Rmu_sc0007101.1_g000011

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007101.1
OG0004594
Physical Location & Seq
Forward (+)
48720 .. 55545
6826 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1686 bp
atgctggtgctcggcgagtacgacgacctgagtgcggtggaaacagtgccgttgcagatggtggaaacttggattaggatcaagggcttacctccatgtatgaggacggagaaaaccctaatcttcattggttcctcgatggggaagttcctgaaggcagatccattacctttgcatcatgtgtggcagagcaatggttcaaaggctaagtggacgtggttcacgttcaattttggtattttggcggccagtatgagaagtttaaggggttgtgctgggattgtggctatttttcccatgaagtggagggatgtgacagaccaattgctccaaattgaagggcaaggagaagcaggggcctgccccgggactagggtggttgtggaagctgcagttgtggagacacaagcttttcctcccagggtctcaagggtccgtcgagaggcccaatctgcattagactgggtaaaaaaaatgcccaagctagtggatgggaccatcttagcctcgttgagttttgaacctggttttgatctcccaaacaccaatggggtgatttacttgtcccctaagaaacgaggccgtccaaaaggtagcgggaatgtagctaaaccgattcttggcgtccccaaacccaagaagaagcggttccctaaaaaagtagtgaagaagatggtggaggaggcagtgttgaactcgtcttcaaaagtcataagaagaagaaaagggccaccgccaaaactaaagagagaagccccaaagccatcgtctttagctttaattggatggtttgaggaatttcaagcagtttttgtgctccagagtactttgtttttgatggagaggttgtgctgtttcagtgcttccaattctcagtatggaggacggtcatcgtcgagctctggtaagggaaaaaacaatgaggggttaattaagtatggtttcagcctggtaaaaggaaaagctaatcatccaatggaggattatcatgttgctaagttcgttagggcccagggacatgagttaggattgtttgctatatatgatggccatttgggagacaccgtgcctcaatacttacagaagcatttgtttccaaatattcttaaggaggaagaattttgggtcgaccctaacagatctatctcaaaagcctatgagaggacagaccaggcaattctctcacatagcactgacttggggcgtggcgggtccactgctgtcactgcaatattgttaaatggtcagcggttatatgtggccaacgtcggagattcgcgggcagttctttcaaaggagggtcgggcagtacagatgtcaatagaccatgaacccaatactgaacgaggcagcattgaaaacaaaggtggatttgtctcaaacatgccaggagatgtacctagagttaatgggcagctggcagtttctcgtgcttttggagacaaaagcctcaagtcgcatttacgatcagacccagacatacaggatacttacatagacatgaatacagatgttctaatccttgccagtgatggcctttggaaggtaatgggtaatcaagaggcagttgatattgcaagaaaaattaaagaccctatgaaggccgctaaggagttaacagctgaagctttgaaaagagatagtaaagatgatatatcttgcgttgttgttaggtttaggggatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

561

Amino Acids

61.95

Weight (kDa)

9.59

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 321 - 552 6.2e-56 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 303
AccI GTMKAC 1 cut(s) 1128
AccII CGCG 1 cut(s) 1279
AciI CCGC 9 cut(s) 35, 245, 597, 646, 734, 1209, 1249, 1279, 1605
