Rh4CG033300

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008907
Physical Location & Seq
Forward (+)
6715961 .. 6716814
854 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG033300.1

Sequence Viewer

Length: 567 bp
ATGGCAAGCAAGCTCACTGAAATGTCAGACGAAGAAATCCTGAACTTCATCAATGGAAAGTATTCTCTGGGGGACAGCACTGCCTTTGATGTTGGTGATCTTTTCACGATCACTAAGACCATCATTGATCATGCAACCCGTAATTCGATGGTCGATGATATTCTGAAGTCGGCCAATGCCCTGAATGAGAAGGTCCCCAACATCAACGACAGCTTCATCTCGCCATTGTGCACTCTTAAGTCCATTTACTGCGAGATGTCATCATGTATGGATAAGGCTTGTTCTGGTGAAGAAATTTCCATCCAACCAAGGACGGAAGTGATATTTGACAAGCTAAAACCCTATCCATGGGACGCTAAGGCAGTGCTGGCCCTAGCGGCTTTCGCTTTGGAATACGGAGACTTCTGGCACCTCGCCCAGCTCTATGGCCAATGTAACCAACTCACCAATTCACTGGCTATCCTGAAGCGAGTACCCGTCCTTACCAAGCATGCGACCTTGGAGAAACGCAAGGAGGAAGTACATGGTTCAGCTCAACTCATCAAAGCCAGTGGCGGAACTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

188

Amino Acids

20.75

Weight (kDa)

