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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G57570 AT3G57570 |
| fragaria_vesca | FvH4_2g02670 FvH4_2g02670 FvH4_2g02670 FvH4_2g02670 FvH4_2g02670 FvH4_2g02670 FvH4_7g07942 FvH4_7g07943 |
| malus_domestica | MD05G1111300.v1.1 |
| prunus_persica | Prupe.8G156400_v2.0.a1 Prupe.8G156400_v2.0.a1 |
| pyrus_communis | pycom05g10710 |
| rosa_chinensis | RchiOBHm_Chr6g0247071 RchiOBHm_Chr7g0177161 |
| rosa_laevigata | RLG00000015224 |
| rosa_multiflora | Rmu_sc0002476.1_g000020 Rmu_sc0004577.1_g000007 Rmu_sc0005310.1_g000018 Rmu_sc0027214.1_g000001 |
| rosa_roxburghii | Rroxscaffold_7G00214360 |
| rosa_rugosa | Rorug05G0522000.1 |
| rosa_samantha | Rh6AG037100 Rh6BG031800 Rh6CG033000 Rh6CG033100 Rh6DG030800 Rh7AG003700 Rh7CG003400 |
| rosa_wichuraiana | Rw6G003180 Rw7G000300 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 3248 |
| AatII | GACGTC | 1 cut(s) | 80 |
| Acc36I | ACCTGC | 4 cut(s) | 1154, 1774, 2937, 3248 |
| AccB1I | GGYRCC | 1 cut(s) | 2977 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 340 |
| AccBSI | CCGCTC | 1 cut(s) | 251 |
| AccII | CGCG | 1 cut(s) | 151 |
| AclWI | GGATC | 6 cut(s) | 879, 1140, 2816, 3207, 3244, 3362 |
| AcoI | YGGCCR | 3 cut(s) | 91, 331, 770 |
| AcsI | RAATTY | 4 cut(s) | 276, 1103, 1256, 2504 |
| AcuI | CTGAAG | 1 cut(s) | 1884 |
| AcvI | CACGTG | 1 cut(s) | 2899 |
| AcyI | GRCGYC | 3 cut(s) | 77, 141, 246 |
| AdeI | CACNNNGTG | 1 cut(s) | 555 |
| AfaI | GTAC | 8 cut(s) | 454, 462, 900, 1308, 1641, 3139, 3164, 3345 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 150, 340, 509, 1234, 1944, 3007 |
| AflII | CTTAAG | 1 cut(s) | 965 |
| AjiI | CACGTC | 2 cut(s) | 2652, 3086 |
| AjnI | CCWGG | 4 cut(s) | 333, 1390, 2004, 2689 |
| Alw21I | GWGCWC | 7 cut(s) | 100, 488, 1842, 2175, 2202, 2770, 2898 |
| Alw44I | GTGCAC | 1 cut(s) | 2894 |
| AlwI | GGATC | 6 cut(s) | 879, 1140, 2816, 3207, 3244, 3362 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 824, 2186, 2468, 3050 |
| Ama87I | CYCGRG | 1 cut(s) | 2742 |
| AoxI | GGCC | 8 cut(s) | 91, 181, 331, 608, 770, 786, 2668, 2735 |
| ApaLI | GTGCAC | 1 cut(s) | 2894 |
| ApoI | RAATTY | 4 cut(s) | 276, 1103, 1256, 2504 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2102, 2134 |
| AseI | ATTAAT | 2 cut(s) | 1370, 1742 |
| AspLEI | GCGC | 2 cut(s) | 258, 1554 |
| AspS9I | GGNCC | 3 cut(s) | 182, 609, 787 |
| AsuC2I | CCSGG | 2 cut(s) | 1792, 2672 |
| AsuHPI | GGTGA | 8 cut(s) | 54, 812, 1380, 1396, 1439, 2876, 3118, 3366 |
| AsuII | TTCGAA | 1 cut(s) | 381 |