AclWI GGATC 2 cut(s) 86, 155
AcoI YGGCCR 3 cut(s) 246, 1048, 1260
AcsI RAATTY 2 cut(s) 797, 1118
AcuI CTGAAG 2 cut(s) 173, 1644
AcyI GRCGYC 1 cut(s) 624
AfaI GTAC 4 cut(s) 20, 826, 1311, 1398
AflII CTTAAG 1 cut(s) 1106
AjiI CACGTC 1 cut(s) 216
AjnI CCWGG 6 cut(s) 419, 523, 948, 1011, 1170, 1387
Alw21I GWGCWC 3 cut(s) 12, 819, 902
Alw26I GTCTC 5 cut(s) 395, 430, 1053, 1381, 1434
AlwI GGATC 2 cut(s) 86, 155
Ama87I CYCGRG 1 cut(s) 365
ApaI GGGCCC 1 cut(s) 1012
ApeKI GCWGC 3 cut(s) 389, 1350, 1414
ApoI RAATTY 2 cut(s) 797, 1118
ArsI GACNNNNNNTTYG 2 cut(s) 752, 784
Asp700I GAANNNNTTC 1 cut(s) 647
AspS9I GGNCC 8 cut(s) 357, 433, 445, 495, 728, 1008, 1009, 1212
AsuC2I CCSGG 2 cut(s) 366, 367
AsuHPI GGTGA 1 cut(s) 565
AvaI CYCGRG 1 cut(s) 365
AvaII GGWCC 3 cut(s) 433, 495, 1212
BaeGI GKGCMC 1 cut(s) 1012
BalI TGGCCA 2 cut(s) 1050, 1262
BanII GRGCYC 2 cut(s) 902, 1012
BauI CACGAG 1 cut(s) 1428
BbsI GAAGAC 1 cut(s) 693
Bbv12I GWGCWC 3 cut(s) 12, 819, 902
BbvI GCAGC 3 cut(s) 376, 1362, 1426
BceAI ACGGC 2 cut(s) 34, 567
BcgI CGANNNNNNTGC 2 cut(s) 14, 48
BciT130I CCWGG 6 cut(s) 421, 525, 950, 1013, 1172, 1389
BciVI GTATCC 1 cut(s) 1480
BcnI CCSGG 2 cut(s) 366, 367
BcoDI GTCTC 5 cut(s) 395, 430, 1053, 1381, 1434
BfaI CTAG 3 cut(s) 372, 485, 1401
BfmI CTRYAG 1 cut(s) 390
BfrI CTTAAG 1 cut(s) 1106
BfuI GTATCC 1 cut(s) 1480
BglII AGATCT 1 cut(s) 1139
BisI GCNGC 5 cut(s) 246, 390, 1351, 1415, 1605
BlsI GCNGC 5 cut(s) 247, 391, 1352, 1416, 1606
BmcAI AGTACT 1 cut(s) 826
Bme1390I CCNGG 8 cut(s) 366, 367, 421, 525, 950, 1013, 1172, 1389
Bme18I GGWCC 3 cut(s) 433, 495, 1212
BmeT110I CYCGRG 1 cut(s) 365
BmgBI CACGTC 1 cut(s) 216
BmgT120I GGNCC 8 cut(s) 357, 433, 445, 495, 728, 1008, 1009, 1212
BmiI GGNNCC 7 cut(s) 133, 358, 434, 496, 650, 1010, 1213
BmrFI CCNGG 8 cut(s) 366, 367, 421, 525, 950, 1013, 1172, 1389
BmrI ACTGGG 1 cut(s) 472
BmsI GCATC 1 cut(s) 184
BmuI ACTGGG 1 cut(s) 472
BpiI GAAGAC 1 cut(s) 693
BpmI CTGGAG 1 cut(s) 803
Bpu10I CCTNAGC 1 cut(s) 1608
BpuEI CTTGAG 2 cut(s) 412, 1436
BpuMI CCSGG 2 cut(s) 366, 367
BsaBI GATNNNNATC 1 cut(s) 77
BsaHI GRCGYC 1 cut(s) 624
BsaI GGTCTC 1 cut(s) 430
BsaJI CCNNGG 6 cut(s) 364, 365, 419, 420, 1011, 1012
BsaXI ACNNNNNCTCC 6 cut(s) 674, 704, 833, 863, 1050, 1080
