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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G57570 AT3G57570 |
| fragaria_vesca | FvH4_2g02670 FvH4_2g02670 FvH4_2g02670 FvH4_2g02670 FvH4_2g02670 FvH4_2g02670 FvH4_7g07942 FvH4_7g07943 |
| malus_domestica | MD05G1111300.v1.1 |
| prunus_persica | Prupe.8G156400_v2.0.a1 Prupe.8G156400_v2.0.a1 |
| pyrus_communis | pycom05g10710 |
| rosa_chinensis | RchiOBHm_Chr6g0247071 RchiOBHm_Chr7g0177161 |
| rosa_laevigata | RLG00000015224 |
| rosa_multiflora | Rmu_sc0002476.1_g000020 Rmu_sc0004577.1_g000007 Rmu_sc0005310.1_g000018 Rmu_sc0027214.1_g000001 |
| rosa_roxburghii | Rroxscaffold_7G00214360 |
| rosa_rugosa | Rorug05G0522000.1 |
| rosa_samantha | Rh6AG037100 Rh6BG031800 Rh6CG033000 Rh6CG033100 Rh6DG030800 Rh7AG003700 Rh7CG003400 |
| rosa_wichuraiana | Rw6G003180 Rw7G000300 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 3125 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1316 |
| AatII | GACGTC | 1 cut(s) | 80 |
| Acc36I | ACCTGC | 3 cut(s) | 1121, 1741, 3125 |
| AccB1I | GGYRCC | 1 cut(s) | 2854 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 340 |
| AccBSI | CCGCTC | 2 cut(s) | 251, 2348 |
| AccII | CGCG | 1 cut(s) | 2973 |
| AclWI | GGATC | 9 cut(s) | 846, 1107, 1903, 2693, 3084, 3121, 3160, 3210, 3239 |
| AcoI | YGGCCR | 3 cut(s) | 91, 331, 737 |
| AcsI | RAATTY | 3 cut(s) | 1070, 1223, 2381 |
| AcuI | CTGAAG | 1 cut(s) | 1851 |
| AcyI | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| AdeI | CACNNNGTG | 1 cut(s) | 555 |
| AfaI | GTAC | 9 cut(s) | 454, 462, 867, 1275, 2774, 2808, 3016, 3041, 3222 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 150, 340, 509, 1201, 2625, 2626, 2884 |
| AflII | CTTAAG | 1 cut(s) | 932 |
| AjiI | CACGTC | 2 cut(s) | 2529, 2963 |
| AjnI | CCWGG | 4 cut(s) | 333, 1357, 2566, 2619 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2440, 2472 |
| AleI | CACNNNNGTG | 1 cut(s) | 2742 |
| Alw21I | GWGCWC | 5 cut(s) | 488, 1809, 2052, 2079, 2415 |
| Alw26I | GTCTC | 9 cut(s) | 199, 1157, 1243, 1373, 1482, 1881, 2591, 2969, 3094 |
| AlwI | GGATC | 9 cut(s) | 846, 1107, 1903, 2693, 3084, 3121, 3160, 3210, 3239 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 791, 2063, 2927 |
| AoxI | GGCC | 7 cut(s) | 91, 181, 331, 737, 753, 2545, 2612 |
| ApoI | RAATTY | 3 cut(s) | 1070, 1223, 2381 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1979, 2011 |
| AseI | ATTAAT | 2 cut(s) | 1337, 1709 |
| AspLEI | GCGC | 2 cut(s) | 1521, 2975 |
| AspS9I | GGNCC | 2 cut(s) | 182, 754 |
| AsuC2I | CCSGG | 2 cut(s) | 1759, 2549 |
