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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G57570 AT3G57570 |
| fragaria_vesca | FvH4_2g02670 FvH4_2g02670 FvH4_2g02670 FvH4_2g02670 FvH4_2g02670 FvH4_2g02670 FvH4_7g07942 FvH4_7g07943 |
| malus_domestica | MD05G1111300.v1.1 |
| prunus_persica | Prupe.8G156400_v2.0.a1 Prupe.8G156400_v2.0.a1 |
| pyrus_communis | pycom05g10710 |
| rosa_chinensis | RchiOBHm_Chr6g0247071 RchiOBHm_Chr7g0177161 |
| rosa_laevigata | RLG00000015224 |
| rosa_multiflora | Rmu_sc0002476.1_g000020 Rmu_sc0004577.1_g000007 Rmu_sc0005310.1_g000018 Rmu_sc0027214.1_g000001 |
| rosa_roxburghii | Rroxscaffold_7G00214360 |
| rosa_rugosa | Rorug05G0522000.1 |
| rosa_samantha | Rh6AG037100 Rh6BG031800 Rh6CG033000 Rh6CG033100 Rh6DG030800 Rh7AG003700 Rh7CG003400 |
| rosa_wichuraiana | Rw6G003180 Rw7G000300 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2972 |
| AatII | GACGTC | 1 cut(s) | 80 |
| Acc36I | ACCTGC | 4 cut(s) | 968, 1588, 2661, 2972 |
| AccB1I | GGYRCC | 1 cut(s) | 2701 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 340 |
| AccBSI | CCGCTC | 2 cut(s) | 251, 2195 |
| AccII | CGCG | 3 cut(s) | 151, 2820, 3213 |
| AccIII | TCCGGA | 1 cut(s) | 3296 |
| AclWI | GGATC | 6 cut(s) | 846, 954, 1750, 2540, 2931, 2968 |
| AcoI | YGGCCR | 8 cut(s) | 91, 181, 331, 737, 3167, 3208, 3221, 3249 |
| AcsI | RAATTY | 4 cut(s) | 917, 1070, 2228, 3236 |
| AcuI | CTGAAG | 1 cut(s) | 1698 |
| AcvI | CACGTG | 1 cut(s) | 2623 |
| AcyI | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| AfaI | GTAC | 7 cut(s) | 454, 462, 867, 1122, 1455, 2863, 2888 |
| AgeI | ACCGGT | 1 cut(s) | 3185 |
| AhlI | ACTAGT | 1 cut(s) | 3268 |
| AjiI | CACGTC | 2 cut(s) | 2376, 2810 |
| AjnI | CCWGG | 3 cut(s) | 333, 1204, 2413 |
| Alw21I | GWGCWC | 7 cut(s) | 488, 1656, 1899, 1926, 2262, 2494, 2622 |
| Alw44I | GTGCAC | 1 cut(s) | 2618 |
| AlwI | GGATC | 6 cut(s) | 846, 954, 1750, 2540, 2931, 2968 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 791, 1910, 2774 |
| Aor13HI | TCCGGA | 1 cut(s) | 3296 |
| ApaLI | GTGCAC | 1 cut(s) | 2618 |
| ApoI | RAATTY | 4 cut(s) | 917, 1070, 2228, 3236 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1826, 1858 |
| AseI | ATTAAT | 3 cut(s) | 1184, 1556, 3086 |
| AsiGI | ACCGGT | 1 cut(s) | 3185 |
| AspLEI | GCGC | 2 cut(s) | 1368, 2822 |
| AspS9I | GGNCC | 1 cut(s) | 754 |
| AsuC2I | CCSGG | 2 cut(s) | 1606, 2396 |
