Rmu_sc0004647.1_g000014

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004647.1
OG0004352
Physical Location & Seq
Forward (+)
52432 .. 96917
44486 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 14763 bp
atgatgaccatgatggatcttgcattaaggattttgctaggggtgggcacggttcgggccggctcggttttgggcccaacccgttttcgaaccgtccatttcggttggcccaaatctcaaccggcagcccgattcattttcaaatgggccgatatgttcggttgggccgaaaatgctgttcggtccaggtttcacggttcatcttgggatctaaaatcacgtgagggagtgatgtgggcacatggtcacgcgcaactgaccccagatggcaataatgctaatccccccttcccgattttttccaaaataggcataatcgcatatgccccaaaacgacgtcactccgaccctccttgtcccaaagccccaatctcctacttccctccacccgcacagtccaactgccacccgacagtcctatgcctttcatttccttgcggcccacgtcagtccccatcacacgcgtcgacgttttattcgtccagggatttccggccggcgaagatgttggaggatcaggtggcgaacctcttgcagaggtacctggggaactacgtgaagggactcaataaagaggccctcaagatcagtgtctggcaaggtgatgtggagctaacaaatatgcagttgaagccagaggcactgaatgccttaaaactacctgtgaaggtcaaggcaggatttcttggatcagtgaaacttaaggttccatggagcaggcttggccaggatccagtgttagtttctttggatcgcatttttctattagctgagcctgaaacgcaagttgagggaagtagtgaggatgctgtacaggaaactaagaaaaaccgagtgcaggaaatggaaatgaagttgttggagaaggcgcaacaactgaagtcagatgtgaataaatcatggttgggatccctgatcagcacaataattggaaatttgaaactttcaatttcaaatattcacatcagatacgaggatcttgagagcaattttggtcatccgtttgcagcgggcataaccttggagagactttcagccatgacagttgatgatactggaaaagagacatttgttactgggggtgcactgaactgtattcagaagtctgtcgaacttgatcggcttgcactatatctggattctgatattgctccatggcatgttaacaagcaatgggaggatttgcttccatcagagtgggtccaggtttttaaatttggtactaaagatggtaaaccagcgaataaatcgaccaagaagcatacttatgtattggaacctgtttctggaagtgctaagtattcaaagctacgacaaaatgaatttgctgatagtggtcggcctttacataaagcagcagttaatctggatgatgtgactatctgtttgccaaaggacggatacagggatgtactgaagttagctgataactttgctgccttcaatcagcggttgaagtatgctcattatcgtccacatgtctctgtaaaatctgatcctaggtcttggtggaagtatgcttacagagttgtttctgaccaaatgaagaaggcaagtgggaaactgtcatgggagcaagttctgaagtatgcaagattacgtaaaagatatatatctctatatgcttcgctgcttagatctgaccctagtcgggcagtggttgaagataacaaagacattgaagaactagatcgtgaacttgatattgaacttatactgcagtggaggatgttggctcacaagtttgtagagcagtcattagagtcagaacatgactcgaggaaacaaaaagcaaagaaatcttggtggtctggatggggcagtcagtctctgaaagatgagaatgagccatttagtttcactgatgaagactggaagcagttaaatgaaatcatcggttataaggaaagtgatgatagactgttggttacagttgatgaaaagatggatgctcttcagacttccttgatcatttctatgaaacgtaatgccactaagcttattgatgagtctcaggagtgtcttgctgaattatcgtgtgaaggcatcggttgctcaataaagctctatccagaaacaaaagtttttgacctgaacttgggatcatataagttatcaacgccaaacggtctccttgctgagagtgcaagtgcttatgattctttagttggcgtgttctgtttcaagccttttgacaccaaggtggactggagctttgttgctaaggcttctccctgttatgtgacttatctaaaggatgcaattgatcaaattataaagttttttcggagtaatacagttagtcagaagatagctatggatactgcagctgcagtgcagatgacaattgatggagtcaagcgcacagctcagcaacaagtgaaccgggcattgaaggaccattccaggttttataatttttgttttgctaatgtaatcgatggagtcaagcgcacagctcagcaacaagtgaaccgggcattgaaggaccattccaggtttttgttggacttggatatcgcggctcctaagattaccattcctactgacttttgtcccgacaatattcatccaacaaaactgatgctggacttgggaaatttggttattggtaccaaggatgactatgagcatggatcattgaaggagcaagatatgtatctgcagtttaatttggttctaagtgatgtatctgcttttttggttgatggtgactactgttggggcaaatctcctgcaaaaaattctgctggttcttccaactcagatggtgttagtttgttgccttttattgataagtgtggggtaattctaacgtttcaacagattagattagagagtccatcctatcctgcaacacgactagctgtacgggtgccatcgttggggtttcacttctccccagctcggtatcatcgattgatgcaagtagttaagatctttaaggaggaggatagtgagacgtcagatctcgttggcccttggaatcaagctgactttgaggggtggtcggctcttcttgttttcaagggtctgggaaatagggaagcagtgtggcagcggcgctacctatgcttagttggccctttcctttatgtacttgagagccctagctcaaaatcgtacaagcaatgtatcagcttacaagggaaacacatatatgaggttcccccagagtctgttggtgataccgatcttgttttggctgtctgtgattctacacgagcgaataacaaggtcgtggaggatgcaaatgcactaatcttgctatttgattctgatgattcaaagaaaacttggcacagccggttgcggggggctgtctatcatgcatcagcgtctgctcctgtaactggtctatccgatacttcgtcagaatctgaggattcagtaatggagcttaatggaaatcctgatatgggtgatttatcaaaaatggagaagatattcataactggtgttcttgatgaactaaaggtctgcgtcagttatagttatcagcgtgaccaaaatatcatgaaggtgctcctaactgaagaaaggcgcttatttgaacttcgagcgataggtggtcaggttgaggtatcagttagatcgagtgacatctttattggaactgttttaaaatctttggagattgaagacttggtttctgggcacaccctctcccagccgcgttatctggcaagatcattcatccatagtgcagaaacacttataacctctgatgccccaggaaatcagagttttgatggttctggttcaattcgtttggaaggcgatgataaattctatgaggcaacagataatttggtcgactctggaaatttgatattgaaatctcctagttttactcgtattgctggtctactcccaagtgatggagttcaaacaacaaacgacattgcactcgatgattcaatggatagctttgtgaaagctcaagtagttatctatgaccagaactctcctctgtatcataatatggatatgcaggtcacagtcaccctggctaccttatcatttttctgtcgtcgaccaacaattcttgcaataatggaatttgcaaattctattaccatcaaggatgaaagctgtgaagctttcagtgacagttcttcaacaacagctacgaatcatgacaccagagatgatgcagtggatggttcgcaatcaatggcagtcagcaagcctgctgttaaaggattgctgggaaaggggaagtccagagttgtatttaatataacattaaatatggcccgtgctcagattttattaatgaatgaggatgaaactaaacttgctgttctgtcacaagataatttacttacggatattaaggtatttccatcctcttttagtattaaggcatccctcggaaatcttaaggtaagtgatgagagtcttccaagcagccatatgtatttctgggcgtgcgatatgagaaatccaggaggcagctcatttgttgaggtcacagtcaccctggctaccttatcatttttctgtcgtcgaccaacaattcttgcaataatggaatttgcaaattctattaccatcaaggatgaaagctgtgaagctttcagtgacagttcttcaacaacagctacgaatcatgacaccagagatgatgcagtggatggttcgcaatcaatggcagtcagcaagcctgctgttaaaggattgctgggaaaggggaagtccagagttgtatttaatataacattaaatatggcccgtgctcagattttattaatgaatgaggatgaaactaaacttgctgttctgtcacaagataatttacttacggatattaaggtatttccatcctcttttagtattaaggcatccctcggaaatcttaaggtaagtgatgagagtcttccaagcagccatatgtatttctgggcgtgcgatatgagaaatccaggaggcagctcatttgttgagttggttttcacttcatttagtgccgatgatgaagactttgaaggttatgagtatagcctctatggtcagctctcagaagtacgcattgtatatttgaatcgatttatccaggagattgttagttatttcatgggtcttgttcccaaaaactcaaaaggagtcgtgaagctaaaagatcaagtaacaaattcagagaagtggtttacaactagtgattttgaaggatcacctgctataaagttggatctgtctctgaggaagcctattatattgatgcccaggagaacggacagtcttgactacttgaagcttgacattgttcatattactgtgaagagcacctctcaatggtttggtggaagcagaactacgaatcatgacaccagagatgatgcagtggatggttcgcaatcaatggcagtcagcaagcctgctgttaaaggattgctgggaaaggggaagtccagagttgtatttaatataacattaaatatggcccgtgctcagattttattaatgaatgaggatgaaactaaacttgctgttctgtcacaagataatttacttacggatattaaggtatttccatcctcttttagtattaaggcatccctcggaaatcttaaggtaagtgatgagagtcttccaagcagccatatgtatttctgggcgtgcgatatgagaaatccaggaggcagctcatttgttgagttggttttcacttcatttagtgccgatgatgaagactttgaaggttatgagtatagcctctatggtcagctctcagaagtacgcattattgttagttatttcatgggtcttgttcccaaaaactcaaaaggagtcgtgaagctaaaagatcaagtaacaaattcagagaagtggtttacaactagtgattttgaaggatcacctgctataaagttggatctgtctctgaggaagcctattatattgatgcccaggagaacggacagtcttgactacttgaagcttgacattgttcatattactgtgaagagcacctctcaatggtttggtggaagcagaagtgatattaatgctgttcatatggaagtattaacagttcaggttgaggatatcaatttgaatgttggttgtaaaggagaaataggtgaaagcataattcaagatgtgaagggagtatctgttgtgattcagaggtcacttagagacttgatgcatcaaataccaaatgttgaggttctaatcaagatggagaaattgaaggcagctttgtcaaacagggaataccagattattaccgattgtgctcagtctaatgtttctgaaactccacatactgtgcctccattgaaccttgattctgtgacatctttaagtgagggtgaagccatcactcaggatccaaatggtactgcttttccaattgcaagtggggaagcccgggtcatgatgaaagtttgtgtcgtcattgatttggttgaattgtgcttacatactggtctagggagtcttgccactgtgcaggttgaggatatcaatttgaatgttggttgtaaaggagaaataggtgaaagcataattcaagatgtgaagggagtatctgttgtgattcagaggtcacttagagacttgatgcatcaaataccaaatgttgaggttctaatcaagatggagaaattgaaggcagctttgtcaaacagggaataccagattattaccgattgtgctcagtctaatgtttctgaaactccacatactgtgcctccattgaaccttgattctgtgacatctttaagtgagggtgaagccatcactcaggatccaaatggtactgcttttccaattgcaagtggggaagcccgggtcatgatgaaagtttgtgtcgtcattgatttggttgaattgtgcttacatactggtctagggagtcttgccactgtgcaggttactggtgtatggctactctacaagacaaataccctgggggaaggtttcctgtcagcaacactcaagggtttcactgttcttgatgatcgtgacggtactaaagaagagtttaggttggcgataggaaagcctgagaatattggttcttgttccgtagattctgtgacttatgatgacacccagcacatcactgatggaaatgtcggaaacttgaatgatatcaatttagttcctacaatgctcattgtcgatgccaaattttgccagcttttaacatctgtttcattatgcatccaaaggccacaacttcttgttgcacttgattttctgctggctgttgctgagttttttgttcctacagtcactgatgcgctgtcaactgaagaagtaaagaactctgtttatggaattgatgcaataattttgaaccagtccacttacaagcaaccctccactgaattatctctcactccattaagacctttggtagttgatgatgaaatatatgatcattttgtttatgatggtaatggtggaatattgcatttgaaagataggcgggggtcctatgtctctgggcctagtacagaggctattatatacataggagatgggaaaagattgcaattcaaaaatgttgttatcaagaatgggctgtacttagattcatgtatatccatgggaagcaacagtagctattcagctttaaaggaagatcaagtctactttgcgggagaggatgaagttcctaatttgagttcctcaagagaaagtcccaataatctgccatctcaaagcattgcagtcaacaaatcaacggaatttatcattgagttgcaggtggttggcccagagctgacattttataatacatccgaaaaacttggggaatcactgatgctctctaaccaacttttacatgctcaattggatggattctgcaggcttgttttgaaaggcgatgccattgagatgaatgcaaatgtgcttggtttcactatggagagtaacggaataaggattttggagccttttgatacttctgtaaaatattctaatgcttctggaaggaccaatatacatatatctgtttcagatatatttatgaatttttcattcagcatcctaaggctgtttttagcagttgaagaggatattttggcttttttaagcacgacctcaaagaaaataacagtagtctgctcgcagtttgacaagattggaacaatagaagatccctccagtgaccaaatatatgccctttggagacctcatgcacctcctggttttgcagttcttggggattatctgacaccattggacaagccacctacaaaggcagttgttgctgtaaataccaatttttccagagtgaagaagcccctatcttacaaactaatttggccacctttaccttctggggagttgtctcttgatggtgtaaatgatttggatgcaataccaagtggtgtcctttctgatgaaagtagctgctccatttggtttcctgaagcgcctgctggatacgttgcattgggctgtgtggtttgtcctggaagagcacaaccaccattatcttctgctttttgtatctcggcttcattagtttctccttgttccctgagggactgcattgcaattaacaccaacgatccatatcagtcaagtttagcattctggcgagtggataattctgttgggacctttttgccggcagatcttagtacttctagcatcacaggtagagcgtatgatttgcgtcacatcatatttggatttcctgaaccctctgcaaaatcatccaatagtttgaatgcccatacttcttctcaaagtcacaatccgcagtcagaaagatcagcaacagtaaactctggtcgccggtatgaagctgttgctagttttcgtttgatatggtggaaccaagggtcaaattcaagaaaaaggctatcgatatggcgtccagttgttcctccgggaatgatatactttggtgatattgcagtgaaagggtatgaacctccaaatacctgcattgttcttcatgatactgaagatgaagagctttttaaagcccctcttgattaccaaattgttgggcaaattaaaaaacagaggggaatggagagtgtgtccttttggcttcctcaagctcccccagggtttgtttcccttggttgtattgcatgtaagggaacacctaagcaatctgatttcagctcattaagatgcattcgcagtgatttggtaactggggatgaatttttagaggagagtatatgggatacatctgatgccaagcttacgaaagattcattcagtgtatgggcagttggcaatgagttgagtacctttctagttcgtggtggtttgaagaaacctcctagaaggtttgctttaaaactggctgattctaatgtaccaactggttcagatcttactgtaatagatgcggaaattagaaccttttctgcagcactttttgatgactatggtggcttgatggttccattgtgtaatttgtctttaagtggaattgggttcggtttgcatggaagaacagactacttgaattcgactgtgagcttctctttggctgctagatcatataatgataagtatgaggtttgggagcctcttgttgagccggtagatggattcctaagataccaatatgatctcaatgcaccaagtgcaacatctcagttacgcgttacatcgactagggatctgaacttgaacgtttctgtgtctaatgctaatatgataattcaagcttatgcaagctggaacagtctcgttcatgtgcctgaatatcataaaaaaagggaagcattctctccaacagacggtgaacgatctgttattgacatccatcacaggagaaactacgatataatcccacaaaacaagctaggtcaggatatctttattcgagctactgagtcaagaggacttacaagcataattaagatgtcatctggagatgtgaggcctgtaaaagttcctgtttccaagaatatgttggattctcatttgaaaggaaaacttttccacaaagttagaacaatggtgactttcatcatagtggatgggcagttcccacgggttagagggttgacatctccccattataccttagcaatccgtctcattcctgaccagagtctccctagtgaaacagtgattcaccaacaaaatgcgcgcacttgcggaagcagctcgaatcacctctctccagagcttgagttggtcaaatggaatgaaatatttttctttaaagttgacgctccagactactactcagttgaattacttgtgacggacatggggaaagagcataaatatatgaaaactgtgtttgatagacgtcatagaacaagtcagccatgcccttgttacttcccacgggttagagggttgacatctccccattataccttagcaatccgtctcattcctgaccagagtctccctagtgaaacagtgattcaccaacaaaatgcgcgcacttgcggaagcagctcgaatcacctctctccagagcttgagttggtcaaatggaatgaaatatttttctttaaagttgacgctccagactactactcagttgaattacttgtgacggacatggggaaaggggtccctcttggattcttttcagcccctctaaagcagattgtggggaattttaatcatgattcatatccatatgataatgtaaagaagtggacatccattgaattgtcctcggcagagtctatggatgataaccataagaaattgggtgggaaaatcagatgtgctgtattgctgtcaccaagatctgaaggagagataagtgatcaatatgataacagtaaaacaaaatctggatttatccagatcagtccgagaagggaaggaccttggactactgtgaggctaaactatgctgccccagctgcttgttggcggttaggcaatgatgttgttgctagtgaagtacgtgtaagggatggcaatagatatgtgaatattaggtctcttgtttcagtacgtaacagtaccgattttgtgcttgatttgtgtcttgtaccgaatgtttctatggaaaacgtgagtttaacagacaattcaagtactcccgagggtttacagacccatagcaacaaatttcagacagatgagttccttgaaactgaaaagtgcagcccgaccactggctgggttggctccatgattcaaccaagccaagatatgattgaaggtggaggatcccatcaggaatttcctacagttgagttaccaccaggctgggaatgggttgatgattggcatttggatatggcatctattgacactgctgatggctgggtctatgctccagatattgaaagtctaaaatggcccgagtcatttgatccattacggtttgtgaactatgcaaggcaaaggagatggatcaggaataggaagcaaagtactacgaatcaggaaatatatgttggaactttgaaacctggagataccataccccttcctctatgtgggttgacacaacctggattgtatgtcttgcgtttaaggccttctgatcttagcaatcatgatgagtattcttggagttcagcggtggatgggtctgaacaaccagaagagtctggttcgtcaaaagtatgtcctgagataagtgtatcgtctcttattgagtcagagaaactgttgtattgctcacagataagtagtacttcttcaagtggttcccacaaattgtggttttgtatgagtatacaagcgacagaaattgcaaaagacattcattctgatcctatccaggattggaatcttgtggttaagtccccgctatctatctccaattttcttccccttgcagctgagttttctgttcttgagatgcaagaaaatggtggttttgttgcctgcttcagaggggtgttttttccaggaaagaccgtgaatgtttttagtgctgacataagaaaacctttatacttttcactgcttccacaaagaggatggttgcccatccatgaagctgtccttttgtcccatcctcatgaagtttcatcaaaaacaataaacttgagaagttcgatatctggaaggattgttcaaattattctcgagcagaattccgttgaagagcgtccactgcaggctaagatcattagactctatgctccttattggttttcaattgctaggtgtcctcctctcacattcaggcttgtagatatagaagggaagaaaaatacacgaaagataggtggtttatttcagtctaaaaagattaatgaagttgtcctagaggaaataactgaagaggaaatatatgaaggtcatacaattgcttcagctctgaattttaaaatgctgggccttgctgtgtcgattgatcaatctggcaacaagcagtttggacctgttaaagatctttctccgcttggtgacatggatggatcattggatgtccctgcttatgatggcgaaggcaattgcatgcagctttttattaccacgaaaccatgcctgtatcaatctgttcctacaaaggtaatatctgtccgaccatttatgaccttcaccaacagacttggtcgtgatgtatatatcaagttatgcagcgaggatgaaccaaaagtccttcatccatgtgattcaagaatatcatttgtatatcgtgtatcagatggacctaataaacttcagctggacaaccaactccctctaacattgatgccagtgttgttggctcctgagccggactctgatatccatcatcctgttgtcaagatgactataacaatgcgtaatgaaaatactgatggcatccaggtctacccttacatttatatccgggtgactgagaagtgttggaggcttaatattcacgaaccaataatttgggctttggtggatctatacaataatctacagttagatcgtctgcctaaaagttctagtgtcacagaagttgatccagaaatacgcatcgacctagtagacatttcagaagttaggctaaaggtgtcactggaaacagcaccagctcagagacctcatggggttctaggagtatggagcccaatactatctgcagttggaaatgcattcaagattcaggtgcatctgaggagggtgatgcacaaagacagattcatgcggagaagttccattgtctctgccattggtaaccgcgtttggagagacttgatccataatcctctacatttgatattttcagttgatgttcttggtatgacaagttcaactttggcttctcttagtaaagggtttgctgagctctcaactgatgggcagtttactcacctacggtcaaaacaggtattttcaaggagaattacgggggtgggtgatggaatcattcaaggcacagaagcatttgtgcaaggtgttgcttttggcgtatctggagtagtgaaaaagccagttgagagtgcaagacagaacggtctgtcaggactttttcacgggattggacgtgcattcctgggagttattgtgcaaccaatgagtggggcattggactttttctccttgactgttgatggaattggagcaagttgttcgaaatgcctagaggtcttcaatagcaagacaacattccagagaattagaaacccccgggcaatccgtgccaatggtatacttacagagtattctgagagagaggcagttggacagatgattttataccttgcagaagcacatcggcactttggttgtactgaaatattcaaggagccctcaaagtttgcttggtctgattattatgaagagcattttgttgtgccttaccagcggattgttttagtgaccaataagcgagtgatgttgcttcagtgtttggctccagacaaaatggacaagaaaccgtgcaagattatgtgggatgtgccttgggaagagcttatggcagtggagttggctaaagcaggtcacaatcagccatctcacttgatcctccacctcaaaagttttaggagatcagagaattttgtccgggtcataaagtgcaatgttgaagatgaagtagagggaagggaaccccaggctgttagaatctgttctgcagcatgtaggatgtggaaagcaaaccagtctgccatgaaaagcctgatgttgaaggttccatcaagtcagcgacatgtgtattttgcctggtctgaagccgaggggagagaaaaacgtactctgaacaaagcagtgactaggctgagagagctcccgtcatacagttctgcatcagatggtagaagatttgttaaacacagcatcaacttttcaaagatttggagcagtgaacaagaatccagaggtcgatgcacattgtgcaaaaagcaggttttggaagatgctggattatgtagcatttggagacccatttgtcctgacgggtatgtctctattggagatattgctcacattggcagccatcctccaaacgttgctgcagtttaccgtaaaattgacaggttgtttgcacttccagttggatacgacctggtgtggagaaattgtttggatgactatgcaacaccagtatcaatttggcatcctagagctccagaaggattcgtatctcctggatgcatagctgtggctggttttgttgagccagaacctgatttggttcactgtgttgctatatcacttgctgaggagacagaatttgaagagcaaaaggtttggtcagcttcagattcttatccatgggcatgtcacatttatcaagtccgcagtgaagctcttcacttcgtggcattaagaacaagcaaggaagaatctgactggaaacctatgaggattcttgatgatcctcagcccctattgcagtcattgaaagagcactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chorein_N