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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G57570 AT3G57570 |
| fragaria_vesca | FvH4_2g02670 FvH4_2g02670 FvH4_2g02670 FvH4_2g02670 FvH4_2g02670 FvH4_2g02670 FvH4_7g07942 FvH4_7g07943 |
| malus_domestica | MD05G1111300.v1.1 |
| prunus_persica | Prupe.8G156400_v2.0.a1 Prupe.8G156400_v2.0.a1 |
| pyrus_communis | pycom05g10710 |
| rosa_chinensis | RchiOBHm_Chr6g0247071 RchiOBHm_Chr7g0177161 |
| rosa_laevigata | RLG00000015224 |
| rosa_multiflora | Rmu_sc0002476.1_g000020 Rmu_sc0004577.1_g000007 Rmu_sc0005310.1_g000018 Rmu_sc0027214.1_g000001 |
| rosa_roxburghii | Rroxscaffold_7G00214360 |
| rosa_rugosa | Rorug05G0522000.1 |
| rosa_samantha | Rh6AG037100 Rh6BG031800 Rh6CG033000 Rh6CG033100 Rh6DG030800 Rh7AG003700 Rh7CG003400 |
| rosa_wichuraiana | Rw6G003180 Rw7G000300 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2822 |
| AatII | GACGTC | 1 cut(s) | 80 |
| Acc36I | ACCTGC | 4 cut(s) | 1612, 2538, 2652, 2822 |
| AccB1I | GGYRCC | 1 cut(s) | 2692 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 340 |
| AccBSI | CCGCTC | 1 cut(s) | 251 |
| AccII | CGCG | 2 cut(s) | 151, 2811 |
| AclWI | GGATC | 5 cut(s) | 846, 1039, 1741, 2531, 2936 |
| AcoI | YGGCCR | 2 cut(s) | 91, 331 |
| AcsI | RAATTY | 7 cut(s) | 276, 718, 889, 1002, 1093, 1145, 2219 |
| AcuI | CTGAAG | 2 cut(s) | 326, 1721 |
| AcvI | CACGTG | 1 cut(s) | 2614 |
| AcyI | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| AfaI | GTAC | 5 cut(s) | 454, 462, 867, 1197, 2919 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 150, 340, 509, 1274, 2722 |
| AjiI | CACGTC | 2 cut(s) | 2367, 2801 |
| AjnI | CCWGG | 3 cut(s) | 1255, 1360, 2404 |
| Alw21I | GWGCWC | 7 cut(s) | 488, 1679, 1890, 1917, 2253, 2485, 2613 |
| Alw26I | GTCTC | 7 cut(s) | 199, 1079, 1376, 1485, 2429, 2807, 2856 |
| Alw44I | GTGCAC | 1 cut(s) | 2609 |
| AlwI | GGATC | 5 cut(s) | 846, 1039, 1741, 2531, 2936 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 791, 1901, 2183, 2765 |
| Ama87I | CYCGRG | 1 cut(s) | 319 |
| AoxI | GGCC | 6 cut(s) | 91, 181, 331, 753, 2383, 2450 |
| ApaLI | GTGCAC | 1 cut(s) | 2609 |
| ApeKI | GCWGC | 7 cut(s) | 641, 1295, 1716, 1965, 2243, 2312, 2585 |
| ApoI | RAATTY | 7 cut(s) | 276, 718, 889, 1002, 1093, 1145, 2219 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1817, 1849 |