| AsuII | TTCGAA | 1 cut(s) | 381 |
| BaeGI | GKGCMC | 1 cut(s) | 2622 |
| BanI | GGYRCC | 1 cut(s) | 2701 |
| BanII | GRGCYC | 3 cut(s) | 488, 2262, 3159 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1113, 1145 |
| BauI | CACGAG | 2 cut(s) | 2615, 3132 |
| BbrPI | CACGTG | 1 cut(s) | 2623 |
| Bbv12I | GWGCWC | 7 cut(s) | 488, 1656, 1899, 1926, 2262, 2494, 2622 |
| BbvCI | CCTCAGC | 1 cut(s) | 1715 |
| BceAI | ACGGC | 4 cut(s) | 128, 343, 617, 3239 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 371, 405 |
| BciT130I | CCWGG | 3 cut(s) | 335, 1206, 2415 |
| BciVI | GTATCC | 2 cut(s) | 537, 2176 |
| BclI | TGATCA | 2 cut(s) | 675, 2625 |
| BcnI | CCSGG | 2 cut(s) | 1606, 2396 |
| BcuI | ACTAGT | 1 cut(s) | 3268 |
| BfaI | CTAG | 8 cut(s) | 863, 1268, 1290, 1983, 2249, 3012, 3054, 3269 |
| BfoI | RGCGCY | 1 cut(s) | 1369 |
| BfuAI | ACCTGC | 4 cut(s) | 968, 1588, 2661, 2972 |
| BfuI | GTATCC | 2 cut(s) | 537, 2176 |
| BglII | AGATCT | 1 cut(s) | 2798 |
| BlpI | GCTNAGC | 2 cut(s) | 2256, 2535 |
| Bme1390I | CCNGG | 5 cut(s) | 335, 1206, 1606, 2396, 2415 |
| BmgBI | CACGTC | 2 cut(s) | 2376, 2810 |
| BmgT120I | GGNCC | 1 cut(s) | 754 |
| BmiI | GGNNCC | 4 cut(s) | 6, 202, 2703, 3156 |
| BmrFI | CCNGG | 5 cut(s) | 335, 1206, 1606, 2396, 2415 |
| BmrI | ACTGGG | 2 cut(s) | 1058, 2302 |
| BmsI | GCATC | 9 cut(s) | 574, 1228, 1547, 1627, 2064, 2492, 2514, 2581, 3010 |
| BmuI | ACTGGG | 2 cut(s) | 1058, 2302 |
| BoxI | GACNNNNGTC | 1 cut(s) | 80 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1093, 1125 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1715, 1769 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 2256, 2535 |
| Bpu14I | TTCGAA | 1 cut(s) | 381 |
| BpuEI | CTTGAG | 2 cut(s) | 2265, 2850 |
| BpuMI | CCSGG | 2 cut(s) | 1606, 2396 |
| Bsa29I | ATCGAT | 1 cut(s) | 2508 |
| BsaAI | YACGTR | 2 cut(s) | 464, 2623 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1621 |
| BsaHI | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| BsaI | GGTCTC | 2 cut(s) | 1004, 3006 |
| BsaJI | CCNNGG | 4 cut(s) | 393, 1605, 2919, 3170 |
| BsaWI | WCCGGW | 7 cut(s) | 64, 1226, 3178, 3185, 3192, 3240, 3296 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 858, 888 |
| Bse118I | RCCGGY | 5 cut(s) | 179, 739, 3165, 3185, 3206 |
| Bse1I | ACTGG | 8 cut(s) | 359, 1053, 1542, 1632, 2308, 2705, 3200, 3252 |
