Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0004283.1

Orthogroup ID

OG0015722

Physical Location & Seq

Genomic Coordinates Forward (+)

182062 .. 187812

Gene Structure 5751 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 876 bp

atgttaatgcttaaagtttggcggtggccgaaccttcgtttaacacgggtctggtgggcggaccactactctgaggacttgatgattttcgctagctatcacatgaaagatagggcgtcagagggagaccgcgttgggcggtcttctcttctccgatgcctaagtgaggagagagttgatcttctccttgttttcgatgtgggactgatgtgcttcaattcccagcgtctggagcttctgatgctatcttggcgcggcgcgtggcggcgcgtcagcagtgatctgggggtaacccggggctcaagcggtagcccgcctggctgtgtttccgtaggttactcctttggcgggagtcggtaccgctggcggcgcggggcgctgggtgggctgcgcaagcgtgggacgcgggctgtgttaaggagacttatgacagatgtaccatttggtgttcttttgtccggagggttggactcatcactcgttgctgctgtggcttgtcactatttggctaaatcagaagctgcaagtcagtgggggcctgatcttgggaggattgagaagtgggtcttacgcaaagcatttgatgatgatcagaagccatatctaccaaaggcttcgaagcagtttagtgatggtgtcggttacagctggattgacggcttgaaagatcacgccaacaaacaaaatgctgcaagattgacagtttttggtggtccgagtgtggcatgcagcacagcaaaagctgtggagtgggatgcggaatggtccaagaaccctgatccatctggtcgtgctgcacttggtatacatgcagctgcatatgaggaatccaaagatgtgaagactagaagtcctgtgagcaagtggctgcagtag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009642 GO:0009646 GO:0009651 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0030054 GO:0032787 GO:0034641 GO:0042538 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046983 GO:0050896 GO:0055044 GO:0071704 GO:0072330 GO:0097164 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00250 ko01100 ko01110 map00250 map01100 map01110

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

291

Amino Acids

32.49

Weight (kDa)

9.68

Isoelectric Point (pI)

51.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asn_synthase	PF00733	135 - 170	1.4e-10	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015722)

