Rmu_sc0003894.1_g000001

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003894.1
OG0014580
Physical Location & Seq
Forward (+)
6186 .. 15381
9196 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1440 bp
atggaggttgagacggagaggacgaaacccgttgctgtattcatggctttcggtaccaaaggcgatgtctatccaattgctgccattgcggcggcctttgcttccgaccagagacagtaccaagttgttcttatcacgcattcagctcacgagagcttacgttttcacttggctgagaagcatgtttcctatcttccaatttcatcaccacctgttctctctatccatcacaaacatgatactacaggctctgtagagttgcctctttcactgcagaaaaagttgttttctggagaacatagaaaagaatgtatttcggtagttgaaacgatatttggatcaggcccaagcatggatggtgatttcattgtgataaatttctttgctctggaaggctggagccttgcggagcttttccgtgtccgctgcatggttgctgctccttatgttgttccatacagtgcaccttcatcatttgagcgccagtttaaaaaggaatatcaacatttgtatagatacctgcaggaacctaacctcaggaaggttggttggaaagacgtgatccactggatgtggcctcttttcactgaacactggggatcctggagatgtgaccatttgaatctgagtgctttcccttttacagatccagtaacaggtctccccacttggcatgacaggccactgtctccccgacttttctcatgttcacttgcaatattttctgaagactcctacgcctatgccacctgtttttattggcctgaattctattgcatgggatttttgaagaatcctcaagcatttcttcgtatccttgaatctgtcctcaagataacaacttatcgttttatcctttttactgctggctacaagcctttagatgcagcaatccaagctattgctgctgattcatcatcgcacatgacccacaaacagattaatgacgattgcgtttctctcttccacgaacgattgttctgcttttctggttctataccatacacgtggctgttttctggatgtgcggctgcaattcatcatgggggaagtggaactactgctgcagcactacttgcaggaatcccacaggtactatgtccatttatgctagaccagttttattgggcagaaagaatgttttggcttggagtggcaccagaaccgttgaaaaggagccacttgcttcctgaagaaagtgatgacgaaagtatccaagaagcagccaagatgctatcaagagtcatacatgatacattatctcctgaaattagagaacgcgctgtagaaatttctgagagaatatctcttgaggacggagtctcaaatgctgttaggtatatcaaggaagagattgaactagcctccggcggagctggagaagccaccctgaaccagctccatgccatccaacatcccatcaagatggaagcaccatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

479

Amino Acids

54.19

Weight (kDa)

5.92

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EryCIII-like_C PF06722 332 - 387 2.5e-12 Erythromycin biosynthesis protein CIII-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014580)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24750
fragaria_vesca FvH4_5g30191 FvH4_5g30191
malus_domestica MD08G1224800.v1.1
prunus_persica Prupe.1G562100_v2.0.a1
pyrus_communis pycom08g19590
rosa_chinensis RchiOBHm_Chr7g0224451
rosa_laevigata RLG00000001894
rosa_multiflora Rmu_sc0003894.1_g000001
rosa_roxburghii Rroxscaffold_3G00234750
rosa_rugosa Rorug07G0223300 Rorug07G0223400
rosa_samantha Rh7AG379900 Rh7BG359500 Rh7CG399400 Rh7DG369100
rosa_wichuraiana Rw7G031180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 528
Acc65I GGTACC 1 cut(s) 53
AccB1I GGYRCC 2 cut(s) 53, 1156
