Rmu_sc0005891.1_g000011

Translation initiation factor eIF-2B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005891.1
OG0005877
Physical Location & Seq
Reverse (-)
34667 .. 43718
9052 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2175 bp
atgggtgcacagaagaaaggcgcgtctagggcttcagaagaccctgaggaattggcacgtgtcccactccaggccatcctcctagccgatagtttcaccaccaaattccgacccatcactcttgaacgccccaaggtgctgttgccgttggtcaatgccccgatgatcagttacacgttggcatggcttgagtctgctggcgttgaggaggtttttgttttctgctgtgctcactccaagcaagtcatcacttatttggacaattctgagttttcccaaccaaacttcaccgtcaccacaattgagtcacatgattctgttagtgctggagatgctctgcgcttgatatatgagcgcaatgtgatacatggagattttgtcctagtcagtggggataccgtgagcaacatgtcgcttactcaggtgcttcaagaacataaagagagaaggaagaaggatagtaatgctgtaatgacaattgttattaaacggtcaaagccttcaccaatcactcgtcaatctcgactgggcactgatgagctctttatggcagtagatcccaacacaaagcagctcttatattatgaggacaaggcagaccatgcaaagggatctatatatcttgataagttattgcttgctgataagtcttcaatttctctgcataatgacaaacaggattgctatattgacatctgctctcctgaagtcctcagccttttcacagacaattttgactatcaacatttacgtcgtcattttttaaagggattgcttcttgatgatattatgggttacaaaatattcacacatgaaattcactcgacttatgcggctaggattgacaacttcagaagctatgatacaatcagtaaggacataattcagagatggacttacccattggtgccagatgttaagtttttcggagattcttcaacgaagctggaaagacgggggatgtatcgagcctcagatatagggcaatcacgatctgcacagattggtccttttacagtcattgggaatggcaccaagattggggacaacacaaaaatttcaaattctgttattggagaagggtgttctattggaccaaacgtttccatagaaggttcttatatatgggataatgtcaccattgaagatggctgcaagctaaggcatgctatagtatgtgatggagtgataatgaagtctggggcagttttggaatcgggtgtagttttgtctttcaaggttgtgataggacaacaatttattgtcccttcatactcgaaggtatctttacttcaacaaccaataaagcaagacagtgatgaggagctagagtatgctgacaatagcactgggattgcagaaatttcccctcctatacatgcaccggatatatcgaacggggaaatagcgaaccaacaacaggagactcggtgttggcctacatctgagcttggtattggtggggctggttatgtttggtcagtttgtgaaggaggccatgatgaagagtggaggcattcagttgctcccattcctgcagagaaacttgctgaggtgatacaagctgcagaggatgatctggagttagatccagatggcagcagtctcccaccttcaggagagctgaaacttgattctgattctgatgaagagtatagagatggttcctactttgaaaatgaggttgaagcaaccttcctaagagctgtggatgaaaatattaaagtagagaatgtcgtcttagaagtgaactcactgagattgtcattcaacaagcaaactgtacactgtgctggagctatattttattcaatgatgaaattggcattagaaactccacataattcagcagttgaattggtccaagctactacgaaggtaattacaaaatggaaaggactgctgaagtattacctatcagacattgatgaagagatagaagtaataatgaaatttgaagatatgtgtttggagtccgctaaggagttctcccccgtgttcgataagatactctatcaactgtatgaccaagaagttatacaggaagaggctattctgaggtgggatgatgaaaagagacatgctgatgaatcagatagaatttttgttaggcaggctgaaacatttatccaatggttgagagaggcatctgaagaagaggatgaggaagagtag

Protein Analysis

724

Amino Acids

81.43

Weight (kDa)

4.74

Isoelectric Point (pI)

