Rh6AG332000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0005240
Physical Location & Seq
Forward (+)
53334300 .. 53338835
4536 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG332000.1

Sequence Viewer

Length: 1428 bp
ATGGGGTTTAATGCGGTTTTTAGCTCCTTGAAGGAGGTTTTTCCTCAGGTTGATTTTCGTGTTCTGAAAGCTGTTGCTATAGAAAATTCTAATGATCTTGACGCAGCTGTCAATGATGTGCTGAATGAGGTTCTCCCCTTCTTGACCAAACATCCTGAGAGTCCTGCAAAGGTTCAGACTCCTAGCAGCCAACCAACTGCAGTTGAACCTGAGGAGCAGAGTAAAGAATTAAATCACCGGCAGGTAGTTGAGGAAGTAGAGGTTGAGTCTTTTCCAGCAGCAAGATCCACTGATGTTGACAATGCCATCACTACTGATCAAACAGAATTTACAAGTGGTGCCTCCCATGAGGTTTGTACACTGGTAGATGACACAAATTCATCACAGAATCTCCTTGCCTCTTCAGATGGAGATCAAGCAACTGGCCATGATGAAAAAGAATTTTTGAACACTGGAAGTGAAGAACTGGAGCGACGTGGAAAGAAGATTAATGAGGATGATGACACTTTCATATTGCCAGAGGGGGTAGAAACATTTCTGAGTGATACTCGGCTTTATACCGAAACAACAGCAGCTGGTAATGTCGAAGCTGGGCTTCAGCAGTCTTCATCTGGAATGGCAACCAGTTCAGTACATGAAAAAGAGGGGGTTCATGACGGCTTGGTCAATGTGCCCTATGAATGGAAGGATTTTGATTTTTCTCAGACTGATGATCTGTCTCTGTTGGAGGGTGAAAGTTCTATTGTTCAGCTGGATCCTCCTTTTCCAGAACATGCAACAGAAACTGTCCTTTCCAAGGAAGACGAGTCTTTCTGTAATATGGCTGATATGAAGGAGTCCACCACGAATAGCATATGCAATATTGATGTTCTCGAAGAGAGCATTGAAGATGCTAAGCATAACAAGAGAACATTGTTTTCAGCCATGGAGTCTGTTATCACCATGATGAGAGAAGTGGAACTGGAAGAGAAAGCTGTGGATCGTGTCACAGAGGAAGCCGCAAGGGGAGGTCAGGATATTATGGTTAAGGTGGAGGAACTAAAACTGATGTTGGCACATGCGAAGGATGCAAATGACATGCATGCTGGTGAAGTTTATGGGGAGAAGGCAATTTTAGCAACTGAAGTTAGAGAGCTTCAATGTCGATTGCTCAGTTTGTCAGATGAAAGGGATAAGTCACTTGCAATCCTTGACGAGATGCGTGAAACACTTGAAGCACGACTAACTGGAGTTGCAGAGTTGAGAAAAATGGCCGAGGATGAAAAGCTAGAAAAGGAAGAATCTGCACGGAAAGCTCTTGCTGAACAAGAAGCCATCATGGAGAAAGTGGTTGAGGAGTCCAAGACACTGCAACAGGCAGCGGAGGAAAATTCCAAGTTGCGGGAGTTTCTGATGGACCGTGGCCACATTGTAGATATGCTACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CUE

Protein Analysis

475

Amino Acids

52.81

Weight (kDa)

