Rmu_sc0005127.1_g000005

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005127.1
OG0004795
Physical Location & Seq
Forward (+)
21429 .. 34102
12674 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4851 bp
atggacgacgtgccgcccactacgggtcctccaccgccgcagaggggtggggaagaggagggtcgggctggggagtcgggtgatggagctccgaggccggataaggatccgaggaaggttgcccggaggtatcaattggagctgtgtcagaaagctgtggaggagaacataatcgtctatttggaaactggctgtggaaagacccacatagcagtgttgcttatctatgagctgcgtgagttgataatgaaacctcataagaacatatgtgtgtttcttgcgcctacggtggcgcttgttcaacagcaagcaagagttatagaagattcccttgatctcaaggttgggacctattgtggaagctctaagaaattgaaaactcaccaagactgggaaaaagagatggaacaatatgaggtttttgtcatgacgcctcaaatactgttacgtaacttataccaccgtattattaagatggagttgattgcattgctgatttttgatgagtgtcatcacgctcaaataaaaagcaaccatccttatgcagaaattatgaaattttacaagaatgatgccacgaaacttcctcgtatatttggcatgactgcatctccagttgtaggtaaaggcacttctagtcaatcgaatttatcaaagagtatcaatagtcttgaaaatttacttgatgctaaggtttatacagttgaagacaagacagaattgcattgttctgttgtttctcctgttatcaacgtccatcgttatggtcctgttattagtggcacatccagcccctgcatgcctttttctagaaaacttgacctgctgaaacgccagtgtatagcggaacttagtaagaagacgagtgacttccaaagtctaaggagtacaaagaaaacattaaacagagtgcatgacagtgtaatattttgcttggaaactcttggcctctggggtgcgttacaagctaggcatattctttccagtggtgataactttgaacgaaatgaattaatagaggaaggcaataatgatatagaggcagaaggaaataatagtgatgactctgtatgtgcggaatatctggctcaggcagctgacatcattcgtactgactatgtgaaagatgctgttgcatctgggctatctcgtgtagagatcttaaaggagccattcttctcaagtaaggtcttacgtcttattggaattctttcatccttcaggttgcagcaggatatgaaatgcataatttttgtcaataggattattactgcaagatccttgtcctacattctacaaaaccttaagaatctagcatcttggaagtgcgattttcttgtaggagtccactcgaaactgaagagtatgtcaaggaagacgatgcagatcattcttgataagtttcggtctggagagttgaatctattggttgcaactaaagttggtgaagagggactagatattcagacttgctgcctcgtgatacgatttgatcttccagaaactgttgctagctttatacagtcacgaggtcgtgcacggatgcctcagtctgaatatatatttttggtaaacagtggtagtcagaaagagttggatttaatagaaaacttcaggaaagatgaagagcgaatgaacatggaagttgctttcagaacatccagtgagacttttcttagtcctgaggaacgaacatataaagttgcttcttctggagcttccgtcacttctggatatagcatctcattgttacacctgtattgttcgaagcttccacatgatgatacaccacaatcttcgatggaagatgccaaaaaagatgcatgtctaaaagccatcgaagaattgcataaattgggtgcattaagtgactatctcttgccacagcaagacaatgcaaatgtggaggagtccatgctagactcttctgattatgacagcatggaagatgaagacttacgagaggaactacatgaaatgctggttcctgctgtcctaaaagagtcatggaataagttggaggatctcgtcaccttcagcgcttactattttcaatttgatcctgaacctaatgatagaatctatagaagttttggtctatttgtcaaagcacctcttccggcagaggccgaaacaatggaacttgatcttcacctcgctcatggtagatctgtgatgacaaagttggtgccatcaggatttgcagaatttgtcaaagatgagatcttgctggctcaaaactttcaagaaatgttcctcaagctcattctggaccgatcagaatttgtttctgaatttgttcccctgggaaactatgattttagcagatcgagcacatcaaccttctacctattgcttccagtaactttgggtgaaaatgataagataaacatagattggaggattatcaaaaaatgtttatcatctccaatattcaggggtccaggagatgctatggacagtaaaattatatcttcgggtattcgacttgcatctggttatagaagcatcagtgaagttgaggatagtttagtttatgtcccatataagaatacattttacttcatcactaatgttactcgcgaaagaaatgcctacagccaatataaagaaaagtcagatgctttaacctatgtggatcatctaagtaaaaagtttgacatttatcttaaataccctgagcaacatcttttgtgtgcaaaaccagttttcaatttgcacaacttgctccacaatcgtaggcaagaagattcagaagcacagcaactagatgagtattatatatatgtgcctcctgagctttgcgagctgaaggtaattggcttttcaaaagatattgggagttcggtatctctgttaccatcaatcatgcaccgtctggaaaacttgcttgtcgccatcgaactgaagcatgtattatgcacttcattccccgaaggagctgaggttacttcgcagagagttctagaagccctcacaactgagaagtgccaggagcggatttcccttgaaaggcttgaaatactaggagatgctttcctcaaatttgcagttggaaggcactttttccttttgcatgcttcacttgatgaaggtgaactaacaaggaaacgttcaaatgtggtaaacaattccaatttattgaagctagctactagaagcaacttacaggtatacatacgggatcagccatttgaaccctctcagttcttcactttaggccgcccttgcaaaaatatttgtgaccaggagactataggatctcttgattctcaagatttaggcagtgcagtaaaacagtcacatgctcgtcacgtcagatgtagtaagggtcaccattggttacataagaaaacaattgctgatgttgttgaagcccttgttggagcattcatagttgacagcggttttaaagcggcaactgcatttctcagatggattggcataaaagtaaactttgaagcatcagaagttactaaagtatgcatagcaagcagcagatacatttcacttgctgcttgcatagacattgcggcccttgaaaattcattagggtatcagtttctccacagaggtttgctgttacaggcatttgtacatccttcttacaacaaacatggaggaggttgctatcagagattggagtttcttggagatgctgtcttggattacttgatcacctcatacctgtattcagtgtatccaaagttaaagcctggccatttgacagatttgagatcaattgctgtgaataataaagcctttgctactgttgcagtagctcgatccttccacaagtttctactctctgacaccagtaccctttctgaggctataaagacttatgtgaacttcattgagacatctgcatcagatagcagtctggttgatgggccaaaatgccccaaggctctgggcgacttggtagagtcttgtcttggtgcaattcttcttgatacagggttcgacttaaaccttgtttggaagataatgctatcctttctggagcctgtcatgagcttttccaacattcagcttagtcctgttagggaactacgggaactttgccaagctcatgcatgggttcttaagtttttaccatcaaagaagggtaaaacattttcagttgaggcaacagtgaatgggaataatgtttctgcaactgcttcctcaactgccctgaacaaaagggaggctattagaatttcggctcagttaattttcgaaacgttgaaggctcaaggcaatattccaaagtctaagttgtctttggaggaagtcttgaagtcaagctgcaagttggaacccaaacttatcggatatgatgaaacaccaatagatgttacagctcttgatgttattggatttgataagttgaatgtacaagaggcttctagcagcagttccaactcagatgtgcattctatgagtgaagtcagcagtggtaactcctcctgcataaaacgtttacgacaacccccagcttcacttggagaagtcaaaatggacagcactaatgcctgtaacagttgtagctctgatgctatctcacaaaccagaggtgtgccacacaaattaacagcaaaatctcgtttgcatgagacctgtgttgctaacttctgggaaccccctgaatttgaatgttgcaaagaggaagggccaggccacataaaatcgttcactttcaaggttacagtgaagatagatgatgtttcggatacacttttggaggcctttggagaacctcgtacgagcaagaaagcagcagcagagcatgcagcggaaggggcactttggtacttggaaaagaaaggttacatagaggagagtgatcaatcagaggaaccttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006351 GO:0006353 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009798 GO:0009888 GO:0009892 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010050 GO:0010073 GO:0010074 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010599 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048507 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065001 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0097659 GO:0098542 GO:0098586 GO:0098727 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1616

Amino Acids

181.17

Weight (kDa)

5.91

Isoelectric Point (pI)

49.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 42 - 205 3e-17 Type III restriction enzyme, res subunit
DEAD PF00270 43 - 205 1.6e-18 DEAD/DEAH box helicase
Helicase_C PF00271 400 - 513 8.8e-18 Helicase conserved C-terminal domain
Dicer_dimer PF03368 597 - 631 2.8e-06 Dicer dimerisation domain
PAZ PF02170 839 - 959 3.2e-09 PAZ domain
Ribonucleas_3_3 PF14622 1003 - 1061 5.8e-07 Ribonuclease-III-like
Ribonuclease_3 PF00636 1004 - 1136 3.2e-18 Ribonuclease III domain
Ribonucleas_3_3 PF14622 1192 - 1323 7.6e-14 Ribonuclease-III-like
Ribonuclease_3 PF00636 1212 - 1321 3.5e-21 Ribonuclease III domain
DND1_DSRM PF14709 1527 - 1602 4.9e-14 double strand RNA binding domain from DEAD END PROTEIN 1
dsrm PF00035 1528 - 1598 1.4e-06 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 831
AccB1I GGYRCC 1 cut(s) 2188
AccBSI CCGCTC 1 cut(s) 2998
AccI GTMKAC 1 cut(s) 3174
AccII CGCG 1 cut(s) 2583
AclI AACGTT 3 cut(s) 3112, 4229, 4465
AclWI GGATC 9 cut(s) 101, 114, 1272, 2031, 2054, 2646, 3192, 3268, 3780
AcoI YGGCCR 1 cut(s) 3718
AcuI CTGAAG 6 cut(s) 1204, 1379, 1597, 2020, 2831, 2927
AcyI GRCGYC 1 cut(s) 431
AfaI GTAC 7 cut(s) 889, 1111, 3597, 3820, 4383, 4750, 4799
AfeI AGCGCT 1 cut(s) 2041
AfiI CCNNNNNNNGG 6 cut(s) 23, 44, 391, 620, 3012, 3829
AflII CTTAAG 2 cut(s) 1304, 4088
AjiI CACGTC 2 cut(s) 10, 3316
AjnI CCWGG 6 cut(s) 2304, 2443, 2991, 3246, 3715, 4660
AjuI GAANNNNNNNTTGG 2 cut(s) 3366, 3398
Alw21I GWGCWC 3 cut(s) 91, 1540, 2336
Alw26I GTCTC 4 cut(s) 1661, 3245, 3854, 4595
Alw44I GTGCAC 1 cut(s) 1536
AlwI GGATC 9 cut(s) 101, 114, 1272, 2031, 2054, 2646, 3192, 3268, 3780
AlwNI CAGNNNCTG 2 cut(s) 795, 1505
Aor51HI AGCGCT 1 cut(s) 2041
ApaLI GTGCAC 1 cut(s) 1536
AseI ATTAAT 1 cut(s) 1013
Asp700I GAANNNNTTC 2 cut(s) 1210, 2298
AspLEI GCGC 3 cut(s) 283, 295, 2042
AspS9I GGNCC 8 cut(s) 26, 348, 767, 2272, 2441, 3535, 3893, 4658
AsuC2I CCSGG 1 cut(s) 124
AsuII TTCGAA 2 cut(s) 1766, 4224
AsuNHI GCTAGC 2 cut(s) 1511, 3148
AvaII GGWCC 5 cut(s) 26, 348, 767, 2272, 2441
AxyI CCTNAGG 1 cut(s) 1683
BaeGI GKGCMC 2 cut(s) 1540, 4792
BaeI ACNNNNGTAYC 2 cut(s) 3802, 3835
BalI TGGCCA 1 cut(s) 3720
BamHI GGATCC 1 cut(s) 106
BanI GGYRCC 1 cut(s) 2188
BanII GRGCYC 1 cut(s) 91
BarI GAAGNNNNNNTAC 2 cut(s) 616, 648
BauI CACGAG 3 cut(s) 1149, 1478, 1527
BbsI GAAGAC 4 cut(s) 714, 866, 1382, 1959
Bbv12I GWGCWC 3 cut(s) 91, 1540, 2336
BbvCI CCTCAGC 1 cut(s) 2943
BcgI CGANNNNNNTGC 2 cut(s) 2573, 2607
BciT130I CCWGG 6 cut(s) 2306, 2445, 2993, 3248, 3717, 4662
BciVI GTATCC 2 cut(s) 3711, 4711
BclI TGATCA 2 cut(s) 3675, 4831
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 4 cut(s) 1661, 3245, 3854, 4595
BfmI CTRYAG 3 cut(s) 2083, 2596, 3255
BfoI RGCGCY 2 cut(s) 296, 2043
BfrI CTTAAG 2 cut(s) 1304, 4088
BfuAI ACCTGC 1 cut(s) 831
BfuI GTATCC 2 cut(s) 3711, 4711
BglII AGATCT 3 cut(s) 1158, 2168, 2223
Bme1390I CCNGG 7 cut(s) 124, 2306, 2445, 2993, 3248, 3717, 4662
Bme18I GGWCC 5 cut(s) 26, 348, 767, 2272, 2441
BmgBI CACGTC 2 cut(s) 10, 3316
BmgT120I GGNCC 8 cut(s) 26, 348, 767, 2272, 2441, 3535, 3893, 4658
BmrFI CCNGG 7 cut(s) 124, 2306, 2445, 2993, 3248, 3717, 4662
BmrI ACTGGG 1 cut(s) 400
BmtI GCTAGC 2 cut(s) 1515, 3152
BmuI ACTGGG 1 cut(s) 400
BpiI GAAGAC 4 cut(s) 714, 866, 1382, 1959
BpmI CTGGAG 4 cut(s) 597, 1431, 1734, 4025
Bpu10I CCTNAGC 5 cut(s) 690, 1089, 2679, 2796, 2943
Bpu14I TTCGAA 2 cut(s) 1766, 4224
BpuEI CTTGAG 5 cut(s) 323, 1165, 2243, 3258, 4224
BpuMI CCSGG 1 cut(s) 124
BsaAI YACGTR 1 cut(s) 449
BsaBI GATNNNNATC 2 cut(s) 105, 1385
BsaHI GRCGYC 1 cut(s) 431
BsaI GGTCTC 1 cut(s) 4595
BsaJI CCNNGG 5 cut(s) 92, 110, 2304, 2305, 3906
Bsc4I CCNNNNNNNGG 6 cut(s) 23, 44, 391, 620, 3012, 3829
Bse1I ACTGG 9 cut(s) 193, 395, 614, 835, 984, 1662, 2360, 2705, 3816
Bse21I CCTNAGG 1 cut(s) 1683
Bse3DI GCAATG 2 cut(s) 488, 3528
Bse8I GATNNNNATC 2 cut(s) 105, 1385