| Bse3DI | GCAATG | 2 cut(s) | 1250, 2057 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1621 |
| BseAI | TCCGGA | 1 cut(s) | 3296 |
| BseBI | CCWGG | 3 cut(s) | 335, 1206, 2415 |
| BseCI | ATCGAT | 1 cut(s) | 2508 |
| BseDI | CCNNGG | 4 cut(s) | 393, 1605, 2919, 3170 |
| BseJI | GATNNNNATC | 1 cut(s) | 1621 |
| BseMI | GCAATG | 2 cut(s) | 1250, 2057 |
| BseNI | ACTGG | 8 cut(s) | 359, 1053, 1542, 1632, 2308, 2705, 3200, 3252 |
| BseRI | GAGGAG | 5 cut(s) | 94, 101, 150, 218, 1408 |
| BseSI | GKGCMC | 1 cut(s) | 2622 |
| BseX3I | CGGCCG | 3 cut(s) | 91, 737, 3208 |
| BseYI | CCCAGC | 2 cut(s) | 924, 1461 |
| BsgI | GTGCAG | 1 cut(s) | 566 |
| Bsh1236I | CGCG | 3 cut(s) | 151, 2820, 3213 |
| Bsh1285I | CGRYCG | 4 cut(s) | 94, 740, 1762, 3211 |
| BshNI | GGYRCC | 1 cut(s) | 2701 |
| BshTI | ACCGGT | 1 cut(s) | 3185 |
| BshVI | ATCGAT | 1 cut(s) | 2508 |
| BsiEI | CGRYCG | 4 cut(s) | 94, 740, 1762, 3211 |
| BsiHKAI | GWGCWC | 7 cut(s) | 488, 1656, 1899, 1926, 2262, 2494, 2622 |
| BslFI | GGGAC | 2 cut(s) | 2362, 2584 |
| BsmBI | CGTCTC | 2 cut(s) | 199, 2816 |
| BsmFI | GGGAC | 2 cut(s) | 2362, 2584 |
| BsmI | GAATGC | 3 cut(s) | 558, 1405, 1678 |
| Bso31I | GGTCTC | 2 cut(s) | 1004, 3006 |
| Bsp119I | TTCGAA | 1 cut(s) | 381 |
| Bsp1286I | GDGCHC | 8 cut(s) | 488, 1656, 1899, 1926, 2262, 2494, 2622, 3159 |
| Bsp13I | TCCGGA | 1 cut(s) | 3296 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 2256, 2535 |
| Bsp19I | CCATGG | 2 cut(s) | 393, 2919 |
| BspDI | ATCGAT | 1 cut(s) | 2508 |
| BspEI | TCCGGA | 1 cut(s) | 3296 |
| BspFNI | CGCG | 3 cut(s) | 151, 2820, 3213 |
| BspHI | TCATGA | 1 cut(s) | 2149 |
| BspLI | GGNNCC | 4 cut(s) | 6, 202, 2703, 3156 |
| BspMI | ACCTGC | 4 cut(s) | 968, 1588, 2661, 2972 |
| BspPI | GGATC | 6 cut(s) | 846, 954, 1750, 2540, 2931, 2968 |
| BspQI | GCTCTTC | 2 cut(s) | 2202, 2955 |
| BspT104I | TTCGAA | 1 cut(s) | 381 |
| BspT107I | GGYRCC | 1 cut(s) | 2701 |
| BspTNI | GGTCTC | 2 cut(s) | 1004, 3006 |
| BsrBI | CCGCTC | 2 cut(s) | 251, 2195 |
| BsrDI | GCAATG | 2 cut(s) | 1250, 2057 |
| BsrFI | RCCGGY | 5 cut(s) | 179, 739, 3165, 3185, 3206 |
| BsrI | ACTGG | 8 cut(s) | 359, 1053, 1542, 1632, 2308, 2705, 3200, 3252 |
| BssAI | RCCGGY | 5 cut(s) | 179, 739, 3165, 3185, 3206 |
| BssECI | CCNNGG | 4 cut(s) | 393, 1605, 2919, 3170 |
| BssNI | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| BssSI | CACGAG | 2 cut(s) | 2615, 3132 |