AccII CGCG 1 cut(s) 1281
AciI CCGC 6 cut(s) 89, 92, 407, 424, 1028, 1371
AclWI GGATC 5 cut(s) 346, 556, 594, 607, 641
AcsI RAATTY 3 cut(s) 376, 767, 1290
AcuI CTGAAG 2 cut(s) 747, 1212
AcvI CACGTG 1 cut(s) 1008
AfaI GTAC 3 cut(s) 55, 119, 1095
AfiI CCNNNNNNNGG 4 cut(s) 352, 430, 541, 656
AflIII ACRYGT 1 cut(s) 1005
AgsI TTSAA 6 cut(s) 326, 622, 790, 821, 1171, 1358
AjiI CACGTC 1 cut(s) 559
AjnI CCWGG 1 cut(s) 602
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AluBI AGCT 6 cut(s) 146, 156, 412, 899, 1376, 1399
AluI AGCT 6 cut(s) 146, 156, 412, 899, 1376, 1399
Alw21I GWGCWC 1 cut(s) 466
Alw26I GTCTC 5 cut(s) 5, 106, 665, 693, 1327
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 5 cut(s) 346, 556, 594, 607, 641
AlwNI CAGNNNCTG 1 cut(s) 251
AoxI GGCC 5 cut(s) 93, 343, 575, 680, 761
ApaLI GTGCAC 1 cut(s) 462
ApeKI GCWGC 9 cut(s) 80, 426, 437, 887, 905, 1031, 1064, 1067, 1223
ApoI RAATTY 3 cut(s) 376, 767, 1290
ArsI GACNNNNNNTTYG 2 cut(s) 51, 83
AseI ATTAAT 1 cut(s) 942
Asp718I GGTACC 1 cut(s) 53
AspLEI GCGC 2 cut(s) 483, 1283
AspS9I GGNCC 1 cut(s) 344
AsuHPI GGTGA 2 cut(s) 198, 371
AxyI CCTNAGG 1 cut(s) 536
BaeGI GKGCMC 1 cut(s) 466
BaeI ACNNNNGTAYC 2 cut(s) 1195, 1228
BamHI GGATCC 1 cut(s) 599
BanI GGYRCC 2 cut(s) 53, 1156
BarI GAAGNNNNNNTAC 2 cut(s) 1042, 1074
BauI CACGAG 1 cut(s) 149
BbrPI CACGTG 1 cut(s) 1008
BbsI GAAGAC 1 cut(s) 735
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 9 cut(s) 67, 413, 424, 892, 899, 1018, 1051, 1079, 1235
BccI CCATC 5 cut(s) 234, 350, 1415, 1420, 1427
BcgI CGANNNNNNTGC 2 cut(s) 963, 997
BciT130I CCWGG 1 cut(s) 604
BciVI GTATCC 2 cut(s) 824, 1223
BcoDI GTCTC 5 cut(s) 5, 106, 665, 693, 1327
BfaI CTAG 2 cut(s) 1112, 1361
BfmI CTRYAG 6 cut(s) 243, 252, 272, 521, 1065, 1284
BfoI RGCGCY 1 cut(s) 484
BfuAI ACCTGC 1 cut(s) 528
BfuI GTATCC 2 cut(s) 824, 1223
BglI GCCNNNNNGGC 1 cut(s) 89
Bme1390I CCNGG 1 cut(s) 604
BmgBI CACGTC 1 cut(s) 559
BmgT120I GGNCC 1 cut(s) 344
BmiI GGNNCC 6 cut(s) 55, 401, 528, 601, 1158, 1178
BmrFI CCNGG 1 cut(s) 604
BmrI ACTGGG 1 cut(s) 604
BmsI GCATC 2 cut(s) 874, 1221
BmuI ACTGGG 1 cut(s) 604
BpiI GAAGAC 1 cut(s) 735
BplI GAGNNNNNCTC 2 cut(s) 1291, 1323
BpmI CTGGAG 4 cut(s) 312, 418, 625, 1398
BpuEI CTTGAG 3 cut(s) 783, 815, 1331
BsaAI YACGTR 1 cut(s) 1008
BsaBI GATNNNNATC 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 665
BsaXI ACNNNNNCTCC 2 cut(s) 1246, 1276
Bsc4I CCNNNNNNNGG 4 cut(s) 352, 430, 541, 656
Bse1I ACTGG 5 cut(s) 484, 572, 599, 650, 1117
Bse21I CCTNAGG 1 cut(s) 536
Bse3DI GCAATG 1 cut(s) 84