BseBI CCWGG 6 cut(s) 2306, 2445, 2993, 3248, 3717, 4662
BseDI CCNNGG 5 cut(s) 92, 110, 2304, 2305, 3906
BseGI GGATG 6 cut(s) 535, 785, 1214, 1548, 1658, 3598
BseJI GATNNNNATC 2 cut(s) 105, 1385
BseLI CCNNNNNNNGG 6 cut(s) 23, 44, 391, 620, 3012, 3829
BseMI GCAATG 2 cut(s) 488, 3528
BseNI ACTGG 9 cut(s) 193, 395, 614, 835, 984, 1662, 2360, 2705, 3816
BseRI GAGGAG 6 cut(s) 71, 176, 1922, 3636, 4441, 4838
BseSI GKGCMC 2 cut(s) 1540, 4792
BseYI CCCAGC 2 cut(s) 68, 4480
BsgI GTGCAG 1 cut(s) 3309
Bsh1236I CGCG 1 cut(s) 2583
BshNI GGYRCC 1 cut(s) 2188
BsiHKAI GWGCWC 3 cut(s) 91, 1540, 2336
BsiSI CCGG 3 cut(s) 98, 124, 2120
BsiWI CGTACG 1 cut(s) 4748
BslFI GGGAC 3 cut(s) 361, 1467, 2525
BslI CCNNNNNNNGG 6 cut(s) 23, 44, 391, 620, 3012, 3829
BsmAI GTCTC 4 cut(s) 1661, 3245, 3854, 4595
BsmFI GGGAC 3 cut(s) 361, 1467, 2525
BsmI GAATGC 2 cut(s) 3389, 4420
Bso31I GGTCTC 1 cut(s) 4595
Bsp119I TTCGAA 2 cut(s) 1766, 4224
Bsp1286I GDGCHC 4 cut(s) 91, 1540, 2336, 4792
Bsp1407I TGTACA 2 cut(s) 3595, 4381
Bsp68I TCGCGA 1 cut(s) 2583
BspFNI CGCG 1 cut(s) 2583
BspHI TCATGA 2 cut(s) 426, 4014
BspMI ACCTGC 1 cut(s) 831
BspOI GCTAGC 2 cut(s) 1515, 3152
BspPI GGATC 9 cut(s) 101, 114, 1272, 2031, 2054, 2646, 3192, 3268, 3780
BspQI GCTCTTC 1 cut(s) 1620
BspT104I TTCGAA 2 cut(s) 1766, 4224
BspT107I GGYRCC 1 cut(s) 2188
BspTI CTTAAG 2 cut(s) 1304, 4088
BspTNI GGTCTC 1 cut(s) 4595
BsrBI CCGCTC 1 cut(s) 2998
BsrDI GCAATG 2 cut(s) 488, 3528
BsrGI TGTACA 2 cut(s) 3595, 4381
BsrI ACTGG 9 cut(s) 193, 395, 614, 835, 984, 1662, 2360, 2705, 3816
BssECI CCNNGG 5 cut(s) 92, 110, 2304, 2305, 3906
BssNAI GTATAC 1 cut(s) 3175
BssNI GRCGYC 1 cut(s) 431
BssSI CACGAG 3 cut(s) 1149, 1478, 1527
BssT1I CCWWGG 1 cut(s) 3906
Bst1107I GTATAC 1 cut(s) 3175
Bst2BI CACGAG 3 cut(s) 1149, 1478, 1527
Bst2UI CCWGG 6 cut(s) 2306, 2445, 2993, 3248, 3717, 4662
Bst6I CTCTTC 6 cut(s) 48, 1355, 1443, 1620, 1930, 2121
BstACI GRCGYC 1 cut(s) 431
BstAFI CTTAAG 2 cut(s) 1304, 4088
BstAPI GCANNNNNTGC 2 cut(s) 2725, 4775
BstAUI TGTACA 2 cut(s) 3595, 4381
BstBAI YACGTR 1 cut(s) 449
BstBI TTCGAA 2 cut(s) 1766, 4224
BstEII GGTNACC 1 cut(s) 3332
BstENI CCTNNNNNAGG 1 cut(s) 3827
BstF5I GGATG 6 cut(s) 535, 785, 1214, 1548, 1658, 3598
BstFNI CGCG 1 cut(s) 2583
BstH2I RGCGCY 2 cut(s) 296, 2043
BstHHI GCGC 3 cut(s) 283, 295, 2042
BstMAI GTCTC 4 cut(s) 1661, 3245, 3854, 4595
BstNI CCWGG 6 cut(s) 2306, 2445, 2993, 3248, 3717, 4662