PH Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

4920

Amino Acids

548.35

Weight (kDa)

5.93

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_N PF12624 169 - 705 4.2e-104 VPS13-like family N-terminal region
VPS13_N PF12624 726 - 871 8.3e-06 VPS13-like family N-terminal region
PH PF00169 1000 - 1108 1.3e-07 PH domain
Vps62 PF06101 2696 - 2856 3.2e-09 Vacuolar protein sorting-associated protein 62
Vps62 PF06101 2926 - 3073 3.5e-11 Vacuolar protein sorting-associated protein 62
VPS13_VAB PF25036 3764 - 4026 2.9e-08 Vacuolar-sorting associated protein 13, adaptor binding domain
VPS13_C PF25037 4508 - 4616 1.2e-12 Intermembrane lipid transfer protein VPS13, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 1902, 2276, 2415, 3719, 7752
AarI CACCTGC 3 cut(s) 5188, 5872, 7714
AasI GACNNNNNNGTC 3 cut(s) 13359, 14295, 14309
AatII GACGTC 3 cut(s) 340, 2966, 10258
Acc36I ACCTGC 9 cut(s) 3985, 5188, 5872, 6451, 6871, 7714, 8924, 13835, 14242
Acc65I GGTACC 2 cut(s) 540, 2612
AccB1I GGYRCC 3 cut(s) 540, 2612, 2875
AccB7I CCANNNNNTGG 3 cut(s) 3881, 11091, 13424
AccII CGCG 8 cut(s) 251, 464, 2522, 3676, 9597, 10090, 10393, 13086
AclI AACGTT 3 cut(s) 2816, 9627, 14355
AcoI YGGCCR 3 cut(s) 494, 724, 8288
AcvI CACGTG 1 cut(s) 221
AcyI GRCGYC 4 cut(s) 337, 2963, 8845, 10255
AdeI CACNNNGTG 3 cut(s) 9578, 14241, 14668
AflII CTTAAG 4 cut(s) 701, 4385, 4862, 5573
AflIII ACRYGT 5 cut(s) 462, 1477, 9595, 10873, 14056
AhlI ACTAGT 2 cut(s) 5159, 5843
AjiI CACGTC 2 cut(s) 446, 13391
AjuI GAANNNNNNNTTGG 2 cut(s) 11355, 11387
AleI CACNNNNGTG 1 cut(s) 2201
AloI GAACNNNNNNTCC 6 cut(s) 9968, 10000, 11982, 12014, 12513, 12545
Alw44I GTGCAC 1 cut(s) 1083
AlwNI CAGNNNCTG 7 cut(s) 594, 1831, 3179, 3341, 3380, 7250, 14534
Ama87I CYCGRG 7 cut(s) 1777, 6378, 6798, 11012, 11276, 12011, 13533
ApaI GGGCCC 1 cut(s) 77
ApaLI GTGCAC 1 cut(s) 1083
ArsI GACNNNNNNTTYG 8 cut(s) 460, 492, 1369, 1401, 2198, 2230, 14372, 14404
AseI ATTAAT 5 cut(s) 4277, 4754, 5465, 6009, 12180
Asp700I GAANNNNTTC 4 cut(s) 3446, 9352, 13975, 14658
Asp718I GGTACC 2 cut(s) 540, 2612
AspA2I CCTAGG 1 cut(s) 1499
AsuII TTCGAA 2 cut(s) 88, 13478
AvaI CYCGRG 7 cut(s) 1777, 6378, 6798, 11012, 11276, 12011, 13533
AvrII CCTAGG 1 cut(s) 1499
AxyI CCTNAGG 2 cut(s) 8012, 8507
BaeGI GKGCMC 5 cut(s) 50, 77, 241, 1087, 3660
BaeI ACNNNNGTAYC 4 cut(s) 7914, 7947, 14436, 14469
BalI TGGCCA 2 cut(s) 726, 8290
BamHI GGATCC 5 cut(s) 730, 908, 6337, 6757, 11141
BanI GGYRCC 3 cut(s) 540, 2612, 2875
BanII GRGCYC 7 cut(s) 77, 3110, 12974, 13194, 13656, 14136, 14476
BarI GAAGNNNNNNTAC 8 cut(s) 1505, 1537, 5302, 5334, 8467, 8499, 11602, 11634
BauI CACGAG 1 cut(s) 3222
BbrPI CACGTG 1 cut(s) 221
BbsI GAAGAC 8 cut(s) 1875, 3648, 4397, 4874, 4989, 5585, 5700, 13486
BbvCI CCTCAGC 2 cut(s) 14568, 14730
BcgI CGANNNNNNTGC 4 cut(s) 514, 548, 13458, 13492
BciVI GTATCC 5 cut(s) 1394, 2314, 8402, 9163, 14399
BclI TGATCA 6 cut(s) 915, 1968, 2266, 7393, 10729, 12283
BcuI ACTAGT 2 cut(s) 5159, 5843
BfoI RGCGCY 3 cut(s) 3067, 3547, 8402
BfrI CTTAAG 4 cut(s) 701, 4385, 4862, 5573
BfuAI ACCTGC 9 cut(s) 3985, 5188, 5872, 6451, 6871, 7714, 8924, 13835, 14242
