Rmu_sc0005442.1_g000015

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005442.1
OG0013694
Physical Location & Seq
Reverse (-)
63897 .. 65384
1488 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1488 bp
atgtatgcgtctttcgggcttgtggaggaggcaaggaggatattgctagacaactcggcgtcgtctgatgttgtttgttggaatgcaatgattgatgggtacatgaaatgtggggatgcagaagcagctaaggagttgtttgataagcacatgccaattaagaatgttgggtcttggaatgcaatggtgagtggttttgctaagtgtgggaggttgacagatgcaagggcggtgtttgatgaaatgattgagagagatgagatttcttggagtgctatgattgatggttatgtgcaaggagggtatcacaaggaggcattggaagttttcaatgagatgcaaaaggagaagattgttagtccaaggaaattcgtattgtcgagtgtgttagctgcttgtgccaatgtgggagcacttgatcaaggaaagtggattcatggttatattaagaagaactcaattcaggtggatgcactactggggactgctttggttgatatgtatgccaaatgtgggaggcttgacatggcatgggaagtgtttgaaaatgtgaaacagaaagaggtttcgacatggaatgccatgattggtgggcttgctatgcacggccgagcagatgatgcattggatgttttcttcaagatgcaagggaataatttggaaccaaatgggattacttttctgaatgctctaagtgcttgtgctcatgggggttttgttgatgaaggtcttacaattttcagttctatgaaggaaatgtatggtatggaggctgaggtagagcactatggttgtgtggttgatatgtttggaagggcaggacttttggaagaggcagagcaggttataaattcaatgccaattgaacctagtgcagctgtttatggtgccctgttaggcgcttgcaggatacatggaaatgttgaaatgggtgagagagtagggaggattttgcttgaattggaaccgcaaaacagtggacgttacgcattgttatctaacatctatgcaaaggcaggtatgtgggacgatgttgcagaggtaagaaaattgatgaaggaaaggggagtaaagactattcctggaattagcatggttgatattggtggcaaagtacatgaattcaaaatgggagatgggtcacacccacaaatgaaggaggtctacttgatgcttgaaaagattatagaaaagctggagacagagggttattcaccgaatacatctcaagttttgtttgacattgcagaggaggagaaggaaactgcactgcggtaccacagtgagaagcttgcaattgctttcggagtacttaacacaaaacctggaacaaccatccgggttgtgaagaacttgaggatttgtgaggactgtcattctgccataaagctcttttcacaatcatacaaccgggatataattgtgagggaccgtatgcgctatcatcattttagaaatggaaggtgttcgtgcaaggatttttggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

495

Amino Acids

55.3

Weight (kDa)

5.58

Isoelectric Point (pI)

33.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 23 - 48 3.6e-06 PPR repeat family
PPR PF01535 25 - 49 4.7e-06 PPR repeat
PPR PF01535 58 - 85 3.1e-07 PPR repeat
PPR_2 PF13041 86 - 118 7.7e-07 PPR repeat family
PPR PF01535 88 - 117 9.4e-09 PPR repeat
PPR_2 PF13041 189 - 235 9.4e-09 PPR repeat family
PPR PF01535 190 - 219 3.6e-07 PPR repeat
E_motif PF20431 305 - 366 3.2e-22 E motif
