Rh7DG361100

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0016266
Physical Location & Seq
Reverse (-)
48050291 .. 48050737
447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG361100.1

Sequence Viewer

Length: 447 bp
ATGGACCAAAATCTTATTGAGGAAACATGCAGAAAGACACCAGACTATGATCTATGTATCTTTTATTTTAAATCGACCAGTGATAGTGGGATCTTGACTGCAAATCGCACACGCTTGGCCGACGTAATGATTGATGTAGTCCGGCAGATATCAACCAATACTCGGAAAAAAATTGATGAGCTTCTTGGTAAAACACCTGGAGATAAACCGTTAACTACATGTCTTGTATATTACTATACGATCGTAAGCCATTATATCCCGGCGGCCAAGAAGACAATTTATACGAGTGATCGTAAGGTGGCAGGGAGACTAATTGGTCACATTCCAAATGTTGCAAAATCATGTGAGGATTCATTCAGAGGCAATCCGTCTCCTCTTACTGACTTGAATATATTTGTCCAAAAGGTTGCAGCTATTACTGCAGCAATAATAGGGCAATTGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

148

Amino Acids

16.43

Weight (kDa)

8.88

Isoelectric Point (pI)

32.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 4 - 142 1.3e-10 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 315
AciI CCGC 1 cut(s) 263
AclWI GGATC 1 cut(s) 98
AcoI YGGCCR 2 cut(s) 117, 264
AfiI CCNNNNNNNGG 1 cut(s) 162
AflIII ACRYGT 1 cut(s) 218
AgsI TTSAA 1 cut(s) 388
AjnI CCWGG 1 cut(s) 196
AluBI AGCT 2 cut(s) 181, 413
AluI AGCT 2 cut(s) 181, 413
Alw26I GTCTC 2 cut(s) 301, 375
AlwI GGATC 1 cut(s) 98
AoxI GGCC 2 cut(s) 117, 264
ApeKI GCWGC 2 cut(s) 410, 422
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 260
AvaII GGWCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 278
BbvI GCAGC 2 cut(s) 422, 434
BciT130I CCWGG 1 cut(s) 198
BcnI CCSGG 1 cut(s) 260
BcoDI GTCTC 2 cut(s) 301, 375
BfmI CTRYAG 1 cut(s) 420
BisI GCNGC 3 cut(s) 264, 411, 423
BlsI GCNGC 3 cut(s) 265, 412, 424
Bme1390I CCNGG 2 cut(s) 198, 260
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmrFI CCNGG 2 cut(s) 198, 260
BpiI GAAGAC 1 cut(s) 278
BpmI CTGGAG 1 cut(s) 219
BpuMI CCSGG 1 cut(s) 260
Bsc4I CCNNNNNNNGG 1 cut(s) 162
Bse1I ACTGG 1 cut(s) 78
BseBI CCWGG 1 cut(s) 198
BseLI CCNNNNNNNGG 1 cut(s) 162
BseNI ACTGG 1 cut(s) 78
BseRI GAGGAG 1 cut(s) 363
BseXI GCAGC 2 cut(s) 422, 434
Bsh1285I CGRYCG 1 cut(s) 243
BshFI GGCC 2 cut(s) 119, 266
BsiEI CGRYCG 1 cut(s) 243
BsiSI CCGG 2 cut(s) 142, 260
BslI CCNNNNNNNGG 1 cut(s) 162
BsmAI GTCTC 2 cut(s) 301, 375
BsmBI CGTCTC 1 cut(s) 375
BsnI GGCC 2 cut(s) 119, 266
Bsp143I GATC 4 cut(s) 49, 90, 240, 289
BspACI CCGC 1 cut(s) 263
BspANI GGCC 2 cut(s) 119, 266
BspMAI CTGCAG 1 cut(s) 424
BspPI GGATC 1 cut(s) 98
BsrI ACTGG 1 cut(s) 78
BssMI GATC 4 cut(s) 49, 90, 240, 289
Bst2UI CCWGG 1 cut(s) 198
Bst4CI ACNGT 1 cut(s) 210
BstKTI GATC 4 cut(s) 52, 93, 243, 292
BstMAI GTCTC 2 cut(s) 301, 375
BstMBI GATC 4 cut(s) 49, 90, 240, 289
BstMCI CGRYCG 1 cut(s) 243
BstMWI GCNNNNNNNGC 1 cut(s) 419
BstNI CCWGG 1 cut(s) 198
BstNSI RCATGY 2 cut(s) 30, 222
BstSCI CCNGG 2 cut(s) 196, 258
BstSFI CTRYAG 1 cut(s) 420
BstV1I GCAGC 2 cut(s) 422, 434
BstV2I GAAGAC 1 cut(s) 278
BstX2I RGATCY 1 cut(s) 90
BstYI RGATCY 1 cut(s) 90
BsuRI GGCC 2 cut(s) 119, 266
BtsIMutI CAGTG 1 cut(s) 85
Cfr13I GGNCC 1 cut(s) 4
CviAII CATG 3 cut(s) 27, 219, 342
CviJI RGCY 5 cut(s) 119, 181, 249, 266, 413
CviKI_1 RGCY 5 cut(s) 119, 181, 249, 266, 413
