Rw6G028840

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0005240
Physical Location & Seq
Forward (+)
52891912 .. 52895647
3736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G028840.1

Sequence Viewer

Length: 1716 bp
ATGGGGTTTAATGCGGTTTTTAGCTCCTTGAAGGAGGTTTTTCCTCAGGTTGATTTTCGTGTTCTGAAAGCTGTTGCTATAGAAAATTCTAATGATCTTGACGCAGCTGTCAATGATGTGCTGAATGAGGTTCTCCCCTTCTTGACCAAACATCCTGAGAGTCCTGCAAAGGTTCAGACTCCTAGCAGCCAACCAACTGCAGTTGAGGAAGTAGAGGTTGAGTCTTTTCCAGCAGCAAGATCCACTGATGTTGACAATGCCATCACTACTGATCAAACAGAATTTACAAGTGGTGCCTCCCATGAGGTTTGTACACTGGTAGATGACACAAATTCATCACAGAATCTCCTTGCCTCTTCAGATGGAGATCAAGCAACTGGCCATGATGAAAAAGAATTTTTGAACACTGGAAGTGAAGAACTGGAGCGACGTGGAAAGAAGATTAATGAGGATGATGACACTTTCATATTGCCAGAGGGGGTAGAAACATTTCTGAGTGATACTCGGCTTTATACCGAAACAACAGCAGCTGGTAATGTCGAAGCTGGGCTTCAGCAGTCTTCATCTGGAATGGCAACCAGTTCAGTACATGAAAAAGAGGGGGTTCATGACGGCTTGGTCAATGTGCCCTATGAATGGAAGGATTTTGATTTTTCTCAGACTGATGATCTGTCTCTGTTGGAGGGTGAAAGTTCTATTGTTCAGCTGGATCCTCCTTTTCCAGAACATGCAACAGAAACTGTCCTTTCCAAGGAAGACGAGTCTTTCTGTAATATGGCTGATATGAAGGAGTCCACCACGAATAGCATATGCAATATTGATGTTCTCGAAGAGAGCATTGAAGATGCTAAGCATAACAAGAGAACATTGTTTTCAGCCATGGAGTCTGTTATCACCATGATGAGAGAAGTGGAACTGGAAGAGAAAGCTGTGGATCGTGTCACAGAGGAAGCCGCAAGGGGAGGTCAGGATATTATGGTTAAGGTGGAGGAACTAAAACTGATGTTGGCACATGCGAAGGATGCAAATGACATGCATGCTGGTGAAGTTTATGGGGAGAAGGCAATTTTAGCAACTGAAGTTAGAGAGCTTCAATGTCGATTGCTCAGTTTGTCAGATGAAAGGGATAAGTCACTTGCAATCCTTGACGAGATGCGTGAAACACTTGAAGCACGACTAACTGGAGTTGCAGAGTTGAGAAAAATGGCCGAGGATGAAAAGCTAGAAAAGGAAGAATCTGCACGGAAAGCTCTTGCTGAACAAGAAGCCATCATGGAGAAAGTGGTTGAGGAGTCCAAGACACTGCAACAGGCAGCGGAGGAAAATTCCAAGTTGCGGGAGTTTCTGATGGACCGTGGCCACATTGTAGATATGCTACAAGGAGAAATCTCTGTAATTTGTCAGGATGTCAAGTTGCTGAAAGAGAAGTTTGATGATCGTGTTCCTCTAAGCCAGTCTGTTTCATCTAGCCAGACAAGTTGCATCTTAGCTTCTTCAGGCTCATCCTTGAAAAGCGCGGCATCTGACTTGCTTCTAGAACGAGTTGGGTCAGTTGGGTCATCCGAGTCACCAAAGACCGTAGAAAGGGCAAGTCCTGTTGCTTCAGTTGACGGTCTCTCACCAAAATACAGACTAGAAGATAAAACAGTCAAGGTCGATAAACAAGCTCTTATGGCCGATGGGTGGGATGTCTTTGACGAAGATCTCTTGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CUE

Protein Analysis

571

Amino Acids

62.85

Weight (kDa)

4.42