DYW_deaminase PF14432 403 - 495 1.3e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 848
Acc36I ACCTGC 2 cut(s) 832, 1007
Acc65I GGTACC 1 cut(s) 1275
AccB1I GGYRCC 2 cut(s) 887, 1275
AccI GTMKAC 1 cut(s) 1164
AciI CCGC 3 cut(s) 230, 968, 1273
AcoI YGGCCR 1 cut(s) 607
AcsI RAATTY 3 cut(s) 368, 850, 1121
AcyI GRCGYC 1 cut(s) 59
AfaI GTAC 4 cut(s) 101, 1116, 1277, 1311
AfiI CCNNNNNNNGG 1 cut(s) 513
AgsI TTSAA 9 cut(s) 331, 545, 640, 855, 866, 926, 959, 1126, 1178
AjnI CCWGG 2 cut(s) 1081, 1324
AluBI AGCT 6 cut(s) 128, 392, 878, 1195, 1291, 1390
AluI AGCT 6 cut(s) 128, 392, 878, 1195, 1291, 1390
Alw21I GWGCWC 3 cut(s) 415, 706, 786
Alw26I GTCTC 1 cut(s) 1193
AoxI GGCC 1 cut(s) 607
ApeKI GCWGC 3 cut(s) 125, 392, 875
ApoI RAATTY 3 cut(s) 368, 850, 1121
Asp700I GAANNNNTTC 1 cut(s) 1465
Asp718I GGTACC 1 cut(s) 1275
AspLEI GCGC 2 cut(s) 902, 1440
AspS9I GGNCC 1 cut(s) 1429
AsuC2I CCSGG 2 cut(s) 1340, 1412
AsuHPI GGTGA 3 cut(s) 199, 944, 1206
AvaII GGWCC 1 cut(s) 1429
BaeGI GKGCMC 1 cut(s) 892
BaeI ACNNNNGTAYC 4 cut(s) 1259, 1267, 1292, 1300
BanI GGYRCC 2 cut(s) 887, 1275
BarI GAAGNNNNNNTAC 2 cut(s) 1148, 1180
Bbv12I GWGCWC 3 cut(s) 415, 706, 786
BbvCI CCTCAGC 1 cut(s) 774
BbvI GCAGC 3 cut(s) 137, 379, 887
BccI CCATC 4 cut(s) 89, 278, 1130, 1343
BceAI ACGGC 1 cut(s) 622
BciT130I CCWGG 2 cut(s) 1083, 1326
BciVI GTATCC 1 cut(s) 903
BclI TGATCA 1 cut(s) 418
BcnI CCSGG 2 cut(s) 1340, 1412
BcoDI GTCTC 1 cut(s) 1193
BfaI CTAG 2 cut(s) 47, 870
BfoI RGCGCY 1 cut(s) 903
BfuAI ACCTGC 2 cut(s) 832, 1007
BfuI GTATCC 1 cut(s) 903
BisI GCNGC 3 cut(s) 126, 393, 876
BlsI GCNGC 3 cut(s) 127, 394, 877
BmcAI AGTACT 1 cut(s) 1311
Bme1390I CCNGG 4 cut(s) 1083, 1326, 1340, 1412
Bme18I GGWCC 1 cut(s) 1429
BmgT120I GGNCC 1 cut(s) 1429
BmiI GGNNCC 5 cut(s) 663, 889, 966, 1277, 1430
BmrFI CCNGG 4 cut(s) 1083, 1326, 1340, 1412
BmrI ACTGGG 1 cut(s) 488
BmsI GCATC 7 cut(s) 106, 211, 327, 460, 610, 633, 1161
BmuI ACTGGG 1 cut(s) 488
BpmI CTGGAG 1 cut(s) 1217
Bpu10I CCTNAGC 2 cut(s) 129, 774
BpuEI CTTGAG 2 cut(s) 1212, 1375
BpuMI CCSGG 2 cut(s) 1340, 1412
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 1 cut(s) 362
Bsc4I CCNNNNNNNGG 1 cut(s) 513
Bse1I ACTGG 1 cut(s) 483
Bse3DI GCAATG 3 cut(s) 93, 189, 1242
BseBI CCWGG 2 cut(s) 1083, 1326
