Rmu_sc0004989.1_g000008

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004989.1
OG0012579
Physical Location & Seq
Reverse (-)
45691 .. 46653
963 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 609 bp
atggctgtttccaacatacagagagatgcgattgactctattgttcaatatctcgatagaatcaacctgaggcaatccgcaagaatgtacgcggactgttttgttcgtggagcctttgaagccgctgaggactacatgatgggcttcaccggagtccatgacaacatattttcaataaatgttgtttttgagctccgaaatcatcgtgaggaggcgcgagtcatcatccgcgaaaaacttcaaccattggggatctacaaccaagagttggttggcttcgctacttatctagtgaatatgcataacttcaggatgcatgaagcttttttttactacggggatccagagtaccacaggagggagagggtagagttcattagggtgaacttggagtccaatcctctattgtttgggaaaattcggtatcccattcccaacgtgaataatgcagtcatcgtaccacctcaagttccaacagctctagcagttcctgccacttcactaattccaattccagcacctcgagcagctccccggccattagcgttaccgggaccgaggaattttagtgttactccgaggtggtttcctagaactcggaggacctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04140 map04137 map04140
Pfam Domains
Protein Families
WD40

Protein Analysis

202

Amino Acids

23.34

Weight (kDa)

9.6

Isoelectric Point (pI)

