Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0007283.1

Orthogroup ID

OG0003293

Physical Location & Seq

Genomic Coordinates Reverse (-)

18963 .. 26169

Gene Structure 7207 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3618 bp

atggagagctatttgaacgagaactttgatttgaaggcgaagaactcatcggatgaggcgctccagagatggaggaagctctgctgggttgtcaagaataagaaacggaggtttcgattcactgctaatctcgagaagcgttccgaggcccagactcttcgacgctctaatcaggagaagtttagacttgcaatcttggtttcgcaagctgcacttcagttcatccaaggactaaaatttttcagtgactacactgtacctgaggaagtcaaagctgcaggttttcaaattggtgctgatgagttggcatccattgttgaaggtcgtgatgtgaagaagctcaaaatgcatgatggtgttatgggtctagtaggcaagcttggaacatcaattgttgatggcatttcaacctctgagcaattgatcaatgcaagaaaggaaatttatggagttaataagttcactgagagcccatctcgtggatttttactatttgtatgggaagcccttcaagacaccaccctcatgatacttggagtttgtgcctttgtctctctgcttgttgggataatgacagaaggatggccaaagggtgcccatgatggacttggaattgttgcgagcattttgcttgttgtatttgtgactgctactagcgattataagcagtccctgcagttcaaggacttggacaaggagaagaaaaagattacagttcatgttactagaaatggattcagacagaagttgtcaatatatgatttacttcctggtgacgttgttcatctcaatattggagatctggtcccagctgatggacttttcatttctggcttttctatgctgataaatgaatccagcttaactggtgagagtgaaccggttaatattaatgatgtaaatccttttctcctctctggaactaaagttcaagatggatcctgcaagatgcttgtgactactgttggaatgagaacccagtggggtaaattgatggctactcttactgaaggtggagatgatgaaacccccttgcaggtgaaactaaacggtgtggcaactatcattggtaaaataggcctgttttttgcagtagtgacatttgctgtattagttcaaggattgttcagccgcaagcttcaagaaggattagtatggtccggagatgatgcattggtaattttggaatttttcgctatagctgttacaattgtagttgttgccgttcctgaaggtttgcctttggctgtaacattaagccttgcttttgccatgaagaagatgatgaatgataaggcacttgtccgacatttggctgcttgtgagacaatgggatctgccacaactatctgtagtgacaagactggaacactaacaactaaccggatgacggttgtaaaaacttgcatttgtttgaaaatcaaaaatgtggctagctctgcgaaatcatctgacctctgctctgaaatacctgatgctactttgaaagttctacttcagtgcatatttaacaatactggaggagacattgttaaaaacaaagatgacaggattgagatactgggaacacccactgacactgctgttttgggatttgggatgtcactaggtggagatttcaaggcagaacgacaagcatcaaaacttgtgaaagttgagcccttcaattctgtgaagaagcggatggctgttgttctacagcttcctgaaggtggcttccgagtgcattgtaaaggtgcttctgagatcattttagctacatgtgacaaatggatgggtccaaatggggaggtaattccccttgatatagcttccatcaatcttttgaatggtatcatagaacagtttgctagtgaagcccttagaactctatgcctggcatacatggacattggaagtgagttctctgctgagagtcctattcctgtcaatggatatacatgtataggaattgtgggtattaaagatccagttcgtcctggtgtcaaggagtctgttgagatttgtaggtctgctggtattactgtccgaatggttaccggtgataacataaacactgcaaaagcaattgcaagagaatgtggaattttgactgatggaggtttagcaattgaaggcccagaatttcgtgaaaagagtgaggaggaattacaaaaaattattccaaaacttcaggtaatggctcgatcttcgccaatggataagcatacccttgtaaaacacttgagaaccacttttggagaagttgttgcagtgacaggtgatggtacaaatgatgctccagcacttcatgaagctgatataggacttgcaatgggaattgctggaactgaggtggctaaagagagtgctgatgtaataattctggatgacaacttctctacaattgtgaccgtggctaaatgggggcgttcagtttacttgaacatacagaaatttgttcagttccagctaacagttaatgtggttgctcttgtagtcaacttctcttcagcatgtttaactggaagcgctccccttactgctgttcagcttctctgggtcaacatgatcatggacactctaggagcccttgcattagccactgagccccctaatgatgatttgatgaagagaacaccagttggcaggacaggaaacttcattactaatgtgatgtggagaaatatcttggggcagtcgctgtatcagtttgtggtaatatggtatctgctgaccagaggaaaagaagcttttcagcttgttggcccagattcagatttgatattaaacaccctgattttcaactcatttgtcttctgtcaggctttcaatgagatcagttgtagagaaatggagaagatcaatgtcttcaaaggcatactgcagaattatgtgtttgtgactgttctcttctgcactataatcttccaagttataatcatcgagttcctgggtacatttgcaagcacatctcctctcacttggcagcagtggtttgtcagtgttacacttgggttcctgggcatgcctatttcagcagctttgaaatttattcctgtaatcggctgtggaaccaccggaacaggccggaaaaccactgaaccagccatcggaaccacaaccgccgcatatattgaagcccttaatcccaggattccagaatcggctgtggaaccaccggaacaggccggaaaaccactgaaccagccatcggaagtatcgaaccagccctccaagaagaaaaaaaggggatcacccggttcagaagcttctagactgcctttcatcatcaaacttggccatcattgccaacctgaccccaggatccaggaaccggagaaaaaaaggggatcacccggttcagaagcttctagactgcctttcatcatcaaacttggccatcattgccaacctgaccctaggatccaggaaccgggattagattacctccccagaaactggccacaatgtgacgcgaccgacagtaactccgtcagggagaaaataagaaaaaaaaaccagaaaaataaaagaaagcagcccaagagagaaggagaggctcgcaacccaaaagcccacagagccaggcccactgacgcacctaacccactaccacgtcagatcgtgccacattag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009706 GO:0009941 GO:0012505 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0019866 GO:0022803 GO:0022804 GO:0022838 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0031967 GO:0031969 GO:0031975 GO:0034220 GO:0042170 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1205

