Rmu_sc0005363.1_g000051

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005363.1
OG0004012
Physical Location & Seq
Reverse (-)
191204 .. 194541
3338 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1941 bp
atgatcaatgacaccctcaacactgctgcaaagcttcagatggccatgattgtgacagaagtttacctctcggggcttccgaaggacactccatttcaaaattttgaacttaggtttaaggagtggggctttgagaagggttggggagacacagcagagagatccaaggaaacaatgagaatactatcagaagtactacaagctccagatccacttaacatggagaggtttttgagcaggcttcctaccatattcaatgttgtaatattctctcctcatggctattttggccaagctgatgttcttggtttgccagacaccggtggacaggttgtttatattcttgatcaagttaaagctctggaagaagaattgctccttagaattaagcaacaagggcttgctgtgaagcctcagatttttgtggtaacaagactcattcctgaagcaaggggaactacgtgcaatcaggagttggaaccgatcaatggcaccaagtactcaaacattcttagggttccatttaggacagaaaagggggtccttcgtcgttgggtttctcgttttgatatctacccctatcttgagctgtttgtgcaggatgctacagccaaaatcctcgacctcatggacgggaagcctgatcttattattggaaattacactgatgggaatttggtggcctctctgatggctaacaaacttggaataactcaggcaacaattgcacatgctttggagaagaccaagtatgaagactcagacatcaagtggaaggaattagaccctaagtatcacttctcatgccaatttctagcagacacaatttcaatgaatgcaacagattttgtcattgcaagcacattccaggaaattgcaggaagccaagacagaccaggacaatatgaaagccataccgcatttactctcccagggctttgtagagttgtttcaggcatcaatgtattcgatcccaaatttaacattgctgcacctggggctgaccagtctgtctattttccttacactgagaaacaaaaaagagtctcatcatttcatcctgccattgaagaactactgtatagcaaggaggataacaatgaacatgtggggtttctagtggataggaagaaacctatcatcttctcaatggcaaggcttgacactgttaagaacattactggtttagttgaatggtatggcaaaaacaagaggctcagaaatctggttaaccttgtagtagttggaggcttctttgacccttcaaaatcaaaagatagagaggaaatagcagaaattaaaaagatgcattcactgatagagaagtaccagctccagggtcagatcagatggatagcagcacagactgataggaaccgcaatggagaactctaccgttgtattgcggacaccaggggagcttttgtccaacctgctctatatgaagcttttggtcttactgtcattgaggcaatgaactgtggtttacccacttttgcaaccaaccaaggagggccagctgaaatcattgttgatgggatctctggtttccatattgatcccaacaatggtgatgatgcaagcagcaaaattgccgatttctttgagaaatgcaaaactgaggctacatactgggacaattattcaaaagcgggtttgcaacgcatttatgaatgctacacttggaagatatatgcaaacaaggtgttgaacatgggctgcacttatacattctggaggcagttgaacaaagagcagaaacaggcaaagcaaagatacatccagatgttctttaatctccaatatagaaatttggtgaagaatgtgcctatcccaagtgacgagcctgaaaaaccagcaccaaagccagtcaccaaatcacagacgactctaagcactagacgctcacagtcccgattgcaaaggttgtttggagcctga

Protein Analysis

646

Amino Acids

73.5

Weight (kDa)

8.18

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUS_EPBD PF24862 1 - 50 2.3e-14 Sucrose synthase EPBD domain
GT-B_Sucrose_synth PF00862 73 - 361 2e-170 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 373 - 544 1.1e-30 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 379 - 534 3.7e-13 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1010