| AseI | ATTAAT | 1 cut(s) | 1340 |
| AspLEI | GCGC | 2 cut(s) | 1524, 2813 |
| AspS9I | GGNCC | 4 cut(s) | 182, 754, 1253, 1282 |
| AsuC2I | CCSGG | 2 cut(s) | 1630, 2387 |
| AsuHPI | GGTGA | 7 cut(s) | 54, 779, 1350, 1366, 1409, 2591, 2940 |
| AsuII | TTCGAA | 1 cut(s) | 381 |
| AvaI | CYCGRG | 1 cut(s) | 319 |
| AvaII | GGWCC | 2 cut(s) | 1253, 1282 |
| BaeGI | GKGCMC | 1 cut(s) | 2613 |
| BanI | GGYRCC | 1 cut(s) | 2692 |
| BanII | GRGCYC | 2 cut(s) | 488, 2253 |
| BauI | CACGAG | 1 cut(s) | 2606 |
| BbrPI | CACGTG | 1 cut(s) | 2614 |
| BbsI | GAAGAC | 2 cut(s) | 946, 1109 |
| Bbv12I | GWGCWC | 7 cut(s) | 488, 1679, 1890, 1917, 2253, 2485, 2613 |
| BbvI | GCAGC | 7 cut(s) | 628, 1282, 1703, 1977, 2230, 2299, 2572 |
| BccI | CCATC | 9 cut(s) | 599, 755, 1067, 1430, 1852, 1948, 2171, 2456, 2821 |
| BceAI | ACGGC | 3 cut(s) | 128, 343, 617 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 371, 405 |
| BciT130I | CCWGG | 3 cut(s) | 1257, 1362, 2406 |
| BciVI | GTATCC | 2 cut(s) | 537, 2167 |
| BclI | TGATCA | 3 cut(s) | 675, 1240, 2616 |
| BcnI | CCSGG | 2 cut(s) | 1630, 2387 |
| BcoDI | GTCTC | 7 cut(s) | 199, 1079, 1376, 1485, 2429, 2807, 2856 |
| BfaI | CTAG | 7 cut(s) | 863, 1224, 1424, 1446, 1974, 2862, 2981 |
| BfoI | RGCGCY | 1 cut(s) | 1525 |
| BfuAI | ACCTGC | 4 cut(s) | 1612, 2538, 2652, 2822 |
| BfuI | GTATCC | 2 cut(s) | 537, 2167 |
| BglII | AGATCT | 2 cut(s) | 2789, 2912 |
| BlpI | GCTNAGC | 2 cut(s) | 1839, 2247 |
| Bme1390I | CCNGG | 5 cut(s) | 1257, 1362, 1630, 2387, 2406 |
| Bme18I | GGWCC | 2 cut(s) | 1253, 1282 |
| BmeT110I | CYCGRG | 1 cut(s) | 319 |
| BmgBI | CACGTC | 2 cut(s) | 2367, 2801 |
| BmgT120I | GGNCC | 4 cut(s) | 182, 754, 1253, 1282 |
| BmiI | GGNNCC | 5 cut(s) | 6, 184, 202, 1283, 2694 |
| BmrFI | CCNGG | 5 cut(s) | 1257, 1362, 1630, 2387, 2406 |
| BmrI | ACTGGG | 2 cut(s) | 1133, 2293 |
| BmsI | GCATC | 8 cut(s) | 574, 1384, 1650, 2055, 2483, 2505, 2572, 2860 |
| BmuI | ACTGGG | 2 cut(s) | 1133, 2293 |
| BoxI | GACNNNNGTC | 1 cut(s) | 80 |
| BpiI | GAAGAC | 2 cut(s) | 946, 1109 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1168, 1200 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1760 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1839, 2247 |
| Bpu14I | TTCGAA | 1 cut(s) | 381 |
| BpuEI | CTTGAG | 1 cut(s) | 2256 |
