Rw4G008280

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0008907
Physical Location & Seq
Forward (+)
18007590 .. 18009352
1763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G008280.1

Sequence Viewer

Length: 1281 bp
ATGCCACACGATCTACCCGATGTTGTTCCAAAAATCCAGCGCATACGGGAGAAACTTAACAAACTGCATGGAACATGCGTAAAAGAAATAGGTGTTATACTTGATGTGGCAAAGAGCTGCAAGAGGCTGCACAGGAAAATGGTGGACGCCCAACGAACTCTTAAGATTACGGAGGTTATTAACACCCTGATTCTTTTCACGGATAACGGGAAGCTACCTGTCATTAGTGTGGGTAGCAACGAGGAGGTTAAGTCTGAAACATTTGCCGACTTGTTAAAGGAAAACTCTGTGTTGTTTTACATTTCGAGCCTGGAGAACATTACGGACGATGACATTTCCGATCTGATACAAGTTTATGATGAAATTCAAAAAACTAATAGAAAATGGAGGATGGTGTGGATCCCCATTGTGGAAGACTGGACGAAAAACCAGAATGAGAAGTTTGAGAAATGGGGATCGAAGATGCCATGGTACAAGGTGCGGTTTCTTTCTTCAATAGTCATCAAGTACCTCGAGCAGGAATGGTCCTACACGGGTATTACAATGTCCATGGTGATGGACCCACGAGGGCAGAGGCAATTCCACAATGCACTCGACTTGATCCGGAGACATAAAAACAAATTCTTTGATATCCTTGACCTGAACGTTTCAGTCATCGAGTCTATATTTGGGAATATGAAGAATAAAAAGCTAGAAGAGTGGAGCAAAGATGACACTAAGTGCATTTTCTTATACGGAGACATGGATGAGTCGTGGATTAACAAGTTGAAAAAGAAAGTGGAGGAAGCAAAGGATACCATCAAAGATTTCGGTATGTCGATTGAGTTACATAGGGTGGAGGGAAACGAAGCAAAAGTACTTTGGTCGGGGATAGGGAGCTTGTTGACTGATTACCTAGGTCAAGTGGAGTACAAGACCGTGAAAAAGGAACTCTACCAGCTACTGTCCTACAAAAATCATGGGGAATGGGTTATAGTTAGCCAAGGGTATGATCTTATAACCAGTGGTCATCCTGCCACAATTTGTGATGTCCTAAAAAATATGTCACAGTGGAAGCAAGATGAGTCGAAGACATCACGTAAAACATCCACATTCGGAAATTATTTCCAAGAAGACCATAAGAACACAACTCAAATTAGTGATCACAAGTACTGCTGCGAATTTGACATTTCCGGGAATCCCCCCAAGAACTTGGACTGCCCAGTCTGCGGCCGTTCCATGAAAACCACCCCTATCATCAGGTACAAGTGCTGCCACGGTGCCACTGAAACGAGTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

426

Amino Acids

49.55

Weight (kDa)