Acc36I ACCTGC 1 cut(s) 1441
AccB1I GGYRCC 1 cut(s) 491
AciI CCGC 4 cut(s) 918, 1378, 1406, 1652
AclWI GGATC 5 cut(s) 156, 203, 965, 1547, 1553
AcoI YGGCCR 2 cut(s) 42, 289
AcsI RAATTY 4 cut(s) 100, 673, 977, 1810
AcuI CTGAAG 2 cut(s) 20, 465
AfaI GTAC 3 cut(s) 195, 500, 1328
AfiI CCNNNNNNNGG 4 cut(s) 320, 488, 1336, 1568
AflIII ACRYGT 1 cut(s) 1105
AgeI ACCGGT 1 cut(s) 320
AjnI CCWGG 6 cut(s) 867, 895, 931, 994, 1335, 1412
AjuI GAANNNNNNNTTGG 2 cut(s) 285, 317
AluBI AGCT 9 cut(s) 34, 203, 296, 359, 589, 1333, 1421, 1448, 1520
AluI AGCT 9 cut(s) 34, 203, 296, 359, 589, 1333, 1421, 1448, 1520
Alw26I GTCTC 2 cut(s) 141, 1051
AlwI GGATC 5 cut(s) 156, 203, 965, 1547, 1553
Ama87I CYCGRG 1 cut(s) 70
AoxI GGCC 4 cut(s) 42, 289, 681, 1514
ApeKI GCWGC 5 cut(s) 26, 989, 1358, 1584, 1719
ApoI RAATTY 4 cut(s) 100, 673, 977, 1810
AsiGI ACCGGT 1 cut(s) 320
AspS9I GGNCC 2 cut(s) 541, 1514
AsuHPI GGTGA 3 cut(s) 1583, 1828, 1864
AvaI CYCGRG 1 cut(s) 70
AvaII GGWCC 1 cut(s) 541
BalI TGGCCA 2 cut(s) 44, 291
BanI GGYRCC 1 cut(s) 491
BbsI GAAGAC 2 cut(s) 749, 762
BbvI GCAGC 5 cut(s) 13, 976, 1370, 1596, 1706
BccI CCATC 5 cut(s) 34, 662, 685, 1344, 1529
BciT130I CCWGG 6 cut(s) 869, 897, 933, 996, 1337, 1414
BclI TGATCA 2 cut(s) 3, 346
BcoDI GTCTC 2 cut(s) 141, 1051
BfaI CTAG 3 cut(s) 815, 1118, 1899
BfmI CTRYAG 1 cut(s) 606
BfuAI ACCTGC 1 cut(s) 1441
BisI GCNGC 5 cut(s) 27, 990, 1359, 1585, 1720
BlsI GCNGC 5 cut(s) 28, 991, 1360, 1586, 1721
BmcAI AGTACT 2 cut(s) 195, 500
Bme1390I CCNGG 6 cut(s) 869, 897, 933, 996, 1337, 1414
Bme18I GGWCC 1 cut(s) 541
BmeT110I CYCGRG 1 cut(s) 70
BmgT120I GGNCC 2 cut(s) 541, 1514
BmiI GGNNCC 6 cut(s) 480, 493, 519, 542, 1376, 1936
BmrFI CCNGG 6 cut(s) 869, 897, 933, 996, 1337, 1414
BmrI ACTGGG 1 cut(s) 1642
BmsI GCATC 4 cut(s) 592, 966, 1296, 1567
BmuI ACTGGG 1 cut(s) 1642
BpiI GAAGAC 2 cut(s) 749, 762
BpmI CTGGAG 3 cut(s) 189, 1319, 1756
BpuEI CTTGAG 1 cut(s) 605
BsaAI YACGTR 1 cut(s) 462
BsaJI CCNNGG 7 cut(s) 165, 931, 932, 995, 1336, 1413, 1507
BsaWI WCCGGW 1 cut(s) 320
BsaXI ACNNNNNCTCC 2 cut(s) 1410, 1440
Bsc4I CCNNNNNNNGG 4 cut(s) 320, 488, 1336, 1568
Bse118I RCCGGY 1 cut(s) 320
Bse1I ACTGG 4 cut(s) 1006, 1186, 1637, 1868
Bse3DI GCAATG 4 cut(s) 852, 984, 1387, 1479
BseBI CCWGG 6 cut(s) 869, 897, 933, 996, 1337, 1414
BseDI CCNNGG 7 cut(s) 165, 931, 932, 995, 1336, 1413, 1507
BseGI GGATG 3 cut(s) 607, 1057, 1779
BseLI CCNNNNNNNGG 4 cut(s) 320, 488, 1336, 1568
BseMI GCAATG 4 cut(s) 852, 984, 1387, 1479
BseMII CTCAG 6 cut(s) 428, 728, 774, 1020, 1231, 1611