Amino Acids

131.84

Weight (kDa)

8.59

Isoelectric Point (pI)

40.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	5 - 49	1.9e-20	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	119 - 185	4.8e-09	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	236 - 334	1.1e-19	P-type ATPase actuator domain
Cation_ATPase	PF13246	501 - 660	5.5e-30	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	633 - 767	7.9e-12	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	838 - 1012	1.9e-41	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003293)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27770 AT1G27770 AT1G27770 AT1G27770
fragaria_vesca	FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180
malus_domestica	MD08G1085200.v1.1 MD08G1085600.v1.1 MD12G1068300.v1.1 MD15G1070800.v1.1
prunus_persica	Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1
pyrus_communis	pycom08g07020 pycom15g06650 pycom15g06660
rosa_chinensis	RchiOBHm_Chr6g0309491
rosa_laevigata	RLG00000010561
rosa_multiflora	Rmu_sc0007283.1_g000007
rosa_roxburghii	Rroxscaffold_7G00159360
rosa_rugosa	Rorug06G0372300 Rorug06G0372400
rosa_samantha	Rh6AG485700 Rh6BG495000
rosa_wichuraiana	Rw6G042300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	663, 2918
AarI	CACCTGC	1 cut(s)	1027
Acc36I	ACCTGC	2 cut(s)	269, 1027
AccB1I	GGYRCC	1 cut(s)	593
AccB7I	CCANNNNNTGG	3 cut(s)	479, 815, 3441
AccII	CGCG	1 cut(s)	3458
AccIII	TCCGGA	1 cut(s)	1160
AciI	CCGC	4 cut(s)	1132, 1681, 3105, 3108
AcoI	YGGCCR	4 cut(s)	584, 3280, 3379, 3443
AcsI	RAATTY	7 cut(s)	236, 441, 1187, 2085, 2123, 2435, 3029
AcuI	CTGAAG	7 cut(s)	200, 1029, 1251, 1481, 1728, 2156, 2475
AdeI	CACNNNGTG	2 cut(s)	1610, 3452
AfaI	GTAC	3 cut(s)	258, 2269, 2938
AfeI	AGCGCT	1 cut(s)	2512
AflIII	ACRYGT	2 cut(s)	1760, 1940
AgeI	ACCGGT	2 cut(s)	880, 2039
AjiI	CACGTC	1 cut(s)	3599
AloI	GAACNNNNNNTCC	4 cut(s)	912, 944, 3197, 3229
Alw26I	GTCTC	3 cut(s)	556, 1319, 1518
AlwNI	CAGNNNCTG	3 cut(s)	673, 2683, 3441
Ama87I	CYCGRG	1 cut(s)	131
Aor13HI	TCCGGA	1 cut(s)	1160
Aor51HI	AGCGCT	1 cut(s)	2512
ApeKI	GCWGC	6 cut(s)	209, 275, 1316, 2968, 3020, 3520
ApoI	RAATTY	7 cut(s)	236, 441, 1187, 2085, 2123, 2435, 3029
