Rmu_sc0006153.1_g000056

Nucleolar protein 6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006153.1
OG0008672
Physical Location & Seq
Reverse (-)
197565 .. 207906
10342 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3528 bp
atggaaaccgataactcagcggacctgaaggtggaggagctcttgaaagaggtccagctagaatattcacctgctctcaccaagttcgtagacgatgccgtttcatcgatcaaagcagccatcgatacaattccacaagacctcaaggttacggctgatttagctccgggattcgtcagagatattggtgcagacaaagttgaatttgatttcaagaagccgaaatctgtcaaaattggcggcagttatgctatagagtgccttgtaaagcctgagttcaatgtggatcttttcgttcagttgcccaaggaatgtttccatgagaaagactatttgaattaccggtaccatgctaaaaggtgcctgtacctttgtgtgattaaaaagtacttgatgtcgtcttcgttagttggtaaggttgaatggtccactttccagaacgaagtccggaaacctgtgttgattgtgtatccagtcacgaaattagttgcactccctgggttctttgtgagaataattcccacagcaccatctctttttagcattccaaaattaaacctgcagcggaataatgttcgtgctgtgagtaaagggggcattcctcagtctactcccaagtacaatagtagtattttggaagacatgtttatagaagatacagaagaacttgtcaagcaaatttttcttgggtcaaaggaattacgacaaggtttagttttgctgaaggtctgggctcgccgcaggatgccaatatatgcttatgattgcctgaatgggtttctaatctctgtcatattggcataccttgtggataggaaccatatcaataaatcaatgaaggcaatgcaaatatttcgtgttacaatgaaattcattgctacttcagatttgtggaaacatgggctctacttcacaccaaaaggccaaaaagctatttcaaaagaggagaggttgccttttaaagaatcatttccaattgttatatgtactccatctagaagctttaatttagccttccgggtaacaagggttggatttctcgagctgcaaaatgaatctacaatgacactggcatgcatagagaaatgcagagatagtggatttgaggaagtatttatgaccaggattgactatcctgtaaaatatgaccatgtcataaggttgaacttaaagaggaagagttcagtttatggattgggattttgcttggatgatgaatgttggagagtgtatgagcagaaggttcataatgttttaagtcatgggttgagtgaccgagtgaagactgtacgtgtgacctggaaaagcatgttatcagaatccattctacaaaatggattgtcgtcactcaatacagagccactgctaattggaatttcagtgagctctttagataaagctttaagaattgttgatattggtcctgatgctgataataaggatgaggctctcaaatttcgtcagttctggggggataaggctgagcttaggagattcaaagatggcaagatagcagaaagcacagtttgggaaactgagcaatggaaaagacatatcgtcataaaaaagatatctgagcatgtgcttctgcgacatctttcactatcgaaagagaatatcatgcatatcgtggatcaacttgatttctctttactatatggtgccgaagatccgatatcttcttccggaagtttgtttggggcgtttgaaattctatcgaagcgattgcgccttattgaagacattcctctaaaggtttctacagtgcaggccctagattcggctttcaggtattcatctgtcttcccacctgaacctcatccgctagctaatgaaaagggttctttcgtaagactaaatcagttccctccttcctgcatccggccgctggaagttatgatccagttggaaggttctggaaactggcctatggatgatgtagccattgagaaaactaaatcagcattccttcttaaaatcggagagagtcttcagaatagttgggggatgacatgtacagccactgaggatgatgtggatgtttttgtctctggatatgcctttcgtcttaaaatttggcatgaaagaggcctgactttgttgagacgtgaaactgggaatgaacacgtgaagaaggtttctaatgtggacaaggaactatactttcgcagtcaacattccagcatgataaatggattccagacttgttacccagcatatgggccagttgttaggcttgcaaaacgatgggctgcttcacatctattttcggcttgcttggtagaggaggcaattgagctactggtagcatatatatttttgaagcctttaccatttaatgctccatgctcacgaatcactggatttttaaggttcctgcgattactatcagattatgactggaccttttctgcgttagttgttgacataaataatgatttaacatcaaaggatgagaaagagataagagagaacttcatgttaagtagaaaaggttatgaagaaaatcctcaaaatgtaaactcagcaatgttcctagctacatcctacgacaatgcatctgaggcttggaccaaattttcaccaaattcaatggagctaaaaaggttgatggcttatgctggaagcagtgcaaacttgttgaccaaactgatattggaggaccatagtgattcttatagatgggagtgcctttttcgaactcctttgaacaattatgatgctgtaattcttctccatagagaaaaattaccttatcctcaacgccttctctttccatcagaattacatcaaggcgttcatgtggcacgtgggaatgcaagcaagggtttccacccctttcttttgcctggagattttaaaggaagctcagaggatctaagaaacaaagtattggtaaacttcgatccgctaaggtgttttgttggggatctagagaaagagtactcaaacactttcaagctatggtatgattgtctgggtggtgatgctgttggaataacatggggaggatgtatttcaaagaaacggggccgggaagaagcagatgaggaagtaaaggatccaattgatttgttaaaagatgttggtaaagttggaacagggcggcagagtgattggagtactgcttcgtcgtggggcagcgttcgaccctatggggaatctgggcgaggcaagggcttcatcttcttctccatttccttgcggtcttccgacggcgatagtggcacaactttcggggatggagcaactggtatgagtcttgggttaatgaggacggagtctcgggtcttcgatggtggaggcggaattttgccttgggttgttggcataggggtcgtgctggtggccaccggaggcgcttgggacggagatggaggatgtatagaggtcagcagtggtggtgacaactgtgaggaagctggacgccgcgacaaagcttgggcggtggctgacgtgtggaagctgggcattgtgctcctagggtttgaaggtgacgtgtcatcttctagggctagcttaatttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
Nrap Nrap_D2 Nrap_D3 Nrap_D4 Nrap_D5 Nrap_D6

Protein Analysis

1175

Amino Acids

132.42

Weight (kDa)

7.26

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nrap PF03813 95 - 227 1.1e-34 Nrap protein domain 1
Nrap_D2 PF17403 234 - 375 4.9e-31 Nrap protein PAP/OAS-like domain
Nrap_D3 PF17404 380 - 534 2e-37 Nrap protein domain 3
Nrap_D4 PF17405 556 - 735 3.5e-45 Nrap protein nucleotidyltransferase domain 4
Nrap_D5 PF17406 737 - 889 8.8e-47 Nrap protein PAP/OAS1-like domain 5
Nrap_D6 PF17407 903 - 1008 8.1e-12 Nrap protein domain 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 79
Acc36I ACCTGC 2 cut(s) 79, 567
Acc65I GGTACC 1 cut(s) 345
AccB1I GGYRCC 3 cut(s) 345, 360, 1661
AccB7I CCANNNNNTGG 1 cut(s) 2219
AccI GTMKAC 2 cut(s) 90, 608
AccII CGCG 1 cut(s) 3432
AccIII TCCGGA 2 cut(s) 447, 1685
AclWI GGATC 9 cut(s) 294, 1641, 1664, 1894, 2883, 2900, 2937, 3056, 3069
AcoI YGGCCR 2 cut(s) 1883, 3348
AcuI CTGAAG 4 cut(s) 47, 743, 867, 1976
AcvI CACGTG 2 cut(s) 2128, 2810
AcyI GRCGYC 1 cut(s) 3427