Species	Orthologous Gene IDs
pyrus_communis	pycom08g18330 pycom10g26420 pycom13g04680 pycom14g14050
rosa_laevigata	RLG00000024517 RLG00000030191
rosa_multiflora	Rmu_sc0003130.1_g000014 Rmu_sc0003706.1_g000002 Rmu_sc0004283.1_g000045 Rmu_sc0007734.1_g000014
rosa_roxburghii	Rroxscaffold_2G00083090 Rroxscaffold_6G00394300
rosa_rugosa	Rorug01G0156200.1 Rorug01G0407800
rosa_samantha	Rh4CG277800 Rh4DG262800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	392
Acc65I	GGTACC	1 cut(s)	357
AccB1I	GGYRCC	1 cut(s)	357
AccB7I	CCANNNNNTGG	1 cut(s)	229
AccI	GTMKAC	1 cut(s)	805
AccII	CGCG	6 cut(s)	132, 255, 260, 270, 372, 406
AccIII	TCCGGA	1 cut(s)	458
AclWI	GGATC	1 cut(s)	773
AcoI	YGGCCR	1 cut(s)	26
AcyI	GRCGYC	1 cut(s)	116
AfaI	GTAC	2 cut(s)	359, 438
AfiI	CCNNNNNNNGG	4 cut(s)	166, 229, 348, 545
AgsI	TTSAA	2 cut(s)	217, 664
AhdI	GACNNNNNGTC	1 cut(s)	849
AjnI	CCWGG	1 cut(s)	316
AluBI	AGCT	6 cut(s)	96, 235, 521, 648, 743, 815
AluI	AGCT	6 cut(s)	96, 235, 521, 648, 743, 815
Alw26I	GTCTC	2 cut(s)	120, 415
AlwI	GGATC	1 cut(s)	773
AlwNI	CAGNNNCTG	2 cut(s)	229, 521
Ama87I	CYCGRG	1 cut(s)	294
Aor13HI	TCCGGA	1 cut(s)	458
AoxI	GGCC	2 cut(s)	26, 536
ApeKI	GCWGC	9 cut(s)	388, 485, 521, 689, 729, 794, 812, 815, 868
Asp718I	GGTACC	1 cut(s)	357
AspLEI	GCGC	6 cut(s)	255, 260, 270, 372, 379, 393
AspS9I	GGNCC	4 cut(s)	61, 536, 713, 765
AsuC2I	CCSGG	2 cut(s)	295, 296
AsuII	TTCGAA	1 cut(s)	617
AsuNHI	GCTAGC	1 cut(s)	92
AvaI	CYCGRG	1 cut(s)	294
AvaII	GGWCC	3 cut(s)	61, 713, 765
BaeI	ACNNNNGTAYC	2 cut(s)	420, 453
BanI	GGYRCC	1 cut(s)	357
BanII	GRGCYC	1 cut(s)	302
BbsI	GAAGAC	2 cut(s)	135, 848
BbvI	GCAGC	9 cut(s)	375, 472, 508, 676, 741, 781, 802, 824, 855
BccI	CCATC	2 cut(s)	626, 790
BceAI	ACGGC	1 cut(s)	673
BciT130I	CCWGG	1 cut(s)	318
BclI	TGATCA	1 cut(s)	589
BcnI	CCSGG	2 cut(s)	295, 296
BcoDI	GTCTC	2 cut(s)	120, 415
BfaI	CTAG	2 cut(s)	93, 846
BfmI	CTRYAG	1 cut(s)	869
BfoI	RGCGCY	1 cut(s)	380
BglI	GCCNNNNNGGC	1 cut(s)	318
Bme1390I	CCNGG	3 cut(s)	295, 296, 318
Bme18I	GGWCC	3 cut(s)	61, 713, 765
BmeRI	GACNNNNNGTC	1 cut(s)	849
BmeT110I	CYCGRG	1 cut(s)	294
BmgT120I	GGNCC	4 cut(s)	61, 536, 713, 765
BmiI	GGNNCC	2 cut(s)	359, 537
BmrFI	CCNGG	3 cut(s)	295, 296, 318
BmsI	GCATC	3 cut(s)	146, 231, 745
BmtI	GCTAGC	1 cut(s)	96
BpiI	GAAGAC	2 cut(s)	135, 848
BpmI	CTGGAG	1 cut(s)	251
Bpu14I	TTCGAA	1 cut(s)	617
BpuEI	CTTGAG	1 cut(s)	286
BpuMI	CCSGG	2 cut(s)	295, 296
BsaBI	GATNNNNATC	1 cut(s)	588
BsaHI	GRCGYC	1 cut(s)	116
BsaI	GGTCTC	1 cut(s)	120
BsaJI	CCNNGG	2 cut(s)	294, 295
BsaWI	WCCGGW	1 cut(s)	458
BsaXI	ACNNNNNCTCC	2 cut(s)	117, 147
