Rmu_sc0035374.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0035374.1
OG0017466
Physical Location & Seq
Reverse (-)
15 .. 777
763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0035374.1_g000001.1.cds

Sequence Viewer

Length: 669 bp
atgaattaccttttcagagaatcatctaggatcagctggggcaatgccaatacacaaaattcttatagtaacaggggacaactaaagcttacagcgctcaggggacaattgaaggaacagggagaagagcttacaacgctcaagggaaaactgaatgaatgtgaagaatccataaagaaattggacaagaccattgaagatcagaaagagctgatttctgggccgagttcacctgcaattgctaaaactgacctggacagagagattgacgaaggggtggactggtggatctttgttcagagagtgatgtgtgagttggcactcacagatgttgaaactcagttgaaggaagggaaagataaacacatttgtacaattgtttttggcgctctggtcgttctgtactctgcgcttgaatgtgtccgttctcacaagaaaaaaccatggattcagaatggagaactgaaagtgaaaaatattgtttgggaaacagaactttacggtctgttgttctcttttgtgcagctaattatgtatattgtggcaatttggaagccagtggccattagagtatactttcagaccatccttttcaacctctatgtcttcctcagcaagatgtacaagcgctcccaccgtatcaaaaaggtcactgtagatgttgtttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

25.74

Weight (kDa)

8.9

Isoelectric Point (pI)