BseNI ACTGG 4 cut(s) 1006, 1186, 1637, 1868
BseRI GAGGAG 1 cut(s) 264
BseXI GCAGC 5 cut(s) 13, 976, 1370, 1596, 1706
BsgI GTGCAG 3 cut(s) 617, 975, 1705
BshFI GGCC 4 cut(s) 44, 291, 683, 1516
BshNI GGYRCC 1 cut(s) 491
BshTI ACCGGT 1 cut(s) 320
BsiHKCI CYCGRG 1 cut(s) 70
BsiSI CCGG 1 cut(s) 321
BslFI GGGAC 2 cut(s) 1649, 1897
BslI CCNNNNNNNGG 4 cut(s) 320, 488, 1336, 1568
BsmAI GTCTC 2 cut(s) 141, 1051
BsmFI GGGAC 2 cut(s) 1649, 1897
BsmI GAATGC 3 cut(s) 841, 1309, 1679
BsnI GGCC 4 cut(s) 44, 291, 683, 1516
BsoBI CYCGRG 1 cut(s) 70
BspACI CCGC 4 cut(s) 918, 1378, 1406, 1652
BspANI GGCC 4 cut(s) 44, 291, 683, 1516
BspCNI CTCAG 6 cut(s) 427, 727, 773, 1021, 1230, 1612
BspLI GGNNCC 6 cut(s) 480, 493, 519, 542, 1376, 1936
BspMI ACCTGC 1 cut(s) 1441
BspPI GGATC 5 cut(s) 156, 203, 965, 1547, 1553
BspT107I GGYRCC 1 cut(s) 491
BsrDI GCAATG 4 cut(s) 852, 984, 1387, 1479
BsrFI RCCGGY 1 cut(s) 320
BsrI ACTGG 4 cut(s) 1006, 1186, 1637, 1868
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 7 cut(s) 165, 931, 932, 995, 1336, 1413, 1507
BssT1I CCWWGG 2 cut(s) 165, 1507
Bst2UI CCWGG 6 cut(s) 869, 897, 933, 996, 1337, 1414
Bst4CI ACNGT 6 cut(s) 1080, 1168, 1397, 1462, 1481, 1911
BstAPI GCANNNNNTGC 1 cut(s) 725
BstBAI YACGTR 1 cut(s) 462
BstC8I GCNNGC 5 cut(s) 239, 402, 859, 1518, 1582
BstF5I GGATG 3 cut(s) 607, 1057, 1779
BstMAI GTCTC 2 cut(s) 141, 1051
BstMWI GCNNNNNNNGC 6 cut(s) 288, 397, 595, 725, 998, 1902
BstNI CCWGG 6 cut(s) 869, 897, 933, 996, 1337, 1414
BstNSI RCATGY 2 cut(s) 734, 1109
BstSCI CCNGG 6 cut(s) 867, 895, 931, 994, 1335, 1412
BstSFI CTRYAG 1 cut(s) 606
BstV1I GCAGC 5 cut(s) 13, 976, 1370, 1596, 1706
BstV2I GAAGAC 2 cut(s) 749, 762
BstX2I RGATCY 3 cut(s) 161, 208, 1539
BstYI RGATCY 3 cut(s) 161, 208, 1539
BsuRI GGCC 4 cut(s) 44, 291, 683, 1516
BtsCI GGATG 3 cut(s) 607, 1057, 1779
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 5 cut(s) 21, 663, 1026, 1164, 1313
BveI ACCTGC 1 cut(s) 1441
Cac8I GCNNGC 5 cut(s) 239, 402, 859, 1518, 1582
Cfr10I RCCGGY 1 cut(s) 320
Cfr13I GGNCC 2 cut(s) 541, 1514
CseI GACGC 1 cut(s) 1911
Csp6I GTAC 3 cut(s) 194, 499, 1327
CspAI ACCGGT 1 cut(s) 320
CviAII CATG 8 cut(s) 46, 220, 278, 628, 731, 804, 1106, 1714
CviQI GTAC 3 cut(s) 194, 499, 1327
DrdI GACNNNNNNGTC 1 cut(s) 1010
DseDI GACNNNNNNGTC 1 cut(s) 1010
EaeI YGGCCR 2 cut(s) 42, 289
Eco130I CCWWGG 2 cut(s) 165, 1507
Eco32I GATATC 1 cut(s) 571
Eco47I GGWCC 1 cut(s) 541
Eco57I CTGAAG 2 cut(s) 20, 465
Eco88I CYCGRG 1 cut(s) 70
EcoO109I RGGNCCY 1 cut(s) 541
