Rmu_sc0006932.1_g000005

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006932.1
OG0016266
Physical Location & Seq
Reverse (-)
13846 .. 14298
453 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 453 bp
atggacgaaaatcttatcgaggaaacatgcagaaagacaccagactacgatatatgtctcttttgtcttaaatcgaccggtggtagtgggatcttgactgcaaatcgcacacgcttggccgacgtaatgattgatgtagtccagcagagatcaatcactactatgaacaatatgagtgaccttcttggtaaaacacctggagatacatatgaaccgttatctacatgtattcaaaattacattgcgatcgtaactcgttatatcccggcggccaagaagttaatttacacaagtgagcttaagatggcaaagaaaataatggataacattccaaatgttgcaaaatcatgtgaggattcattcagaggcaagctgactcctattgctgacttgcatataggtgttcaaaaggtttcagctattgctatagcaataatagggcaattgggttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

150

Amino Acids

16.46

Weight (kDa)

7.58

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 4 - 145 3e-12 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 269
AclWI GGATC 1 cut(s) 98
AcoI YGGCCR 2 cut(s) 117, 270
AflII CTTAAG 1 cut(s) 299
AflIII ACRYGT 1 cut(s) 224
AgeI ACCGGT 1 cut(s) 77
AgsI TTSAA 2 cut(s) 233, 407
AjnI CCWGG 1 cut(s) 196
AluBI AGCT 3 cut(s) 298, 373, 419
AluI AGCT 3 cut(s) 298, 373, 419
Alw26I GTCTC 1 cut(s) 62
AlwI GGATC 1 cut(s) 98
AoxI GGCC 2 cut(s) 117, 270
AsiGI ACCGGT 1 cut(s) 77
AsuC2I CCSGG 1 cut(s) 266
BarI GAAGNNNNNNTAC 2 cut(s) 269, 301
BccI CCATC 1 cut(s) 298
BciT130I CCWGG 1 cut(s) 198
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 1 cut(s) 62
BfmI CTRYAG 1 cut(s) 426
BfrI CTTAAG 1 cut(s) 299
BisI GCNGC 1 cut(s) 270
BlsI GCNGC 1 cut(s) 271
Bme1390I CCNGG 2 cut(s) 198, 266
BmrFI CCNGG 2 cut(s) 198, 266
BpmI CTGGAG 1 cut(s) 219
BpuMI CCSGG 1 cut(s) 266
BsaWI WCCGGW 1 cut(s) 77
Bse118I RCCGGY 1 cut(s) 77
Bse3DI GCAATG 1 cut(s) 240
BseBI CCWGG 1 cut(s) 198
BseMI GCAATG 1 cut(s) 240
Bsh1285I CGRYCG 2 cut(s) 78, 249
BshFI GGCC 2 cut(s) 119, 272
BshTI ACCGGT 1 cut(s) 77
BsiEI CGRYCG 2 cut(s) 78, 249
BsiSI CCGG 2 cut(s) 78, 266
BsmAI GTCTC 1 cut(s) 62
BsnI GGCC 2 cut(s) 119, 272
Bsp143I GATC 3 cut(s) 90, 149, 246
BspACI CCGC 1 cut(s) 269
BspANI GGCC 2 cut(s) 119, 272
BspPI GGATC 1 cut(s) 98
BspTI CTTAAG 1 cut(s) 299
BsrDI GCAATG 1 cut(s) 240
BsrFI RCCGGY 1 cut(s) 77
BssAI RCCGGY 1 cut(s) 77
BssMI GATC 3 cut(s) 90, 149, 246
Bst2UI CCWGG 1 cut(s) 198
Bst4CI ACNGT 1 cut(s) 216
BstAFI CTTAAG 1 cut(s) 299
BstC8I GCNNGC 1 cut(s) 371
BstKTI GATC 3 cut(s) 93, 152, 249
BstMAI GTCTC 1 cut(s) 62
BstMBI GATC 3 cut(s) 90, 149, 246
BstMCI CGRYCG 2 cut(s) 78, 249
BstNI CCWGG 1 cut(s) 198
BstNSI RCATGY 2 cut(s) 30, 228
BstSCI CCNGG 2 cut(s) 196, 264
BstSFI CTRYAG 1 cut(s) 426
BstX2I RGATCY 1 cut(s) 90
BstYI RGATCY 1 cut(s) 90
BsuRI GGCC 2 cut(s) 119, 272
Cac8I GCNNGC 1 cut(s) 371
Cfr10I RCCGGY 1 cut(s) 77
CspAI ACCGGT 1 cut(s) 77
CviAII CATG 3 cut(s) 27, 225, 348