53.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR1-like_2nd PF21889 29 - 139 1.4e-12 TPR1-like, CTLH-containing domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 268
AccII CGCG 3 cut(s) 92, 217, 231
AciI CCGC 4 cut(s) 78, 92, 123, 229
AclWI GGATC 3 cut(s) 260, 335, 348
AcoI YGGCCR 1 cut(s) 536
AcsI RAATTY 2 cut(s) 417, 562
AcuI CTGAAG 1 cut(s) 292
AfaI GTAC 3 cut(s) 89, 350, 459
AfiI CCNNNNNNNGG 2 cut(s) 268, 358
AgsI TTSAA 4 cut(s) 47, 119, 174, 242
AloI GAACNNNNNNTCC 2 cut(s) 377, 409
AluBI AGCT 4 cut(s) 193, 323, 479, 530
AluI AGCT 4 cut(s) 193, 323, 479, 530
Alw21I GWGCWC 1 cut(s) 195
AlwI GGATC 3 cut(s) 260, 335, 348
AlwNI CAGNNNCTG 1 cut(s) 491
Ama87I CYCGRG 1 cut(s) 522
AoxI GGCC 1 cut(s) 536
ApeKI GCWGC 1 cut(s) 527
ApoI RAATTY 2 cut(s) 417, 562
Asp700I GAANNNNTTC 1 cut(s) 237
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 2 cut(s) 554, 603
AsuC2I CCSGG 2 cut(s) 535, 552
AsuHPI GGTGA 2 cut(s) 139, 394
AvaI CYCGRG 1 cut(s) 522
AvaII GGWCC 2 cut(s) 554, 603
AxyI CCTNAGG 1 cut(s) 68
BamHI GGATCC 1 cut(s) 340
BanII GRGCYC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 195
BbvCI CCTCAGC 1 cut(s) 126
BbvI GCAGC 1 cut(s) 539
BccI CCATC 1 cut(s) 133
BciVI GTATCC 1 cut(s) 435
BcnI CCSGG 2 cut(s) 535, 552
BfaI CTAG 3 cut(s) 290, 482, 591
BfuI GTATCC 1 cut(s) 435
BisI GCNGC 2 cut(s) 123, 528
BlsI GCNGC 2 cut(s) 124, 529
Bme1390I CCNGG 2 cut(s) 535, 552
Bme18I GGWCC 2 cut(s) 554, 603
BmeT110I CYCGRG 1 cut(s) 522
BmgT120I GGNCC 2 cut(s) 554, 603
BmiI GGNNCC 3 cut(s) 112, 342, 555
BmrFI CCNGG 2 cut(s) 535, 552
BmsI GCATC 2 cut(s) 16, 303
Bpu10I CCTNAGC 1 cut(s) 126
BpuEI CTTGAG 1 cut(s) 450
BpuMI CCSGG 2 cut(s) 535, 552
BsaJI CCNNGG 3 cut(s) 533, 557, 578
BsaWI WCCGGW 1 cut(s) 149
Bsc4I CCNNNNNNNGG 2 cut(s) 268, 358
Bse21I CCTNAGG 1 cut(s) 68
BseDI CCNNGG 3 cut(s) 533, 557, 578
BseGI GGATG 2 cut(s) 225, 318
BseLI CCNNNNNNNGG 2 cut(s) 268, 358
BseMII CTCAG 2 cut(s) 59, 117
BseRI GAGGAG 1 cut(s) 224
BseXI GCAGC 1 cut(s) 539
Bsh1236I CGCG 3 cut(s) 92, 217, 231
BshFI GGCC 1 cut(s) 538
BsiHKAI GWGCWC 1 cut(s) 195
BsiHKCI CYCGRG 1 cut(s) 522
BsiSI CCGG 3 cut(s) 150, 535, 551
BslFI GGGAC 1 cut(s) 567
BslI CCNNNNNNNGG 2 cut(s) 268, 358
BsmFI GGGAC 1 cut(s) 567
BsnI GGCC 1 cut(s) 538
BsoBI CYCGRG 1 cut(s) 522
Bsp1286I GDGCHC 1 cut(s) 195
Bsp143I GATC 2 cut(s) 252, 340
BspACI CCGC 4 cut(s) 78, 92, 123, 229
BspANI GGCC 1 cut(s) 538
BspCNI CTCAG 2 cut(s) 60, 118
BspFNI CGCG 3 cut(s) 92, 217, 231
BspLI GGNNCC 3 cut(s) 112, 342, 555
BspPI GGATC 3 cut(s) 260, 335, 348
BssECI CCNNGG 3 cut(s) 533, 557, 578
BssMI GATC 2 cut(s) 252, 340
Bst4CI ACNGT 1 cut(s) 98
BstAPI GCANNNNNTGC 1 cut(s) 491
BstDEI CTNAG 2 cut(s) 68, 126
BstF5I GGATG 2 cut(s) 225, 318
BstFNI CGCG 3 cut(s) 92, 217, 231
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 2 cut(s) 255, 343
BstMBI GATC 2 cut(s) 252, 340
BstMWI GCNNNNNNNGC 3 cut(s) 119, 491, 524
BstSCI CCNGG 2 cut(s) 533, 550
BstUI CGCG 3 cut(s) 92, 217, 231
BstV1I GCAGC 1 cut(s) 539
BstX2I RGATCY 2 cut(s) 252, 340
BstYI RGATCY 2 cut(s) 252, 340
Bsu36I CCTNAGG 1 cut(s) 68
BsuI GTATCC 1 cut(s) 435
BsuRI GGCC 1 cut(s) 538
BtsCI GGATG 2 cut(s) 225, 318
CaiI CAGNNNCTG 1 cut(s) 491
CfoI GCGC 1 cut(s) 217
Cfr13I GGNCC 2 cut(s) 554, 603
Csp6I GTAC 3 cut(s) 88, 349, 458
CviAII CATG 3 cut(s) 136, 158, 317
CviQI GTAC 3 cut(s) 88, 349, 458
DdeI CTNAG 2 cut(s) 68, 126
DpnI GATC 2 cut(s) 254, 342
DpnII GATC 2 cut(s) 252, 340
EaeI YGGCCR 1 cut(s) 536
Ecl136II GAGCTC 1 cut(s) 193
Eco24I GRGCYC 1 cut(s) 195
Eco47I GGWCC 2 cut(s) 554, 603
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 292
Eco81I CCTNAGG 1 cut(s) 68
Eco88I CYCGRG 1 cut(s) 522
EcoICRI GAGCTC 1 cut(s) 193
EcoO109I RGGNCCY 1 cut(s) 603
EcoT22I ATGCAT 2 cut(s) 303, 318