AfaI GTAC 9 cut(s) 347, 368, 389, 620, 988, 1290, 2017, 2945, 3124
AfiI CCNNNNNNNGG 5 cut(s) 31, 1780, 1881, 1888, 2219
AflIII ACRYGT 6 cut(s) 642, 1291, 2012, 2125, 3456, 3498
AgeI ACCGGT 1 cut(s) 342
AhdI GACNNNNNGTC 1 cut(s) 1556
AjiI CACGTC 3 cut(s) 2108, 3457, 3499
AjnI CCWGG 4 cut(s) 496, 1121, 1298, 2848
AjuI GAANNNNNNNTTGG 4 cut(s) 299, 331, 3301, 3333
Alw21I GWGCWC 3 cut(s) 42, 1388, 3480
Alw26I GTCTC 3 cut(s) 2053, 2098, 3288
AlwI GGATC 9 cut(s) 294, 1641, 1664, 1894, 2883, 2900, 2937, 3056, 3069
AlwNI CAGNNNCTG 1 cut(s) 2024
Ama87I CYCGRG 2 cut(s) 1040, 3285
Aor13HI TCCGGA 2 cut(s) 447, 1685
AoxI GGCC 8 cut(s) 922, 1770, 1883, 1925, 2089, 2222, 3031, 3348
ApeKI GCWGC 5 cut(s) 116, 562, 1045, 2252, 3141
AsiGI ACCGGT 1 cut(s) 342
Asp700I GAANNNNTTC 3 cut(s) 969, 1323, 1743
Asp718I GGTACC 1 cut(s) 345
AspA2I CCTAGG 1 cut(s) 3481
AspLEI GCGC 2 cut(s) 1731, 3362
AsuC2I CCSGG 3 cut(s) 168, 1019, 3035
AsuHPI GGTGA 6 cut(s) 60, 70, 2574, 2996, 3416, 3506
AsuII TTCGAA 1 cut(s) 2698
AsuNHI GCTAGC 2 cut(s) 1825, 3515
AvaI CYCGRG 2 cut(s) 1040, 3285
AvaII GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
AvrII CCTAGG 1 cut(s) 3481
BalI TGGCCA 1 cut(s) 3350
BamHI GGATCC 1 cut(s) 3061
BanI GGYRCC 3 cut(s) 345, 360, 1661
BanII GRGCYC 4 cut(s) 42, 736, 906, 1388
BbrPI CACGTG 2 cut(s) 2128, 2810
BbsI GAAGAC 8 cut(s) 393, 645, 1289, 1746, 1795, 1982, 3201, 3283
Bbv12I GWGCWC 3 cut(s) 42, 1388, 3480
BbvI GCAGC 5 cut(s) 128, 574, 1032, 2239, 3153
BceAI ACGGC 3 cut(s) 83, 168, 3232
BcgI CGANNNNNNTGC 8 cut(s) 836, 870, 1708, 1742, 3217, 3251, 3319, 3353
BciT130I CCWGG 4 cut(s) 498, 1123, 1300, 2850
BciVI GTATCC 1 cut(s) 480
BcnI CCSGG 3 cut(s) 168, 1019, 3035
BcoDI GTCTC 3 cut(s) 2053, 2098, 3288
BfaI CTAG 9 cut(s) 59, 996, 1775, 1826, 2537, 2933, 3482, 3510, 3516
BfmI CTRYAG 3 cut(s) 252, 560, 1761
BfoI RGCGCY 1 cut(s) 3363
BfuAI ACCTGC 2 cut(s) 79, 567
BfuI GTATCC 1 cut(s) 480
BlnI CCTAGG 1 cut(s) 3481
BlpI GCTNAGC 1 cut(s) 1482
BmcAI AGTACT 3 cut(s) 389, 2945, 3124
Bme1390I CCNGG 7 cut(s) 168, 498, 1019, 1123, 1300, 2850, 3035
Bme18I GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
BmeRI GACNNNNNGTC 1 cut(s) 1556
BmeT110I CYCGRG 2 cut(s) 1040, 3285
BmgBI CACGTC 3 cut(s) 2108, 3457, 3499
BmiI GGNNCC 7 cut(s) 347, 362, 818, 1663, 2375, 3032, 3063
BmrFI CCNGG 7 cut(s) 168, 498, 1019, 1123, 1300, 2850, 3035
BmrI ACTGGG 1 cut(s) 2124
BmsI GCATC 7 cut(s) 85, 735, 1417, 1887, 2567, 2710, 2977
