Rmu_sc0003306.1_g000008

Phospholipid diacylglycerol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003306.1
OG0011775
Physical Location & Seq
Forward (+)
41787 .. 43601
1815 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1053 bp
atggctgcttcaattcttcggcttcgcaagctgtgctatgttcagcctgtgaggttttcgacattgtgctttcagtcctttgaaacagccaaggacgagggtgctgaaactccgaagttcgaaaatgaagtgaaagagaaacagaggattaagaacaagaacaagaggcaaccgagggaatggagatgcatacataactgttgttggataattgggtccatgtgtacaacttggtggcttcttttgtttttgtacaacaccttgccaactgcattgactggttttcaggtgccagaatcacctggaacaaggcttaggcgcgaaggtgtgccagaatcacctggaacaaggcttaggcgcgaaggtgtaagcctccttcacccagttgtcatggtccctggcattgtaaccggtggcctagagctctgggagggcaggccttgtgctgaaggtctttttaggaagcgactttggggtggtagtttctcagagatcttcaaaaggcctatgtgtttgttggagcacctatcactggacaatgaaacagggctggaccctccaggaattcgagtcagagcagttcggggactcgctgcagctgactattttgctcctggctactttgtttgggctgtcctcattaagaatttggcaaaaattggttatgaagggaagaacatgcatatggctgcctatgattggaggctttctttccagaacactgagattcgggaccaagctcttagtaggttgaagagtaaaattgagcttatgtatgcaatcaatggccataagaaagtggtcgtggtgcctcattctatgggggctatttattttctccacttcatgaaatgggtcgagtcacctcctcctatgggaggtggtggtggtccaagttggtgtgccacgcacatcaaagcaatcatgaatatcggtccagcatttcttggtgttccaagagcagttagtaatatattttctgctgagggtaaagatgttgcttacatcaggtttgtctatgtgttcaatttctcatgtgtgtttgcattctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 map00561
Pfam Domains
Protein Families
LCAT Lipase_GDSL

Protein Analysis

350

Amino Acids

39.61

Weight (kDa)

9.51

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 153 - 335 1.4e-39 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011775)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44830
fragaria_vesca FvH4_3g30050
malus_domestica MD11G1154200.v1.1
prunus_persica Prupe.6G116700_v2.0.a1 Prupe.6G116700_v2.0.a1
pyrus_communis pycom11g12590
rosa_chinensis RchiOBHm_Chr5g0055851 RchiOBHm_Chr5g0055861
rosa_laevigata RLG00000035014
rosa_multiflora Rmu_sc0003306.1_g000008
rosa_roxburghii Rroxscaffold_1G00024510
rosa_rugosa Rorug05G0295200 Rorug05G0295200 Rorug05G0295300
rosa_samantha Rh5AG366600 Rh5BG376800 Rh5CG399800 Rh5DG389800
rosa_wichuraiana Rw5G034360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 289, 808
AccII CGCG 2 cut(s) 321, 360
AcoI YGGCCR 1 cut(s) 787