Bse1I ACTGG 3 cut(s) 249, 467, 1527
Bse3DI GCAATG 1 cut(s) 199
Bse8I GATNNNNATC 1 cut(s) 77
BseBI CCWGG 6 cut(s) 421, 525, 950, 1013, 1172, 1389
BseDI CCNNGG 6 cut(s) 364, 365, 419, 420, 1011, 1012
BseGI GGATG 4 cut(s) 316, 496, 791, 970
BseJI GATNNNNATC 1 cut(s) 77
BseMI GCAATG 1 cut(s) 199
BseMII CTCAG 2 cut(s) 20, 887
BseNI ACTGG 3 cut(s) 249, 467, 1527
BseRI GAGGAG 1 cut(s) 695
BseSI GKGCMC 1 cut(s) 1012
BseXI GCAGC 3 cut(s) 376, 1362, 1426
BseYI CCCAGC 1 cut(s) 275
Bsh1236I CGCG 1 cut(s) 1279
BsiHKAI GWGCWC 3 cut(s) 12, 819, 902
BsiHKCI CYCGRG 1 cut(s) 365
BsiSI CCGG 1 cut(s) 366
BslFI GGGAC 5 cut(s) 382, 508, 550, 611, 1029
BsmAI GTCTC 5 cut(s) 395, 430, 1053, 1381, 1434
BsmFI GGGAC 5 cut(s) 382, 508, 550, 611, 1029
Bso31I GGTCTC 1 cut(s) 430
BsoBI CYCGRG 1 cut(s) 365
Bsp120I GGGCCC 1 cut(s) 1008
Bsp1286I GDGCHC 4 cut(s) 12, 819, 902, 1012
Bsp143I GATC 5 cut(s) 78, 160, 532, 1139, 1466
BspACI CCGC 9 cut(s) 35, 245, 597, 646, 734, 1209, 1249, 1279, 1605
BspCNI CTCAG 2 cut(s) 21, 886
BspFNI CGCG 1 cut(s) 1279
BspLI GGNNCC 7 cut(s) 133, 358, 434, 496, 650, 1010, 1213
BspMAI CTGCAG 1 cut(s) 394
BspPI GGATC 2 cut(s) 86, 155
BspTI CTTAAG 1 cut(s) 1106
BspTNI GGTCTC 1 cut(s) 430
BsrDI GCAATG 1 cut(s) 199
BsrI ACTGG 3 cut(s) 249, 467, 1527
BssECI CCNNGG 6 cut(s) 364, 365, 419, 420, 1011, 1012
BssMI GATC 5 cut(s) 78, 160, 532, 1139, 1466
BssNI GRCGYC 1 cut(s) 624
BssSI CACGAG 1 cut(s) 1428
Bst2BI CACGAG 1 cut(s) 1428
Bst2UI CCWGG 6 cut(s) 421, 525, 950, 1013, 1172, 1389
Bst4CI ACNGT 3 cut(s) 46, 888, 1066
BstACI GRCGYC 1 cut(s) 624
BstAFI CTTAAG 1 cut(s) 1106
BstC8I GCNNGC 3 cut(s) 361, 1281, 1419
BstDEI CTNAG 7 cut(s) 29, 207, 502, 570, 873, 996, 1608
BstENI CCTNNNNNAGG 2 cut(s) 1159, 1541
BstF5I GGATG 4 cut(s) 316, 496, 791, 970
BstFNI CGCG 1 cut(s) 1279
BstKTI GATC 5 cut(s) 81, 163, 535, 1142, 1469
BstMAI GTCTC 5 cut(s) 395, 430, 1053, 1381, 1434
BstMBI GATC 5 cut(s) 78, 160, 532, 1139, 1466
BstMWI GCNNNNNNNGC 2 cut(s) 452, 1226
BstNI CCWGG 6 cut(s) 421, 525, 950, 1013, 1172, 1389
BstNSI RCATGY 1 cut(s) 1387
BstSCI CCNGG 8 cut(s) 364, 365, 419, 523, 948, 1011, 1170, 1387
BstSFI CTRYAG 1 cut(s) 390
BstSLI GKGCMC 1 cut(s) 1012
BstUI CGCG 1 cut(s) 1279
BstV1I GCAGC 3 cut(s) 376, 1362, 1426
BstV2I GAAGAC 1 cut(s) 693