BmtI GCTAGC 2 cut(s) 1829, 3519
BmuI ACTGGG 1 cut(s) 2124
BpiI GAAGAC 8 cut(s) 393, 645, 1289, 1746, 1795, 1982, 3201, 3283
BpmI CTGGAG 1 cut(s) 2871
Bpu10I CCTNAGC 2 cut(s) 1487, 2912
Bpu1102I GCTNAGC 1 cut(s) 1482
Bpu14I TTCGAA 1 cut(s) 2698
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 3 cut(s) 168, 1019, 3035
Bsa29I ATCGAT 2 cut(s) 107, 123
BsaAI YACGTR 3 cut(s) 1292, 2128, 2810
BsaBI GATNNNNATC 1 cut(s) 2386
BsaHI GRCGYC 1 cut(s) 3427
BsaJI CCNNGG 5 cut(s) 306, 496, 497, 3317, 3481
BsaWI WCCGGW 4 cut(s) 342, 447, 1685, 3353
Bsc4I CCNNNNNNNGG 5 cut(s) 31, 1780, 1881, 1888, 2219
Bse118I RCCGGY 1 cut(s) 342
Bse3DI GCAATG 4 cut(s) 849, 873, 1547, 2535
Bse8I GATNNNNATC 1 cut(s) 2386
BseAI TCCGGA 2 cut(s) 447, 1685
BseBI CCWGG 4 cut(s) 498, 1123, 1300, 2850
BseCI ATCGAT 2 cut(s) 107, 123
BseDI CCNNGG 5 cut(s) 306, 496, 497, 3317, 3481
BseJI GATNNNNATC 1 cut(s) 2386
BseLI CCNNNNNNNGG 5 cut(s) 31, 1780, 1881, 1888, 2219
BseMI GCAATG 4 cut(s) 849, 873, 1547, 2535
BseRI GAGGAG 3 cut(s) 50, 959, 2300
BseX3I CGGCCG 1 cut(s) 1883
BseXI GCAGC 5 cut(s) 128, 574, 1032, 2239, 3153
BseYI CCCAGC 2 cut(s) 2212, 3466
BsgI GTGCAG 2 cut(s) 210, 1787
Bsh1236I CGCG 1 cut(s) 3432
Bsh1285I CGRYCG 1 cut(s) 1886
BshFI GGCC 8 cut(s) 924, 1772, 1885, 1927, 2091, 2224, 3033, 3350
BshNI GGYRCC 3 cut(s) 345, 360, 1661
BshTI ACCGGT 1 cut(s) 342
BshVI ATCGAT 2 cut(s) 107, 123
BsiEI CGRYCG 1 cut(s) 1886
BsiHKAI GWGCWC 3 cut(s) 42, 1388, 3480
BsiHKCI CYCGRG 2 cut(s) 1040, 3285
BsiSI CCGG 8 cut(s) 167, 343, 448, 1018, 1686, 1882, 3034, 3354
BslFI GGGAC 1 cut(s) 3380
BslI CCNNNNNNNGG 5 cut(s) 31, 1780, 1881, 1888, 2219
BsmAI GTCTC 3 cut(s) 2053, 2098, 3288
BsmBI CGTCTC 1 cut(s) 2098
BsmFI GGGAC 1 cut(s) 3380
BsmI GAATGC 4 cut(s) 543, 597, 1964, 2821
BsnI GGCC 8 cut(s) 924, 1772, 1885, 1927, 2091, 2224, 3033, 3350
BsoBI CYCGRG 2 cut(s) 1040, 3285
Bsp119I TTCGAA 1 cut(s) 2698
Bsp1286I GDGCHC 5 cut(s) 42, 736, 906, 1388, 3480
Bsp13I TCCGGA 2 cut(s) 447, 1685
Bsp1407I TGTACA 1 cut(s) 2015
Bsp143I GATC 9 cut(s) 108, 286, 1633, 1669, 1899, 2875, 2905, 2929, 3061
Bsp1720I GCTNAGC 1 cut(s) 1482
BspANI GGCC 8 cut(s) 924, 1772, 1885, 1927, 2091, 2224, 3033, 3350
BspDI ATCGAT 2 cut(s) 107, 123
BspEI TCCGGA 2 cut(s) 447, 1685
BspFNI CGCG 1 cut(s) 3432
BspLI GGNNCC 7 cut(s) 347, 362, 818, 1663, 2375, 3032, 3063
BspMAI CTGCAG 1 cut(s) 564
BspMI ACCTGC 2 cut(s) 79, 567
BspOI GCTAGC 2 cut(s) 1829, 3519