EcoRII CCWGG 6 cut(s) 867, 895, 931, 994, 1335, 1412
EcoRV GATATC 1 cut(s) 571
EcoT14I CCWWGG 2 cut(s) 165, 1507
EcoT22I ATGCAT 1 cut(s) 1311
ErhI CCWWGG 2 cut(s) 165, 1507
FaeI CATG 8 cut(s) 49, 223, 281, 631, 734, 807, 1109, 1717
FalI AAGNNNNNCTT 2 cut(s) 782, 814
FaqI GGGAC 2 cut(s) 1649, 1897
FatI CATG 8 cut(s) 45, 219, 277, 627, 730, 803, 1105, 1713
FauI CCCGC 1 cut(s) 1645
FbaI TGATCA 2 cut(s) 3, 346
Fnu4HI GCNGC 5 cut(s) 27, 990, 1359, 1585, 1720
FokI GGATG 3 cut(s) 614, 1044, 1766
Fsp4HI GCNGC 5 cut(s) 27, 990, 1359, 1585, 1720
FspBI CTAG 3 cut(s) 815, 1118, 1899
GluI GCNGC 5 cut(s) 27, 990, 1359, 1585, 1720
GsuI CTGGAG 3 cut(s) 189, 1319, 1756
HaeIII GGCC 4 cut(s) 44, 291, 683, 1516
HapII CCGG 1 cut(s) 321
HgaI GACGC 1 cut(s) 1911
Hin1II CATG 8 cut(s) 49, 223, 281, 631, 734, 807, 1109, 1717
HincII GTYRAC 1 cut(s) 1231
HindII GTYRAC 1 cut(s) 1231
HindIII AAGCTT 2 cut(s) 32, 1446
HinfI GANTC 4 cut(s) 435, 758, 1044, 1888
HpaI GTTAAC 1 cut(s) 1231
HpaII CCGG 1 cut(s) 321
HphI GGTGA 3 cut(s) 1583, 1828, 1864
Hpy166II GTNNAC 4 cut(s) 64, 326, 1231, 1487
Hpy188I TCNGA 9 cut(s) 39, 81, 190, 417, 690, 763, 1220, 1344, 1349
Hpy188III TCNNGA 9 cut(s) 206, 344, 362, 443, 470, 584, 1735, 1783, 1914
Hpy8I GTNNAC 4 cut(s) 64, 326, 1231, 1487
Hpy99I CGWCG 1 cut(s) 552
HpyAV CCTTC 5 cut(s) 76, 130, 554, 769, 1272
HpyCH4III ACNGT 6 cut(s) 1080, 1168, 1397, 1462, 1481, 1911
HpyCH4IV ACGT 1 cut(s) 461
HpyF10VI GCNNNNNNNGC 6 cut(s) 288, 397, 595, 725, 998, 1902
HpySE526I ACGT 1 cut(s) 461
Hsp92II CATG 8 cut(s) 49, 223, 281, 631, 734, 807, 1109, 1717
Ksp22I TGATCA 2 cut(s) 3, 346
KspAI GTTAAC 1 cut(s) 1231
LmnI GCTCC 5 cut(s) 208, 381, 1338, 1418, 1934
Lsp1109I GCAGC 5 cut(s) 13, 976, 1370, 1596, 1706
LweI GCATC 4 cut(s) 592, 966, 1296, 1567
MaeI CTAG 3 cut(s) 815, 1118, 1899
MaeII ACGT 1 cut(s) 461
MaeIII GTNAC 4 cut(s) 52, 427, 1838, 1870
MboII GAAGA 9 cut(s) 377, 380, 754, 767, 1082, 1135, 1141, 1699, 1831
MfeI CAATTG 1 cut(s) 723
MflI RGATCY 3 cut(s) 161, 208, 1539
MlsI TGGCCA 2 cut(s) 44, 291
MluNI TGGCCA 2 cut(s) 44, 291
MlyI GAGTC 4 cut(s) 429, 752, 1053, 1882
MmeI TCCRAC 3 cut(s) 456, 1225, 1453
Mox20I TGGCCA 2 cut(s) 44, 291
Mph1103I ATGCAT 1 cut(s) 1311
MscI TGGCCA 2 cut(s) 44, 291
MseI TTAA 9 cut(s) 117, 216, 354, 387, 981, 1170, 1230, 1299, 1794
MslI CAYNNNNRTG 2 cut(s) 50, 1784
Msp20I TGGCCA 2 cut(s) 44, 291
MspA1I CMGCKG 1 cut(s) 1520
MspI CCGG 1 cut(s) 321
MspR9I CCNGG 6 cut(s) 869, 897, 933, 996, 1337, 1414
MunI CAATTG 1 cut(s) 723
Mva1269I GAATGC 3 cut(s) 841, 1309, 1679
MvaI CCWGG 6 cut(s) 869, 897, 933, 996, 1337, 1414
