Rmu_sc0003687.1_g000008

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003687.1
OG0008907
Physical Location & Seq
Forward (+)
25603 .. 26491
889 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 600 bp
atgtccgacgaagaaatcctgaacttcatcaatggaaagtattctccggggtacagcactgcctttgatgttggtgatcttttcacgatcactaagaccatcattgatcatgcaacccgtaattcgatggtcgatgatattctgaagtcggccaatgccctgaatgagaaggtccccaacatcaacgacagcttcatctcgccattgtgcactcttaagtccatttgctgcgagatgtcatcatgtatggataaggcttgttctggtcaagaaatttccatccaaccaaggacggaagtgatatttgacaagctaaaaccctatccatgggacgctaaggcagtgctggccctagcggcttttgctttggaatacggagacttctggcacctcgcccagctctatggccaatgtaaccaactcaccaattcactggctatcctgaccaagcatgcgaccttggagaaacgcaaggaggaagtacttgagctgaaccatctcatcaaggctactttgcaagtgactgatttcatctttaaggttgaggatctttttgttcacaatcataacgagaatgtaaaagagttgacaaggcagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

199

Amino Acids

22.49

Weight (kDa)

5.22

Isoelectric Point (pI)

40.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 2 - 197 6e-34 Sieve element occlusion N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 387
AciI CCGC 1 cut(s) 356
AclWI GGATC 1 cut(s) 555
AcoI YGGCCR 2 cut(s) 150, 406
AcsI RAATTY 1 cut(s) 273
AcuI CTGAAG 1 cut(s) 164
AfaI GTAC 2 cut(s) 53, 483
AfiI CCNNNNNNNGG 1 cut(s) 327
AflII CTTAAG 1 cut(s) 215
AluBI AGCT 4 cut(s) 192, 313, 400, 490
AluI AGCT 4 cut(s) 192, 313, 400, 490
Alw21I GWGCWC 1 cut(s) 212
Alw26I GTCTC 1 cut(s) 372
Alw44I GTGCAC 1 cut(s) 208
AlwI GGATC 1 cut(s) 555
AoxI GGCC 3 cut(s) 150, 348, 406
ApaLI GTGCAC 1 cut(s) 208
ApeKI GCWGC 1 cut(s) 228
ApoI RAATTY 1 cut(s) 273
Asp700I GAANNNNTTC 1 cut(s) 40
AspS9I GGNCC 2 cut(s) 172, 349
AsuC2I CCSGG 1 cut(s) 48
AsuHPI GGTGA 2 cut(s) 86, 415
AvaII GGWCC 1 cut(s) 172
BaeGI GKGCMC 1 cut(s) 212
BalI TGGCCA 1 cut(s) 408
BanI GGYRCC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 212
BbvI GCAGC 1 cut(s) 215
BccI CCATC 4 cut(s) 107, 121, 287, 504
BclI TGATCA 1 cut(s) 106
BcnI CCSGG 1 cut(s) 48
BcoDI GTCTC 1 cut(s) 372
BfaI CTAG 1 cut(s) 353
BfrI CTTAAG 1 cut(s) 215
BglI GCCNNNNNGGC 1 cut(s) 356
BisI GCNGC 2 cut(s) 229, 357
BlsI GCNGC 2 cut(s) 230, 358
BmcAI AGTACT 1 cut(s) 483
Bme1390I CCNGG 1 cut(s) 48
Bme18I GGWCC 1 cut(s) 172
BmgT120I GGNCC 2 cut(s) 172, 349
BmiI GGNNCC 2 cut(s) 174, 389
BmrFI CCNGG 1 cut(s) 48
Bpu10I CCTNAGC 1 cut(s) 336
BpuEI CTTGAG 1 cut(s) 506
BpuMI CCSGG 1 cut(s) 48
BsaJI CCNNGG 4 cut(s) 47, 287, 326, 459
Bsc4I CCNNNNNNNGG 1 cut(s) 327
Bse1I ACTGG 1 cut(s) 438
BseDI CCNNGG 4 cut(s) 47, 287, 326, 459
BseGI GGATG 1 cut(s) 279
BseLI CCNNNNNNNGG 1 cut(s) 327
BseNI ACTGG 1 cut(s) 438
BseSI GKGCMC 1 cut(s) 212