| BssT1I | CCWWGG | 2 cut(s) | 393, 2919 |
| Bst2BI | CACGAG | 2 cut(s) | 2615, 3132 |
| Bst2UI | CCWGG | 3 cut(s) | 335, 1206, 2415 |
| Bst4CI | ACNGT | 6 cut(s) | 969, 1340, 1703, 1827, 2156, 2188 |
| Bst6I | CTCTTC | 3 cut(s) | 2202, 2808, 2955 |
| BstACI | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1910, 2511 |
| BstBAI | YACGTR | 2 cut(s) | 464, 2623 |
| BstBI | TTCGAA | 1 cut(s) | 381 |
| BstC8I | GCNNGC | 5 cut(s) | 121, 893, 961, 1944, 2755 |
| BstDSI | CCRYGG | 3 cut(s) | 393, 2919, 3170 |
| BstEII | GGTNACC | 2 cut(s) | 3181, 3188 |
| BstFNI | CGCG | 3 cut(s) | 151, 2820, 3213 |
| BstH2I | RGCGCY | 1 cut(s) | 1369 |
| BstHHI | GCGC | 2 cut(s) | 1368, 2822 |
| BstMCI | CGRYCG | 4 cut(s) | 94, 740, 1762, 3211 |
| BstNI | CCWGG | 3 cut(s) | 335, 1206, 2415 |
| BstNSI | RCATGY | 3 cut(s) | 895, 1946, 2862 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 80 |
| BstPI | GGTNACC | 2 cut(s) | 3181, 3188 |
| BstSCI | CCNGG | 5 cut(s) | 333, 1204, 1604, 2394, 2413 |
| BstSLI | GKGCMC | 1 cut(s) | 2622 |
| BstUI | CGCG | 3 cut(s) | 151, 2820, 3213 |
| BstX2I | RGATCY | 3 cut(s) | 2545, 2798, 2923 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 400, 3090 |
| BstYI | RGATCY | 3 cut(s) | 2545, 2798, 2923 |
| BstZI | CGGCCG | 3 cut(s) | 91, 737, 3208 |
| Bsu15I | ATCGAT | 1 cut(s) | 2508 |
| BsuI | GTATCC | 2 cut(s) | 537, 2176 |
| BsuTUI | ATCGAT | 1 cut(s) | 2508 |
| BtgI | CCRYGG | 3 cut(s) | 393, 2919, 3170 |
| BtrI | CACGTC | 2 cut(s) | 2376, 2810 |
| BtsIMutI | CAGTG | 6 cut(s) | 984, 1349, 1639, 2755, 3112, 3245 |
| BveI | ACCTGC | 4 cut(s) | 968, 1588, 2661, 2972 |
| Cac8I | GCNNGC | 5 cut(s) | 121, 893, 961, 1944, 2755 |
| CaiI | CAGNNNCTG | 3 cut(s) | 791, 1910, 2774 |
| CciI | TCATGA | 1 cut(s) | 2149 |
| CfoI | GCGC | 2 cut(s) | 1368, 2822 |
| Cfr10I | RCCGGY | 5 cut(s) | 179, 739, 3165, 3185, 3206 |
| Cfr13I | GGNCC | 1 cut(s) | 754 |
| ClaI | ATCGAT | 1 cut(s) | 2508 |
| CseI | GACGC | 3 cut(s) | 149, 254, 812 |
| Csp6I | GTAC | 7 cut(s) | 453, 461, 866, 1121, 1454, 2862, 2887 |
| CspAI | ACCGGT | 1 cut(s) | 3185 |
| CviQI | GTAC | 7 cut(s) | 453, 461, 866, 1121, 1454, 2862, 2887 |
| EaeI | YGGCCR | 8 cut(s) | 91, 181, 331, 737, 3167, 3208, 3221, 3249 |
| EagI | CGGCCG | 3 cut(s) | 91, 737, 3208 |
| Eam1104I | CTCTTC | 3 cut(s) | 2202, 2808, 2955 |
| EarI | CTCTTC | 3 cut(s) | 2202, 2808, 2955 |