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CUE PF02845 5 - 42 5.5e-06 CUE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 107, 617
AccB1I GGYRCC 1 cut(s) 293
AccII CGCG 1 cut(s) 1517
AciI CCGC 5 cut(s) 14, 954, 1316, 1336, 1517
AclWI GGATC 4 cut(s) 234, 704, 717, 942
AcoI YGGCCR 4 cut(s) 379, 1206, 1357, 1674
AcsI RAATTY 5 cut(s) 85, 281, 331, 395, 1324
AcuI CTGAAG 5 cut(s) 342, 536, 1098, 1479, 1587
AfaI GTAC 2 cut(s) 313, 588
AfiI CCNNNNNNNGG 5 cut(s) 636, 751, 1335, 1584, 1683
AgsI TTSAA 6 cut(s) 31, 403, 842, 1094, 1169, 1510
AjiI CACGTC 1 cut(s) 431
Alw26I GTCTC 2 cut(s) 678, 1619
AlwI GGATC 4 cut(s) 234, 704, 717, 942
AlwNI CAGNNNCTG 2 cut(s) 530, 740
AoxI GGCC 4 cut(s) 379, 1206, 1357, 1674
ApeKI GCWGC 5 cut(s) 104, 186, 233, 527, 1313
ApoI RAATTY 5 cut(s) 85, 281, 331, 395, 1324
AseI ATTAAT 1 cut(s) 444
Asp700I GAANNNNTTC 1 cut(s) 489
AspLEI GCGC 1 cut(s) 1517
AspS9I GGNCC 1 cut(s) 1351
AsuHPI GGTGA 5 cut(s) 698, 886, 1055, 1560, 1611
AvaII GGWCC 1 cut(s) 1351
AxyI CCTNAGG 1 cut(s) 45
BaeGI GKGCMC 1 cut(s) 630
BalI TGGCCA 2 cut(s) 381, 1359
BamHI GGATCC 1 cut(s) 709
BanI GGYRCC 1 cut(s) 293
BbsI GAAGAC 2 cut(s) 552, 762
BbvI GCAGC 5 cut(s) 116, 198, 245, 539, 1325
BccI CCATC 5 cut(s) 269, 356, 1277, 1342, 1673
BceAI ACGGC 1 cut(s) 628
BclI TGATCA 1 cut(s) 271
BcoDI GTCTC 2 cut(s) 678, 1619
BfaI CTAG 5 cut(s) 183, 1223, 1467, 1535, 1634
BfmI CTRYAG 2 cut(s) 78, 198
BglII AGATCT 1 cut(s) 1702
BisI GCNGC 7 cut(s) 105, 187, 234, 528, 954, 1314, 1518
BlpI GCTNAGC 1 cut(s) 849
BlsI GCNGC 7 cut(s) 106, 188, 235, 529, 955, 1315, 1519
Bme18I GGWCC 1 cut(s) 1351
BmgBI CACGTC 1 cut(s) 431
BmgT120I GGNCC 1 cut(s) 1351
BmiI GGNNCC 2 cut(s) 295, 711
BmsI GCATC 5 cut(s) 835, 1012, 1143, 1491, 1529
BpiI GAAGAC 2 cut(s) 552, 762
BplI GAGNNNNNCTC 2 cut(s) 487, 519
BpmI CTGGAG 2 cut(s) 443, 1203
Bpu1102I GCTNAGC 1 cut(s) 849
BsaBI GATNNNNATC 1 cut(s) 366
BsaI GGTCTC 1 cut(s) 1619
BsaJI CCNNGG 4 cut(s) 750, 879, 1209, 1354
BsaXI ACNNNNNCTCC 2 cut(s) 330, 360
Bsc4I CCNNNNNNNGG 5 cut(s) 636, 751, 1335, 1584, 1683
Bse1I ACTGG 8 cut(s) 321, 382, 412, 426, 579, 921, 1186, 1453