CviJI RGCY 5 cut(s) 119, 272, 298, 373, 419
CviKI_1 RGCY 5 cut(s) 119, 272, 298, 373, 419
DpnI GATC 3 cut(s) 92, 151, 248
DpnII GATC 3 cut(s) 90, 149, 246
EaeI YGGCCR 2 cut(s) 117, 270
EcoRII CCWGG 1 cut(s) 196
FaeI CATG 3 cut(s) 30, 228, 351
FatI CATG 3 cut(s) 26, 224, 347
FauNDI CATATG 1 cut(s) 208
Fnu4HI GCNGC 1 cut(s) 270
Fsp4HI GCNGC 1 cut(s) 270
GluI GCNGC 1 cut(s) 270
GsuI CTGGAG 1 cut(s) 219
HaeIII GGCC 2 cut(s) 119, 272
HapII CCGG 2 cut(s) 78, 266
Hin1II CATG 3 cut(s) 30, 228, 351
HinfI GANTC 2 cut(s) 356, 376
HpaII CCGG 2 cut(s) 78, 266
Hpy188I TCNGA 1 cut(s) 365
Hpy188III TCNNGA 1 cut(s) 94
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 1 cut(s) 191
HpyCH4III ACNGT 1 cut(s) 216
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 4 cut(s) 30, 101, 341, 394
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 3 cut(s) 30, 228, 351
Kzo9I GATC 3 cut(s) 90, 149, 246
LpnPI CCDG 6 cut(s) 54, 91, 155, 183, 210, 279
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 2 cut(s) 176, 250
MalI GATC 3 cut(s) 92, 151, 248
MboI GATC 3 cut(s) 90, 149, 246
MfeI CAATTG 1 cut(s) 443
MflI RGATCY 1 cut(s) 90
MluCI AATT 3 cut(s) 235, 282, 443
MlyI GAGTC 1 cut(s) 370
MnlI CCTC 3 cut(s) 13, 346, 359
MseI TTAA 3 cut(s) 69, 281, 300
MslI CAYNNNNRTG 2 cut(s) 161, 399
MspCI CTTAAG 1 cut(s) 299
MspI CCGG 2 cut(s) 78, 266
MspR9I CCNGG 2 cut(s) 198, 266
MunI CAATTG 1 cut(s) 443
MvaI CCWGG 1 cut(s) 198
NciI CCSGG 1 cut(s) 266
NdeI CATATG 1 cut(s) 208
NdeII GATC 3 cut(s) 90, 149, 246
NlaIII CATG 3 cut(s) 30, 228, 351
NmuCI GTSAC 1 cut(s) 176
NspI RCATGY 2 cut(s) 30, 228
PciI ACATGT 1 cut(s) 224
PfeI GAWTC 1 cut(s) 356
PinAI ACCGGT 1 cut(s) 77
PkrI GCNGC 1 cut(s) 271
Ple19I CGATCG 1 cut(s) 249
PleI GAGTC 1 cut(s) 370
PpsI GAGTC 1 cut(s) 370
PscI ACATGT 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 196
PspGI CCWGG 1 cut(s) 196
PsuI RGATCY 1 cut(s) 90
PvuI CGATCG 1 cut(s) 249
RseI CAYNNNNRTG 2 cut(s) 161, 399
SaqAI TTAA 3 cut(s) 69, 281, 300
SatI GCNGC 1 cut(s) 270
Sau3AI GATC 3 cut(s) 90, 149, 246
SchI GAGTC 1 cut(s) 370
ScrFI CCNGG 2 cut(s) 198, 266
SetI ASST 8 cut(s) 126, 183, 199, 300, 375, 403, 414, 421
SfcI CTRYAG 1 cut(s) 426
SmiMI CAYNNNNRTG 2 cut(s) 161, 399
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
Sse9I AATT 3 cut(s) 235, 282, 443
SsiI CCGC 1 cut(s) 269
StyD4I CCNGG 2 cut(s) 196, 264
TaaI ACNGT 1 cut(s) 216
TaiI ACGT 1 cut(s) 126
TaqI TCGA 2 cut(s) 18, 74
TasI AATT 3 cut(s) 235, 282, 443
TauI GCSGC 1 cut(s) 272
TfiI GAWTC 1 cut(s) 356
Tru1I TTAA 3 cut(s) 69, 281, 300
Tru9I TTAA 3 cut(s) 69, 281, 300
TseFI GTSAC 1 cut(s) 176
Tsp45I GTSAC 1 cut(s) 176
TspDTI ATGAA 3 cut(s) 179, 225, 348
Vha464I CTTAAG 1 cut(s) 299
XceI RCATGY 2 cut(s) 30, 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.