48.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTP_transferase PF00483 25 - 164 7.1e-12 Nucleotidyl transferase
LbH_EIF2B PF25084 318 - 421 2.6e-39 EIF2B subunit epsilon LbH domain
GMPPB_C PF25087 332 - 415 2.8e-10 GMPPB C-terminal domain
Hexapep PF00132 339 - 372 1.4e-07 Bacterial transferase hexapeptide (six repeats)
W2 PF02020 645 - 723 7.9e-18 eIF4-gamma/eIF5/eIF2-epsilon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 907, 1031
AccII CGCG 1 cut(s) 23
AciI CCGC 2 cut(s) 833, 1977
AclI AACGTT 1 cut(s) 1101
AclWI GGATC 3 cut(s) 551, 619, 1583
AcsI RAATTY 7 cut(s) 104, 816, 1056, 1063, 1362, 1952, 2100
AcuI CTGAAG 6 cut(s) 18, 726, 835, 1599, 1925, 2172
AcvI CACGTG 1 cut(s) 59
AfaI GTAC 1 cut(s) 1785
AfiI CCNNNNNNNGG 5 cut(s) 70, 607, 1041, 1420, 1616
AflIII ACRYGT 3 cut(s) 58, 174, 408
AjnI CCWGG 1 cut(s) 69
Alw21I GWGCWC 3 cut(s) 10, 232, 543
Alw26I GTCTC 3 cut(s) 1418, 1610, 2071
Alw44I GTGCAC 1 cut(s) 6
AlwI GGATC 3 cut(s) 551, 619, 1583
AoxI GGCC 3 cut(s) 72, 1436, 1495
ApaLI GTGCAC 1 cut(s) 6
ApeKI GCWGC 4 cut(s) 571, 1152, 1565, 1599
ApoI RAATTY 7 cut(s) 104, 816, 1056, 1063, 1362, 1952, 2100
AspLEI GCGC 3 cut(s) 23, 342, 357
AspS9I GGNCC 3 cut(s) 1007, 1094, 1861
AsuHPI GGTGA 6 cut(s) 88, 280, 286, 495, 1129, 1567
AvaII GGWCC 3 cut(s) 1007, 1094, 1861
AxyI CCTNAGG 1 cut(s) 45
BaeGI GKGCMC 2 cut(s) 10, 533
BanI GGYRCC 2 cut(s) 907, 1031
BanII GRGCYC 1 cut(s) 543
BarI GAAGNNNNNNTAC 4 cut(s) 847, 879, 1275, 1307
BbrPI CACGTG 1 cut(s) 59
BbsI GAAGAC 2 cut(s) 45, 642
Bbv12I GWGCWC 3 cut(s) 10, 232, 543
BbvCI CCTCAGC 2 cut(s) 713, 1551
BbvI GCAGC 4 cut(s) 583, 1139, 1552, 1611
BccI CCATC 7 cut(s) 83, 122, 885, 1142, 1175, 1589, 1655
BceAI ACGGC 1 cut(s) 130
BciT130I CCWGG 1 cut(s) 71
BciVI GTATCC 1 cut(s) 388
BclI TGATCA 1 cut(s) 165
BcoDI GTCTC 3 cut(s) 1418, 1610, 2071
BfaI CTAG 5 cut(s) 27, 83, 383, 837, 1328
BfmI CTRYAG 3 cut(s) 1170, 1536, 1566
BfuI GTATCC 1 cut(s) 388
BisI GCNGC 5 cut(s) 572, 834, 1153, 1566, 1600
BlsI GCNGC 5 cut(s) 573, 835, 1154, 1567, 1601
Bme1390I CCNGG 1 cut(s) 71
Bme18I GGWCC 3 cut(s) 1007, 1094, 1861
BmgT120I GGNCC 3 cut(s) 1007, 1094, 1861
BmiI GGNNCC 3 cut(s) 909, 1033, 1666
BmrFI CCNGG 1 cut(s) 71
BmrI ACTGGG 2 cut(s) 536, 1359
BmsI GCATC 2 cut(s) 322, 2156
BmuI ACTGGG 2 cut(s) 536, 1359
BpiI GAAGAC 2 cut(s) 45, 642
BpmI CTGGAG 4 cut(s) 53, 348, 1601, 1815
Bpu10I CCTNAGC 4 cut(s) 713, 1160, 1551, 1980
BpuEI CTTGAG 1 cut(s) 209
BsaAI YACGTR 1 cut(s) 59
BsaJI CCNNGG 1 cut(s) 132
BsaWI WCCGGW 1 cut(s) 1384
BsaXI ACNNNNNCTCC 6 cut(s) 363, 393, 1316, 1346, 1415, 1445
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 607, 1041, 1420, 1616
Bse1I ACTGG 2 cut(s) 531, 1354