AseI	ATTAAT	1 cut(s)	891
AsiGI	ACCGGT	2 cut(s)	880, 2039
Asp700I	GAANNNNTTC	2 cut(s)	507, 2733
AspA2I	CCTAGG	1 cut(s)	3401
AspLEI	GCGC	2 cut(s)	61, 2513
AspS9I	GGNCC	7 cut(s)	148, 805, 1158, 1778, 2117, 2747, 3570
AsuC2I	CCSGG	3 cut(s)	3240, 3339, 3417
AsuHPI	GGTGA	7 cut(s)	785, 881, 1051, 2054, 2273, 3228, 3327
AsuNHI	GCTAGC	1 cut(s)	1433
AvaI	CYCGRG	1 cut(s)	131
AvaII	GGWCC	3 cut(s)	805, 1158, 1778
AvrII	CCTAGG	1 cut(s)	3401
AxyI	CCTNAGG	1 cut(s)	261
BaeGI	GKGCMC	1 cut(s)	598
BalI	TGGCCA	4 cut(s)	586, 3282, 3381, 3445
BamHI	GGATCC	3 cut(s)	938, 3306, 3405
BanI	GGYRCC	1 cut(s)	593
BanII	GRGCYC	4 cut(s)	473, 1662, 2572, 2592
BarI	GAAGNNNNNNTAC	2 cut(s)	1723, 1755
BauI	CACGAG	1 cut(s)	477
BbsI	GAAGAC	2 cut(s)	2788, 2842
BbvI	GCAGC	6 cut(s)	196, 262, 1303, 2980, 3032, 3532
BceAI	ACGGC	1 cut(s)	1208
BcgI	CGANNNNNNTGC	2 cut(s)	610, 644
BclI	TGATCA	2 cut(s)	423, 2550
BcnI	CCSGG	3 cut(s)	3240, 3339, 3417
BcoDI	GTCTC	3 cut(s)	556, 1319, 1518
BfmI	CTRYAG	6 cut(s)	276, 674, 1197, 1351, 1697, 2864
BfoI	RGCGCY	2 cut(s)	62, 2514
BfuAI	ACCTGC	2 cut(s)	269, 1027
BglII	AGATCT	1 cut(s)	799
BisI	GCNGC	8 cut(s)	210, 276, 1132, 1317, 2969, 3021, 3108, 3521
BlnI	CCTAGG	1 cut(s)	3401
BlsI	GCNGC	8 cut(s)	211, 277, 1133, 1318, 2970, 3022, 3109, 3522
Bme18I	GGWCC	3 cut(s)	805, 1158, 1778
BmeT110I	CYCGRG	1 cut(s)	131
BmgBI	CACGTC	1 cut(s)	3599
BmgT120I	GGNCC	7 cut(s)	148, 805, 1158, 1778, 2117, 2747, 3570
BmrI	ACTGGG	2 cut(s)	973, 1571
BmsI	GCATC	6 cut(s)	317, 939, 1159, 1465, 1646, 2266
BmtI	GCTAGC	1 cut(s)	1437
BmuI	ACTGGG	2 cut(s)	973, 1571
BpiI	GAAGAC	2 cut(s)	2788, 2842
BplI	GAGNNNNNCTC	2 cut(s)	460, 492
BpmI	CTGGAG	3 cut(s)	47, 1539, 2265
BpuEI	CTTGAG	1 cut(s)	2245
BpuMI	CCSGG	3 cut(s)	3240, 3339, 3417
BsaBI	GATNNNNATC	1 cut(s)	900
BsaJI	CCNNGG	8 cut(s)	144, 226, 2394, 2932, 3001, 3131, 3302, 3401
BsaWI	WCCGGW	7 cut(s)	880, 1160, 1383, 2039, 3059, 3160, 3316
Bse118I	RCCGGY	2 cut(s)	880, 2039
Bse1I	ACTGG	9 cut(s)	871, 979, 1369, 1522, 1566, 1970, 2509, 2621, 3446
Bse21I	CCTNAGG	1 cut(s)	261
Bse3DI	GCAATG	3 cut(s)	2319, 3286, 3385
Bse8I	GATNNNNATC	1 cut(s)	900
BseAI	TCCGGA	1 cut(s)	1160
BseDI	CCNNGG	8 cut(s)	144, 226, 2394, 2932, 3001, 3131, 3302, 3401
