Rh7DG361000

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0016266
Physical Location & Seq
Reverse (-)
48045648 .. 48046584
937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG361000.1

Sequence Viewer

Length: 546 bp
ATGATGAATCCATCAACCCCCATATTTCTCATTCAACTTATTTGTTTTCTCATATTCCTTCCAATAAGCCAATGCAGCCAAGTTTTCTTTCCAATGGACGAAAATCTTATCGAGGAAACATGCAGAAAGACACCAGACTACGATATATGTCTCTTTTGTCTTAAATCGACCGGTGGTAGTGGGATCTTGACTGCAAATCGCACACGCTTGGCCGACGTAATGATTGATGTAGTCCAGCAGAGATCAATCACTACTATGAACAATATGAGTGACCTTCTTGGTAAAACACCTGGAGATACATATCAACCGTTATCTACATGTATTCAAAATTACATTGCGATCGTAACTCGTTATATCCCGGCGGCCAAGAAGTTAATTTACACGAGTGAGCTTAAGATGGCAAAGAAAATAATGGATAACATTCCAAATGTTGCAAAATCATGTGAGGATTCATTCAGAGGCAAGCTGACTCCTATTGCTGACTTGAATATAGGAGTTCAAAAGGTTTCAGCTATTGCTATAGCAATAATAGGGCAATTGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

181

Amino Acids

20.01

Weight (kDa)

8.14

Isoelectric Point (pI)