| AsuNHI | GCTAGC | 1 cut(s) | 3406 |
| AvaI | CYCGRG | 1 cut(s) | 2742 |
| BaeGI | GKGCMC | 1 cut(s) | 2898 |
| BanI | GGYRCC | 1 cut(s) | 2977 |
| BanII | GRGCYC | 1 cut(s) | 488 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1299, 1331 |
| BauI | CACGAG | 2 cut(s) | 977, 2891 |
| BbrPI | CACGTG | 1 cut(s) | 2899 |
| BbsI | GAAGAC | 1 cut(s) | 1047 |
| Bbv12I | GWGCWC | 7 cut(s) | 100, 488, 1842, 2175, 2202, 2770, 2898 |
| BbvCI | CCTCAGC | 1 cut(s) | 1011 |
| BceAI | ACGGC | 3 cut(s) | 128, 343, 650 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 371, 405 |
| BciT130I | CCWGG | 4 cut(s) | 335, 1392, 2006, 2691 |
| BciVI | GTATCC | 2 cut(s) | 537, 2452 |
| BclI | TGATCA | 2 cut(s) | 708, 2901 |
| BcnI | CCSGG | 2 cut(s) | 1792, 2672 |
| BfaI | CTAG | 6 cut(s) | 896, 1454, 1476, 2259, 3288, 3407 |
| BfoI | RGCGCY | 1 cut(s) | 1555 |
| BfrI | CTTAAG | 1 cut(s) | 965 |
| BfuAI | ACCTGC | 4 cut(s) | 1154, 1774, 2937, 3248 |
| BfuI | GTATCC | 2 cut(s) | 537, 2452 |
| BglII | AGATCT | 2 cut(s) | 3074, 3338 |
| BlpI | GCTNAGC | 2 cut(s) | 2532, 2811 |
| Bme1390I | CCNGG | 6 cut(s) | 335, 1392, 1792, 2006, 2672, 2691 |
| BmeT110I | CYCGRG | 1 cut(s) | 2742 |
| BmgBI | CACGTC | 2 cut(s) | 2652, 3086 |
| BmgT120I | GGNCC | 3 cut(s) | 182, 609, 787 |
| BmiI | GGNNCC | 4 cut(s) | 6, 184, 202, 2979 |
| BmrFI | CCNGG | 6 cut(s) | 335, 1392, 1792, 2006, 2672, 2691 |
| BmrI | ACTGGG | 2 cut(s) | 1244, 2578 |
| BmtI | GCTAGC | 1 cut(s) | 3410 |
| BmuI | ACTGGG | 2 cut(s) | 1244, 2578 |
| BoxI | GACNNNNGTC | 1 cut(s) | 80 |
| BpiI | GAAGAC | 1 cut(s) | 1047 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 1004, 1036, 1279, 1311, 2933, 2965 |
| BpmI | CTGGAG | 1 cut(s) | 1988 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1011, 2045 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 2532, 2811 |
| Bpu14I | TTCGAA | 1 cut(s) | 381 |
| BpuEI | CTTGAG | 2 cut(s) | 2541, 3126 |
| BpuMI | CCSGG | 2 cut(s) | 1792, 2672 |
| Bsa29I | ATCGAT | 1 cut(s) | 2784 |
| BsaAI | YACGTR | 2 cut(s) | 464, 2899 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1807 |
| BsaHI | GRCGYC | 3 cut(s) | 77, 141, 246 |
| BsaI | GGTCTC | 3 cut(s) | 1190, 1953, 3282 |
| BsaJI | CCNNGG | 3 cut(s) | 393, 1791, 3195 |
| BsaWI | WCCGGW | 2 cut(s) | 64, 1412 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 891, 921 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 150, 340, 509, 1234, 1944, 3007 |
| Bse118I | RCCGGY | 2 cut(s) | 179, 772 |
| Bse1I | ACTGG | 7 cut(s) | 359, 1042, 1239, 1728, 1818, 2584, 2981 |