| BpuMI | CCSGG | 2 cut(s) | 1630, 2387 |
| Bsa29I | ATCGAT | 1 cut(s) | 2499 |
| BsaAI | YACGTR | 2 cut(s) | 464, 2614 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1644 |
| BsaHI | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| BsaI | GGTCTC | 2 cut(s) | 1079, 2856 |
| BsaJI | CCNNGG | 3 cut(s) | 393, 1256, 1268 |
| BsaWI | WCCGGW | 2 cut(s) | 64, 1382 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 858, 888 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 150, 340, 509, 1274, 2722 |
| Bse118I | RCCGGY | 1 cut(s) | 179 |
| Bse1I | ACTGG | 6 cut(s) | 359, 941, 1128, 1655, 2299, 2696 |
| Bse3DI | GCAATG | 2 cut(s) | 1406, 2048 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1644 |
| BseBI | CCWGG | 3 cut(s) | 1257, 1362, 2406 |
| BseCI | ATCGAT | 1 cut(s) | 2499 |
| BseDI | CCNNGG | 3 cut(s) | 393, 1256, 1268 |
| BseJI | GATNNNNATC | 1 cut(s) | 1644 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 150, 340, 509, 1274, 2722 |
| BseMI | GCAATG | 2 cut(s) | 1406, 2048 |
| BseMII | CTCAG | 8 cut(s) | 210, 1053, 1074, 1199, 1830, 2066, 2238, 2336 |
| BseNI | ACTGG | 6 cut(s) | 359, 941, 1128, 1655, 2299, 2696 |
| BseRI | GAGGAG | 5 cut(s) | 94, 101, 150, 218, 1564 |
| BseSI | GKGCMC | 1 cut(s) | 2613 |
| BseX3I | CGGCCG | 1 cut(s) | 91 |
| BseXI | GCAGC | 7 cut(s) | 628, 1282, 1703, 1977, 2230, 2299, 2572 |
| BseYI | CCCAGC | 1 cut(s) | 1009 |
| BsgI | GTGCAG | 1 cut(s) | 566 |
| Bsh1236I | CGCG | 2 cut(s) | 151, 2811 |
| Bsh1285I | CGRYCG | 2 cut(s) | 94, 1753 |
| BshFI | GGCC | 6 cut(s) | 93, 183, 333, 755, 2385, 2452 |
| BshNI | GGYRCC | 1 cut(s) | 2692 |
| BshVI | ATCGAT | 1 cut(s) | 2499 |
| BsiEI | CGRYCG | 2 cut(s) | 94, 1753 |
| BsiHKAI | GWGCWC | 7 cut(s) | 488, 1679, 1890, 1917, 2253, 2485, 2613 |
| BsiHKCI | CYCGRG | 1 cut(s) | 319 |
| BsiSI | CCGG | 5 cut(s) | 65, 180, 1383, 1629, 2386 |
| BslFI | GGGAC | 3 cut(s) | 1295, 2353, 2575 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 150, 340, 509, 1274, 2722 |
| BsmAI | GTCTC | 7 cut(s) | 199, 1079, 1376, 1485, 2429, 2807, 2856 |
| BsmBI | CGTCTC | 2 cut(s) | 199, 2807 |
| BsmFI | GGGAC | 3 cut(s) | 1295, 2353, 2575 |
| BsmI | GAATGC | 3 cut(s) | 558, 1561, 1701 |
| BsnI | GGCC | 6 cut(s) | 93, 183, 333, 755, 2385, 2452 |
| Bso31I | GGTCTC | 2 cut(s) | 1079, 2856 |
| BsoBI | CYCGRG | 1 cut(s) | 319 |