BseGI	GGATG	9 cut(s)	58, 222, 308, 587, 1392, 1605, 1689, 1779, 2374
BseJI	GATNNNNATC	1 cut(s)	900
BseMI	GCAATG	3 cut(s)	2319, 3286, 3385
BseMII	CTCAG	7 cut(s)	252, 405, 456, 1734, 1902, 2322, 2577
BseNI	ACTGG	9 cut(s)	871, 979, 1369, 1522, 1566, 1970, 2509, 2621, 3446
BseRI	GAGGAG	4 cut(s)	902, 1536, 2156, 2946
BseSI	GKGCMC	1 cut(s)	598
BseXI	GCAGC	6 cut(s)	196, 262, 1303, 2980, 3032, 3532
BseYI	CCCAGC	2 cut(s)	84, 808
BsgI	GTGCAG	2 cut(s)	195, 2881
Bsh1236I	CGCG	1 cut(s)	3458
Bsh1285I	CGRYCG	1 cut(s)	3462
BshNI	GGYRCC	1 cut(s)	593
BshTI	ACCGGT	2 cut(s)	880, 2039
BsiEI	CGRYCG	1 cut(s)	3462
BsiHKCI	CYCGRG	1 cut(s)	131
BslFI	GGGAC	2 cut(s)	655, 791
BsmAI	GTCTC	3 cut(s)	556, 1319, 1518
BsmFI	GGGAC	2 cut(s)	655, 791
BsoBI	CYCGRG	1 cut(s)	131
Bsp1286I	GDGCHC	5 cut(s)	473, 598, 1662, 2572, 2592
Bsp13I	TCCGGA	1 cut(s)	1160
BspACI	CCGC	4 cut(s)	1132, 1681, 3105, 3108
BspCNI	CTCAG	7 cut(s)	253, 406, 457, 1735, 1903, 2323, 2578
BspEI	TCCGGA	1 cut(s)	1160
BspFNI	CGCG	1 cut(s)	3458
BspHI	TCATGA	2 cut(s)	525, 2290
BspMAI	CTGCAG	3 cut(s)	280, 678, 2868
BspMI	ACCTGC	2 cut(s)	269, 1027
BspOI	GCTAGC	1 cut(s)	1437
BspT107I	GGYRCC	1 cut(s)	593
BsrDI	GCAATG	3 cut(s)	2319, 3286, 3385
BsrFI	RCCGGY	2 cut(s)	880, 2039
BsrI	ACTGG	9 cut(s)	871, 979, 1369, 1522, 1566, 1970, 2509, 2621, 3446
BssAI	RCCGGY	2 cut(s)	880, 2039
BssECI	CCNNGG	8 cut(s)	144, 226, 2394, 2932, 3001, 3131, 3302, 3401
BssSI	CACGAG	1 cut(s)	477
BssT1I	CCWWGG	2 cut(s)	226, 3401
Bst2BI	CACGAG	1 cut(s)	477
Bst6I	CTCTTC	4 cut(s)	162, 2494, 2606, 2897
BstAPI	GCANNNNNTGC	1 cut(s)	673
BstC8I	GCNNGC	8 cut(s)	207, 377, 622, 1136, 1435, 2947, 3008, 3544
BstDEI	CTNAG	8 cut(s)	261, 414, 465, 1743, 1862, 1911, 2331, 2586
BstDSI	CCRYGG	1 cut(s)	2394
BstEII	GGTNACC	1 cut(s)	2035
BstF5I	GGATG	9 cut(s)	58, 222, 308, 587, 1392, 1605, 1689, 1779, 2374
BstFNI	CGCG	1 cut(s)	3458
BstH2I	RGCGCY	2 cut(s)	62, 2514
BstHHI	GCGC	2 cut(s)	61, 2513
BstMAI	GTCTC	3 cut(s)	556, 1319, 1518
BstMCI	CGRYCG	1 cut(s)	3462
BstMWI	GCNNNNNNNGC	7 cut(s)	346, 673, 1439, 1856, 3288, 3387, 3563
BstNSI	RCATGY	4 cut(s)	1764, 1944, 2499, 3010
BstPI	GGTNACC	1 cut(s)	2035
BstSFI	CTRYAG	6 cut(s)	276, 674, 1197, 1351, 1697, 2864