MwoI GCNNNNNNNGC 6 cut(s) 288, 397, 595, 725, 998, 1902
NlaIII CATG 8 cut(s) 49, 223, 281, 631, 734, 807, 1109, 1717
NlaIV GGNNCC 6 cut(s) 480, 493, 519, 542, 1376, 1936
NmuCI GTSAC 3 cut(s) 52, 1838, 1870
NsiI ATGCAT 1 cut(s) 1311
NspI RCATGY 2 cut(s) 734, 1109
PasI CCCWGGG 1 cut(s) 932
PciI ACATGT 1 cut(s) 1105
PcsI WCGNNNNNNNCGW 1 cut(s) 77
PctI GAATGC 3 cut(s) 841, 1309, 1679
PfoI TCCNGGA 1 cut(s) 867
PinAI ACCGGT 1 cut(s) 320
PkrI GCNGC 5 cut(s) 28, 991, 1360, 1586, 1721
PleI GAGTC 4 cut(s) 429, 752, 1052, 1882
PpsI GAGTC 4 cut(s) 429, 752, 1052, 1882
Ppu21I YACGTR 1 cut(s) 462
PpuMI RGGWCCY 1 cut(s) 541
PscI ACATGT 1 cut(s) 1105
Psp5II RGGWCCY 1 cut(s) 541
Psp6I CCWGG 6 cut(s) 867, 895, 931, 994, 1335, 1412
PspGI CCWGG 6 cut(s) 867, 895, 931, 994, 1335, 1412
PspN4I GGNNCC 6 cut(s) 480, 493, 519, 542, 1376, 1936
PspPI GGNCC 2 cut(s) 541, 1514
PspPPI RGGWCCY 1 cut(s) 541
PsuI RGATCY 3 cut(s) 161, 208, 1539
PvuII CAGCTG 1 cut(s) 1520
RsaI GTAC 3 cut(s) 195, 500, 1328
RsaNI GTAC 3 cut(s) 194, 499, 1327
RseI CAYNNNNRTG 2 cut(s) 50, 1784
SaqAI TTAA 9 cut(s) 117, 216, 354, 387, 981, 1170, 1230, 1299, 1794
SatI GCNGC 5 cut(s) 27, 990, 1359, 1585, 1720
Sau96I GGNCC 2 cut(s) 541, 1514
ScaI AGTACT 2 cut(s) 195, 500
SchI GAGTC 4 cut(s) 429, 752, 1053, 1882
ScrFI CCNGG 6 cut(s) 869, 897, 933, 996, 1337, 1414
SfaNI GCATC 4 cut(s) 592, 966, 1296, 1567
SfcI CTRYAG 1 cut(s) 606
SgrAI CRCCGGYG 1 cut(s) 320
SinI GGWCC 1 cut(s) 541
SmiMI CAYNNNNRTG 2 cut(s) 50, 1784
SmlI CTYRAG 1 cut(s) 584
SmoI CTYRAG 1 cut(s) 584
SsiI CCGC 4 cut(s) 918, 1378, 1406, 1652
SspI AATATT 1 cut(s) 267
SspMI CTAG 3 cut(s) 815, 1118, 1899
StyD4I CCNGG 6 cut(s) 867, 895, 931, 994, 1335, 1412
StyI CCWWGG 2 cut(s) 165, 1507
TaaI ACNGT 6 cut(s) 1080, 1168, 1397, 1462, 1481, 1911
TaiI ACGT 1 cut(s) 464
TaqI TCGA 2 cut(s) 621, 969
TatI WGTACW 2 cut(s) 193, 498
Tru1I TTAA 9 cut(s) 117, 216, 354, 387, 981, 1170, 1230, 1299, 1794
Tru9I TTAA 9 cut(s) 117, 216, 354, 387, 981, 1170, 1230, 1299, 1794
TscAI CASTG 5 cut(s) 28, 670, 1033, 1171, 1320
TseFI GTSAC 3 cut(s) 52, 1838, 1870
TseI GCWGC 5 cut(s) 26, 989, 1358, 1584, 1719
Tsp45I GTSAC 3 cut(s) 52, 1838, 1870
TspDTI ATGAA 8 cut(s) 768, 848, 921, 1046, 1116, 1458, 1490, 1686
TspRI CASTG 5 cut(s) 28, 670, 1033, 1171, 1320
VpaK11BI GGWCC 1 cut(s) 541
XapI RAATTY 4 cut(s) 100, 673, 977, 1810
XceI RCATGY 2 cut(s) 734, 1109
XspI CTAG 3 cut(s) 815, 1118, 1899
ZrmI AGTACT 2 cut(s) 195, 500
Zsp2I ATGCAT 1 cut(s) 1311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.