Bse8I GATNNNNATC 1 cut(s) 69
BseBI CCWGG 1 cut(s) 604
BseGI GGATG 5 cut(s) 361, 576, 1028, 1407, 1414
BseJI GATNNNNATC 1 cut(s) 69
BseLI CCNNNNNNNGG 4 cut(s) 352, 430, 541, 656
BseMI GCAATG 1 cut(s) 84
BseMII CTCAG 4 cut(s) 165, 550, 617, 1287
BseNI ACTGG 5 cut(s) 484, 572, 599, 650, 1117
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 9 cut(s) 67, 413, 424, 892, 899, 1018, 1051, 1079, 1235
Bsh1236I CGCG 1 cut(s) 1281
BshFI GGCC 5 cut(s) 95, 345, 577, 682, 763
BshNI GGYRCC 2 cut(s) 53, 1156
BsiHKAI GWGCWC 1 cut(s) 466
BsiSI CCGG 1 cut(s) 1368
BslI CCNNNNNNNGG 4 cut(s) 352, 430, 541, 656
BsmAI GTCTC 5 cut(s) 5, 106, 665, 693, 1327
BsmBI CGTCTC 1 cut(s) 5
BsmI GAATGC 1 cut(s) 139
BsnI GGCC 5 cut(s) 95, 345, 577, 682, 763
Bso31I GGTCTC 1 cut(s) 665
Bsp1286I GDGCHC 1 cut(s) 466
Bsp143I GATC 4 cut(s) 338, 561, 599, 646
BspACI CCGC 6 cut(s) 89, 92, 407, 424, 1028, 1371
BspANI GGCC 5 cut(s) 95, 345, 577, 682, 763
BspCNI CTCAG 4 cut(s) 166, 549, 618, 1288
BspFNI CGCG 1 cut(s) 1281
BspLI GGNNCC 6 cut(s) 55, 401, 528, 601, 1158, 1178
BspMAI CTGCAG 3 cut(s) 276, 525, 1069
BspMI ACCTGC 1 cut(s) 528
BspPI GGATC 5 cut(s) 346, 556, 594, 607, 641
BspT107I GGYRCC 2 cut(s) 53, 1156
BspTNI GGTCTC 1 cut(s) 665
BsrDI GCAATG 1 cut(s) 84
BsrI ACTGG 5 cut(s) 484, 572, 599, 650, 1117
BssMI GATC 4 cut(s) 338, 561, 599, 646
BssSI CACGAG 1 cut(s) 149
Bst2BI CACGAG 1 cut(s) 149
Bst2UI CCWGG 1 cut(s) 604
Bst4CI ACNGT 4 cut(s) 117, 461, 687, 1167
Bst6I CTCTTC 2 cut(s) 968, 1344
BstAPI GCANNNNNTGC 1 cut(s) 1076
BstBAI YACGTR 1 cut(s) 1008
BstC8I GCNNGC 1 cut(s) 868
BstDEI CTNAG 4 cut(s) 174, 536, 626, 1296
BstENI CCTNNNNNAGG 1 cut(s) 539
BstF5I GGATG 5 cut(s) 361, 576, 1028, 1407, 1414
BstFNI CGCG 1 cut(s) 1281
BstH2I RGCGCY 1 cut(s) 484
BstHHI GCGC 2 cut(s) 483, 1283
BstKTI GATC 4 cut(s) 341, 564, 602, 649
BstMAI GTCTC 5 cut(s) 5, 106, 665, 693, 1327
BstMBI GATC 4 cut(s) 338, 561, 599, 646
BstMWI GCNNNNNNNGC 8 cut(s) 86, 89, 98, 679, 896, 905, 1076, 1382
BstNI CCWGG 1 cut(s) 604
BstNSI RCATGY 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 602
BstSFI CTRYAG 6 cut(s) 243, 252, 272, 521, 1065, 1284
BstSLI GKGCMC 1 cut(s) 466
BstUI CGCG 1 cut(s) 1281
BstV1I GCAGC 9 cut(s) 67, 413, 424, 892, 899, 1018, 1051, 1079, 1235
BstV2I GAAGAC 1 cut(s) 735
BstX2I RGATCY 2 cut(s) 599, 646
BstXI CCANNNNNNTGG 2 cut(s) 1008, 1426
BstYI RGATCY 2 cut(s) 599, 646
Bsu36I CCTNAGG 1 cut(s) 536
BsuI GTATCC 2 cut(s) 824, 1223
BsuRI GGCC 5 cut(s) 95, 345, 577, 682, 763
BtgZI GCGATG 2 cut(s) 78, 903
BtrI CACGTC 1 cut(s) 559