| AsuHPI | GGTGA | 8 cut(s) | 54, 163, 779, 1347, 1363, 1406, 2995, 3243 |
| AsuII | TTCGAA | 1 cut(s) | 381 |
| AsuNHI | GCTAGC | 1 cut(s) | 3283 |
| BanI | GGYRCC | 1 cut(s) | 2854 |
| BanII | GRGCYC | 2 cut(s) | 488, 2415 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1266, 1298 |
| BauI | CACGAG | 2 cut(s) | 944, 2845 |
| BbsI | GAAGAC | 1 cut(s) | 1014 |
| Bbv12I | GWGCWC | 5 cut(s) | 488, 1809, 2052, 2079, 2415 |
| BbvCI | CCTCAGC | 2 cut(s) | 978, 1868 |
| BceAI | ACGGC | 4 cut(s) | 128, 343, 617, 2960 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 371, 405, 2836, 2870 |
| BciT130I | CCWGG | 4 cut(s) | 335, 1359, 2568, 2621 |
| BciVI | GTATCC | 2 cut(s) | 537, 2329 |
| BclI | TGATCA | 2 cut(s) | 675, 2778 |
| BcnI | CCSGG | 2 cut(s) | 1759, 2549 |
| BcoDI | GTCTC | 9 cut(s) | 199, 1157, 1243, 1373, 1482, 1881, 2591, 2969, 3094 |
| BfaI | CTAG | 6 cut(s) | 863, 1421, 1443, 2136, 3165, 3284 |
| BfoI | RGCGCY | 1 cut(s) | 1522 |
| BfrI | CTTAAG | 1 cut(s) | 932 |
| BfuAI | ACCTGC | 3 cut(s) | 1121, 1741, 3125 |
| BfuI | GTATCC | 2 cut(s) | 537, 2329 |
| BglII | AGATCT | 1 cut(s) | 2951 |
| BlpI | GCTNAGC | 2 cut(s) | 2409, 2688 |
| Bme1390I | CCNGG | 6 cut(s) | 335, 1359, 1759, 2549, 2568, 2621 |
| BmgBI | CACGTC | 2 cut(s) | 2529, 2963 |
| BmgT120I | GGNCC | 2 cut(s) | 182, 754 |
| BmiI | GGNNCC | 4 cut(s) | 6, 184, 202, 2856 |
| BmrFI | CCNGG | 6 cut(s) | 335, 1359, 1759, 2549, 2568, 2621 |
| BmrI | ACTGGG | 2 cut(s) | 1211, 2455 |
| BmsI | GCATC | 8 cut(s) | 574, 1381, 1700, 1780, 2217, 2645, 2667, 3163 |
| BmtI | GCTAGC | 1 cut(s) | 3287 |
| BmuI | ACTGGG | 2 cut(s) | 1211, 2455 |
| BoxI | GACNNNNGTC | 1 cut(s) | 80 |
| BpiI | GAAGAC | 1 cut(s) | 1014 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 971, 1003, 1246, 1278 |
| Bpu10I | CCTNAGC | 3 cut(s) | 978, 1868, 1922 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 2409, 2688 |
| Bpu14I | TTCGAA | 1 cut(s) | 381 |
| BpuEI | CTTGAG | 2 cut(s) | 2418, 3003 |
| BpuMI | CCSGG | 2 cut(s) | 1759, 2549 |
| Bsa29I | ATCGAT | 1 cut(s) | 2661 |
| BsaAI | YACGTR | 2 cut(s) | 464, 2776 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1774 |
| BsaHI | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| BsaI | GGTCTC | 1 cut(s) | 1157 |
| BsaJI | CCNNGG | 4 cut(s) | 393, 1758, 2619, 3072 |
| BsaWI | WCCGGW | 2 cut(s) | 64, 1379 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 858, 888 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 150, 340, 509, 1201, 2625, 2626, 2884 |
| Bse118I | RCCGGY | 2 cut(s) | 179, 739 |