| Bse3DI | GCAATG | 2 cut(s) | 1436, 2333 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1807 |
| BseBI | CCWGG | 4 cut(s) | 335, 1392, 2006, 2691 |
| BseCI | ATCGAT | 1 cut(s) | 2784 |
| BseDI | CCNNGG | 3 cut(s) | 393, 1791, 3195 |
| BseJI | GATNNNNATC | 1 cut(s) | 1807 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 150, 340, 509, 1234, 1944, 3007 |
| BseMI | GCAATG | 2 cut(s) | 1436, 2333 |
| BseNI | ACTGG | 7 cut(s) | 359, 1042, 1239, 1728, 1818, 2584, 2981 |
| BseRI | GAGGAG | 4 cut(s) | 94, 150, 218, 1594 |
| BseSI | GKGCMC | 1 cut(s) | 2898 |
| BseX3I | CGGCCG | 2 cut(s) | 91, 770 |
| BseYI | CCCAGC | 2 cut(s) | 1110, 1647 |
| Bsh1236I | CGCG | 1 cut(s) | 151 |
| Bsh1285I | CGRYCG | 3 cut(s) | 94, 773, 2038 |
| BshFI | GGCC | 8 cut(s) | 93, 183, 333, 610, 772, 788, 2670, 2737 |
| BshNI | GGYRCC | 1 cut(s) | 2977 |
| BshVI | ATCGAT | 1 cut(s) | 2784 |
| BsiEI | CGRYCG | 3 cut(s) | 94, 773, 2038 |
| BsiHKAI | GWGCWC | 7 cut(s) | 100, 488, 1842, 2175, 2202, 2770, 2898 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2742 |
| BsiSI | CCGG | 6 cut(s) | 65, 180, 773, 1413, 1791, 2671 |
| BslFI | GGGAC | 2 cut(s) | 2638, 2860 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 150, 340, 509, 1234, 1944, 3007 |
| BsmBI | CGTCTC | 2 cut(s) | 199, 3092 |
| BsmFI | GGGAC | 2 cut(s) | 2638, 2860 |
| BsmI | GAATGC | 3 cut(s) | 558, 1591, 1864 |
| BsnI | GGCC | 8 cut(s) | 93, 183, 333, 610, 772, 788, 2670, 2737 |
| Bso31I | GGTCTC | 3 cut(s) | 1190, 1953, 3282 |
| BsoBI | CYCGRG | 1 cut(s) | 2742 |
| Bsp119I | TTCGAA | 1 cut(s) | 381 |
| Bsp1286I | GDGCHC | 7 cut(s) | 100, 488, 1842, 2175, 2202, 2770, 2898 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 2532, 2811 |
| Bsp19I | CCATGG | 2 cut(s) | 393, 3195 |
| BspANI | GGCC | 8 cut(s) | 93, 183, 333, 610, 772, 788, 2670, 2737 |
| BspDI | ATCGAT | 1 cut(s) | 2784 |
| BspFNI | CGCG | 1 cut(s) | 151 |
| BspHI | TCATGA | 1 cut(s) | 2425 |
| BspLI | GGNNCC | 4 cut(s) | 6, 184, 202, 2979 |
| BspMI | ACCTGC | 4 cut(s) | 1154, 1774, 2937, 3248 |
| BspOI | GCTAGC | 1 cut(s) | 3410 |
| BspPI | GGATC | 6 cut(s) | 879, 1140, 2816, 3207, 3244, 3362 |
| BspQI | GCTCTTC | 2 cut(s) | 2478, 3231 |
| BspT104I | TTCGAA | 1 cut(s) | 381 |
| BspT107I | GGYRCC | 1 cut(s) | 2977 |
| BspTI | CTTAAG | 1 cut(s) | 965 |
| BspTNI | GGTCTC | 3 cut(s) | 1190, 1953, 3282 |
| BsrBI | CCGCTC | 1 cut(s) | 251 |
| BsrDI | GCAATG | 2 cut(s) | 1436, 2333 |
| BsrFI | RCCGGY | 2 cut(s) | 179, 772 |
| BsrI | ACTGG | 7 cut(s) | 359, 1042, 1239, 1728, 1818, 2584, 2981 |
| BssAI | RCCGGY | 2 cut(s) | 179, 772 |
| BssECI | CCNNGG | 3 cut(s) | 393, 1791, 3195 |