| Bsp119I | TTCGAA | 1 cut(s) | 381 |
| Bsp1286I | GDGCHC | 7 cut(s) | 488, 1679, 1890, 1917, 2253, 2485, 2613 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1839, 2247 |
| Bsp19I | CCATGG | 1 cut(s) | 393 |
| BspANI | GGCC | 6 cut(s) | 93, 183, 333, 755, 2385, 2452 |
| BspCNI | CTCAG | 8 cut(s) | 209, 1054, 1075, 1198, 1831, 2067, 2239, 2335 |
| BspDI | ATCGAT | 1 cut(s) | 2499 |
| BspFNI | CGCG | 2 cut(s) | 151, 2811 |
| BspHI | TCATGA | 1 cut(s) | 2140 |
| BspLI | GGNNCC | 5 cut(s) | 6, 184, 202, 1283, 2694 |
| BspMI | ACCTGC | 4 cut(s) | 1612, 2538, 2652, 2822 |
| BspPI | GGATC | 5 cut(s) | 846, 1039, 1741, 2531, 2936 |
| BspQI | GCTCTTC | 1 cut(s) | 2193 |
| BspT104I | TTCGAA | 1 cut(s) | 381 |
| BspT107I | GGYRCC | 1 cut(s) | 2692 |
| BspTNI | GGTCTC | 2 cut(s) | 1079, 2856 |
| BsrBI | CCGCTC | 1 cut(s) | 251 |
| BsrDI | GCAATG | 2 cut(s) | 1406, 2048 |
| BsrFI | RCCGGY | 1 cut(s) | 179 |
| BsrI | ACTGG | 6 cut(s) | 359, 941, 1128, 1655, 2299, 2696 |
| BssAI | RCCGGY | 1 cut(s) | 179 |
| BssECI | CCNNGG | 3 cut(s) | 393, 1256, 1268 |
| BssNI | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| BssSI | CACGAG | 1 cut(s) | 2606 |
| BssT1I | CCWWGG | 2 cut(s) | 393, 1268 |
| Bst2BI | CACGAG | 1 cut(s) | 2606 |
| Bst2UI | CCWGG | 3 cut(s) | 1257, 1362, 2406 |
| Bst4CI | ACNGT | 8 cut(s) | 371, 952, 1496, 1726, 1818, 2147, 2179, 2922 |
| Bst6I | CTCTTC | 2 cut(s) | 2193, 2799 |
| BstACI | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1901, 2502 |
| BstBAI | YACGTR | 2 cut(s) | 464, 2614 |
| BstBI | TTCGAA | 1 cut(s) | 381 |
| BstC8I | GCNNGC | 5 cut(s) | 121, 978, 1046, 1935, 2986 |
| BstDSI | CCRYGG | 1 cut(s) | 393 |
| BstFNI | CGCG | 2 cut(s) | 151, 2811 |
| BstH2I | RGCGCY | 1 cut(s) | 1525 |
| BstHHI | GCGC | 2 cut(s) | 1524, 2813 |
| BstMAI | GTCTC | 7 cut(s) | 199, 1079, 1376, 1485, 2429, 2807, 2856 |
| BstMCI | CGRYCG | 2 cut(s) | 94, 1753 |
| BstNI | CCWGG | 3 cut(s) | 1257, 1362, 2406 |
| BstNSI | RCATGY | 3 cut(s) | 980, 1937, 2988 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 80 |
| BstSCI | CCNGG | 5 cut(s) | 1255, 1360, 1628, 2385, 2404 |
| BstSLI | GKGCMC | 1 cut(s) | 2613 |
| BstUI | CGCG | 2 cut(s) | 151, 2811 |
| BstV1I | GCAGC | 7 cut(s) | 628, 1282, 1703, 1977, 2230, 2299, 2572 |
| BstV2I | GAAGAC | 2 cut(s) | 946, 1109 |
