Rmu_sc0009677.1_g000021

Mitochondrial import inner membrane translocase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009677.1
OG0015864
Physical Location & Seq
Forward (+)
61737 .. 64689
2953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009677.1_g000021.1.cds

Sequence Viewer

Length: 597 bp
atgatcgttcgtagtcttgatcctgaaaatgatcttcttcatccaaaggatgacgacgaagatatgctagaggcagaagtgccttacttaagtacaataggcgcattattgtacttagatcaatgcacaagaccagacatctcatttacagtgaacttgttagctaagagagtctcttcccaaactcttccagcgcccaaaagcttccagagacctctgtatgataccccggattctaagctcagacaccaaacattgaacaaaatggctacaccatttcttgctggtcttgcagtagcagccactgctcttgctggtagatatggtatccaggcgtggcaagcattcaaggcacgaccacctaaaatgcgtaaattttttgaaggtggttttcagccgactatgactagaagggaagcagctcagattcttggtctcagggagagtgcgcccccagacaaggtcaaggaagcacatcgaagagtgatggttgcaaatcatcctgatgcaggtggtagtcattaccttgcttctaaaatcaacgaggctaaagatgtgatgcttaggaaaacaaagggtggtggatcagcattttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

21.93

Weight (kDa)

9.46

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015864)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35795
fragaria_vesca FvH4_7g20090
malus_domestica MD01G1130500.v1.1 MD07G1178600.v1.1
prunus_persica Prupe.2G217700_v2.0.a1
pyrus_communis pycom01g13930 pycom07g17220
rosa_chinensis RchiOBHm_Chr1g0364051
rosa_multiflora Rmu_sc0009677.1_g000021
rosa_roxburghii Rroxscaffold_4G00292700
rosa_rugosa Rorug01G0313100
rosa_samantha Rh1AG320500 Rh1BG283700 Rh1CG300100
rosa_wichuraiana Rw1G028410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 500
Acc36I ACCTGC 1 cut(s) 500
AclWI GGATC 2 cut(s) 14, 592
AcsI RAATTY 1 cut(s) 374
AfaI GTAC 2 cut(s) 94, 113
AfiI CCNNNNNNNGG 2 cut(s) 460, 509
AflII CTTAAG 1 cut(s) 88
AgsI TTSAA 3 cut(s) 259, 349, 383
AjnI CCWGG 1 cut(s) 330
AluBI AGCT 4 cut(s) 164, 204, 241, 422
AluI AGCT 4 cut(s) 164, 204, 241, 422
Alw26I GTCTC 3 cut(s) 178, 205, 440
AlwI GGATC 2 cut(s) 14, 592
AlwNI CAGNNNCTG 1 cut(s) 305
ApeKI GCWGC 2 cut(s) 299, 419
ApoI RAATTY 1 cut(s) 374
AspLEI GCGC 3 cut(s) 104, 196, 451
AsuC2I CCSGG 1 cut(s) 230
BbvI GCAGC 2 cut(s) 311, 431
BccI CCATC 1 cut(s) 481
BciT130I CCWGG 1 cut(s) 332
BciVI GTATCC 1 cut(s) 338
BcnI CCSGG 1 cut(s) 230
BcoDI GTCTC 3 cut(s) 178, 205, 440
BfaI CTAG 2 cut(s) 68, 408
BfoI RGCGCY 1 cut(s) 197
BfrI CTTAAG 1 cut(s) 88
BfuAI ACCTGC 1 cut(s) 500
BfuI GTATCC 1 cut(s) 338
BisI GCNGC 2 cut(s) 300, 420
BlsI GCNGC 2 cut(s) 301, 421
Bme1390I CCNGG 2 cut(s) 230, 332
