Rh4CG110100

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008907
Physical Location & Seq
Forward (+)
20670088 .. 20673179
3092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG110100.1

Sequence Viewer

Length: 1296 bp
ATGGCGAGCAGGTTCCCAAAACCCAGAAGTAGGTACTCTTTGAGTGAAGCAATAATGGGTACAAACAGTACTGGGATTACTACTTATAATGGAGGAGGAGTAGTTCCATACAGTACTGATACTATTAGACCTCATGAGGAACGCAAGAGTATCCAGTTTACTGATTTCCCGACCAATTATGAAGAAATCGTGAAGTTCATCAATGGAAAGCATTCTCCGGAGGACAACACTGACTTTCCTGTTGATGACCTTTTCACGATCACTGAGATCATTATTAAGCATGCAACCGATGATTCGATGGTCAATGATATTCTGAAGGAGACCAATGCCCCATATGAGAAGGTCCCCAGCATCAACGACGGATTCGTCTCGCCATTAAAGAAGCCATACCCTCTATCCGATGTTGTTAAAGAAATCGGGCGCATACGGGAGATCCTTGATGAACTGCATGGAAGATGCGTAGAAGAACTAGAGGTGGCAAAGAACTACAAGAAGCTGCTAAGGAAAATGAAGGACGCCCAACGCACTCGTGAGATTACGGAGGTTATTAAAACCCTGATTCTTTTCAAGGACGGGAAGCTACCTGTCATTAGTGAGGGTAATAGCAACGAGGAGCTTCCATTGATCTCTATAGTTGCGAATATTAACAACACAACTCTAGACGATTGGAGCAAAGATGACACCAAGTGCATTTTCTTATGCGGAGGCATGGATGAGTCGCACTTTGACGATTTGAAAAAGAAAGTGATGGACGCAAGTGATACCATCAAAGATTTCGGTGTGTCGATTGAGTTATTTAGGCTGGACTCACTGGAGGGAAATGAGGCAAAAGCAGGAGAGCAACCAAAATACTCGGCAGATCATGAGAGCTTTTGGTCGGGGATAAAGTGCTTGTTGGATAATTACATACATCGAGTGGAGTGCAAGACCGTGACAAAAGAACTCTGCAAGCTGCTGTCCTACAAAAATCATGGGGAATGGGTTATGGTTAGCCAAGGGTATAAGCTTATAACCAGTGGTCGTCCTGCCACAATTTCTATGGCCCTGGGATATATGTCACAGAGGAAGCAACAAAACTTAGTACTTACAAAATTAGCATTCGGAGATATTTTCCTTCAAGAACATAAGAAGGCTATAACTGTTTATCTGGCTGATAAAGATCAGCATGATCCCGACTGCTGCGAATTTGACATTTCAGTTGGGAATCTCCCCAAGAACATGGACTGCCCAGTCTGCGACCGTTCCATGGAACGCATCTTTATCATCAGCTACAAGTGCTGCCACGGTAGTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

431

Amino Acids

48.89

Weight (kDa)

5.64

Isoelectric Point (pI)