7.92

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_C PF14577 301 - 419 2.2e-11 Sieve element occlusion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 998
AasI GACNNNNNNGTC 1 cut(s) 1202
AccB1I GGYRCC 1 cut(s) 1259
AccIII TCCGGA 1 cut(s) 603
AciI CCGC 2 cut(s) 481, 1209
AclI AACGTT 1 cut(s) 645
AclWI GGATC 4 cut(s) 394, 407, 463, 595
AcoI YGGCCR 1 cut(s) 1210
AcsI RAATTY 3 cut(s) 363, 620, 1160
AcyI GRCGYC 1 cut(s) 147
AdeI CACNNNGTG 1 cut(s) 720
AfaI GTAC 6 cut(s) 473, 509, 858, 911, 1151, 1244
AfiI CCNNNNNNNGG 3 cut(s) 409, 517, 1208
AflII CTTAAG 1 cut(s) 161
AgsI TTSAA 3 cut(s) 368, 495, 769
AjnI CCWGG 1 cut(s) 309
AluBI AGCT 5 cut(s) 117, 214, 691, 879, 940
AluI AGCT 5 cut(s) 117, 214, 691, 879, 940
Alw26I GTCTC 2 cut(s) 601, 732
AlwI GGATC 4 cut(s) 394, 407, 463, 595
AlwNI CAGNNNCTG 1 cut(s) 943
Ama87I CYCGRG 1 cut(s) 512
Aor13HI TCCGGA 1 cut(s) 603
AoxI GGCC 1 cut(s) 1210
ApeKI GCWGC 4 cut(s) 117, 127, 1155, 1251
ApoI RAATTY 3 cut(s) 363, 620, 1160
AspA2I CCTAGG 1 cut(s) 895
AspLEI GCGC 1 cut(s) 42
AspS9I GGNCC 2 cut(s) 525, 559
AsuC2I CCSGG 1 cut(s) 1174
AsuHPI GGTGA 1 cut(s) 565
AvaI CYCGRG 1 cut(s) 512
AvaII GGWCC 2 cut(s) 525, 559
AvrII CCTAGG 1 cut(s) 895
BamHI GGATCC 1 cut(s) 399
BanI GGYRCC 1 cut(s) 1259
BarI GAAGNNNNNNTAC 2 cut(s) 840, 872
BauI CACGAG 1 cut(s) 564
BbsI GAAGAC 3 cut(s) 420, 1076, 1119
BbvI GCAGC 4 cut(s) 104, 114, 1142, 1238
BccI CCATC 3 cut(s) 385, 550, 806
BceAI ACGGC 1 cut(s) 1197
BciT130I CCWGG 1 cut(s) 311
BciVI GTATCC 1 cut(s) 787
BclI TGATCA 1 cut(s) 1141
BcnI CCSGG 1 cut(s) 1174
BcoDI GTCTC 2 cut(s) 601, 732
BfaI CTAG 2 cut(s) 692, 896
BfrI CTTAAG 1 cut(s) 161
BfuI GTATCC 1 cut(s) 787
BisI GCNGC 5 cut(s) 118, 128, 1156, 1210, 1252
BlnI CCTAGG 1 cut(s) 895
BlsI GCNGC 5 cut(s) 119, 129, 1157, 1211, 1253
BmcAI AGTACT 2 cut(s) 858, 1151
Bme1390I CCNGG 2 cut(s) 311, 1174
Bme18I GGWCC 2 cut(s) 525, 559
BmeT110I CYCGRG 1 cut(s) 512
BmgT120I GGNCC 2 cut(s) 525, 559
BmiI GGNNCC 3 cut(s) 401, 561, 1261
BmrFI CCNGG 2 cut(s) 311, 1174
BmrI ACTGGG 1 cut(s) 1196
BmsI GCATC 1 cut(s) 453
BmuI ACTGGG 1 cut(s) 1196
BpiI GAAGAC 3 cut(s) 420, 1076, 1119
BpmI CTGGAG 1 cut(s) 332
BpuMI CCSGG 1 cut(s) 1174
BsaAI YACGTR 1 cut(s) 1079
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 5 cut(s) 467, 549, 895, 982, 1255
BsaWI WCCGGW 1 cut(s) 603
BsaXI ACNNNNNCTCC 2 cut(s) 236, 266
Bsc4I CCNNNNNNNGG 3 cut(s) 409, 517, 1208
Bse1I ACTGG 3 cut(s) 422, 1002, 1202
BseAI TCCGGA 1 cut(s) 603
BseBI CCWGG 1 cut(s) 311
BseDI CCNNGG 5 cut(s) 467, 549, 895, 982, 1255
BseGI GGATG 4 cut(s) 396, 751, 1009, 1085
BseLI CCNNNNNNNGG 3 cut(s) 409, 517, 1208