| Bse1I | ACTGG | 8 cut(s) | 359, 1009, 1206, 1695, 1785, 2286, 2461, 2858 |
| Bse3DI | GCAATG | 2 cut(s) | 1403, 2210 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1774 |
| BseBI | CCWGG | 4 cut(s) | 335, 1359, 2568, 2621 |
| BseCI | ATCGAT | 1 cut(s) | 2661 |
| BseDI | CCNNGG | 4 cut(s) | 393, 1758, 2619, 3072 |
| BseJI | GATNNNNATC | 1 cut(s) | 1774 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 150, 340, 509, 1201, 2625, 2626, 2884 |
| BseMI | GCAATG | 2 cut(s) | 1403, 2210 |
| BseNI | ACTGG | 8 cut(s) | 359, 1009, 1206, 1695, 1785, 2286, 2461, 2858 |
| BseRI | GAGGAG | 4 cut(s) | 94, 150, 218, 1561 |
| BseX3I | CGGCCG | 2 cut(s) | 91, 737 |
| BseYI | CCCAGC | 2 cut(s) | 1077, 1614 |
| BsgI | GTGCAG | 1 cut(s) | 566 |
| Bsh1236I | CGCG | 1 cut(s) | 2973 |
| Bsh1285I | CGRYCG | 3 cut(s) | 94, 740, 1915 |
| BshFI | GGCC | 7 cut(s) | 93, 183, 333, 739, 755, 2547, 2614 |
| BshNI | GGYRCC | 1 cut(s) | 2854 |
| BshVI | ATCGAT | 1 cut(s) | 2661 |
| BsiEI | CGRYCG | 3 cut(s) | 94, 740, 1915 |
| BsiHKAI | GWGCWC | 5 cut(s) | 488, 1809, 2052, 2079, 2415 |
| BsiSI | CCGG | 6 cut(s) | 65, 180, 740, 1380, 1758, 2548 |
| BslFI | GGGAC | 2 cut(s) | 2515, 2737 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 150, 340, 509, 1201, 2625, 2626, 2884 |
| BsmAI | GTCTC | 9 cut(s) | 199, 1157, 1243, 1373, 1482, 1881, 2591, 2969, 3094 |
| BsmBI | CGTCTC | 2 cut(s) | 199, 2969 |
| BsmFI | GGGAC | 2 cut(s) | 2515, 2737 |
| BsmI | GAATGC | 3 cut(s) | 558, 1558, 1831 |
| BsnI | GGCC | 7 cut(s) | 93, 183, 333, 739, 755, 2547, 2614 |
| Bso31I | GGTCTC | 1 cut(s) | 1157 |
| Bsp119I | TTCGAA | 1 cut(s) | 381 |
| Bsp1286I | GDGCHC | 5 cut(s) | 488, 1809, 2052, 2079, 2415 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 2409, 2688 |
| Bsp19I | CCATGG | 2 cut(s) | 393, 3072 |
| BspANI | GGCC | 7 cut(s) | 93, 183, 333, 739, 755, 2547, 2614 |
| BspDI | ATCGAT | 1 cut(s) | 2661 |
| BspFNI | CGCG | 1 cut(s) | 2973 |
| BspHI | TCATGA | 1 cut(s) | 2302 |
| BspLI | GGNNCC | 4 cut(s) | 6, 184, 202, 2856 |
| BspMI | ACCTGC | 3 cut(s) | 1121, 1741, 3125 |
| BspOI | GCTAGC | 1 cut(s) | 3287 |
| BspPI | GGATC | 9 cut(s) | 846, 1107, 1903, 2693, 3084, 3121, 3160, 3210, 3239 |
| BspQI | GCTCTTC | 2 cut(s) | 2355, 3108 |
| BspT104I | TTCGAA | 1 cut(s) | 381 |
| BspT107I | GGYRCC | 1 cut(s) | 2854 |
| BspTI | CTTAAG | 1 cut(s) | 932 |
| BspTNI | GGTCTC | 1 cut(s) | 1157 |
| BsrBI | CCGCTC | 2 cut(s) | 251, 2348 |
| BsrDI | GCAATG | 2 cut(s) | 1403, 2210 |
| BsrFI | RCCGGY | 2 cut(s) | 179, 739 |
| BsrI | ACTGG | 8 cut(s) | 359, 1009, 1206, 1695, 1785, 2286, 2461, 2858 |
| BssAI | RCCGGY | 2 cut(s) | 179, 739 |