EcoT38I GRGCYC 1 cut(s) 195
FaeI CATG 3 cut(s) 139, 161, 320
FaiI YATR 7 cut(s) 17, 137, 159, 167, 299, 303, 318
FaqI GGGAC 1 cut(s) 567
FatI CATG 3 cut(s) 135, 157, 316
Fnu4HI GCNGC 2 cut(s) 123, 528
FokI GGATG 2 cut(s) 212, 325
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 2 cut(s) 123, 528
FspBI CTAG 3 cut(s) 290, 482, 591
GlaI GCGC 1 cut(s) 216
GluI GCNGC 2 cut(s) 123, 528
HaeIII GGCC 1 cut(s) 538
HapII CCGG 3 cut(s) 150, 535, 551
HhaI GCGC 1 cut(s) 217
Hin1II CATG 3 cut(s) 139, 161, 320
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HindIII AAGCTT 1 cut(s) 321
HinfI GANTC 5 cut(s) 35, 60, 153, 219, 392
HpaII CCGG 3 cut(s) 150, 535, 551
HphI GGTGA 2 cut(s) 139, 394
Hpy166II GTNNAC 1 cut(s) 385
Hpy188I TCNGA 3 cut(s) 197, 579, 600
Hpy188III TCNNGA 4 cut(s) 53, 206, 310, 344
Hpy8I GTNNAC 1 cut(s) 385
HpyCH4III ACNGT 1 cut(s) 98
HpyCH4IV ACGT 1 cut(s) 438
HpyCH4V TGCA 3 cut(s) 301, 316, 449
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 491, 524
HpyF3I CTNAG 2 cut(s) 68, 126
HpySE526I ACGT 1 cut(s) 438
Hsp92II CATG 3 cut(s) 139, 161, 320
HspAI GCGC 1 cut(s) 215
Kzo9I GATC 2 cut(s) 252, 340
LmnI GCTCC 3 cut(s) 110, 198, 535
LpnPI CCDG 9 cut(s) 80, 163, 295, 340, 357, 504, 528, 548, 564
Lsp1109I GCAGC 1 cut(s) 539
LweI GCATC 2 cut(s) 16, 303
MaeI CTAG 3 cut(s) 290, 482, 591
MaeII ACGT 1 cut(s) 438
MaeIII GTNAC 2 cut(s) 546, 571
MalI GATC 2 cut(s) 254, 342
MboI GATC 2 cut(s) 252, 340
MflI RGATCY 2 cut(s) 252, 340
MhlI GDGCHC 1 cut(s) 195
MluCI AATT 4 cut(s) 417, 504, 510, 562
MlyI GAGTC 4 cut(s) 29, 162, 228, 401
MmeI TCCRAC 2 cut(s) 36, 497
Mph1103I ATGCAT 2 cut(s) 303, 318
MroXI GAANNNNTTC 1 cut(s) 237
MslI CAYNNNNRTG 1 cut(s) 380
MspA1I CMGCKG 1 cut(s) 125
MspI CCGG 3 cut(s) 150, 535, 551
MspR9I CCNGG 2 cut(s) 535, 552
MvnI CGCG 3 cut(s) 92, 217, 231
MwoI GCNNNNNNNGC 3 cut(s) 119, 491, 524
NciI CCSGG 2 cut(s) 535, 552
NdeII GATC 2 cut(s) 252, 340
NlaIII CATG 3 cut(s) 139, 161, 320
NlaIV GGNNCC 3 cut(s) 112, 342, 555
NsiI ATGCAT 2 cut(s) 303, 318
PaeR7I CTCGAG 1 cut(s) 522
PdmI GAANNNNTTC 1 cut(s) 237
PfeI GAWTC 1 cut(s) 60
PflMI CCANNNNNTGG 1 cut(s) 268
PkrI GCNGC 2 cut(s) 124, 529
PleI GAGTC 4 cut(s) 29, 161, 227, 400
PpsI GAGTC 4 cut(s) 29, 161, 227, 400
PpuMI RGGWCCY 1 cut(s) 603
Psp124BI GAGCTC 1 cut(s) 195
Psp5II RGGWCCY 1 cut(s) 603
PspN4I GGNNCC 3 cut(s) 112, 342, 555
PspPI GGNCC 2 cut(s) 554, 603
PspPPI RGGWCCY 1 cut(s) 603
PspXI VCTCGAGB 1 cut(s) 522
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 2 cut(s) 252, 340
RsaI GTAC 3 cut(s) 89, 350, 459
RsaNI GTAC 3 cut(s) 88, 349, 458
RseI CAYNNNNRTG 1 cut(s) 380
SacI GAGCTC 1 cut(s) 195
SatI GCNGC 2 cut(s) 123, 528
Sau3AI GATC 2 cut(s) 252, 340
Sau96I GGNCC 2 cut(s) 554, 603
SchI GAGTC 4 cut(s) 29, 162, 228, 401
ScrFI CCNGG 2 cut(s) 535, 552
SduI GDGCHC 1 cut(s) 195
SfaNI GCATC 2 cut(s) 16, 303
Sfr274I CTCGAG 1 cut(s) 522
SinI GGWCC 2 cut(s) 554, 603
SlaI CTCGAG 1 cut(s) 522
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 2 cut(s) 465, 522
SmoI CTYRAG 2 cut(s) 465, 522
Sse9I AATT 4 cut(s) 417, 504, 510, 562
SsiI CCGC 4 cut(s) 78, 92, 123, 229
SspMI CTAG 3 cut(s) 290, 482, 591
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 2 cut(s) 533, 550
TaaI ACNGT 1 cut(s) 98
TaiI ACGT 1 cut(s) 441
TaqI TCGA 2 cut(s) 54, 523
TaqII GACCGA 1 cut(s) 571
TasI AATT 4 cut(s) 417, 504, 510, 562
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 1 cut(s) 60
TseI GCWGC 1 cut(s) 527
TspDTI ATGAA 2 cut(s) 333, 364
Van91I CCANNNNNTGG 1 cut(s) 268
VpaK11BI GGWCC 2 cut(s) 554, 603
XapI RAATTY 2 cut(s) 417, 562
XcmI CCANNNNNNNNNTGG 1 cut(s) 269
XhoI CTCGAG 1 cut(s) 522
XmnI GAANNNNTTC 1 cut(s) 237
XspI CTAG 3 cut(s) 290, 482, 591
Zsp2I ATGCAT 2 cut(s) 303, 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.