BspPI GGATC 9 cut(s) 294, 1641, 1664, 1894, 2883, 2900, 2937, 3056, 3069
BspT104I TTCGAA 1 cut(s) 2698
BspT107I GGYRCC 3 cut(s) 345, 360, 1661
BsrDI GCAATG 4 cut(s) 849, 873, 1547, 2535
BsrFI RCCGGY 1 cut(s) 342
BsrGI TGTACA 1 cut(s) 2015
BssAI RCCGGY 1 cut(s) 342
BssECI CCNNGG 5 cut(s) 306, 496, 497, 3317, 3481
BssMI GATC 9 cut(s) 108, 286, 1633, 1669, 1899, 2875, 2905, 2929, 3061
BssNI GRCGYC 1 cut(s) 3427
BssT1I CCWWGG 3 cut(s) 306, 3317, 3481
Bst2UI CCWGG 4 cut(s) 498, 1123, 1300, 2850
Bst4CI ACNGT 4 cut(s) 1288, 1525, 1765, 3413
Bst6I CTCTTC 1 cut(s) 1172
BstACI GRCGYC 1 cut(s) 3427
BstAUI TGTACA 1 cut(s) 2015
BstBAI YACGTR 3 cut(s) 1292, 2128, 2810
BstBI TTCGAA 1 cut(s) 2698
BstC8I GCNNGC 8 cut(s) 736, 1075, 1770, 1827, 2238, 2275, 2821, 3517
BstFNI CGCG 1 cut(s) 3432
BstH2I RGCGCY 1 cut(s) 3363
BstHHI GCGC 2 cut(s) 1731, 3362
BstKTI GATC 9 cut(s) 111, 289, 1636, 1672, 1902, 2878, 2908, 2932, 3064
BstMAI GTCTC 3 cut(s) 2053, 2098, 3288
BstMBI GATC 9 cut(s) 108, 286, 1633, 1669, 1899, 2875, 2905, 2929, 3061
BstMCI CGRYCG 1 cut(s) 1886
BstMWI GCNNNNNNNGC 3 cut(s) 161, 2564, 3225
BstNI CCWGG 4 cut(s) 498, 1123, 1300, 2850
BstNSI RCATGY 5 cut(s) 646, 1077, 1312, 1583, 2016
BstSCI CCNGG 7 cut(s) 166, 496, 1017, 1121, 1298, 2848, 3033
BstSFI CTRYAG 3 cut(s) 252, 560, 1761
BstUI CGCG 1 cut(s) 3432
BstV1I GCAGC 5 cut(s) 128, 574, 1032, 2239, 3153
BstV2I GAAGAC 8 cut(s) 393, 645, 1289, 1746, 1795, 1982, 3201, 3283
BstX2I RGATCY 5 cut(s) 286, 1669, 2875, 2929, 3061
BstYI RGATCY 5 cut(s) 286, 1669, 2875, 2929, 3061
BstZI CGGCCG 1 cut(s) 1883
Bsu15I ATCGAT 2 cut(s) 107, 123
BsuI GTATCC 1 cut(s) 480
BsuRI GGCC 8 cut(s) 924, 1772, 1885, 1927, 2091, 2224, 3033, 3350
BsuTUI ATCGAT 2 cut(s) 107, 123
BtrI CACGTC 3 cut(s) 2108, 3457, 3499
BtsI GCAGTG 3 cut(s) 1361, 2635, 3403
BtsIMutI CAGTG 8 cut(s) 1067, 1361, 1386, 1770, 2022, 2358, 2635, 3403
BveI ACCTGC 2 cut(s) 79, 567
Cac8I GCNNGC 8 cut(s) 736, 1075, 1770, 1827, 2238, 2275, 2821, 3517
CaiI CAGNNNCTG 1 cut(s) 2024
CfoI GCGC 2 cut(s) 1731, 3362
Cfr10I RCCGGY 1 cut(s) 342
ClaI ATCGAT 2 cut(s) 107, 123
CseI GACGC 1 cut(s) 3435
Csp6I GTAC 9 cut(s) 346, 367, 388, 619, 987, 1289, 2016, 2944, 3123
CspAI ACCGGT 1 cut(s) 342
CviQI GTAC 9 cut(s) 346, 367, 388, 619, 987, 1289, 2016, 2944, 3123
DpnI GATC 9 cut(s) 110, 288, 1635, 1671, 1901, 2877, 2907, 2931, 3063
DpnII GATC 9 cut(s) 108, 286, 1633, 1669, 1899, 2875, 2905, 2929, 3061
DraI TTTAAA 2 cut(s) 961, 2860
DriI GACNNNNNGTC 1 cut(s) 1556