| BstX2I | RGATCY | 4 cut(s) | 2536, 2789, 2912, 2941 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 400 |
| BstYI | RGATCY | 4 cut(s) | 2536, 2789, 2912, 2941 |
| BstZI | CGGCCG | 1 cut(s) | 91 |
| Bsu15I | ATCGAT | 1 cut(s) | 2499 |
| BsuI | GTATCC | 2 cut(s) | 537, 2167 |
| BsuRI | GGCC | 6 cut(s) | 93, 183, 333, 755, 2385, 2452 |
| BsuTUI | ATCGAT | 1 cut(s) | 2499 |
| BtgI | CCRYGG | 1 cut(s) | 393 |
| BtrI | CACGTC | 2 cut(s) | 2367, 2801 |
| BtsIMutI | CAGTG | 5 cut(s) | 302, 1059, 1662, 2072, 2746 |
| BveI | ACCTGC | 4 cut(s) | 1612, 2538, 2652, 2822 |
| Cac8I | GCNNGC | 5 cut(s) | 121, 978, 1046, 1935, 2986 |
| CaiI | CAGNNNCTG | 4 cut(s) | 791, 1901, 2183, 2765 |
| CciI | TCATGA | 1 cut(s) | 2140 |
| CfoI | GCGC | 2 cut(s) | 1524, 2813 |
| Cfr10I | RCCGGY | 1 cut(s) | 179 |
| Cfr13I | GGNCC | 4 cut(s) | 182, 754, 1253, 1282 |
| ClaI | ATCGAT | 1 cut(s) | 2499 |
| CseI | GACGC | 3 cut(s) | 149, 254, 812 |
| Csp6I | GTAC | 5 cut(s) | 453, 461, 866, 1196, 2918 |
| CviQI | GTAC | 5 cut(s) | 453, 461, 866, 1196, 2918 |
| EaeI | YGGCCR | 2 cut(s) | 91, 331 |
| EagI | CGGCCG | 1 cut(s) | 91 |
| Eam1104I | CTCTTC | 2 cut(s) | 2193, 2799 |
| EarI | CTCTTC | 2 cut(s) | 2193, 2799 |
| EciI | GGCGGA | 2 cut(s) | 25, 106 |
| Ecl136II | GAGCTC | 2 cut(s) | 486, 2251 |
| EclXI | CGGCCG | 1 cut(s) | 91 |
| Eco130I | CCWWGG | 2 cut(s) | 393, 1268 |
| Eco24I | GRGCYC | 2 cut(s) | 488, 2253 |
| Eco31I | GGTCTC | 2 cut(s) | 1079, 2856 |
| Eco32I | GATATC | 3 cut(s) | 1182, 2155, 2757 |
| Eco47I | GGWCC | 2 cut(s) | 1253, 1282 |
| Eco52I | CGGCCG | 1 cut(s) | 91 |
| Eco53kI | GAGCTC | 2 cut(s) | 486, 2251 |
| Eco57I | CTGAAG | 2 cut(s) | 326, 1721 |
| Eco72I | CACGTG | 1 cut(s) | 2614 |
| Eco88I | CYCGRG | 1 cut(s) | 319 |
| EcoICRI | GAGCTC | 2 cut(s) | 486, 2251 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1282 |
| EcoRI | GAATTC | 1 cut(s) | 2219 |
| EcoRII | CCWGG | 3 cut(s) | 1255, 1360, 2404 |
| EcoRV | GATATC | 3 cut(s) | 1182, 2155, 2757 |
| EcoT14I | CCWWGG | 2 cut(s) | 393, 1268 |
| EcoT22I | ATGCAT | 4 cut(s) | 988, 1935, 2498, 2684 |
| EcoT38I | GRGCYC | 2 cut(s) | 488, 2253 |
| ErhI | CCWWGG | 2 cut(s) | 393, 1268 |
| Esp3I | CGTCTC | 2 cut(s) | 199, 2807 |
| FaqI | GGGAC | 3 cut(s) | 1295, 2353, 2575 |
| FauI | CCCGC | 2 cut(s) | 128, 193 |
| FbaI | TGATCA | 3 cut(s) | 675, 1240, 2616 |
