Rroxscaffold_3G00234930

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0016266
Physical Location & Seq
Forward (+)
21616851 .. 21619166
2316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00234930.1

Sequence Viewer

Length: 513 bp
ATGAGTGGTGGTGCCGGTTTGGCTCCGGTTCGACCGATCTTGGGAGATGTTGTGAGGTCAATGGCCGGAGATCCTCCTCCAGTCGGAGAAACATGCAGAAAGACACCAGACTACGATCTATGTCTCTTTTATTTTAAATCGACCAGTGATAGTGGGATCTTGACTGCAAATCGCACACGCTTGGCCGACGTAATGATTGATGTAGTCCGGCATATATCAACCAATACTTGGAAAAAAATTGATGAGCTTCTTGGTAAAACACCTGGAGATAAACCGTTAACTACATGTCTTGTAAATTACTATACGATCGTAAGCCATTATATCCCGGCGGCCAAGAAGACAATTTATACGAGTAATCGTAAGGTAGCAGGGAAACTAATTGGTAACATTCCAAATGTTGCAAAATCATGTGAGGATTCATTCAGAGGCAATCCGTCTCCTCTTACTGACTTGAATATATTTGTCCAAAAGGTTGCAGCTATTACTGCAGCAATAATAGGGCAATTGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

170

Amino Acids

18.28

Weight (kDa)