33.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_C PF14577 291 - 428 4.6e-09 Sieve element occlusion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 87, 1010
AasI GACNNNNNNGTC 2 cut(s) 365, 1229
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 1 cut(s) 702
AclWI GGATC 2 cut(s) 427, 1163
AcsI RAATTY 1 cut(s) 1184
AcuI CTGAAG 1 cut(s) 335
AcyI GRCGYC 1 cut(s) 516
AdeI CACNNNGTG 1 cut(s) 687
AfaI GTAC 5 cut(s) 35, 61, 70, 115, 1083
AfiI CCNNNNNNNGG 2 cut(s) 30, 1246
AgsI TTSAA 3 cut(s) 568, 736, 1118
AjnI CCWGG 1 cut(s) 1044
AjuI GAANNNNNNNTTGG 2 cut(s) 677, 709
AloI GAACNNNNNNTCC 2 cut(s) 87, 119
AluBI AGCT 7 cut(s) 496, 580, 616, 870, 952, 1006, 1269
AluI AGCT 7 cut(s) 496, 580, 616, 870, 952, 1006, 1269
Alw26I GTCTC 2 cut(s) 314, 373
AlwI GGATC 2 cut(s) 427, 1163
Aor13HI TCCGGA 1 cut(s) 217
AoxI GGCC 1 cut(s) 1041
ApeKI GCWGC 4 cut(s) 496, 952, 1179, 1278
ApoI RAATTY 1 cut(s) 1184
Asp700I GAANNNNTTC 1 cut(s) 211
AspLEI GCGC 1 cut(s) 423
AspS9I GGNCC 2 cut(s) 343, 1042
AvaII GGWCC 1 cut(s) 343
BauI CACGAG 1 cut(s) 528
BbvI GCAGC 4 cut(s) 483, 939, 1166, 1265
BccI CCATC 3 cut(s) 292, 742, 773
BcgI CGANNNNNNTGC 2 cut(s) 903, 937
BciT130I CCWGG 1 cut(s) 1046
BciVI GTATCC 1 cut(s) 161
BcoDI GTCTC 2 cut(s) 314, 373
BfaI CTAG 2 cut(s) 470, 659
BfmI CTRYAG 1 cut(s) 630
BfuI GTATCC 1 cut(s) 161
BisI GCNGC 4 cut(s) 497, 953, 1180, 1279
BlsI GCNGC 4 cut(s) 498, 954, 1181, 1280
BmcAI AGTACT 3 cut(s) 70, 115, 1083
Bme1390I CCNGG 1 cut(s) 1046
Bme18I GGWCC 1 cut(s) 343
BmgT120I GGNCC 2 cut(s) 343, 1042
BmiI GGNNCC 2 cut(s) 14, 345
BmrFI CCNGG 1 cut(s) 1046
BmrI ACTGGG 2 cut(s) 81, 1223
BmsI GCATC 3 cut(s) 360, 446, 1263
BmuI ACTGGG 2 cut(s) 81, 1223
BpmI CTGGAG 1 cut(s) 833
Bpu10I CCTNAGC 1 cut(s) 500
BsaBI GATNNNNATC 1 cut(s) 1158
BsaHI GRCGYC 1 cut(s) 516
BsaI GGTCTC 1 cut(s) 314
BsaJI CCNNGG 5 cut(s) 994, 1044, 1045, 1245, 1282
BsaWI WCCGGW 1 cut(s) 217
BsaXI ACNNNNNCTCC 4 cut(s) 84, 87, 114, 117
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 1246
Bse1I ACTGG 5 cut(s) 76, 154, 816, 1014, 1229
Bse8I GATNNNNATC 1 cut(s) 1158
BseAI TCCGGA 1 cut(s) 217
BseBI CCWGG 1 cut(s) 1046
BseDI CCNNGG 5 cut(s) 994, 1044, 1045, 1245, 1282
BseGI GGATG 1 cut(s) 718
BseJI GATNNNNATC 1 cut(s) 1158
BseLI CCNNNNNNNGG 2 cut(s) 30, 1246
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 5 cut(s) 76, 154, 816, 1014, 1229