5.38

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 10 - 183 8.8e-32 Sieve element occlusion N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 408
AciI CCGC 2 cut(s) 377, 555
AcoI YGGCCR 2 cut(s) 171, 427
AcsI RAATTY 1 cut(s) 294
AcuI CTGAAG 2 cut(s) 185, 485
AfaI GTAC 2 cut(s) 474, 522
AfiI CCNNNNNNNGG 1 cut(s) 348
AflII CTTAAG 1 cut(s) 236
AluBI AGCT 5 cut(s) 13, 213, 334, 421, 533
AluI AGCT 5 cut(s) 13, 213, 334, 421, 533
Alw21I GWGCWC 1 cut(s) 233
Alw26I GTCTC 1 cut(s) 393
Alw44I GTGCAC 1 cut(s) 229
AoxI GGCC 3 cut(s) 171, 369, 427
ApaLI GTGCAC 1 cut(s) 229
ApoI RAATTY 1 cut(s) 294
Asp700I GAANNNNTTC 1 cut(s) 61
AspS9I GGNCC 2 cut(s) 193, 370
AsuHPI GGTGA 3 cut(s) 107, 299, 436
AvaII GGWCC 1 cut(s) 193
BaeGI GKGCMC 1 cut(s) 233
BalI TGGCCA 1 cut(s) 429
BanI GGYRCC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 233
BccI CCATC 3 cut(s) 128, 142, 308
BclI TGATCA 1 cut(s) 127
BcoDI GTCTC 1 cut(s) 393
BfaI CTAG 1 cut(s) 374
BfrI CTTAAG 1 cut(s) 236
BglI GCCNNNNNGGC 1 cut(s) 377
BisI GCNGC 1 cut(s) 378
BlsI GCNGC 1 cut(s) 379
Bme18I GGWCC 1 cut(s) 193
BmgT120I GGNCC 2 cut(s) 193, 370
BmiI GGNNCC 2 cut(s) 195, 410
Bpu10I CCTNAGC 1 cut(s) 357
BsaJI CCNNGG 3 cut(s) 308, 347, 498
Bsc4I CCNNNNNNNGG 1 cut(s) 348
Bse1I ACTGG 2 cut(s) 459, 549
BseDI CCNNGG 3 cut(s) 308, 347, 498
BseGI GGATG 1 cut(s) 300
BseLI CCNNNNNNNGG 1 cut(s) 348
BseNI ACTGG 2 cut(s) 459, 549
BseSI GKGCMC 1 cut(s) 233
BseYI CCCAGC 1 cut(s) 417
BshFI GGCC 3 cut(s) 173, 371, 429
BshNI GGYRCC 1 cut(s) 408
BsiHKAI GWGCWC 1 cut(s) 233
BslFI GGGAC 3 cut(s) 86, 179, 365
BslI CCNNNNNNNGG 1 cut(s) 348
BsmAI GTCTC 1 cut(s) 393
BsmFI GGGAC 3 cut(s) 86, 179, 365
BsnI GGCC 3 cut(s) 173, 371, 429
Bsp1286I GDGCHC 1 cut(s) 233
Bsp143I GATC 3 cut(s) 97, 108, 127
Bsp19I CCATGG 1 cut(s) 347
BspACI CCGC 2 cut(s) 377, 555
BspANI GGCC 3 cut(s) 173, 371, 429
BspLI GGNNCC 2 cut(s) 195, 410
BspT107I GGYRCC 1 cut(s) 408
BspTI CTTAAG 1 cut(s) 236
BsrI ACTGG 2 cut(s) 459, 549
BssECI CCNNGG 3 cut(s) 308, 347, 498
BssMI GATC 3 cut(s) 97, 108, 127
BssT1I CCWWGG 3 cut(s) 308, 347, 498
Bst4CI ACNGT 1 cut(s) 562
BstAFI CTTAAG 1 cut(s) 236
BstC8I GCNNGC 4 cut(s) 7, 11, 369, 492
BstDEI CTNAG 2 cut(s) 114, 357
BstDSI CCRYGG 1 cut(s) 347
BstF5I GGATG 1 cut(s) 300
BstKTI GATC 3 cut(s) 100, 111, 130
BstMAI GTCTC 1 cut(s) 393
BstMBI GATC 3 cut(s) 97, 108, 127
BstMWI GCNNNNNNNGC 3 cut(s) 368, 377, 383
BstNSI RCATGY 1 cut(s) 494
BstSLI GKGCMC 1 cut(s) 233
BstXI CCANNNNNNTGG 2 cut(s) 425, 454
BsuRI GGCC 3 cut(s) 173, 371, 429
BtgI CCRYGG 1 cut(s) 347
BtsCI GGATG 1 cut(s) 300
BtsI GCAGTG 2 cut(s) 78, 369
BtsIMutI CAGTG 5 cut(s) 15, 78, 369, 452, 556
Cac8I GCNNGC 4 cut(s) 7, 11, 369, 492
Cfr13I GGNCC 2 cut(s) 193, 370
CseI GACGC 1 cut(s) 362
Csp6I GTAC 2 cut(s) 473, 521
CspCI CAANNNNNGTGG 2 cut(s) 532, 567
CviAII CATG 5 cut(s) 131, 264, 348, 491, 524
CviQI GTAC 2 cut(s) 473, 521
DdeI CTNAG 2 cut(s) 114, 357
DpnI GATC 3 cut(s) 99, 110, 129
DpnII GATC 3 cut(s) 97, 108, 127
EaeI YGGCCR 2 cut(s) 171, 427
Eco130I CCWWGG 3 cut(s) 308, 347, 498
Eco47I GGWCC 1 cut(s) 193