BfuI GTATCC 5 cut(s) 1394, 2314, 8402, 9163, 14399
BglII AGATCT 7 cut(s) 1637, 2937, 2968, 8602, 9319, 10709, 12319
BlnI CCTAGG 1 cut(s) 1499
BlpI GCTNAGC 4 cut(s) 771, 2370, 2460, 13188
BmcAI AGTACT 4 cut(s) 8611, 11008, 11350, 11614
BmeT110I CYCGRG 7 cut(s) 1777, 6378, 6798, 11012, 11276, 12011, 13533
BmgBI CACGTC 2 cut(s) 446, 13391
BmrI ACTGGG 2 cut(s) 1086, 9147
BmuI ACTGGG 2 cut(s) 1086, 9147
BoxI GACNNNNGTC 4 cut(s) 1647, 2553, 10050, 10353
BpiI GAAGAC 8 cut(s) 1875, 3648, 4397, 4874, 4989, 5585, 5700, 13486
BplI GAGNNNNNCTC 6 cut(s) 5278, 5310, 5962, 5994, 12638, 12670
Bpu10I CCTNAGC 7 cut(s) 2223, 9087, 10024, 10327, 12645, 14568, 14730
Bpu1102I GCTNAGC 4 cut(s) 771, 2370, 2460, 13188
Bpu14I TTCGAA 2 cut(s) 88, 13478
Bsa29I ATCGAT 4 cut(s) 2439, 2917, 5050, 8837
BsaAI YACGTR 5 cut(s) 221, 556, 1601, 10874, 10925
BsaBI GATNNNNATC 9 cut(s) 1612, 2658, 3192, 8541, 10590, 11709, 12663, 14488, 14616
BsaHI GRCGYC 4 cut(s) 337, 2963, 8845, 10255
BsaI GGTCTC 5 cut(s) 2135, 8146, 10914, 12937, 14281
Bse118I RCCGGY 7 cut(s) 59, 121, 496, 3306, 8596, 8766, 9532
Bse21I CCTNAGG 2 cut(s) 8012, 8507
Bse3DI GCAATG 8 cut(s) 1178, 3137, 3903, 7683, 8517, 9229, 10855, 13933
Bse8I GATNNNNATC 9 cut(s) 1612, 2658, 3192, 8541, 10590, 11709, 12663, 14488, 14616
BseCI ATCGAT 4 cut(s) 2439, 2917, 5050, 8837
BseJI GATNNNNATC 9 cut(s) 1612, 2658, 3192, 8541, 10590, 11709, 12663, 14488, 14616
BseMI GCAATG 8 cut(s) 1178, 3137, 3903, 7683, 8517, 9229, 10855, 13933
BsePI GCGCGC 2 cut(s) 10088, 10391
BseRI GAGGAG 6 cut(s) 2963, 3960, 9170, 12090, 13036, 14585
BseSI GKGCMC 5 cut(s) 50, 77, 241, 1087, 3660
BseX3I CGGCCG 1 cut(s) 494
BsgI GTGCAG 6 cut(s) 857, 2357, 3726, 6479, 6899, 11095
Bsh1236I CGCG 8 cut(s) 251, 464, 2522, 3676, 9597, 10090, 10393, 13086
Bsh1285I CGRYCG 1 cut(s) 497
BshNI GGYRCC 3 cut(s) 540, 2612, 2875
BshVI ATCGAT 4 cut(s) 2439, 2917, 5050, 8837
BsiEI CGRYCG 1 cut(s) 497
BsiHKCI CYCGRG 7 cut(s) 1777, 6378, 6798, 11012, 11276, 12011, 13533
BsmBI CGTCTC 4 cut(s) 2954, 10040, 10343, 11571
BsmI GAATGC 8 cut(s) 652, 7867, 8558, 8704, 9117, 9719, 12998, 13394
Bso31I GGTCTC 5 cut(s) 2135, 8146, 10914, 12937, 14281
BsoBI CYCGRG 7 cut(s) 1777, 6378, 6798, 11012, 11276, 12011, 13533
Bsp119I TTCGAA 2 cut(s) 88, 13478
Bsp120I GGGCCC 1 cut(s) 73
Bsp1407I TGTACA 1 cut(s) 811
Bsp1720I GCTNAGC 4 cut(s) 771, 2370, 2460, 13188
Bsp19I CCATGG 4 cut(s) 710, 1154, 7563, 14621
BspDI ATCGAT 4 cut(s) 2439, 2917, 5050, 8837
BspFNI CGCG 8 cut(s) 251, 464, 2522, 3676, 9597, 10090, 10393, 13086
BspMI ACCTGC 9 cut(s) 3985, 5188, 5872, 6451, 6871, 7714, 8924, 13835, 14242
BspT104I TTCGAA 2 cut(s) 88, 13478
BspT107I GGYRCC 3 cut(s) 540, 2612, 2875
BspTI CTTAAG 4 cut(s) 701, 4385, 4862, 5573
BspTNI GGTCTC 5 cut(s) 2135, 8146, 10914, 12937, 14281
BsrDI GCAATG 8 cut(s) 1178, 3137, 3903, 7683, 8517, 9229, 10855, 13933
BsrFI RCCGGY 7 cut(s) 59, 121, 496, 3306, 8596, 8766, 9532
BsrGI TGTACA 1 cut(s) 811
BssAI RCCGGY 7 cut(s) 59, 121, 496, 3306, 8596, 8766, 9532
BssHII GCGCGC 2 cut(s) 10088, 10391
BssNAI GTATAC 2 cut(s) 11657, 13556
BssNI GRCGYC 4 cut(s) 337, 2963, 8845, 10255
BssSI CACGAG 1 cut(s) 3222
Bst1107I GTATAC 2 cut(s) 11657, 13556
Bst2BI CACGAG 1 cut(s) 3222
BstACI GRCGYC 4 cut(s) 337, 2963, 8845, 10255
BstAFI CTTAAG 4 cut(s) 701, 4385, 4862, 5573
BstAPI GCANNNNNTGC 6 cut(s) 647, 2052, 8231, 9578, 13544, 14241
BstAUI TGTACA 1 cut(s) 811
BstBAI YACGTR 5 cut(s) 221, 556, 1601, 10874, 10925
BstBI TTCGAA 2 cut(s) 88, 13478
BstDSI CCRYGG 6 cut(s) 710, 1154, 7563, 9989, 10292, 14621
BstEII GGTNACC 1 cut(s) 13079
BstFNI CGCG 8 cut(s) 251, 464, 2522, 3676, 9597, 10090, 10393, 13086