Bse21I CCTNAGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 364
BseBI CCWGG 1 cut(s) 71
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 6 cut(s) 75, 966, 1579, 1717, 2071, 2167
BseLI CCNNNNNNNGG 5 cut(s) 70, 607, 1041, 1420, 1616
BseMI GCAATG 1 cut(s) 364
BseMII CTCAG 9 cut(s) 36, 258, 434, 727, 987, 1437, 1542, 1748, 2048
BseNI ACTGG 2 cut(s) 531, 1354
BseRI GAGGAG 2 cut(s) 221, 1337
BseSI GKGCMC 2 cut(s) 10, 533
BseXI GCAGC 4 cut(s) 583, 1139, 1552, 1611
BsgI GTGCAG 1 cut(s) 981
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 3 cut(s) 74, 1438, 1497
BshNI GGYRCC 2 cut(s) 907, 1031
BsiHKAI GWGCWC 3 cut(s) 10, 232, 543
BsiSI CCGG 1 cut(s) 1385
BslFI GGGAC 3 cut(s) 47, 1058, 1250
BslI CCNNNNNNNGG 5 cut(s) 70, 607, 1041, 1420, 1616
BsmAI GTCTC 3 cut(s) 1418, 1610, 2071
BsmFI GGGAC 3 cut(s) 47, 1058, 1250
BsmI GAATGC 1 cut(s) 1516
BsnI GGCC 3 cut(s) 74, 1438, 1497
Bsp1286I GDGCHC 4 cut(s) 10, 232, 533, 543
Bsp1407I TGTACA 1 cut(s) 1783
Bsp143I GATC 6 cut(s) 165, 556, 611, 992, 1576, 1588
BspACI CCGC 2 cut(s) 833, 1977
BspANI GGCC 3 cut(s) 74, 1438, 1497
BspCNI CTCAG 9 cut(s) 37, 259, 433, 726, 986, 1438, 1543, 1749, 2049
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 3 cut(s) 909, 1033, 1666
BspMAI CTGCAG 2 cut(s) 1540, 1570
BspPI GGATC 3 cut(s) 551, 619, 1583
BspT107I GGYRCC 2 cut(s) 907, 1031
BsrDI GCAATG 1 cut(s) 364
BsrGI TGTACA 1 cut(s) 1783
BsrI ACTGG 2 cut(s) 531, 1354
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 6 cut(s) 165, 556, 611, 992, 1576, 1588
BssT1I CCWWGG 1 cut(s) 132
Bst2UI CCWGG 1 cut(s) 71
Bst4CI ACNGT 8 cut(s) 292, 400, 492, 1018, 1316, 1783, 1790, 2022
Bst6I CTCTTC 6 cut(s) 1500, 1644, 1926, 2040, 2151, 2163
BstAPI GCANNNNNTGC 1 cut(s) 602
BstAUI TGTACA 1 cut(s) 1783
BstBAI YACGTR 1 cut(s) 59
BstC8I GCNNGC 5 cut(s) 199, 639, 1157, 1167, 2115
BstF5I GGATG 6 cut(s) 75, 966, 1579, 1717, 2071, 2167
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 3 cut(s) 23, 342, 357
BstKTI GATC 6 cut(s) 168, 559, 614, 995, 1579, 1591
BstMAI GTCTC 3 cut(s) 1418, 1610, 2071
BstMBI GATC 6 cut(s) 165, 556, 611, 992, 1576, 1588
BstMWI GCNNNNNNNGC 3 cut(s) 29, 332, 602
BstNI CCWGG 1 cut(s) 71
BstNSI RCATGY 4 cut(s) 412, 1169, 1382, 2084
BstSCI CCNGG 1 cut(s) 69
BstSFI CTRYAG 3 cut(s) 1170, 1536, 1566
BstSLI GKGCMC 2 cut(s) 10, 533
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 4 cut(s) 583, 1139, 1552, 1611
BstV2I GAAGAC 2 cut(s) 45, 642
BstX2I RGATCY 3 cut(s) 556, 611, 1588
BstYI RGATCY 3 cut(s) 556, 611, 1588
Bsu36I CCTNAGG 1 cut(s) 45
BsuI GTATCC 1 cut(s) 388
BsuRI GGCC 3 cut(s) 74, 1438, 1497
BtsCI GGATG 6 cut(s) 75, 966, 1579, 1717, 2071, 2167
