Rmu_sc0003822.1_g000027

starch synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003822.1
OG0009675
Physical Location & Seq
Forward (+)
103773 .. 115465
11693 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1845 bp
ctgtacaagcttacctcgaccgcccagacgctgacgactccctctccggcgttcaaccaccgacccgagccagtcgattgcatcccagcagcagcgaatcatccccgtcgtccgcttgcagaggaaggtatcggcggtcaggcgggttcgggttcgagcagtcaggcgggttcaattggcctttttgatgcgcgcaccgaggtcggcattgcaaagccaacaaatttgactgattctgatattcagggatttgtttggccttctccaacagatgaaatcccattttggaagaaggagttcccttcttgggatgcaaacttggaacttccctctgaacaaaccaaggattccaaactcatgcacattgttcatgttacagctgaaatggcaccaatagcaaaagttggtggtcttggtgatgttgtaactggacttgctcgagcatgtttatcacgtggccacacagtccatataatgcttcctttctatgagtgtatccagaaacatcaaatcaatgacttgacgttaatcacaacatataactcttatcatgatggaaactggatccccactaatgcatatcatggggtagtttcaggaatcccagttatatttcttgaaccatcctgtcagttctttaagggacgtcaggtgtatgggggttcatacaatgagctggaggcatatatgttctttagtcgtgcttgccttgaatggatgcaggtgactggaacacaacctgatatcattcatgtccatgagtggcaggctggtgctttacccatactttattgggacatgtatcatcatctttcacttaagaagccgagaatagtattgactatccataacatggagcactatggtgaatgtagacaagagcaacttagcatgtgtggtcttgatggatccctctatgcaacctttgacaaggcagttgatgatcgaactgttggtcacaatcccgaacggttaagcttattgaaaggtggaattgtctacagcaatgcagtagtcacagtttctccaacatatctcaaagagacactctgctctggatggctttcaagtactttaatgagaaatcgtgataagtactttggtatagtaaatggaatagacactacgatgtggaacccttctactgatgttttcttgccttcgaaattcaatgttaaaaactctgagggcaagaagttatgcaaatactatgttcaaaggggacttggtttggcctcaggcgaccacgtgacagatactactctcaaggtgcctttggttgtctgtattactcgcttagttgcacaaaagggtcttcatttgattactcatgcaatcaagcaagttgaagaactaggtggacagatggttgtactaggaaagtccccggatcaccggattgaaagagaatttgaagaccttgcagattcgcttaaccaaggtcctagcattcggattttattgatgtatagtgaggagctatcccacttactttatgctgctgcagacattgttctagttccttccatgtatgagccatgtggactggcccaaatgattggcatgcgttatggggcagtcccagtagttagaaagactggtggtcttgcagatacagtctttgacatggatgatgagtcaaaccatgagatggcaaatgggtttgtttttgaaggaatggatgaagcttccctgaattgtgctctagatcgtgcatttgcttgctatagaaacaatccaggagaatggaattgcattgttaagaaggtaatgaagatggataatagctggaacaacacagctcgcaagtacattgagatttatgaatcagttacagcatcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
Glyco_transf_5 Glycos_transf_1

Protein Analysis