BseRI GAGGAG 3 cut(s) 108, 111, 626
BseXI GCAGC 4 cut(s) 483, 939, 1166, 1265
BseYI CCCAGC 1 cut(s) 347
Bsh1285I CGRYCG 1 cut(s) 1240
BshFI GGCC 1 cut(s) 1043
BsiEI CGRYCG 1 cut(s) 1240
BsiSI CCGG 1 cut(s) 218
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 2 cut(s) 30, 1246
BsmAI GTCTC 2 cut(s) 314, 373
BsmBI CGTCTC 1 cut(s) 373
BsmFI GGGAC 1 cut(s) 329
BsmI GAATGC 2 cut(s) 211, 1097
BsnI GGCC 1 cut(s) 1043
Bso31I GGTCTC 1 cut(s) 314
Bsp13I TCCGGA 1 cut(s) 217
Bsp143I GATC 7 cut(s) 258, 267, 432, 624, 859, 1159, 1168
Bsp19I CCATGG 1 cut(s) 1245
BspACI CCGC 1 cut(s) 702
BspANI GGCC 1 cut(s) 1043
BspCNI CTCAG 1 cut(s) 256
BspEI TCCGGA 1 cut(s) 217
BspHI TCATGA 2 cut(s) 133, 862
BspLI GGNNCC 2 cut(s) 14, 345
BspPI GGATC 2 cut(s) 427, 1163
BspTNI GGTCTC 1 cut(s) 314
BsrI ACTGG 5 cut(s) 76, 154, 816, 1014, 1229
BssECI CCNNGG 5 cut(s) 994, 1044, 1045, 1245, 1282
BssMI GATC 7 cut(s) 258, 267, 432, 624, 859, 1159, 1168
BssNI GRCGYC 1 cut(s) 516
BssSI CACGAG 1 cut(s) 528
BssT1I CCWWGG 2 cut(s) 994, 1245
Bst2BI CACGAG 1 cut(s) 528
Bst2UI CCWGG 1 cut(s) 1046
Bst4CI ACNGT 6 cut(s) 68, 113, 931, 1141, 1241, 1286
BstACI GRCGYC 1 cut(s) 516
BstC8I GCNNGC 3 cut(s) 7, 282, 950
BstDEI CTNAG 3 cut(s) 264, 500, 1078
BstDSI CCRYGG 2 cut(s) 1245, 1282
BstF5I GGATG 1 cut(s) 718
BstHHI GCGC 1 cut(s) 423
BstKTI GATC 7 cut(s) 261, 270, 435, 627, 862, 1162, 1171
BstMAI GTCTC 2 cut(s) 314, 373
BstMBI GATC 7 cut(s) 258, 267, 432, 624, 859, 1159, 1168
BstMCI CGRYCG 1 cut(s) 1240
BstMWI GCNNNNNNNGC 2 cut(s) 1233, 1275
BstNI CCWGG 1 cut(s) 1046
BstNSI RCATGY 1 cut(s) 284
BstSCI CCNGG 1 cut(s) 1044
BstSFI CTRYAG 1 cut(s) 630
BstV1I GCAGC 4 cut(s) 483, 939, 1166, 1265
BstX2I RGATCY 1 cut(s) 432
BstXI CCANNNNNNTGG 1 cut(s) 1219
BstYI RGATCY 1 cut(s) 432
BsuI GTATCC 1 cut(s) 161
BsuRI GGCC 1 cut(s) 1043
BtgI CCRYGG 2 cut(s) 1245, 1282
BtsCI GGATG 1 cut(s) 718
BtsIMutI CAGTG 4 cut(s) 228, 261, 809, 1021
Cac8I GCNNGC 3 cut(s) 7, 282, 950
CciI TCATGA 2 cut(s) 133, 862
CfoI GCGC 1 cut(s) 423
Cfr13I GGNCC 2 cut(s) 343, 1042
CseI GACGC 2 cut(s) 524, 761
Csp6I GTAC 5 cut(s) 34, 60, 69, 114, 1082
CviAII CATG 9 cut(s) 134, 281, 449, 709, 863, 971, 1166, 1219, 1246
CviQI GTAC 5 cut(s) 34, 60, 69, 114, 1082
DdeI CTNAG 3 cut(s) 264, 500, 1078
DpnI GATC 7 cut(s) 260, 269, 434, 626, 861, 1161, 1170
DpnII GATC 7 cut(s) 258, 267, 432, 624, 859, 1159, 1168
DraIII CACNNNGTG 1 cut(s) 687