Bsc4I	CCNNNNNNNGG	4 cut(s)	166, 229, 348, 545
Bse8I	GATNNNNATC	1 cut(s)	588
BseAI	TCCGGA	1 cut(s)	458
BseBI	CCWGG	1 cut(s)	318
BseDI	CCNNGG	2 cut(s)	294, 295
BseGI	GGATG	1 cut(s)	760
BseJI	GATNNNNATC	1 cut(s)	588
BseLI	CCNNNNNNNGG	4 cut(s)	166, 229, 348, 545
BseMII	CTCAG	1 cut(s)	63
BseRI	GAGGAG	1 cut(s)	182
BseXI	GCAGC	9 cut(s)	375, 472, 508, 676, 741, 781, 802, 824, 855
BseYI	CCCAGC	2 cut(s)	222, 379
BsgI	GTGCAG	1 cut(s)	780
Bsh1236I	CGCG	6 cut(s)	132, 255, 260, 270, 372, 406
BshFI	GGCC	2 cut(s)	28, 538
BshNI	GGYRCC	1 cut(s)	357
BsiHKCI	CYCGRG	1 cut(s)	294
BsiSI	CCGG	2 cut(s)	295, 459
BslFI	GGGAC	2 cut(s)	216, 415
BslI	CCNNNNNNNGG	4 cut(s)	166, 229, 348, 545
BsmAI	GTCTC	2 cut(s)	120, 415
BsmFI	GGGAC	2 cut(s)	216, 415
BsnI	GGCC	2 cut(s)	28, 538
Bso31I	GGTCTC	1 cut(s)	120
BsoBI	CYCGRG	1 cut(s)	294
Bsp119I	TTCGAA	1 cut(s)	617
Bsp1286I	GDGCHC	1 cut(s)	302
Bsp13I	TCCGGA	1 cut(s)	458
Bsp143I	GATC	6 cut(s)	178, 280, 541, 589, 667, 778
BspANI	GGCC	2 cut(s)	28, 538
BspCNI	CTCAG	1 cut(s)	64
BspEI	TCCGGA	1 cut(s)	458
BspFNI	CGCG	6 cut(s)	132, 255, 260, 270, 372, 406
BspLI	GGNNCC	2 cut(s)	359, 537
BspMAI	CTGCAG	1 cut(s)	873
BspOI	GCTAGC	1 cut(s)	96
BspPI	GGATC	1 cut(s)	773
BspT104I	TTCGAA	1 cut(s)	617
BspT107I	GGYRCC	1 cut(s)	357
BspTNI	GGTCTC	1 cut(s)	120
BssECI	CCNNGG	2 cut(s)	294, 295
BssMI	GATC	6 cut(s)	178, 280, 541, 589, 667, 778
BssNAI	GTATAC	1 cut(s)	806
BssNI	GRCGYC	1 cut(s)	116
Bst1107I	GTATAC	1 cut(s)	806
Bst2UI	CCWGG	1 cut(s)	318
Bst4CI	ACNGT	1 cut(s)	703
Bst6I	CTCTTC	1 cut(s)	153
BstACI	GRCGYC	1 cut(s)	116
BstBI	TTCGAA	1 cut(s)	617
BstC8I	GCNNGC	6 cut(s)	94, 314, 365, 395, 408, 727
BstDEI	CTNAG	2 cut(s)	72, 161
BstEII	GGTNACC	1 cut(s)	289
BstENI	CCTNNNNNAGG	1 cut(s)	164
BstF5I	GGATG	1 cut(s)	760
BstFNI	CGCG	6 cut(s)	132, 255, 260, 270, 372, 406
BstH2I	RGCGCY	1 cut(s)	380
BstHHI	GCGC	6 cut(s)	255, 260, 270, 372, 379, 393
BstKTI	GATC	6 cut(s)	181, 283, 544, 592, 670, 781
BstMAI	GTCTC	2 cut(s)	120, 415
BstMBI	GATC	6 cut(s)	178, 280, 541, 589, 667, 778
BstNI	CCWGG	1 cut(s)	318
BstNSI	RCATGY	2 cut(s)	729, 812
BstPI	GGTNACC	1 cut(s)	289
BstSCI	CCNGG	3 cut(s)	293, 294, 316
BstSFI	CTRYAG	1 cut(s)	869
BstUI	CGCG	6 cut(s)	132, 255, 260, 270, 372, 406
BstV1I	GCAGC	9 cut(s)	375, 472, 508, 676, 741, 781, 802, 824, 855
BstV2I	GAAGAC	2 cut(s)	135, 848
BstZ17I	GTATAC	1 cut(s)	806
BsuRI	GGCC	2 cut(s)	28, 538
BtsCI	GGATG	1 cut(s)	760
BtsI	GCAGTG	1 cut(s)	283
BtsIMutI	CAGTG	2 cut(s)	283, 536
Cac8I	GCNNGC	6 cut(s)	94, 314, 365, 395, 408, 727
CaiI	CAGNNNCTG	2 cut(s)	229, 521
CfoI	GCGC	6 cut(s)	255, 260, 270, 372, 379, 393