| BssNI | GRCGYC | 3 cut(s) | 77, 141, 246 |
| BssSI | CACGAG | 2 cut(s) | 977, 2891 |
| BssT1I | CCWWGG | 2 cut(s) | 393, 3195 |
| Bst2BI | CACGAG | 2 cut(s) | 977, 2891 |
| Bst2UI | CCWGG | 4 cut(s) | 335, 1392, 2006, 2691 |
| Bst4CI | ACNGT | 9 cut(s) | 1053, 1155, 1201, 1526, 1889, 2103, 2432, 2464, 3348 |
| Bst6I | CTCTTC | 4 cut(s) | 114, 2478, 3084, 3231 |
| BstACI | GRCGYC | 3 cut(s) | 77, 141, 246 |
| BstAFI | CTTAAG | 1 cut(s) | 965 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 2186, 2787 |
| BstBAI | YACGTR | 2 cut(s) | 464, 2899 |
| BstBI | TTCGAA | 1 cut(s) | 381 |
| BstC8I | GCNNGC | 8 cut(s) | 121, 1018, 1079, 1147, 2220, 3031, 3408, 3412 |
| BstDSI | CCRYGG | 2 cut(s) | 393, 3195 |
| BstFNI | CGCG | 1 cut(s) | 151 |
| BstH2I | RGCGCY | 1 cut(s) | 1555 |
| BstHHI | GCGC | 2 cut(s) | 258, 1554 |
| BstMCI | CGRYCG | 3 cut(s) | 94, 773, 2038 |
| BstNI | CCWGG | 4 cut(s) | 335, 1392, 2006, 2691 |
| BstNSI | RCATGY | 5 cut(s) | 944, 1081, 2222, 3138, 3414 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 80 |
| BstSCI | CCNGG | 6 cut(s) | 333, 1390, 1790, 2004, 2670, 2689 |
| BstSLI | GKGCMC | 1 cut(s) | 2898 |
| BstUI | CGCG | 1 cut(s) | 151 |
| BstV2I | GAAGAC | 1 cut(s) | 1047 |
| BstX2I | RGATCY | 5 cut(s) | 2821, 3074, 3199, 3338, 3367 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 400, 952 |
| BstYI | RGATCY | 5 cut(s) | 2821, 3074, 3199, 3338, 3367 |
| BstZI | CGGCCG | 2 cut(s) | 91, 770 |
| Bsu15I | ATCGAT | 1 cut(s) | 2784 |
| BsuI | GTATCC | 2 cut(s) | 537, 2452 |
| BsuRI | GGCC | 8 cut(s) | 93, 183, 333, 610, 772, 788, 2670, 2737 |
| BsuTUI | ATCGAT | 1 cut(s) | 2784 |
| BtgI | CCRYGG | 2 cut(s) | 393, 3195 |
| BtrI | CACGTC | 2 cut(s) | 2652, 3086 |
| BtsIMutI | CAGTG | 4 cut(s) | 1170, 1535, 1825, 3031 |
| BveI | ACCTGC | 4 cut(s) | 1154, 1774, 2937, 3248 |
| Cac8I | GCNNGC | 8 cut(s) | 121, 1018, 1079, 1147, 2220, 3031, 3408, 3412 |
| CaiI | CAGNNNCTG | 4 cut(s) | 824, 2186, 2468, 3050 |
| CciI | TCATGA | 1 cut(s) | 2425 |
| CfoI | GCGC | 2 cut(s) | 258, 1554 |
| Cfr10I | RCCGGY | 2 cut(s) | 179, 772 |
| Cfr13I | GGNCC | 3 cut(s) | 182, 609, 787 |
| ClaI | ATCGAT | 1 cut(s) | 2784 |
| CseI | GACGC | 2 cut(s) | 149, 254 |
| Csp6I | GTAC | 8 cut(s) | 453, 461, 899, 1307, 1640, 3138, 3163, 3344 |
| CviQI | GTAC | 8 cut(s) | 453, 461, 899, 1307, 1640, 3138, 3163, 3344 |
| DraIII | CACNNNGTG | 1 cut(s) | 555 |
| EaeI | YGGCCR | 3 cut(s) | 91, 331, 770 |
| EagI | CGGCCG | 2 cut(s) | 91, 770 |
| Eam1104I | CTCTTC | 4 cut(s) | 114, 2478, 3084, 3231 |
| EarI | CTCTTC | 4 cut(s) | 114, 2478, 3084, 3231 |