Eco57I CTGAAG 2 cut(s) 185, 485
EcoO109I RGGNCCY 1 cut(s) 193
EcoT14I CCWWGG 3 cut(s) 308, 347, 498
ErhI CCWWGG 3 cut(s) 308, 347, 498
FaeI CATG 5 cut(s) 134, 267, 351, 494, 527
FaiI YATR 7 cut(s) 132, 265, 269, 349, 426, 492, 525
FaqI GGGAC 3 cut(s) 86, 179, 365
FatI CATG 5 cut(s) 130, 263, 347, 490, 523
FbaI TGATCA 1 cut(s) 127
Fnu4HI GCNGC 1 cut(s) 378
FokI GGATG 1 cut(s) 287
Fsp4HI GCNGC 1 cut(s) 378
FspBI CTAG 1 cut(s) 374
GluI GCNGC 1 cut(s) 378
GsaI CCCAGC 1 cut(s) 421
HaeIII GGCC 3 cut(s) 173, 371, 429
HgaI GACGC 1 cut(s) 362
Hin1II CATG 5 cut(s) 134, 267, 351, 494, 527
HphI GGTGA 3 cut(s) 107, 299, 436
Hpy166II GTNNAC 1 cut(s) 231
Hpy188I TCNGA 2 cut(s) 28, 165
Hpy188III TCNNGA 3 cut(s) 40, 106, 463
Hpy8I GTNNAC 1 cut(s) 231
HpyAV CCTTC 1 cut(s) 184
HpyCH4III ACNGT 1 cut(s) 562
HpyCH4V TGCA 2 cut(s) 134, 231
HpyF10VI GCNNNNNNNGC 3 cut(s) 368, 377, 383
HpyF3I CTNAG 2 cut(s) 114, 357
Hsp92II CATG 5 cut(s) 134, 267, 351, 494, 527
Ksp22I TGATCA 1 cut(s) 127
Kzo9I GATC 3 cut(s) 97, 108, 127
MaeI CTAG 1 cut(s) 374
MaeIII GTNAC 1 cut(s) 434
MalI GATC 3 cut(s) 99, 110, 129
MboI GATC 3 cut(s) 97, 108, 127
MboII GAAGA 2 cut(s) 44, 302
MhlI GDGCHC 1 cut(s) 233
MlsI TGGCCA 1 cut(s) 429
MluCI AATT 3 cut(s) 142, 294, 448
MluNI TGGCCA 1 cut(s) 429
MmeI TCCRAC 1 cut(s) 328
MnlI CCTC 2 cut(s) 422, 508
Mox20I TGGCCA 1 cut(s) 429
MroXI GAANNNNTTC 1 cut(s) 61
MscI TGGCCA 1 cut(s) 429
MseI TTAA 1 cut(s) 237
MslI CAYNNNNRTG 1 cut(s) 20
Msp20I TGGCCA 1 cut(s) 429
MspCI CTTAAG 1 cut(s) 236
MwoI GCNNNNNNNGC 3 cut(s) 368, 377, 383
NcoI CCATGG 1 cut(s) 347
NdeII GATC 3 cut(s) 97, 108, 127
NlaIII CATG 5 cut(s) 134, 267, 351, 494, 527
NlaIV GGNNCC 2 cut(s) 195, 410
NspI RCATGY 1 cut(s) 494
PaeI GCATGC 1 cut(s) 494
PdmI GAANNNNTTC 1 cut(s) 61
PkrI GCNGC 1 cut(s) 379
PpuMI RGGWCCY 1 cut(s) 193
Psp5II RGGWCCY 1 cut(s) 193
PspFI CCCAGC 1 cut(s) 417
PspN4I GGNNCC 2 cut(s) 195, 410
PspPI GGNCC 2 cut(s) 193, 370
PspPPI RGGWCCY 1 cut(s) 193
RsaI GTAC 2 cut(s) 474, 522
RsaNI GTAC 2 cut(s) 473, 521
RseI CAYNNNNRTG 1 cut(s) 20
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 1 cut(s) 378
Sau3AI GATC 3 cut(s) 97, 108, 127
Sau96I GGNCC 2 cut(s) 193, 370
SduI GDGCHC 1 cut(s) 233
SetI ASST 8 cut(s) 15, 195, 215, 336, 414, 423, 500, 535
SinI GGWCC 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 20
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
SphI GCATGC 1 cut(s) 494
Sse9I AATT 3 cut(s) 142, 294, 448
SsiI CCGC 2 cut(s) 377, 555
SspMI CTAG 1 cut(s) 374
StyI CCWWGG 3 cut(s) 308, 347, 498
TaaI ACNGT 1 cut(s) 562
TaqI TCGA 2 cut(s) 146, 153
TasI AATT 3 cut(s) 142, 294, 448
TatI WGTACW 1 cut(s) 520
TauI GCSGC 1 cut(s) 380
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 5 cut(s) 22, 85, 369, 459, 556
TspDTI ATGAA 2 cut(s) 37, 205
TspGWI ACGGA 2 cut(s) 329, 411
TspRI CASTG 5 cut(s) 22, 85, 369, 459, 556
Vha464I CTTAAG 1 cut(s) 236
VneI GTGCAC 1 cut(s) 229
VpaK11BI GGWCC 1 cut(s) 193
XapI RAATTY 1 cut(s) 294
XceI RCATGY 1 cut(s) 494
XmnI GAANNNNTTC 1 cut(s) 61
XspI CTAG 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.