco31I GGTCTC 2 cut(s) 1068, 5423
Eco47I GGWCC 2 cut(s) 60, 5155
Eco47III AGCGCT 1 cut(s) 627
Eco53kI GAGCTC 1 cut(s) 1289
Eco72I CACGTG 1 cut(s) 740
Eco81I CCTNAGG 7 cut(s) 1485, 1650, 2025, 2190, 2946, 3111, 3969
Eco88I CYCGRG 2 cut(s) 167, 1004
EcoICRI GAGCTC 1 cut(s) 1289
EcoNI CCTNNNNNAGG 4 cut(s) 1419, 2774, 3695, 4553
EcoO109I RGGNCCY 1 cut(s) 292
EcoRII CCWGG 2 cut(s) 936, 1167
EcoT14I CCWWGG 8 cut(s) 285, 687, 872, 1058, 2775, 3696, 4554, 4683
EcoT22I ATGCAT 1 cut(s) 4842
EcoT38I GRGCYC 1 cut(s) 1291
EgeI GGCGCC 1 cut(s) 19
EheI GGCGCC 1 cut(s) 19
ErhI CCWWGG 8 cut(s) 285, 687, 872, 1058, 2775, 3696, 4554, 4683
Esp3I CGTCTC 1 cut(s) 559
FalI AAGNNNNNCTT 6 cut(s) 2559, 2591, 3480, 3512, 4338, 4370
FaqI GGGAC 2 cut(s) 73, 740
FauI CCCGC 6 cut(s) 29, 1686, 2226, 3147, 4005, 5133
FauNDI CATATG 3 cut(s) 2834, 3755, 4613
FbaI TGATCA 5 cut(s) 453, 1756, 2296, 3217, 4075
FblI GTMKAC 1 cut(s) 162
FriOI GRGCYC 1 cut(s) 1291
GlaI GCGC 3 cut(s) 19, 627, 5320
GsaI CCCAGC 3 cut(s) 2574, 3495, 4353
GsuI CTGGAG 8 cut(s) 161, 825, 1190, 1928, 2468, 3389, 4247, 5007
HaeII RGCGCY 2 cut(s) 21, 629
HapII CCGG 4 cut(s) 63, 84, 114, 126
HgaI GACGC 4 cut(s) 1550, 2090, 3011, 3869
HhaI GCGC 3 cut(s) 20, 628, 5321
Hin1I GRCGYC 1 cut(s) 18
Hin6I GCGC 3 cut(s) 18, 626, 5319
HinP1I GCGC 3 cut(s) 18, 626, 5319
HincII GTYRAC 6 cut(s) 163, 1738, 2278, 3199, 4057, 5413
HindII GTYRAC 6 cut(s) 163, 1738, 2278, 3199, 4057, 5413
HpaII CCGG 4 cut(s) 63, 84, 114, 126
Hpy99I CGWCG 6 cut(s) 164, 1390, 1917, 2457, 3378, 4236
HpyCH4IV ACGT 9 cut(s) 8, 48, 739, 1054, 1249, 1915, 2455, 3376, 4234
HpySE526I ACGT 9 cut(s) 8, 48, 739, 1054, 1249, 1915, 2455, 3376, 4234
Hsp92I GRCGYC 1 cut(s) 18
HspAI GCGC 3 cut(s) 18, 626, 5319
KasI GGCGCC 1 cut(s) 17
KpnI GGTACC 1 cut(s) 76
Ksp22I TGATCA 5 cut(s) 453, 1756, 2296, 3217, 4075
LweI GCATC 9 cut(s) 1087, 1366, 2860, 3781, 4639, 4816, 4947, 5295, 5333
MaeII ACGT 9 cut(s) 8, 48, 739, 1054, 1249, 1915, 2455, 3376, 4234
MaeIII GTNAC 6 cut(s) 256, 349, 932, 4744, 4935, 5370
MbiI CCGCTC 1 cut(s) 186
MfeI CAATTG 2 cut(s) 480, 720
MflI RGATCY 9 cut(s) 496, 1724, 2264, 2734, 3185, 3655, 4043, 4513, 5506
MhlI GDGCHC 6 cut(s) 36, 538, 1291, 2655, 3576, 4434
MlsI TGGCCA 5 cut(s) 1866, 2406, 3327, 4185, 5163
MluNI TGGCCA 5 cut(s) 1866, 2406, 3327, 4185, 5163
Mly113I GGCGCC 1 cut(s) 18
MmeI TCCRAC 6 cut(s) 1416, 1956, 2685, 2877, 3606, 4464
Mox20I TGGCCA 5 cut(s) 1866, 2406, 3327, 4185, 5163
Mph1103I ATGCAT 1 cut(s) 4842
MroXI GAANNNNTTC 5 cut(s) 318, 1188, 2514, 3435, 4293
MscI TGGCCA 5 cut(s) 1866, 2406, 3327, 4185, 5163
MslI CAYNNNNRTG 4 cut(s) 449, 516, 4703, 4845
Msp20I TGGCCA 5 cut(s) 1866, 2406, 3327, 4185, 5163
MspI CCGG 4 cut(s) 63, 84, 114, 126
MspR9I CCNGG 2 cut(s) 938, 1169
MunI CAATTG 2 cut(s) 480, 720
Mva1269I GAATGC 2 cut(s) 444, 873