614

Amino Acids

68.26

Weight (kDa)

5.82

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_5 PF08323 121 - 349 1.4e-54 Starch synthase catalytic domain
Glyco_transf_4 PF13439 135 - 296 7e-08 Glycosyltransferase Family 4
Glycos_transf_1 PF00534 423 - 574 3.4e-13 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 425 - 567 2.8e-10 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 712
AatII GACGTC 1 cut(s) 649
Acc36I ACCTGC 1 cut(s) 712
AccB1I GGYRCC 2 cut(s) 388, 1270
AccB7I CCANNNNNTGG 2 cut(s) 853, 1651
AccI GTMKAC 2 cut(s) 874, 999
AccII CGCG 1 cut(s) 193
AciI CCGC 5 cut(s) 21, 113, 135, 143, 167
AclWI GGATC 5 cut(s) 559, 572, 903, 916, 1398
AcoI YGGCCR 1 cut(s) 457
AcsI RAATTY 3 cut(s) 223, 1166, 1409
AcvI CACGTG 2 cut(s) 455, 1249
AcyI GRCGYC 1 cut(s) 646
AfaI GTAC 5 cut(s) 5, 1072, 1097, 1374, 1811
AfiI CCNNNNNNNGG 3 cut(s) 307, 853, 1651
AflII CTTAAG 1 cut(s) 818
AflIII ACRYGT 1 cut(s) 798
AhdI GACNNNNNGTC 1 cut(s) 1602
AjnI CCWGG 1 cut(s) 1738
AleI CACNNNNGTG 1 cut(s) 864
AluBI AGCT 8 cut(s) 10, 380, 676, 978, 1480, 1688, 1788, 1802
AluI AGCT 8 cut(s) 10, 380, 676, 978, 1480, 1688, 1788, 1802
Alw21I GWGCWC 2 cut(s) 861, 1705
Alw26I GTCTC 1 cut(s) 1037
AlwI GGATC 5 cut(s) 559, 572, 903, 916, 1398
AlwNI CAGNNNCTG 1 cut(s) 31
Ama87I CYCGRG 2 cut(s) 65, 438
AoxI GGCC 5 cut(s) 178, 257, 457, 1233, 1548
ApeKI GCWGC 4 cut(s) 89, 92, 1499, 1502
ApoI RAATTY 3 cut(s) 223, 1166, 1409
ArsI GACNNNNNNTTYG 2 cut(s) 940, 972
Asp700I GAANNNNTTC 1 cut(s) 296
AspLEI GCGC 2 cut(s) 193, 195
AspS9I GGNCC 2 cut(s) 1442, 1549
AsuC2I CCSGG 1 cut(s) 1388
AsuHPI GGTGA 4 cut(s) 428, 736, 878, 1385
AsuII TTCGAA 1 cut(s) 1163
AvaI CYCGRG 2 cut(s) 65, 438
AvaII GGWCC 1 cut(s) 1442
AxyI CCTNAGG 1 cut(s) 1237
BalI TGGCCA 1 cut(s) 459
BamHI GGATCC 2 cut(s) 564, 908
BanI GGYRCC 2 cut(s) 388, 1270
BbrPI CACGTG 2 cut(s) 455, 1249
BbsI GAAGAC 2 cut(s) 1307, 1422
Bbv12I GWGCWC 2 cut(s) 861, 1705
BbvI GCAGC 4 cut(s) 101, 104, 1486, 1489
BccI CCATC 7 cut(s) 548, 631, 899, 1053, 1360, 1645, 1771
BcgI CGANNNNNNTGC 2 cut(s) 184, 218
BciT130I CCWGG 1 cut(s) 1740
BciVI GTATCC 1 cut(s) 506
BcnI CCSGG 1 cut(s) 1388
BcoDI GTCTC 1 cut(s) 1037