BseXI GCAGC 1 cut(s) 215
BseYI CCCAGC 1 cut(s) 396
BshFI GGCC 3 cut(s) 152, 350, 408
BshNI GGYRCC 1 cut(s) 387
BsiHKAI GWGCWC 1 cut(s) 212
BsiSI CCGG 1 cut(s) 47
BslFI GGGAC 2 cut(s) 158, 344
BslI CCNNNNNNNGG 1 cut(s) 327
BsmAI GTCTC 1 cut(s) 372
BsmFI GGGAC 2 cut(s) 158, 344
BsnI GGCC 3 cut(s) 152, 350, 408
Bsp1286I GDGCHC 1 cut(s) 212
Bsp143I GATC 4 cut(s) 76, 87, 106, 547
Bsp19I CCATGG 1 cut(s) 326
BspACI CCGC 1 cut(s) 356
BspANI GGCC 3 cut(s) 152, 350, 408
BspLI GGNNCC 2 cut(s) 174, 389
BspPI GGATC 1 cut(s) 555
BspT107I GGYRCC 1 cut(s) 387
BspTI CTTAAG 1 cut(s) 215
BsrI ACTGG 1 cut(s) 438
BssECI CCNNGG 4 cut(s) 47, 287, 326, 459
BssMI GATC 4 cut(s) 76, 87, 106, 547
BssT1I CCWWGG 3 cut(s) 287, 326, 459
BstAFI CTTAAG 1 cut(s) 215
BstC8I GCNNGC 2 cut(s) 348, 453
BstDEI CTNAG 2 cut(s) 93, 336
BstDSI CCRYGG 1 cut(s) 326
BstF5I GGATG 1 cut(s) 279
BstKTI GATC 4 cut(s) 79, 90, 109, 550
BstMAI GTCTC 1 cut(s) 372
BstMBI GATC 4 cut(s) 76, 87, 106, 547
BstMWI GCNNNNNNNGC 3 cut(s) 347, 356, 362
BstNSI RCATGY 1 cut(s) 455
BstSCI CCNGG 1 cut(s) 46
BstSLI GKGCMC 1 cut(s) 212
BstV1I GCAGC 1 cut(s) 215
BstX2I RGATCY 1 cut(s) 547
BstXI CCANNNNNNTGG 2 cut(s) 404, 433
BstYI RGATCY 1 cut(s) 547
BsuRI GGCC 3 cut(s) 152, 350, 408
BtgI CCRYGG 1 cut(s) 326
BtsCI GGATG 1 cut(s) 279
BtsI GCAGTG 2 cut(s) 57, 348
BtsIMutI CAGTG 3 cut(s) 57, 348, 431
Cac8I GCNNGC 2 cut(s) 348, 453
Cfr13I GGNCC 2 cut(s) 172, 349
CseI GACGC 1 cut(s) 341
Csp6I GTAC 2 cut(s) 52, 482
CviAII CATG 4 cut(s) 110, 243, 327, 452
CviQI GTAC 2 cut(s) 52, 482
DdeI CTNAG 2 cut(s) 93, 336
DpnI GATC 4 cut(s) 78, 89, 108, 549
DpnII GATC 4 cut(s) 76, 87, 106, 547
EaeI YGGCCR 2 cut(s) 150, 406
Eco130I CCWWGG 3 cut(s) 287, 326, 459
Eco47I GGWCC 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 164
EcoO109I RGGNCCY 1 cut(s) 172
EcoT14I CCWWGG 3 cut(s) 287, 326, 459
ErhI CCWWGG 3 cut(s) 287, 326, 459
FaeI CATG 4 cut(s) 113, 246, 330, 455
FaiI YATR 7 cut(s) 111, 244, 248, 328, 405, 453, 567
FaqI GGGAC 2 cut(s) 158, 344
FatI CATG 4 cut(s) 109, 242, 326, 451
FbaI TGATCA 1 cut(s) 106
Fnu4HI GCNGC 2 cut(s) 229, 357
FokI GGATG 1 cut(s) 266
Fsp4HI GCNGC 2 cut(s) 229, 357
FspBI CTAG 1 cut(s) 353
GluI GCNGC 2 cut(s) 229, 357
GsaI CCCAGC 1 cut(s) 400
HaeIII GGCC 3 cut(s) 152, 350, 408
HapII CCGG 1 cut(s) 47
HgaI GACGC 1 cut(s) 341
Hin1II CATG 4 cut(s) 113, 246, 330, 455
HincII GTYRAC 1 cut(s) 588
HindII GTYRAC 1 cut(s) 588
HpaII CCGG 1 cut(s) 47
HphI GGTGA 2 cut(s) 86, 415
Hpy166II GTNNAC 3 cut(s) 210, 559, 588
Hpy188I TCNGA 2 cut(s) 7, 144
Hpy188III TCNNGA 4 cut(s) 19, 85, 269, 442
Hpy8I GTNNAC 3 cut(s) 210, 559, 588
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 1 cut(s) 163