EaeI YGGCCR 2 cut(s) 1883, 3348
EagI CGGCCG 1 cut(s) 1883
Eam1104I CTCTTC 1 cut(s) 1172
Eam1105I GACNNNNNGTC 1 cut(s) 1556
EarI CTCTTC 1 cut(s) 1172
EciI GGCGGA 1 cut(s) 3321
Ecl136II GAGCTC 2 cut(s) 40, 1386
EclXI CGGCCG 1 cut(s) 1883
Eco130I CCWWGG 3 cut(s) 306, 3317, 3481
Eco147I AGGCCT 1 cut(s) 2091
Eco24I GRGCYC 4 cut(s) 42, 736, 906, 1388
Eco32I GATATC 2 cut(s) 1572, 1677
Eco47I GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
Eco52I CGGCCG 1 cut(s) 1883
Eco53kI GAGCTC 2 cut(s) 40, 1386
Eco57I CTGAAG 4 cut(s) 47, 743, 867, 1976
Eco72I CACGTG 2 cut(s) 2128, 2810
Eco88I CYCGRG 2 cut(s) 1040, 3285
EcoICRI GAGCTC 2 cut(s) 40, 1386
EcoO109I RGGNCCY 1 cut(s) 1771
EcoRII CCWGG 4 cut(s) 496, 1121, 1298, 2848
EcoRV GATATC 2 cut(s) 1572, 1677
EcoT14I CCWWGG 3 cut(s) 306, 3317, 3481
EcoT22I ATGCAT 3 cut(s) 1079, 1626, 2560
EcoT38I GRGCYC 4 cut(s) 42, 736, 906, 1388
ErhI CCWWGG 3 cut(s) 306, 3317, 3481
Esp3I CGTCTC 1 cut(s) 2098
FaqI GGGAC 1 cut(s) 3380
FauNDI CATATG 1 cut(s) 2218
FblI GTMKAC 2 cut(s) 90, 608
FriOI GRGCYC 4 cut(s) 42, 736, 906, 1388
FspBI CTAG 9 cut(s) 59, 996, 1775, 1826, 2537, 2933, 3482, 3510, 3516
GlaI GCGC 2 cut(s) 1730, 3361
GsaI CCCAGC 2 cut(s) 2216, 3470
GsuI CTGGAG 1 cut(s) 2871
HaeII RGCGCY 1 cut(s) 3363
HaeIII GGCC 8 cut(s) 924, 1772, 1885, 1927, 2091, 2224, 3033, 3350
HapII CCGG 8 cut(s) 167, 343, 448, 1018, 1686, 1882, 3034, 3354
HgaI GACGC 1 cut(s) 3435
HhaI GCGC 2 cut(s) 1731, 3362
Hin1I GRCGYC 1 cut(s) 3427
Hin6I GCGC 2 cut(s) 1729, 3360
HinP1I GCGC 2 cut(s) 1729, 3360
HincII GTYRAC 3 cut(s) 2174, 2425, 2643
HindII GTYRAC 3 cut(s) 2174, 2425, 2643
HindIII AAGCTT 3 cut(s) 1000, 1398, 3438
HpaII CCGG 8 cut(s) 167, 343, 448, 1018, 1686, 1882, 3034, 3354
HphI GGTGA 6 cut(s) 60, 70, 2574, 2996, 3416, 3506
Hpy166II GTNNAC 9 cut(s) 91, 429, 609, 2149, 2174, 2425, 2521, 2643, 2899
Hpy8I GTNNAC 9 cut(s) 91, 429, 609, 2149, 2174, 2425, 2521, 2643, 2899
Hpy99I CGWCG 2 cut(s) 3136, 3218
HpyCH4III ACNGT 4 cut(s) 1288, 1525, 1765, 3413
HpyCH4IV ACGT 6 cut(s) 1291, 2107, 2127, 2809, 3456, 3498
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 2564, 3225
HpySE526I ACGT 6 cut(s) 1291, 2107, 2127, 2809, 3456, 3498
Hsp92I GRCGYC 1 cut(s) 3427
HspAI GCGC 2 cut(s) 1729, 3360
Kpn2I TCCGGA 2 cut(s) 447, 1685
KpnI GGTACC 1 cut(s) 349
Kzo9I GATC 9 cut(s) 108, 286, 1633, 1669, 1899, 2875, 2905, 2929, 3061
LmnI GCTCC 6 cut(s) 37, 169, 2347, 2596, 3245, 3483