| BssECI | CCNNGG | 4 cut(s) | 393, 1758, 2619, 3072 |
| BssNI | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| BssSI | CACGAG | 2 cut(s) | 944, 2845 |
| BssT1I | CCWWGG | 2 cut(s) | 393, 3072 |
| Bst2BI | CACGAG | 2 cut(s) | 944, 2845 |
| Bst2UI | CCWGG | 4 cut(s) | 335, 1359, 2568, 2621 |
| Bst4CI | ACNGT | 9 cut(s) | 371, 1020, 1122, 1493, 1856, 1980, 2309, 2341, 3225 |
| Bst6I | CTCTTC | 4 cut(s) | 114, 2355, 2961, 3108 |
| BstACI | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| BstAFI | CTTAAG | 1 cut(s) | 932 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 2063, 2664 |
| BstBAI | YACGTR | 2 cut(s) | 464, 2776 |
| BstBI | TTCGAA | 1 cut(s) | 381 |
| BstC8I | GCNNGC | 7 cut(s) | 985, 1046, 1114, 2097, 2908, 3285, 3289 |
| BstDSI | CCRYGG | 2 cut(s) | 393, 3072 |
| BstFNI | CGCG | 1 cut(s) | 2973 |
| BstH2I | RGCGCY | 1 cut(s) | 1522 |
| BstHHI | GCGC | 2 cut(s) | 1521, 2975 |
| BstMAI | GTCTC | 9 cut(s) | 199, 1157, 1243, 1373, 1482, 1881, 2591, 2969, 3094 |
| BstMCI | CGRYCG | 3 cut(s) | 94, 740, 1915 |
| BstNI | CCWGG | 4 cut(s) | 335, 1359, 2568, 2621 |
| BstNSI | RCATGY | 5 cut(s) | 911, 1048, 2099, 3015, 3291 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 80 |
| BstSCI | CCNGG | 6 cut(s) | 333, 1357, 1757, 2547, 2566, 2619 |
| BstUI | CGCG | 1 cut(s) | 2973 |
| BstV2I | GAAGAC | 1 cut(s) | 1014 |
| BstX2I | RGATCY | 6 cut(s) | 2698, 2951, 3076, 3152, 3215, 3244 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 400, 919 |
| BstYI | RGATCY | 6 cut(s) | 2698, 2951, 3076, 3152, 3215, 3244 |
| BstZI | CGGCCG | 2 cut(s) | 91, 737 |
| Bsu15I | ATCGAT | 1 cut(s) | 2661 |
| BsuI | GTATCC | 2 cut(s) | 537, 2329 |
| BsuRI | GGCC | 7 cut(s) | 93, 183, 333, 739, 755, 2547, 2614 |
| BsuTUI | ATCGAT | 1 cut(s) | 2661 |
| BtgI | CCRYGG | 2 cut(s) | 393, 3072 |
| BtrI | CACGTC | 2 cut(s) | 2529, 2963 |
| BtsI | GCAGTG | 1 cut(s) | 3042 |
| BtsIMutI | CAGTG | 4 cut(s) | 1137, 1502, 1792, 3042 |
| BveI | ACCTGC | 3 cut(s) | 1121, 1741, 3125 |
| Cac8I | GCNNGC | 7 cut(s) | 985, 1046, 1114, 2097, 2908, 3285, 3289 |
| CaiI | CAGNNNCTG | 3 cut(s) | 791, 2063, 2927 |
| CciI | TCATGA | 1 cut(s) | 2302 |
| CfoI | GCGC | 2 cut(s) | 1521, 2975 |
| Cfr10I | RCCGGY | 2 cut(s) | 179, 739 |
| Cfr13I | GGNCC | 2 cut(s) | 182, 754 |
| ClaI | ATCGAT | 1 cut(s) | 2661 |
| CseI | GACGC | 3 cut(s) | 149, 254, 812 |
| Csp6I | GTAC | 9 cut(s) | 453, 461, 866, 1274, 2773, 2807, 3015, 3040, 3221 |
| CviQI | GTAC | 9 cut(s) | 453, 461, 866, 1274, 2773, 2807, 3015, 3040, 3221 |
| DraI | TTTAAA | 1 cut(s) | 723 |