DrdI GACNNNNNNGTC 2 cut(s) 365, 1229
DseDI GACNNNNNNGTC 2 cut(s) 365, 1229
Eco130I CCWWGG 2 cut(s) 994, 1245
Eco31I GGTCTC 1 cut(s) 314
Eco47I GGWCC 1 cut(s) 343
Eco57I CTGAAG 1 cut(s) 335
EcoO109I RGGNCCY 1 cut(s) 343
EcoRII CCWGG 1 cut(s) 1044
EcoT14I CCWWGG 2 cut(s) 994, 1245
ErhI CCWWGG 2 cut(s) 994, 1245
Esp3I CGTCTC 1 cut(s) 373
FaeI CATG 9 cut(s) 137, 284, 452, 712, 866, 974, 1169, 1222, 1249
FaqI GGGAC 1 cut(s) 329
FatI CATG 9 cut(s) 133, 280, 448, 708, 862, 970, 1165, 1218, 1245
FauNDI CATATG 1 cut(s) 334
Fnu4HI GCNGC 4 cut(s) 497, 953, 1180, 1279
FokI GGATG 1 cut(s) 725
Fsp4HI GCNGC 4 cut(s) 497, 953, 1180, 1279
FspBI CTAG 2 cut(s) 470, 659
GlaI GCGC 1 cut(s) 422
GluI GCNGC 4 cut(s) 497, 953, 1180, 1279
GsaI CCCAGC 1 cut(s) 351
GsuI CTGGAG 1 cut(s) 833
HaeIII GGCC 1 cut(s) 1043
HapII CCGG 1 cut(s) 218
HgaI GACGC 2 cut(s) 524, 761
HhaI GCGC 1 cut(s) 423
Hin1I GRCGYC 1 cut(s) 516
Hin1II CATG 9 cut(s) 137, 284, 452, 712, 866, 974, 1169, 1222, 1249
Hin6I GCGC 1 cut(s) 421
HinP1I GCGC 1 cut(s) 421
HindIII AAGCTT 1 cut(s) 1004
HinfI GANTC 6 cut(s) 293, 363, 559, 716, 806, 1204
HpaII CCGG 1 cut(s) 218
Hpy166II GTNNAC 1 cut(s) 159
Hpy188I TCNGA 3 cut(s) 315, 400, 1103
Hpy8I GTNNAC 1 cut(s) 159
Hpy99I CGWCG 1 cut(s) 362
HpyAV CCTTC 5 cut(s) 310, 334, 505, 1123, 1124
HpyCH4III ACNGT 6 cut(s) 68, 113, 931, 1141, 1241, 1286
HpyCH4V TGCA 5 cut(s) 284, 448, 690, 924, 948
HpyF10VI GCNNNNNNNGC 2 cut(s) 1233, 1275
HpyF3I CTNAG 3 cut(s) 264, 500, 1078
Hsp92I GRCGYC 1 cut(s) 516
Hsp92II CATG 9 cut(s) 137, 284, 452, 712, 866, 974, 1169, 1222, 1249
HspAI GCGC 1 cut(s) 421
Kpn2I TCCGGA 1 cut(s) 217
Kzo9I GATC 7 cut(s) 258, 267, 432, 624, 859, 1159, 1168
LmnI GCTCC 2 cut(s) 613, 669
Lsp1109I GCAGC 4 cut(s) 483, 939, 1166, 1265
LweI GCATC 3 cut(s) 360, 446, 1263
MaeI CTAG 2 cut(s) 470, 659
MaeIII GTNAC 2 cut(s) 931, 1056
MalI GATC 7 cut(s) 260, 269, 434, 626, 861, 1161, 1170
MboI GATC 7 cut(s) 258, 267, 432, 624, 859, 1159, 1168
MboII GAAGA 3 cut(s) 194, 465, 476
MflI RGATCY 1 cut(s) 432
MluCI AATT 5 cut(s) 175, 901, 1032, 1091, 1184
MlyI GAGTC 2 cut(s) 725, 800
MmeI TCCRAC 1 cut(s) 876
MroI TCCGGA 1 cut(s) 217
MroXI GAANNNNTTC 1 cut(s) 211
MseI TTAA 5 cut(s) 276, 377, 408, 549, 645
MspI CCGG 1 cut(s) 218
MspR9I CCNGG 1 cut(s) 1046
Mva1269I GAATGC 2 cut(s) 211, 1097
MvaI CCWGG 1 cut(s) 1046
MwoI GCNNNNNNNGC 2 cut(s) 1233, 1275