| FriOI | GRGCYC | 2 cut(s) | 488, 2253 |
| FspBI | CTAG | 7 cut(s) | 863, 1224, 1424, 1446, 1974, 2862, 2981 |
| GlaI | GCGC | 2 cut(s) | 1523, 2812 |
| GsaI | CCCAGC | 1 cut(s) | 1013 |
| HaeII | RGCGCY | 1 cut(s) | 1525 |
| HaeIII | GGCC | 6 cut(s) | 93, 183, 333, 755, 2385, 2452 |
| HapII | CCGG | 5 cut(s) | 65, 180, 1383, 1629, 2386 |
| HgaI | GACGC | 3 cut(s) | 149, 254, 812 |
| HhaI | GCGC | 2 cut(s) | 1524, 2813 |
| Hin1I | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| Hin6I | GCGC | 2 cut(s) | 1522, 2811 |
| HinP1I | GCGC | 2 cut(s) | 1522, 2811 |
| HincII | GTYRAC | 1 cut(s) | 2117 |
| HindII | GTYRAC | 1 cut(s) | 2117 |
| HindIII | AAGCTT | 1 cut(s) | 2343 |
| HpaII | CCGG | 5 cut(s) | 65, 180, 1383, 1629, 2386 |
| HphI | GGTGA | 7 cut(s) | 54, 779, 1350, 1366, 1409, 2591, 2940 |
| Hpy166II | GTNNAC | 3 cut(s) | 453, 2117, 2611 |
| Hpy8I | GTNNAC | 3 cut(s) | 453, 2117, 2611 |
| Hpy99I | CGWCG | 4 cut(s) | 143, 212, 248, 1947 |
| HpyAV | CCTTC | 7 cut(s) | 415, 917, 1295, 1430, 1599, 2463, 2955 |
| HpyCH4III | ACNGT | 8 cut(s) | 371, 952, 1496, 1726, 1818, 2147, 2179, 2922 |
| HpyCH4IV | ACGT | 7 cut(s) | 77, 463, 1945, 2366, 2613, 2800, 2959 |
| HpySE526I | ACGT | 7 cut(s) | 77, 463, 1945, 2366, 2613, 2800, 2959 |
| Hsp92I | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| HspAI | GCGC | 2 cut(s) | 1522, 2811 |
| Ksp22I | TGATCA | 3 cut(s) | 675, 1240, 2616 |
| LguI | GCTCTTC | 1 cut(s) | 2193 |
| LmnI | GCTCC | 4 cut(s) | 138, 206, 793, 2300 |
| Lsp1109I | GCAGC | 7 cut(s) | 628, 1282, 1703, 1977, 2230, 2299, 2572 |
| LweI | GCATC | 8 cut(s) | 574, 1384, 1650, 2055, 2483, 2505, 2572, 2860 |
| MaeI | CTAG | 7 cut(s) | 863, 1224, 1424, 1446, 1974, 2862, 2981 |
| MaeII | ACGT | 7 cut(s) | 77, 463, 1945, 2366, 2613, 2800, 2959 |
| MaeIII | GTNAC | 7 cut(s) | 82, 490, 698, 742, 1055, 2179, 2976 |
| MbiI | CCGCTC | 1 cut(s) | 251 |
| MboII | GAAGA | 8 cut(s) | 319, 553, 946, 1005, 1109, 2180, 2786, 2799 |
| MflI | RGATCY | 4 cut(s) | 2536, 2789, 2912, 2941 |
| MhlI | GDGCHC | 7 cut(s) | 488, 1679, 1890, 1917, 2253, 2485, 2613 |
| MlyI | GAGTC | 8 cut(s) | 50, 77, 206, 311, 1882, 2000, 2666, 2756 |
| MmeI | TCCRAC | 5 cut(s) | 24, 942, 1009, 1567, 2685 |
| Mph1103I | ATGCAT | 4 cut(s) | 988, 1935, 2498, 2684 |
| MseI | TTAA | 7 cut(s) | 1340, 1347, 1506, 1614, 2061, 2085, 2340 |