BmrFI CCNGG 2 cut(s) 230, 332
BmsI GCATC 2 cut(s) 496, 549
Bpu10I CCTNAGC 1 cut(s) 563
BpuMI CCSGG 1 cut(s) 230
BsaI GGTCTC 2 cut(s) 205, 440
BsaJI CCNNGG 1 cut(s) 228
Bsc4I CCNNNNNNNGG 2 cut(s) 460, 509
BseBI CCWGG 1 cut(s) 332
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 3 cut(s) 40, 55, 499
BseLI CCNNNNNNNGG 2 cut(s) 460, 509
BseMII CTCAG 3 cut(s) 256, 437, 451
BseXI GCAGC 2 cut(s) 311, 431
BsiSI CCGG 1 cut(s) 230
BslI CCNNNNNNNGG 2 cut(s) 460, 509
BsmAI GTCTC 3 cut(s) 178, 205, 440
BsmI GAATGC 1 cut(s) 344
Bso31I GGTCTC 2 cut(s) 205, 440
Bsp143I GATC 5 cut(s) 3, 19, 31, 118, 584
BspCNI CTCAG 3 cut(s) 255, 436, 450
BspMI ACCTGC 1 cut(s) 500
BspPI GGATC 2 cut(s) 14, 592
BspTI CTTAAG 1 cut(s) 88
BspTNI GGTCTC 2 cut(s) 205, 440
BssECI CCNNGG 1 cut(s) 228
BssMI GATC 5 cut(s) 3, 19, 31, 118, 584
Bst2UI CCWGG 1 cut(s) 332
Bst4CI ACNGT 1 cut(s) 151
Bst6I CTCTTC 3 cut(s) 181, 192, 475
BstAFI CTTAAG 1 cut(s) 88
BstAPI GCANNNNNTGC 1 cut(s) 305
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 7 cut(s) 115, 165, 237, 242, 423, 437, 563
BstENI CCTNNNNNAGG 1 cut(s) 507
BstF5I GGATG 3 cut(s) 40, 55, 499
BstH2I RGCGCY 1 cut(s) 197
BstHHI GCGC 3 cut(s) 104, 196, 451
BstKTI GATC 5 cut(s) 6, 22, 34, 121, 587
BstMAI GTCTC 3 cut(s) 178, 205, 440
BstMBI GATC 5 cut(s) 3, 19, 31, 118, 584
BstMWI GCNNNNNNNGC 5 cut(s) 290, 299, 305, 341, 350
BstNI CCWGG 1 cut(s) 332
BstSCI CCNGG 2 cut(s) 228, 330
BstV1I GCAGC 2 cut(s) 311, 431
BsuI GTATCC 1 cut(s) 338
BtsCI GGATG 3 cut(s) 40, 55, 499
BtsI GCAGTG 1 cut(s) 303
BtsIMutI CAGTG 2 cut(s) 156, 303
BveI ACCTGC 1 cut(s) 500
Cac8I GCNNGC 1 cut(s) 342
CaiI CAGNNNCTG 1 cut(s) 305
CfoI GCGC 3 cut(s) 104, 196, 451
Csp6I GTAC 2 cut(s) 93, 112
CspCI CAANNNNNGTGG 2 cut(s) 292, 327
CviJI RGCY 8 cut(s) 164, 204, 241, 269, 302, 397, 422, 548
CviKI_1 RGCY 8 cut(s) 164, 204, 241, 269, 302, 397, 422, 548
CviQI GTAC 2 cut(s) 93, 112
DdeI CTNAG 7 cut(s) 115, 165, 237, 242, 423, 437, 563
DpnI GATC 5 cut(s) 5, 21, 33, 120, 586
DpnII GATC 5 cut(s) 3, 19, 31, 118, 584
Eam1104I CTCTTC 3 cut(s) 181, 192, 475
EarI CTCTTC 3 cut(s) 181, 192, 475
Eco31I GGTCTC 2 cut(s) 205, 440
EcoNI CCTNNNNNAGG 1 cut(s) 507
EcoRII CCWGG 1 cut(s) 330
FaiI YATR 4 cut(s) 65, 222, 324, 404
Fnu4HI GCNGC 2 cut(s) 300, 420
FokI GGATG 3 cut(s) 27, 62, 486
Fsp4HI GCNGC 2 cut(s) 300, 420
FspBI CTAG 2 cut(s) 68, 408
GlaI GCGC 3 cut(s) 103, 195, 450
GluI GCNGC 2 cut(s) 300, 420
HaeII RGCGCY 1 cut(s) 197
HapII CCGG 1 cut(s) 230