BstNSI RCATGY 6 cut(s) 802, 1827, 2915, 3080, 3308, 4778
BstPI GGTNACC 1 cut(s) 3332
BstSCI CCNGG 7 cut(s) 122, 2304, 2443, 2991, 3246, 3715, 4660
BstSFI CTRYAG 3 cut(s) 2083, 2596, 3255
BstSLI GKGCMC 2 cut(s) 1540, 4792
BstSNI TACGTA 1 cut(s) 449
BstUI CGCG 1 cut(s) 2583
BstV2I GAAGAC 4 cut(s) 714, 866, 1382, 1959
BstX2I RGATCY 7 cut(s) 106, 1158, 1277, 2023, 2168, 2223, 3260
BstXI CCANNNNNNTGG 2 cut(s) 764, 3913
BstYI RGATCY 7 cut(s) 106, 1158, 1277, 2023, 2168, 2223, 3260
BstZ17I GTATAC 1 cut(s) 3175
Bsu36I CCTNAGG 1 cut(s) 1683
BsuI GTATCC 2 cut(s) 3711, 4711
BtrI CACGTC 2 cut(s) 10, 3316
BtsCI GGATG 6 cut(s) 535, 785, 1214, 1548, 1658, 3598
BtsI GCAGTG 3 cut(s) 219, 3292, 4447
BtuMI TCGCGA 1 cut(s) 2583
BveI ACCTGC 1 cut(s) 831
CaiI CAGNNNCTG 2 cut(s) 795, 1505
CciI TCATGA 2 cut(s) 426, 4014
CfoI GCGC 3 cut(s) 283, 295, 2042
Cfr13I GGNCC 8 cut(s) 26, 348, 767, 2272, 2441, 3535, 3893, 4658
CseI GACGC 1 cut(s) 439
Csp6I GTAC 7 cut(s) 888, 1110, 3596, 3819, 4382, 4749, 4798
CviQI GTAC 7 cut(s) 888, 1110, 3596, 3819, 4382, 4749, 4798
DraI TTTAAA 1 cut(s) 3412
EaeI YGGCCR 1 cut(s) 3718
Eam1104I CTCTTC 6 cut(s) 48, 1355, 1443, 1620, 1930, 2121
EarI CTCTTC 6 cut(s) 48, 1355, 1443, 1620, 1930, 2121
Ecl136II GAGCTC 1 cut(s) 89
Eco105I TACGTA 1 cut(s) 449
Eco130I CCWWGG 1 cut(s) 3906
Eco147I AGGCCT 1 cut(s) 4733
Eco24I GRGCYC 1 cut(s) 91
Eco31I GGTCTC 1 cut(s) 4595
Eco47I GGWCC 5 cut(s) 26, 348, 767, 2272, 2441
Eco47III AGCGCT 1 cut(s) 2041
Eco53kI GAGCTC 1 cut(s) 89
Eco57I CTGAAG 6 cut(s) 1204, 1379, 1597, 2020, 2831, 2927
Eco81I CCTNAGG 1 cut(s) 1683
Eco91I GGTNACC 1 cut(s) 3332
EcoICRI GAGCTC 1 cut(s) 89
EcoNI CCTNNNNNAGG 1 cut(s) 3827
EcoO109I RGGNCCY 2 cut(s) 26, 348
EcoO65I GGTNACC 1 cut(s) 3332
EcoRI GAATTC 1 cut(s) 1206
EcoRII CCWGG 6 cut(s) 2304, 2443, 2991, 3246, 3715, 4660
EcoT14I CCWWGG 1 cut(s) 3906
EcoT22I ATGCAT 4 cut(s) 1247, 1825, 3488, 4081
EcoT38I GRGCYC 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 3906
FalI AAGNNNNNCTT 6 cut(s) 315, 347, 2100, 2132, 4776, 4808
FaqI GGGAC 3 cut(s) 361, 1467, 2525
FauNDI CATATG 1 cut(s) 266
FbaI TGATCA 2 cut(s) 3675, 4831
FblI GTMKAC 1 cut(s) 3174
FokI GGATG 6 cut(s) 522, 772, 1201, 1555, 1645, 3585
FriOI GRGCYC 1 cut(s) 91
GlaI GCGC 3 cut(s) 282, 294, 2041
GsaI CCCAGC 2 cut(s) 72, 4484
GsuI CTGGAG 4 cut(s) 597, 1431, 1734, 4025
HaeII RGCGCY 2 cut(s) 296, 2043
