Rmu_sc0004296.1_g000004

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004296.1
OG0005500
Physical Location & Seq
Forward (+)
9984 .. 24396
14413 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5187 bp
atggtaaagaaaaccgggttcaggtcactgaagctggtgaacatagagatggaccaagttctgggagacaagcccgtcggagttgactatggcaggcttgataatggtctatcttactatgttcgttgcaattctaagcccaaaatgagagcagctcttgcccttgctgttaaagtcgggtcagttttggaagaggaggaagaacgaggagttgcccacattgttgagcatcttgctttcagtgctactcagaagtcagttttggaagaggaggaagaacgaggagttgcccacattgttgagcatcttgctttcagtgctactcagaagtacacaaatcatgatatagtcagatttcttgaaagtattggggcagaatttggcgcttgccagaatgcagtgacatcggctgatgacactgtttatgagttatttgttcctgttgacaagcatgaactattatctgaggccatttctgtcttggcggaattcagttctgagattcgtgtctcaaaagacgatgtggaaagagaaagaggcgctgttatggaagagtacagagggaatcgaaacgctacgggaaggatgcaggatgcgcattggcttttaatgatggctggttcaaagtatgctgagcgtttacctattggattagaaaaggtaattcgaactgtttctcctgagactgtgaagcaattttatcagaagtggtaccatttaagcaatatggcagtgatagctgtaggagatttcccggatactgagagtgtagttgagttgataaagaatcaatttggggataaaagttctgcacctgagcgtccacttataccaacatatcaagttccatctcatgaagagccacgttattcttgtttcattgaatctgaggcaactgggtctgcagtgattattagctataaggctccagctgttgagctgaatacagtgagggactatagggatttactggcagaatcaatgttcctttatgctctaaaccagaggttctttaaaatagctcgtagaaaagatccaccctttttctcatgctcaagttccgcagatgttctagttaatcccttgaaggcttatatgatgacctcatcttgtaaagaaaagggaacaattcaggccctagagtcgatgctgattgaggttgccagggtacggctacatgggttttcagaacgggaagtgtctatagttcgagctttactgatgtcagagattgaatctgcatatttggaacgcgatcaaatgcagtcaactagcttgcgagatgaatatttacagcattttctccgtaatgaacctgttattgggattgagtatgaggctcaactccagaagacccttctaccttatttaacagcagcagagatatccaaatatgctgagaagttacaaacatcatgcagctgtgtcattaagacaattgaaccacgagcttctgcaatagtagaagatttgaaaaatgttgtctctaaaatcagtgctcttgaggaagaaagaatcatttctccatgggatgaagaacatattccagaagaaattgttagcacaaagcctaatccagggaatattgtgcagcaatgtgaatattcaaatattggagctactgaattgattctatcgaatggcatgagagtttgctataagagtacagactttcttgatgatcaggttatctttacagggttctcatatgggggtttatccgaacttgttgaaagtgagtacttttcttgctcgatggggccgactattgcaggagaaattggggtatatggttatagaccttcagttctgatggatatgcttgcgggtaagaggtccgaagtcagcacaaagcttggagcatacacgagaacctttgctggtgattgttcaccctcagacttagaaactgccttgcagcttgtgtatcaactatttaccacaaacgtaacaccaggagaagaagatgtcaaaatagtgatgcaaatggcagaagaaatggtccgtaatcaagacagggatccttatactgcatttgcaaatcgtgtgaaggagctgaactatggaaactcctatttctttaggcctaccagaataagtgatcttcagaaagttgatccaatgaaagcttgtgaatatttcaacaaatgtttcaaggatccatcaaccttctctatggtcattgttgggaatattgatccttctatagcacttcctttaatattacagtatttgggcggaataccaaagccttctgaacctctcatgcaatataaccgcgatgacctcacaggactgccattcacttttcctagaaccataataagagaagtggtgcgcagccctatggtggaagaacaatgttctgtccagctttgctttcctgtggagctgaataatggaaccatggtagaagatattcacgttgttggtttcctgagcaaacttcttgaaacgaaaataatgcaagttctccggttcaagcatggacagatctacactgtgggtgtttcagtatttcttggcggtaataagccttccagaactgctaatgttcgtggagatataagcataaatttttcttgtgatccagaaatttcatcaaagctggtcgaccttactttggatgaaatattgcgtcttcaagaggaagggccttcggctgaggatgtctcaaccatccttgaaattgagcaacgagcccatgaaaatgggattcaggaaaattactactggctggagaggattttacacagctaccagtcaaggatttactctggcgatgttggcacttgttttgagactcaagaggaaggtcggttaaaagttagacaatctctgaccccaagaacggcgcagttatcattacaaagcatactaccttatccttgcaaaaaacactgtgtcattaagacaattgaaccacgagcttctgcaatagtagaagatttgaaaaatgttgtctctaaaatcagtgctcttgaggaagaaagaatcatttctccatgggatgaagaacatattccagaagaaattgttagcacaaagcctaatccaggagtttggaatcttatatacttactccaaaacacacgacgcgtcgtgcgagtagacaacgcgggtcgtatggacccttataaaaggtgtacaaaactagggtttagcgtctcttgttatattgctctcgcttccgcctctctctcattctgcgcatcgcttcaaggtagggttgaagaagaggagagtgtgaaaatggttgagaagactaagggacgaaaggaggaggtcgtcaccagggagttcaccatctctacactagttattagcttgaatattgtgcagcaatgtgaatattcaaatattggagctactgaattgattctatcgaatggcatgagagtttgctacaagagtacagactttcttgatgatcaggttatctttacagggttctcatatgggggtttatccgaacttgttgaaagtgagtacttttcttgctcgatggggccgactattgcaggagaaattggggtatatggttatagaccttcagttctgatggatatgcttgcgggtaagaggtccgaagtcagcacaaagcttggagcatacacgagaacctttgctggtgattgttcaccctcagacttagaaactgccttgcagcttgtgtatcaactatttaccacaaacgtaacaccaggagaagaagatgtcaaaatagtgatgcaaatggcagaagaaatggtccgtaatcaagacagggatccttatactgcatttgcaaatcgtgtgaaggagctgaactatggaaactcctatttctttaggcctaccagaataagtgatcttcagaaagttgatccaatgaaagcttgtgaatatttcaacaaatgtttcaaggatccatcaaccttctctatggtcattgttgggaatattgatccttctatagcacttcctttaatattacagtatttgggcggaataccaaagccttctgaacctctcatgcaatataaccgcgatgacctcacaggactgccattcacttttcctagaaccataataagccctatggtggaagaacaatgttctgtccagctttgctttcctgtggagctgaataatggaaccatggtagaagatattcacgttgttggtttcctgagcaaacttcttgaaacgaaaataatgcaagttctccggttcaagcatggacagatctacactgtgggtgtttcagtatttcttggcggtaataagccttccagaactgctaatgttcgtggagatataagcataaatttttcttgtgatccagaaatttcatcaaagctggtcgaccttactttggatgaaatattgcgtcttcaagaggaagggccttcggctgaggatgtctcaaccatccttgaaattgagcaacgagcccatgaaaatgggattcaggaaaattactactggctggagaggattttacacagctaccagtcaaggatttactctggcgatgttggcacttgttttgagactcaagaggaaggtcggttaaaagttagacaatctctgaccccaagaacggcgcagttatcattacaaagcatactaccttatccttgcaaaaaacagtacactgtagtaatcttaatgccccgtacttctcggtgtaagttactagattcaatctttcgatctacgaccagttttggtagagatgctaagattttagccgcggttgttggtctcgcagttttgggtcttagcttgtggagacgttcacgaagtacttcttctcatctcaaaactgtagaggaggtccgtagtagcatgaatttctgtagaaccagaattgatacaggaggggagttaatattcttttcttgtactgtcgtgaataacagcacattcaagaagaaggctcccaatgccatcaaggagatcaggaagtttgcccagaaggcaatgggaacaattgatgtcagggtggatgtgaagctgaacaagcaaatctggagcagaggaatcaggagtgtcccaagaagaatcagggttcgcattgctcgcaagaggaatgatgatgaagatgcaaaggaagagctatactcccttgtgactgttgcagagatcccagctgaaggactgaagggcttgggcaccaaggtcattgaagaggatgattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PITH Peptidase_M16 Peptidase_M16_C

Protein Analysis

1728

Amino Acids

194.62

Weight (kDa)

5.38

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 44 - 85 9.3e-09 Insulinase (Peptidase family M16)
Peptidase_M16 PF00675 86 - 187 4.8e-18 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 225 - 405 5.7e-17 Peptidase M16 inactive domain
Peptidase_M16_C PF05193 711 - 894 5.3e-19 Peptidase M16 inactive domain
Peptidase_M16_C PF05193 1294 - 1472 6.8e-17 Peptidase M16 inactive domain
Ribosomal_L31e PF01198 1633 - 1700 2.5e-29 Ribosomal protein L31e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3138
AasI GACNNNNNNGTC 1 cut(s) 74
Acc16I TGCGCA 3 cut(s) 597, 2339, 3211
Acc65I GGTACC 1 cut(s) 711
AccB1I GGYRCC 2 cut(s) 711, 5159
AccB7I CCANNNNNTGG 1 cut(s) 61
AccI GTMKAC 3 cut(s) 2613, 3111, 4347
AccII CGCG 6 cut(s) 1254, 2280, 3099, 3119, 4029, 4739
AcsI RAATTY 7 cut(s) 377, 488, 2575, 2595, 4309, 4329, 4837
AcuI CTGAAG 7 cut(s) 50, 1788, 2090, 3537, 3839, 5160, 5168
AflIII ACRYGT 1 cut(s) 3097
AhlI ACTAGT 1 cut(s) 3316
AjnI CCWGG 6 cut(s) 1163, 1576, 1954, 3055, 3293, 3703
AjuI GAANNNNNNNTTGG 4 cut(s) 245, 277, 1305, 1337
AloI GAACNNNNNNTCC 2 cut(s) 661, 693
Alw21I GWGCWC 2 cut(s) 1501, 2980
AoxI GGCC 8 cut(s) 468, 1134, 1760, 2084, 2654, 3509, 3833, 4388
ApeKI GCWGC 8 cut(s) 152, 1376, 1419, 1591, 1918, 2340, 3340, 3667
ApoI RAATTY 7 cut(s) 377, 488, 2575, 2595, 4309, 4329, 4837
Asp718I GGTACC 1 cut(s) 711