4.4

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CUE PF02845 5 - 42 4.4e-06 CUE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 107, 662
Acc36I ACCTGC 1 cut(s) 232
AccB1I GGYRCC 1 cut(s) 338
AciI CCGC 4 cut(s) 14, 999, 1361, 1381
AclWI GGATC 4 cut(s) 279, 749, 762, 987
AcoI YGGCCR 3 cut(s) 424, 1251, 1402
AcsI RAATTY 5 cut(s) 85, 326, 376, 440, 1369
AcuI CTGAAG 3 cut(s) 387, 581, 1143
AfaI GTAC 2 cut(s) 358, 633
AfiI CCNNNNNNNGG 3 cut(s) 681, 796, 1380
AgsI TTSAA 6 cut(s) 31, 206, 448, 887, 1139, 1214
AjiI CACGTC 1 cut(s) 476
Alw26I GTCTC 1 cut(s) 723
AlwI GGATC 4 cut(s) 279, 749, 762, 987
AlwNI CAGNNNCTG 2 cut(s) 575, 785
AoxI GGCC 3 cut(s) 424, 1251, 1402
ApeKI GCWGC 5 cut(s) 104, 186, 278, 572, 1358
ApoI RAATTY 5 cut(s) 85, 326, 376, 440, 1369
AseI ATTAAT 1 cut(s) 489
Asp700I GAANNNNTTC 1 cut(s) 534
AspS9I GGNCC 1 cut(s) 1396
AsuHPI GGTGA 4 cut(s) 227, 743, 931, 1100
AvaII GGWCC 1 cut(s) 1396
AxyI CCTNAGG 2 cut(s) 45, 210
BaeGI GKGCMC 1 cut(s) 675
BalI TGGCCA 2 cut(s) 426, 1404
BamHI GGATCC 1 cut(s) 754
BanI GGYRCC 1 cut(s) 338
BbsI GAAGAC 2 cut(s) 597, 807
BbvI GCAGC 5 cut(s) 116, 198, 290, 584, 1370
BccI CCATC 4 cut(s) 314, 401, 1322, 1387
BceAI ACGGC 1 cut(s) 673
BclI TGATCA 1 cut(s) 316
BcoDI GTCTC 1 cut(s) 723
BfaI CTAG 2 cut(s) 183, 1268
BfmI CTRYAG 3 cut(s) 78, 198, 1421
BfuAI ACCTGC 1 cut(s) 232
BisI GCNGC 6 cut(s) 105, 187, 279, 573, 999, 1359
BlpI GCTNAGC 1 cut(s) 894
BlsI GCNGC 6 cut(s) 106, 188, 280, 574, 1000, 1360
Bme18I GGWCC 1 cut(s) 1396
BmgBI CACGTC 1 cut(s) 476
BmgT120I GGNCC 1 cut(s) 1396
BmiI GGNNCC 2 cut(s) 340, 756
BmsI GCATC 3 cut(s) 880, 1057, 1188
BpiI GAAGAC 2 cut(s) 597, 807
BplI GAGNNNNNCTC 2 cut(s) 532, 564
BpmI CTGGAG 2 cut(s) 488, 1248
Bpu1102I GCTNAGC 1 cut(s) 894
BsaBI GATNNNNATC 1 cut(s) 411
BsaJI CCNNGG 4 cut(s) 795, 924, 1254, 1399
BsaXI ACNNNNNCTCC 2 cut(s) 375, 405
Bsc4I CCNNNNNNNGG 3 cut(s) 681, 796, 1380
Bse118I RCCGGY 1 cut(s) 237
Bse1I ACTGG 7 cut(s) 366, 427, 457, 471, 624, 966, 1231
Bse21I CCTNAGG 2 cut(s) 45, 210
Bse8I GATNNNNATC 1 cut(s) 411
BseDI CCNNGG 4 cut(s) 795, 924, 1254, 1399
BseGI GGATG 4 cut(s) 151, 502, 1072, 1264
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 3 cut(s) 681, 796, 1380
BseMII CTCAG 6 cut(s) 59, 147, 201, 530, 716, 1165
BseNI ACTGG 7 cut(s) 366, 427, 457, 471, 624, 966, 1231
BseRI GAGGAG 2 cut(s) 227, 1349
BseSI GKGCMC 1 cut(s) 675
BseXI GCAGC 5 cut(s) 116, 198, 290, 584, 1370