AcsI RAATTY 2 cut(s) 564, 646
AcuI CTGAAG 1 cut(s) 468
AfaI GTAC 2 cut(s) 226, 254
AfiI CCNNNNNNNGG 4 cut(s) 532, 699, 875, 878
AgeI ACCGGT 1 cut(s) 410
AgsI TTSAA 5 cut(s) 12, 83, 499, 754, 1027
AjnI CCWGG 5 cut(s) 301, 340, 397, 559, 613
AluBI AGCT 5 cut(s) 31, 424, 599, 740, 769
AluI AGCT 5 cut(s) 31, 424, 599, 740, 769
Alw21I GWGCWC 2 cut(s) 426, 525
AoxI GGCC 4 cut(s) 415, 437, 503, 787
ApeKI GCWGC 4 cut(s) 5, 593, 596, 689
ApoI RAATTY 2 cut(s) 564, 646
AsiGI ACCGGT 1 cut(s) 410
AspLEI GCGC 2 cut(s) 321, 360
AspS9I GGNCC 6 cut(s) 216, 394, 553, 733, 890, 935
AsuHPI GGTGA 4 cut(s) 291, 330, 371, 855
AsuII TTCGAA 1 cut(s) 120
AvaII GGWCC 6 cut(s) 216, 394, 553, 733, 890, 935
BalI TGGCCA 1 cut(s) 789
BanI GGYRCC 2 cut(s) 289, 808
BanII GRGCYC 1 cut(s) 426
BarI GAAGNNNNNNTAC 2 cut(s) 360, 392
Bbv12I GWGCWC 2 cut(s) 426, 525
BbvCI CCTCAGC 1 cut(s) 984
BbvI GCAGC 3 cut(s) 580, 608, 676
BciT130I CCWGG 5 cut(s) 303, 342, 399, 561, 615
BfaI CTAG 1 cut(s) 419
BfmI CTRYAG 1 cut(s) 594
BglII AGATCT 1 cut(s) 492
BisI GCNGC 4 cut(s) 6, 594, 597, 690
BlsI GCNGC 4 cut(s) 7, 595, 598, 691
Bme1390I CCNGG 5 cut(s) 303, 342, 399, 561, 615
Bme18I GGWCC 6 cut(s) 216, 394, 553, 733, 890, 935
BmgT120I GGNCC 6 cut(s) 216, 394, 553, 733, 890, 935
BmiI GGNNCC 6 cut(s) 217, 291, 396, 555, 734, 810
BmrFI CCNGG 5 cut(s) 303, 342, 399, 561, 615
BmrI ACTGGG 1 cut(s) 377
BmsI GCATC 1 cut(s) 176
BmuI ACTGGG 1 cut(s) 377
BpmI CTGGAG 1 cut(s) 543
Bpu10I CCTNAGC 3 cut(s) 314, 353, 984
Bpu14I TTCGAA 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 90, 173, 397
BsaWI WCCGGW 1 cut(s) 410
Bsc4I CCNNNNNNNGG 4 cut(s) 532, 699, 875, 878
Bse118I RCCGGY 1 cut(s) 410
Bse1I ACTGG 3 cut(s) 283, 383, 537
BseBI CCWGG 5 cut(s) 303, 342, 399, 561, 615
BseDI CCNNGG 3 cut(s) 90, 173, 397
BseLI CCNNNNNNNGG 4 cut(s) 532, 699, 875, 878
BseMII CTCAG 3 cut(s) 501, 714, 975
BseNI ACTGG 3 cut(s) 283, 383, 537
BseRI GAGGAG 1 cut(s) 858
BseXI GCAGC 3 cut(s) 580, 608, 676
Bsh1236I CGCG 2 cut(s) 321, 360
BshFI GGCC 4 cut(s) 417, 439, 505, 789
BshNI GGYRCC 2 cut(s) 289, 808
BshTI ACCGGT 1 cut(s) 410
BsiHKAI GWGCWC 2 cut(s) 426, 525
BsiSI CCGG 1 cut(s) 411
BslFI GGGAC 3 cut(s) 380, 600, 746
BslI CCNNNNNNNGG 4 cut(s) 532, 699, 875, 878
BsmFI GGGAC 3 cut(s) 380, 600, 746