AspLEI GCGC 7 cut(s) 386, 542, 598, 2340, 2857, 3212, 4591
AsuC2I CCSGG 2 cut(s) 16, 755
AsuHPI GGTGA 7 cut(s) 49, 1884, 1895, 3283, 3295, 3633, 3644
AsuII TTCGAA 1 cut(s) 667
AvaII GGWCC 7 cut(s) 52, 1836, 2002, 3130, 3585, 3751, 4822
BaeGI GKGCMC 1 cut(s) 5162
BamHI GGATCC 4 cut(s) 2020, 2158, 3769, 3907
BanI GGYRCC 2 cut(s) 711, 5159
BanII GRGCYC 2 cut(s) 2704, 4438
BarI GAAGNNNNNNTAC 4 cut(s) 2521, 2553, 4255, 4287
BauI CACGAG 4 cut(s) 1446, 1867, 2925, 3616
BbsI GAAGAC 4 cut(s) 1358, 2633, 3269, 4367
Bbv12I GWGCWC 2 cut(s) 1501, 2980
BbvCI CCTCAGC 2 cut(s) 2664, 4398
BbvI GCAGC 8 cut(s) 164, 1388, 1431, 1603, 1930, 2352, 3352, 3679
BceAI ACGGC 3 cut(s) 1187, 2868, 4602
BcgI CGANNNNNNTGC 6 cut(s) 387, 421, 2446, 2480, 4180, 4214
BciT130I CCWGG 6 cut(s) 1165, 1578, 1956, 3057, 3295, 3705
BciVI GTATCC 1 cut(s) 751
BclI TGATCA 2 cut(s) 1681, 3430
BcnI CCSGG 2 cut(s) 16, 755
BcuI ACTAGT 1 cut(s) 3316
BfaI CTAG 8 cut(s) 1073, 1139, 1272, 2313, 3155, 3317, 4062, 4682
BfmI CTRYAG 9 cut(s) 741, 903, 958, 1203, 2205, 3954, 4641, 4812, 4843
BfoI RGCGCY 2 cut(s) 387, 543
BfuI GTATCC 1 cut(s) 751
BglI GCCNNNNNGGC 1 cut(s) 4964
BglII AGATCT 2 cut(s) 2493, 4227
BisI GCNGC 9 cut(s) 153, 1377, 1420, 1592, 1919, 2341, 3341, 3668, 4737
BlpI GCTNAGC 1 cut(s) 633
BlsI GCNGC 9 cut(s) 154, 1378, 1421, 1593, 1920, 2342, 3342, 3669, 4738
BmcAI AGTACT 3 cut(s) 1742, 3491, 4792
Bme1390I CCNGG 8 cut(s) 16, 755, 1165, 1578, 1956, 3057, 3295, 3705
Bme18I GGWCC 7 cut(s) 52, 1836, 2002, 3130, 3585, 3751, 4822
BmrFI CCNGG 8 cut(s) 16, 755, 1165, 1578, 1956, 3057, 3295, 3705
BmrI ACTGGG 1 cut(s) 906
BmuI ACTGGG 1 cut(s) 906
BpiI GAAGAC 4 cut(s) 1358, 2633, 3269, 4367
BplI GAGNNNNNCTC 8 cut(s) 139, 171, 2657, 2689, 4391, 4423, 5093, 5125
BpmI CTGGAG 5 cut(s) 912, 1331, 2759, 4493, 5038
Bpu10I CCTNAGC 5 cut(s) 816, 2438, 2664, 4172, 4398
Bpu1102I GCTNAGC 1 cut(s) 633
Bpu14I TTCGAA 1 cut(s) 667
BpuEI CTTGAG 5 cut(s) 1039, 1523, 2790, 3002, 4524
BpuMI CCSGG 2 cut(s) 16, 755
BsaI GGTCTC 1 cut(s) 4754
BsaJI CCNNGG 9 cut(s) 1164, 1526, 1577, 2406, 3005, 3294, 4140, 4737, 5163
BsaWI WCCGGW 2 cut(s) 2475, 4209
BsaXI ACNNNNNCTCC 2 cut(s) 661, 691
Bse1I ACTGG 7 cut(s) 901, 975, 2738, 2761, 4472, 4495, 4707
Bse3DI GCAATG 4 cut(s) 1601, 3350, 4974, 5061
BseBI CCWGG 6 cut(s) 1165, 1578, 1956, 3057, 3295, 3705