BseYI CCCAGC 1 cut(s) 590
BsgI GTGCAG 1 cut(s) 1269
BshFI GGCC 3 cut(s) 426, 1253, 1404
BshNI GGYRCC 1 cut(s) 338
BsiSI CCGG 1 cut(s) 238
BslI CCNNNNNNNGG 3 cut(s) 681, 796, 1380
BsmAI GTCTC 1 cut(s) 723
BsnI GGCC 3 cut(s) 426, 1253, 1404
Bsp1286I GDGCHC 1 cut(s) 675
Bsp1407I TGTACA 1 cut(s) 356
Bsp143I GATC 7 cut(s) 94, 284, 316, 412, 712, 754, 979
Bsp1720I GCTNAGC 1 cut(s) 894
Bsp19I CCATGG 1 cut(s) 924
BspACI CCGC 4 cut(s) 14, 999, 1361, 1381
BspANI GGCC 3 cut(s) 426, 1253, 1404
BspCNI CTCAG 6 cut(s) 58, 148, 202, 531, 715, 1164
BspHI TCATGA 1 cut(s) 652
BspLI GGNNCC 2 cut(s) 340, 756
BspMAI CTGCAG 1 cut(s) 202
BspMI ACCTGC 1 cut(s) 232
BspPI GGATC 4 cut(s) 279, 749, 762, 987
BspT107I GGYRCC 1 cut(s) 338
BsrFI RCCGGY 1 cut(s) 237
BsrGI TGTACA 1 cut(s) 356
BsrI ACTGG 7 cut(s) 366, 427, 457, 471, 624, 966, 1231
BssAI RCCGGY 1 cut(s) 237
BssECI CCNNGG 4 cut(s) 795, 924, 1254, 1399
BssMI GATC 7 cut(s) 94, 284, 316, 412, 712, 754, 979
BssT1I CCWWGG 2 cut(s) 795, 924
Bst4CI ACNGT 3 cut(s) 787, 1400, 1425
Bst6I CTCTTC 3 cut(s) 406, 870, 960
BstAUI TGTACA 1 cut(s) 356
BstC8I GCNNGC 1 cut(s) 1083
BstDEI CTNAG 7 cut(s) 45, 156, 210, 539, 702, 894, 1151
BstDSI CCRYGG 2 cut(s) 924, 1399
BstENI CCTNNNNNAGG 1 cut(s) 794
BstF5I GGATG 4 cut(s) 151, 502, 1072, 1264
BstKTI GATC 7 cut(s) 97, 287, 319, 415, 715, 757, 982
BstMAI GTCTC 1 cut(s) 723
BstMBI GATC 7 cut(s) 94, 284, 316, 412, 712, 754, 979
BstMWI GCNNNNNNNGC 3 cut(s) 1067, 1115, 1292
BstNSI RCATGY 4 cut(s) 776, 1061, 1081, 1085
BstSFI CTRYAG 3 cut(s) 78, 198, 1421
BstSLI GKGCMC 1 cut(s) 675
BstV1I GCAGC 5 cut(s) 116, 198, 290, 584, 1370
BstV2I GAAGAC 2 cut(s) 597, 807
BstX2I RGATCY 2 cut(s) 284, 754
BstYI RGATCY 2 cut(s) 284, 754
Bsu36I CCTNAGG 2 cut(s) 45, 210
BsuRI GGCC 3 cut(s) 426, 1253, 1404
BtgI CCRYGG 2 cut(s) 924, 1399
BtrI CACGTC 1 cut(s) 476
BtsCI GGATG 4 cut(s) 151, 502, 1072, 1264
BtsI GCAGTG 1 cut(s) 1346
BtsIMutI CAGTG 4 cut(s) 288, 359, 450, 1346
BveI ACCTGC 1 cut(s) 232
Cac8I GCNNGC 1 cut(s) 1083
CaiI CAGNNNCTG 2 cut(s) 575, 785
CciI TCATGA 1 cut(s) 652
Cfr10I RCCGGY 1 cut(s) 237
Cfr13I GGNCC 1 cut(s) 1396
CseI GACGC 1 cut(s) 110
Csp6I GTAC 2 cut(s) 357, 632
CspCI CAANNNNNGTGG 2 cut(s) 277, 312
CviQI GTAC 2 cut(s) 357, 632
DdeI CTNAG 7 cut(s) 45, 156, 210, 539, 702, 894, 1151
DpnI GATC 7 cut(s) 96, 286, 318, 414, 714, 756, 981
DpnII GATC 7 cut(s) 94, 284, 316, 412, 712, 754, 979
DrdI GACNNNNNNGTC 2 cut(s) 107, 662