BseNI ACTGG 3 cut(s) 422, 1002, 1202
BseRI GAGGAG 1 cut(s) 257
BseX3I CGGCCG 1 cut(s) 1210
BseXI GCAGC 4 cut(s) 104, 114, 1142, 1238
BsgI GTGCAG 1 cut(s) 113
Bsh1285I CGRYCG 1 cut(s) 1213
BshFI GGCC 1 cut(s) 1212
BshNI GGYRCC 1 cut(s) 1259
BsiEI CGRYCG 1 cut(s) 1213
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 2 cut(s) 604, 1173
BslI CCNNNNNNNGG 3 cut(s) 409, 517, 1208
BsmAI GTCTC 2 cut(s) 601, 732
BsnI GGCC 1 cut(s) 1212
BsoBI CYCGRG 1 cut(s) 512
Bsp13I TCCGGA 1 cut(s) 603
Bsp143I GATC 7 cut(s) 10, 340, 399, 455, 600, 991, 1141
Bsp19I CCATGG 2 cut(s) 467, 549
BspACI CCGC 2 cut(s) 481, 1209
BspANI GGCC 1 cut(s) 1212
BspEI TCCGGA 1 cut(s) 603
BspLI GGNNCC 3 cut(s) 401, 561, 1261
BspPI GGATC 4 cut(s) 394, 407, 463, 595
BspT107I GGYRCC 1 cut(s) 1259
BspTI CTTAAG 1 cut(s) 161
BsrI ACTGG 3 cut(s) 422, 1002, 1202
BssECI CCNNGG 5 cut(s) 467, 549, 895, 982, 1255
BssMI GATC 7 cut(s) 10, 340, 399, 455, 600, 991, 1141
BssNI GRCGYC 1 cut(s) 147
BssSI CACGAG 1 cut(s) 564
BssT1I CCWWGG 4 cut(s) 467, 549, 895, 982
Bst2BI CACGAG 1 cut(s) 564
Bst2UI CCWGG 1 cut(s) 311
Bst4CI ACNGT 4 cut(s) 919, 945, 1050, 1259
Bst6I CTCTTC 1 cut(s) 690
BstACI GRCGYC 1 cut(s) 147
BstAFI CTTAAG 1 cut(s) 161
BstBAI YACGTR 1 cut(s) 1079
BstDEI CTNAG 1 cut(s) 717
BstDSI CCRYGG 3 cut(s) 467, 549, 1255
BstENI CCTNNNNNAGG 1 cut(s) 515
BstF5I GGATG 4 cut(s) 396, 751, 1009, 1085
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 7 cut(s) 13, 343, 402, 458, 603, 994, 1144
BstMAI GTCTC 2 cut(s) 601, 732
BstMBI GATC 7 cut(s) 10, 340, 399, 455, 600, 991, 1141
BstMCI CGRYCG 1 cut(s) 1213
BstMWI GCNNNNNNNGC 1 cut(s) 1206
BstNI CCWGG 1 cut(s) 311
BstNSI RCATGY 1 cut(s) 78
BstSCI CCNGG 2 cut(s) 309, 1172
BstV1I GCAGC 4 cut(s) 104, 114, 1142, 1238
BstV2I GAAGAC 3 cut(s) 420, 1076, 1119
BstX2I RGATCY 1 cut(s) 399
BstXI CCANNNNNNTGG 2 cut(s) 556, 1192
BstYI RGATCY 1 cut(s) 399
BstZI CGGCCG 1 cut(s) 1210
BsuI GTATCC 1 cut(s) 787
BsuRI GGCC 1 cut(s) 1212
BtgI CCRYGG 3 cut(s) 467, 549, 1255
BtsCI GGATG 4 cut(s) 396, 751, 1009, 1085
BtsIMutI CAGTG 3 cut(s) 1009, 1055, 1263
CaiI CAGNNNCTG 1 cut(s) 943
CfoI GCGC 1 cut(s) 42
Cfr13I GGNCC 2 cut(s) 525, 559
CseI GACGC 1 cut(s) 155
Csp6I GTAC 6 cut(s) 472, 508, 857, 910, 1150, 1243
CviAII CATG 7 cut(s) 68, 75, 468, 550, 742, 959, 1219
CviJI RGCY 9 cut(s) 117, 127, 214, 309, 691, 879, 940, 981, 1212
CviKI_1 RGCY 9 cut(s) 117, 127, 214, 309, 691, 879, 940, 981, 1212
CviQI GTAC 6 cut(s) 472, 508, 857, 910, 1150, 1243
DdeI CTNAG 1 cut(s) 717
DpnI GATC 7 cut(s) 12, 342, 401, 457, 602, 993, 1143
DpnII GATC 7 cut(s) 10, 340, 399, 455, 600, 991, 1141