BsmI GAATGC 1 cut(s) 1046
BsnI GGCC 4 cut(s) 417, 439, 505, 789
Bsp119I TTCGAA 1 cut(s) 120
Bsp1286I GDGCHC 2 cut(s) 426, 525
Bsp1407I TGTACA 2 cut(s) 224, 252
Bsp143I GATC 1 cut(s) 492
BspANI GGCC 4 cut(s) 417, 439, 505, 789
BspCNI CTCAG 3 cut(s) 500, 715, 976
BspFNI CGCG 2 cut(s) 321, 360
BspHI TCATGA 2 cut(s) 846, 924
BspLI GGNNCC 6 cut(s) 217, 291, 396, 555, 734, 810
BspMAI CTGCAG 1 cut(s) 598
BspT104I TTCGAA 1 cut(s) 120
BspT107I GGYRCC 2 cut(s) 289, 808
BsrFI RCCGGY 1 cut(s) 410
BsrGI TGTACA 2 cut(s) 224, 252
BsrI ACTGG 3 cut(s) 283, 383, 537
BssAI RCCGGY 1 cut(s) 410
BssECI CCNNGG 3 cut(s) 90, 173, 397
BssMI GATC 1 cut(s) 492
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 5 cut(s) 303, 342, 399, 561, 615
Bst4CI ACNGT 1 cut(s) 200
Bst6I CTCTTC 1 cut(s) 749
BstAPI GCANNNNNTGC 1 cut(s) 33
BstAUI TGTACA 2 cut(s) 224, 252
BstBI TTCGAA 1 cut(s) 120
BstC8I GCNNGC 2 cut(s) 29, 437
BstDEI CTNAG 6 cut(s) 314, 353, 487, 723, 743, 984
BstENI CCTNNNNNAGG 1 cut(s) 876
BstFNI CGCG 2 cut(s) 321, 360
BstHHI GCGC 2 cut(s) 321, 360
BstKTI GATC 1 cut(s) 495
BstMBI GATC 1 cut(s) 492
BstMWI GCNNNNNNNGC 2 cut(s) 28, 33
BstNI CCWGG 5 cut(s) 303, 342, 399, 561, 615
BstNSI RCATGY 1 cut(s) 682
BstSCI CCNGG 5 cut(s) 301, 340, 397, 559, 613
BstSFI CTRYAG 1 cut(s) 594
BstUI CGCG 2 cut(s) 321, 360
BstV1I GCAGC 3 cut(s) 580, 608, 676
BstX2I RGATCY 1 cut(s) 492
BstYI RGATCY 1 cut(s) 492
BsuRI GGCC 4 cut(s) 417, 439, 505, 789
BtsIMutI CAGTG 2 cut(s) 530, 720
Cac8I GCNNGC 2 cut(s) 29, 437
CciI TCATGA 2 cut(s) 846, 924
CfoI GCGC 2 cut(s) 321, 360
Cfr10I RCCGGY 1 cut(s) 410
Cfr13I GGNCC 6 cut(s) 216, 394, 553, 733, 890, 935
Csp6I GTAC 2 cut(s) 225, 253
CspAI ACCGGT 1 cut(s) 410
CviAII CATG 6 cut(s) 220, 391, 679, 847, 925, 1035
CviQI GTAC 2 cut(s) 225, 253
DdeI CTNAG 6 cut(s) 314, 353, 487, 723, 743, 984
DpnI GATC 1 cut(s) 494
DpnII GATC 1 cut(s) 492
EaeI YGGCCR 1 cut(s) 787
Eam1104I CTCTTC 1 cut(s) 749
EarI CTCTTC 1 cut(s) 749
Ecl136II GAGCTC 1 cut(s) 424
Eco130I CCWWGG 1 cut(s) 90
Eco147I AGGCCT 2 cut(s) 439, 505
Eco24I GRGCYC 1 cut(s) 426
Eco47I GGWCC 6 cut(s) 216, 394, 553, 733, 890, 935
Eco53kI GAGCTC 1 cut(s) 424
Eco57I CTGAAG 1 cut(s) 468
EcoICRI GAGCTC 1 cut(s) 424
EcoNI CCTNNNNNAGG 1 cut(s) 876
EcoRI GAATTC 1 cut(s) 564