DseDI GACNNNNNNGTC 2 cut(s) 107, 662
EaeI YGGCCR 3 cut(s) 424, 1251, 1402
Eam1104I CTCTTC 3 cut(s) 406, 870, 960
EarI CTCTTC 3 cut(s) 406, 870, 960
Eco130I CCWWGG 2 cut(s) 795, 924
Eco47I GGWCC 1 cut(s) 1396
Eco57I CTGAAG 3 cut(s) 387, 581, 1143
Eco81I CCTNAGG 2 cut(s) 45, 210
EcoNI CCTNNNNNAGG 1 cut(s) 794
EcoT14I CCWWGG 2 cut(s) 795, 924
EcoT22I ATGCAT 1 cut(s) 1083
ErhI CCWWGG 2 cut(s) 795, 924
FalI AAGNNNNNCTT 2 cut(s) 579, 611
FauI CCCGC 1 cut(s) 1374
FauNDI CATATG 1 cut(s) 854
FbaI TGATCA 1 cut(s) 316
Fnu4HI GCNGC 6 cut(s) 105, 187, 279, 573, 999, 1359
FokI GGATG 4 cut(s) 138, 509, 1079, 1271
Fsp4HI GCNGC 6 cut(s) 105, 187, 279, 573, 999, 1359
FspBI CTAG 2 cut(s) 183, 1268
GluI GCNGC 6 cut(s) 105, 187, 279, 573, 999, 1359
GsaI CCCAGC 1 cut(s) 594
GsuI CTGGAG 2 cut(s) 488, 1248
HaeIII GGCC 3 cut(s) 426, 1253, 1404
HapII CCGG 1 cut(s) 238
HgaI GACGC 1 cut(s) 110
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HinfI GANTC 9 cut(s) 160, 178, 266, 388, 806, 836, 929, 1280, 1337
HpaII CCGG 1 cut(s) 238
HphI GGTGA 4 cut(s) 227, 743, 931, 1100
Hpy166II GTNNAC 3 cut(s) 298, 359, 840
Hpy188I TCNGA 7 cut(s) 66, 177, 406, 540, 705, 1162, 1392
Hpy188III TCNNGA 8 cut(s) 98, 142, 155, 612, 653, 767, 872, 1013
Hpy8I GTNNAC 3 cut(s) 298, 359, 840
Hpy99I CGWCG 1 cut(s) 477
HpyAV CCTTC 6 cut(s) 25, 148, 679, 826, 1057, 1099
HpyCH4III ACNGT 3 cut(s) 787, 1400, 1425
HpyCH4IV ACGT 1 cut(s) 475
HpyF10VI GCNNNNNNNGC 3 cut(s) 1067, 1115, 1292
HpyF3I CTNAG 7 cut(s) 45, 156, 210, 539, 702, 894, 1151
HpySE526I ACGT 1 cut(s) 475
Ksp22I TGATCA 1 cut(s) 316
Kzo9I GATC 7 cut(s) 94, 284, 316, 412, 712, 754, 979
LmnI GCTCC 3 cut(s) 29, 214, 469
Lsp1109I GCAGC 5 cut(s) 116, 198, 290, 584, 1370
LweI GCATC 3 cut(s) 880, 1057, 1188
MaeI CTAG 2 cut(s) 183, 1268
MaeII ACGT 1 cut(s) 475
MaeIII GTNAC 2 cut(s) 985, 1176
MalI GATC 7 cut(s) 96, 286, 318, 414, 714, 756, 981
MboI GATC 7 cut(s) 94, 284, 316, 412, 712, 754, 979
MboII GAAGA 9 cut(s) 393, 473, 496, 597, 812, 887, 899, 977, 1289
MflI RGATCY 2 cut(s) 284, 754
MhlI GDGCHC 1 cut(s) 675
MlsI TGGCCA 2 cut(s) 426, 1404
MluCI AATT 7 cut(s) 85, 227, 326, 376, 440, 1110, 1369
MluNI TGGCCA 2 cut(s) 426, 1404
MlyI GAGTC 7 cut(s) 169, 172, 275, 815, 845, 938, 1346
MmeI TCCRAC 1 cut(s) 705
Mox20I TGGCCA 2 cut(s) 426, 1404
Mph1103I ATGCAT 1 cut(s) 1083
MroXI GAANNNNTTC 1 cut(s) 534
MscI TGGCCA 2 cut(s) 426, 1404
MseI TTAA 4 cut(s) 9, 230, 489, 1026
MslI CAYNNNNRTG 2 cut(s) 944, 1086