| DraIII | CACNNNGTG | 1 cut(s) | 555 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1316 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1316 |
| EaeI | YGGCCR | 3 cut(s) | 91, 331, 737 |
| EagI | CGGCCG | 2 cut(s) | 91, 737 |
| Eam1104I | CTCTTC | 4 cut(s) | 114, 2355, 2961, 3108 |
| EarI | CTCTTC | 4 cut(s) | 114, 2355, 2961, 3108 |
| EciI | GGCGGA | 1 cut(s) | 25 |
| Ecl136II | GAGCTC | 2 cut(s) | 486, 2413 |
| EclXI | CGGCCG | 2 cut(s) | 91, 737 |
| Eco130I | CCWWGG | 2 cut(s) | 393, 3072 |
| Eco24I | GRGCYC | 2 cut(s) | 488, 2415 |
| Eco31I | GGTCTC | 1 cut(s) | 1157 |
| Eco32I | GATATC | 3 cut(s) | 1260, 2317, 2919 |
| Eco52I | CGGCCG | 2 cut(s) | 91, 737 |
| Eco53kI | GAGCTC | 2 cut(s) | 486, 2413 |
| Eco57I | CTGAAG | 1 cut(s) | 1851 |
| EcoICRI | GAGCTC | 2 cut(s) | 486, 2413 |
| EcoRI | GAATTC | 1 cut(s) | 2381 |
| EcoRII | CCWGG | 4 cut(s) | 333, 1357, 2566, 2619 |
| EcoRV | GATATC | 3 cut(s) | 1260, 2317, 2919 |
| EcoT14I | CCWWGG | 2 cut(s) | 393, 3072 |
| EcoT22I | ATGCAT | 4 cut(s) | 1056, 2097, 2660, 2885 |
| EcoT38I | GRGCYC | 2 cut(s) | 488, 2415 |
| ErhI | CCWWGG | 2 cut(s) | 393, 3072 |
| Esp3I | CGTCTC | 2 cut(s) | 199, 2969 |
| FaqI | GGGAC | 2 cut(s) | 2515, 2737 |
| FauI | CCCGC | 2 cut(s) | 128, 193 |
| FauNDI | CATATG | 1 cut(s) | 1584 |
| FbaI | TGATCA | 2 cut(s) | 675, 2778 |
| FriOI | GRGCYC | 2 cut(s) | 488, 2415 |
| FspBI | CTAG | 6 cut(s) | 863, 1421, 1443, 2136, 3165, 3284 |
| GlaI | GCGC | 2 cut(s) | 1520, 2974 |
| GsaI | CCCAGC | 2 cut(s) | 1081, 1618 |
| HaeII | RGCGCY | 1 cut(s) | 1522 |
| HaeIII | GGCC | 7 cut(s) | 93, 183, 333, 739, 755, 2547, 2614 |
| HapII | CCGG | 6 cut(s) | 65, 180, 740, 1380, 1758, 2548 |
| HgaI | GACGC | 3 cut(s) | 149, 254, 812 |
| HhaI | GCGC | 2 cut(s) | 1521, 2975 |
| Hin1I | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| Hin6I | GCGC | 2 cut(s) | 1519, 2973 |
| HinP1I | GCGC | 2 cut(s) | 1519, 2973 |
| HincII | GTYRAC | 1 cut(s) | 2279 |
| HindII | GTYRAC | 1 cut(s) | 2279 |
| HindIII | AAGCTT | 1 cut(s) | 2505 |
| HpaII | CCGG | 6 cut(s) | 65, 180, 740, 1380, 1758, 2548 |
| HphI | GGTGA | 8 cut(s) | 54, 163, 779, 1347, 1363, 1406, 2995, 3243 |
| Hpy166II | GTNNAC | 3 cut(s) | 453, 2279, 3040 |
| Hpy8I | GTNNAC | 3 cut(s) | 453, 2279, 3040 |
| Hpy99I | CGWCG | 6 cut(s) | 46, 143, 212, 248, 421, 2109 |
| HpyAV | CCTTC | 7 cut(s) | 985, 1205, 1427, 1728, 2625, 3115, 3258 |
| HpyCH4III | ACNGT | 9 cut(s) | 371, 1020, 1122, 1493, 1856, 1980, 2309, 2341, 3225 |
| HpyCH4IV | ACGT | 9 cut(s) | 77, 463, 969, 2107, 2528, 2775, 2805, 2962, 3262 |