BseDI CCNNGG 9 cut(s) 1164, 1526, 1577, 2406, 3005, 3294, 4140, 4737, 5163
BseMI GCAATG 4 cut(s) 1601, 3350, 4974, 5061
BseNI ACTGG 7 cut(s) 901, 975, 2738, 2761, 4472, 4495, 4707
BseRI GAGGAG 7 cut(s) 209, 222, 284, 297, 3254, 3296, 4832
BseSI GKGCMC 1 cut(s) 5162
BseXI GCAGC 8 cut(s) 164, 1388, 1431, 1603, 1930, 2352, 3352, 3679
BseYI CCCAGC 1 cut(s) 5134
BsgI GTGCAG 3 cut(s) 795, 1610, 3359
Bsh1236I CGCG 6 cut(s) 1254, 2280, 3099, 3119, 4029, 4739
BshFI GGCC 8 cut(s) 470, 1136, 1762, 2086, 2656, 3511, 3835, 4390
BshNI GGYRCC 2 cut(s) 711, 5159
BsiHKAI GWGCWC 2 cut(s) 1501, 2980
BsiSI CCGG 4 cut(s) 15, 755, 2476, 4210
BslFI GGGAC 3 cut(s) 968, 3285, 5024
BsmBI CGTCTC 2 cut(s) 3172, 4771
BsmFI GGGAC 3 cut(s) 968, 3285, 5024
BsmI GAATGC 1 cut(s) 400
BsnI GGCC 8 cut(s) 470, 1136, 1762, 2086, 2656, 3511, 3835, 4390
Bso31I GGTCTC 1 cut(s) 4754
Bsp119I TTCGAA 1 cut(s) 667
Bsp1286I GDGCHC 5 cut(s) 1501, 2704, 2980, 4438, 5162
Bsp1407I TGTACA 1 cut(s) 3146
Bsp1720I GCTNAGC 1 cut(s) 633
Bsp19I CCATGG 4 cut(s) 1526, 2406, 3005, 4140
BspANI GGCC 8 cut(s) 470, 1136, 1762, 2086, 2656, 3511, 3835, 4390
BspFNI CGCG 6 cut(s) 1254, 2280, 3099, 3119, 4029, 4739
BspHI TCATGA 2 cut(s) 340, 853
BspMAI CTGCAG 1 cut(s) 907
BspQI GCTCTTC 2 cut(s) 852, 5094
BspT104I TTCGAA 1 cut(s) 667
BspT107I GGYRCC 2 cut(s) 711, 5159
BspTNI GGTCTC 1 cut(s) 4754
BsrDI GCAATG 4 cut(s) 1601, 3350, 4974, 5061
BsrGI TGTACA 1 cut(s) 3146
BsrI ACTGG 7 cut(s) 901, 975, 2738, 2761, 4472, 4495, 4707
BssECI CCNNGG 9 cut(s) 1164, 1526, 1577, 2406, 3005, 3294, 4140, 4737, 5163
BssSI CACGAG 4 cut(s) 1446, 1867, 2925, 3616
BssT1I CCWWGG 5 cut(s) 1526, 2406, 3005, 4140, 5163
Bst2BI CACGAG 4 cut(s) 1446, 1867, 2925, 3616
Bst2UI CCWGG 6 cut(s) 1165, 1578, 1956, 3057, 3295, 3705
Bst6I CTCTTC 7 cut(s) 186, 261, 546, 852, 3231, 5094, 5169
BstAPI GCANNNNNTGC 1 cut(s) 158
BstAUI TGTACA 1 cut(s) 3146
BstBI TTCGAA 1 cut(s) 667
BstC8I GCNNGC 6 cut(s) 95, 388, 1277, 1824, 3573, 5068
BstDSI CCRYGG 5 cut(s) 1526, 2406, 3005, 4140, 4737
BstENI CCTNNNNNAGG 2 cut(s) 1575, 3054
BstFNI CGCG 6 cut(s) 1254, 2280, 3099, 3119, 4029, 4739
BstH2I RGCGCY 2 cut(s) 387, 543
BstHHI GCGC 7 cut(s) 386, 542, 598, 2340, 2857, 3212, 4591
BstNI CCWGG 6 cut(s) 1165, 1578, 1956, 3057, 3295, 3705
BstSCI CCNGG 8 cut(s) 14, 753, 1163, 1576, 1954, 3055, 3293, 3703