DpnI GATC 4 cut(s) 51, 92, 242, 291
DpnII GATC 4 cut(s) 49, 90, 240, 289
DraI TTTAAA 1 cut(s) 70
DrdI GACNNNNNNGTC 1 cut(s) 315
DseDI GACNNNNNNGTC 1 cut(s) 315
EaeI YGGCCR 2 cut(s) 117, 264
Eco32I GATATC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 196
EcoRV GATATC 1 cut(s) 150
Esp3I CGTCTC 1 cut(s) 375
FaeI CATG 3 cut(s) 30, 222, 345
FatI CATG 3 cut(s) 26, 218, 341
Fnu4HI GCNGC 3 cut(s) 264, 411, 423
Fsp4HI GCNGC 3 cut(s) 264, 411, 423
GluI GCNGC 3 cut(s) 264, 411, 423
GsuI CTGGAG 1 cut(s) 219
HaeIII GGCC 2 cut(s) 119, 266
HapII CCGG 2 cut(s) 142, 260
Hin1II CATG 3 cut(s) 30, 222, 345
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HinfI GANTC 1 cut(s) 350
HpaI GTTAAC 1 cut(s) 213
HpaII CCGG 2 cut(s) 142, 260
Hpy166II GTNNAC 1 cut(s) 213
Hpy188I TCNGA 2 cut(s) 165, 359
Hpy188III TCNNGA 1 cut(s) 94
Hpy8I GTNNAC 1 cut(s) 213
Hpy99I CGWCG 1 cut(s) 125
HpyCH4III ACNGT 1 cut(s) 210
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 5 cut(s) 30, 101, 335, 410, 422
HpyF10VI GCNNNNNNNGC 1 cut(s) 419
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 3 cut(s) 30, 222, 345
KspAI GTTAAC 1 cut(s) 213
Kzo9I GATC 4 cut(s) 49, 90, 240, 289
LpnPI CCDG 7 cut(s) 54, 91, 155, 183, 210, 273, 288
Lsp1109I GCAGC 2 cut(s) 422, 434
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 317
MalI GATC 4 cut(s) 51, 92, 242, 291
MboI GATC 4 cut(s) 49, 90, 240, 289
MboII GAAGA 1 cut(s) 283
MfeI CAATTG 1 cut(s) 437
MflI RGATCY 1 cut(s) 90
MluCI AATT 4 cut(s) 171, 276, 312, 437
MnlI CCTC 4 cut(s) 13, 340, 353, 384
MseI TTAA 2 cut(s) 69, 212
MspI CCGG 2 cut(s) 142, 260
MspR9I CCNGG 2 cut(s) 198, 260
MunI CAATTG 1 cut(s) 437
MvaI CCWGG 1 cut(s) 198
MwoI GCNNNNNNNGC 1 cut(s) 419
NciI CCSGG 1 cut(s) 260
NdeII GATC 4 cut(s) 49, 90, 240, 289
NlaIII CATG 3 cut(s) 30, 222, 345
NmuCI GTSAC 1 cut(s) 317
NspI RCATGY 2 cut(s) 30, 222
PciI ACATGT 1 cut(s) 218
PfeI GAWTC 1 cut(s) 350
PkrI GCNGC 3 cut(s) 265, 412, 424
Ple19I CGATCG 1 cut(s) 243
PscI ACATGT 1 cut(s) 218
Psp6I CCWGG 1 cut(s) 196
PspGI CCWGG 1 cut(s) 196
PspPI GGNCC 1 cut(s) 4
PstI CTGCAG 1 cut(s) 424
PsuI RGATCY 1 cut(s) 90
PvuI CGATCG 1 cut(s) 243
SaqAI TTAA 2 cut(s) 69, 212
SatI GCNGC 3 cut(s) 264, 411, 423
Sau3AI GATC 4 cut(s) 49, 90, 240, 289
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 2 cut(s) 198, 260
SetI ASST 6 cut(s) 126, 183, 199, 300, 408, 415
SfcI CTRYAG 1 cut(s) 420
SinI GGWCC 1 cut(s) 4
Sse9I AATT 4 cut(s) 171, 276, 312, 437
SsiI CCGC 1 cut(s) 263
StyD4I CCNGG 2 cut(s) 196, 258
TaaI ACNGT 1 cut(s) 210
TaiI ACGT 1 cut(s) 126
TaqI TCGA 1 cut(s) 74
TasI AATT 4 cut(s) 171, 276, 312, 437
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 1 cut(s) 350
Tru1I TTAA 2 cut(s) 69, 212
Tru9I TTAA 2 cut(s) 69, 212
TscAI CASTG 1 cut(s) 85
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 2 cut(s) 410, 422
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 1 cut(s) 342
TspGWI ACGGA 1 cut(s) 357
TspRI CASTG 1 cut(s) 85
VpaK11BI GGWCC 1 cut(s) 4
XceI RCATGY 2 cut(s) 30, 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.