BstH2I RGCGCY 3 cut(s) 3067, 3547, 8402
BstMCI CGRYCG 1 cut(s) 497
BstNSI RCATGY 8 cut(s) 1163, 1481, 7808, 9075, 12391, 13989, 14060, 14631
BstPAI GACNNNNGTC 4 cut(s) 1647, 2553, 10050, 10353
BstPI GGTNACC 1 cut(s) 13079
BstSLI GKGCMC 5 cut(s) 50, 77, 241, 1087, 3660
BstSNI TACGTA 2 cut(s) 1601, 10925
BstUI CGCG 8 cut(s) 251, 464, 2522, 3676, 9597, 10090, 10393, 13086
BstV2I GAAGAC 8 cut(s) 1875, 3648, 4397, 4874, 4989, 5585, 5700, 13486
BstXI CCANNNNNNTGG 4 cut(s) 1197, 8981, 11181, 12792
BstZ17I GTATAC 2 cut(s) 11657, 13556
BstZI CGGCCG 1 cut(s) 494
Bsu15I ATCGAT 4 cut(s) 2439, 2917, 5050, 8837
Bsu36I CCTNAGG 2 cut(s) 8012, 8507
BsuI GTATCC 5 cut(s) 1394, 2314, 8402, 9163, 14399
BsuTUI ATCGAT 4 cut(s) 2439, 2917, 5050, 8837
BtgI CCRYGG 6 cut(s) 710, 1154, 7563, 9989, 10292, 14621
BtgZI GCGATG 2 cut(s) 3795, 7860
BtrI CACGTC 2 cut(s) 446, 13391
BveI ACCTGC 9 cut(s) 3985, 5188, 5872, 6451, 6871, 7714, 8924, 13835, 14242
CaiI CAGNNNCTG 7 cut(s) 594, 1831, 3179, 3341, 3380, 7250, 14534
Cfr10I RCCGGY 7 cut(s) 59, 121, 496, 3306, 8596, 8766, 9532
Cfr9I CCCGGG 3 cut(s) 6378, 6798, 13533
ClaI ATCGAT 4 cut(s) 2439, 2917, 5050, 8837
CseI GACGC 8 cut(s) 453, 3327, 3472, 8633, 8834, 10181, 10484, 12023
CsiI ACCWGGT 1 cut(s) 14412
DraI TTTAAA 8 cut(s) 1213, 3624, 7593, 8956, 9285, 10165, 10468, 12256
DraIII CACNNNGTG 3 cut(s) 9578, 14241, 14668
DrdI GACNNNNNNGTC 3 cut(s) 13359, 14295, 14309
DseDI GACNNNNNNGTC 3 cut(s) 13359, 14295, 14309
EaeI YGGCCR 3 cut(s) 494, 724, 8288
EagI CGGCCG 1 cut(s) 494
Ecl136II GAGCTC 3 cut(s) 13192, 14134, 14474
EclXI CGGCCG 1 cut(s) 494
Eco105I TACGTA 2 cut(s) 1601, 10925
Eco147I AGGCCT 2 cut(s) 9880, 11455
Eco24I GRGCYC 7 cut(s) 77, 3110, 12974, 13194, 13656, 14136, 14476
Eco31I GGTCTC 5 cut(s) 2135, 8146, 10914, 12937, 14281
Eco32I GATATC 7 cut(s) 2518, 6052, 6472, 7105, 9811, 11985, 12661
Eco52I CGGCCG 1 cut(s) 494
Eco53kI GAGCTC 3 cut(s) 13192, 14134, 14474
Eco72I CACGTG 1 cut(s) 221
Eco81I CCTNAGG 2 cut(s) 8012, 8507
Eco88I CYCGRG 7 cut(s) 1777, 6378, 6798, 11012, 11276, 12011, 13533
Eco91I GGTNACC 1 cut(s) 13079
EcoICRI GAGCTC 3 cut(s) 13192, 14134, 14474
EcoO109I RGGNCCY 5 cut(s) 577, 7449, 8586, 10528, 10790
EcoO65I GGTNACC 1 cut(s) 13079
EcoRI GAATTC 2 cut(s) 9457, 12019
EcoRV GATATC 7 cut(s) 2518, 6052, 6472, 7105, 9811, 11985, 12661
EcoT22I ATGCAT 7 cut(s) 3334, 6156, 6576, 7178, 9119, 13000, 14504
EcoT38I GRGCYC 7 cut(s) 77, 3110, 12974, 13194, 13656, 14136, 14476
Esp3I CGTCTC 4 cut(s) 2954, 10040, 10343, 11571
FauI CCCGC 6 cut(s) 397, 1003, 3306, 7437, 7609, 11736
FauNDI CATATG 6 cut(s) 322, 4419, 4896, 5607, 6021, 10597
FbaI TGATCA 6 cut(s) 915, 1968, 2266, 7393, 10729, 12283
FriOI GRGCYC 7 cut(s) 77, 3110, 12974, 13194, 13656, 14136, 14476
HaeII RGCGCY 3 cut(s) 3067, 3547, 8402
HgaI GACGC 8 cut(s) 453, 3327, 3472, 8633, 8834, 10181, 10484, 12023
Hin1I GRCGYC 4 cut(s) 337, 2963, 8845, 10255
HindIII AAGCTT 7 cut(s) 1997, 4101, 4578, 5258, 5942, 9185, 9660
HpaI GTTAAC 1 cut(s) 1165
Hpy99I CGWCG 5 cut(s) 339, 469, 472, 4038, 4515
Hsp92I GRCGYC 4 cut(s) 337, 2963, 8845, 10255
KflI GGGWCCC 1 cut(s) 10528
KpnI GGTACC 2 cut(s) 544, 2616
KroI GCCGGC 3 cut(s) 59, 496, 8596
KroNI GCCGGC 3 cut(s) 61, 498, 8598
Ksp22I TGATCA 6 cut(s) 915, 1968, 2266, 7393, 10729, 12283
KspAI GTTAAC 1 cut(s) 1165
MabI ACCWGGT 1 cut(s) 14412
MfeI CAATTG 8 cut(s) 2262, 2346, 6360, 6780, 7811, 12084, 12234, 12382
MlsI TGGCCA 2 cut(s) 726, 8290
MluI ACGCGT 2 cut(s) 462, 9595
MluNI TGGCCA 2 cut(s) 726, 8290
Mox20I TGGCCA 2 cut(s) 726, 8290
Mph1103I ATGCAT 7 cut(s) 3334, 6156, 6576, 7178, 9119, 13000, 14504
MroNI GCCGGC 3 cut(s) 59, 496, 8596
MroXI GAANNNNTTC 4 cut(s) 3446, 9352, 13975, 14658
MscI TGGCCA 2 cut(s) 726, 8290
Msp20I TGGCCA 2 cut(s) 726, 8290
MspA1I CMGCKG 9 cut(s) 1010, 1450, 2330, 3061, 10829, 11498, 11762, 12598, 13711
MspCI CTTAAG 4 cut(s) 701, 4385, 4862, 5573
MunI CAATTG 8 cut(s) 2262, 2346, 6360, 6780, 7811, 12084, 12234, 12382
Mva1269I GAATGC 8 cut(s) 652, 7867, 8558, 8704, 9117, 9719, 12998, 13394
MvnI CGCG 8 cut(s) 251, 464, 2522, 3676, 9597, 10090, 10393, 13086
NaeI GCCGGC 3 cut(s) 61, 498, 8598
NcoI CCATGG 4 cut(s) 710, 1154, 7563, 14621
NdeI CATATG 6 cut(s) 322, 4419, 4896, 5607, 6021, 10597
NgoMIV GCCGGC 3 cut(s) 59, 496, 8596
NmeAIII GCCGAG 3 cut(s) 8459, 10616, 14107
NsiI ATGCAT 7 cut(s) 3334, 6156, 6576, 7178, 9119, 13000, 14504
NspI RCATGY 8 cut(s) 1163, 1481, 7808, 9075, 12391, 13989, 14060, 14631
NspV TTCGAA 2 cut(s) 88, 13478
OliI CACNNNNGTG 1 cut(s) 2201
PaeI GCATGC 1 cut(s) 12391
PaeR7I CTCGAG 2 cut(s) 1777, 12011
PaqCI CACCTGC 3 cut(s) 5188, 5872, 7714
PasI CCCWGGG 2 cut(s) 6919, 9044
PauI GCGCGC 2 cut(s) 10088, 10391
PceI AGGCCT 2 cut(s) 9880, 11455
PciI ACATGT 2 cut(s) 1477, 14056
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 476, 2914
PctI GAATGC 8 cut(s) 652, 7867, 8558, 8704, 9117, 9719, 12998, 13394
PdiI GCCGGC 3 cut(s) 61, 498, 8598
PdmI GAANNNNTTC 4 cut(s) 3446, 9352, 13975, 14658
PflFI GACNNNGTC 1 cut(s) 12483
PflMI CCANNNNNTGG 3 cut(s) 3881, 11091, 13424
PfoI TCCNGGA 9 cut(s) 4450, 4927, 5058, 5638, 8437, 8860, 11700, 11830, 14494
PmaCI CACGTG 1 cut(s) 221
PmlI CACGTG 1 cut(s) 221
Ppu21I YACGTR 5 cut(s) 221, 556, 1601, 10874, 10925
PpuMI RGGWCCY 4 cut(s) 7449, 8586, 10528, 10790
PscI ACATGT 2 cut(s) 1477, 14056
PshAI GACNNNNGTC 4 cut(s) 1647, 2553, 10050, 10353
PshBI ATTAAT 5 cut(s) 4277, 4754, 5465, 6009, 12180
PsiI TTATAA 5 cut(s) 1902, 2276, 2415, 3719, 7752
Psp124BI GAGCTC 3 cut(s) 13194, 14136, 14476
Psp1406I AACGTT 3 cut(s) 2816, 9627, 14355
Psp5II RGGWCCY 4 cut(s) 7449, 8586, 10528, 10790
PspCI CACGTG 1 cut(s) 221
PspEI GGTNACC 1 cut(s) 13079
PspOMI GGGCCC 1 cut(s) 73
PspPPI RGGWCCY 4 cut(s) 7449, 8586, 10528, 10790
PspXI VCTCGAGB 1 cut(s) 1777
PsrI GAACNNNNNNTAC 4 cut(s) 6021, 6053, 13138, 13170
PstNI CAGNNNCTG 7 cut(s) 594, 1831, 3179, 3341, 3380, 7250, 14534
PsyI GACNNNGTC 1 cut(s) 12483
PteI GCGCGC 2 cut(s) 10088, 10391
PvuII CAGCTG 4 cut(s) 2330, 10829, 11762, 12598
SacI GAGCTC 3 cut(s) 13194, 14136, 14476
SalI GTCGAC 4 cut(s) 466, 3815, 4035, 4512
ScaI AGTACT 4 cut(s) 8611, 11008, 11350, 11614
SexAI ACCWGGT 1 cut(s) 14412
Sfr274I CTCGAG 2 cut(s) 1777, 12011
SfuI TTCGAA 2 cut(s) 88, 13478
SgrDI CGTCGACG 1 cut(s) 466
SlaI CTCGAG 2 cut(s) 1777, 12011
SmaI CCCGGG 3 cut(s) 6380, 6800, 13535
SnaBI TACGTA 2 cut(s) 1601, 10925
SpeI ACTAGT 2 cut(s) 5159, 5843
SphI GCATGC 1 cut(s) 12391
SseBI AGGCCT 2 cut(s) 9880, 11455
SstI GAGCTC 3 cut(s) 13194, 14136, 14476
StuI AGGCCT 2 cut(s) 9880, 11455
TaqII GACCGA 1 cut(s) 171
TauI GCSGC 4 cut(s) 441, 2525, 3064, 3676
TspMI CCCGGG 3 cut(s) 6378, 6798, 13533
Tth111I GACNNNGTC 1 cut(s) 12483
Van91I CCANNNNNTGG 3 cut(s) 3881, 11091, 13424
Vha464I CTTAAG 4 cut(s) 701, 4385, 4862, 5573
VneI GTGCAC 1 cut(s) 1083
VspI ATTAAT 5 cut(s) 4277, 4754, 5465, 6009, 12180
XceI RCATGY 8 cut(s) 1163, 1481, 7808, 9075, 12391, 13989, 14060, 14631
XcmI CCANNNNNNNNNTGG 6 cut(s) 2503, 9907, 10833, 11184, 11901, 14457
XhoI CTCGAG 2 cut(s) 1777, 12011
XmaI CCCGGG 3 cut(s) 6378, 6798, 13533
XmaJI CCTAGG 1 cut(s) 1499
XmnI GAANNNNTTC 4 cut(s) 3446, 9352, 13975, 14658
ZraI GACGTC 3 cut(s) 338, 2964, 10256
ZrmI AGTACT 4 cut(s) 8611, 11008, 11350, 11614
Zsp2I ATGCAT 7 cut(s) 3334, 6156, 6576, 7178, 9119, 13000, 14504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.