BstSFI CTRYAG 9 cut(s) 741, 903, 958, 1203, 2205, 3954, 4641, 4812, 4843
BstSLI GKGCMC 1 cut(s) 5162
BstUI CGCG 6 cut(s) 1254, 2280, 3099, 3119, 4029, 4739
BstV1I GCAGC 8 cut(s) 164, 1388, 1431, 1603, 1930, 2352, 3352, 3679
BstV2I GAAGAC 4 cut(s) 1358, 2633, 3269, 4367
BstX2I RGATCY 8 cut(s) 1033, 2020, 2158, 2493, 3769, 3907, 4227, 5130
BstXI CCANNNNNNTGG 3 cut(s) 2711, 3063, 4445
BstYI RGATCY 8 cut(s) 1033, 2020, 2158, 2493, 3769, 3907, 4227, 5130
BsuI GTATCC 1 cut(s) 751
BsuRI GGCC 8 cut(s) 470, 1136, 1762, 2086, 2656, 3511, 3835, 4390
BtgI CCRYGG 5 cut(s) 1526, 2406, 3005, 4140, 4737
BtgZI GCGATG 5 cut(s) 2295, 2796, 3198, 4044, 4530
BtsI GCAGTG 3 cut(s) 405, 738, 912
Cac8I GCNNGC 6 cut(s) 95, 388, 1277, 1824, 3573, 5068
CciI TCATGA 2 cut(s) 340, 853
CfoI GCGC 7 cut(s) 386, 542, 598, 2340, 2857, 3212, 4591
Cfr42I CCGCGG 1 cut(s) 4740
CseI GACGC 6 cut(s) 809, 2627, 3088, 3105, 3154, 4361
DraI TTTAAA 1 cut(s) 1015
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
Eam1104I CTCTTC 7 cut(s) 186, 261, 546, 852, 3231, 5094, 5169
EarI CTCTTC 7 cut(s) 186, 261, 546, 852, 3231, 5094, 5169
EciI GGCGGA 4 cut(s) 500, 2253, 3181, 4002
Eco130I CCWWGG 5 cut(s) 1526, 2406, 3005, 4140, 5163
Eco147I AGGCCT 2 cut(s) 2086, 3835
Eco24I GRGCYC 2 cut(s) 2704, 4438
Eco31I GGTCTC 1 cut(s) 4754
Eco32I GATATC 1 cut(s) 1386
Eco47I GGWCC 7 cut(s) 52, 1836, 2002, 3130, 3585, 3751, 4822
Eco57I CTGAAG 7 cut(s) 50, 1788, 2090, 3537, 3839, 5160, 5168
EcoNI CCTNNNNNAGG 2 cut(s) 1575, 3054
EcoO109I RGGNCCY 3 cut(s) 1135, 2654, 4388
EcoRI GAATTC 1 cut(s) 488
EcoRII CCWGG 6 cut(s) 1163, 1576, 1954, 3055, 3293, 3703
EcoRV GATATC 1 cut(s) 1386
EcoT14I CCWWGG 5 cut(s) 1526, 2406, 3005, 4140, 5163
EcoT38I GRGCYC 2 cut(s) 2704, 4438
ErhI CCWWGG 5 cut(s) 1526, 2406, 3005, 4140, 5163
Esp3I CGTCTC 2 cut(s) 3172, 4771
FalI AAGNNNNNCTT 2 cut(s) 4780, 4812
FaqI GGGAC 3 cut(s) 968, 3285, 5024
FauI CCCGC 3 cut(s) 1819, 3112, 3568
FauNDI CATATG 2 cut(s) 1708, 3457
FbaI TGATCA 2 cut(s) 1681, 3430
FblI GTMKAC 3 cut(s) 2613, 3111, 4347
Fnu4HI GCNGC 9 cut(s) 153, 1377, 1420, 1592, 1919, 2341, 3341, 3668, 4737
FriOI GRGCYC 2 cut(s) 2704, 4438
Fsp4HI GCNGC 9 cut(s) 153, 1377, 1420, 1592, 1919, 2341, 3341, 3668, 4737
FspAI RTGCGCAY 1 cut(s) 597
FspBI CTAG 8 cut(s) 1073, 1139, 1272, 2313, 3155, 3317, 4062, 4682
FspI TGCGCA 3 cut(s) 597, 2339, 3211