BstX2I RGATCY 2 cut(s) 160, 1139
BstXI CCANNNNNNTGG 1 cut(s) 487
BstYI RGATCY 2 cut(s) 160, 1139
BsuI GTATCC 1 cut(s) 1480
BtrI CACGTC 1 cut(s) 216
BtsCI GGATG 4 cut(s) 316, 496, 791, 970
BtsI GCAGTG 3 cut(s) 693, 1215, 1224
BtsIMutI CAGTG 7 cut(s) 51, 693, 865, 1191, 1215, 1224, 1534
Cac8I GCNNGC 3 cut(s) 361, 1281, 1419
Cfr13I GGNCC 8 cut(s) 357, 433, 445, 495, 728, 1008, 1009, 1212
Cfr9I CCCGGG 1 cut(s) 365
CseI GACGC 1 cut(s) 613
CsiI ACCWGGT 1 cut(s) 523
Csp6I GTAC 4 cut(s) 19, 825, 1310, 1397
CviAII CATG 8 cut(s) 96, 179, 298, 989, 1019, 1328, 1384, 1501
CviQI GTAC 4 cut(s) 19, 825, 1310, 1397
DdeI CTNAG 7 cut(s) 29, 207, 502, 570, 873, 996, 1608
DpnI GATC 5 cut(s) 80, 162, 534, 1141, 1468
DpnII GATC 5 cut(s) 78, 160, 532, 1139, 1466
EaeI YGGCCR 3 cut(s) 246, 1048, 1260
Ecl136II GAGCTC 1 cut(s) 900
Eco24I GRGCYC 2 cut(s) 902, 1012
Eco31I GGTCTC 1 cut(s) 430
Eco47I GGWCC 3 cut(s) 433, 495, 1212
Eco53kI GAGCTC 1 cut(s) 900
Eco57I CTGAAG 2 cut(s) 173, 1644
Eco88I CYCGRG 1 cut(s) 365
EcoICRI GAGCTC 1 cut(s) 900
EcoNI CCTNNNNNAGG 2 cut(s) 1159, 1541
EcoO109I RGGNCCY 2 cut(s) 357, 1008
EcoRII CCWGG 6 cut(s) 419, 523, 948, 1011, 1170, 1387
EcoT38I GRGCYC 2 cut(s) 902, 1012
FaeI CATG 8 cut(s) 99, 182, 301, 992, 1022, 1331, 1387, 1504
FaqI GGGAC 5 cut(s) 382, 508, 550, 611, 1029
FatI CATG 8 cut(s) 95, 178, 297, 988, 1018, 1327, 1383, 1500
FauI CCCGC 3 cut(s) 590, 1202, 1272
FblI GTMKAC 1 cut(s) 1128
Fnu4HI GCNGC 5 cut(s) 246, 390, 1351, 1415, 1605
FokI GGATG 4 cut(s) 323, 503, 798, 957
FriOI GRGCYC 2 cut(s) 902, 1012
Fsp4HI GCNGC 5 cut(s) 246, 390, 1351, 1415, 1605
FspBI CTAG 3 cut(s) 372, 485, 1401
GluI GCNGC 5 cut(s) 246, 390, 1351, 1415, 1605
GsaI CCCAGC 1 cut(s) 279
GsuI CTGGAG 1 cut(s) 803
HapII CCGG 1 cut(s) 366
HgaI GACGC 1 cut(s) 613
Hin1I GRCGYC 1 cut(s) 624
Hin1II CATG 8 cut(s) 99, 182, 301, 992, 1022, 1331, 1387, 1504
HincII GTYRAC 2 cut(s) 1129, 1617
HindII GTYRAC 2 cut(s) 1129, 1617
HindIII AAGCTT 2 cut(s) 408, 1626
HinfI GANTC 2 cut(s) 616, 1274
HpaI GTTAAC 1 cut(s) 1617
HpaII CCGG 1 cut(s) 366
HphI GGTGA 1 cut(s) 565
Hpy166II GTNNAC 5 cut(s) 213, 222, 1129, 1215, 1617
Hpy188I TCNGA 2 cut(s) 1271, 1471
Hpy188III TCNNGA 4 cut(s) 151, 440, 820, 1559
Hpy8I GTNNAC 5 cut(s) 213, 222, 1129, 1215, 1617