HapII CCGG 3 cut(s) 98, 124, 2120
HgaI GACGC 1 cut(s) 439
HhaI GCGC 3 cut(s) 283, 295, 2042
Hin1I GRCGYC 1 cut(s) 431
Hin6I GCGC 3 cut(s) 281, 293, 2040
HinP1I GCGC 3 cut(s) 281, 293, 2040
HincII GTYRAC 1 cut(s) 3400
HindII GTYRAC 1 cut(s) 3400
HindIII AAGCTT 1 cut(s) 1769
HpaII CCGG 3 cut(s) 98, 124, 2120
Hpy99I CGWCG 1 cut(s) 11
HpyCH4IV ACGT 8 cut(s) 9, 448, 753, 1195, 3112, 3315, 4229, 4465
HpySE526I ACGT 8 cut(s) 9, 448, 753, 1195, 3112, 3315, 4229, 4465
Hsp92I GRCGYC 1 cut(s) 431
HspAI GCGC 3 cut(s) 281, 293, 2040
Ksp22I TGATCA 2 cut(s) 3675, 4831
LguI GCTCTTC 1 cut(s) 1620
MaeII ACGT 8 cut(s) 9, 448, 753, 1195, 3112, 3315, 4229, 4465
MbiI CCGCTC 1 cut(s) 2998
MfeI CAATTG 3 cut(s) 134, 3357, 3741
MflI RGATCY 7 cut(s) 106, 1158, 1277, 2023, 2168, 2223, 3260
MhlI GDGCHC 4 cut(s) 91, 1540, 2336, 4792
MlsI TGGCCA 1 cut(s) 3720
MluNI TGGCCA 1 cut(s) 3720
MlyI GAGTC 7 cut(s) 83, 1058, 1353, 1916, 1919, 2012, 3938
MmeI TCCRAC 7 cut(s) 1575, 1998, 3034, 3364, 4050, 4281, 4431
Mox20I TGGCCA 1 cut(s) 3720
Mph1103I ATGCAT 4 cut(s) 1247, 1825, 3488, 4081
MroXI GAANNNNTTC 2 cut(s) 1210, 2298
MscI TGGCCA 1 cut(s) 3720
MslI CAYNNNNRTG 5 cut(s) 212, 269, 540, 762, 918
Msp20I TGGCCA 1 cut(s) 3720
MspA1I CMGCKG 3 cut(s) 1097, 3405, 4781
MspCI CTTAAG 2 cut(s) 1304, 4088
MspI CCGG 3 cut(s) 98, 124, 2120
MspR9I CCNGG 7 cut(s) 124, 2306, 2445, 2993, 3248, 3717, 4662
MunI CAATTG 3 cut(s) 134, 3357, 3741
Mva1269I GAATGC 2 cut(s) 3389, 4420
MvaI CCWGG 6 cut(s) 2306, 2445, 2993, 3248, 3717, 4662
MvnI CGCG 1 cut(s) 2583
NciI CCSGG 1 cut(s) 124
NdeI CATATG 1 cut(s) 266
NheI GCTAGC 2 cut(s) 1511, 3148
NmuCI GTSAC 9 cut(s) 866, 1524, 1723, 1868, 2029, 3242, 3300, 3311, 3332
NruI TCGCGA 1 cut(s) 2583
NsiI ATGCAT 4 cut(s) 1247, 1825, 3488, 4081
NspI RCATGY 6 cut(s) 802, 1827, 2915, 3080, 3308, 4778
NspV TTCGAA 2 cut(s) 1766, 4224
PaeI GCATGC 3 cut(s) 802, 3080, 4778
PagI TCATGA 2 cut(s) 426, 4014
PasI CCCWGGG 1 cut(s) 2305
PceI AGGCCT 1 cut(s) 4733
PciSI GCTCTTC 1 cut(s) 1620
PctI GAATGC 2 cut(s) 3389, 4420
PdmI GAANNNNTTC 2 cut(s) 1210, 2298
PfeI GAWTC 6 cut(s) 326, 1309, 1420, 2079, 2750, 3269
Pfl23II CGTACG 1 cut(s) 4748
PfoI TCCNGGA 1 cut(s) 2443
PleI GAGTC 7 cut(s) 82, 1058, 1352, 1916, 1918, 2011, 3937
PpsI GAGTC 7 cut(s) 82, 1058, 1352, 1916, 1918, 2011, 3937
Ppu21I YACGTR 1 cut(s) 449
PpuMI RGGWCCY 2 cut(s) 26, 348
PshBI ATTAAT 1 cut(s) 1013