Bse21I CCTNAGG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 366
BseDI CCNNGG 4 cut(s) 750, 879, 1209, 1354
BseGI GGATG 8 cut(s) 151, 457, 1027, 1219, 1411, 1502, 1559, 1693
BseJI GATNNNNATC 1 cut(s) 366
BseLI CCNNNNNNNGG 5 cut(s) 636, 751, 1335, 1584, 1683
BseMII CTCAG 5 cut(s) 59, 147, 485, 671, 1120
BseNI ACTGG 8 cut(s) 321, 382, 412, 426, 579, 921, 1186, 1453
BseRI GAGGAG 1 cut(s) 1304
BseSI GKGCMC 1 cut(s) 630
BseXI GCAGC 5 cut(s) 116, 198, 245, 539, 1325
BseYI CCCAGC 1 cut(s) 545
BsgI GTGCAG 1 cut(s) 1224
Bsh1236I CGCG 1 cut(s) 1517
BshFI GGCC 4 cut(s) 381, 1208, 1359, 1676
BshNI GGYRCC 1 cut(s) 293
BslI CCNNNNNNNGG 5 cut(s) 636, 751, 1335, 1584, 1683
BsmAI GTCTC 2 cut(s) 678, 1619
BsnI GGCC 4 cut(s) 381, 1208, 1359, 1676
Bso31I GGTCTC 1 cut(s) 1619
Bsp1286I GDGCHC 1 cut(s) 630
Bsp1407I TGTACA 1 cut(s) 311
Bsp143I GATC 9 cut(s) 94, 239, 271, 367, 667, 709, 934, 1435, 1702
Bsp1720I GCTNAGC 1 cut(s) 849
Bsp19I CCATGG 1 cut(s) 879
BspACI CCGC 5 cut(s) 14, 954, 1316, 1336, 1517
BspANI GGCC 4 cut(s) 381, 1208, 1359, 1676
BspCNI CTCAG 5 cut(s) 58, 148, 486, 670, 1119
BspFNI CGCG 1 cut(s) 1517
BspHI TCATGA 1 cut(s) 607
BspLI GGNNCC 2 cut(s) 295, 711
BspMAI CTGCAG 1 cut(s) 202
BspPI GGATC 4 cut(s) 234, 704, 717, 942
BspT107I GGYRCC 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 1619
BsrGI TGTACA 1 cut(s) 311
BsrI ACTGG 8 cut(s) 321, 382, 412, 426, 579, 921, 1186, 1453
BssECI CCNNGG 4 cut(s) 750, 879, 1209, 1354
BssMI GATC 9 cut(s) 94, 239, 271, 367, 667, 709, 934, 1435, 1702
BssT1I CCWWGG 2 cut(s) 750, 879
Bst4CI ACNGT 5 cut(s) 742, 1355, 1579, 1613, 1648
Bst6I CTCTTC 3 cut(s) 361, 825, 915
BstAUI TGTACA 1 cut(s) 311
BstC8I GCNNGC 1 cut(s) 1038
BstDEI CTNAG 8 cut(s) 45, 156, 494, 657, 849, 1106, 1448, 1486
BstDSI CCRYGG 2 cut(s) 879, 1354
BstENI CCTNNNNNAGG 1 cut(s) 749
BstF5I GGATG 8 cut(s) 151, 457, 1027, 1219, 1411, 1502, 1559, 1693
BstFNI CGCG 1 cut(s) 1517
BstHHI GCGC 1 cut(s) 1517
BstKTI GATC 9 cut(s) 97, 242, 274, 370, 670, 712, 937, 1438, 1705
BstMAI GTCTC 2 cut(s) 678, 1619
BstMBI GATC 9 cut(s) 94, 239, 271, 367, 667, 709, 934, 1435, 1702