Hpy99I CGWCG 4 cut(s) 26, 441, 898, 1271
HpyAV CCTTC 4 cut(s) 148, 332, 1537, 1594
HpyCH4III ACNGT 3 cut(s) 46, 888, 1066
HpyCH4IV ACGT 3 cut(s) 215, 224, 1266
HpyCH4V TGCA 6 cut(s) 55, 175, 392, 455, 1229, 1577
HpyF10VI GCNNNNNNNGC 2 cut(s) 452, 1226
HpyF3I CTNAG 7 cut(s) 29, 207, 502, 570, 873, 996, 1608
HpySE526I ACGT 3 cut(s) 215, 224, 1266
Hsp92I GRCGYC 1 cut(s) 624
Hsp92II CATG 8 cut(s) 99, 182, 301, 992, 1022, 1331, 1387, 1504
KspAI GTTAAC 1 cut(s) 1617
Kzo9I GATC 5 cut(s) 78, 160, 532, 1139, 1466
LmnI GCTCC 2 cut(s) 333, 822
Lsp1109I GCAGC 3 cut(s) 376, 1362, 1426
LweI GCATC 1 cut(s) 184
MabI ACCWGGT 1 cut(s) 523
MaeI CTAG 3 cut(s) 372, 485, 1401
MaeII ACGT 3 cut(s) 215, 224, 1266
MaeIII GTNAC 2 cut(s) 313, 1222
MalI GATC 5 cut(s) 80, 162, 534, 1141, 1468
MboI GATC 5 cut(s) 78, 160, 532, 1139, 1466
MboII GAAGA 8 cut(s) 115, 652, 679, 682, 693, 729, 732, 1127
MfeI CAATTG 1 cut(s) 323
MflI RGATCY 2 cut(s) 160, 1139
MhlI GDGCHC 4 cut(s) 12, 819, 902, 1012
MlsI TGGCCA 2 cut(s) 1050, 1262
MluNI TGGCCA 2 cut(s) 1050, 1262
MmeI TCCRAC 1 cut(s) 1249
Mox20I TGGCCA 2 cut(s) 1050, 1262
MroXI GAANNNNTTC 1 cut(s) 647
MscI TGGCCA 2 cut(s) 1050, 1262
MseI TTAA 9 cut(s) 263, 779, 929, 933, 1107, 1238, 1407, 1587, 1616
MslI CAYNNNNRTG 1 cut(s) 1499
Msp20I TGGCCA 2 cut(s) 1050, 1262
MspA1I CMGCKG 3 cut(s) 1249, 1417, 1622
MspCI CTTAAG 1 cut(s) 1106
MspI CCGG 1 cut(s) 366
MspR9I CCNGG 8 cut(s) 366, 367, 421, 525, 950, 1013, 1172, 1389
MunI CAATTG 1 cut(s) 323
MvaI CCWGG 6 cut(s) 421, 525, 950, 1013, 1172, 1389
MvnI CGCG 1 cut(s) 1279
MwoI GCNNNNNNNGC 2 cut(s) 452, 1226
NciI CCSGG 2 cut(s) 366, 367
NdeII GATC 5 cut(s) 78, 160, 532, 1139, 1466
NlaIII CATG 8 cut(s) 99, 182, 301, 992, 1022, 1331, 1387, 1504
NlaIV GGNNCC 7 cut(s) 133, 358, 434, 496, 650, 1010, 1213
NmuCI GTSAC 2 cut(s) 313, 1222
NspI RCATGY 1 cut(s) 1387
PacI TTAATTAA 1 cut(s) 933
PasI CCCWGGG 2 cut(s) 420, 1012
PcsI WCGNNNNNNNCGW 2 cut(s) 18, 221
PdmI GAANNNNTTC 1 cut(s) 647
PfeI GAWTC 2 cut(s) 616, 1274
PflMI CCANNNNNTGG 1 cut(s) 303
PkrI GCNGC 5 cut(s) 247, 391, 1352, 1416, 1606
Psp124BI GAGCTC 1 cut(s) 902
Psp6I CCWGG 6 cut(s) 419, 523, 948, 1011, 1170, 1387
PspFI CCCAGC 1 cut(s) 275
PspGI CCWGG 6 cut(s) 419, 523, 948, 1011, 1170, 1387