| HpySE526I | ACGT | 9 cut(s) | 77, 463, 969, 2107, 2528, 2775, 2805, 2962, 3262 |
| Hsp92I | GRCGYC | 4 cut(s) | 77, 141, 246, 804 |
| HspAI | GCGC | 2 cut(s) | 1519, 2973 |
| Ksp22I | TGATCA | 2 cut(s) | 675, 2778 |
| LguI | GCTCTTC | 2 cut(s) | 2355, 3108 |
| LmnI | GCTCC | 5 cut(s) | 206, 793, 885, 922, 1516 |
| LweI | GCATC | 8 cut(s) | 574, 1381, 1700, 1780, 2217, 2645, 2667, 3163 |
| MaeI | CTAG | 6 cut(s) | 863, 1421, 1443, 2136, 3165, 3284 |
| MaeII | ACGT | 9 cut(s) | 77, 463, 969, 2107, 2528, 2775, 2805, 2962, 3262 |
| MaeIII | GTNAC | 7 cut(s) | 82, 490, 698, 742, 965, 1133, 2341 |
| MbiI | CCGCTC | 2 cut(s) | 251, 2348 |
| MfeI | CAATTG | 1 cut(s) | 573 |
| MflI | RGATCY | 6 cut(s) | 2698, 2951, 3076, 3152, 3215, 3244 |
| MhlI | GDGCHC | 5 cut(s) | 488, 1809, 2052, 2079, 2415 |
| MlyI | GAGTC | 8 cut(s) | 50, 77, 206, 311, 2044, 2162, 2828, 2918 |
| MmeI | TCCRAC | 6 cut(s) | 24, 1010, 1077, 1564, 2034, 2847 |
| Mph1103I | ATGCAT | 4 cut(s) | 1056, 2097, 2660, 2885 |
| MslI | CAYNNNNRTG | 5 cut(s) | 287, 1049, 1583, 2043, 2742 |
| MspA1I | CMGCKG | 2 cut(s) | 2236, 2742 |
| MspCI | CTTAAG | 1 cut(s) | 932 |
| MspI | CCGG | 6 cut(s) | 65, 180, 740, 1380, 1758, 2548 |
| MspR9I | CCNGG | 6 cut(s) | 335, 1359, 1759, 2549, 2568, 2621 |
| MunI | CAATTG | 1 cut(s) | 573 |
| Mva1269I | GAATGC | 3 cut(s) | 558, 1558, 1831 |
| MvaI | CCWGG | 4 cut(s) | 335, 1359, 2568, 2621 |
| MvnI | CGCG | 1 cut(s) | 2973 |
| NciI | CCSGG | 2 cut(s) | 1759, 2549 |
| NcoI | CCATGG | 2 cut(s) | 393, 3072 |
| NdeI | CATATG | 1 cut(s) | 1584 |
| NheI | GCTAGC | 1 cut(s) | 3283 |
| NlaIV | GGNNCC | 4 cut(s) | 6, 184, 202, 2856 |
| NmeAIII | GCCGAG | 3 cut(s) | 69, 119, 232 |
| NmuCI | GTSAC | 2 cut(s) | 82, 1133 |
| NsiI | ATGCAT | 4 cut(s) | 1056, 2097, 2660, 2885 |
| NspI | RCATGY | 5 cut(s) | 911, 1048, 2099, 3015, 3291 |
| NspV | TTCGAA | 1 cut(s) | 381 |
| OliI | CACNNNNGTG | 1 cut(s) | 2742 |
| PaeI | GCATGC | 3 cut(s) | 1048, 2099, 3291 |
| PagI | TCATGA | 1 cut(s) | 2302 |
| PaqCI | CACCTGC | 1 cut(s) | 3125 |
| PciSI | GCTCTTC | 2 cut(s) | 2355, 3108 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 267 |
| PctI | GAATGC | 3 cut(s) | 558, 1558, 1831 |
| PfeI | GAWTC | 6 cut(s) | 794, 1411, 1595, 2552, 2705, 2734 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 340 |
| Ple19I | CGATCG | 1 cut(s) | 1915 |
| PleI | GAGTC | 8 cut(s) | 50, 76, 206, 311, 2043, 2162, 2827, 2918 |
| PpsI | GAGTC | 8 cut(s) | 50, 76, 206, 311, 2043, 2162, 2827, 2918 |
| Ppu21I | YACGTR | 2 cut(s) | 464, 2776 |
| PshAI | GACNNNNGTC | 1 cut(s) | 80 |
| PshBI | ATTAAT | 2 cut(s) | 1337, 1709 |