BstMWI GCNNNNNNNGC 4 cut(s) 1022, 1070, 1247, 1673
BstNSI RCATGY 4 cut(s) 731, 1016, 1036, 1040
BstSFI CTRYAG 2 cut(s) 78, 198
BstSLI GKGCMC 1 cut(s) 630
BstUI CGCG 1 cut(s) 1517
BstV1I GCAGC 5 cut(s) 116, 198, 245, 539, 1325
BstV2I GAAGAC 2 cut(s) 552, 762
BstX2I RGATCY 3 cut(s) 239, 709, 1702
BstYI RGATCY 3 cut(s) 239, 709, 1702
Bsu36I CCTNAGG 1 cut(s) 45
BsuRI GGCC 4 cut(s) 381, 1208, 1359, 1676
BtgI CCRYGG 2 cut(s) 879, 1354
BtrI CACGTC 1 cut(s) 431
BtsCI GGATG 8 cut(s) 151, 457, 1027, 1219, 1411, 1502, 1559, 1693
BtsI GCAGTG 1 cut(s) 1301
BtsIMutI CAGTG 4 cut(s) 243, 314, 405, 1301
Cac8I GCNNGC 1 cut(s) 1038
CaiI CAGNNNCTG 2 cut(s) 530, 740
CciI TCATGA 1 cut(s) 607
CfoI GCGC 1 cut(s) 1517
Cfr13I GGNCC 1 cut(s) 1351
CseI GACGC 1 cut(s) 110
Csp6I GTAC 2 cut(s) 312, 587
CspCI CAANNNNNGTGG 2 cut(s) 232, 267
CviQI GTAC 2 cut(s) 312, 587
DdeI CTNAG 8 cut(s) 45, 156, 494, 657, 849, 1106, 1448, 1486
DpnI GATC 9 cut(s) 96, 241, 273, 369, 669, 711, 936, 1437, 1704
DpnII GATC 9 cut(s) 94, 239, 271, 367, 667, 709, 934, 1435, 1702
DrdI GACNNNNNNGTC 2 cut(s) 107, 617
DseDI GACNNNNNNGTC 2 cut(s) 107, 617
EaeI YGGCCR 4 cut(s) 379, 1206, 1357, 1674
Eam1104I CTCTTC 3 cut(s) 361, 825, 915
EarI CTCTTC 3 cut(s) 361, 825, 915
Eco130I CCWWGG 2 cut(s) 750, 879
Eco31I GGTCTC 1 cut(s) 1619
Eco47I GGWCC 1 cut(s) 1351
Eco57I CTGAAG 5 cut(s) 342, 536, 1098, 1479, 1587
Eco81I CCTNAGG 1 cut(s) 45
EcoNI CCTNNNNNAGG 1 cut(s) 749
EcoT14I CCWWGG 2 cut(s) 750, 879
EcoT22I ATGCAT 1 cut(s) 1038
ErhI CCWWGG 2 cut(s) 750, 879
FalI AAGNNNNNCTT 2 cut(s) 534, 566
FauI CCCGC 1 cut(s) 1329
FauNDI CATATG 1 cut(s) 809
FbaI TGATCA 1 cut(s) 271
Fnu4HI GCNGC 7 cut(s) 105, 187, 234, 528, 954, 1314, 1518
FokI GGATG 8 cut(s) 138, 464, 1034, 1226, 1418, 1489, 1546, 1700
Fsp4HI GCNGC 7 cut(s) 105, 187, 234, 528, 954, 1314, 1518
FspBI CTAG 5 cut(s) 183, 1223, 1467, 1535, 1634
GlaI GCGC 1 cut(s) 1516
GluI GCNGC 7 cut(s) 105, 187, 234, 528, 954, 1314, 1518
GsaI CCCAGC 1 cut(s) 549
GsuI CTGGAG 2 cut(s) 443, 1203
HaeIII GGCC 4 cut(s) 381, 1208, 1359, 1676
HgaI GACGC 1 cut(s) 110