| MslI | CAYNNNNRTG | 2 cut(s) | 981, 1881 |
| MspI | CCGG | 5 cut(s) | 65, 180, 1383, 1629, 2386 |
| MspR9I | CCNGG | 5 cut(s) | 1257, 1362, 1630, 2387, 2406 |
| Mva1269I | GAATGC | 3 cut(s) | 558, 1561, 1701 |
| MvaI | CCWGG | 3 cut(s) | 1257, 1362, 2406 |
| MvnI | CGCG | 2 cut(s) | 151, 2811 |
| NciI | CCSGG | 2 cut(s) | 1630, 2387 |
| NcoI | CCATGG | 1 cut(s) | 393 |
| NlaIV | GGNNCC | 5 cut(s) | 6, 184, 202, 1283, 2694 |
| NmeAIII | GCCGAG | 3 cut(s) | 69, 119, 232 |
| NmuCI | GTSAC | 2 cut(s) | 82, 1055 |
| NsiI | ATGCAT | 4 cut(s) | 988, 1935, 2498, 2684 |
| NspI | RCATGY | 3 cut(s) | 980, 1937, 2988 |
| NspV | TTCGAA | 1 cut(s) | 381 |
| PaeI | GCATGC | 3 cut(s) | 980, 1937, 2988 |
| PaeR7I | CTCGAG | 1 cut(s) | 319 |
| PagI | TCATGA | 1 cut(s) | 2140 |
| PaqCI | CACCTGC | 1 cut(s) | 2822 |
| PciSI | GCTCTTC | 1 cut(s) | 2193 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 267 |
| PctI | GAATGC | 3 cut(s) | 558, 1561, 1701 |
| PfeI | GAWTC | 7 cut(s) | 794, 1414, 1898, 2096, 2390, 2543, 2572 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 340 |
| PfoI | TCCNGGA | 1 cut(s) | 1628 |
| Ple19I | CGATCG | 1 cut(s) | 1753 |
| PleI | GAGTC | 8 cut(s) | 50, 76, 206, 311, 1881, 2000, 2665, 2756 |
| PmaCI | CACGTG | 1 cut(s) | 2614 |
| PmlI | CACGTG | 1 cut(s) | 2614 |
| PpsI | GAGTC | 8 cut(s) | 50, 76, 206, 311, 1881, 2000, 2665, 2756 |
| Ppu21I | YACGTR | 2 cut(s) | 464, 2614 |
| PpuMI | RGGWCCY | 1 cut(s) | 1282 |
| PshAI | GACNNNNGTC | 1 cut(s) | 80 |
| PshBI | ATTAAT | 1 cut(s) | 1340 |
| Psp124BI | GAGCTC | 2 cut(s) | 488, 2253 |
| Psp5II | RGGWCCY | 1 cut(s) | 1282 |
| Psp6I | CCWGG | 3 cut(s) | 1255, 1360, 2404 |
| PspCI | CACGTG | 1 cut(s) | 2614 |
| PspFI | CCCAGC | 1 cut(s) | 1009 |
| PspGI | CCWGG | 3 cut(s) | 1255, 1360, 2404 |
| PspN4I | GGNNCC | 5 cut(s) | 6, 184, 202, 1283, 2694 |
| PspPI | GGNCC | 4 cut(s) | 182, 754, 1253, 1282 |
| PspPPI | RGGWCCY | 1 cut(s) | 1282 |
| PspXI | VCTCGAGB | 1 cut(s) | 319 |
| PstNI | CAGNNNCTG | 4 cut(s) | 791, 1901, 2183, 2765 |
| PsuI | RGATCY | 4 cut(s) | 2536, 2789, 2912, 2941 |
| PvuI | CGATCG | 1 cut(s) | 1753 |
| RsaI | GTAC | 5 cut(s) | 454, 462, 867, 1197, 2919 |
| RsaNI | GTAC | 5 cut(s) | 453, 461, 866, 1196, 2918 |
| RseI | CAYNNNNRTG | 2 cut(s) | 981, 1881 |
| SacI | GAGCTC | 2 cut(s) | 488, 2253 |
