Rmu_sc0005946.1_g000003

At5g08430

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005946.1
OG0005324
Physical Location & Seq
Forward (+)
24196 .. 24864
669 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 669 bp
atgggctctttcacttgggttggggagtacaacgccgccgtcaccgtcaccgccaccgccgatgtcgacgccgccgccgctgctgctccgacgaggaggagcaagaggctgcaccgccccaagaagtttgagtttctcagctggggctcgaggccgctgattgaatttctcgagtcgattgggaaggacacgacgaagcagatctcccaatacgacgtcgctacgattatcaccgactacgtcaaccaccgcaggcttctccacccgacgaagaggaagaggatcgtctgcgacgaggggcttcattctctgttcggcaggaagaccattgctcggatcaagatctacgatttgctccagccgcatttcgctgagaatctcgaggacgattccgatgattcgtcggatgagtccgacgacgacggtttggacgaggaggacgaggataagcagcggcggcagcagcagcagcagcagaggaagaagcagcagaagaggagagccgccgcggctctgcatgcgcctcctccggttccgaagagctgctacgccgccgtgacaagcagcggcggcagcagctgcagcaaaggaagaagcagcagaagaggagagccgccgcggctctgcatgcgcctcctccggttccgaagagctgctacgccgccgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF Plus-3 SWIB

Protein Analysis

222

Amino Acids

25.05

Weight (kDa)

9.51

Isoelectric Point (pI)