EcoRII CCWGG 5 cut(s) 301, 340, 397, 559, 613
EcoT14I CCWWGG 1 cut(s) 90
EcoT22I ATGCAT 2 cut(s) 191, 684
EcoT38I GRGCYC 1 cut(s) 426
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 6 cut(s) 223, 394, 682, 850, 928, 1038
FaqI GGGAC 3 cut(s) 380, 600, 746
FatI CATG 6 cut(s) 219, 390, 678, 846, 924, 1034
FauNDI CATATG 1 cut(s) 684
Fnu4HI GCNGC 4 cut(s) 6, 594, 597, 690
FriOI GRGCYC 1 cut(s) 426
Fsp4HI GCNGC 4 cut(s) 6, 594, 597, 690
FspBI CTAG 1 cut(s) 419
GlaI GCGC 2 cut(s) 320, 359
GluI GCNGC 4 cut(s) 6, 594, 597, 690
GsuI CTGGAG 1 cut(s) 543
HaeIII GGCC 4 cut(s) 417, 439, 505, 789
HapII CCGG 1 cut(s) 411
HhaI GCGC 2 cut(s) 321, 360
Hin1II CATG 6 cut(s) 223, 394, 682, 850, 928, 1038
Hin6I GCGC 2 cut(s) 319, 358
HinP1I GCGC 2 cut(s) 319, 358
HinfI GANTC 6 cut(s) 296, 335, 570, 588, 727, 860
HpaII CCGG 1 cut(s) 411
HphI GGTGA 4 cut(s) 291, 330, 371, 855
Hpy166II GTNNAC 1 cut(s) 225
Hpy188I TCNGA 4 cut(s) 114, 490, 575, 1052
Hpy188III TCNNGA 4 cut(s) 715, 731, 847, 925
Hpy8I GTNNAC 1 cut(s) 225
HpyAV CCTTC 5 cut(s) 317, 356, 386, 443, 662
HpyCH4III ACNGT 1 cut(s) 200
HpyCH4V TGCA 6 cut(s) 189, 272, 596, 682, 779, 1046
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 33
HpyF3I CTNAG 6 cut(s) 314, 353, 487, 723, 743, 984
Hsp92II CATG 6 cut(s) 223, 394, 682, 850, 928, 1038
HspAI GCGC 2 cut(s) 319, 358
Kzo9I GATC 1 cut(s) 492
LmnI GCTCC 2 cut(s) 520, 616
Lsp1109I GCAGC 3 cut(s) 580, 608, 676
LweI GCATC 1 cut(s) 176
MaeI CTAG 1 cut(s) 419
MaeIII GTNAC 2 cut(s) 406, 861
MalI GATC 1 cut(s) 494
MboI GATC 1 cut(s) 492
MboII GAAGA 4 cut(s) 8, 487, 685, 766
MflI RGATCY 1 cut(s) 492
MhlI GDGCHC 2 cut(s) 426, 525
MlsI TGGCCA 1 cut(s) 789
MluCI AATT 7 cut(s) 12, 210, 564, 646, 657, 762, 1027
MluNI TGGCCA 1 cut(s) 789
MlyI GAGTC 3 cut(s) 579, 582, 869
MmeI TCCRAC 2 cut(s) 185, 498
Mox20I TGGCCA 1 cut(s) 789
Mph1103I ATGCAT 2 cut(s) 191, 684
MscI TGGCCA 1 cut(s) 789
MseI TTAA 2 cut(s) 150, 642
MslI CAYNNNNRTG 1 cut(s) 683
Msp20I TGGCCA 1 cut(s) 789
MspA1I CMGCKG 1 cut(s) 599
MspI CCGG 1 cut(s) 411
MspR9I CCNGG 5 cut(s) 303, 342, 399, 561, 615
Mva1269I GAATGC 1 cut(s) 1046
MvaI CCWGG 5 cut(s) 303, 342, 399, 561, 615
MvnI CGCG 2 cut(s) 321, 360
MwoI GCNNNNNNNGC 2 cut(s) 28, 33
NdeI CATATG 1 cut(s) 684
NdeII GATC 1 cut(s) 492