| SapI | GCTCTTC | 1 cut(s) | 2193 |
| SaqAI | TTAA | 7 cut(s) | 1340, 1347, 1506, 1614, 2061, 2085, 2340 |
| Sau96I | GGNCC | 4 cut(s) | 182, 754, 1253, 1282 |
| SchI | GAGTC | 8 cut(s) | 50, 77, 206, 311, 1882, 2000, 2666, 2756 |
| ScrFI | CCNGG | 5 cut(s) | 1257, 1362, 1630, 2387, 2406 |
| SduI | GDGCHC | 7 cut(s) | 488, 1679, 1890, 1917, 2253, 2485, 2613 |
| SfaNI | GCATC | 8 cut(s) | 574, 1384, 1650, 2055, 2483, 2505, 2572, 2860 |
| Sfr274I | CTCGAG | 1 cut(s) | 319 |
| SfuI | TTCGAA | 1 cut(s) | 381 |
| SinI | GGWCC | 2 cut(s) | 1253, 1282 |
| SlaI | CTCGAG | 1 cut(s) | 319 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 981, 1881 |
| SmlI | CTYRAG | 2 cut(s) | 319, 2271 |
| SmoI | CTYRAG | 2 cut(s) | 319, 2271 |
| SphI | GCATGC | 3 cut(s) | 980, 1937, 2988 |
| SspI | AATATT | 2 cut(s) | 943, 1344 |
| SspMI | CTAG | 7 cut(s) | 863, 1224, 1424, 1446, 1974, 2862, 2981 |
| SstI | GAGCTC | 2 cut(s) | 488, 2253 |
| StyD4I | CCNGG | 5 cut(s) | 1255, 1360, 1628, 2385, 2404 |
| StyI | CCWWGG | 2 cut(s) | 393, 1268 |
| TaaI | ACNGT | 8 cut(s) | 371, 952, 1496, 1726, 1818, 2147, 2179, 2922 |
| TaiI | ACGT | 7 cut(s) | 80, 466, 1948, 2369, 2616, 2803, 2962 |
| TatI | WGTACW | 3 cut(s) | 452, 865, 2917 |
| TauI | GCSGC | 4 cut(s) | 154, 251, 333, 739 |
| TfiI | GAWTC | 7 cut(s) | 794, 1414, 1898, 2096, 2390, 2543, 2572 |
| Tru1I | TTAA | 7 cut(s) | 1340, 1347, 1506, 1614, 2061, 2085, 2340 |
| Tru9I | TTAA | 7 cut(s) | 1340, 1347, 1506, 1614, 2061, 2085, 2340 |
| TscAI | CASTG | 5 cut(s) | 309, 1066, 1662, 2079, 2746 |
| TseFI | GTSAC | 2 cut(s) | 82, 1055 |
| TseI | GCWGC | 7 cut(s) | 641, 1295, 1716, 1965, 2243, 2312, 2585 |
| Tsp45I | GTSAC | 2 cut(s) | 82, 1055 |
| TspGWI | ACGGA | 1 cut(s) | 181 |
| TspRI | CASTG | 5 cut(s) | 309, 1066, 1662, 2079, 2746 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 340 |
| VneI | GTGCAC | 1 cut(s) | 2609 |
| VpaK11BI | GGWCC | 2 cut(s) | 1253, 1282 |
| VspI | ATTAAT | 1 cut(s) | 1340 |
| XapI | RAATTY | 7 cut(s) | 276, 718, 889, 1002, 1093, 1145, 2219 |
| XbaI | TCTAGA | 2 cut(s) | 1423, 1973 |
| XceI | RCATGY | 3 cut(s) | 980, 1937, 2988 |
| XhoI | CTCGAG | 1 cut(s) | 319 |
| XspI | CTAG | 7 cut(s) | 863, 1224, 1424, 1446, 1974, 2862, 2981 |
| ZraI | GACGTC | 1 cut(s) | 78 |
| Zsp2I | ATGCAT | 4 cut(s) | 988, 1935, 2498, 2684 |