| EciI | GGCGGA | 2 cut(s) | 25, 106 |
| Ecl136II | GAGCTC | 2 cut(s) | 486, 2260 |
| EclXI | CGGCCG | 3 cut(s) | 91, 737, 3208 |
| Eco130I | CCWWGG | 2 cut(s) | 393, 2919 |
| Eco24I | GRGCYC | 3 cut(s) | 488, 2262, 3159 |
| Eco31I | GGTCTC | 2 cut(s) | 1004, 3006 |
| Eco32I | GATATC | 3 cut(s) | 1107, 2164, 2766 |
| Eco52I | CGGCCG | 3 cut(s) | 91, 737, 3208 |
| Eco53kI | GAGCTC | 2 cut(s) | 486, 2260 |
| Eco57I | CTGAAG | 1 cut(s) | 1698 |
| Eco72I | CACGTG | 1 cut(s) | 2623 |
| Eco91I | GGTNACC | 2 cut(s) | 3181, 3188 |
| EcoICRI | GAGCTC | 2 cut(s) | 486, 2260 |
| EcoO65I | GGTNACC | 2 cut(s) | 3181, 3188 |
| EcoRI | GAATTC | 2 cut(s) | 2228, 3236 |
| EcoRII | CCWGG | 3 cut(s) | 333, 1204, 2413 |
| EcoRV | GATATC | 3 cut(s) | 1107, 2164, 2766 |
| EcoT14I | CCWWGG | 2 cut(s) | 393, 2919 |
| EcoT22I | ATGCAT | 4 cut(s) | 903, 1944, 2507, 2693 |
| EcoT38I | GRGCYC | 3 cut(s) | 488, 2262, 3159 |
| ErhI | CCWWGG | 2 cut(s) | 393, 2919 |
| Esp3I | CGTCTC | 2 cut(s) | 199, 2816 |
| FaqI | GGGAC | 2 cut(s) | 2362, 2584 |
| FauI | CCCGC | 1 cut(s) | 128 |
| FauNDI | CATATG | 1 cut(s) | 1431 |
| FbaI | TGATCA | 2 cut(s) | 675, 2625 |
| FriOI | GRGCYC | 3 cut(s) | 488, 2262, 3159 |
| FspBI | CTAG | 8 cut(s) | 863, 1268, 1290, 1983, 2249, 3012, 3054, 3269 |
| GlaI | GCGC | 2 cut(s) | 1367, 2821 |
| GsaI | CCCAGC | 2 cut(s) | 928, 1465 |
| HaeII | RGCGCY | 1 cut(s) | 1369 |
| HgaI | GACGC | 3 cut(s) | 149, 254, 812 |
| HhaI | GCGC | 2 cut(s) | 1368, 2822 |
| Hin1I | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| Hin6I | GCGC | 2 cut(s) | 1366, 2820 |
| HinP1I | GCGC | 2 cut(s) | 1366, 2820 |
| HincII | GTYRAC | 1 cut(s) | 2126 |
| HindII | GTYRAC | 1 cut(s) | 2126 |
| HindIII | AAGCTT | 1 cut(s) | 2352 |
| Hpy166II | GTNNAC | 4 cut(s) | 453, 2126, 2620, 2887 |
| Hpy8I | GTNNAC | 4 cut(s) | 453, 2126, 2620, 2887 |
| Hpy99I | CGWCG | 5 cut(s) | 46, 143, 212, 248, 1956 |
| HpyAV | CCTTC | 5 cut(s) | 1052, 1274, 1575, 2472, 2962 |
| HpyCH4III | ACNGT | 6 cut(s) | 969, 1340, 1703, 1827, 2156, 2188 |
| HpyCH4IV | ACGT | 7 cut(s) | 77, 463, 1452, 1954, 2375, 2622, 2809 |
| HpySE526I | ACGT | 7 cut(s) | 77, 463, 1452, 1954, 2375, 2622, 2809 |
| Hsp92I | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| HspAI | GCGC | 2 cut(s) | 1366, 2820 |
| Kpn2I | TCCGGA | 1 cut(s) | 3296 |
| Ksp22I | TGATCA | 2 cut(s) | 675, 2625 |
| LguI | GCTCTTC | 2 cut(s) | 2202, 2955 |