32.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 241
Acc36I ACCTGC 1 cut(s) 241
AccI GTMKAC 1 cut(s) 573
AclWI GGATC 2 cut(s) 38, 296
AcoI YGGCCR 1 cut(s) 561
AcsI RAATTY 1 cut(s) 58
AfaI GTAC 3 cut(s) 373, 404, 623
AfeI AGCGCT 2 cut(s) 96, 629
AgsI TTSAA 6 cut(s) 112, 197, 335, 346, 416, 595
AjnI CCWGG 1 cut(s) 252
AluBI AGCT 5 cut(s) 36, 88, 130, 211, 526
AluI AGCT 5 cut(s) 36, 88, 130, 211, 526
AlwI GGATC 2 cut(s) 38, 296
Aor51HI AGCGCT 2 cut(s) 96, 629
AoxI GGCC 2 cut(s) 221, 561
ApeKI GCWGC 1 cut(s) 523
ApoI RAATTY 1 cut(s) 58
AspLEI GCGC 4 cut(s) 97, 389, 412, 630
AspS9I GGNCC 1 cut(s) 221
AsuHPI GGTGA 1 cut(s) 222
BalI TGGCCA 1 cut(s) 563
BbsI GAAGAC 1 cut(s) 598
BbvCI CCTCAGC 1 cut(s) 611
BbvI GCAGC 1 cut(s) 535
BccI CCATC 1 cut(s) 593
BciT130I CCWGG 1 cut(s) 254
BfaI CTAG 1 cut(s) 27
BfmI CTRYAG 1 cut(s) 654
BfoI RGCGCY 3 cut(s) 98, 390, 631
BfuAI ACCTGC 1 cut(s) 241
BisI GCNGC 1 cut(s) 524
BlsI GCNGC 1 cut(s) 525
Bme1390I CCNGG 1 cut(s) 254
BmgT120I GGNCC 1 cut(s) 221
BmrFI CCNGG 1 cut(s) 254
BpiI GAAGAC 1 cut(s) 598
Bpu10I CCTNAGC 2 cut(s) 98, 611
BpuEI CTTGAG 1 cut(s) 125
BsaJI CCNNGG 1 cut(s) 443
BsaXI ACNNNNNCTCC 2 cut(s) 614, 644
Bse1I ACTGG 2 cut(s) 287, 557
Bse3DI GCAATG 1 cut(s) 49
BseBI CCWGG 1 cut(s) 254
BseDI CCNNGG 1 cut(s) 443
BseGI GGATG 1 cut(s) 585
BseMI GCAATG 1 cut(s) 49
BseMII CTCAG 3 cut(s) 112, 353, 625
BseNI ACTGG 2 cut(s) 287, 557
BseXI GCAGC 1 cut(s) 535
BseYI CCCAGC 1 cut(s) 36
BsgI GTGCAG 1 cut(s) 542
BshFI GGCC 2 cut(s) 223, 563
BslFI GGGAC 2 cut(s) 90, 117
BsmFI GGGAC 2 cut(s) 90, 117
BsnI GGCC 2 cut(s) 223, 563
Bsp1407I TGTACA 2 cut(s) 371, 621
Bsp143I GATC 3 cut(s) 30, 199, 288
Bsp19I CCATGG 1 cut(s) 443
BspANI GGCC 2 cut(s) 223, 563
BspCNI CTCAG 3 cut(s) 111, 352, 624
BspMI ACCTGC 1 cut(s) 241
BspPI GGATC 2 cut(s) 38, 296
BspQI GCTCTTC 1 cut(s) 120
BsrDI GCAATG 1 cut(s) 49
BsrGI TGTACA 2 cut(s) 371, 621
BsrI ACTGG 2 cut(s) 287, 557
BssECI CCNNGG 1 cut(s) 443
BssMI GATC 3 cut(s) 30, 199, 288
BssNAI GTATAC 1 cut(s) 574
BssT1I CCWWGG 1 cut(s) 443
Bst1107I GTATAC 1 cut(s) 574
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 3 cut(s) 503, 638, 655
Bst6I CTCTTC 1 cut(s) 120
BstAUI TGTACA 2 cut(s) 371, 621
BstDEI CTNAG 3 cut(s) 98, 339, 611
BstDSI CCRYGG 1 cut(s) 443
BstF5I GGATG 1 cut(s) 585
BstH2I RGCGCY 3 cut(s) 98, 390, 631
BstHHI GCGC 4 cut(s) 97, 389, 412, 630
BstKTI GATC 3 cut(s) 33, 202, 291
BstMBI GATC 3 cut(s) 30, 199, 288
BstMWI GCNNNNNNNGC 2 cut(s) 94, 136
BstNI CCWGG 1 cut(s) 254
BstSCI CCNGG 1 cut(s) 252
BstSFI CTRYAG 1 cut(s) 654
BstV1I GCAGC 1 cut(s) 535
BstV2I GAAGAC 1 cut(s) 598
BstX2I RGATCY 1 cut(s) 288
BstYI RGATCY 1 cut(s) 288
BstZ17I GTATAC 1 cut(s) 574
BsuRI GGCC 2 cut(s) 223, 563
BtgI CCRYGG 1 cut(s) 443
BtsCI GGATG 1 cut(s) 585
BtsIMutI CAGTG 2 cut(s) 564, 651
BveI ACCTGC 1 cut(s) 241
CfoI GCGC 4 cut(s) 97, 389, 412, 630
Cfr13I GGNCC 1 cut(s) 221
Csp6I GTAC 3 cut(s) 372, 403, 622
CviAII CATG 1 cut(s) 444
CviJI RGCY 8 cut(s) 36, 88, 130, 211, 223, 526, 556, 563
CviKI_1 RGCY 8 cut(s) 36, 88, 130, 211, 223, 526, 556, 563
CviQI GTAC 3 cut(s) 372, 403, 622
DdeI CTNAG 3 cut(s) 98, 339, 611
DpnI GATC 3 cut(s) 32, 201, 290
DpnII GATC 3 cut(s) 30, 199, 288
EaeI YGGCCR 1 cut(s) 561