BseDI CCNNGG 1 cut(s) 362
BseGI GGATG 4 cut(s) 121, 475, 634, 1335
BseLI CCNNNNNNNGG 1 cut(s) 513
BseMI GCAATG 3 cut(s) 93, 189, 1242
BseMII CTCAG 1 cut(s) 765
BseNI ACTGG 1 cut(s) 483
BseRI GAGGAG 3 cut(s) 41, 1265, 1268
BseSI GKGCMC 1 cut(s) 892
BseX3I CGGCCG 1 cut(s) 607
BseXI GCAGC 3 cut(s) 137, 379, 887
BsgI GTGCAG 2 cut(s) 894, 1251
Bsh1285I CGRYCG 1 cut(s) 610
BshFI GGCC 1 cut(s) 609
BshNI GGYRCC 2 cut(s) 887, 1275
BsiEI CGRYCG 1 cut(s) 610
BsiHKAI GWGCWC 3 cut(s) 415, 706, 786
BsiSI CCGG 2 cut(s) 1339, 1411
BslFI GGGAC 3 cut(s) 496, 1040, 1442
BslI CCNNNNNNNGG 1 cut(s) 513
BsmAI GTCTC 1 cut(s) 1193
BsmFI GGGAC 3 cut(s) 496, 1040, 1442
BsmI GAATGC 4 cut(s) 88, 184, 583, 691
BsnI GGCC 1 cut(s) 609
Bsp1286I GDGCHC 4 cut(s) 415, 706, 786, 892
Bsp143I GATC 1 cut(s) 418
BspACI CCGC 3 cut(s) 230, 968, 1273
BspANI GGCC 1 cut(s) 609
BspCNI CTCAG 1 cut(s) 766
BspLI GGNNCC 5 cut(s) 663, 889, 966, 1277, 1430
BspMI ACCTGC 2 cut(s) 832, 1007
BspT107I GGYRCC 2 cut(s) 887, 1275
BsrDI GCAATG 3 cut(s) 93, 189, 1242
BsrI ACTGG 1 cut(s) 483
BssECI CCNNGG 1 cut(s) 362
BssMI GATC 1 cut(s) 418
BssNI GRCGYC 1 cut(s) 59
BssT1I CCWWGG 1 cut(s) 362
Bst2UI CCWGG 2 cut(s) 1083, 1326
Bst4CI ACNGT 4 cut(s) 977, 1283, 1373, 1433
Bst6I CTCTTC 1 cut(s) 825
BstACI GRCGYC 1 cut(s) 59
BstAPI GCANNNNNTGC 1 cut(s) 620
BstC8I GCNNGC 3 cut(s) 597, 904, 1293
BstDEI CTNAG 4 cut(s) 129, 201, 692, 774
BstF5I GGATG 4 cut(s) 121, 475, 634, 1335
BstH2I RGCGCY 1 cut(s) 903
BstHHI GCGC 2 cut(s) 902, 1440
BstKTI GATC 1 cut(s) 421
BstMAI GTCTC 1 cut(s) 1193
BstMBI GATC 1 cut(s) 418
BstMCI CGRYCG 1 cut(s) 610
BstMWI GCNNNNNNNGC 5 cut(s) 125, 398, 601, 620, 695
BstNI CCWGG 2 cut(s) 1083, 1326
BstNSI RCATGY 1 cut(s) 154
BstSCI CCNGG 4 cut(s) 1081, 1324, 1338, 1410
BstSLI GKGCMC 1 cut(s) 892
BstV1I GCAGC 3 cut(s) 137, 379, 887
BstZI CGGCCG 1 cut(s) 607
BsuI GTATCC 1 cut(s) 903
BsuRI GGCC 1 cut(s) 609
BtsCI GGATG 4 cut(s) 121, 475, 634, 1335
BtsI GCAGTG 1 cut(s) 1268
BtsIMutI CAGTG 3 cut(s) 982, 1268, 1288
BveI ACCTGC 2 cut(s) 832, 1007
Cac8I GCNNGC 3 cut(s) 597, 904, 1293
CfoI GCGC 2 cut(s) 902, 1440
Cfr13I GGNCC 1 cut(s) 1429
CseI GACGC 1 cut(s) 48
Csp6I GTAC 4 cut(s) 100, 1115, 1276, 1310
CspCI CAANNNNNGTGG 4 cut(s) 410, 445, 447, 482