HhaI GCGC 1 cut(s) 1517
Hin6I GCGC 1 cut(s) 1515
HinP1I GCGC 1 cut(s) 1515
HincII GTYRAC 2 cut(s) 253, 1609
HindII GTYRAC 2 cut(s) 253, 1609
HphI GGTGA 5 cut(s) 698, 886, 1055, 1560, 1611
Hpy166II GTNNAC 4 cut(s) 253, 314, 795, 1609
Hpy188I TCNGA 9 cut(s) 66, 177, 361, 495, 660, 1117, 1347, 1525, 1564
Hpy8I GTNNAC 4 cut(s) 253, 314, 795, 1609
Hpy99I CGWCG 1 cut(s) 432
HpyAV CCTTC 6 cut(s) 25, 148, 634, 781, 1012, 1054
HpyCH4III ACNGT 5 cut(s) 742, 1355, 1579, 1613, 1648
HpyCH4IV ACGT 1 cut(s) 430
HpyF10VI GCNNNNNNNGC 4 cut(s) 1022, 1070, 1247, 1673
HpyF3I CTNAG 8 cut(s) 45, 156, 494, 657, 849, 1106, 1448, 1486
HpySE526I ACGT 1 cut(s) 430
HspAI GCGC 1 cut(s) 1515
Ksp22I TGATCA 1 cut(s) 271
Kzo9I GATC 9 cut(s) 94, 239, 271, 367, 667, 709, 934, 1435, 1702
LmnI GCTCC 2 cut(s) 29, 424
Lsp1109I GCAGC 5 cut(s) 116, 198, 245, 539, 1325
LweI GCATC 5 cut(s) 835, 1012, 1143, 1491, 1529
MaeI CTAG 5 cut(s) 183, 1223, 1467, 1535, 1634
MaeII ACGT 1 cut(s) 430
MaeIII GTNAC 3 cut(s) 940, 1131, 1566
MalI GATC 9 cut(s) 96, 241, 273, 369, 669, 711, 936, 1437, 1704
MboI GATC 9 cut(s) 94, 239, 271, 367, 667, 709, 934, 1435, 1702
MflI RGATCY 3 cut(s) 239, 709, 1702
MhlI GDGCHC 1 cut(s) 630
MlsI TGGCCA 2 cut(s) 381, 1359
MluCI AATT 7 cut(s) 85, 281, 331, 395, 1065, 1324, 1395
MluNI TGGCCA 2 cut(s) 381, 1359
MlyI GAGTC 8 cut(s) 169, 172, 230, 770, 800, 893, 1301, 1574
MmeI TCCRAC 1 cut(s) 660
Mox20I TGGCCA 2 cut(s) 381, 1359
Mph1103I ATGCAT 1 cut(s) 1038
MroXI GAANNNNTTC 1 cut(s) 489
MscI TGGCCA 2 cut(s) 381, 1359
MseI TTAA 3 cut(s) 9, 444, 981
MslI CAYNNNNRTG 2 cut(s) 899, 1041
Msp20I TGGCCA 2 cut(s) 381, 1359
MspA1I CMGCKG 4 cut(s) 107, 530, 706, 1316
MvnI CGCG 1 cut(s) 1517
MwoI GCNNNNNNNGC 4 cut(s) 1022, 1070, 1247, 1673
NcoI CCATGG 1 cut(s) 879
NdeI CATATG 1 cut(s) 809
NdeII GATC 9 cut(s) 94, 239, 271, 367, 667, 709, 934, 1435, 1702
NlaIV GGNNCC 2 cut(s) 295, 711
NmeAIII GCCGAG 2 cut(s) 484, 1234
NmuCI GTSAC 3 cut(s) 940, 1131, 1566
NsiI ATGCAT 1 cut(s) 1038
NspI RCATGY 4 cut(s) 731, 1016, 1036, 1040
PaeI GCATGC 1 cut(s) 1040
PagI TCATGA 1 cut(s) 607
PdmI GAANNNNTTC 1 cut(s) 489