9.21

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 30 - 164 8.2e-09 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 11
AccB7I CCANNNNNTGG 1 cut(s) 228
AciI CCGC 1 cut(s) 329
AclWI GGATC 2 cut(s) 65, 164
AcoI YGGCCR 3 cut(s) 63, 183, 330
AfiI CCNNNNNNNGG 3 cut(s) 41, 83, 228
AflIII ACRYGT 1 cut(s) 284
AgsI TTSAA 1 cut(s) 454
AjnI CCWGG 1 cut(s) 262
AluBI AGCT 2 cut(s) 247, 479
AluI AGCT 2 cut(s) 247, 479
Alw26I GTCTC 2 cut(s) 128, 441
AlwI GGATC 2 cut(s) 65, 164
AoxI GGCC 3 cut(s) 63, 183, 330
ApeKI GCWGC 2 cut(s) 476, 488
AsuC2I CCSGG 1 cut(s) 326
BanI GGYRCC 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 344
BbvI GCAGC 2 cut(s) 488, 500
BciT130I CCWGG 1 cut(s) 264
BcnI CCSGG 1 cut(s) 326
BcoDI GTCTC 2 cut(s) 128, 441
BfmI CTRYAG 1 cut(s) 486
BglI GCCNNNNNGGC 1 cut(s) 20
BisI GCNGC 3 cut(s) 330, 477, 489
BlsI GCNGC 3 cut(s) 331, 478, 490
Bme1390I CCNGG 2 cut(s) 264, 326
BmiI GGNNCC 2 cut(s) 13, 24
BmrFI CCNGG 2 cut(s) 264, 326
BpiI GAAGAC 1 cut(s) 344
BpmI CTGGAG 2 cut(s) 63, 285
BpuMI CCSGG 1 cut(s) 326
BsaWI WCCGGW 1 cut(s) 25
BsaXI ACNNNNNCTCC 2 cut(s) 36, 66
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 83, 228
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 2 cut(s) 80, 144
BseBI CCWGG 1 cut(s) 264
BseLI CCNNNNNNNGG 3 cut(s) 41, 83, 228
BseNI ACTGG 2 cut(s) 80, 144
BseRI GAGGAG 2 cut(s) 66, 429
BseXI GCAGC 2 cut(s) 488, 500
Bsh1285I CGRYCG 2 cut(s) 35, 309
BshFI GGCC 3 cut(s) 65, 185, 332
BshNI GGYRCC 1 cut(s) 11
BsiEI CGRYCG 2 cut(s) 35, 309
BsiSI CCGG 5 cut(s) 15, 26, 66, 208, 326
BslI CCNNNNNNNGG 3 cut(s) 41, 83, 228
BsmAI GTCTC 2 cut(s) 128, 441
BsmBI CGTCTC 1 cut(s) 441
BsnI GGCC 3 cut(s) 65, 185, 332
Bsp143I GATC 5 cut(s) 36, 70, 115, 156, 306
BspACI CCGC 1 cut(s) 329
BspANI GGCC 3 cut(s) 65, 185, 332
BspLI GGNNCC 2 cut(s) 13, 24
BspMAI CTGCAG 1 cut(s) 490
BspPI GGATC 2 cut(s) 65, 164
BspT107I GGYRCC 1 cut(s) 11
BsrFI RCCGGY 1 cut(s) 14
BsrI ACTGG 2 cut(s) 80, 144
BssAI RCCGGY 1 cut(s) 14
BssMI GATC 5 cut(s) 36, 70, 115, 156, 306
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 1 cut(s) 276
BstKTI GATC 5 cut(s) 39, 73, 118, 159, 309
BstMAI GTCTC 2 cut(s) 128, 441
BstMBI GATC 5 cut(s) 36, 70, 115, 156, 306
BstMCI CGRYCG 2 cut(s) 35, 309
BstMWI GCNNNNNNNGC 2 cut(s) 20, 485
BstNI CCWGG 1 cut(s) 264
BstNSI RCATGY 2 cut(s) 96, 288
BstSCI CCNGG 2 cut(s) 262, 324
BstSFI CTRYAG 1 cut(s) 486
BstV1I GCAGC 2 cut(s) 488, 500
BstV2I GAAGAC 1 cut(s) 344
BstX2I RGATCY 2 cut(s) 70, 156
BstYI RGATCY 2 cut(s) 70, 156
BsuRI GGCC 3 cut(s) 65, 185, 332
BtsIMutI CAGTG 1 cut(s) 151
Cfr10I RCCGGY 1 cut(s) 14
CviAII CATG 3 cut(s) 93, 285, 408
CviJI RGCY 7 cut(s) 23, 65, 185, 247, 315, 332, 479
CviKI_1 RGCY 7 cut(s) 23, 65, 185, 247, 315, 332, 479
DpnI GATC 5 cut(s) 38, 72, 117, 158, 308
DpnII GATC 5 cut(s) 36, 70, 115, 156, 306
DraI TTTAAA 1 cut(s) 136
EaeI YGGCCR 3 cut(s) 63, 183, 330
EcoRII CCWGG 1 cut(s) 262
Esp3I CGTCTC 1 cut(s) 441
FaeI CATG 3 cut(s) 96, 288, 411
FatI CATG 3 cut(s) 92, 284, 407
Fnu4HI GCNGC 3 cut(s) 330, 477, 489
Fsp4HI GCNGC 3 cut(s) 330, 477, 489
GluI GCNGC 3 cut(s) 330, 477, 489
GsuI CTGGAG 2 cut(s) 63, 285
HaeIII GGCC 3 cut(s) 65, 185, 332
HapII CCGG 5 cut(s) 15, 26, 66, 208, 326
Hin1II CATG 3 cut(s) 96, 288, 411
HincII GTYRAC 1 cut(s) 279
HindII GTYRAC 1 cut(s) 279
HinfI GANTC 1 cut(s) 416
HpaI GTTAAC 1 cut(s) 279
HpaII CCGG 5 cut(s) 15, 26, 66, 208, 326
Hpy166II GTNNAC 1 cut(s) 279
Hpy188I TCNGA 2 cut(s) 86, 425
Hpy188III TCNNGA 1 cut(s) 160
Hpy8I GTNNAC 1 cut(s) 279
Hpy99I CGWCG 1 cut(s) 191
HpyCH4III ACNGT 1 cut(s) 276
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 5 cut(s) 96, 167, 401, 476, 488
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 485
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 3 cut(s) 96, 288, 411
KspAI GTTAAC 1 cut(s) 279
Kzo9I GATC 5 cut(s) 36, 70, 115, 156, 306
LmnI GCTCC 1 cut(s) 28
Lsp1109I GCAGC 2 cut(s) 488, 500
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 1 cut(s) 383
MalI GATC 5 cut(s) 38, 72, 117, 158, 308
MboI GATC 5 cut(s) 36, 70, 115, 156, 306
MboII GAAGA 1 cut(s) 349
MfeI CAATTG 1 cut(s) 503
MflI RGATCY 2 cut(s) 70, 156
MluCI AATT 5 cut(s) 237, 295, 342, 378, 503
MmeI TCCRAC 1 cut(s) 64
MnlI CCTC 6 cut(s) 48, 84, 87, 406, 419, 450
MseI TTAA 2 cut(s) 135, 278
MspI CCGG 5 cut(s) 15, 26, 66, 208, 326
MspR9I CCNGG 2 cut(s) 264, 326
MunI CAATTG 1 cut(s) 503
MvaI CCWGG 1 cut(s) 264
MwoI GCNNNNNNNGC 2 cut(s) 20, 485
NciI CCSGG 1 cut(s) 326
NdeII GATC 5 cut(s) 36, 70, 115, 156, 306
NlaIII CATG 3 cut(s) 96, 288, 411
NlaIV GGNNCC 2 cut(s) 13, 24
NspI RCATGY 2 cut(s) 96, 288
PciI ACATGT 1 cut(s) 284
PfeI GAWTC 1 cut(s) 416
PflMI CCANNNNNTGG 1 cut(s) 228
PkrI GCNGC 3 cut(s) 331, 478, 490
Ple19I CGATCG 1 cut(s) 309
PscI ACATGT 1 cut(s) 284
Psp6I CCWGG 1 cut(s) 262
PspGI CCWGG 1 cut(s) 262
PspN4I GGNNCC 2 cut(s) 13, 24
PstI CTGCAG 1 cut(s) 490
PsuI RGATCY 2 cut(s) 70, 156
PvuI CGATCG 1 cut(s) 309
SaqAI TTAA 2 cut(s) 135, 278
SatI GCNGC 3 cut(s) 330, 477, 489
Sau3AI GATC 5 cut(s) 36, 70, 115, 156, 306
ScrFI CCNGG 2 cut(s) 264, 326
SetI ASST 7 cut(s) 59, 192, 249, 265, 366, 474, 481
SfcI CTRYAG 1 cut(s) 486
Sse9I AATT 5 cut(s) 237, 295, 342, 378, 503
SsiI CCGC 1 cut(s) 329
StyD4I CCNGG 2 cut(s) 262, 324
TaaI ACNGT 1 cut(s) 276
TaiI ACGT 1 cut(s) 192
TaqI TCGA 2 cut(s) 31, 140
TaqII GACCGA 1 cut(s) 49
TasI AATT 5 cut(s) 237, 295, 342, 378, 503
TauI GCSGC 1 cut(s) 332
TfiI GAWTC 1 cut(s) 416
Tru1I TTAA 2 cut(s) 135, 278
Tru9I TTAA 2 cut(s) 135, 278
TscAI CASTG 1 cut(s) 151
TseI GCWGC 2 cut(s) 476, 488
TspDTI ATGAA 1 cut(s) 408
TspGWI ACGGA 1 cut(s) 423
TspRI CASTG 1 cut(s) 151
Van91I CCANNNNNTGG 1 cut(s) 228
XceI RCATGY 2 cut(s) 96, 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.