BfaI CTAG 5 cut(s) 1355, 1376, 1446, 1517, 1706
BfmI CTRYAG 3 cut(s) 1000, 1503, 1726
BfrI CTTAAG 1 cut(s) 818
BfuAI ACCTGC 1 cut(s) 712
BfuI GTATCC 1 cut(s) 506
BisI GCNGC 4 cut(s) 90, 93, 1500, 1503
BlsI GCNGC 4 cut(s) 91, 94, 1501, 1504
BmcAI AGTACT 2 cut(s) 1072, 1097
Bme1390I CCNGG 2 cut(s) 1388, 1740
Bme18I GGWCC 1 cut(s) 1442
BmeRI GACNNNNNGTC 1 cut(s) 1602
BmeT110I CYCGRG 2 cut(s) 65, 438
BmgT120I GGNCC 2 cut(s) 1442, 1549
BmiI GGNNCC 5 cut(s) 390, 566, 910, 1136, 1272
BmrFI CCNGG 2 cut(s) 1388, 1740
BmrI ACTGGG 2 cut(s) 599, 1577
BmsI GCATC 4 cut(s) 90, 178, 301, 708
BmuI ACTGGG 2 cut(s) 599, 1577
BpiI GAAGAC 2 cut(s) 1307, 1422
BpmI CTGGAG 1 cut(s) 698
Bpu14I TTCGAA 1 cut(s) 1163
BpuEI CTTGAG 1 cut(s) 1250
BpuMI CCSGG 1 cut(s) 1388
BsaAI YACGTR 2 cut(s) 455, 1249
BsaHI GRCGYC 1 cut(s) 646
BsaJI CCNNGG 4 cut(s) 198, 342, 1386, 1438
BsaWI WCCGGW 1 cut(s) 1395
BsaXI ACNNNNNCTCC 4 cut(s) 28, 58, 1009, 1039
Bsc4I CCNNNNNNNGG 3 cut(s) 307, 853, 1651
Bse1I ACTGG 8 cut(s) 71, 433, 566, 605, 733, 1551, 1583, 1603
Bse21I CCTNAGG 1 cut(s) 1237
Bse3DI GCAATG 2 cut(s) 207, 1012
BseBI CCWGG 1 cut(s) 1740
BseDI CCNNGG 4 cut(s) 198, 342, 1386, 1438
BseGI GGATG 8 cut(s) 81, 100, 316, 623, 723, 1064, 1636, 1687
BseLI CCNNNNNNNGG 3 cut(s) 307, 853, 1651
BseMI GCAATG 2 cut(s) 207, 1012
BseMII CTCAG 2 cut(s) 1176, 1251
BseNI ACTGG 8 cut(s) 71, 433, 566, 605, 733, 1551, 1583, 1603
BsePI GCGCGC 1 cut(s) 191
BseRI GAGGAG 1 cut(s) 1490
BseXI GCAGC 4 cut(s) 101, 104, 1486, 1489
BseYI CCCAGC 1 cut(s) 85
Bsh1236I CGCG 1 cut(s) 193
Bsh1285I CGRYCG 1 cut(s) 21
BshFI GGCC 5 cut(s) 180, 259, 459, 1235, 1550
BshNI GGYRCC 2 cut(s) 388, 1270
BsiEI CGRYCG 1 cut(s) 21
BsiHKAI GWGCWC 2 cut(s) 861, 1705
BsiHKCI CYCGRG 2 cut(s) 65, 438
BsiSI CCGG 3 cut(s) 47, 1388, 1396
BslFI GGGAC 5 cut(s) 657, 809, 1236, 1369, 1565
BslI CCNNNNNNNGG 3 cut(s) 307, 853, 1651
BsmAI GTCTC 1 cut(s) 1037
BsmFI GGGAC 5 cut(s) 657, 809, 1236, 1369, 1565
BsmI GAATGC 1 cut(s) 1449
BsnI GGCC 5 cut(s) 180, 259, 459, 1235, 1550
BsoBI CYCGRG 2 cut(s) 65, 438
Bsp119I TTCGAA 1 cut(s) 1163
Bsp1286I GDGCHC 2 cut(s) 861, 1705
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 5 cut(s) 564, 908, 943, 1390, 1708