| EciI | GGCGGA | 2 cut(s) | 25, 106 |
| Ecl136II | GAGCTC | 1 cut(s) | 486 |
| EclXI | CGGCCG | 2 cut(s) | 91, 770 |
| Eco130I | CCWWGG | 2 cut(s) | 393, 3195 |
| Eco24I | GRGCYC | 1 cut(s) | 488 |
| Eco31I | GGTCTC | 3 cut(s) | 1190, 1953, 3282 |
| Eco32I | GATATC | 3 cut(s) | 1293, 2440, 3042 |
| Eco52I | CGGCCG | 2 cut(s) | 91, 770 |
| Eco53kI | GAGCTC | 1 cut(s) | 486 |
| Eco57I | CTGAAG | 1 cut(s) | 1884 |
| Eco72I | CACGTG | 1 cut(s) | 2899 |
| Eco88I | CYCGRG | 1 cut(s) | 2742 |
| EcoICRI | GAGCTC | 1 cut(s) | 486 |
| EcoRI | GAATTC | 1 cut(s) | 2504 |
| EcoRII | CCWGG | 4 cut(s) | 333, 1390, 2004, 2689 |
| EcoRV | GATATC | 3 cut(s) | 1293, 2440, 3042 |
| EcoT14I | CCWWGG | 2 cut(s) | 393, 3195 |
| EcoT22I | ATGCAT | 4 cut(s) | 1089, 2220, 2783, 2969 |
| EcoT38I | GRGCYC | 1 cut(s) | 488 |
| ErhI | CCWWGG | 2 cut(s) | 393, 3195 |
| Esp3I | CGTCTC | 2 cut(s) | 199, 3092 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 583, 615, 1912, 1944 |
| FaqI | GGGAC | 2 cut(s) | 2638, 2860 |
| FauI | CCCGC | 3 cut(s) | 128, 193, 1947 |
| FauNDI | CATATG | 1 cut(s) | 1617 |
| FbaI | TGATCA | 2 cut(s) | 708, 2901 |
| FriOI | GRGCYC | 1 cut(s) | 488 |
| FspBI | CTAG | 6 cut(s) | 896, 1454, 1476, 2259, 3288, 3407 |
| GlaI | GCGC | 2 cut(s) | 257, 1553 |
| GsaI | CCCAGC | 2 cut(s) | 1114, 1651 |
| GsuI | CTGGAG | 1 cut(s) | 1988 |
| HaeII | RGCGCY | 1 cut(s) | 1555 |
| HaeIII | GGCC | 8 cut(s) | 93, 183, 333, 610, 772, 788, 2670, 2737 |
| HapII | CCGG | 6 cut(s) | 65, 180, 773, 1413, 1791, 2671 |
| HgaI | GACGC | 2 cut(s) | 149, 254 |
| HhaI | GCGC | 2 cut(s) | 258, 1554 |
| Hin1I | GRCGYC | 3 cut(s) | 77, 141, 246 |
| Hin6I | GCGC | 2 cut(s) | 256, 1552 |
| HinP1I | GCGC | 2 cut(s) | 256, 1552 |
| HincII | GTYRAC | 1 cut(s) | 2402 |
| HindII | GTYRAC | 1 cut(s) | 2402 |
| HindIII | AAGCTT | 1 cut(s) | 2628 |
| HpaII | CCGG | 6 cut(s) | 65, 180, 773, 1413, 1791, 2671 |
| HphI | GGTGA | 8 cut(s) | 54, 812, 1380, 1396, 1439, 2876, 3118, 3366 |
| Hpy166II | GTNNAC | 4 cut(s) | 453, 2402, 2896, 3163 |
| Hpy8I | GTNNAC | 4 cut(s) | 453, 2402, 2896, 3163 |
| Hpy99I | CGWCG | 7 cut(s) | 46, 143, 212, 248, 275, 421, 2232 |
| HpyAV | CCTTC | 9 cut(s) | 415, 663, 1018, 1238, 1460, 1761, 2748, 3238, 3381 |
| HpyCH4III | ACNGT | 9 cut(s) | 1053, 1155, 1201, 1526, 1889, 2103, 2432, 2464, 3348 |
| HpyCH4IV | ACGT | 9 cut(s) | 77, 463, 1002, 1638, 2230, 2651, 2898, 3085, 3385 |
| HpySE526I | ACGT | 9 cut(s) | 77, 463, 1002, 1638, 2230, 2651, 2898, 3085, 3385 |
| Hsp92I | GRCGYC | 3 cut(s) | 77, 141, 246 |
| HspAI | GCGC | 2 cut(s) | 256, 1552 |