BtsIMutI CAGTG 6 cut(s) 394, 531, 1321, 1347, 1754, 1786
Cac8I GCNNGC 5 cut(s) 199, 639, 1157, 1167, 2115
CfoI GCGC 3 cut(s) 23, 342, 357
Cfr13I GGNCC 3 cut(s) 1007, 1094, 1861
CseI GACGC 1 cut(s) 12
Csp6I GTAC 1 cut(s) 1784
CviQI GTAC 1 cut(s) 1784
DpnI GATC 6 cut(s) 167, 558, 613, 994, 1578, 1590
DpnII GATC 6 cut(s) 165, 556, 611, 992, 1576, 1588
DraI TTTAAA 1 cut(s) 765
Eam1104I CTCTTC 6 cut(s) 1500, 1644, 1926, 2040, 2151, 2163
EarI CTCTTC 6 cut(s) 1500, 1644, 1926, 2040, 2151, 2163
Ecl136II GAGCTC 1 cut(s) 541
Eco130I CCWWGG 1 cut(s) 132
Eco24I GRGCYC 1 cut(s) 543
Eco47I GGWCC 3 cut(s) 1007, 1094, 1861
Eco53kI GAGCTC 1 cut(s) 541
Eco57I CTGAAG 6 cut(s) 18, 726, 835, 1599, 1925, 2172
Eco72I CACGTG 1 cut(s) 59
Eco81I CCTNAGG 1 cut(s) 45
EcoICRI GAGCTC 1 cut(s) 541
EcoRII CCWGG 1 cut(s) 69
EcoT14I CCWWGG 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 543
ErhI CCWWGG 1 cut(s) 132
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 3 cut(s) 47, 1058, 1250
FbaI TGATCA 1 cut(s) 165
Fnu4HI GCNGC 5 cut(s) 572, 834, 1153, 1566, 1600
FokI GGATG 5 cut(s) 62, 973, 1586, 1724, 2078
FriOI GRGCYC 1 cut(s) 543
Fsp4HI GCNGC 5 cut(s) 572, 834, 1153, 1566, 1600
FspBI CTAG 5 cut(s) 27, 83, 383, 837, 1328
GlaI GCGC 3 cut(s) 22, 341, 356
GluI GCNGC 5 cut(s) 572, 834, 1153, 1566, 1600
GsuI CTGGAG 4 cut(s) 53, 348, 1601, 1815
HaeIII GGCC 3 cut(s) 74, 1438, 1497
HapII CCGG 1 cut(s) 1385
HgaI GACGC 1 cut(s) 12
HhaI GCGC 3 cut(s) 23, 342, 357
Hin6I GCGC 3 cut(s) 21, 340, 355
HinP1I GCGC 3 cut(s) 21, 340, 355
HpaII CCGG 1 cut(s) 1385
HphI GGTGA 6 cut(s) 88, 280, 286, 495, 1129, 1567
Hpy166II GTNNAC 3 cut(s) 8, 1750, 1786
Hpy8I GTNNAC 3 cut(s) 8, 1750, 1786
Hpy99I CGWCG 1 cut(s) 756
HpyCH4III ACNGT 8 cut(s) 292, 400, 492, 1018, 1316, 1783, 1790, 2022
HpyCH4IV ACGT 4 cut(s) 58, 176, 751, 1101
HpyCH4V TGCA 9 cut(s) 8, 605, 664, 998, 1155, 1358, 1382, 1538, 1568
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 332, 602
HpySE526I ACGT 4 cut(s) 58, 176, 751, 1101
HspAI GCGC 3 cut(s) 21, 340, 355
Ksp22I TGATCA 1 cut(s) 165
Kzo9I GATC 6 cut(s) 165, 556, 611, 992, 1576, 1588
LmnI GCTCC 3 cut(s) 1324, 1531, 1796
Lsp1109I GCAGC 4 cut(s) 583, 1139, 1552, 1611
LweI GCATC 2 cut(s) 322, 2156
MaeI CTAG 5 cut(s) 27, 83, 383, 837, 1328
MaeII ACGT 4 cut(s) 58, 176, 751, 1101
MaeIII GTNAC 5 cut(s) 170, 292, 306, 794, 1135
MalI GATC 6 cut(s) 167, 558, 613, 994, 1578, 1590
MboI GATC 6 cut(s) 165, 556, 611, 992, 1576, 1588
MfeI CAATTG 2 cut(s) 300, 477
MflI RGATCY 3 cut(s) 556, 611, 1588
MhlI GDGCHC 4 cut(s) 10, 232, 533, 543
MlyI GAGTC 4 cut(s) 200, 314, 1420, 1982
MmeI TCCRAC 1 cut(s) 133
MseI TTAA 4 cut(s) 486, 764, 918, 1722
MslI CAYNNNNRTG 1 cut(s) 2085