BspACI CCGC 5 cut(s) 21, 113, 135, 143, 167
BspANI GGCC 5 cut(s) 180, 259, 459, 1235, 1550
BspCNI CTCAG 2 cut(s) 1177, 1250
BspFNI CGCG 1 cut(s) 193
BspHI TCATGA 2 cut(s) 550, 1841
BspLI GGNNCC 5 cut(s) 390, 566, 910, 1136, 1272
BspMAI CTGCAG 1 cut(s) 1507
BspMI ACCTGC 1 cut(s) 712
BspPI GGATC 5 cut(s) 559, 572, 903, 916, 1398
BspT104I TTCGAA 1 cut(s) 1163
BspT107I GGYRCC 2 cut(s) 388, 1270
BspTI CTTAAG 1 cut(s) 818
BsrDI GCAATG 2 cut(s) 207, 1012
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 8 cut(s) 71, 433, 566, 605, 733, 1551, 1583, 1603
BssECI CCNNGG 4 cut(s) 198, 342, 1386, 1438
BssHII GCGCGC 1 cut(s) 191
BssMI GATC 5 cut(s) 564, 908, 943, 1390, 1708
BssNI GRCGYC 1 cut(s) 646
BssT1I CCWWGG 2 cut(s) 342, 1438
Bst2UI CCWGG 1 cut(s) 1740
Bst4CI ACNGT 5 cut(s) 466, 952, 972, 1021, 1618
BstACI GRCGYC 1 cut(s) 646
BstAFI CTTAAG 1 cut(s) 818
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 2 cut(s) 455, 1249
BstBI TTCGAA 1 cut(s) 1163
BstC8I GCNNGC 7 cut(s) 117, 193, 706, 768, 1565, 1723, 1804
BstDEI CTNAG 4 cut(s) 887, 1185, 1237, 1297
BstF5I GGATG 8 cut(s) 81, 100, 316, 623, 723, 1064, 1636, 1687
BstFNI CGCG 1 cut(s) 193
BstHHI GCGC 2 cut(s) 193, 195
BstKTI GATC 5 cut(s) 567, 911, 946, 1393, 1711
BstMAI GTCTC 1 cut(s) 1037
BstMBI GATC 5 cut(s) 564, 908, 943, 1390, 1708
BstMCI CGRYCG 1 cut(s) 21
BstMWI GCNNNNNNNGC 2 cut(s) 386, 395
BstNI CCWGG 1 cut(s) 1740
BstNSI RCATGY 4 cut(s) 447, 802, 895, 1567
BstSCI CCNGG 2 cut(s) 1386, 1738
BstSFI CTRYAG 3 cut(s) 1000, 1503, 1726
BstUI CGCG 1 cut(s) 193
BstV1I GCAGC 4 cut(s) 101, 104, 1486, 1489
BstV2I GAAGAC 2 cut(s) 1307, 1422
BstX2I RGATCY 2 cut(s) 564, 908
BstXI CCANNNNNNTGG 2 cut(s) 1559, 1746
BstYI RGATCY 2 cut(s) 564, 908
Bsu36I CCTNAGG 1 cut(s) 1237
BsuI GTATCC 1 cut(s) 506
BsuRI GGCC 5 cut(s) 180, 259, 459, 1235, 1550
BtsCI GGATG 8 cut(s) 81, 100, 316, 623, 723, 1064, 1636, 1687
BveI ACCTGC 1 cut(s) 712
Cac8I GCNNGC 7 cut(s) 117, 193, 706, 768, 1565, 1723, 1804
CaiI CAGNNNCTG 1 cut(s) 31
CciI TCATGA 2 cut(s) 550, 1841
CfoI GCGC 2 cut(s) 193, 195
Cfr13I GGNCC 2 cut(s) 1442, 1549
CseI GACGC 1 cut(s) 37
Csp6I GTAC 5 cut(s) 4, 1071, 1096, 1373, 1810