Psp124BI GAGCTC 1 cut(s) 91
Psp1406I AACGTT 3 cut(s) 3112, 4229, 4465
Psp5II RGGWCCY 2 cut(s) 26, 348
Psp6I CCWGG 6 cut(s) 2304, 2443, 2991, 3246, 3715, 4660
PspEI GGTNACC 1 cut(s) 3332
PspFI CCCAGC 2 cut(s) 68, 4480
PspGI CCWGG 6 cut(s) 2304, 2443, 2991, 3246, 3715, 4660
PspLI CGTACG 1 cut(s) 4748
PspPI GGNCC 8 cut(s) 26, 348, 767, 2272, 2441, 3535, 3893, 4658
PspPPI RGGWCCY 2 cut(s) 26, 348
PstNI CAGNNNCTG 2 cut(s) 795, 1505
PsuI RGATCY 7 cut(s) 106, 1158, 1277, 2023, 2168, 2223, 3260
PvuII CAGCTG 1 cut(s) 1097
RruI TCGCGA 1 cut(s) 2583
RsaI GTAC 7 cut(s) 889, 1111, 3597, 3820, 4383, 4750, 4799
RsaNI GTAC 7 cut(s) 888, 1110, 3596, 3819, 4382, 4749, 4798
RseI CAYNNNNRTG 5 cut(s) 212, 269, 540, 762, 918
SacI GAGCTC 1 cut(s) 91
SapI GCTCTTC 1 cut(s) 1620
Sau96I GGNCC 8 cut(s) 26, 348, 767, 2272, 2441, 3535, 3893, 4658
SchI GAGTC 7 cut(s) 83, 1058, 1353, 1916, 1919, 2012, 3938
ScrFI CCNGG 7 cut(s) 124, 2306, 2445, 2993, 3248, 3717, 4662
SduI GDGCHC 4 cut(s) 91, 1540, 2336, 4792
SfcI CTRYAG 3 cut(s) 2083, 2596, 3255
SfuI TTCGAA 2 cut(s) 1766, 4224
SinI GGWCC 5 cut(s) 26, 348, 767, 2272, 2441
SmiMI CAYNNNNRTG 5 cut(s) 212, 269, 540, 762, 918
SmlI CTYRAG 7 cut(s) 338, 1180, 1304, 2258, 3273, 4088, 4239
SmoI CTYRAG 7 cut(s) 338, 1180, 1304, 2258, 3273, 4088, 4239
SnaBI TACGTA 1 cut(s) 449
SphI GCATGC 3 cut(s) 802, 3080, 4778
SseBI AGGCCT 1 cut(s) 4733
SspI AATATT 4 cut(s) 927, 2433, 3238, 4249
SstI GAGCTC 1 cut(s) 91
StuI AGGCCT 1 cut(s) 4733
StyD4I CCNGG 7 cut(s) 122, 2304, 2443, 2991, 3246, 3715, 4660
StyI CCWWGG 1 cut(s) 3906
TaiI ACGT 8 cut(s) 12, 451, 756, 1198, 3115, 3318, 4232, 4468
TaqII GACCGA 2 cut(s) 1395, 2289
TatI WGTACW 3 cut(s) 887, 3595, 4381
TauI GCSGC 5 cut(s) 16, 40, 3225, 3419, 3536
TfiI GAWTC 6 cut(s) 326, 1309, 1420, 2079, 2750, 3269
TseFI GTSAC 9 cut(s) 866, 1524, 1723, 1868, 2029, 3242, 3300, 3311, 3332
Tsp45I GTSAC 9 cut(s) 866, 1524, 1723, 1868, 2029, 3242, 3300, 3311, 3332
TspGWI ACGGA 2 cut(s) 1555, 1711
Vha464I CTTAAG 2 cut(s) 1304, 4088
VneI GTGCAC 1 cut(s) 1536
VpaK11BI GGWCC 5 cut(s) 26, 348, 767, 2272, 2441
VspI ATTAAT 1 cut(s) 1013
XagI CCTNNNNNAGG 1 cut(s) 3827
XbaI TCTAGA 2 cut(s) 809, 2965
XceI RCATGY 6 cut(s) 802, 1827, 2915, 3080, 3308, 4778
XmiI GTMKAC 1 cut(s) 3174
XmnI GAANNNNTTC 2 cut(s) 1210, 2298
Zsp2I ATGCAT 4 cut(s) 1247, 1825, 3488, 4081
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.