| LmnI | GCTCC | 5 cut(s) | 138, 206, 793, 2309, 3154 |
| LweI | GCATC | 9 cut(s) | 574, 1228, 1547, 1627, 2064, 2492, 2514, 2581, 3010 |
| MaeI | CTAG | 8 cut(s) | 863, 1268, 1290, 1983, 2249, 3012, 3054, 3269 |
| MaeII | ACGT | 7 cut(s) | 77, 463, 1452, 1954, 2375, 2622, 2809 |
| MbiI | CCGCTC | 2 cut(s) | 251, 2195 |
| MflI | RGATCY | 3 cut(s) | 2545, 2798, 2923 |
| MhlI | GDGCHC | 8 cut(s) | 488, 1656, 1899, 1926, 2262, 2494, 2622, 3159 |
| MlyI | GAGTC | 8 cut(s) | 50, 77, 206, 311, 1891, 2009, 2675, 2765 |
| MmeI | TCCRAC | 5 cut(s) | 24, 924, 1411, 1881, 2694 |
| Mph1103I | ATGCAT | 4 cut(s) | 903, 1944, 2507, 2693 |
| MroI | TCCGGA | 1 cut(s) | 3296 |
| MslI | CAYNNNNRTG | 5 cut(s) | 287, 896, 1430, 1890, 2828 |
| MspA1I | CMGCKG | 3 cut(s) | 2083, 2882, 3142 |
| MspR9I | CCNGG | 5 cut(s) | 335, 1206, 1606, 2396, 2415 |
| Mva1269I | GAATGC | 3 cut(s) | 558, 1405, 1678 |
| MvaI | CCWGG | 3 cut(s) | 335, 1206, 2415 |
| MvnI | CGCG | 3 cut(s) | 151, 2820, 3213 |
| NciI | CCSGG | 2 cut(s) | 1606, 2396 |
| NcoI | CCATGG | 2 cut(s) | 393, 2919 |
| NdeI | CATATG | 1 cut(s) | 1431 |
| NlaIV | GGNNCC | 4 cut(s) | 6, 202, 2703, 3156 |
| NmeAIII | GCCGAG | 3 cut(s) | 69, 119, 232 |
| NmuCI | GTSAC | 5 cut(s) | 82, 980, 3188, 3202, 3243 |
| NsiI | ATGCAT | 4 cut(s) | 903, 1944, 2507, 2693 |
| NspI | RCATGY | 3 cut(s) | 895, 1946, 2862 |
| NspV | TTCGAA | 1 cut(s) | 381 |
| PaeI | GCATGC | 2 cut(s) | 895, 1946 |
| PagI | TCATGA | 1 cut(s) | 2149 |
| PaqCI | CACCTGC | 1 cut(s) | 2972 |
| PciSI | GCTCTTC | 2 cut(s) | 2202, 2955 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 267 |
| PctI | GAATGC | 3 cut(s) | 558, 1405, 1678 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 340 |
| PinAI | ACCGGT | 1 cut(s) | 3185 |
| Ple19I | CGATCG | 1 cut(s) | 1762 |
| PleI | GAGTC | 8 cut(s) | 50, 76, 206, 311, 1890, 2009, 2674, 2765 |
| PmaCI | CACGTG | 1 cut(s) | 2623 |
| PmlI | CACGTG | 1 cut(s) | 2623 |
| PpsI | GAGTC | 8 cut(s) | 50, 76, 206, 311, 1890, 2009, 2674, 2765 |
| Ppu21I | YACGTR | 2 cut(s) | 464, 2623 |
| PshAI | GACNNNNGTC | 1 cut(s) | 80 |
| PshBI | ATTAAT | 3 cut(s) | 1184, 1556, 3086 |
| Psp124BI | GAGCTC | 2 cut(s) | 488, 2262 |
| Psp6I | CCWGG | 3 cut(s) | 333, 1204, 2413 |
| PspCI | CACGTG | 1 cut(s) | 2623 |
| PspEI | GGTNACC | 2 cut(s) | 3181, 3188 |
| PspFI | CCCAGC | 2 cut(s) | 924, 1461 |
| PspGI | CCWGG | 3 cut(s) | 333, 1204, 2413 |