Cfr13I	GGNCC	4 cut(s)	61, 536, 713, 765
Cfr9I	CCCGGG	1 cut(s)	294
CseI	GACGC	4 cut(s)	105, 215, 259, 412
Csp6I	GTAC	2 cut(s)	358, 437
CspCI	CAANNNNNGTGG	2 cut(s)	726, 761
CviAII	CATG	3 cut(s)	103, 726, 809
CviQI	GTAC	2 cut(s)	358, 437
DdeI	CTNAG	2 cut(s)	72, 161
DpnI	GATC	6 cut(s)	180, 282, 543, 591, 669, 780
DpnII	GATC	6 cut(s)	178, 280, 541, 589, 667, 778
DriI	GACNNNNNGTC	1 cut(s)	849
EaeI	YGGCCR	1 cut(s)	26
Eam1104I	CTCTTC	1 cut(s)	153
Eam1105I	GACNNNNNGTC	1 cut(s)	849
EarI	CTCTTC	1 cut(s)	153
EciI	GGCGGA	1 cut(s)	74
Eco24I	GRGCYC	1 cut(s)	302
Eco31I	GGTCTC	1 cut(s)	120
Eco47I	GGWCC	3 cut(s)	61, 713, 765
Eco88I	CYCGRG	1 cut(s)	294
Eco91I	GGTNACC	1 cut(s)	289
EcoNI	CCTNNNNNAGG	1 cut(s)	164
EcoO109I	RGGNCCY	1 cut(s)	536
EcoO65I	GGTNACC	1 cut(s)	289
EcoRII	CCWGG	1 cut(s)	316
EcoT38I	GRGCYC	1 cut(s)	302
FaeI	CATG	3 cut(s)	106, 729, 812
FaiI	YATR	8 cut(s)	104, 428, 601, 727, 806, 810, 820, 822
FalI	AAGNNNNNCTT	2 cut(s)	551, 583
FaqI	GGGAC	2 cut(s)	216, 415
FatI	CATG	3 cut(s)	102, 725, 808
FauI	CCCGC	4 cut(s)	321, 341, 365, 399
FauNDI	CATATG	1 cut(s)	820
FbaI	TGATCA	1 cut(s)	589
FblI	GTMKAC	1 cut(s)	805
FokI	GGATG	1 cut(s)	767
FriOI	GRGCYC	1 cut(s)	302
FspBI	CTAG	2 cut(s)	93, 846
FspI	TGCGCA	1 cut(s)	392
GlaI	GCGC	6 cut(s)	254, 259, 269, 371, 378, 392
GsaI	CCCAGC	2 cut(s)	226, 383
GsuI	CTGGAG	1 cut(s)	251
HaeII	RGCGCY	1 cut(s)	380
HaeIII	GGCC	2 cut(s)	28, 538
HapII	CCGG	2 cut(s)	295, 459
HgaI	GACGC	4 cut(s)	105, 215, 259, 412
HhaI	GCGC	6 cut(s)	255, 260, 270, 372, 379, 393
Hin1I	GRCGYC	1 cut(s)	116
Hin1II	CATG	3 cut(s)	106, 729, 812
Hin6I	GCGC	6 cut(s)	253, 258, 268, 370, 377, 391
HinP1I	GCGC	6 cut(s)	253, 258, 268, 370, 377, 391
HinfI	GANTC	3 cut(s)	352, 470, 827
HpaII	CCGG	2 cut(s)	295, 459
Hpy166II	GTNNAC	1 cut(s)	806
Hpy188I	TCNGA	7 cut(s)	73, 121, 155, 240, 517, 594, 717
Hpy188III	TCNNGA	2 cut(s)	230, 459
Hpy8I	GTNNAC	1 cut(s)	806
HpyAV	CCTTC	1 cut(s)	44
HpyCH4III	ACNGT	1 cut(s)	703
HpyCH4V	TGCA	7 cut(s)	524, 692, 729, 797, 812, 818, 871
HpyF3I	CTNAG	2 cut(s)	72, 161
Hsp92I	GRCGYC	1 cut(s)	116
Hsp92II	CATG	3 cut(s)	106, 729, 812
HspAI	GCGC	6 cut(s)	253, 258, 268, 370, 377, 391
Kpn2I	TCCGGA	1 cut(s)	458
KpnI	GGTACC	1 cut(s)	361
Ksp22I	TGATCA	1 cut(s)	589
Kzo9I	GATC	6 cut(s)	178, 280, 541, 589, 667, 778
LmnI	GCTCC	1 cut(s)	232
Lsp1109I	GCAGC	9 cut(s)	375, 472, 508, 676, 741, 781, 802, 824, 855
LweI	GCATC	3 cut(s)	146, 231, 745
MaeI	CTAG	2 cut(s)	93, 846
MaeIII	GTNAC	4 cut(s)	289, 335, 497, 641
MalI	GATC	6 cut(s)	180, 282, 543, 591, 669, 780