34.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 35 - 176 1.1e-12 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 362
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 2 cut(s) 210, 363
AflII CTTAAG 1 cut(s) 392
AflIII ACRYGT 1 cut(s) 317
AgeI ACCGGT 1 cut(s) 170
AgsI TTSAA 4 cut(s) 35, 326, 487, 500
AjnI CCWGG 1 cut(s) 289
AjuI GAANNNNNNNTTGG 2 cut(s) 72, 104
AluBI AGCT 3 cut(s) 391, 466, 512
AluI AGCT 3 cut(s) 391, 466, 512
Alw26I GTCTC 1 cut(s) 155
AlwI GGATC 1 cut(s) 191
AoxI GGCC 2 cut(s) 210, 363
ApeKI GCWGC 1 cut(s) 75
AsiGI ACCGGT 1 cut(s) 170
AsuC2I CCSGG 1 cut(s) 359
BarI GAAGNNNNNNTAC 2 cut(s) 362, 394
BauI CACGAG 1 cut(s) 382
BbvI GCAGC 1 cut(s) 87
BccI CCATC 2 cut(s) 19, 391
BciT130I CCWGG 1 cut(s) 291
BcnI CCSGG 1 cut(s) 359
BcoDI GTCTC 1 cut(s) 155
BfmI CTRYAG 1 cut(s) 519
BfrI CTTAAG 1 cut(s) 392
BisI GCNGC 2 cut(s) 76, 363
BlsI GCNGC 2 cut(s) 77, 364
Bme1390I CCNGG 2 cut(s) 291, 359
BmrFI CCNGG 2 cut(s) 291, 359
BpmI CTGGAG 1 cut(s) 312
BpuMI CCSGG 1 cut(s) 359
BsaBI GATNNNNATC 1 cut(s) 300
BsaWI WCCGGW 1 cut(s) 170
Bse118I RCCGGY 1 cut(s) 170
Bse3DI GCAATG 1 cut(s) 333
Bse8I GATNNNNATC 1 cut(s) 300
BseBI CCWGG 1 cut(s) 291
BseJI GATNNNNATC 1 cut(s) 300
BseMI GCAATG 1 cut(s) 333
BseXI GCAGC 1 cut(s) 87
Bsh1285I CGRYCG 2 cut(s) 171, 342
BshFI GGCC 2 cut(s) 212, 365
BshTI ACCGGT 1 cut(s) 170
BsiEI CGRYCG 2 cut(s) 171, 342
BsiSI CCGG 2 cut(s) 171, 359
BsmAI GTCTC 1 cut(s) 155
BsnI GGCC 2 cut(s) 212, 365
Bsp143I GATC 3 cut(s) 183, 242, 339
BspACI CCGC 1 cut(s) 362
BspANI GGCC 2 cut(s) 212, 365
BspPI GGATC 1 cut(s) 191
BspTI CTTAAG 1 cut(s) 392
BsrDI GCAATG 1 cut(s) 333
BsrFI RCCGGY 1 cut(s) 170
BssAI RCCGGY 1 cut(s) 170
BssMI GATC 3 cut(s) 183, 242, 339
BssSI CACGAG 1 cut(s) 382
Bst2BI CACGAG 1 cut(s) 382
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 1 cut(s) 309
BstAFI CTTAAG 1 cut(s) 392
BstC8I GCNNGC 1 cut(s) 464
BstKTI GATC 3 cut(s) 186, 245, 342
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 3 cut(s) 183, 242, 339
BstMCI CGRYCG 2 cut(s) 171, 342
BstMWI GCNNNNNNNGC 1 cut(s) 75
BstNI CCWGG 1 cut(s) 291
BstNSI RCATGY 2 cut(s) 123, 321
BstSCI CCNGG 2 cut(s) 289, 357
BstSFI CTRYAG 1 cut(s) 519
BstV1I GCAGC 1 cut(s) 87
BstX2I RGATCY 1 cut(s) 183
BstYI RGATCY 1 cut(s) 183
BsuRI GGCC 2 cut(s) 212, 365
Cac8I GCNNGC 1 cut(s) 464
Cfr10I RCCGGY 1 cut(s) 170
CspAI ACCGGT 1 cut(s) 170
CviAII CATG 3 cut(s) 120, 318, 441
CviJI RGCY 7 cut(s) 69, 78, 212, 365, 391, 466, 512
CviKI_1 RGCY 7 cut(s) 69, 78, 212, 365, 391, 466, 512
DpnI GATC 3 cut(s) 185, 244, 341
DpnII GATC 3 cut(s) 183, 242, 339
EaeI YGGCCR 2 cut(s) 210, 363
EcoRII CCWGG 1 cut(s) 289
FaeI CATG 3 cut(s) 123, 321, 444
FatI CATG 3 cut(s) 119, 317, 440
Fnu4HI GCNGC 2 cut(s) 76, 363
Fsp4HI GCNGC 2 cut(s) 76, 363
GluI GCNGC 2 cut(s) 76, 363
GsuI CTGGAG 1 cut(s) 312
HaeIII GGCC 2 cut(s) 212, 365
HapII CCGG 2 cut(s) 171, 359
Hin1II CATG 3 cut(s) 123, 321, 444
HinfI GANTC 3 cut(s) 7, 449, 469
HpaII CCGG 2 cut(s) 171, 359
Hpy188I TCNGA 1 cut(s) 458
Hpy188III TCNNGA 1 cut(s) 187
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 2 cut(s) 68, 284
HpyCH4III ACNGT 1 cut(s) 309
HpyCH4IV ACGT 1 cut(s) 216
HpyCH4V TGCA 4 cut(s) 75, 123, 194, 434
HpyF10VI GCNNNNNNNGC 1 cut(s) 75
HpySE526I ACGT 1 cut(s) 216
Hsp92II CATG 3 cut(s) 123, 321, 444
Kzo9I GATC 3 cut(s) 183, 242, 339
LpnPI CCDG 6 cut(s) 147, 184, 248, 276, 303, 372
Lsp1109I GCAGC 1 cut(s) 87
MaeII ACGT 1 cut(s) 216
MaeIII GTNAC 2 cut(s) 269, 343
MalI GATC 3 cut(s) 185, 244, 341
MboI GATC 3 cut(s) 183, 242, 339
MfeI CAATTG 1 cut(s) 536
MflI RGATCY 1 cut(s) 183
MluCI AATT 3 cut(s) 328, 375, 536
MlyI GAGTC 1 cut(s) 463
MnlI CCTC 3 cut(s) 106, 439, 452
MseI TTAA 3 cut(s) 162, 374, 393
MslI CAYNNNNRTG 1 cut(s) 254
MspCI CTTAAG 1 cut(s) 392
MspI CCGG 2 cut(s) 171, 359
MspR9I CCNGG 2 cut(s) 291, 359
MunI CAATTG 1 cut(s) 536
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 1 cut(s) 75
NciI CCSGG 1 cut(s) 359
NdeII GATC 3 cut(s) 183, 242, 339
NlaIII CATG 3 cut(s) 123, 321, 444
NmuCI GTSAC 1 cut(s) 269
NspI RCATGY 2 cut(s) 123, 321
PciI ACATGT 1 cut(s) 317
PfeI GAWTC 2 cut(s) 7, 449
PinAI ACCGGT 1 cut(s) 170
PkrI GCNGC 2 cut(s) 77, 364
Ple19I CGATCG 1 cut(s) 342
PleI GAGTC 1 cut(s) 463
PpsI GAGTC 1 cut(s) 463
PscI ACATGT 1 cut(s) 317
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PsuI RGATCY 1 cut(s) 183
PvuI CGATCG 1 cut(s) 342
RseI CAYNNNNRTG 1 cut(s) 254
SaqAI TTAA 3 cut(s) 162, 374, 393
SatI GCNGC 2 cut(s) 76, 363
Sau3AI GATC 3 cut(s) 183, 242, 339
SchI GAGTC 1 cut(s) 463
ScrFI CCNGG 2 cut(s) 291, 359
SetI ASST 7 cut(s) 219, 276, 292, 393, 468, 507, 514
SfcI CTRYAG 1 cut(s) 519
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
Sse9I AATT 3 cut(s) 328, 375, 536
SsiI CCGC 1 cut(s) 362
StyD4I CCNGG 2 cut(s) 289, 357
TaaI ACNGT 1 cut(s) 309
TaiI ACGT 1 cut(s) 219
TaqI TCGA 2 cut(s) 111, 167
TasI AATT 3 cut(s) 328, 375, 536
TauI GCSGC 1 cut(s) 365
TfiI GAWTC 2 cut(s) 7, 449
Tru1I TTAA 3 cut(s) 162, 374, 393
Tru9I TTAA 3 cut(s) 162, 374, 393
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 1 cut(s) 75
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 3 cut(s) 20, 272, 441
Vha464I CTTAAG 1 cut(s) 392
XceI RCATGY 2 cut(s) 123, 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.