CviQI GTAC 5 cut(s) 4, 1071, 1096, 1373, 1810
DdeI CTNAG 4 cut(s) 887, 1185, 1237, 1297
DpnI GATC 5 cut(s) 566, 910, 945, 1392, 1710
DpnII GATC 5 cut(s) 564, 908, 943, 1390, 1708
DriI GACNNNNNGTC 1 cut(s) 1602
EaeI YGGCCR 1 cut(s) 457
Eam1105I GACNNNNNGTC 1 cut(s) 1602
Eco130I CCWWGG 2 cut(s) 342, 1438
Eco32I GATATC 1 cut(s) 745
Eco47I GGWCC 1 cut(s) 1442
Eco72I CACGTG 2 cut(s) 455, 1249
Eco81I CCTNAGG 1 cut(s) 1237
Eco88I CYCGRG 2 cut(s) 65, 438
EcoO109I RGGNCCY 1 cut(s) 1442
EcoRII CCWGG 1 cut(s) 1738
EcoRV GATATC 1 cut(s) 745
EcoT14I CCWWGG 2 cut(s) 342, 1438
EcoT22I ATGCAT 1 cut(s) 580
ErhI CCWWGG 2 cut(s) 342, 1438
FalI AAGNNNNNCTT 2 cut(s) 870, 902
FaqI GGGAC 5 cut(s) 657, 809, 1236, 1369, 1565
FauI CCCGC 2 cut(s) 136, 160
FblI GTMKAC 2 cut(s) 874, 999
Fnu4HI GCNGC 4 cut(s) 90, 93, 1500, 1503
FokI GGATG 8 cut(s) 68, 87, 323, 610, 730, 1071, 1643, 1694
Fsp4HI GCNGC 4 cut(s) 90, 93, 1500, 1503
FspBI CTAG 5 cut(s) 1355, 1376, 1446, 1517, 1706
GlaI GCGC 2 cut(s) 192, 194
GluI GCNGC 4 cut(s) 90, 93, 1500, 1503
GsaI CCCAGC 1 cut(s) 89
GsuI CTGGAG 1 cut(s) 698
HaeIII GGCC 5 cut(s) 180, 259, 459, 1235, 1550
HapII CCGG 3 cut(s) 47, 1388, 1396
HgaI GACGC 1 cut(s) 37
HhaI GCGC 2 cut(s) 193, 195
Hin1I GRCGYC 1 cut(s) 646
Hin6I GCGC 2 cut(s) 191, 193
HinP1I GCGC 2 cut(s) 191, 193
HindIII AAGCTT 3 cut(s) 8, 976, 1686
HinfI GANTC 8 cut(s) 37, 97, 233, 347, 600, 1427, 1637, 1826
HpaII CCGG 3 cut(s) 47, 1388, 1396
HphI GGTGA 4 cut(s) 428, 736, 878, 1385
Hpy166II GTNNAC 4 cut(s) 875, 1000, 1361, 1544
Hpy188I TCNGA 4 cut(s) 238, 334, 1186, 1455
Hpy8I GTNNAC 4 cut(s) 875, 1000, 1361, 1544
Hpy99I CGWCG 1 cut(s) 111
HpyAV CCTTC 9 cut(s) 119, 270, 286, 312, 1149, 1170, 1533, 1667, 1759
HpyCH4III ACNGT 5 cut(s) 466, 952, 972, 1021, 1618
HpyCH4IV ACGT 4 cut(s) 454, 524, 646, 1248
HpyF10VI GCNNNNNNNGC 2 cut(s) 386, 395
HpyF3I CTNAG 4 cut(s) 887, 1185, 1237, 1297
HpySE526I ACGT 4 cut(s) 454, 524, 646, 1248
Hsp92I GRCGYC 1 cut(s) 646
HspAI GCGC 2 cut(s) 191, 193
Kzo9I GATC 5 cut(s) 564, 908, 943, 1390, 1708
LmnI GCTCC 2 cut(s) 856, 1477
Lsp1109I GCAGC 4 cut(s) 101, 104, 1486, 1489
LweI GCATC 4 cut(s) 90, 178, 301, 708
MaeI CTAG 5 cut(s) 1355, 1376, 1446, 1517, 1706