MboI	GATC	6 cut(s)	178, 280, 541, 589, 667, 778
MboII	GAAGA	4 cut(s)	135, 140, 173, 853
MhlI	GDGCHC	1 cut(s)	302
MluCI	AATT	1 cut(s)	217
MlyI	GAGTC	2 cut(s)	361, 464
MmeI	TCCRAC	1 cut(s)	447
MnlI	CCTC	6 cut(s)	67, 115, 160, 455, 543, 817
MroI	TCCGGA	1 cut(s)	458
MseI	TTAA	4 cut(s)	5, 12, 41, 416
MspA1I	CMGCKG	3 cut(s)	363, 648, 815
MspI	CCGG	2 cut(s)	295, 459
MspR9I	CCNGG	3 cut(s)	295, 296, 318
MteI	GCGCNGCGC	1 cut(s)	256
MvaI	CCWGG	1 cut(s)	318
MvnI	CGCG	6 cut(s)	132, 255, 260, 270, 372, 406
NciI	CCSGG	2 cut(s)	295, 296
NdeI	CATATG	1 cut(s)	820
NdeII	GATC	6 cut(s)	178, 280, 541, 589, 667, 778
NheI	GCTAGC	1 cut(s)	92
NlaIII	CATG	3 cut(s)	106, 729, 812
NlaIV	GGNNCC	2 cut(s)	359, 537
NmuCI	GTSAC	1 cut(s)	497
NsbI	TGCGCA	1 cut(s)	392
NspI	RCATGY	2 cut(s)	729, 812
NspV	TTCGAA	1 cut(s)	617
PaeI	GCATGC	1 cut(s)	729
PfeI	GAWTC	1 cut(s)	827
PflMI	CCANNNNNTGG	1 cut(s)	229
PleI	GAGTC	2 cut(s)	360, 464
PpsI	GAGTC	2 cut(s)	360, 464
Psp6I	CCWGG	1 cut(s)	316
PspEI	GGTNACC	1 cut(s)	289
PspFI	CCCAGC	2 cut(s)	222, 379
PspGI	CCWGG	1 cut(s)	316
PspN4I	GGNNCC	2 cut(s)	359, 537
PspPI	GGNCC	4 cut(s)	61, 536, 713, 765
PstI	CTGCAG	1 cut(s)	873
PstNI	CAGNNNCTG	2 cut(s)	229, 521
PvuII	CAGCTG	2 cut(s)	648, 815
RsaI	GTAC	2 cut(s)	359, 438
RsaNI	GTAC	2 cut(s)	358, 437
SaqAI	TTAA	4 cut(s)	5, 12, 41, 416
Sau3AI	GATC	6 cut(s)	178, 280, 541, 589, 667, 778
Sau96I	GGNCC	4 cut(s)	61, 536, 713, 765
SchI	GAGTC	2 cut(s)	361, 464
ScrFI	CCNGG	3 cut(s)	295, 296, 318
SduI	GDGCHC	1 cut(s)	302
SetI	ASST	8 cut(s)	36, 98, 237, 337, 523, 650, 745, 817
SfaNI	GCATC	3 cut(s)	146, 231, 745
SfcI	CTRYAG	1 cut(s)	869
SfuI	TTCGAA	1 cut(s)	617
SinI	GGWCC	3 cut(s)	61, 713, 765
SmaI	CCCGGG	1 cut(s)	296
SmlI	CTYRAG	1 cut(s)	301
SmoI	CTYRAG	1 cut(s)	301
SphI	GCATGC	1 cut(s)	729
Sse9I	AATT	1 cut(s)	217
SspMI	CTAG	2 cut(s)	93, 846
StyD4I	CCNGG	3 cut(s)	293, 294, 316
TaaI	ACNGT	1 cut(s)	703
TaqI	TCGA	2 cut(s)	195, 617
TasI	AATT	1 cut(s)	217
TauI	GCSGC	3 cut(s)	258, 268, 370
TfiI	GAWTC	1 cut(s)	827
Tru1I	TTAA	4 cut(s)	5, 12, 41, 416
Tru9I	TTAA	4 cut(s)	5, 12, 41, 416
TscAI	CASTG	2 cut(s)	283, 536
TseFI	GTSAC	1 cut(s)	497
TseI	GCWGC	9 cut(s)	388, 485, 521, 689, 729, 794, 812, 815, 868
Tsp45I	GTSAC	1 cut(s)	497
TspDTI	ATGAA	1 cut(s)	119
TspGWI	ACGGA	1 cut(s)	319
TspMI	CCCGGG	1 cut(s)	294
TspRI	CASTG	2 cut(s)	283, 536
Van91I	CCANNNNNTGG	1 cut(s)	229
VpaK11BI	GGWCC	3 cut(s)	61, 713, 765
XagI	CCTNNNNNAGG	1 cut(s)	164
XceI	RCATGY	2 cut(s)	729, 812
XmaI	CCCGGG	1 cut(s)	294
XmiI	GTMKAC	1 cut(s)	805
XspI	CTAG	2 cut(s)	93, 846

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.