MvaI CCWGG 2 cut(s) 938, 1169
MvnI CGCG 3 cut(s) 24, 473, 5321
NarI GGCGCC 1 cut(s) 18
NcoI CCATGG 2 cut(s) 285, 4683
NdeI CATATG 3 cut(s) 2834, 3755, 4613
NmeAIII GCCGAG 4 cut(s) 2509, 3430, 4288, 5038
NmuCI GTSAC 5 cut(s) 256, 932, 4744, 4935, 5370
NsiI ATGCAT 1 cut(s) 4842
NspI RCATGY 8 cut(s) 1078, 1325, 2756, 3677, 4535, 4820, 4840, 4844
PaeI GCATGC 2 cut(s) 1325, 4844
PaeR7I CTCGAG 2 cut(s) 167, 1004
PagI TCATGA 3 cut(s) 2632, 3553, 4411
PcsI WCGNNNNNNNCGW 2 cut(s) 21, 165
PctI GAATGC 2 cut(s) 444, 873
PdmI GAANNNNTTC 5 cut(s) 318, 1188, 2514, 3435, 4293
Pfl23II CGTACG 1 cut(s) 820
PflMI CCANNNNNTGG 1 cut(s) 253
PinAI ACCGGT 1 cut(s) 62
PluTI GGCGCC 1 cut(s) 21
PmaCI CACGTG 1 cut(s) 740
PmlI CACGTG 1 cut(s) 740
Ppu21I YACGTR 2 cut(s) 740, 1250
PshBI ATTAAT 4 cut(s) 1929, 2469, 3390, 4248
Psp124BI GAGCTC 1 cut(s) 1291
Psp6I CCWGG 2 cut(s) 936, 1167
PspCI CACGTG 1 cut(s) 740
PspFI CCCAGC 3 cut(s) 2570, 3491, 4349
PspGI CCWGG 2 cut(s) 936, 1167
PspLI CGTACG 1 cut(s) 820
PspPI GGNCC 3 cut(s) 60, 292, 5155
PspXI VCTCGAGB 1 cut(s) 1004
PsrI GAACNNNNNNTAC 2 cut(s) 342, 374
PstI CTGCAG 5 cut(s) 1267, 1642, 2182, 3103, 3961
PstNI CAGNNNCTG 6 cut(s) 2555, 2765, 3476, 3686, 4334, 4544
PsuI RGATCY 9 cut(s) 496, 1724, 2264, 2734, 3185, 3655, 4043, 4513, 5506
RseI CAYNNNNRTG 4 cut(s) 449, 516, 4703, 4845
SacI GAGCTC 1 cut(s) 1291
SalI GTCGAC 1 cut(s) 161
Sau96I GGNCC 3 cut(s) 60, 292, 5155
ScrFI CCNGG 2 cut(s) 938, 1169
SduI GDGCHC 6 cut(s) 36, 538, 1291, 2655, 3576, 4434
SfaNI GCATC 9 cut(s) 1087, 1366, 2860, 3781, 4639, 4816, 4947, 5295, 5333
SfoI GGCGCC 1 cut(s) 19
Sfr274I CTCGAG 2 cut(s) 167, 1004
SinI GGWCC 2 cut(s) 60, 5155
SlaI CTCGAG 2 cut(s) 167, 1004
SmiMI CAYNNNNRTG 4 cut(s) 449, 516, 4703, 4845
SmlI CTYRAG 4 cut(s) 167, 397, 569, 1004
SmoI CTYRAG 4 cut(s) 167, 397, 569, 1004
SphI GCATGC 2 cut(s) 1325, 4844
SspDI GGCGCC 1 cut(s) 17
SspI AATATT 3 cut(s) 2842, 3763, 4621
SstI GAGCTC 1 cut(s) 1291
StyD4I CCNGG 2 cut(s) 936, 1167
StyI CCWWGG 8 cut(s) 285, 687, 872, 1058, 2775, 3696, 4554, 4683
TaiI ACGT 9 cut(s) 11, 51, 742, 1057, 1252, 1918, 2458, 3379, 4237
TatI WGTACW 8 cut(s) 1234, 1796, 2336, 2611, 3257, 3532, 4115, 4390
TauI GCSGC 3 cut(s) 215, 4760, 5324
TseFI GTSAC 5 cut(s) 256, 932, 4744, 4935, 5370
Tsp45I GTSAC 5 cut(s) 256, 932, 4744, 4935, 5370
TspGWI ACGGA 3 cut(s) 42, 1359, 5062
Van91I CCANNNNNTGG 1 cut(s) 253
VpaK11BI GGWCC 2 cut(s) 60, 5155
VspI ATTAAT 4 cut(s) 1929, 2469, 3390, 4248
XagI CCTNNNNNAGG 4 cut(s) 1419, 2774, 3695, 4553
XbaI TCTAGA 1 cut(s) 5338
XceI RCATGY 8 cut(s) 1078, 1325, 2756, 3677, 4535, 4820, 4840, 4844
XhoI CTCGAG 2 cut(s) 167, 1004
XmaJI CCTAGG 1 cut(s) 872
XmiI GTMKAC 1 cut(s) 162
XmnI GAANNNNTTC 5 cut(s) 318, 1188, 2514, 3435, 4293
Zsp2I ATGCAT 1 cut(s) 4842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.