CviQI GTAC 4 cut(s) 100, 1115, 1276, 1310
DdeI CTNAG 4 cut(s) 129, 201, 692, 774
DpnI GATC 1 cut(s) 420
DpnII GATC 1 cut(s) 418
EaeI YGGCCR 1 cut(s) 607
EagI CGGCCG 1 cut(s) 607
Eam1104I CTCTTC 1 cut(s) 825
EarI CTCTTC 1 cut(s) 825
EclXI CGGCCG 1 cut(s) 607
Eco130I CCWWGG 1 cut(s) 362
Eco47I GGWCC 1 cut(s) 1429
Eco52I CGGCCG 1 cut(s) 607
EcoRI GAATTC 1 cut(s) 1121
EcoRII CCWGG 2 cut(s) 1081, 1324
EcoT14I CCWWGG 1 cut(s) 362
EcoT22I ATGCAT 1 cut(s) 625
ErhI CCWWGG 1 cut(s) 362
FaqI GGGAC 3 cut(s) 496, 1040, 1442
FbaI TGATCA 1 cut(s) 418
FblI GTMKAC 1 cut(s) 1164
Fnu4HI GCNGC 3 cut(s) 126, 393, 876
FokI GGATG 4 cut(s) 128, 482, 641, 1322
Fsp4HI GCNGC 3 cut(s) 126, 393, 876
FspBI CTAG 2 cut(s) 47, 870
GlaI GCGC 2 cut(s) 901, 1439
GluI GCNGC 3 cut(s) 126, 393, 876
GsuI CTGGAG 1 cut(s) 1217
HaeII RGCGCY 1 cut(s) 903
HaeIII GGCC 1 cut(s) 609
HapII CCGG 2 cut(s) 1339, 1411
HgaI GACGC 1 cut(s) 48
HhaI GCGC 2 cut(s) 902, 1440
Hin1I GRCGYC 1 cut(s) 59
Hin6I GCGC 2 cut(s) 900, 1438
HinP1I GCGC 2 cut(s) 900, 1438
HincII GTYRAC 1 cut(s) 216
HindII GTYRAC 1 cut(s) 216
HindIII AAGCTT 1 cut(s) 1289
HinfI GANTC 1 cut(s) 433
HpaII CCGG 2 cut(s) 1339, 1411
HphI GGTGA 3 cut(s) 199, 944, 1206
Hpy166II GTNNAC 3 cut(s) 216, 980, 1165
Hpy188I TCNGA 3 cut(s) 67, 684, 1307
Hpy188III TCNNGA 1 cut(s) 640
Hpy8I GTNNAC 3 cut(s) 216, 980, 1165
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 7 cut(s) 719, 745, 807, 1051, 1150, 1252, 1455
HpyCH4III ACNGT 4 cut(s) 977, 1283, 1373, 1433
HpyCH4IV ACGT 1 cut(s) 982
HpyF10VI GCNNNNNNNGC 5 cut(s) 125, 398, 601, 620, 695
HpyF3I CTNAG 4 cut(s) 129, 201, 692, 774
HpySE526I ACGT 1 cut(s) 982
Hsp92I GRCGYC 1 cut(s) 59
HspAI GCGC 2 cut(s) 900, 1438
KpnI GGTACC 1 cut(s) 1279
Ksp22I TGATCA 1 cut(s) 418
Kzo9I GATC 1 cut(s) 418
LmnI GCTCC 1 cut(s) 410
Lsp1109I GCAGC 3 cut(s) 137, 379, 887
LweI GCATC 7 cut(s) 106, 211, 327, 460, 610, 633, 1161
MaeI CTAG 2 cut(s) 47, 870
MaeII ACGT 1 cut(s) 982
MaeIII GTNAC 2 cut(s) 983, 1140
MalI GATC 1 cut(s) 420
MboI GATC 1 cut(s) 418
MboII GAAGA 5 cut(s) 361, 463, 628, 842, 1360
MfeI CAATTG 2 cut(s) 861, 1296
MhlI GDGCHC 4 cut(s) 415, 706, 786, 892
MmeI TCCRAC 1 cut(s) 59
Mph1103I ATGCAT 1 cut(s) 625
MroXI GAANNNNTTC 1 cut(s) 1465