NcoI CCATGG 1 cut(s) 1245
NdeI CATATG 1 cut(s) 334
NdeII GATC 7 cut(s) 258, 267, 432, 624, 859, 1159, 1168
NlaIII CATG 9 cut(s) 137, 284, 452, 712, 866, 974, 1169, 1222, 1249
NlaIV GGNNCC 2 cut(s) 14, 345
NmeAIII GCCGAG 1 cut(s) 833
NmuCI GTSAC 2 cut(s) 931, 1056
NspI RCATGY 1 cut(s) 284
PaeI GCATGC 1 cut(s) 284
PagI TCATGA 2 cut(s) 133, 862
PasI CCCWGGG 1 cut(s) 1045
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PctI GAATGC 2 cut(s) 211, 1097
PdmI GAANNNNTTC 1 cut(s) 211
PfeI GAWTC 4 cut(s) 293, 363, 559, 1204
PkrI GCNGC 4 cut(s) 498, 954, 1181, 1280
PleI GAGTC 2 cut(s) 724, 800
PpsI GAGTC 2 cut(s) 724, 800
PpuMI RGGWCCY 1 cut(s) 343
PsiI TTATAA 2 cut(s) 87, 1010
Psp5II RGGWCCY 1 cut(s) 343
Psp6I CCWGG 1 cut(s) 1044
PspFI CCCAGC 1 cut(s) 347
PspGI CCWGG 1 cut(s) 1044
PspN4I GGNNCC 2 cut(s) 14, 345
PspPI GGNCC 2 cut(s) 343, 1042
PspPPI RGGWCCY 1 cut(s) 343
PsuI RGATCY 1 cut(s) 432
RsaI GTAC 5 cut(s) 35, 61, 70, 115, 1083
RsaNI GTAC 5 cut(s) 34, 60, 69, 114, 1082
SaqAI TTAA 5 cut(s) 276, 377, 408, 549, 645
SatI GCNGC 4 cut(s) 497, 953, 1180, 1279
Sau3AI GATC 7 cut(s) 258, 267, 432, 624, 859, 1159, 1168
Sau96I GGNCC 2 cut(s) 343, 1042
ScaI AGTACT 3 cut(s) 70, 115, 1083
SchI GAGTC 2 cut(s) 725, 800
ScrFI CCNGG 1 cut(s) 1046
SfaNI GCATC 3 cut(s) 360, 446, 1263
SfcI CTRYAG 1 cut(s) 630
SinI GGWCC 1 cut(s) 343
SphI GCATGC 1 cut(s) 284
Sse9I AATT 5 cut(s) 175, 901, 1032, 1091, 1184
SsiI CCGC 1 cut(s) 702
SspI AATATT 1 cut(s) 643
SspMI CTAG 2 cut(s) 470, 659
StyD4I CCNGG 1 cut(s) 1044
StyI CCWWGG 2 cut(s) 994, 1245
TaaI ACNGT 6 cut(s) 68, 113, 931, 1141, 1241, 1286
TaqI TCGA 3 cut(s) 296, 785, 913
TasI AATT 5 cut(s) 175, 901, 1032, 1091, 1184
TatI WGTACW 3 cut(s) 68, 113, 1081
TfiI GAWTC 4 cut(s) 293, 363, 559, 1204
Tru1I TTAA 5 cut(s) 276, 377, 408, 549, 645
Tru9I TTAA 5 cut(s) 276, 377, 408, 549, 645
TscAI CASTG 4 cut(s) 235, 268, 816, 1021
TseFI GTSAC 2 cut(s) 931, 1056
TseI GCWGC 4 cut(s) 496, 952, 1179, 1278
Tsp45I GTSAC 2 cut(s) 931, 1056
TspDTI ATGAA 4 cut(s) 187, 195, 456, 524
TspGWI ACGGA 2 cut(s) 375, 554
TspRI CASTG 4 cut(s) 235, 268, 816, 1021
VpaK11BI GGWCC 1 cut(s) 343
XapI RAATTY 1 cut(s) 1184
XbaI TCTAGA 1 cut(s) 658
XceI RCATGY 1 cut(s) 284
XcmI CCANNNNNNNNNTGG 1 cut(s) 1036
XmnI GAANNNNTTC 1 cut(s) 211
XspI CTAG 2 cut(s) 470, 659
ZrmI AGTACT 3 cut(s) 70, 115, 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.