MaeII ACGT 4 cut(s) 454, 524, 646, 1248
MaeIII GTNAC 7 cut(s) 373, 424, 724, 956, 1015, 1249, 1831
MalI GATC 5 cut(s) 566, 910, 945, 1392, 1710
MboI GATC 5 cut(s) 564, 908, 943, 1390, 1708
MboII GAAGA 5 cut(s) 301, 1307, 1361, 1427, 1786
MfeI CAATTG 1 cut(s) 174
MflI RGATCY 2 cut(s) 564, 908
MhlI GDGCHC 2 cut(s) 861, 1705
MlsI TGGCCA 1 cut(s) 459
MluCI AATT 7 cut(s) 174, 223, 993, 1166, 1409, 1696, 1750
MluNI TGGCCA 1 cut(s) 459
MlyI GAGTC 2 cut(s) 31, 1646
MmeI TCCRAC 2 cut(s) 290, 1052
Mox20I TGGCCA 1 cut(s) 459
Mph1103I ATGCAT 1 cut(s) 580
MroXI GAANNNNTTC 1 cut(s) 296
MscI TGGCCA 1 cut(s) 459
MseI TTAA 8 cut(s) 527, 639, 819, 974, 1076, 1176, 1434, 1761
MslI CAYNNNNRTG 3 cut(s) 756, 864, 1127
Msp20I TGGCCA 1 cut(s) 459
MspA1I CMGCKG 1 cut(s) 380
MspCI CTTAAG 1 cut(s) 818
MspI CCGG 3 cut(s) 47, 1388, 1396
MspR9I CCNGG 2 cut(s) 1388, 1740
MunI CAATTG 1 cut(s) 174
Mva1269I GAATGC 1 cut(s) 1449
MvaI CCWGG 1 cut(s) 1740
MvnI CGCG 1 cut(s) 193
MwoI GCNNNNNNNGC 2 cut(s) 386, 395
NciI CCSGG 1 cut(s) 1388
NdeII GATC 5 cut(s) 564, 908, 943, 1390, 1708
NlaIV GGNNCC 5 cut(s) 390, 566, 910, 1136, 1272
NmeAIII GCCGAG 1 cut(s) 852
NmuCI GTSAC 4 cut(s) 724, 956, 1015, 1249
NsiI ATGCAT 1 cut(s) 580
NspI RCATGY 4 cut(s) 447, 802, 895, 1567
NspV TTCGAA 1 cut(s) 1163
OliI CACNNNNGTG 1 cut(s) 864
PaeI GCATGC 1 cut(s) 1567
PaeR7I CTCGAG 1 cut(s) 438
PagI TCATGA 2 cut(s) 550, 1841
PaqCI CACCTGC 1 cut(s) 712
PauI GCGCGC 1 cut(s) 191
PciI ACATGT 1 cut(s) 798
PctI GAATGC 1 cut(s) 1449
PdmI GAANNNNTTC 1 cut(s) 296
PfeI GAWTC 6 cut(s) 97, 233, 347, 600, 1427, 1826
PflMI CCANNNNNTGG 2 cut(s) 853, 1651
PfoI TCCNGGA 1 cut(s) 1738
PkrI GCNGC 4 cut(s) 91, 94, 1501, 1504
PleI GAGTC 2 cut(s) 31, 1645
PmaCI CACGTG 2 cut(s) 455, 1249
PmlI CACGTG 2 cut(s) 455, 1249
PpsI GAGTC 2 cut(s) 31, 1645
Ppu21I YACGTR 2 cut(s) 455, 1249
PpuMI RGGWCCY 1 cut(s) 1442
PscI ACATGT 1 cut(s) 798
Psp5II RGGWCCY 1 cut(s) 1442
Psp6I CCWGG 1 cut(s) 1738
PspCI CACGTG 2 cut(s) 455, 1249
PspFI CCCAGC 1 cut(s) 85
PspGI CCWGG 1 cut(s) 1738
PspN4I GGNNCC 5 cut(s) 390, 566, 910, 1136, 1272
PspPI GGNCC 2 cut(s) 1442, 1549
PspPPI RGGWCCY 1 cut(s) 1442
PspXI VCTCGAGB 1 cut(s) 438