Lsp1109I GCAGC 5 cut(s) 128, 574, 1032, 2239, 3153
LweI GCATC 7 cut(s) 85, 735, 1417, 1887, 2567, 2710, 2977
MaeI CTAG 9 cut(s) 59, 996, 1775, 1826, 2537, 2933, 3482, 3510, 3516
MaeII ACGT 6 cut(s) 1291, 2107, 2127, 2809, 3456, 3498
MalI GATC 9 cut(s) 110, 288, 1635, 1671, 1901, 2877, 2907, 2931, 3063
MboI GATC 9 cut(s) 108, 286, 1633, 1669, 1899, 2875, 2905, 2929, 3061
MfeI CAATTG 3 cut(s) 975, 2292, 3066
MflI RGATCY 5 cut(s) 286, 1669, 2875, 2929, 3061
MhlI GDGCHC 5 cut(s) 42, 736, 906, 1388, 3480
MlsI TGGCCA 1 cut(s) 3350
MluNI TGGCCA 1 cut(s) 3350
MlyI GAGTC 3 cut(s) 1996, 3268, 3290
MmeI TCCRAC 6 cut(s) 1012, 1202, 1887, 2974, 3076, 3237
Mox20I TGGCCA 1 cut(s) 3350
Mph1103I ATGCAT 3 cut(s) 1079, 1626, 2560
MroI TCCGGA 2 cut(s) 447, 1685
MroXI GAANNNNTTC 3 cut(s) 969, 1323, 1743
MscI TGGCCA 1 cut(s) 3350
MslI CAYNNNNRTG 1 cut(s) 1072
Msp20I TGGCCA 1 cut(s) 3350
MspA1I CMGCKG 3 cut(s) 20, 565, 1888
MspI CCGG 8 cut(s) 167, 343, 448, 1018, 1686, 1882, 3034, 3354
MspR9I CCNGG 7 cut(s) 168, 498, 1019, 1123, 1300, 2850, 3035
MunI CAATTG 3 cut(s) 975, 2292, 3066
Mva1269I GAATGC 4 cut(s) 543, 597, 1964, 2821
MvaI CCWGG 4 cut(s) 498, 1123, 1300, 2850
MvnI CGCG 1 cut(s) 3432
MwoI GCNNNNNNNGC 3 cut(s) 161, 2564, 3225
NciI CCSGG 3 cut(s) 168, 1019, 3035
NdeI CATATG 1 cut(s) 2218
NdeII GATC 9 cut(s) 108, 286, 1633, 1669, 1899, 2875, 2905, 2929, 3061
NheI GCTAGC 2 cut(s) 1825, 3515
NlaIV GGNNCC 7 cut(s) 347, 362, 818, 1663, 2375, 3032, 3063
NmuCI GTSAC 6 cut(s) 475, 1271, 1294, 1344, 3404, 3494
NsiI ATGCAT 3 cut(s) 1079, 1626, 2560
NspI RCATGY 5 cut(s) 646, 1077, 1312, 1583, 2016
NspV TTCGAA 1 cut(s) 2698
PaeI GCATGC 1 cut(s) 1077
PaeR7I CTCGAG 1 cut(s) 1040
PaqCI CACCTGC 1 cut(s) 79
PasI CCCWGGG 1 cut(s) 497
PceI AGGCCT 1 cut(s) 2091
PciI ACATGT 2 cut(s) 642, 2012
PctI GAATGC 4 cut(s) 543, 597, 1964, 2821
PdmI GAANNNNTTC 3 cut(s) 969, 1323, 1743
PflFI GACNNNGTC 2 cut(s) 1151, 3280
PflMI CCANNNNNTGG 1 cut(s) 2219
PfoI TCCNGGA 1 cut(s) 166
PinAI ACCGGT 1 cut(s) 342
PleI GAGTC 3 cut(s) 1995, 3267, 3289
PmaCI CACGTG 2 cut(s) 2128, 2810
PmlI CACGTG 2 cut(s) 2128, 2810
PpsI GAGTC 3 cut(s) 1995, 3267, 3289
Ppu21I YACGTR 3 cut(s) 1292, 2128, 2810
PscI ACATGT 2 cut(s) 642, 2012
Psp124BI GAGCTC 2 cut(s) 42, 1388
Psp6I CCWGG 4 cut(s) 496, 1121, 1298, 2848
PspCI CACGTG 2 cut(s) 2128, 2810
PspFI CCCAGC 2 cut(s) 2212, 3466
PspGI CCWGG 4 cut(s) 496, 1121, 1298, 2848
PspN4I GGNNCC 7 cut(s) 347, 362, 818, 1663, 2375, 3032, 3063