PspN4I GGNNCC 7 cut(s) 133, 358, 434, 496, 650, 1010, 1213
PspOMI GGGCCC 1 cut(s) 1008
PspPI GGNCC 8 cut(s) 357, 433, 445, 495, 728, 1008, 1009, 1212
PstI CTGCAG 1 cut(s) 394
PsuI RGATCY 2 cut(s) 160, 1139
PvuII CAGCTG 2 cut(s) 1417, 1622
RsaI GTAC 4 cut(s) 20, 826, 1311, 1398
RsaNI GTAC 4 cut(s) 19, 825, 1310, 1397
RseI CAYNNNNRTG 1 cut(s) 1499
SacI GAGCTC 1 cut(s) 902
SalI GTCGAC 1 cut(s) 1127
SaqAI TTAA 9 cut(s) 263, 779, 929, 933, 1107, 1238, 1407, 1587, 1616
SatI GCNGC 5 cut(s) 246, 390, 1351, 1415, 1605
Sau3AI GATC 5 cut(s) 78, 160, 532, 1139, 1466
Sau96I GGNCC 8 cut(s) 357, 433, 445, 495, 728, 1008, 1009, 1212
ScaI AGTACT 1 cut(s) 826
ScrFI CCNGG 8 cut(s) 366, 367, 421, 525, 950, 1013, 1172, 1389
SduI GDGCHC 4 cut(s) 12, 819, 902, 1012
SexAI ACCWGGT 1 cut(s) 523
SfaNI GCATC 1 cut(s) 184
SfcI CTRYAG 1 cut(s) 390
SinI GGWCC 3 cut(s) 433, 495, 1212
SmaI CCCGGG 1 cut(s) 367
SmiMI CAYNNNNRTG 1 cut(s) 1499
SmlI CTYRAG 3 cut(s) 427, 1106, 1451
SmoI CTYRAG 3 cut(s) 427, 1106, 1451
SsiI CCGC 9 cut(s) 35, 245, 597, 646, 734, 1209, 1249, 1279, 1605
SspI AATATT 2 cut(s) 1102, 1233
SspMI CTAG 3 cut(s) 372, 485, 1401
SstI GAGCTC 1 cut(s) 902
StyD4I CCNGG 8 cut(s) 364, 365, 419, 523, 948, 1011, 1170, 1387
TaaI ACNGT 3 cut(s) 46, 888, 1066
TaiI ACGT 3 cut(s) 218, 227, 1269
TaqI TCGA 4 cut(s) 137, 439, 896, 1128
TatI WGTACW 2 cut(s) 824, 1309
TauI GCSGC 2 cut(s) 248, 1607
TfiI GAWTC 2 cut(s) 616, 1274
Tru1I TTAA 9 cut(s) 263, 779, 929, 933, 1107, 1238, 1407, 1587, 1616
Tru9I TTAA 9 cut(s) 263, 779, 929, 933, 1107, 1238, 1407, 1587, 1616
TscAI CASTG 7 cut(s) 51, 693, 865, 1198, 1222, 1231, 1534
TseFI GTSAC 2 cut(s) 313, 1222
TseI GCWGC 3 cut(s) 389, 1350, 1414
Tsp45I GTSAC 2 cut(s) 313, 1222
TspDTI ATGAA 5 cut(s) 115, 314, 1344, 1517, 1613
TspGWI ACGGA 2 cut(s) 122, 425
TspMI CCCGGG 1 cut(s) 365
TspRI CASTG 7 cut(s) 51, 693, 865, 1198, 1222, 1231, 1534
Van91I CCANNNNNTGG 1 cut(s) 303
Vha464I CTTAAG 1 cut(s) 1106
VpaK11BI GGWCC 3 cut(s) 433, 495, 1212
XagI CCTNNNNNAGG 2 cut(s) 1159, 1541
XapI RAATTY 2 cut(s) 797, 1118
XceI RCATGY 1 cut(s) 1387
XmaI CCCGGG 1 cut(s) 365
XmiI GTMKAC 1 cut(s) 1128
XmnI GAANNNNTTC 1 cut(s) 647
XspI CTAG 3 cut(s) 372, 485, 1401
ZrmI AGTACT 1 cut(s) 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.