DraIII CACNNNGTG 1 cut(s) 720
DrdI GACNNNNNNGTC 1 cut(s) 1202
DseDI GACNNNNNNGTC 1 cut(s) 1202
EaeI YGGCCR 1 cut(s) 1210
EagI CGGCCG 1 cut(s) 1210
Eam1104I CTCTTC 1 cut(s) 690
EarI CTCTTC 1 cut(s) 690
EclXI CGGCCG 1 cut(s) 1210
Eco130I CCWWGG 4 cut(s) 467, 549, 895, 982
Eco32I GATATC 1 cut(s) 631
Eco47I GGWCC 2 cut(s) 525, 559
Eco52I CGGCCG 1 cut(s) 1210
Eco88I CYCGRG 1 cut(s) 512
EcoNI CCTNNNNNAGG 1 cut(s) 515
EcoRII CCWGG 1 cut(s) 309
EcoRV GATATC 1 cut(s) 631
EcoT14I CCWWGG 4 cut(s) 467, 549, 895, 982
ErhI CCWWGG 4 cut(s) 467, 549, 895, 982
FaeI CATG 7 cut(s) 71, 78, 471, 553, 745, 962, 1222
FatI CATG 7 cut(s) 67, 74, 467, 549, 741, 958, 1218
FbaI TGATCA 1 cut(s) 1141
Fnu4HI GCNGC 5 cut(s) 118, 128, 1156, 1210, 1252
FokI GGATG 4 cut(s) 403, 758, 996, 1072
Fsp4HI GCNGC 5 cut(s) 118, 128, 1156, 1210, 1252
FspBI CTAG 2 cut(s) 692, 896
GlaI GCGC 1 cut(s) 41
GluI GCNGC 5 cut(s) 118, 128, 1156, 1210, 1252
GsuI CTGGAG 1 cut(s) 332
HaeIII GGCC 1 cut(s) 1212
HapII CCGG 2 cut(s) 604, 1173
HgaI GACGC 1 cut(s) 155
HhaI GCGC 1 cut(s) 42
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 7 cut(s) 71, 78, 471, 553, 745, 962, 1222
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HincII GTYRAC 1 cut(s) 885
HindII GTYRAC 1 cut(s) 885
HinfI GANTC 6 cut(s) 190, 659, 749, 1064, 1177, 1273
HpaII CCGG 2 cut(s) 604, 1173
HphI GGTGA 1 cut(s) 565
Hpy166II GTNNAC 2 cut(s) 145, 885
Hpy188I TCNGA 4 cut(s) 256, 340, 345, 1097
Hpy188III TCNNGA 1 cut(s) 604
Hpy8I GTNNAC 2 cut(s) 145, 885
HpyCH4III ACNGT 4 cut(s) 919, 945, 1050, 1259
HpyCH4IV ACGT 2 cut(s) 645, 1078
HpyCH4V TGCA 5 cut(s) 67, 120, 130, 590, 723
HpyF10VI GCNNNNNNNGC 1 cut(s) 1206
HpyF3I CTNAG 1 cut(s) 717
HpySE526I ACGT 2 cut(s) 645, 1078
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 7 cut(s) 71, 78, 471, 553, 745, 962, 1222
HspAI GCGC 1 cut(s) 40
Kpn2I TCCGGA 1 cut(s) 603
Ksp22I TGATCA 1 cut(s) 1141
Kzo9I GATC 7 cut(s) 10, 340, 399, 455, 600, 991, 1141
LmnI GCTCC 2 cut(s) 702, 876
Lsp1109I GCAGC 4 cut(s) 104, 114, 1142, 1238
LweI GCATC 1 cut(s) 453
MaeI CTAG 2 cut(s) 692, 896
MaeII ACGT 2 cut(s) 645, 1078
MaeIII GTNAC 2 cut(s) 825, 1044
MalI GATC 7 cut(s) 12, 342, 401, 457, 602, 993, 1143
MboI GATC 7 cut(s) 10, 340, 399, 455, 600, 991, 1141
MboII GAAGA 7 cut(s) 425, 472, 483, 691, 707, 1081, 1124
MflI RGATCY 1 cut(s) 399
MluCI AATT 7 cut(s) 363, 578, 620, 1020, 1099, 1134, 1160
MlyI GAGTC 3 cut(s) 668, 758, 1073
MroI TCCGGA 1 cut(s) 603
MseI TTAA 7 cut(s) 57, 162, 180, 249, 275, 759, 1279
MslI CAYNNNNRTG 2 cut(s) 227, 554
MspCI CTTAAG 1 cut(s) 161
MspI CCGG 2 cut(s) 604, 1173
MspR9I CCNGG 2 cut(s) 311, 1174