MspI CCGG 1 cut(s) 1385
MspR9I CCNGG 1 cut(s) 71
MunI CAATTG 2 cut(s) 300, 477
Mva1269I GAATGC 1 cut(s) 1516
MvaI CCWGG 1 cut(s) 71
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 3 cut(s) 29, 332, 602
NdeII GATC 6 cut(s) 165, 556, 611, 992, 1576, 1588
NlaIV GGNNCC 3 cut(s) 909, 1033, 1666
NmuCI GTSAC 3 cut(s) 292, 306, 1135
NspI RCATGY 4 cut(s) 412, 1169, 1382, 2084
PaeI GCATGC 1 cut(s) 1169
PciI ACATGT 1 cut(s) 408
PcsI WCGNNNNNNNCGW 1 cut(s) 520
PctI GAATGC 1 cut(s) 1516
PfeI GAWTC 6 cut(s) 314, 932, 1214, 1634, 1640, 2090
PkrI GCNGC 5 cut(s) 573, 835, 1154, 1567, 1601
PleI GAGTC 4 cut(s) 199, 313, 1420, 1981
PmaCI CACGTG 1 cut(s) 59
PmlI CACGTG 1 cut(s) 59
PpsI GAGTC 4 cut(s) 199, 313, 1420, 1981
Ppu21I YACGTR 1 cut(s) 59
PscI ACATGT 1 cut(s) 408
Psp124BI GAGCTC 1 cut(s) 543
Psp1406I AACGTT 1 cut(s) 1101
Psp6I CCWGG 1 cut(s) 69
PspCI CACGTG 1 cut(s) 59
PspGI CCWGG 1 cut(s) 69
PspN4I GGNNCC 3 cut(s) 909, 1033, 1666
PspPI GGNCC 3 cut(s) 1007, 1094, 1861
PstI CTGCAG 2 cut(s) 1540, 1570
PsuI RGATCY 3 cut(s) 556, 611, 1588
RsaI GTAC 1 cut(s) 1785
RsaNI GTAC 1 cut(s) 1784
RseI CAYNNNNRTG 1 cut(s) 2085
SacI GAGCTC 1 cut(s) 543
SaqAI TTAA 4 cut(s) 486, 764, 918, 1722
SatI GCNGC 5 cut(s) 572, 834, 1153, 1566, 1600
Sau3AI GATC 6 cut(s) 165, 556, 611, 992, 1576, 1588
Sau96I GGNCC 3 cut(s) 1007, 1094, 1861
SchI GAGTC 4 cut(s) 200, 314, 1420, 1982
ScrFI CCNGG 1 cut(s) 71
SduI GDGCHC 4 cut(s) 10, 232, 533, 543
SfaNI GCATC 2 cut(s) 322, 2156
SfcI CTRYAG 3 cut(s) 1170, 1536, 1566
SinI GGWCC 3 cut(s) 1007, 1094, 1861
SmiMI CAYNNNNRTG 1 cut(s) 2085
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
SphI GCATGC 1 cut(s) 1169
SsiI CCGC 2 cut(s) 833, 1977
SspI AATATT 2 cut(s) 804, 1720
SspMI CTAG 5 cut(s) 27, 83, 383, 837, 1328
SstI GAGCTC 1 cut(s) 543
StyD4I CCNGG 1 cut(s) 69
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 8 cut(s) 292, 400, 492, 1018, 1316, 1783, 1790, 2022
TaiI ACGT 4 cut(s) 61, 179, 754, 1104
TaqI TCGA 6 cut(s) 523, 824, 967, 1277, 1394, 2001
TatI WGTACW 1 cut(s) 1783
TauI GCSGC 1 cut(s) 836
TfiI GAWTC 6 cut(s) 314, 932, 1214, 1634, 1640, 2090
Tru1I TTAA 4 cut(s) 486, 764, 918, 1722
Tru9I TTAA 4 cut(s) 486, 764, 918, 1722
TscAI CASTG 6 cut(s) 394, 538, 1321, 1354, 1761, 1793
TseFI GTSAC 3 cut(s) 292, 306, 1135
TseI GCWGC 4 cut(s) 571, 1152, 1565, 1599
Tsp45I GTSAC 3 cut(s) 292, 306, 1135
TspRI CASTG 6 cut(s) 394, 538, 1321, 1354, 1761, 1793
VneI GTGCAC 1 cut(s) 6
VpaK11BI GGWCC 3 cut(s) 1007, 1094, 1861
XapI RAATTY 7 cut(s) 104, 816, 1056, 1063, 1362, 1952, 2100
XceI RCATGY 4 cut(s) 412, 1169, 1382, 2084
XspI CTAG 5 cut(s) 27, 83, 383, 837, 1328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.