NlaIII CATG 6 cut(s) 223, 394, 682, 850, 928, 1038
NlaIV GGNNCC 6 cut(s) 217, 291, 396, 555, 734, 810
NmuCI GTSAC 1 cut(s) 861
NsiI ATGCAT 2 cut(s) 191, 684
NspI RCATGY 1 cut(s) 682
NspV TTCGAA 1 cut(s) 120
PagI TCATGA 2 cut(s) 846, 924
PceI AGGCCT 2 cut(s) 439, 505
PctI GAATGC 1 cut(s) 1046
PfeI GAWTC 3 cut(s) 296, 335, 727
PfoI TCCNGGA 1 cut(s) 559
PinAI ACCGGT 1 cut(s) 410
PkrI GCNGC 4 cut(s) 7, 595, 598, 691
PleI GAGTC 3 cut(s) 578, 582, 868
PpsI GAGTC 3 cut(s) 578, 582, 868
Psp124BI GAGCTC 1 cut(s) 426
Psp6I CCWGG 5 cut(s) 301, 340, 397, 559, 613
PspGI CCWGG 5 cut(s) 301, 340, 397, 559, 613
PspN4I GGNNCC 6 cut(s) 217, 291, 396, 555, 734, 810
PspPI GGNCC 6 cut(s) 216, 394, 553, 733, 890, 935
PstI CTGCAG 1 cut(s) 598
PsuI RGATCY 1 cut(s) 492
PvuII CAGCTG 1 cut(s) 599
RsaI GTAC 2 cut(s) 226, 254
RsaNI GTAC 2 cut(s) 225, 253
RseI CAYNNNNRTG 1 cut(s) 683
SacI GAGCTC 1 cut(s) 426
SaqAI TTAA 2 cut(s) 150, 642
SatI GCNGC 4 cut(s) 6, 594, 597, 690
Sau3AI GATC 1 cut(s) 492
Sau96I GGNCC 6 cut(s) 216, 394, 553, 733, 890, 935
SchI GAGTC 3 cut(s) 579, 582, 869
ScrFI CCNGG 5 cut(s) 303, 342, 399, 561, 615
SduI GDGCHC 2 cut(s) 426, 525
SfaNI GCATC 1 cut(s) 176
SfcI CTRYAG 1 cut(s) 594
SfuI TTCGAA 1 cut(s) 120
SinI GGWCC 6 cut(s) 216, 394, 553, 733, 890, 935
SmiMI CAYNNNNRTG 1 cut(s) 683
Sse9I AATT 7 cut(s) 12, 210, 564, 646, 657, 762, 1027
SseBI AGGCCT 2 cut(s) 439, 505
SspMI CTAG 1 cut(s) 419
SstI GAGCTC 1 cut(s) 426
StuI AGGCCT 2 cut(s) 439, 505
StyD4I CCNGG 5 cut(s) 301, 340, 397, 559, 613
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 1 cut(s) 200
TaqI TCGA 4 cut(s) 59, 120, 568, 858
TaqII GACCGA 1 cut(s) 923
TasI AATT 7 cut(s) 12, 210, 564, 646, 657, 762, 1027
TatI WGTACW 2 cut(s) 224, 252
TfiI GAWTC 3 cut(s) 296, 335, 727
Tru1I TTAA 2 cut(s) 150, 642
Tru9I TTAA 2 cut(s) 150, 642
TscAI CASTG 2 cut(s) 537, 727
TseFI GTSAC 1 cut(s) 861
TseI GCWGC 4 cut(s) 5, 593, 596, 689
Tsp45I GTSAC 1 cut(s) 861
TspDTI ATGAA 6 cut(s) 141, 555, 681, 835, 863, 941
TspRI CASTG 2 cut(s) 537, 727
VpaK11BI GGWCC 6 cut(s) 216, 394, 553, 733, 890, 935
XagI CCTNNNNNAGG 1 cut(s) 876
XapI RAATTY 2 cut(s) 564, 646
XceI RCATGY 1 cut(s) 682
XspI CTAG 1 cut(s) 419
Zsp2I ATGCAT 2 cut(s) 191, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.