GlaI GCGC 7 cut(s) 385, 541, 597, 2339, 2856, 3211, 4590
GluI GCNGC 9 cut(s) 153, 1377, 1420, 1592, 1919, 2341, 3341, 3668, 4737
GsaI CCCAGC 1 cut(s) 5138
GsuI CTGGAG 5 cut(s) 912, 1331, 2759, 4493, 5038
HaeII RGCGCY 2 cut(s) 387, 543
HaeIII GGCC 8 cut(s) 470, 1136, 1762, 2086, 2656, 3511, 3835, 4390
HapII CCGG 4 cut(s) 15, 755, 2476, 4210
HgaI GACGC 6 cut(s) 809, 2627, 3088, 3105, 3154, 4361
HhaI GCGC 7 cut(s) 386, 542, 598, 2340, 2857, 3212, 4591
Hin6I GCGC 7 cut(s) 384, 540, 596, 2338, 2855, 3210, 4589
HinP1I GCGC 7 cut(s) 384, 540, 596, 2338, 2855, 3210, 4589
HincII GTYRAC 5 cut(s) 85, 445, 1269, 2614, 4348
HindII GTYRAC 5 cut(s) 85, 445, 1269, 2614, 4348
HindIII AAGCTT 4 cut(s) 1853, 2127, 3602, 3876
HpaII CCGG 4 cut(s) 15, 755, 2476, 4210
HphI GGTGA 7 cut(s) 49, 1884, 1895, 3283, 3295, 3633, 3644
Hpy99I CGWCG 3 cut(s) 80, 3099, 3104
HpyCH4IV ACGT 6 cut(s) 865, 1947, 2424, 3696, 4158, 4780
HpySE526I ACGT 6 cut(s) 865, 1947, 2424, 3696, 4158, 4780
HspAI GCGC 7 cut(s) 384, 540, 596, 2338, 2855, 3210, 4589
KpnI GGTACC 1 cut(s) 715
Ksp22I TGATCA 2 cut(s) 1681, 3430
KspI CCGCGG 1 cut(s) 4740
LguI GCTCTTC 2 cut(s) 852, 5094
Lsp1109I GCAGC 8 cut(s) 164, 1388, 1431, 1603, 1930, 2352, 3352, 3679
MaeI CTAG 8 cut(s) 1073, 1139, 1272, 2313, 3155, 3317, 4062, 4682
MaeII ACGT 6 cut(s) 865, 1947, 2424, 3696, 4158, 4780
MaeIII GTNAC 8 cut(s) 24, 400, 1404, 1948, 3289, 3697, 4677, 5116
MfeI CAATTG 3 cut(s) 1437, 2916, 4977
MflI RGATCY 8 cut(s) 1033, 2020, 2158, 2493, 3769, 3907, 4227, 5130
MhlI GDGCHC 5 cut(s) 1501, 2704, 2980, 4438, 5162
MluI ACGCGT 1 cut(s) 3097
MlyI GAGTC 3 cut(s) 1151, 2797, 4531
MmeI TCCRAC 1 cut(s) 58
MslI CAYNNNNRTG 3 cut(s) 47, 2709, 4443
MspA1I CMGCKG 4 cut(s) 932, 1422, 4739, 5138
MspI CCGG 4 cut(s) 15, 755, 2476, 4210
MspR9I CCNGG 8 cut(s) 16, 755, 1165, 1578, 1956, 3057, 3295, 3705
MunI CAATTG 3 cut(s) 1437, 2916, 4977
Mva1269I GAATGC 1 cut(s) 400
MvaI CCWGG 6 cut(s) 1165, 1578, 1956, 3057, 3295, 3705
MvnI CGCG 6 cut(s) 1254, 2280, 3099, 3119, 4029, 4739
NciI CCSGG 2 cut(s) 16, 755
NcoI CCATGG 4 cut(s) 1526, 2406, 3005, 4140
NdeI CATATG 2 cut(s) 1708, 3457
NmuCI GTSAC 4 cut(s) 24, 400, 3289, 5116
NsbI TGCGCA 3 cut(s) 597, 2339, 3211
NspV TTCGAA 1 cut(s) 667
PagI TCATGA 2 cut(s) 340, 853
PceI AGGCCT 2 cut(s) 2086, 3835