BstSLI	GKGCMC	1 cut(s)	598
BstUI	CGCG	1 cut(s)	3458
BstV1I	GCAGC	6 cut(s)	196, 262, 1303, 2980, 3032, 3532
BstV2I	GAAGAC	2 cut(s)	2788, 2842
BstX2I	RGATCY	6 cut(s)	799, 938, 1334, 1966, 3306, 3405
BstYI	RGATCY	6 cut(s)	799, 938, 1334, 1966, 3306, 3405
Bsu36I	CCTNAGG	1 cut(s)	261
BtgI	CCRYGG	1 cut(s)	2394
BtrI	CACGTC	1 cut(s)	3599
BtsCI	GGATG	9 cut(s)	58, 222, 308, 587, 1392, 1605, 1689, 1779, 2374
BtsI	GCAGTG	5 cut(s)	120, 1578, 2055, 2259, 2978
BveI	ACCTGC	2 cut(s)	269, 1027
Cac8I	GCNNGC	8 cut(s)	207, 377, 622, 1136, 1435, 2947, 3008, 3544
CaiI	CAGNNNCTG	3 cut(s)	673, 2683, 3441
CciI	TCATGA	2 cut(s)	525, 2290
CfoI	GCGC	2 cut(s)	61, 2513
Cfr10I	RCCGGY	2 cut(s)	880, 2039
Cfr13I	GGNCC	7 cut(s)	148, 805, 1158, 1778, 2117, 2747, 3570
CseI	GACGC	3 cut(s)	171, 3464, 3587
Csp6I	GTAC	3 cut(s)	257, 2268, 2937
CspAI	ACCGGT	2 cut(s)	880, 2039
CviQI	GTAC	3 cut(s)	257, 2268, 2937
DdeI	CTNAG	8 cut(s)	261, 414, 465, 1743, 1862, 1911, 2331, 2586
DraIII	CACNNNGTG	2 cut(s)	1610, 3452
EaeI	YGGCCR	4 cut(s)	584, 3280, 3379, 3443
Eam1104I	CTCTTC	4 cut(s)	162, 2494, 2606, 2897
EarI	CTCTTC	4 cut(s)	162, 2494, 2606, 2897
Eco130I	CCWWGG	2 cut(s)	226, 3401
Eco147I	AGGCCT	1 cut(s)	1080
Eco24I	GRGCYC	4 cut(s)	473, 1662, 2572, 2592
Eco47I	GGWCC	3 cut(s)	805, 1158, 1778
Eco47III	AGCGCT	1 cut(s)	2512
Eco57I	CTGAAG	7 cut(s)	200, 1029, 1251, 1481, 1728, 2156, 2475
Eco81I	CCTNAGG	1 cut(s)	261
Eco88I	CYCGRG	1 cut(s)	131
Eco91I	GGTNACC	1 cut(s)	2035
EcoO65I	GGTNACC	1 cut(s)	2035
EcoT14I	CCWWGG	2 cut(s)	226, 3401
EcoT22I	ATGCAT	2 cut(s)	351, 1174
EcoT38I	GRGCYC	4 cut(s)	473, 1662, 2572, 2592
ErhI	CCWWGG	2 cut(s)	226, 3401
FalI	AAGNNNNNCTT	6 cut(s)	198, 230, 1249, 1281, 1479, 1511
FaqI	GGGAC	2 cut(s)	655, 791
FbaI	TGATCA	2 cut(s)	423, 2550
Fnu4HI	GCNGC	8 cut(s)	210, 276, 1132, 1317, 2969, 3021, 3108, 3521
FokI	GGATG	9 cut(s)	65, 209, 295, 594, 1399, 1612, 1696, 1786, 2381
FriOI	GRGCYC	4 cut(s)	473, 1662, 2572, 2592
Fsp4HI	GCNGC	8 cut(s)	210, 276, 1132, 1317, 2969, 3021, 3108, 3521
GlaI	GCGC	2 cut(s)	60, 2512
GluI	GCNGC	8 cut(s)	210, 276, 1132, 1317, 2969, 3021, 3108, 3521
GsaI	CCCAGC	2 cut(s)	88, 812
GsuI	CTGGAG	3 cut(s)	47, 1539, 2265