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 443
Eco47III AGCGCT 2 cut(s) 96, 629
EcoRII CCWGG 1 cut(s) 252
EcoT14I CCWWGG 1 cut(s) 443
ErhI CCWWGG 1 cut(s) 443
FaeI CATG 1 cut(s) 447
FaiI YATR 7 cut(s) 66, 173, 445, 533, 537, 574, 603
FaqI GGGAC 2 cut(s) 90, 117
FatI CATG 1 cut(s) 443
FblI GTMKAC 1 cut(s) 573
Fnu4HI GCNGC 1 cut(s) 524
FokI GGATG 1 cut(s) 572
Fsp4HI GCNGC 1 cut(s) 524
FspBI CTAG 1 cut(s) 27
GlaI GCGC 4 cut(s) 96, 388, 411, 629
GluI GCNGC 1 cut(s) 524
GsaI CCCAGC 1 cut(s) 40
HaeII RGCGCY 3 cut(s) 98, 390, 631
HaeIII GGCC 2 cut(s) 223, 563
HhaI GCGC 4 cut(s) 97, 389, 412, 630
Hin1II CATG 1 cut(s) 447
Hin6I GCGC 4 cut(s) 95, 387, 410, 628
HinP1I GCGC 4 cut(s) 95, 387, 410, 628
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 3 cut(s) 20, 167, 448
HphI GGTGA 1 cut(s) 222
Hpy166II GTNNAC 3 cut(s) 230, 280, 574
Hpy188I TCNGA 5 cut(s) 17, 204, 300, 453, 582
Hpy8I GTNNAC 3 cut(s) 230, 280, 574
HpyAV CCTTC 4 cut(s) 106, 266, 340, 344
HpyCH4III ACNGT 3 cut(s) 503, 638, 655
HpyCH4V TGCA 2 cut(s) 236, 523
HpyF10VI GCNNNNNNNGC 2 cut(s) 94, 136
HpyF3I CTNAG 3 cut(s) 98, 339, 611
Hsp92II CATG 1 cut(s) 447
HspAI GCGC 4 cut(s) 95, 387, 410, 628
Kzo9I GATC 3 cut(s) 30, 199, 288
LguI GCTCTTC 1 cut(s) 120
LmnI GCTCC 1 cut(s) 635
Lsp1109I GCAGC 1 cut(s) 535
MaeI CTAG 1 cut(s) 27
MaeIII GTNAC 2 cut(s) 68, 649
MalI GATC 3 cut(s) 32, 201, 290
MboI GATC 3 cut(s) 30, 199, 288
MboII GAAGA 4 cut(s) 137, 176, 209, 598
MfeI CAATTG 3 cut(s) 107, 237, 375
MflI RGATCY 1 cut(s) 288
MlsI TGGCCA 1 cut(s) 563
MluCI AATT 8 cut(s) 4, 58, 107, 179, 237, 375, 528, 546
MluNI TGGCCA 1 cut(s) 563
MnlI CCTC 2 cut(s) 608, 620
Mox20I TGGCCA 1 cut(s) 563
MscI TGGCCA 1 cut(s) 563
Msp20I TGGCCA 1 cut(s) 563
MspA1I CMGCKG 1 cut(s) 36
MspR9I CCNGG 1 cut(s) 254
MunI CAATTG 3 cut(s) 107, 237, 375
MvaI CCWGG 1 cut(s) 254
MwoI GCNNNNNNNGC 2 cut(s) 94, 136
NcoI CCATGG 1 cut(s) 443
NdeII GATC 3 cut(s) 30, 199, 288
NlaIII CATG 1 cut(s) 447
NmeAIII GCCGAG 1 cut(s) 249
NmuCI GTSAC 1 cut(s) 649
PaqCI CACCTGC 1 cut(s) 241
PciSI GCTCTTC 1 cut(s) 120
PfeI GAWTC 3 cut(s) 20, 167, 448
PkrI GCNGC 1 cut(s) 525
Psp6I CCWGG 1 cut(s) 252
PspFI CCCAGC 1 cut(s) 36
PspGI CCWGG 1 cut(s) 252
PspPI GGNCC 1 cut(s) 221
PsuI RGATCY 1 cut(s) 288
PvuII CAGCTG 1 cut(s) 36
RsaI GTAC 3 cut(s) 373, 404, 623
RsaNI GTAC 3 cut(s) 372, 403, 622
SapI GCTCTTC 1 cut(s) 120
SatI GCNGC 1 cut(s) 524
Sau3AI GATC 3 cut(s) 30, 199, 288
Sau96I GGNCC 1 cut(s) 221
ScrFI CCNGG 1 cut(s) 254
SfcI CTRYAG 1 cut(s) 654
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
Sse9I AATT 8 cut(s) 4, 58, 107, 179, 237, 375, 528, 546
SspI AATATT 1 cut(s) 478
SspMI CTAG 1 cut(s) 27
StyD4I CCNGG 1 cut(s) 252
StyI CCWWGG 1 cut(s) 443
TaaI ACNGT 3 cut(s) 503, 638, 655
TasI AATT 8 cut(s) 4, 58, 107, 179, 237, 375, 528, 546
TatI WGTACW 3 cut(s) 371, 402, 621
TfiI GAWTC 3 cut(s) 20, 167, 448
TscAI CASTG 2 cut(s) 564, 658
TseFI GTSAC 1 cut(s) 649
TseI GCWGC 1 cut(s) 523
Tsp45I GTSAC 1 cut(s) 649
TspDTI ATGAA 2 cut(s) 17, 171
TspGWI ACGGA 1 cut(s) 413
TspRI CASTG 2 cut(s) 564, 658
XapI RAATTY 1 cut(s) 58
XcmI CCANNNNNNNNNTGG 1 cut(s) 178
XmiI GTMKAC 1 cut(s) 573
XspI CTAG 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.