| Psp124BI | GAGCTC | 2 cut(s) | 488, 2415 |
| Psp6I | CCWGG | 4 cut(s) | 333, 1357, 2566, 2619 |
| PspFI | CCCAGC | 2 cut(s) | 1077, 1614 |
| PspGI | CCWGG | 4 cut(s) | 333, 1357, 2566, 2619 |
| PspN4I | GGNNCC | 4 cut(s) | 6, 184, 202, 2856 |
| PspPI | GGNCC | 2 cut(s) | 182, 754 |
| PstNI | CAGNNNCTG | 3 cut(s) | 791, 2063, 2927 |
| PsuI | RGATCY | 6 cut(s) | 2698, 2951, 3076, 3152, 3215, 3244 |
| PvuI | CGATCG | 1 cut(s) | 1915 |
| RsaI | GTAC | 9 cut(s) | 454, 462, 867, 1275, 2774, 2808, 3016, 3041, 3222 |
| RsaNI | GTAC | 9 cut(s) | 453, 461, 866, 1274, 2773, 2807, 3015, 3040, 3221 |
| RseI | CAYNNNNRTG | 5 cut(s) | 287, 1049, 1583, 2043, 2742 |
| SacI | GAGCTC | 2 cut(s) | 488, 2415 |
| SapI | GCTCTTC | 2 cut(s) | 2355, 3108 |
| Sau96I | GGNCC | 2 cut(s) | 182, 754 |
| SchI | GAGTC | 8 cut(s) | 50, 77, 206, 311, 2044, 2162, 2828, 2918 |
| ScrFI | CCNGG | 6 cut(s) | 335, 1359, 1759, 2549, 2568, 2621 |
| SduI | GDGCHC | 5 cut(s) | 488, 1809, 2052, 2079, 2415 |
| SfaNI | GCATC | 8 cut(s) | 574, 1381, 1700, 1780, 2217, 2645, 2667, 3163 |
| SfuI | TTCGAA | 1 cut(s) | 381 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 287, 1049, 1583, 2043, 2742 |
| SmlI | CTYRAG | 3 cut(s) | 932, 2433, 3018 |
| SmoI | CTYRAG | 3 cut(s) | 932, 2433, 3018 |
| SphI | GCATGC | 3 cut(s) | 1048, 2099, 3291 |
| SspI | AATATT | 2 cut(s) | 1011, 1341 |
| SspMI | CTAG | 6 cut(s) | 863, 1421, 1443, 2136, 3165, 3284 |
| SstI | GAGCTC | 2 cut(s) | 488, 2415 |
| StyD4I | CCNGG | 6 cut(s) | 333, 1357, 1757, 2547, 2566, 2619 |
| StyI | CCWWGG | 2 cut(s) | 393, 3072 |
| TaaI | ACNGT | 9 cut(s) | 371, 1020, 1122, 1493, 1856, 1980, 2309, 2341, 3225 |
| TaiI | ACGT | 9 cut(s) | 80, 466, 972, 2110, 2531, 2778, 2808, 2965, 3265 |
| TatI | WGTACW | 3 cut(s) | 452, 865, 3014 |
| TauI | GCSGC | 4 cut(s) | 154, 251, 333, 739 |
| TfiI | GAWTC | 6 cut(s) | 794, 1411, 1595, 2552, 2705, 2734 |
| TscAI | CASTG | 4 cut(s) | 1144, 1502, 1792, 3042 |
| TseFI | GTSAC | 2 cut(s) | 82, 1133 |
| Tsp45I | GTSAC | 2 cut(s) | 82, 1133 |
| TspGWI | ACGGA | 2 cut(s) | 181, 3208 |
| TspRI | CASTG | 4 cut(s) | 1144, 1502, 1792, 3042 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 340 |
| Vha464I | CTTAAG | 1 cut(s) | 932 |
| VspI | ATTAAT | 2 cut(s) | 1337, 1709 |
| XapI | RAATTY | 3 cut(s) | 1070, 1223, 2381 |
| XbaI | TCTAGA | 2 cut(s) | 1420, 2135 |
| XceI | RCATGY | 5 cut(s) | 911, 1048, 2099, 3015, 3291 |
| XspI | CTAG | 6 cut(s) | 863, 1421, 1443, 2136, 3165, 3284 |
| ZraI | GACGTC | 1 cut(s) | 78 |
| Zsp2I | ATGCAT | 4 cut(s) | 1056, 2097, 2660, 2885 |