PciSI GCTCTTC 2 cut(s) 852, 5094
PcsI WCGNNNNNNNCGW 3 cut(s) 1760, 3103, 3509
PctI GAATGC 1 cut(s) 400
PflMI CCANNNNNTGG 1 cut(s) 61
PfoI TCCNGGA 2 cut(s) 753, 3055
PkrI GCNGC 9 cut(s) 154, 1378, 1421, 1593, 1920, 2342, 3342, 3669, 4738
PleI GAGTC 3 cut(s) 1150, 2797, 4531
PpsI GAGTC 3 cut(s) 1150, 2797, 4531
PsiI TTATAA 1 cut(s) 3138
Psp6I CCWGG 6 cut(s) 1163, 1576, 1954, 3055, 3293, 3703
PspFI CCCAGC 1 cut(s) 5134
PspGI CCWGG 6 cut(s) 1163, 1576, 1954, 3055, 3293, 3703
PstI CTGCAG 1 cut(s) 907
PsuI RGATCY 8 cut(s) 1033, 2020, 2158, 2493, 3769, 3907, 4227, 5130
PvuII CAGCTG 3 cut(s) 932, 1422, 5138
RseI CAYNNNNRTG 3 cut(s) 47, 2709, 4443
SacII CCGCGG 1 cut(s) 4740
SalI GTCGAC 2 cut(s) 2612, 4346
SapI GCTCTTC 2 cut(s) 852, 5094
SatI GCNGC 9 cut(s) 153, 1377, 1420, 1592, 1919, 2341, 3341, 3668, 4737
ScaI AGTACT 3 cut(s) 1742, 3491, 4792
SchI GAGTC 3 cut(s) 1151, 2797, 4531
ScrFI CCNGG 8 cut(s) 16, 755, 1165, 1578, 1956, 3057, 3295, 3705
SduI GDGCHC 5 cut(s) 1501, 2704, 2980, 4438, 5162
SfcI CTRYAG 9 cut(s) 741, 903, 958, 1203, 2205, 3954, 4641, 4812, 4843
Sfr303I CCGCGG 1 cut(s) 4740
SfuI TTCGAA 1 cut(s) 667
SgrBI CCGCGG 1 cut(s) 4740
SinI GGWCC 7 cut(s) 52, 1836, 2002, 3130, 3585, 3751, 4822
SmiMI CAYNNNNRTG 3 cut(s) 47, 2709, 4443
SmlI CTYRAG 5 cut(s) 1054, 1502, 2805, 2981, 4539
SmoI CTYRAG 5 cut(s) 1054, 1502, 2805, 2981, 4539
SpeI ACTAGT 1 cut(s) 3316
SseBI AGGCCT 2 cut(s) 2086, 3835
SspMI CTAG 8 cut(s) 1073, 1139, 1272, 2313, 3155, 3317, 4062, 4682
StuI AGGCCT 2 cut(s) 2086, 3835
StyD4I CCNGG 8 cut(s) 14, 753, 1163, 1576, 1954, 3055, 3293, 3703
StyI CCWWGG 5 cut(s) 1526, 2406, 3005, 4140, 5163
TaiI ACGT 6 cut(s) 868, 1950, 2427, 3699, 4161, 4783
TauI GCSGC 1 cut(s) 4739
TseFI GTSAC 4 cut(s) 24, 400, 3289, 5116
TseI GCWGC 8 cut(s) 152, 1376, 1419, 1591, 1918, 2340, 3340, 3667
Tsp45I GTSAC 4 cut(s) 24, 400, 3289, 5116
TspGWI ACGGA 4 cut(s) 1295, 1994, 3743, 4814
Van91I CCANNNNNTGG 1 cut(s) 61
VpaK11BI GGWCC 7 cut(s) 52, 1836, 2002, 3130, 3585, 3751, 4822
XagI CCTNNNNNAGG 2 cut(s) 1575, 3054
XapI RAATTY 7 cut(s) 377, 488, 2575, 2595, 4309, 4329, 4837
XcmI CCANNNNNNNNNTGG 1 cut(s) 478
XmiI GTMKAC 3 cut(s) 2613, 3111, 4347
XspI CTAG 8 cut(s) 1073, 1139, 1272, 2313, 3155, 3317, 4062, 4682
ZrmI AGTACT 3 cut(s) 1742, 3491, 4792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.