HpyCH4V TGCA 3 cut(s) 113, 210, 517
HpyF10VI GCNNNNNNNGC 3 cut(s) 347, 356, 362
HpyF3I CTNAG 2 cut(s) 93, 336
Hsp92II CATG 4 cut(s) 113, 246, 330, 455
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 4 cut(s) 76, 87, 106, 547
LpnPI CCDG 9 cut(s) 32, 60, 173, 249, 332, 370, 410, 419, 455
Lsp1109I GCAGC 1 cut(s) 215
MaeI CTAG 1 cut(s) 353
MaeIII GTNAC 2 cut(s) 413, 520
MalI GATC 4 cut(s) 78, 89, 108, 549
MboI GATC 4 cut(s) 76, 87, 106, 547
MboII GAAGA 1 cut(s) 23
MflI RGATCY 1 cut(s) 547
MhlI GDGCHC 1 cut(s) 212
MlsI TGGCCA 1 cut(s) 408
MluCI AATT 3 cut(s) 121, 273, 427
MluNI TGGCCA 1 cut(s) 408
MmeI TCCRAC 2 cut(s) 30, 307
MnlI CCTC 3 cut(s) 401, 469, 538
Mox20I TGGCCA 1 cut(s) 408
MroXI GAANNNNTTC 1 cut(s) 40
MscI TGGCCA 1 cut(s) 408
MseI TTAA 2 cut(s) 216, 537
Msp20I TGGCCA 1 cut(s) 408
MspCI CTTAAG 1 cut(s) 215
MspI CCGG 1 cut(s) 47
MspR9I CCNGG 1 cut(s) 48
MwoI GCNNNNNNNGC 3 cut(s) 347, 356, 362
NciI CCSGG 1 cut(s) 48
NcoI CCATGG 1 cut(s) 326
NdeII GATC 4 cut(s) 76, 87, 106, 547
NlaIII CATG 4 cut(s) 113, 246, 330, 455
NlaIV GGNNCC 2 cut(s) 174, 389
NmuCI GTSAC 1 cut(s) 520
NspI RCATGY 1 cut(s) 455
PaeI GCATGC 1 cut(s) 455
PdmI GAANNNNTTC 1 cut(s) 40
PkrI GCNGC 2 cut(s) 230, 358
PpuMI RGGWCCY 1 cut(s) 172
Psp5II RGGWCCY 1 cut(s) 172
PspFI CCCAGC 1 cut(s) 396
PspN4I GGNNCC 2 cut(s) 174, 389
PspPI GGNCC 2 cut(s) 172, 349
PspPPI RGGWCCY 1 cut(s) 172
PsuI RGATCY 1 cut(s) 547
RsaI GTAC 2 cut(s) 53, 483
RsaNI GTAC 2 cut(s) 52, 482
SaqAI TTAA 2 cut(s) 216, 537
SatI GCNGC 2 cut(s) 229, 357
Sau3AI GATC 4 cut(s) 76, 87, 106, 547
Sau96I GGNCC 2 cut(s) 172, 349
ScaI AGTACT 1 cut(s) 483
ScrFI CCNGG 1 cut(s) 48
SduI GDGCHC 1 cut(s) 212
SetI ASST 8 cut(s) 174, 194, 315, 393, 402, 461, 492, 543
SinI GGWCC 1 cut(s) 172
SmlI CTYRAG 2 cut(s) 215, 485
SmoI CTYRAG 2 cut(s) 215, 485
SphI GCATGC 1 cut(s) 455
Sse9I AATT 3 cut(s) 121, 273, 427
SsiI CCGC 1 cut(s) 356
SspMI CTAG 1 cut(s) 353
StyD4I CCNGG 1 cut(s) 46
StyI CCWWGG 3 cut(s) 287, 326, 459
TaqI TCGA 2 cut(s) 125, 132
TasI AATT 3 cut(s) 121, 273, 427
TatI WGTACW 1 cut(s) 481
TauI GCSGC 1 cut(s) 359
Tru1I TTAA 2 cut(s) 216, 537
Tru9I TTAA 2 cut(s) 216, 537
TscAI CASTG 3 cut(s) 64, 348, 438
TseFI GTSAC 1 cut(s) 520
TseI GCWGC 1 cut(s) 228
Tsp45I GTSAC 1 cut(s) 520
TspDTI ATGAA 3 cut(s) 16, 184, 520
TspGWI ACGGA 2 cut(s) 308, 390
TspRI CASTG 3 cut(s) 64, 348, 438
Vha464I CTTAAG 1 cut(s) 215
VneI GTGCAC 1 cut(s) 208
VpaK11BI GGWCC 1 cut(s) 172
XapI RAATTY 1 cut(s) 273
XceI RCATGY 1 cut(s) 455
XmnI GAANNNNTTC 1 cut(s) 40
XspI CTAG 1 cut(s) 353
ZrmI AGTACT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.