MvaI CCWGG 1 cut(s) 311
MwoI GCNNNNNNNGC 1 cut(s) 1206
NciI CCSGG 1 cut(s) 1174
NcoI CCATGG 2 cut(s) 467, 549
NdeII GATC 7 cut(s) 10, 340, 399, 455, 600, 991, 1141
NlaIII CATG 7 cut(s) 71, 78, 471, 553, 745, 962, 1222
NlaIV GGNNCC 3 cut(s) 401, 561, 1261
NmuCI GTSAC 1 cut(s) 1044
NspI RCATGY 1 cut(s) 78
PaeR7I CTCGAG 1 cut(s) 512
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PfeI GAWTC 2 cut(s) 190, 1177
PfoI TCCNGGA 1 cut(s) 1172
PkrI GCNGC 5 cut(s) 119, 129, 1157, 1211, 1253
PleI GAGTC 3 cut(s) 667, 757, 1072
PpsI GAGTC 3 cut(s) 667, 757, 1072
Ppu21I YACGTR 1 cut(s) 1079
PsiI TTATAA 1 cut(s) 998
Psp1406I AACGTT 1 cut(s) 645
Psp6I CCWGG 1 cut(s) 309
PspGI CCWGG 1 cut(s) 309
PspN4I GGNNCC 3 cut(s) 401, 561, 1261
PspPI GGNCC 2 cut(s) 525, 559
PspXI VCTCGAGB 1 cut(s) 512
PstNI CAGNNNCTG 1 cut(s) 943
PsuI RGATCY 1 cut(s) 399
RsaI GTAC 6 cut(s) 473, 509, 858, 911, 1151, 1244
RsaNI GTAC 6 cut(s) 472, 508, 857, 910, 1150, 1243
RseI CAYNNNNRTG 2 cut(s) 227, 554
SaqAI TTAA 7 cut(s) 57, 162, 180, 249, 275, 759, 1279
SatI GCNGC 5 cut(s) 118, 128, 1156, 1210, 1252
Sau3AI GATC 7 cut(s) 10, 340, 399, 455, 600, 991, 1141
Sau96I GGNCC 2 cut(s) 525, 559
ScaI AGTACT 2 cut(s) 858, 1151
SchI GAGTC 3 cut(s) 668, 758, 1073
ScrFI CCNGG 2 cut(s) 311, 1174
SfaNI GCATC 1 cut(s) 453
Sfr274I CTCGAG 1 cut(s) 512
SinI GGWCC 2 cut(s) 525, 559
SlaI CTCGAG 1 cut(s) 512
SmiMI CAYNNNNRTG 2 cut(s) 227, 554
SmlI CTYRAG 2 cut(s) 161, 512
SmoI CTYRAG 2 cut(s) 161, 512
Sse9I AATT 7 cut(s) 363, 578, 620, 1020, 1099, 1134, 1160
SsiI CCGC 2 cut(s) 481, 1209
SspMI CTAG 2 cut(s) 692, 896
StyD4I CCNGG 2 cut(s) 309, 1172
StyI CCWWGG 4 cut(s) 467, 549, 895, 982
TaaI ACNGT 4 cut(s) 919, 945, 1050, 1259
TaiI ACGT 2 cut(s) 648, 1081
TaqI TCGA 7 cut(s) 305, 458, 513, 594, 657, 818, 1067
TasI AATT 7 cut(s) 363, 578, 620, 1020, 1099, 1134, 1160
TatI WGTACW 3 cut(s) 856, 909, 1149
TauI GCSGC 1 cut(s) 1212
TfiI GAWTC 2 cut(s) 190, 1177
Tru1I TTAA 7 cut(s) 57, 162, 180, 249, 275, 759, 1279
Tru9I TTAA 7 cut(s) 57, 162, 180, 249, 275, 759, 1279
TscAI CASTG 3 cut(s) 1009, 1055, 1270
TseFI GTSAC 1 cut(s) 1044
TseI GCWGC 4 cut(s) 117, 127, 1155, 1251
Tsp45I GTSAC 1 cut(s) 1044
TspDTI ATGAA 3 cut(s) 375, 692, 1235
TspGWI ACGGA 4 cut(s) 185, 215, 338, 750
TspRI CASTG 3 cut(s) 1009, 1055, 1270
Vha464I CTTAAG 1 cut(s) 161
VpaK11BI GGWCC 2 cut(s) 525, 559
XagI CCTNNNNNAGG 1 cut(s) 515
XapI RAATTY 3 cut(s) 363, 620, 1160
XceI RCATGY 1 cut(s) 78
XhoI CTCGAG 1 cut(s) 512
XmaJI CCTAGG 1 cut(s) 895
XspI CTAG 2 cut(s) 692, 896
ZrmI AGTACT 2 cut(s) 858, 1151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.