72.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIB PF02201 50 - 123 5.4e-10 SWIB/MDM2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 219
AccI GTMKAC 1 cut(s) 66
AccII CGCG 2 cut(s) 509, 619
AclWI GGATC 2 cut(s) 290, 344
AcsI RAATTY 1 cut(s) 164
AcyI GRCGYC 2 cut(s) 69, 216
AfaI GTAC 1 cut(s) 29
AfiI CCNNNNNNNGG 1 cut(s) 333
AgsI TTSAA 1 cut(s) 164
AluBI AGCT 4 cut(s) 141, 543, 579, 653
AluI AGCT 4 cut(s) 141, 543, 579, 653
AlwI GGATC 2 cut(s) 290, 344
AlwNI CAGNNNCTG 1 cut(s) 579
Ama87I CYCGRG 3 cut(s) 148, 170, 380
AoxI GGCC 1 cut(s) 152
ApoI RAATTY 1 cut(s) 164
AspLEI GCGC 2 cut(s) 523, 633
AsuHPI GGTGA 3 cut(s) 34, 40, 223
AvaI CYCGRG 3 cut(s) 148, 170, 380
BanII GRGCYC 2 cut(s) 8, 149
BarI GAAGNNNNNNTAC 3 cut(s) 530, 562, 640
BbsI GAAGAC 1 cut(s) 329
BceAI ACGGC 3 cut(s) 23, 539, 649
BfmI CTRYAG 1 cut(s) 580
BglI GCCNNNNNGGC 2 cut(s) 509, 619
BglII AGATCT 2 cut(s) 201, 342
BmeT110I CYCGRG 3 cut(s) 148, 170, 380
BmiI GGNNCC 2 cut(s) 534, 644
BpiI GAAGAC 1 cut(s) 329
BpmI CTGGAG 1 cut(s) 341
BsaBI GATNNNNATC 1 cut(s) 341
BsaHI GRCGYC 2 cut(s) 69, 216
BsaJI CCNNGG 2 cut(s) 507, 617
BsaWI WCCGGW 2 cut(s) 529, 639
Bsc4I CCNNNNNNNGG 1 cut(s) 333
Bse3DI GCAATG 1 cut(s) 327
Bse8I GATNNNNATC 1 cut(s) 341
BseDI CCNNGG 2 cut(s) 507, 617
BseGI GGATG 1 cut(s) 412
BseJI GATNNNNATC 1 cut(s) 341
BseLI CCNNNNNNNGG 1 cut(s) 333
BseMI GCAATG 1 cut(s) 327
BseMII CTCAG 2 cut(s) 151, 363
BseRI GAGGAG 7 cut(s) 109, 112, 449, 511, 516, 621, 626
BseYI CCCAGC 1 cut(s) 141
BsgI GTGCAG 1 cut(s) 95
Bsh1236I CGCG 2 cut(s) 509, 619
BshFI GGCC 1 cut(s) 154
BsiHKCI CYCGRG 3 cut(s) 148, 170, 380
BsiSI CCGG 2 cut(s) 530, 640
BslI CCNNNNNNNGG 1 cut(s) 333
BsnI GGCC 1 cut(s) 154
BsoBI CYCGRG 3 cut(s) 148, 170, 380
Bsp1286I GDGCHC 2 cut(s) 8, 149
Bsp143I GATC 4 cut(s) 201, 282, 336, 342
BspANI GGCC 1 cut(s) 154
BspCNI CTCAG 2 cut(s) 150, 364
BspFNI CGCG 2 cut(s) 509, 619
BspLI GGNNCC 2 cut(s) 534, 644
BspMAI CTGCAG 1 cut(s) 584
BspPI GGATC 2 cut(s) 290, 344
BspQI GCTCTTC 2 cut(s) 533, 643
BsrDI GCAATG 1 cut(s) 327
BssECI CCNNGG 2 cut(s) 507, 617
BssMI GATC 4 cut(s) 201, 282, 336, 342
BssNI GRCGYC 2 cut(s) 69, 216
Bst4CI ACNGT 2 cut(s) 46, 425
Bst6I CTCTTC 6 cut(s) 266, 272, 488, 533, 598, 643
BstACI GRCGYC 2 cut(s) 69, 216
BstAPI GCANNNNNTGC 1 cut(s) 579
BstC8I GCNNGC 3 cut(s) 254, 519, 629
BstDEI CTNAG 2 cut(s) 137, 372
BstDSI CCRYGG 2 cut(s) 507, 617
BstF5I GGATG 1 cut(s) 412
BstFNI CGCG 2 cut(s) 509, 619
BstHHI GCGC 2 cut(s) 523, 633
BstKTI GATC 4 cut(s) 204, 285, 339, 345
BstMBI GATC 4 cut(s) 201, 282, 336, 342
BstNSI RCATGY 2 cut(s) 521, 631
BstSFI CTRYAG 1 cut(s) 580
BstUI CGCG 2 cut(s) 509, 619
BstV2I GAAGAC 1 cut(s) 329
BstX2I RGATCY 2 cut(s) 201, 342
BstYI RGATCY 2 cut(s) 201, 342
BsuRI GGCC 1 cut(s) 154
BtgI CCRYGG 2 cut(s) 507, 617
BtsCI GGATG 1 cut(s) 412
Cac8I GCNNGC 3 cut(s) 254, 519, 629
CaiI CAGNNNCTG 1 cut(s) 579
CfoI GCGC 2 cut(s) 523, 633
Cfr42I CCGCGG 2 cut(s) 510, 620
CseI GACGC 1 cut(s) 77
Csp6I GTAC 1 cut(s) 28
CviAII CATG 2 cut(s) 518, 628
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 2 cut(s) 137, 372
DpnI GATC 4 cut(s) 203, 284, 338, 344
DpnII GATC 4 cut(s) 201, 282, 336, 342
Eam1104I CTCTTC 6 cut(s) 266, 272, 488, 533, 598, 643
EarI CTCTTC 6 cut(s) 266, 272, 488, 533, 598, 643
Eco24I GRGCYC 2 cut(s) 8, 149
Eco88I CYCGRG 3 cut(s) 148, 170, 380
EcoT38I GRGCYC 2 cut(s) 8, 149
FaeI CATG 2 cut(s) 521, 631