| Ksp22I | TGATCA | 2 cut(s) | 708, 2901 |
| LguI | GCTCTTC | 2 cut(s) | 2478, 3231 |
| LmnI | GCTCC | 6 cut(s) | 138, 206, 826, 918, 955, 2585 |
| MaeI | CTAG | 6 cut(s) | 896, 1454, 1476, 2259, 3288, 3407 |
| MaeII | ACGT | 9 cut(s) | 77, 463, 1002, 1638, 2230, 2651, 2898, 3085, 3385 |
| MaeIII | GTNAC | 7 cut(s) | 82, 490, 731, 775, 998, 1166, 2464 |
| MbiI | CCGCTC | 1 cut(s) | 251 |
| MfeI | CAATTG | 1 cut(s) | 573 |
| MflI | RGATCY | 5 cut(s) | 2821, 3074, 3199, 3338, 3367 |
| MhlI | GDGCHC | 7 cut(s) | 100, 488, 1842, 2175, 2202, 2770, 2898 |
| MlyI | GAGTC | 9 cut(s) | 50, 77, 206, 311, 1905, 2167, 2285, 2951, 3041 |
| MmeI | TCCRAC | 6 cut(s) | 24, 1043, 1110, 1597, 2157, 2970 |
| Mph1103I | ATGCAT | 4 cut(s) | 1089, 2220, 2783, 2969 |
| MslI | CAYNNNNRTG | 5 cut(s) | 287, 1082, 1616, 2166, 3104 |
| MspA1I | CMGCKG | 2 cut(s) | 2359, 3158 |
| MspCI | CTTAAG | 1 cut(s) | 965 |
| MspI | CCGG | 6 cut(s) | 65, 180, 773, 1413, 1791, 2671 |
| MspR9I | CCNGG | 6 cut(s) | 335, 1392, 1792, 2006, 2672, 2691 |
| MunI | CAATTG | 1 cut(s) | 573 |
| Mva1269I | GAATGC | 3 cut(s) | 558, 1591, 1864 |
| MvaI | CCWGG | 4 cut(s) | 335, 1392, 2006, 2691 |
| MvnI | CGCG | 1 cut(s) | 151 |
| NciI | CCSGG | 2 cut(s) | 1792, 2672 |
| NcoI | CCATGG | 2 cut(s) | 393, 3195 |
| NdeI | CATATG | 1 cut(s) | 1617 |
| NheI | GCTAGC | 1 cut(s) | 3406 |
| NlaIV | GGNNCC | 4 cut(s) | 6, 184, 202, 2979 |
| NmeAIII | GCCGAG | 3 cut(s) | 69, 119, 232 |
| NmuCI | GTSAC | 2 cut(s) | 82, 1166 |
| NsiI | ATGCAT | 4 cut(s) | 1089, 2220, 2783, 2969 |
| NspI | RCATGY | 5 cut(s) | 944, 1081, 2222, 3138, 3414 |
| NspV | TTCGAA | 1 cut(s) | 381 |
| PaeI | GCATGC | 3 cut(s) | 1081, 2222, 3414 |
| PagI | TCATGA | 1 cut(s) | 2425 |
| PaqCI | CACCTGC | 1 cut(s) | 3248 |
| PciSI | GCTCTTC | 2 cut(s) | 2478, 3231 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 267, 3091 |
| PctI | GAATGC | 3 cut(s) | 558, 1591, 1864 |
| PfeI | GAWTC | 9 cut(s) | 752, 827, 1444, 1628, 1925, 2381, 2675, 2828, 2857 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 340 |
| Ple19I | CGATCG | 1 cut(s) | 2038 |
| PleI | GAGTC | 9 cut(s) | 50, 76, 206, 311, 1904, 2166, 2285, 2950, 3041 |
| PmaCI | CACGTG | 1 cut(s) | 2899 |
| PmlI | CACGTG | 1 cut(s) | 2899 |
| PpsI | GAGTC | 9 cut(s) | 50, 76, 206, 311, 1904, 2166, 2285, 2950, 3041 |
| Ppu21I | YACGTR | 2 cut(s) | 464, 2899 |
| PshAI | GACNNNNGTC | 1 cut(s) | 80 |
| PshBI | ATTAAT | 2 cut(s) | 1370, 1742 |
| Psp124BI | GAGCTC | 1 cut(s) | 488 |
| Psp6I | CCWGG | 4 cut(s) | 333, 1390, 2004, 2689 |
| PspCI | CACGTG | 1 cut(s) | 2899 |