PfeI GAWTC 2 cut(s) 343, 1235
PkrI GCNGC 7 cut(s) 106, 188, 235, 529, 955, 1315, 1519
PleI GAGTC 8 cut(s) 168, 172, 229, 769, 799, 892, 1300, 1573
PpsI GAGTC 8 cut(s) 168, 172, 229, 769, 799, 892, 1300, 1573
PshBI ATTAAT 1 cut(s) 444
PspFI CCCAGC 1 cut(s) 545
PspN4I GGNNCC 2 cut(s) 295, 711
PspPI GGNCC 1 cut(s) 1351
PstI CTGCAG 1 cut(s) 202
PstNI CAGNNNCTG 2 cut(s) 530, 740
PsuI RGATCY 3 cut(s) 239, 709, 1702
PvuII CAGCTG 3 cut(s) 107, 530, 706
RsaI GTAC 2 cut(s) 313, 588
RsaNI GTAC 2 cut(s) 312, 587
RseI CAYNNNNRTG 2 cut(s) 899, 1041
SaqAI TTAA 3 cut(s) 9, 444, 981
SatI GCNGC 7 cut(s) 105, 187, 234, 528, 954, 1314, 1518
Sau3AI GATC 9 cut(s) 94, 239, 271, 367, 667, 709, 934, 1435, 1702
Sau96I GGNCC 1 cut(s) 1351
SchI GAGTC 8 cut(s) 169, 172, 230, 770, 800, 893, 1301, 1574
SduI GDGCHC 1 cut(s) 630
SfaNI GCATC 5 cut(s) 835, 1012, 1143, 1491, 1529
SfcI CTRYAG 2 cut(s) 78, 198
SinI GGWCC 1 cut(s) 1351
SmiMI CAYNNNNRTG 2 cut(s) 899, 1041
SphI GCATGC 1 cut(s) 1040
Sse9I AATT 7 cut(s) 85, 281, 331, 395, 1065, 1324, 1395
SsiI CCGC 5 cut(s) 14, 954, 1316, 1336, 1517
SspI AATATT 1 cut(s) 817
SspMI CTAG 5 cut(s) 183, 1223, 1467, 1535, 1634
StyI CCWWGG 2 cut(s) 750, 879
TaaI ACNGT 5 cut(s) 742, 1355, 1579, 1613, 1648
TaiI ACGT 1 cut(s) 433
TaqI TCGA 4 cut(s) 540, 828, 1099, 1656
TasI AATT 7 cut(s) 85, 281, 331, 395, 1065, 1324, 1395
TatI WGTACW 2 cut(s) 311, 586
TauI GCSGC 2 cut(s) 956, 1520
TfiI GAWTC 2 cut(s) 343, 1235
Tru1I TTAA 3 cut(s) 9, 444, 981
Tru9I TTAA 3 cut(s) 9, 444, 981
TscAI CASTG 4 cut(s) 250, 321, 412, 1308
TseFI GTSAC 3 cut(s) 940, 1131, 1566
TseI GCWGC 5 cut(s) 104, 186, 233, 527, 1313
Tsp45I GTSAC 3 cut(s) 940, 1131, 1566
TspGWI ACGGA 1 cut(s) 1258
TspRI CASTG 4 cut(s) 250, 321, 412, 1308
VpaK11BI GGWCC 1 cut(s) 1351
VspI ATTAAT 1 cut(s) 444
XagI CCTNNNNNAGG 1 cut(s) 749
XapI RAATTY 5 cut(s) 85, 281, 331, 395, 1324
XbaI TCTAGA 1 cut(s) 1534
XceI RCATGY 4 cut(s) 731, 1016, 1036, 1040
XmnI GAANNNNTTC 1 cut(s) 489
XspI CTAG 5 cut(s) 183, 1223, 1467, 1535, 1634
Zsp2I ATGCAT 1 cut(s) 1038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.