MseI TTAA 3 cut(s) 159, 447, 1314
MslI CAYNNNNRTG 1 cut(s) 918
MspA1I CMGCKG 1 cut(s) 878
MspI CCGG 2 cut(s) 1339, 1411
MspR9I CCNGG 4 cut(s) 1083, 1326, 1340, 1412
MunI CAATTG 2 cut(s) 861, 1296
Mva1269I GAATGC 4 cut(s) 88, 184, 583, 691
MvaI CCWGG 2 cut(s) 1083, 1326
MwoI GCNNNNNNNGC 5 cut(s) 125, 398, 601, 620, 695
NciI CCSGG 2 cut(s) 1340, 1412
NdeII GATC 1 cut(s) 418
NlaIV GGNNCC 5 cut(s) 663, 889, 966, 1277, 1430
NmeAIII GCCGAG 2 cut(s) 35, 635
NmuCI GTSAC 1 cut(s) 1140
NsiI ATGCAT 1 cut(s) 625
NspI RCATGY 1 cut(s) 154
PctI GAATGC 4 cut(s) 88, 184, 583, 691
PdmI GAANNNNTTC 1 cut(s) 1465
PfeI GAWTC 1 cut(s) 433
PfoI TCCNGGA 1 cut(s) 1081
PkrI GCNGC 3 cut(s) 127, 394, 877
PsiI TTATAA 1 cut(s) 848
Psp6I CCWGG 2 cut(s) 1081, 1324
PspGI CCWGG 2 cut(s) 1081, 1324
PspN4I GGNNCC 5 cut(s) 663, 889, 966, 1277, 1430
PspPI GGNCC 1 cut(s) 1429
PvuII CAGCTG 1 cut(s) 878
RsaI GTAC 4 cut(s) 101, 1116, 1277, 1311
RsaNI GTAC 4 cut(s) 100, 1115, 1276, 1310
RseI CAYNNNNRTG 1 cut(s) 918
SaqAI TTAA 3 cut(s) 159, 447, 1314
SatI GCNGC 3 cut(s) 126, 393, 876
Sau3AI GATC 1 cut(s) 418
Sau96I GGNCC 1 cut(s) 1429
ScaI AGTACT 1 cut(s) 1311
ScrFI CCNGG 4 cut(s) 1083, 1326, 1340, 1412
SduI GDGCHC 4 cut(s) 415, 706, 786, 892
SfaNI GCATC 7 cut(s) 106, 211, 327, 460, 610, 633, 1161
SinI GGWCC 1 cut(s) 1429
SmiMI CAYNNNNRTG 1 cut(s) 918
SmlI CTYRAG 2 cut(s) 1227, 1354
SmoI CTYRAG 2 cut(s) 1227, 1354
SsiI CCGC 3 cut(s) 230, 968, 1273
SspMI CTAG 2 cut(s) 47, 870
StyD4I CCNGG 4 cut(s) 1081, 1324, 1338, 1410
StyI CCWWGG 1 cut(s) 362
TaaI ACNGT 4 cut(s) 977, 1283, 1373, 1433
TaiI ACGT 1 cut(s) 985
TaqI TCGA 2 cut(s) 380, 569
TatI WGTACW 2 cut(s) 1114, 1309
TfiI GAWTC 1 cut(s) 433
Tru1I TTAA 3 cut(s) 159, 447, 1314
Tru9I TTAA 3 cut(s) 159, 447, 1314
TscAI CASTG 3 cut(s) 982, 1275, 1288
TseFI GTSAC 1 cut(s) 1140
TseI GCWGC 3 cut(s) 125, 392, 875
Tsp45I GTSAC 1 cut(s) 1140
TspDTI ATGAA 8 cut(s) 119, 255, 425, 738, 764, 1070, 1134, 1169
TspRI CASTG 3 cut(s) 982, 1275, 1288
VpaK11BI GGWCC 1 cut(s) 1429
XapI RAATTY 3 cut(s) 368, 850, 1121
XceI RCATGY 1 cut(s) 154
XmiI GTMKAC 1 cut(s) 1164
XmnI GAANNNNTTC 1 cut(s) 1465
XspI CTAG 2 cut(s) 47, 870
ZrmI AGTACT 1 cut(s) 1311
Zsp2I ATGCAT 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.