PstI CTGCAG 1 cut(s) 564
PstNI CAGNNNCTG 1 cut(s) 2024
PsuI RGATCY 5 cut(s) 286, 1669, 2875, 2929, 3061
PsyI GACNNNGTC 2 cut(s) 1151, 3280
RsaI GTAC 9 cut(s) 347, 368, 389, 620, 988, 1290, 2017, 2945, 3124
RsaNI GTAC 9 cut(s) 346, 367, 388, 619, 987, 1289, 2016, 2944, 3123
RseI CAYNNNNRTG 1 cut(s) 1072
SacI GAGCTC 2 cut(s) 42, 1388
Sau3AI GATC 9 cut(s) 108, 286, 1633, 1669, 1899, 2875, 2905, 2929, 3061
ScaI AGTACT 3 cut(s) 389, 2945, 3124
SchI GAGTC 3 cut(s) 1996, 3268, 3290
ScrFI CCNGG 7 cut(s) 168, 498, 1019, 1123, 1300, 2850, 3035
SduI GDGCHC 5 cut(s) 42, 736, 906, 1388, 3480
SfaNI GCATC 7 cut(s) 85, 735, 1417, 1887, 2567, 2710, 2977
SfcI CTRYAG 3 cut(s) 252, 560, 1761
Sfr274I CTCGAG 1 cut(s) 1040
SfuI TTCGAA 1 cut(s) 2698
SinI GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
SlaI CTCGAG 1 cut(s) 1040
SmiMI CAYNNNNRTG 1 cut(s) 1072
SmlI CTYRAG 2 cut(s) 143, 1040
SmoI CTYRAG 2 cut(s) 143, 1040
SphI GCATGC 1 cut(s) 1077
SseBI AGGCCT 1 cut(s) 2091
SspI AATATT 2 cut(s) 65, 852
SspMI CTAG 9 cut(s) 59, 996, 1775, 1826, 2537, 2933, 3482, 3510, 3516
SstI GAGCTC 2 cut(s) 42, 1388
StuI AGGCCT 1 cut(s) 2091
StyD4I CCNGG 7 cut(s) 166, 496, 1017, 1121, 1298, 2848, 3033
StyI CCWWGG 3 cut(s) 306, 3317, 3481
TaaI ACNGT 4 cut(s) 1288, 1525, 1765, 3413
TaiI ACGT 6 cut(s) 1294, 2110, 2130, 2812, 3459, 3501
TaqI TCGA 9 cut(s) 107, 123, 1041, 1607, 1718, 2698, 2904, 3148, 3294
TaqII GACCGA 1 cut(s) 1290
TatI WGTACW 6 cut(s) 387, 618, 986, 2015, 2943, 3122
TauI GCSGC 5 cut(s) 243, 741, 1888, 3109, 3432
TscAI CASTG 8 cut(s) 1074, 1368, 1386, 1770, 2029, 2365, 2635, 3403
TseFI GTSAC 6 cut(s) 475, 1271, 1294, 1344, 3404, 3494
TseI GCWGC 5 cut(s) 116, 562, 1045, 2252, 3141
Tsp45I GTSAC 6 cut(s) 475, 1271, 1294, 1344, 3404, 3494
TspGWI ACGGA 2 cut(s) 3293, 3384
TspRI CASTG 8 cut(s) 1074, 1368, 1386, 1770, 2029, 2365, 2635, 3403
Tth111I GACNNNGTC 2 cut(s) 1151, 3280
Van91I CCANNNNNTGG 1 cut(s) 2219
VpaK11BI GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
XbaI TCTAGA 2 cut(s) 995, 2932
XceI RCATGY 5 cut(s) 646, 1077, 1312, 1583, 2016
XcmI CCANNNNNNNNNTGG 1 cut(s) 2653
XhoI CTCGAG 1 cut(s) 1040
XmaJI CCTAGG 1 cut(s) 3481
XmiI GTMKAC 2 cut(s) 90, 608
XmnI GAANNNNTTC 3 cut(s) 969, 1323, 1743
XspI CTAG 9 cut(s) 59, 996, 1775, 1826, 2537, 2933, 3482, 3510, 3516
ZrmI AGTACT 3 cut(s) 389, 2945, 3124
Zsp2I ATGCAT 3 cut(s) 1079, 1626, 2560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.