| PspN4I | GGNNCC | 4 cut(s) | 6, 202, 2703, 3156 |
| PspPI | GGNCC | 1 cut(s) | 754 |
| PstNI | CAGNNNCTG | 3 cut(s) | 791, 1910, 2774 |
| PsuI | RGATCY | 3 cut(s) | 2545, 2798, 2923 |
| PvuI | CGATCG | 1 cut(s) | 1762 |
| PvuII | CAGCTG | 2 cut(s) | 2882, 3142 |
| RsaI | GTAC | 7 cut(s) | 454, 462, 867, 1122, 1455, 2863, 2888 |
| RsaNI | GTAC | 7 cut(s) | 453, 461, 866, 1121, 1454, 2862, 2887 |
| RseI | CAYNNNNRTG | 5 cut(s) | 287, 896, 1430, 1890, 2828 |
| SacI | GAGCTC | 2 cut(s) | 488, 2262 |
| SapI | GCTCTTC | 2 cut(s) | 2202, 2955 |
| Sau96I | GGNCC | 1 cut(s) | 754 |
| SchI | GAGTC | 8 cut(s) | 50, 77, 206, 311, 1891, 2009, 2675, 2765 |
| ScrFI | CCNGG | 5 cut(s) | 335, 1206, 1606, 2396, 2415 |
| SduI | GDGCHC | 8 cut(s) | 488, 1656, 1899, 1926, 2262, 2494, 2622, 3159 |
| SfaNI | GCATC | 9 cut(s) | 574, 1228, 1547, 1627, 2064, 2492, 2514, 2581, 3010 |
| SfuI | TTCGAA | 1 cut(s) | 381 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 287, 896, 1430, 1890, 2828 |
| SmlI | CTYRAG | 2 cut(s) | 2280, 2865 |
| SmoI | CTYRAG | 2 cut(s) | 2280, 2865 |
| SpeI | ACTAGT | 1 cut(s) | 3268 |
| SphI | GCATGC | 2 cut(s) | 895, 1946 |
| SspI | AATATT | 1 cut(s) | 1188 |
| SspMI | CTAG | 8 cut(s) | 863, 1268, 1290, 1983, 2249, 3012, 3054, 3269 |
| SstI | GAGCTC | 2 cut(s) | 488, 2262 |
| StyD4I | CCNGG | 5 cut(s) | 333, 1204, 1604, 2394, 2413 |
| StyI | CCWWGG | 2 cut(s) | 393, 2919 |
| TaaI | ACNGT | 6 cut(s) | 969, 1340, 1703, 1827, 2156, 2188 |
| TaiI | ACGT | 7 cut(s) | 80, 466, 1455, 1957, 2378, 2625, 2812 |
| TatI | WGTACW | 3 cut(s) | 452, 865, 2861 |
| TauI | GCSGC | 6 cut(s) | 154, 251, 333, 739, 3213, 3254 |
| TscAI | CASTG | 6 cut(s) | 991, 1349, 1639, 2755, 3112, 3252 |
| TseFI | GTSAC | 5 cut(s) | 82, 980, 3188, 3202, 3243 |
| Tsp45I | GTSAC | 5 cut(s) | 82, 980, 3188, 3202, 3243 |
| TspGWI | ACGGA | 2 cut(s) | 181, 3187 |
| TspRI | CASTG | 6 cut(s) | 991, 1349, 1639, 2755, 3112, 3252 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 340 |
| VneI | GTGCAC | 1 cut(s) | 2618 |
| VspI | ATTAAT | 3 cut(s) | 1184, 1556, 3086 |
| XapI | RAATTY | 4 cut(s) | 917, 1070, 2228, 3236 |
| XbaI | TCTAGA | 2 cut(s) | 1267, 1982 |
| XceI | RCATGY | 3 cut(s) | 895, 1946, 2862 |
| XspI | CTAG | 8 cut(s) | 863, 1268, 1290, 1983, 2249, 3012, 3054, 3269 |
| ZraI | GACGTC | 1 cut(s) | 78 |
| Zsp2I | ATGCAT | 4 cut(s) | 903, 1944, 2507, 2693 |