| PspFI | CCCAGC | 2 cut(s) | 1110, 1647 |
| PspGI | CCWGG | 4 cut(s) | 333, 1390, 2004, 2689 |
| PspN4I | GGNNCC | 4 cut(s) | 6, 184, 202, 2979 |
| PspPI | GGNCC | 3 cut(s) | 182, 609, 787 |
| PstNI | CAGNNNCTG | 4 cut(s) | 824, 2186, 2468, 3050 |
| PsuI | RGATCY | 5 cut(s) | 2821, 3074, 3199, 3338, 3367 |
| PvuI | CGATCG | 1 cut(s) | 2038 |
| PvuII | CAGCTG | 1 cut(s) | 3158 |
| RsaI | GTAC | 8 cut(s) | 454, 462, 900, 1308, 1641, 3139, 3164, 3345 |
| RsaNI | GTAC | 8 cut(s) | 453, 461, 899, 1307, 1640, 3138, 3163, 3344 |
| RseI | CAYNNNNRTG | 5 cut(s) | 287, 1082, 1616, 2166, 3104 |
| SacI | GAGCTC | 1 cut(s) | 488 |
| SapI | GCTCTTC | 2 cut(s) | 2478, 3231 |
| Sau96I | GGNCC | 3 cut(s) | 182, 609, 787 |
| SchI | GAGTC | 9 cut(s) | 50, 77, 206, 311, 1905, 2167, 2285, 2951, 3041 |
| ScrFI | CCNGG | 6 cut(s) | 335, 1392, 1792, 2006, 2672, 2691 |
| SduI | GDGCHC | 7 cut(s) | 100, 488, 1842, 2175, 2202, 2770, 2898 |
| SfuI | TTCGAA | 1 cut(s) | 381 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 287, 1082, 1616, 2166, 3104 |
| SmlI | CTYRAG | 3 cut(s) | 965, 2556, 3141 |
| SmoI | CTYRAG | 3 cut(s) | 965, 2556, 3141 |
| SphI | GCATGC | 3 cut(s) | 1081, 2222, 3414 |
| SspI | AATATT | 2 cut(s) | 1044, 1374 |
| SspMI | CTAG | 6 cut(s) | 896, 1454, 1476, 2259, 3288, 3407 |
| SstI | GAGCTC | 1 cut(s) | 488 |
| StyD4I | CCNGG | 6 cut(s) | 333, 1390, 1790, 2004, 2670, 2689 |
| StyI | CCWWGG | 2 cut(s) | 393, 3195 |
| TaaI | ACNGT | 9 cut(s) | 1053, 1155, 1201, 1526, 1889, 2103, 2432, 2464, 3348 |
| TaiI | ACGT | 9 cut(s) | 80, 466, 1005, 1641, 2233, 2654, 2901, 3088, 3388 |
| TatI | WGTACW | 4 cut(s) | 452, 898, 3137, 3343 |
| TauI | GCSGC | 4 cut(s) | 154, 251, 333, 772 |
| TfiI | GAWTC | 9 cut(s) | 752, 827, 1444, 1628, 1925, 2381, 2675, 2828, 2857 |
| TscAI | CASTG | 4 cut(s) | 1177, 1535, 1825, 3031 |
| TseFI | GTSAC | 2 cut(s) | 82, 1166 |
| Tsp45I | GTSAC | 2 cut(s) | 82, 1166 |
| TspDTI | ATGAA | 9 cut(s) | 521, 660, 1307, 1473, 1743, 1812, 2414, 2540, 2769 |
| TspGWI | ACGGA | 1 cut(s) | 181 |
| TspRI | CASTG | 4 cut(s) | 1177, 1535, 1825, 3031 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 340 |
| Vha464I | CTTAAG | 1 cut(s) | 965 |
| VneI | GTGCAC | 1 cut(s) | 2894 |
| VspI | ATTAAT | 2 cut(s) | 1370, 1742 |
| XapI | RAATTY | 4 cut(s) | 276, 1103, 1256, 2504 |
| XbaI | TCTAGA | 2 cut(s) | 1453, 2258 |
| XceI | RCATGY | 5 cut(s) | 944, 1081, 2222, 3138, 3414 |
| XspI | CTAG | 6 cut(s) | 896, 1454, 1476, 2259, 3288, 3407 |
| ZraI | GACGTC | 1 cut(s) | 78 |
| Zsp2I | ATGCAT | 4 cut(s) | 1089, 2220, 2783, 2969 |