BtsCI GGATG 5 cut(s) 361, 576, 1028, 1407, 1414
BtsI GCAGTG 1 cut(s) 269
BtsIMutI CAGTG 6 cut(s) 269, 466, 565, 585, 592, 683
BveI ACCTGC 1 cut(s) 528
Cac8I GCNNGC 1 cut(s) 868
CaiI CAGNNNCTG 1 cut(s) 251
CfoI GCGC 2 cut(s) 483, 1283
Cfr13I GGNCC 1 cut(s) 344
Csp6I GTAC 3 cut(s) 54, 118, 1094
CviQI GTAC 3 cut(s) 54, 118, 1094
DdeI CTNAG 4 cut(s) 174, 536, 626, 1296
DpnI GATC 4 cut(s) 340, 563, 601, 648
DpnII GATC 4 cut(s) 338, 561, 599, 646
DraI TTTAAA 1 cut(s) 490
Eam1104I CTCTTC 2 cut(s) 968, 1344
EarI CTCTTC 2 cut(s) 968, 1344
EciI GGCGGA 1 cut(s) 1386
Eco31I GGTCTC 1 cut(s) 665
Eco57I CTGAAG 2 cut(s) 747, 1212
Eco72I CACGTG 1 cut(s) 1008
Eco81I CCTNAGG 1 cut(s) 536
EcoNI CCTNNNNNAGG 1 cut(s) 539
EcoRI GAATTC 1 cut(s) 767
EcoRII CCWGG 1 cut(s) 602
Esp3I CGTCTC 1 cut(s) 5
FalI AAGNNNNNCTT 4 cut(s) 114, 146, 792, 824
FokI GGATG 5 cut(s) 368, 583, 1035, 1394, 1401
FspBI CTAG 2 cut(s) 1112, 1361
GlaI GCGC 2 cut(s) 482, 1282
GsuI CTGGAG 4 cut(s) 312, 418, 625, 1398
HaeII RGCGCY 1 cut(s) 484
HaeIII GGCC 5 cut(s) 95, 345, 577, 682, 763
HapII CCGG 1 cut(s) 1368
HhaI GCGC 2 cut(s) 483, 1283
Hin6I GCGC 2 cut(s) 481, 1281
HinP1I GCGC 2 cut(s) 481, 1281
HinfI GANTC 8 cut(s) 622, 731, 793, 821, 911, 1083, 1242, 1320
HpaII CCGG 1 cut(s) 1368
HphI GGTGA 2 cut(s) 198, 371
Hpy166II GTNNAC 2 cut(s) 464, 710
Hpy188I TCNGA 4 cut(s) 106, 627, 727, 1297
Hpy8I GTNNAC 2 cut(s) 464, 710
HpyAV CCTTC 3 cut(s) 386, 477, 535
HpyCH4III ACNGT 4 cut(s) 117, 461, 687, 1167
HpyCH4IV ACGT 3 cut(s) 160, 558, 1007
HpyF10VI GCNNNNNNNGC 8 cut(s) 86, 89, 98, 679, 896, 905, 1076, 1382
HpyF3I CTNAG 4 cut(s) 174, 536, 626, 1296
HpySE526I ACGT 3 cut(s) 160, 558, 1007
HspAI GCGC 2 cut(s) 481, 1281
KpnI GGTACC 1 cut(s) 57
Kzo9I GATC 4 cut(s) 338, 561, 599, 646
LmnI GCTCC 6 cut(s) 399, 409, 445, 1176, 1373, 1404
Lsp1109I GCAGC 9 cut(s) 67, 413, 424, 892, 899, 1018, 1051, 1079, 1235
LweI GCATC 2 cut(s) 874, 1221
MaeI CTAG 2 cut(s) 1112, 1361
MaeII ACGT 3 cut(s) 160, 558, 1007
MaeIII GTNAC 2 cut(s) 611, 652
MalI GATC 4 cut(s) 340, 563, 601, 648
MboI GATC 4 cut(s) 338, 561, 599, 646
MboII GAAGA 7 cut(s) 185, 740, 800, 802, 955, 1205, 1361
MfeI CAATTG 1 cut(s) 75
MflI RGATCY 2 cut(s) 599, 646
MhlI GDGCHC 1 cut(s) 466
MluCI AATT 7 cut(s) 75, 198, 376, 767, 1035, 1269, 1290
MlyI GAGTC 3 cut(s) 725, 1251, 1329
MmeI TCCRAC 3 cut(s) 129, 530, 1435
MnlI CCTC 8 cut(s) 12, 273, 545, 588, 807, 839, 1306, 1375
MseI TTAA 2 cut(s) 489, 942
MslI CAYNNNNRTG 3 cut(s) 234, 1006, 1424
MspA1I CMGCKG 1 cut(s) 426
MspI CCGG 1 cut(s) 1368
MspR9I CCNGG 1 cut(s) 604