HaeII	RGCGCY	2 cut(s)	62, 2514
HgaI	GACGC	3 cut(s)	171, 3464, 3587
HhaI	GCGC	2 cut(s)	61, 2513
Hin6I	GCGC	2 cut(s)	59, 2511
HinP1I	GCGC	2 cut(s)	59, 2511
HincII	GTYRAC	2 cut(s)	2482, 2545
HindII	GTYRAC	2 cut(s)	2482, 2545
HindIII	AAGCTT	5 cut(s)	377, 1136, 2730, 3249, 3348
HinfI	GANTC	9 cut(s)	117, 154, 735, 854, 1915, 1991, 2753, 3136, 3143
HphI	GGTGA	7 cut(s)	785, 881, 1051, 2054, 2273, 3228, 3327
Hpy166II	GTNNAC	5 cut(s)	462, 878, 2419, 2482, 2545
Hpy8I	GTNNAC	5 cut(s)	462, 878, 2419, 2482, 2545
Hpy99I	CGWCG	1 cut(s)	165
HpyCH4IV	ACGT	2 cut(s)	777, 3598
HpyF10VI	GCNNNNNNNGC	7 cut(s)	346, 673, 1439, 1856, 3288, 3387, 3563
HpyF3I	CTNAG	8 cut(s)	261, 414, 465, 1743, 1862, 1911, 2331, 2586
HpySE526I	ACGT	2 cut(s)	777, 3598
HspAI	GCGC	2 cut(s)	59, 2511
Kpn2I	TCCGGA	1 cut(s)	1160
Ksp22I	TGATCA	2 cut(s)	423, 2550
LmnI	GCTCC	4 cut(s)	66, 2284, 2518, 2567
Lsp1109I	GCAGC	6 cut(s)	196, 262, 1303, 2980, 3032, 3532
LweI	GCATC	6 cut(s)	317, 939, 1159, 1465, 1646, 2266
MaeII	ACGT	2 cut(s)	777, 3598
MfeI	CAATTG	6 cut(s)	390, 419, 1209, 2067, 2109, 2385
MflI	RGATCY	6 cut(s)	799, 938, 1334, 1966, 3306, 3405
MhlI	GDGCHC	5 cut(s)	473, 598, 1662, 2572, 2592
MlsI	TGGCCA	4 cut(s)	586, 3282, 3381, 3445
MluNI	TGGCCA	4 cut(s)	586, 3282, 3381, 3445
MlyI	GAGTC	3 cut(s)	148, 1924, 2000
MmeI	TCCRAC	2 cut(s)	946, 1330
Mox20I	TGGCCA	4 cut(s)	586, 3282, 3381, 3445
Mph1103I	ATGCAT	2 cut(s)	351, 1174
MroI	TCCGGA	1 cut(s)	1160
MroXI	GAANNNNTTC	2 cut(s)	507, 2733
MscI	TGGCCA	4 cut(s)	586, 3282, 3381, 3445
MslI	CAYNNNNRTG	3 cut(s)	354, 524, 2552
Msp20I	TGGCCA	4 cut(s)	586, 3282, 3381, 3445
MspA1I	CMGCKG	1 cut(s)	812
MunI	CAATTG	6 cut(s)	390, 419, 1209, 2067, 2109, 2385
MvnI	CGCG	1 cut(s)	3458
MwoI	GCNNNNNNNGC	7 cut(s)	346, 673, 1439, 1856, 3288, 3387, 3563
NciI	CCSGG	3 cut(s)	3240, 3339, 3417
NheI	GCTAGC	1 cut(s)	1433
NsiI	ATGCAT	2 cut(s)	351, 1174
NspI	RCATGY	4 cut(s)	1764, 1944, 2499, 3010
PaeI	GCATGC	1 cut(s)	3010
PaeR7I	CTCGAG	1 cut(s)	131
PagI	TCATGA	2 cut(s)	525, 2290
PaqCI	CACCTGC	1 cut(s)	1027
PceI	AGGCCT	1 cut(s)	1080
PciI	ACATGT	2 cut(s)	1760, 1940
PcsI	WCGNNNNNNNCGW	2 cut(s)	112, 3200
PdmI	GAANNNNTTC	2 cut(s)	507, 2733
PfeI	GAWTC	6 cut(s)	117, 735, 854, 2753, 3136, 3143