PstI CTGCAG 1 cut(s) 1507
PstNI CAGNNNCTG 1 cut(s) 31
PsuI RGATCY 2 cut(s) 564, 908
PteI GCGCGC 1 cut(s) 191
PvuII CAGCTG 1 cut(s) 380
RsaI GTAC 5 cut(s) 5, 1072, 1097, 1374, 1811
RsaNI GTAC 5 cut(s) 4, 1071, 1096, 1373, 1810
RseI CAYNNNNRTG 3 cut(s) 756, 864, 1127
SaqAI TTAA 8 cut(s) 527, 639, 819, 974, 1076, 1176, 1434, 1761
SatI GCNGC 4 cut(s) 90, 93, 1500, 1503
Sau3AI GATC 5 cut(s) 564, 908, 943, 1390, 1708
Sau96I GGNCC 2 cut(s) 1442, 1549
ScaI AGTACT 2 cut(s) 1072, 1097
SchI GAGTC 2 cut(s) 31, 1646
ScrFI CCNGG 2 cut(s) 1388, 1740
SduI GDGCHC 2 cut(s) 861, 1705
SfaNI GCATC 4 cut(s) 90, 178, 301, 708
SfcI CTRYAG 3 cut(s) 1000, 1503, 1726
Sfr274I CTCGAG 1 cut(s) 438
SfuI TTCGAA 1 cut(s) 1163
SinI GGWCC 1 cut(s) 1442
SlaI CTCGAG 1 cut(s) 438
SmiMI CAYNNNNRTG 3 cut(s) 756, 864, 1127
SmlI CTYRAG 3 cut(s) 438, 818, 1265
SmoI CTYRAG 3 cut(s) 438, 818, 1265
SphI GCATGC 1 cut(s) 1567
Sse9I AATT 7 cut(s) 174, 223, 993, 1166, 1409, 1696, 1750
SsiI CCGC 5 cut(s) 21, 113, 135, 143, 167
SspMI CTAG 5 cut(s) 1355, 1376, 1446, 1517, 1706
StyD4I CCNGG 2 cut(s) 1386, 1738
StyI CCWWGG 2 cut(s) 342, 1438
TaaI ACNGT 5 cut(s) 466, 952, 972, 1021, 1618
TaiI ACGT 4 cut(s) 457, 527, 649, 1251
TaqI TCGA 6 cut(s) 17, 75, 155, 439, 946, 1163
TasI AATT 7 cut(s) 174, 223, 993, 1166, 1409, 1696, 1750
TatI WGTACW 5 cut(s) 3, 1070, 1095, 1372, 1809
TfiI GAWTC 6 cut(s) 97, 233, 347, 600, 1427, 1826
Tru1I TTAA 8 cut(s) 527, 639, 819, 974, 1076, 1176, 1434, 1761
Tru9I TTAA 8 cut(s) 527, 639, 819, 974, 1076, 1176, 1434, 1761
TseFI GTSAC 4 cut(s) 724, 956, 1015, 1249
TseI GCWGC 4 cut(s) 89, 92, 1499, 1502
Tsp45I GTSAC 4 cut(s) 724, 956, 1015, 1249
TspDTI ATGAA 8 cut(s) 288, 359, 654, 740, 1307, 1698, 1787, 1839
Van91I CCANNNNNTGG 2 cut(s) 853, 1651
Vha464I CTTAAG 1 cut(s) 818
VpaK11BI GGWCC 1 cut(s) 1442
XapI RAATTY 3 cut(s) 223, 1166, 1409
XbaI TCTAGA 1 cut(s) 1705
XceI RCATGY 4 cut(s) 447, 802, 895, 1567
XhoI CTCGAG 1 cut(s) 438
XmiI GTMKAC 2 cut(s) 874, 999
XmnI GAANNNNTTC 1 cut(s) 296
XspI CTAG 5 cut(s) 1355, 1376, 1446, 1517, 1706
ZraI GACGTC 1 cut(s) 647
ZrmI AGTACT 2 cut(s) 1072, 1097
Zsp2I ATGCAT 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.