HhaI GCGC 3 cut(s) 104, 196, 451
Hin6I GCGC 3 cut(s) 102, 194, 449
HinP1I GCGC 3 cut(s) 102, 194, 449
HindIII AAGCTT 1 cut(s) 202
HinfI GANTC 3 cut(s) 171, 233, 427
HpaII CCGG 1 cut(s) 230
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 2 cut(s) 245, 426
Hpy188III TCNNGA 4 cut(s) 17, 23, 208, 503
Hpy8I GTNNAC 1 cut(s) 154
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 2 cut(s) 377, 405
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4V TGCA 4 cut(s) 126, 293, 494, 509
HpyF10VI GCNNNNNNNGC 5 cut(s) 290, 299, 305, 341, 350
HpyF3I CTNAG 7 cut(s) 115, 165, 237, 242, 423, 437, 563
HspAI GCGC 3 cut(s) 102, 194, 449
Kzo9I GATC 5 cut(s) 3, 19, 31, 118, 584
Lsp1109I GCAGC 2 cut(s) 311, 431
LweI GCATC 2 cut(s) 496, 549
MaeI CTAG 2 cut(s) 68, 408
MalI GATC 5 cut(s) 5, 21, 33, 120, 586
MboI GATC 5 cut(s) 3, 19, 31, 118, 584
MboII GAAGA 6 cut(s) 26, 29, 71, 168, 179, 492
MluCI AATT 1 cut(s) 374
MlyI GAGTC 1 cut(s) 180
MnlI CCTC 3 cut(s) 64, 225, 538
MseI TTAA 1 cut(s) 89
MslI CAYNNNNRTG 1 cut(s) 504
MspCI CTTAAG 1 cut(s) 88
MspI CCGG 1 cut(s) 230
MspR9I CCNGG 2 cut(s) 230, 332
Mva1269I GAATGC 1 cut(s) 344
MvaI CCWGG 1 cut(s) 332
MwoI GCNNNNNNNGC 5 cut(s) 290, 299, 305, 341, 350
NciI CCSGG 1 cut(s) 230
NdeII GATC 5 cut(s) 3, 19, 31, 118, 584
PaqCI CACCTGC 1 cut(s) 500
PctI GAATGC 1 cut(s) 344
PfeI GAWTC 2 cut(s) 233, 427
PflFI GACNNNGTC 1 cut(s) 461
PkrI GCNGC 2 cut(s) 301, 421
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
Psp6I CCWGG 1 cut(s) 330
PspGI CCWGG 1 cut(s) 330
PstNI CAGNNNCTG 1 cut(s) 305
PsyI GACNNNGTC 1 cut(s) 461
RsaI GTAC 2 cut(s) 94, 113
RsaNI GTAC 2 cut(s) 93, 112
RseI CAYNNNNRTG 1 cut(s) 504
SaqAI TTAA 1 cut(s) 89
SatI GCNGC 2 cut(s) 300, 420
Sau3AI GATC 5 cut(s) 3, 19, 31, 118, 584
SchI GAGTC 1 cut(s) 180
ScrFI CCNGG 2 cut(s) 230, 332
SfaNI GCATC 2 cut(s) 496, 549
SmiMI CAYNNNNRTG 1 cut(s) 504
SmlI CTYRAG 1 cut(s) 88
SmoI CTYRAG 1 cut(s) 88
Sse9I AATT 1 cut(s) 374
SspMI CTAG 2 cut(s) 68, 408
StyD4I CCNGG 2 cut(s) 228, 330
TaaI ACNGT 1 cut(s) 151
TaqI TCGA 1 cut(s) 478
TasI AATT 1 cut(s) 374
TatI WGTACW 2 cut(s) 92, 111
TfiI GAWTC 2 cut(s) 233, 427
Tru1I TTAA 1 cut(s) 89
Tru9I TTAA 1 cut(s) 89
TscAI CASTG 2 cut(s) 156, 310
TseI GCWGC 2 cut(s) 299, 419
TspDTI ATGAA 1 cut(s) 29
TspRI CASTG 2 cut(s) 156, 310
Tth111I GACNNNGTC 1 cut(s) 461
Vha464I CTTAAG 1 cut(s) 88
XagI CCTNNNNNAGG 1 cut(s) 507
XapI RAATTY 1 cut(s) 374
XspI CTAG 2 cut(s) 68, 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.