FaiI YATR 2 cut(s) 519, 629
FatI CATG 2 cut(s) 517, 627
FblI GTMKAC 1 cut(s) 66
FokI GGATG 1 cut(s) 419
FriOI GRGCYC 2 cut(s) 8, 149
GlaI GCGC 2 cut(s) 522, 632
GsaI CCCAGC 1 cut(s) 145
GsuI CTGGAG 1 cut(s) 341
HaeIII GGCC 1 cut(s) 154
HapII CCGG 2 cut(s) 530, 640
HgaI GACGC 1 cut(s) 77
HhaI GCGC 2 cut(s) 523, 633
Hin1I GRCGYC 2 cut(s) 69, 216
Hin1II CATG 2 cut(s) 521, 631
Hin6I GCGC 2 cut(s) 521, 631
HinP1I GCGC 2 cut(s) 521, 631
HincII GTYRAC 2 cut(s) 67, 244
HindII GTYRAC 2 cut(s) 67, 244
HinfI GANTC 5 cut(s) 173, 376, 389, 398, 410
HpaII CCGG 2 cut(s) 530, 640
HphI GGTGA 3 cut(s) 34, 40, 223
Hpy166II GTNNAC 2 cut(s) 67, 244
Hpy188I TCNGA 7 cut(s) 90, 336, 394, 406, 415, 537, 647
Hpy188III TCNNGA 3 cut(s) 170, 340, 380
Hpy8I GTNNAC 2 cut(s) 67, 244
HpyAV CCTTC 1 cut(s) 178
HpyCH4III ACNGT 2 cut(s) 46, 425
HpyCH4IV ACGT 2 cut(s) 216, 240
HpyCH4V TGCA 4 cut(s) 112, 517, 582, 627
HpyF3I CTNAG 2 cut(s) 137, 372
HpySE526I ACGT 2 cut(s) 216, 240
Hsp92I GRCGYC 2 cut(s) 69, 216
Hsp92II CATG 2 cut(s) 521, 631
HspAI GCGC 2 cut(s) 521, 631
KspI CCGCGG 2 cut(s) 510, 620
Kzo9I GATC 4 cut(s) 201, 282, 336, 342
LguI GCTCTTC 2 cut(s) 533, 643
LmnI GCTCC 3 cut(s) 91, 99, 360
LpnPI CCDG 6 cut(s) 127, 238, 304, 371, 543, 653
MaeII ACGT 2 cut(s) 216, 240
MaeIII GTNAC 3 cut(s) 40, 46, 556
MalI GATC 4 cut(s) 203, 284, 338, 344
MboI GATC 4 cut(s) 201, 282, 336, 342
MboII GAAGA 9 cut(s) 283, 289, 334, 493, 505, 550, 603, 615, 660
MflI RGATCY 2 cut(s) 201, 342
MhlI GDGCHC 2 cut(s) 8, 149
MluCI AATT 1 cut(s) 164
MlyI GAGTC 2 cut(s) 182, 419
MmeI TCCRAC 3 cut(s) 113, 384, 438
MspA1I CMGCKG 8 cut(s) 80, 141, 157, 454, 509, 567, 579, 619
MspI CCGG 2 cut(s) 530, 640
MvnI CGCG 2 cut(s) 509, 619
NdeII GATC 4 cut(s) 201, 282, 336, 342
NlaIII CATG 2 cut(s) 521, 631
NlaIV GGNNCC 2 cut(s) 534, 644
NmuCI GTSAC 3 cut(s) 40, 46, 556
NspI RCATGY 2 cut(s) 521, 631
PaeI GCATGC 2 cut(s) 521, 631
PaeR7I CTCGAG 3 cut(s) 148, 170, 380
PciSI GCTCTTC 2 cut(s) 533, 643
PcsI WCGNNNNNNNCGW 3 cut(s) 291, 429, 438
PfeI GAWTC 3 cut(s) 376, 389, 398
PflFI GACNNNGTC 1 cut(s) 239
PleI GAGTC 2 cut(s) 181, 418
PpsI GAGTC 2 cut(s) 181, 418
PspFI CCCAGC 1 cut(s) 141
PspN4I GGNNCC 2 cut(s) 534, 644
PspXI VCTCGAGB 1 cut(s) 148
PstI CTGCAG 1 cut(s) 584
PstNI CAGNNNCTG 1 cut(s) 579
PsuI RGATCY 2 cut(s) 201, 342
PsyI GACNNNGTC 1 cut(s) 239
PvuII CAGCTG 2 cut(s) 141, 579
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
SacII CCGCGG 2 cut(s) 510, 620
SalI GTCGAC 1 cut(s) 65
SapI GCTCTTC 2 cut(s) 533, 643
Sau3AI GATC 4 cut(s) 201, 282, 336, 342
SchI GAGTC 2 cut(s) 182, 419
SduI GDGCHC 2 cut(s) 8, 149
SetI ASST 6 cut(s) 143, 219, 243, 545, 581, 655
SfcI CTRYAG 1 cut(s) 580
Sfr274I CTCGAG 3 cut(s) 148, 170, 380
Sfr303I CCGCGG 2 cut(s) 510, 620
SgrBI CCGCGG 2 cut(s) 510, 620
SlaI CTCGAG 3 cut(s) 148, 170, 380
SmlI CTYRAG 3 cut(s) 148, 170, 380
SmoI CTYRAG 3 cut(s) 148, 170, 380
SphI GCATGC 2 cut(s) 521, 631
Sse9I AATT 1 cut(s) 164
TaaI ACNGT 2 cut(s) 46, 425
TaiI ACGT 2 cut(s) 219, 243
TaqI TCGA 5 cut(s) 66, 149, 171, 176, 381
TasI AATT 1 cut(s) 164
TatI WGTACW 1 cut(s) 27
TfiI GAWTC 3 cut(s) 376, 389, 398
TseFI GTSAC 3 cut(s) 40, 46, 556
Tsp45I GTSAC 3 cut(s) 40, 46, 556
TspDTI ATGAA 1 cut(s) 293
Tth111I GACNNNGTC 1 cut(s) 239
XapI RAATTY 1 cut(s) 164
XceI RCATGY 2 cut(s) 521, 631
XhoI CTCGAG 3 cut(s) 148, 170, 380
XmiI GTMKAC 1 cut(s) 66
ZraI GACGTC 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.