PflMI	CCANNNNNTGG	3 cut(s)	479, 815, 3441
PfoI	TCCNGGA	2 cut(s)	3309, 3408
PinAI	ACCGGT	2 cut(s)	880, 2039
PkrI	GCNGC	8 cut(s)	211, 277, 1133, 1318, 2970, 3022, 3109, 3522
PleI	GAGTC	3 cut(s)	148, 1923, 1999
PpsI	GAGTC	3 cut(s)	148, 1923, 1999
PscI	ACATGT	2 cut(s)	1760, 1940
PshBI	ATTAAT	1 cut(s)	891
PsiI	TTATAA	2 cut(s)	663, 2918
PspEI	GGTNACC	1 cut(s)	2035
PspFI	CCCAGC	2 cut(s)	84, 808
PspPI	GGNCC	7 cut(s)	148, 805, 1158, 1778, 2117, 2747, 3570
PstI	CTGCAG	3 cut(s)	280, 678, 2868
PstNI	CAGNNNCTG	3 cut(s)	673, 2683, 3441
PsuI	RGATCY	6 cut(s)	799, 938, 1334, 1966, 3306, 3405
PvuII	CAGCTG	1 cut(s)	812
RsaI	GTAC	3 cut(s)	258, 2269, 2938
RsaNI	GTAC	3 cut(s)	257, 2268, 2937
RseI	CAYNNNNRTG	3 cut(s)	354, 524, 2552
SatI	GCNGC	8 cut(s)	210, 276, 1132, 1317, 2969, 3021, 3108, 3521
Sau96I	GGNCC	7 cut(s)	148, 805, 1158, 1778, 2117, 2747, 3570
SchI	GAGTC	3 cut(s)	148, 1924, 2000
SduI	GDGCHC	5 cut(s)	473, 598, 1662, 2572, 2592
SfaNI	GCATC	6 cut(s)	317, 939, 1159, 1465, 1646, 2266
SfcI	CTRYAG	6 cut(s)	276, 674, 1197, 1351, 1697, 2864
Sfr274I	CTCGAG	1 cut(s)	131
SinI	GGWCC	3 cut(s)	805, 1158, 1778
SlaI	CTCGAG	1 cut(s)	131
SmiMI	CAYNNNNRTG	3 cut(s)	354, 524, 2552
SmlI	CTYRAG	2 cut(s)	131, 2224
SmoI	CTYRAG	2 cut(s)	131, 2224
SphI	GCATGC	1 cut(s)	3010
SseBI	AGGCCT	1 cut(s)	1080
SsiI	CCGC	4 cut(s)	1132, 1681, 3105, 3108
SspI	AATATT	2 cut(s)	793, 889
StuI	AGGCCT	1 cut(s)	1080
StyI	CCWWGG	2 cut(s)	226, 3401
TaiI	ACGT	2 cut(s)	780, 3601
TaqI	TCGA	6 cut(s)	115, 132, 160, 2185, 2925, 3203
TaqII	GACCGA	1 cut(s)	3476
TauI	GCSGC	2 cut(s)	1134, 3110
TfiI	GAWTC	6 cut(s)	117, 735, 854, 2753, 3136, 3143
TseI	GCWGC	6 cut(s)	209, 275, 1316, 2968, 3020, 3520
TspGWI	ACGGA	2 cut(s)	121, 3463
Van91I	CCANNNNNTGG	3 cut(s)	479, 815, 3441
VpaK11BI	GGWCC	3 cut(s)	805, 1158, 1778
VspI	ATTAAT	1 cut(s)	891
XapI	RAATTY	7 cut(s)	236, 441, 1187, 2085, 2123, 2435, 3029
XbaI	TCTAGA	2 cut(s)	3254, 3353
XceI	RCATGY	4 cut(s)	1764, 1944, 2499, 3010
XcmI	CCANNNNNNNNNTGG	1 cut(s)	605
XhoI	CTCGAG	1 cut(s)	131
XmaJI	CCTAGG	1 cut(s)	3401
XmnI	GAANNNNTTC	2 cut(s)	507, 2733
Zsp2I	ATGCAT	2 cut(s)	351, 1174

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.