Msp20I TGGCCA 2 cut(s) 426, 1404
MspA1I CMGCKG 4 cut(s) 107, 575, 751, 1361
MspI CCGG 1 cut(s) 238
MwoI GCNNNNNNNGC 3 cut(s) 1067, 1115, 1292
NcoI CCATGG 1 cut(s) 924
NdeI CATATG 1 cut(s) 854
NdeII GATC 7 cut(s) 94, 284, 316, 412, 712, 754, 979
NlaIV GGNNCC 2 cut(s) 340, 756
NmeAIII GCCGAG 2 cut(s) 529, 1279
NmuCI GTSAC 2 cut(s) 985, 1176
NsiI ATGCAT 1 cut(s) 1083
NspI RCATGY 4 cut(s) 776, 1061, 1081, 1085
PaeI GCATGC 1 cut(s) 1085
PagI TCATGA 1 cut(s) 652
PdmI GAANNNNTTC 1 cut(s) 534
PfeI GAWTC 2 cut(s) 388, 1280
PkrI GCNGC 6 cut(s) 106, 188, 280, 574, 1000, 1360
PleI GAGTC 7 cut(s) 168, 172, 274, 814, 844, 937, 1345
PpsI GAGTC 7 cut(s) 168, 172, 274, 814, 844, 937, 1345
PshBI ATTAAT 1 cut(s) 489
PspFI CCCAGC 1 cut(s) 590
PspN4I GGNNCC 2 cut(s) 340, 756
PspPI GGNCC 1 cut(s) 1396
PstI CTGCAG 1 cut(s) 202
PstNI CAGNNNCTG 2 cut(s) 575, 785
PsuI RGATCY 2 cut(s) 284, 754
PvuII CAGCTG 3 cut(s) 107, 575, 751
RsaI GTAC 2 cut(s) 358, 633
RsaNI GTAC 2 cut(s) 357, 632
RseI CAYNNNNRTG 2 cut(s) 944, 1086
SaqAI TTAA 4 cut(s) 9, 230, 489, 1026
SatI GCNGC 6 cut(s) 105, 187, 279, 573, 999, 1359
Sau3AI GATC 7 cut(s) 94, 284, 316, 412, 712, 754, 979
Sau96I GGNCC 1 cut(s) 1396
SchI GAGTC 7 cut(s) 169, 172, 275, 815, 845, 938, 1346
SduI GDGCHC 1 cut(s) 675
SfaNI GCATC 3 cut(s) 880, 1057, 1188
SfcI CTRYAG 3 cut(s) 78, 198, 1421
SinI GGWCC 1 cut(s) 1396
SmiMI CAYNNNNRTG 2 cut(s) 944, 1086
SphI GCATGC 1 cut(s) 1085
Sse9I AATT 7 cut(s) 85, 227, 326, 376, 440, 1110, 1369
SsiI CCGC 4 cut(s) 14, 999, 1361, 1381
SspI AATATT 1 cut(s) 862
SspMI CTAG 2 cut(s) 183, 1268
StyI CCWWGG 2 cut(s) 795, 924
TaaI ACNGT 3 cut(s) 787, 1400, 1425
TaiI ACGT 1 cut(s) 478
TaqI TCGA 3 cut(s) 585, 873, 1144
TasI AATT 7 cut(s) 85, 227, 326, 376, 440, 1110, 1369
TatI WGTACW 2 cut(s) 356, 631
TauI GCSGC 1 cut(s) 1001
TfiI GAWTC 2 cut(s) 388, 1280
Tru1I TTAA 4 cut(s) 9, 230, 489, 1026
Tru9I TTAA 4 cut(s) 9, 230, 489, 1026
TscAI CASTG 4 cut(s) 295, 366, 457, 1353
TseFI GTSAC 2 cut(s) 985, 1176
TseI GCWGC 5 cut(s) 104, 186, 278, 572, 1358
Tsp45I GTSAC 2 cut(s) 985, 1176
TspGWI ACGGA 1 cut(s) 1303
TspRI CASTG 4 cut(s) 295, 366, 457, 1353
VpaK11BI GGWCC 1 cut(s) 1396
VspI ATTAAT 1 cut(s) 489
XagI CCTNNNNNAGG 1 cut(s) 794
XapI RAATTY 5 cut(s) 85, 326, 376, 440, 1369
XceI RCATGY 4 cut(s) 776, 1061, 1081, 1085
XmnI GAANNNNTTC 1 cut(s) 534
XspI CTAG 2 cut(s) 183, 1268
Zsp2I ATGCAT 1 cut(s) 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.