MunI CAATTG 1 cut(s) 75
Mva1269I GAATGC 1 cut(s) 139
MvaI CCWGG 1 cut(s) 604
MvnI CGCG 1 cut(s) 1281
MwoI GCNNNNNNNGC 8 cut(s) 86, 89, 98, 679, 896, 905, 1076, 1382
NdeII GATC 4 cut(s) 338, 561, 599, 646
NlaIV GGNNCC 6 cut(s) 55, 401, 528, 601, 1158, 1178
NmuCI GTSAC 1 cut(s) 611
NspI RCATGY 1 cut(s) 185
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PctI GAATGC 1 cut(s) 139
PfeI GAWTC 5 cut(s) 622, 793, 821, 911, 1083
PflFI GACNNNGTC 1 cut(s) 1319
PfoI TCCNGGA 1 cut(s) 602
PleI GAGTC 3 cut(s) 725, 1250, 1328
PmaCI CACGTG 1 cut(s) 1008
PmlI CACGTG 1 cut(s) 1008
PpsI GAGTC 3 cut(s) 725, 1250, 1328
Ppu21I YACGTR 1 cut(s) 1008
PshBI ATTAAT 1 cut(s) 942
Psp6I CCWGG 1 cut(s) 602
PspCI CACGTG 1 cut(s) 1008
PspGI CCWGG 1 cut(s) 602
PspN4I GGNNCC 6 cut(s) 55, 401, 528, 601, 1158, 1178
PspPI GGNCC 1 cut(s) 344
PstI CTGCAG 3 cut(s) 276, 525, 1069
PstNI CAGNNNCTG 1 cut(s) 251
PsuI RGATCY 2 cut(s) 599, 646
PsyI GACNNNGTC 1 cut(s) 1319
RsaI GTAC 3 cut(s) 55, 119, 1095
RsaNI GTAC 3 cut(s) 54, 118, 1094
RseI CAYNNNNRTG 3 cut(s) 234, 1006, 1424
SaqAI TTAA 2 cut(s) 489, 942
Sau3AI GATC 4 cut(s) 338, 561, 599, 646
Sau96I GGNCC 1 cut(s) 344
SbfI CCTGCAGG 1 cut(s) 525
SchI GAGTC 3 cut(s) 725, 1251, 1329
ScrFI CCNGG 1 cut(s) 604
SdaI CCTGCAGG 1 cut(s) 525
SduI GDGCHC 1 cut(s) 466
SfaNI GCATC 2 cut(s) 874, 1221
SfcI CTRYAG 6 cut(s) 243, 252, 272, 521, 1065, 1284
SmiMI CAYNNNNRTG 3 cut(s) 234, 1006, 1424
SmlI CTYRAG 3 cut(s) 798, 830, 1310
SmoI CTYRAG 3 cut(s) 798, 830, 1310
Sse8387I CCTGCAGG 1 cut(s) 525
Sse9I AATT 7 cut(s) 75, 198, 376, 767, 1035, 1269, 1290
SsiI CCGC 6 cut(s) 89, 92, 407, 424, 1028, 1371
SspI AATATT 1 cut(s) 720
SspMI CTAG 2 cut(s) 1112, 1361
StyD4I CCNGG 1 cut(s) 602
TaaI ACNGT 4 cut(s) 117, 461, 687, 1167
TaiI ACGT 3 cut(s) 163, 561, 1010
TasI AATT 7 cut(s) 75, 198, 376, 767, 1035, 1269, 1290
TauI GCSGC 3 cut(s) 92, 95, 1031
TfiI GAWTC 5 cut(s) 622, 793, 821, 911, 1083
Tru1I TTAA 2 cut(s) 489, 942
Tru9I TTAA 2 cut(s) 489, 942
TscAI CASTG 6 cut(s) 276, 466, 572, 592, 599, 690
TseFI GTSAC 1 cut(s) 611
TseI GCWGC 9 cut(s) 80, 426, 437, 887, 905, 1031, 1064, 1067, 1223
Tsp45I GTSAC 1 cut(s) 611
TspDTI ATGAA 6 cut(s) 31, 192, 355, 459, 903, 1028
TspGWI ACGGA 3 cut(s) 29, 407, 1332
TspRI CASTG 6 cut(s) 276, 466, 572, 592, 599, 690
Tth111I GACNNNGTC 1 cut(s) 1319
VneI GTGCAC 1 cut(s) 462
VspI ATTAAT 1 cut(s) 942
XagI CCTNNNNNAGG 1 cut(s) 539
XapI RAATTY 3 cut(s) 376, 767, 1290
XceI RCATGY 1 cut(s) 185
XspI CTAG 2 cut(s) 1112, 1361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.